LOCUS       NZ_CP070260            15470 bp    DNA     linear   CON 30-MAR-2024
DEFINITION  Streptomyces californicus strain FDAARGOS_1210 chromosome.
ACCESSION   NZ_CP070260
VERSION     NZ_CP070260.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces californicus
  ORGANISM  Streptomyces californicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7919564)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7919564)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 624.55x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016906265.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 09:02:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,147
            Genes (coding)                    :: 7,032
            CDSs (with protein)               :: 7,032
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 36 of 115
            Pseudo Genes (incomplete)         :: 97 of 115
            Pseudo Genes (internal stop)      :: 12 of 115
            Pseudo Genes (multiple problems)  :: 27 of 115
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:25:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4305353
            Orig. end                         :: 4320823
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070260.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..15470
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /candidate_cluster_numbers="3"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /product="ectoine"
                     /region_number="19"
                     /rules="AfsA"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..15470
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /detection_rules="ectoine_synt"
                     /kind="neighbouring"
                     /product="butyrolactone"
                     /product="ectoine"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     cand_cluster    1..10879
                     /candidate_cluster_number="2"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10879
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5879](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5879
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(482..496)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            763..1242
                     /locus_tag="I6J40_RS18710"
                     /old_locus_tag="I6J40_18700"
     CDS             763..1242
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1240: riboflavin biosynthesis protein RibD"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763177.1"
                     /locus_tag="I6J40_RS18710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18700"
                     /product="nucleoside deaminase"
                     /protein_id="WP_030189414.1"
                     /transl_table=11
                     /translation="MGTHDSELMGYAHEAIRLSREHVAQGGIPFSGVVVGGGRILGTGF
                     NRVREDNDPTAHAEVVAIREATTRHGIRAVAGATLIASGEPCALCYMAARFAGIGHIVH
                     AADRRTAARYGFDYTSTYTLFAEDPERWPLKVTALHLPEAEQPFQDFLTMDRSRY"
     gene            1420..1740
                     /locus_tag="I6J40_RS18715"
                     /old_locus_tag="I6J40_18705"
     CDS             1420..1740
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967532.1"
                     /locus_tag="I6J40_RS18715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18705"
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_030113432.1"
                     /transl_table=11
                     /translation="MHWLYLAIAVAFEITVAIAAGKAEGFKNRKWTGITLASGAAATFF
                     LSKALLTFDVGVGYALWTSVAGVGITVLGALFFGQRLNINKAIGILLVIGGVVGLQLSG
                     SA"
     gene            1823..2239
                     /locus_tag="I6J40_RS18720"
                     /old_locus_tag="I6J40_18710"
     CDS             1823..2239
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452955.1"
                     /locus_tag="I6J40_RS18720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18710"
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_030113431.1"
                     /transl_table=11
                     /translation="MSNAAKSSTNAWLSLLLAGVFEIGYALAVGGSEGFTQLTWSLVAV
                     VFFLLTLYALSLALRTIDVSIGYAVWAGIGAVGAAVFGPLFFDETLTLAKGIWLAVIIV
                     GVIWLKLADRTKPEAVAAPAAEPQATNRPGALQV"
     gene            complement(2334..3104)
                     /locus_tag="I6J40_RS18725"
                     /old_locus_tag="I6J40_18715"
     CDS             complement(2334..3104)
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (11-175). E-value: 8.3e-14.
                     Score: 43.8. Matches aSDomain:
                     nrpspksdomains_I6J40_RS18725_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452957.1"
                     /locus_tag="I6J40_RS18725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18715"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_030189416.1"
                     /sec_met_domain="adh_short (E-value: 1.3e-37, bitscore:
                     128.2, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 7.1e-31, bitscore:
                     106.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRPGGPLLAGKVALITGASSGIGAAAARLFADEGAAVVLVARRAE
                     RIEEAAERIRKDGGRAAAVPGDVTRRADMDRAVAVAAERFGRLDCAFNNAGHASAGTLL
                     HELSDDEFDRTLDVNLRGVWNCLRAQLPALLAAGGGAIVNTSSVAGVRATSASAPYVAA
                     KHAVLGLTRAAAAEYGEYGIRVNALVVGSTDTEMMDGVLAGGPAVRAGFGGKAIQRRLA
                     DPVEIARTAAWLCSDASSFTTGAALAVDGGRTAV"
     aSDomain        complement(2580..3071)
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_I6J40_RS18725_PKS_KR.1"
                     /evalue="8.30E-14"
                     /label="I6J40_RS18725_PKS_KR.1"
                     /locus_tag="I6J40_RS18725"
                     /protein_end="175"
                     /protein_start="11"
                     /score="43.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VALITGASSGIGAAAARLFADEGAAVVLVARRAERIEEAAERIRK
                     DGGRAAAVPGDVTRRADMDRAVAVAAERFGRLDCAFNNAGHASAGTLLHELSDDEFDRT
                     LDVNLRGVWNCLRAQLPALLAAGGGAIVNTSSVAGVRATSASAPYVAAKHAVLGLTRAA
                     A"
     CDS_motif       complement(2976..3065)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_I6J40_RS18725_0001"
                     /evalue="1.50E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="I6J40_RS18725"
                     /protein_end="43"
                     /protein_start="13"
                     /score="17.6"
                     /tool="antismash"
                     /translation="LITGASSGIGAAAARLFADEGAAVVLVARR"
     gene            complement(3101..3814)
                     /locus_tag="I6J40_RS18730"
                     /old_locus_tag="I6J40_18720"
     CDS             complement(3101..3814)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763173.1"
                     /locus_tag="I6J40_RS18730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18720"
                     /product="hypothetical protein"
                     /protein_id="WP_030189418.1"
                     /transl_table=11
                     /translation="MLTLRLAALNIDGVLLNDTFSPVIHHFITTRGGTYSAELERSVFS
                     QPQHIAGRLLAAATGGSMSGEEALAAYFEDRAAHLDRHPVTITPGTEELLVRLRAAGLR
                     TVCYGGLGKDHFDRHLGRYAELFDGPGYVCTNDFRPGLKEITRDVFGLRPEQALFVDDV
                     AKVADVARELGAGFVGRPSTFVHSHQRALMEESGVCHLIGGLDEIDDGLVRRIDRDLAS
                     GTFWDAAPVAAGTAA"
     gene            3875..4045
                     /locus_tag="I6J40_RS18735"
                     /old_locus_tag="I6J40_18725"
     CDS             3875..4045
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I6J40_RS18735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="I6J40_18725"
                     /product="hypothetical protein"
                     /protein_id="WP_157851940.1"
                     /transl_table=11
                     /translation="MPNVRGMSGVLVVLVVPKGRPVGSAGRVRLWAERPGRPLRSGRRR
                     TAVSRGVSALP"
     gene            complement(4073..4705)
                     /locus_tag="I6J40_RS18740"
                     /old_locus_tag="I6J40_18730"
     CDS             complement(4073..4705)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452959.1"
                     /locus_tag="I6J40_RS18740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18730"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_030113428.1"
                     /transl_table=11
                     /translation="MAQPKQERAEQTRRALLTAAAEVFDEFGYAGASITRILKRAGVTA
                     GALYFHFGSKEDLAKAVMNSQPETLVPLLAAGGLQRLIDLTLVWSWQLQRDPLLRAGVR
                     LTNEQASINGTLNADPYENFRGIMVGCLHEALESGELQPGVDPVVVAEFVVAACTGMQM
                     YSNVVSGRSDLPERTQQMWNLILPGLAVPSVITRAETSPARGSALMP"
     misc_feature    4843..4856
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.03"
                     /tool="antismash"
     misc_feature    4857..4872
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.81"
                     /tool="antismash"
     misc_feature    4866..4875
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.85"
                     /tool="antismash"
     gene            5001..5879
                     /locus_tag="I6J40_RS18745"
                     /old_locus_tag="I6J40_18735"
     CDS             5001..5879
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452960.1"
                     /locus_tag="I6J40_RS18745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18735"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_050494444.1"
                     /sec_met_domain="AfsA (E-value: 2.1e-21, bitscore: 74.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHQWQVHKLRGEEVLLTSWLEASPNQYVVTARWPKRHDFYRPAGG
                     SYDPLLLAETVRQTVPLLSHAVHDVPREYKQAWEDFSFAVEPLASLVTTGEDEVRMVVS
                     CSDVIRRGARFAGMTMDIDVYLGKRLMGIAHTRFNNQPAAIYRRLRGSYADLDAAQEGC
                     LPVGPPVEPHRVGRDRVEDVVLSPALGGERPGRWLLRVDPSHPVLFDHVVDHVPGMLLL
                     EAARQAAHDVAGPGAAVIGMKTDFIRYVEFDAPTVVVAGEPLSAGTGRRRIPLRVEQHG
                     VQVFTAEVTVH"
     misc_feature    5058..5060
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     cand_cluster    5096..15470
                     /candidate_cluster_number="3"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    5096..15470
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10095:10470](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(10096..10470)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="2"
     gene            complement(6007..6759)
                     /locus_tag="I6J40_RS18750"
                     /old_locus_tag="I6J40_18740"
     CDS             complement(6007..6759)
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: ACPS (134-193). E-value: 3e-07. Score:
                     22.2. Matches aSDomain:
                     nrpspksdomains_I6J40_RS18750_ACPS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1012: 4'-phosphopantetheinyl transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763171.1"
                     /locus_tag="I6J40_RS18750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18740"
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="WP_030189424.1"
                     /transl_table=11
                     /translation="MTHDFTWSDAPPGEVLDPVGIWIVRAPAGQRLPPDGSAPPWLDEK
                     ERSRLASIPQQSGRESYAFAHWALRSVLGSSLGCSPGSVGFLRQPCPGCGGAHGRPAIR
                     SPAPDPAGEPLEFSMSHSGDHVAIAVAEATIGIDIESWPTPESVAGYLPFLHPREQQWL
                     GGRAGDELVRDFARLWTRKEAMAKATGQGMAAVMNRLDLTGQPLGWRVRQLLAPPGYEA
                     SFAVPASVHVSVTVYEELPQPMPELART"
     aSDomain        complement(6181..6357)
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_I6J40_RS18750_ACPS.1"
                     /evalue="3.00E-07"
                     /label="I6J40_RS18750_ACPS.1"
                     /locus_tag="I6J40_RS18750"
                     /protein_end="193"
                     /protein_start="134"
                     /score="22.2"
                     /tool="antismash"
                     /translation="IGIDIESWPTPESVAGYLPFLHPREQQWLGGRAGDELVRDFARLW
                     TRKEAMAKATGQGM"
     gene            complement(6815..7057)
                     /locus_tag="I6J40_RS18755"
                     /old_locus_tag="I6J40_18745"
     CDS             complement(6815..7057)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489549.1"
                     /locus_tag="I6J40_RS18755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18745"
                     /product="DUF6253 family protein"
                     /protein_id="WP_030113424.1"
                     /transl_table=11
                     /translation="MSLLPAYGYQAAVQETCDTPEYHIPLVCWHDDDTGVRGMVLYRGA
                     LRPAEQVPGFRRYLASEDLGQYATTGPWPLLAATA"
     gene            complement(7054..7869)
                     /locus_tag="I6J40_RS18760"
                     /old_locus_tag="I6J40_18750"
     CDS             complement(7054..7869)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763169.1"
                     /locus_tag="I6J40_RS18760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18750"
                     /product="maleate cis-trans isomerase"
                     /protein_id="WP_030113423.1"
                     /transl_table=11
                     /translation="MTQFQAREDGWGAVARVGVVVPHADVGPESELQAMAPRDVFIHGS
                     RVHFGAMRPGGEMDPKIPHDPVRAFIDPPFIDDAVELLAASPLDSIALGFTSSSYVLGA
                     DGEQKLFERLAERTRGIPLTGTAHAAAAGFRALGAERIALVNPPWFDDELSALGASYFG
                     ERGFDVVHHAPCGLPSNQKLITPEALVKWIESDVAPHRPDAVLVAGNGIRAVGTIAGLE
                     ETVGFPVLTANQVLFWHALHAAGGSGAAARITDYGALFQVVPEAGSVAR"
     gene            complement(8110..8859)
                     /locus_tag="I6J40_RS18765"
                     /old_locus_tag="I6J40_18755"
     CDS             complement(8110..8859)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489547.1"
                     /locus_tag="I6J40_RS18765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18755"
                     /product="transglutaminase family protein"
                     /protein_id="WP_030189425.1"
                     /transl_table=11
                     /translation="MTETLTEATSAQPAADAAAERSTAEDLARLTEATDFLDHRHPTVQ
                     AFVDKALKGTDRETAGPVELAVTLYYAVRDGIHYEVYGADLSPEGLRASSIITGGKGFC
                     LHKSVLYAACCRAVGIPARLHYSDVRNHLASDRLRSYIGGDVFFHGLATVYLDGRWLQV
                     TPVFNKLLCRLYGMTPLEFDGRSDSLYHPFDAQGRQSMEFLTDHGDFDDVPYAFVMENM
                     RRKHPKFLDEGGTGTVSGGSLADEATA"
     gene            complement(8874..10004)
                     /locus_tag="I6J40_RS18770"
                     /old_locus_tag="I6J40_18760"
     CDS             complement(8874..10004)
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489546.1"
                     /locus_tag="I6J40_RS18770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18760"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_030113421.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 2.1e-17, bitscore:
                     62.4, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 4.3e-26, bitscore:
                     89.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 5.8e-32, bitscore:
                     109.6, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAENDTDTYGLTAPYADELNDDVTRWDRAGEFPHEKWKPLQRSG
                     LFTLPFAPEHGGAGRTLTETMAALEGLGHTSRDAGLNFSASTQIVSVGIPLQAFGSAEL
                     RERYLPRVTSGEAITAHAITEPGHGSDVMNIRTTAVRDGDDYVLNGGKLFITNAPVADL
                     FLLYVRTGKPGAFGLSTFLAERSTPGLTVGEPLDTMGLRTSPISEVTLTDVRLPVANRL
                     GSEGAGFLIMDYVMKREVLFSFAVNLGEMTHRLKRVVEHATTRQQFGASIGKNQAVAHK
                     IADMRIAVETARKWLYDTGRKVEQGKDASLDVAATKIVVSEANQLTARHAIQIFGARGY
                     LTDTGIERELRNATAGSIYSGTNEIQRNCIAALMGL"
     gene            complement(10096..10470)
                     /locus_tag="I6J40_RS18775"
                     /old_locus_tag="I6J40_18765"
     CDS             complement(10096..10470)
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452967.1"
                     /locus_tag="I6J40_RS18775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18765"
                     /product="ectoine synthase"
                     /protein_id="WP_030189426.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.3e-24, bitscore:
                     85.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLSDVPAVEWGSGTSHRLLTEQDGMGFTVCETLVRQGTSSA
                     LQYLGHLEACYCIGGSGEVVTTDGTRHKIKTGTIYALDKNDAHHLIASDDEDLLLVSVF
                     SPALRGDEKHSLSDDGFSRY"
     gene            complement(10590..10850)
                     /locus_tag="I6J40_RS18780"
                     /old_locus_tag="I6J40_18770"
     CDS             complement(10590..10850)
                     /GO_function="GO:0000036 - acyl carrier activity [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763164.1"
                     /locus_tag="I6J40_RS18780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18770"
                     /product="acyl carrier protein"
                     /protein_id="WP_030189428.1"
                     /sec_met_domain="PP-binding (E-value: 9.1e-06, bitscore:
                     24.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTTATDPIRDYIVQEFLAGEDAADLTDDFDLIESTVVNSLGLVR
                     LISWISETYGIPVDDIPLEPAAYRTLADIRRFVAEAAPAAV"
     gene            complement(10850..12295)
                     /locus_tag="I6J40_RS18785"
                     /old_locus_tag="I6J40_18775"
     CDS             complement(10850..12295)
                     /NRPS_PKS="Domain: AMP-binding (1-382). E-value: 6.1e-56.
                     Score: 181.7. Matches aSDomain:
                     nrpspksdomains_I6J40_RS18785_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452969.1"
                     /locus_tag="I6J40_RS18785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18775"
                     /product="AMP-binding protein"
                     /protein_id="WP_030189430.1"
                     /sec_met_domain="AMP-binding (E-value: 3.1e-56, bitscore:
                     189.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQEDPGRTALVAGGARVSYADLEQLARRTAADLDAVDRTDVRALC
                     VPAHKSPETIALLLAAWRRGINTLVPSPGLGAAALATLTAQAHGPLTAVAVPGGGAILS
                     REESDPALADGFSAPDPEESLLTLTTSGSTGVPKLVPIRTDAFDRFGDWATEKFGIDAG
                     ARSLSFSPLNFDLALLDVWTVLEAGGTVVLADAGASGDAAYLRALTSDNEVTLVQGVPL
                     LHRLLAADGARYPSVRTVVFTGESVSEQGLRDTFAACPAARFHNVFGCTETNDSFIEDI
                     DPDAFVHPLPIGAPIEGTEAVLLIEGPDGTEVLQGPGTGELLVHTPFQTCGYLEQARNA
                     HAFMPDPRGGGAMFYRTGDLVHRDADGRHFLQGRTDFQVKVRGVRTNTLEVETVLGAHP
                     DVVEACVVALPCPEAGTRLHAEVVRRDGTSLSSLGLRAYAAKHLPRHAVPSSVALAGTP
                     LPKTPTGKPDRNSIKKTRLNGDT"
     CDS_motif       complement(11120..11179)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_I6J40_RS18785_0002"
                     /evalue="5.80E-06"
                     /label="NRPS-A_a8"
                     /locus_tag="I6J40_RS18785"
                     /protein_end="392"
                     /protein_start="372"
                     /score="18.9"
                     /tool="antismash"
                     /translation="GRTDFQVKVRGVRTNTLEVE"
     aSDomain        complement(11150..12292)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_I6J40_RS18785_AMP-binding.1"
                     /evalue="6.10E-56"
                     /label="I6J40_RS18785_AMP-binding.1"
                     /locus_tag="I6J40_RS18785"
                     /protein_end="382"
                     /protein_start="1"
                     /score="181.7"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="QEDPGRTALVAGGARVSYADLEQLARRTAADLDAVDRTDVRALCV
                     PAHKSPETIALLLAAWRRGINTLVPSPGLGAAALATLTAQAHGPLTAVAVPGGGAILSR
                     EESDPALADGFSAPDPEESLLTLTTSGSTGVPKLVPIRTDAFDRFGDWATEKFGIDAGA
                     RSLSFSPLNFDLALLDVWTVLEAGGTVVLADAGASGDAAYLRALTSDNEVTLVQGVPLL
                     HRLLAADGARYPSVRTVVFTGESVSEQGLRDTFAACPAARFHNVFGCTETNDSFIEDID
                     PDAFVHPLPIGAPIEGTEAVLLIEGPDGTEVLQGPGTGELLVHTPFQTCGYLEQARNAH
                     AFMPDPRGGGAMFYRTGDLVHRDADGRHFLQGRTDFQVKVR"
     CDS_motif       complement(11870..11914)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_I6J40_RS18785_0001"
                     /evalue="4.90E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="I6J40_RS18785"
                     /protein_end="142"
                     /protein_start="127"
                     /score="13.1"
                     /tool="antismash"
                     /translation="LTTSGSTGVPKLVPI"
     misc_feature    12472..12486
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.73"
                     /tool="antismash"
     misc_feature    12483..12496
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.22"
                     /tool="antismash"
     misc_feature    complement(12514..12529)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     gene            complement(12632..13075)
                     /locus_tag="I6J40_RS18790"
                     /old_locus_tag="I6J40_18780"
     CDS             complement(12632..13075)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489542.1"
                     /locus_tag="I6J40_RS18790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18780"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_030189432.1"
                     /transl_table=11
                     /translation="MTINQQPRAASDQAMWEAVLGLHTFVERQLAHTLQRRYGVGLSEY
                     RALEALTQAEKGECRMQELADQIGLGQSSVTRLVGRLDSAGYAYKDSCADDKRGVFAVI
                     TEEGRKRYQEARATYADVLSSALNTASADDQLARAVQALRSAA"
     misc_feature    13170..13183
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.24"
                     /tool="antismash"
     misc_feature    complement(13170..13185)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.44"
                     /tool="antismash"
     misc_feature    13180..13193
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.38"
                     /tool="antismash"
     gene            complement(13200..14306)
                     /locus_tag="I6J40_RS18795"
                     /old_locus_tag="I6J40_18785"
     CDS             complement(13200..14306)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452971.1"
                     /locus_tag="I6J40_RS18795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18785"
                     /product="transposase"
                     /protein_id="WP_203702266.1"
                     /transl_table=11
                     /translation="MHETDLTASRPLGDLPRLLFGHLHRSDQQRWGHVYLQGLLRTHGK
                     KTVRRMAAVVTGSEAASTSLHQFVNASPWEWGPARGELARWAARSLPVRSWTLAPAVLP
                     KRGSHSAGVHQRFLPESGRTVNCQLGMGLFLDVGRAHVPVDWQLFLPPRFLRDEAERDR
                     AKIPATARPQPLWDQALSLVREMTSWSGGHTAPVVADLTSLCDTTPLTQRLQGSGHSFV
                     LAVPGQTALGGPEVPPGERESARAVLLRRGVRHPAGAGRQVISYETTVRGLRPDGERDL
                     GPGRLMAEWDAATGRPGRIWLTNITDRHPAELLELARSPRGARNAVRHMGEDFGLLDFE
                     GRSFPGWHRHMTLVSAAYACATLEGMTP"
     misc_feature    complement(14371..14384)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.3"
                     /tool="antismash"
     gene            14428..15072
                     /locus_tag="I6J40_RS18800"
                     /old_locus_tag="I6J40_18790"
     CDS             14428..15072
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018961774.1"
                     /locus_tag="I6J40_RS18800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_18790"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_030189436.1"
                     /transl_table=11
                     /translation="MQKRAARTRRTVLEAAADEFAERGYEGASLQGVAWRAATSIGALT
                     FHFRNKTALAEEVRTAGSARFTVCLEELPSCADPLRELRTLVGALVRLAHEDPFVRAAR
                     RLEADAPEDAPPLTEIWLPVLRTQLDRAHSARRLRPGVAPEDAVAMLSHLAEGAMAAHG
                     AARGAARHTAWEAAWDSVWDVVLHGLAADGPERGPEGTASPDLAGTCPSTG"
ORIGIN
        1 gtccgcgccc gcgaggccgg attcgacggg gtggaggtcc acggggccaa cggctatctg
       61 ctcgaccagt tcctgacccc gtacacgaac ctgcgcaccg acgagtacgg cggcggcctc
      121 ccggggcggc tgcggctcgt gcgggaggtg acggccgagg tcagagccgc ggccgggccc
      181 ggcttcacgg tcggcgtccg gctctcccag gggaagatca acgatcccgc gtggcgctgg
      241 ccgggcggcg agggcgaggc ggaggcggtc ttcacggcgg tcgccgaggc gggcgccgac
      301 tatctgcacc tggcgggcgg cgggcgcgac tggttccccg aggggcggac gctccccgcg
      361 atcgccaggg cggccaccgg gctgcctgtc atcgccaacg gcggcctgca ccgggatgcc
      421 gtggcgcggc gcgtcctgga ggagggccac gccgacctcc tggcggtcgg gacgggcgcg
      481 ctggccgatc ccgacctgcc gctcagggtg gcggcgggag aggcgctcac gccgttcgac
      541 gccgggatgc tggagcccct ggcgacgctg ggcaacgcgc ggacgtacgt gtccggcaac
      601 gcgtcgacgc acgcacccgc ttgacactgg ttacttgcac atgcaagatt aactccatca
      661 ggtcacgccg acggcggaga gctgtgaagc catttgcttg cgtgtgcaag caatcgcacg
      721 atgacgatgg tcgatgcagg gcaactcggg agacgaactg ccatgggtac acacgacagc
      781 gaactgatgg gctacgccca cgaggcgatc cggctcagcc gcgagcacgt cgcgcagggc
      841 ggcatcccgt tctccggagt ggtcgtgggc ggcgggcgca tcctgggcac cggtttcaac
      901 cgggtccgcg aggacaacga cccgaccgcg cacgccgagg tcgtcgccat ccgcgaggcc
      961 accacccggc acggcatccg cgccgtggcg ggggccaccc tgatcgcctc cggcgagccg
     1021 tgcgccctgt gctacatggc ggcccggttc gccggcatcg gccacatcgt ccacgccgcc
     1081 gaccgccgga ccgccgcacg gtacggcttc gactacacga gcacgtacac cctcttcgcc
     1141 gaggaccccg agcgctggcc cctcaaggtc accgcgctcc atctccccga ggcggagcag
     1201 ccgttccagg acttcctcac catggaccgc tcccggtact gacccgggcc cgcccgccac
     1261 cgcccgccgt cccgggcacg accgccgcac cccgtccccg acgtgacgcg gcccgcgcgg
     1321 cccccgcgcc gccgccggcc gagaggccga aacggcgccc cgcgcccccg ctccacgctc
     1381 caccccacct ctcgcaccca ccgcacggaa ggaccagcca tgcactggct ctacctcgcc
     1441 atcgccgtcg ccttcgagat cacggtcgcc atcgccgccg gaaaggccga gggcttcaag
     1501 aaccgcaagt ggaccgggat caccctggcc agcggcgccg ccgccacctt cttcctcagc
     1561 aaggcgctgc tgaccttcga cgtcggcgtc ggctacgccc tgtggacgtc cgtcgcgggc
     1621 gtcggcatca ccgtcctcgg cgcgctcttc ttcggccagc gcctgaacat caacaaggcg
     1681 atcggcatcc tcctcgtcat cggcggtgtc gtcggcctgc agctcagcgg ctccgcctga
     1741 gccccgcgca cccctcaccg gcgccgcaag cggcccaccc cgcacacccc tgacgacacc
     1801 tctgagagag gaaccacctc ccatgtccaa cgcggccaag agctccacca acgcctggct
     1861 ctcccttctg ctcgccggtg tcttcgagat cggctacgcg ctcgccgtcg gcggcagcga
     1921 aggcttcacg cagctgacct ggtccctggt cgccgtggtc ttcttcctgc tgaccctcta
     1981 cgccctcagc ctggccctcc gcacgatcga cgtcagcatc ggctacgcgg tctgggcggg
     2041 gatcggcgcg gtcggcgcgg cggtcttcgg cccgctgttc ttcgacgaga cgctcaccct
     2101 ggccaagggc atctggctgg ccgtcatcat cgtcggcgtc atctggctga agctggccga
     2161 ccgcacgaag cccgaggcgg tcgcggcccc ggccgccgag ccgcaggcca ccaaccggcc
     2221 cggcgccctc caggtctgac gcgccaccac cgcacgcggt gcaccgcgcg acagtacgcc
     2281 cccggcggct gcccccctcc acggaagggg agcggccgcc ggaggcgtcg gcgtcatacg
     2341 gcggtgcggc cgccgtccac ggcgagggcg gcccccgtgg tgaacgacga ggcgtccgag
     2401 cagagccagg cggcggtgcg ggcgatctcg accgggtcgg cgagacgccg ctggatcgcc
     2461 ttgccgccga agccggcgcg gacagcgggc ccgcccgcga ggacgccgtc catcatctcc
     2521 gtgtcggtgc tgcccaccac gagcgcgttg acccggatgc cgtactcgcc gtactcggcg
     2581 gcggccgccc gggtcaggcc gagcaccgcg tgcttcgccg ccacgtaggg cgcggacgcg
     2641 gaggtggcgc gcaccccggc gacgctggag gtgttgacga tcgcgccgcc gccggccgcg
     2701 agcagggcgg ggagctgggc gcgcaggcag ttccacaccc cgcgcaggtt gacgtcgagg
     2761 gtccggtcga actcgtcgtc ggacagctcg tgcaggagcg ttccggcgct cgcgtgcccg
     2821 gcgttgttga aggcgcagtc gaggcggccg aaccgttccg cggccacggc gaccgcgcgg
     2881 tccatgtccg cccggcgcgt gacgtcgccc ggtacggccg cggcccggcc gccgtccttc
     2941 ctgatccgct cggcggcctc ctcgatcctc tccgcccggc gggccaccag gacgactgcc
     3001 gcgccctcgt ccgcgaagag ccgggccgcg gcggcgccga tgccgctgga ggcgccggtg
     3061 atcagcgcga ccttgccggc cagcagcggt ccgcccggtc tcacgccgcc gtcccggccg
     3121 cgaccggagc ggcgtcccag aacgtgcccg aggcgaggtc gcggtcgatc cgccggacca
     3181 ggccgtcgtc gatctcgtcg aggccgccga tcagatggca tacgcccgac tcctccatga
     3241 gggcgcgctg gtggctgtgg acgaaggtgg aggggcggcc gacgaagccg gcgcccagct
     3301 cgcgggccac gtcggcgacc ttggccacgt cgtccacgaa gagggcctgc tcggggcgga
     3361 ggccgaacac gtcccgggtg atctccttca gtccggggcg gaagtcgttg gtgcagacgt
     3421 agccgggacc gtcgaacagc tcggcgtacc ggcccagatg acggtcgaag tggtccttgc
     3481 ccaggccgcc gtagcagacc gtgcgcaggc cggcggcgcg cagccggacg aggagttcct
     3541 ccgtgccggg cgtgatcgtg accgggtggc ggtcgaggtg ggcggcgcgg tcctcgaagt
     3601 aggcggcgag cgcctcctcg ccgctcatgg agccgccggt ggcggcggcg agcaggcggc
     3661 cggcgatgtg ctgcggctgg gagaagacgg atctctccag ctcggcggag tacgtaccgc
     3721 cccgggtggt gatgaagtgg tggatcaccg ggctgaacgt gtcgttgagc aggacaccgt
     3781 cgatgttcag ggcggccagg cgcagggtga gcatgcttct ccgtagggtt cgtcggaacg
     3841 ggcggagcgg ggcaggacag ccgtggtgct gggcatgccg aacgtgcggg gcatgtcggg
     3901 tgtgctggtc gtgctggtcg tgccgaaggg ccggccggtg ggaagcgccg gccgggtacg
     3961 gctgtgggcg gagcggcccg ggaggccgct ccggtcgggg cgtcggcgca ccgccgtgag
     4021 ccggggcgtc agcgcactgc cgtgagtctg ggcggcagtg cgttgccgca ggtcagggca
     4081 tcagcgcact gccgcgcgcg ggtgacgtct cggcgcgggt gatcacggac gggaccgcga
     4141 gccccggcag gatcaggttc cacatctgct gggtgcgttc gggaaggtcg ctgcggccgc
     4201 tgaccacgtt ggagtacatc tgcatgcccg tacacgcggc caccacgaac tcggcgacca
     4261 cgaccgggtc cacgcccggc tgcagctcgc cggactccag cgcctcgtgc aggcagccga
     4321 ccatgatccc gcggaagttc tcgtacgggt cggcgttgag ggtgccgttg atggacgcct
     4381 gctcgttggt cagccggacg ccggcccgca gcagcgggtc gcgctggagc tgccacgacc
     4441 agacgagggt gaggtcgatg agccgctgga ggccgccggc ggcgagcagt ggcaccaggg
     4501 tctccggctg gctgttcatc accgccttcg ccaggtcctc cttggagccg aagtggaagt
     4561 agagcgctcc cgccgtcacg ccggctcgct tgaggatgcg ggtgatgctg gctcccgcgt
     4621 agccgaactc gtcgaagacc tcggcggccg cggtcaggag ggcgcgacgg gtctgctccg
     4681 cccgctcctg tttcggttgt gccatgactc tcaccttccc aaaaagaatg ctccttatgt
     4741 tagccaggtg aacaggggga gaggcaagtc cgttatgtgg tatctgagtt agttttgcga
     4801 tcagatcgcc ccgagggaat atcgacggga ggatccccct tgacttgaga aaacaaaagg
     4861 aacattattt ttggagcggg gtcgcggaga gctgatcacc gatcgctctc cggtcacccc
     4921 gcgtcaaccg tcgaccttgg ggggccaagt tgtctgtttc tgttctgagc cgtggcgcga
     4981 gaccggtcca gcagtcgcag gtgcaccagt ggcaggtgca caagcttcgg ggcgaggaag
     5041 tcctgctcac gtcctggtta gaggcgagcc cgaaccagta cgtggtcacg gcgcgctggc
     5101 cgaagcgcca cgacttctac cggccggcgg ggggctcgta cgacccgctg ctgctcgcgg
     5161 agaccgtgcg ccagaccgtc ccgctgctct cccacgccgt gcacgacgtg ccgcgggagt
     5221 acaagcaggc gtgggaggac ttcagctttg ccgtcgagcc gctcgcctcg ctggtcacca
     5281 ccggcgagga cgaggtgcgc atggtggtgt cctgctcgga cgtgatccgg cgcggcgccc
     5341 ggttcgccgg gatgacgatg gacatcgacg tctacctggg caagcggctg atgggcatcg
     5401 cgcacacccg cttcaacaac cagcccgccg ccatctaccg ccgcctgcgc gggagttacg
     5461 cggacctcga cgcggcccag gagggctgcc tgccggtcgg gccgccggtg gagccgcacc
     5521 gcgtgggccg cgaccgggtg gaggacgtgg tgctctcccc ggcgctgggc ggcgagcgcc
     5581 ccggccggtg gctgctgcgg gtggacccct cccacccggt gctcttcgac catgtggtgg
     5641 accatgtgcc cggcatgctg ctgctggagg ccgcgcggca ggcggcgcac gacgtcgccg
     5701 gccccggcgc ggcggtcatc gggatgaaga cggacttcat ccggtacgtc gagttcgacg
     5761 cgcccaccgt cgtcgtggcc ggggagccgc tctccgccgg tacgggccgg cgccggatcc
     5821 ccctccgcgt cgagcagcac ggggtccagg tcttcaccgc cgaggtgacc gtccactgac
     5881 gccccccgcg gcatgacgcg gcgggcggcc cgtacctccc ggaagggaga cacggaccgc
     5941 ccgccgcgcg ctcgtgcccg ggccggctcc ggtgcgctcg tgccccggac cggtccggcc
     6001 gcgtgctcat gtccgggcca actccggcat cggttgcggc agttcctcgt acaccgtcac
     6061 cgatacgtgc accgaggccg ggaccgcgaa gctggcctcg tacccgggcg gggccagcag
     6121 ctgccgcacc cgccagccga gcggctggcc ggtgaggtcc agccggttca tgacggcggc
     6181 catcccctgc ccggtcgcct tggccatggc ctccttgcgg gtccacagcc gggcgaagtc
     6241 ccggaccagc tcgtcgcccg cgcggccgcc gagccactgc tgctccctgg ggtggaggaa
     6301 cggcaggtag ccggcgaccg actccggcgt cggccaggac tcgatgtcga tgccgatggt
     6361 ggcctcggcg accgcgatgg cgacgtggtc cccgctgtgc gacatcgaga actccagggg
     6421 ctccccggcc ggatccgggg ccgggctgcg gatggccggc cgcccgtgcg ccccgccgca
     6481 gccgggacag ggctgccgca ggaagccgac gctgccgggg gagcagccga gcgaggaccc
     6541 gagcacgctg cgcagcgccc agtgcgcgaa ggcgtaggac tcgcggccgg actgctgcgg
     6601 gatcgacgcc agccggctgc gctccttctc gtccagccac gggggcgcgg acccgtcggg
     6661 cgggaggcgc tgcccggccg gggcccggac gatccagatg ccgaccggat cgaggacctc
     6721 gcccgggggc gcgtcggacc acgtgaagtc gtgtgtcatg tcgatacggc tcctgatgcg
     6781 gcgggggccc ggccggcccc cgccacgggt cggatcaggc ggttgctgcg agcagcggcc
     6841 acgggccggt cgtggcgtac tgccccaggt cctcggaggc gaggtagcgc cggaaccccg
     6901 gtacctgctc cgcggggcgc agcgccccgc ggtacagcac catcccgcgc accccggtgt
     6961 cgtcgtcgtg ccagcacacg agcgggatgt ggtactccgg cgtgtcgcag gtctcctgca
     7021 cggcggcctg gtagccgtac gcgggcagca ggctcatcgc gccacgctcc cggcctcggg
     7081 gacgacctgg aacagggcgc cgtagtcggt gatccgcgcg gccgccccgc tgccgccggc
     7141 ggcgtgcagc gcgtgccaga acagcacctg gttcgcggtg aggacgggga agccgacggt
     7201 ctcctccagg ccggcgatgg tgccgacggc gcggatgccg ttgccggcga ccaggacggc
     7261 gtcggggcgg tgcggggcga cgtcggactc gatccacttc accagcgcct ccggggtgat
     7321 cagcttctgg ttgctgggga ggccgcaggg ggcgtggtgg acgacgtcga agccgcgctc
     7381 gccgaagtag gaggcgccca gggcggacag ctcgtcgtcg aaccacggcg ggttcaccag
     7441 cgcgatccgc tcggcgccca gggcgcggaa gccggccgcg gccgcgtgcg cggtgccggt
     7501 gagcgggatg ccgcgggtgc gctcggcgag ccgctcgaac agcttctgct cgccgtcggc
     7561 gcccaggacg taggaggagc tggtgaatcc gagggcgatg gagtccagcg gggaggcggc
     7621 cagcagctcg acggcgtcgt cgatgaaggg cgggtcgatg aaggcgcgca ccgggtcgtg
     7681 cgggatcttg gggtccatct ccccgccggg gcgcatcgcc ccgaagtgga cgcgggagcc
     7741 gtggatgaag acgtcgcgcg gcgccatcgc ctggagctcg gactcgggcc cgacgtccgc
     7801 gtgcggaacg acgacgccga cgcgggcgac cgcgccccag ccgtcctcac gggcctggaa
     7861 ctgcgtcatg gggtgcctct cctcttcttc tgccgttctt ctgcgggaat gccggtacgg
     7921 acctccgccg gcggcgccgg tacgaggggg cgtacgggta cggaaggggt gcgggtacgg
     7981 agggggggtg ccggcacgaa tggggtgccg gcaccaaggg gcgtgcgggg acgaacgggg
     8041 ggtgcgggga cgaaaggggg tgcgggcccg gcggcgcgtg cgggtccggg ggcggtgccg
     8101 ggcccggggt caggcggtcg cctcgtcggc gagcgagccg ccgctcacgg tgccggtgcc
     8161 gccctcgtcg aggaacttcg ggtgcttgcg gcgcatgttc tccatcacga acgcgtacgg
     8221 gacgtcgtcg aagtcgccgt ggtcggtgag gaactccatg ctctgccgcc cctgcgcgtc
     8281 gaaggggtgg tagaggctgt cggagcggcc gtcgaactcc agcggggtca tgccgtacag
     8341 ccggcacagc agcttgttga agaccggggt cacctggagc cagcggccgt cgaggtacac
     8401 ggtggccagg ccgtggaaga agacgtcccc gccgatgtag gagcgcagcc ggtcggaggc
     8461 caggtggttg cggacgtcgc tgtagtgcag ccgggccggg atgcccaccg cgcggcagca
     8521 ggcggcgtac agcacggact tgtgcaggca gaagcccttg ccgccggtga tgatgctgga
     8581 cgcgcgcagg ccctcgggcg acaggtcggc gccgtagacc tcgtagtgga tgccgtcgcg
     8641 gaccgcgtag tagagggtga cggcgagctc gaccggtccg gcggtctcgc ggtcggtccc
     8701 cttgagggct ttgtcgacga acgcctggac ggtcgggtgc cggtggtcga ggaagtcggt
     8761 ggcctcggtg aggcgggcga ggtcctcggc ggtcgaccgc tcggccgcgg cgtcggcggc
     8821 cggctgcgct gaggtggctt cggtcagggt ctcggtcatg gcgttgcctc tcgtcacagg
     8881 cccatcaggg cggcgatgca gttgcgctgg atctcgttgg tgccggagta gatggagccg
     8941 gcggtggcgt tgcgcagctc gcgctcgatg cccgtgtcgg tcaggtagcc gcgggccccg
     9001 aagatctgga tggcgtgccg ggcggtgagc tggttggcct cgctgaccac gatcttggtg
     9061 gcggccacgt ccagcgaggc gtccttgccc tgttcgacct tgcggccggt gtcgtagagc
     9121 cacttgcggg cggtctcgac cgcgatccgc atgtcggcga tcttgtgcgc gacggcctgg
     9181 ttcttgccga tcgaggcgcc gaactgctgc cgcgtggtgg cgtgttcgac gacgcgcttg
     9241 aggcggtggg tcatctcgcc gaggttgacc gcgaaggaga agaggacctc ccgcttcatc
     9301 acgtaatcca tgatcaggaa gccggcgccc tcgctgccga ggcggttcgc gacgggcagc
     9361 cggacgtccg tgagcgtcac ctcgctgatc ggggaggtgc gcagccccat cgtgtccagc
     9421 ggctcgccga ccgtcaggcc cggggtggag cgctcggcga ggaaggtgct cagcccgaac
     9481 gcgcccggct tgccggtgcg cacgtacagc aggaagaggt cggcgaccgg ggcgttggtg
     9541 atgaacagct tgccgccgtt gagcacgtag tcgtcgccgt cgcggacggc ggtggtgcgg
     9601 atgttcatca cgtccgagcc gtggcccggt tcggtgatgg cgtgcgcggt gatggcctcg
     9661 cccgaggtga cccgcggcag gtagcgctcg cgcagctccg cggagccgaa cgcctggagc
     9721 gggatgccga cgctgacgat ctgcgtggac gcggagaagt tgaggccggc gtcccggctg
     9781 gtgtggccga ggccctccag cgcggccatg gtctcggtga gcgtccggcc cgcgccgccg
     9841 tgctccgggg cgaaggggag ggtgaagagg ccggagcgct ggagcggctt ccacttctcg
     9901 tgcgggaact cgccggcgcg gtcccagcgg gtgacgtcgt cgttcagctc gtcggcgtac
     9961 ggcgcggtga ggccgtaggt gtccgtgtcg ttctctgctg ccatgggtgc tctgccttgc
    10021 ctttcgccgg tcgcgggcgc tgggggagcc gggtgcgggg ccgcgccgtc gcggccccgc
    10081 acccggcggt gcggatcagt agcgggagaa gccgtcgtcg gagagcgagt gcttctcgtc
    10141 gccgcgcagg gcggggctga agaccgagac gagcaggagg tcctcgtcgt cggaggcgat
    10201 caggtggtgg gcgtcgttct tgtccagggc gtagatggtc ccggtcttga tcttgtggcg
    10261 ggtgccgtcg gtggtgacga cctcgccgga gccgccgatg cagtagcagg cctccaggtg
    10321 gccgaggtac tgcagcgcgc tggaggtgcc ctggcggacc agcgtctcgc agacggtgaa
    10381 gcccatcccg tcctgctcgg tgaggaggcg gtggctggtg ccgctgcccc actcgaccgc
    10441 ggggacgtcg gagagagaac ggacgatcat gggagaactc ctgtcatcag ttgtgttccg
    10501 gacgtgtgtg caggtggtgc gggtacaact cgtgctgtgc cggtgcgccg cgtgtggggg
    10561 gtggttacgc ggagtgcggg ggcggtcggt cagaccgcgg ccggggcggc ctcggcgacg
    10621 aagcggcgga tgtccgcgag ggtgcggtag gcggccggct ccagcgggat gtcgtccacc
    10681 ggtatgccgt acgtctccga tatccaggag atgaggcgga ccaggccgag gctgttgacg
    10741 acggtgctct cgatcaggtc gaagtcgtcc gtcaggtccg cggcgtcctc gccggcgagg
    10801 aattcctgga cgatgtagtc gcggatcggg tcggtggcgg tggtgctcat cacgtgtctc
    10861 cgttcagtcg ggtcttcttg atcgagttgc ggtcgggctt gccggtcggg gtcttcggca
    10921 gcggggtgcc ggcgagcgcg accgagctgg gcacggcgtg gcgcggcagg tgcttcgccg
    10981 cgtacgcccg cagtcccagc gagctgaggg aggtcccgtc ccggcgcacc acctcggcgt
    11041 gcagccgggt gccggcctcg gggcagggca gggcgacgac acaggcctcg acgacgtccg
    11101 ggtgggcgcc gaggacggtc tcgacctcca gcgtgttggt gcgcacgccg cgcaccttga
    11161 cctggaagtc ggtacggccc tggaggaagt ggcgtccgtc ggcgtcgcgg tggaccaggt
    11221 cgccggtgcg gtagaacatg gcgccgccgc cgcgcgggtc gggcatgaac gcgtgtgcgt
    11281 tacgggcctg ttcgaggtat ccacaggtct ggaagggggt gtggaccagc agttcgccgg
    11341 tcccggggcc ctggaggacc tcggtgccgt ccggtccctc gatgagcagg accgcctcgg
    11401 tgccctcgat cggggcgccg atgggcagcg ggtgcacgaa ggcgtccggg tcgatgtcct
    11461 cgatgaagct gtcgttggtc tccgtgcagc cgaagacgtt gtggaagcgg gcggccgggc
    11521 aggcggcgaa cgtgtcgcgc agcccctgct cggagacgga ctccccggtg aacacgaccg
    11581 tgcggaccga ggggtagcgg gcgccgtccg cggcgaggag ccggtgcagc aggggcaccc
    11641 cctggaccag ggtcacctcg ttgtcgctgg tcagcgcccg caggtaggcg gcgtccccgc
    11701 tggccccggc gtcggcgagg accacggtcc cgccggcttc caggaccgtc cagacgtcga
    11761 ggagggccag gtcgaagttg agcggcgaga agctcaggct ccgggcgccg gcgtcgatcc
    11821 cgaacttctc cgtcgcccag tcgccgaagc ggtcgaaggc gtcggtgcgg atggggacga
    11881 gcttcggtac gccggtggag ccggaggtgg tgagggtgag gagcgactcc tcggggtccg
    11941 gggcgctgaa tccgtccgcg agcgcggggt cgctctcctc ccgcgacagg atcgcgccgc
    12001 ctccggggac cgcgacggcg gtcagcgggc cgtgcgcctg ggcggtgagc gtggccagcg
    12061 cggcggcgcc gagaccgggg gaggggacca gggtgttgat cccccgccgc caggccgcca
    12121 gcagcagcgc gatggtctcc ggcgacttgt gggccggtac gcacagggcg cggacgtccg
    12181 tgcggtcgac ggcgtccagg tccgcggcgg tccggcgggc cagctgctcc aggtccgcgt
    12241 agctgacccg ggcgccgccg gcgacgagcg cggtgcggcc gggatcttcc tgcacgtgtc
    12301 tgctgaatgc ctgcaagagg cgaggcgaag gcaacggtcc cccttggtgg ttgggcgatc
    12361 tggccggtgc gtccgttctg gctggttcga ccggccccac agatgtggct gtgctcgctg
    12421 gtgcggctgg atcggctggt gcggccggtc cggctacgag gaagaatcta cgggccttca
    12481 tttgcatggt caagcaaata ctttagccac tgttgtgacc tgggtcacag ctctgtcggt
    12541 tcgtgcgagg ccgctgtgga ggcccgaaca tgcaaagacg gcgggaggcc cgggtcaccc
    12601 cgggcgcctc ccgccgtctg gcgcgatctc gtcaggctgc gctgcgcagc gcttgcaccg
    12661 ccctggcgag ctggtcgtcg gcgctcgcgg tgttgagggc cgagctgagg acgtcggcgt
    12721 acgtggcacg ggcttcctgg tagcgcttgc ggccttcctc ggtgatgacg gcgaagacgc
    12781 cgcgcttgtc gtcggcgcag ctgtccttgt aggcgtatcc ggcgctgtcc agccgtccca
    12841 ccaggcgcgt caccgagctc tggcccagtc cgatctggtc cgcgagctcc tgcatccggc
    12901 actcgccctt ctcggcctgg gtgagcgcct ccagggcgcg gtactccgag aggccgacgc
    12961 cgtagcggcg ctgcagggtg tgtgcgagct ggcgctccac gaaggtgtgc aggccgagga
    13021 cggcctccca catggcctga tcggaggcgg cgcgtggctg ttggttgatc gtcatggaac
    13081 agctcccctt ctcgactgcg atgacaccaa gatacatgcc tgtgcaatca tctggggagg
    13141 gtcggggagt gcgggagggc tccctggccg atatttattt gtataggcaa gcaatggtgt
    13201 cagggggtca tgccctccag ggtggcgcag gcgtaggcgg cggacacgag ggtcatgtgc
    13261 cggtgccatc cggggaagga gcgtccttcg aagtcgagga ggccgaaatc ctcacccatg
    13321 tgccggaccg cgttccgcgc cccccggggc gagcgggcga gctccagcag ttcggccggg
    13381 tggcggtcgg tgatgttggt gagccagatc cgccccggcc ggccggtggc cgcgtcccac
    13441 tcggccatca gccgcccggg cccgagatcg cgctcgccgt ccggtcgcag tccgcgcacc
    13501 gtcgtctcgt acgagatgac ctgccggccc gcccccgccg ggtgccggac gccccggcgc
    13561 agcagtacgg cgcgcgccga ctcccgctcg ccgggcggga cttcgggccc gccgagggcg
    13621 gtctggcccg gaacggcgag gacgaaggag tgtccgctgc cctggagccg ctgcgtcagc
    13681 ggggtggtgt cgcacaggga ggtcagatcc gccaccaccg gggccgtgtg cccgccggac
    13741 caggaggtca tctcccgtac cagggacagc gcctggtccc acagcggctg gggccgggcc
    13801 gtcgccggga tcttcgcccg gtcgcgttcg gcctcgtcgc gcaggaagcg gggcggcagg
    13861 aagagctgcc agtcgaccgg gacgtgggcc cgtccgacgt cgaggaacag gcccatgccc
    13921 agctggcagt tgacggtgcg gcccgactcc ggcaggaagc gctggtgcac cccggccgag
    13981 tggctgcccc gtttgggcag gacggccggc gccagggtcc aggagcgcac gggcagtgag
    14041 cgggcggccc agcgggccaa ctcgccgcgc gccgggcccc attcccaggg gctggcgttg
    14101 acgaactggt gcagcgacgt cgaggccgcc tccgagccgg tcacgacggc cgccatgcgg
    14161 cgcacggtct tcttgccgtg cgtgcgcagc agcccctgca agtacacatg gccccaacgc
    14221 tgctggtcgg agcggtgcaa atggccgaac aggagccggg gcaggtcgcc cagcggccgg
    14281 ctcgccgtca agtcggtttc gtgcatcgtc gtcactgcgg gtccctccca ggtgagcgtc
    14341 gctcagcaga ctaacaaaca tagagaatgt tcgttatatt tctaggggga gagaacgact
    14401 acttgccgag gtccaaggag aaggcgcatg cagaaacggg cggccaggac gcgccggacc
    14461 gtgttggagg cggcggccga cgagttcgcc gagcgcggtt acgagggcgc ttccctccag
    14521 ggagtcgcct ggcgggccgc gacctccatc ggcgctctca ccttccactt ccggaacaag
    14581 accgccctgg ccgaggaggt gcgcacggcg ggatcggccc gcttcacggt ctgcctggaa
    14641 gaactcccgt cctgcgcgga cccgttgagg gagctgcgca ccctcgtcgg cgcgctggtc
    14701 cggctggcgc acgaggaccc cttcgtccgc gccgcccgca ggctggaggc cgacgcgccc
    14761 gaggacgcac cgccgttgac cgagatctgg ctgcccgtgc tccgcaccca gctcgaccgg
    14821 gcccacagcg cccgccggct ccgccccggg gtggcccccg aggacgcggt ggcgatgctg
    14881 tcgcacctcg cggagggggc gatggccgcc cacggcgcgg cgcggggcgc ggcccggcac
    14941 accgcgtggg aggcggcgtg ggactcggtg tgggacgtcg tcctgcacgg cctcgcggcg
    15001 gacggaccgg agcggggacc tgaggggacg gcctccccgg atctggccgg aacgtgcccg
    15061 agcaccgggt gaccggccga cgggggagcc gctcgccctc gcggtagggg tgcggagttc
    15121 aggcgcgccc gggtcaacag ggccgaagac tcgatgagtt ccccaccggg gccgcggcgc
    15181 cccgcgctcc gttcgcgggg gacaccccgg cgggcggacg gcgtccgccc cggaccgccc
    15241 cggtacgggg acggcccggg gcgcacgggc cgccgcgccc tcaggagccg tcccgcgccg
    15301 cccgctccac cgcgtccgcc acgtcgtcca gcacccgcgc cccgggggcc gccgtcagcc
    15361 ggccgcgcac ccacgccgcc ccggtcctgg cctgcggcca cgcctggaag gcgacgccgc
    15421 cgggcccctg gtggccgaag gccccggcgc ggaccgtagg ccagcggccc
//
