LOCUS       NZ_CP070260            11365 bp    DNA     linear   CON 30-MAR-2024
DEFINITION  Streptomyces californicus strain FDAARGOS_1210 chromosome.
ACCESSION   NZ_CP070260
VERSION     NZ_CP070260.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces californicus
  ORGANISM  Streptomyces californicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7919564)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7919564)
  AUTHORS   Sproer,C., Gronow,S., Severitt,S., Schroder,I., Tallon,L.,
            Sadzewicz,L., Zhao,X., Boylan,J., Ott,S., Bowen,H., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Lowell,S., Myers,T., Yan,Y.
            and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 624.55x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016906265.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 09:02:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,240
            CDSs (total)                      :: 7,147
            Genes (coding)                    :: 7,032
            CDSs (with protein)               :: 7,032
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 36 of 115
            Pseudo Genes (incomplete)         :: 97 of 115
            Pseudo Genes (internal stop)      :: 12 of 115
            Pseudo Genes (multiple problems)  :: 27 of 115
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:25:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1433955
            Orig. end                         :: 1445320
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070260.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11365
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="10"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11365
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11365
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6365](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6365
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(498..516)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.98"
                     /tool="antismash"
     misc_feature    501..519
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.96"
                     /tool="antismash"
     gene            540..1136
                     /locus_tag="I6J40_RS06235"
                     /old_locus_tag="I6J40_06235"
     CDS             540..1136
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938219.1"
                     /locus_tag="I6J40_RS06235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_06235"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_030187359.1"
                     /transl_table=11
                     /translation="MPKQVDHRERREAIARALWRVVDLRGVQHLSLREIAREAGISHGQ
                     LQHYFASRQALLTFAMDFAAEQTTQRISRSLEQLGDPPHPRDVLRLTLTEMLPLHADAR
                     ATSRMNAAYVLEALHDEEVLARTRDAMAQGRAQMEGLIRRAIADGHIDSTRDPATETDL
                     LLALTGFATLIELRVIAPQDVLKAVDQHLDRLFDQ"
     gene            complement(1415..1642)
                     /locus_tag="I6J40_RS06240"
                     /old_locus_tag="I6J40_06240"
     CDS             complement(1415..1642)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981773.1"
                     /locus_tag="I6J40_RS06240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_06240"
                     /product="hypothetical protein"
                     /protein_id="WP_078866762.1"
                     /transl_table=11
                     /translation="MSTGTGTGGGADDDGAYCVPAGPEAPAAPSGAAGGPPYAECVLCR
                     EPTEYPESTRGATLCPVCAWQEAGRSACSG"
     gene            complement(1657..2325)
                     /locus_tag="I6J40_RS35975"
     CDS             complement(1657..2325)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452202.1"
                     /locus_tag="I6J40_RS35975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_030187357.1"
                     /transl_table=11
                     /translation="MTALLRYQAALLARSQRWLAPLLLYAAVLAVGVQGGQPVLGSLGV
                     AAAALLPVTAWAVHLCVGQEPPAARAVVSAAVGARRVHLASLLTGTGCAAALAVVSTAV
                     VLLVSAPVTDDGAHEVARSSAALAGLLASLCCVLLGAMVGALSTRPVVERRGWSVAAAV
                     LGSLLVLVAAGSPARYAVTDLVTGSRTGTFHQPVLPLLAALAVAAPVAYFVCRTAARRG
                     "
     gene            complement(2322..3302)
                     /locus_tag="I6J40_RS35980"
     CDS             complement(2322..3302)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452203.1"
                     /locus_tag="I6J40_RS35980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_050494364.1"
                     /transl_table=11
                     /translation="MELRGVGHRYGLRGAWVLRGVDIALPARALVRVEGTNGAGKSTLL
                     RLLAGIDTPAEGRITGRRTRTAYVPERFSAALPLTATGYLVHLGRVHGLRTAEAKRHAG
                     EWLSRFGAAEHAHTPLAELSKGTSQKVAVAQALLAAPDLLVLDEAWTGLDAAARAELDR
                     AVAERVAAGATVVFVDHDPRRLAGAADLTLRAGDGGVGTVTAPVATGTAPGPRVLVVAV
                     GPRGASLPTPLPGDPDREREAGTPGSGADSVRLTVAASHSDALLKTLLTARPPWHIRQV
                     AAMGPPEPSRTVRPAGSAEGPASPGPGGSVRATGSAQPIERPATP"
     misc_feature    complement(3294..3296)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3335..4009)
                     /locus_tag="I6J40_RS06250"
                     /old_locus_tag="I6J40_06250"
     CDS             complement(3335..4009)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="I6J40_RS06250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_06250"
                     /product="AIM24 family protein"
                     /protein_id="WP_030114860.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHLAQPATAPGMTLQNAKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLALMAVRGQGEAWFAHEAANCFIVELDQGDSIT
                     INGRNVLCFDPTLSYEIKTVKGAGMVGGGLFNSVFTGYGKLGLMCEGTPIVIPVTSAQP
                     VYVDTDAVVGWSQQLTTSLHRSQSVGSMVRGGSGEAVQLMLQGEGFVIIRPSEARPEKT
                     SN"
     gene            4081..4809
                     /locus_tag="I6J40_RS06255"
                     /old_locus_tag="I6J40_06255"
     CDS             4081..4809
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072531.1"
                     /locus_tag="I6J40_RS06255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_06255"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_030114859.1"
                     /transl_table=11
                     /translation="MSSDDIPSPASESPFRSGPTDRDQAPQFVLPLVLHLEKTDPPART
                     DALRTAARAVLTILADERSLGDGAWAEAMRDWQDARIRKVVRRARGAEWRKAAALDGIT
                     VTGEDGAEVRVFPPIPLDGWPKELAKLQVSGTDLEDPEPPAAPDLSGPVLWLNPEVDMS
                     AGKTMAQVGHGAQLAWWELSGTERKAWREAGFPLSVATPGAARWRELTESGLPVVRDAG
                     FTEIAPGSCTVIADHPALRR"
     gene            5001..6365
                     /locus_tag="I6J40_RS06260"
                     /old_locus_tag="I6J40_06260"
     CDS             5001..6365
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100703.1"
                     /locus_tag="I6J40_RS06260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_06260"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_030187354.1"
                     /sec_met_domain="DUF692 (E-value: 6.6e-91, bitscore: 302.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELGIGIGWRPEIAHEVEALAGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVALGLGGADRPDPGRLAALAARAELLGAPLVTEHIAFVRAGGPLTAST
                     GLEAGHLLPVPRTWDALDVLCENVRIAQDSLPVPLALENIAALITWPDEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNLGQDPAKALDELPVEAIAYVHVAGGVEKNGVWHDTHA
                     HPVTAPVLEVLAELRSRVDPPGVLLERDDAFPPARELAGELDAIRATLRKAAPPAGSQA
                     PVESPARTVTVPGAPAPAPSISAPPAPAPSVSASSVSDAARDRTAVAQTALLSALVAGT
                     PAPAGFDPARLRMQSRSLAAKRADVVAKVAPELPEILGDGYRAAFLAYAGGRPMSGGYR
                     RDALDFAEHVLIAGGPADPVARRELTYWWRDRSGSRPPGRATRLIRAARAALVGR"
     gene            6371..7432
                     /locus_tag="I6J40_RS06265"
                     /old_locus_tag="I6J40_06265"
     CDS             6371..7432
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378555.1"
                     /locus_tag="I6J40_RS06265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_06265"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_030187352.1"
                     /transl_table=11
                     /translation="MNVLALVLNIAALVSAVLLVTTAVSAGSRRPSAGPFPGVHDLYEA
                     AFLNGGPARVVDTALTALYADGRVLIGGPGIISVQRPQADDPVERAVLHELSAAPSGAL
                     HVIREAAMRHPAVQEIGDGLAQRGLLVPPAANRGVWRWGLVQGVSCLVAFPVSIVLTVL
                     QYATHGASFDLPAPFVVKMLPAIVVGGISGLVVASVAARRITGAGRRAAQGFGAAHAQA
                     ADPAHLVAVRGLRALPDPALRAQLIAAARLGAPRRRRSSGHTYGTSTTGATGVAATTVW
                     CAASGPGASSCGGSSGGGSGSGCGGGSGCSSGSGCGGGSGSSCGGGGSSCGGGGGGSGC
                     GGGGGGGCGGGGS"
     gene            7705..9267
                     /locus_tag="I6J40_RS06270"
                     /old_locus_tag="I6J40_06270"
     CDS             7705..9267
                     /NRPS_PKS="Domain: Abhydrolase_1 (101-476). E-value:
                     5.2e-17. Score: 54.2. Matches aSDomain:
                     nrpspksdomains_I6J40_RS06270_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="I6J40_RS06270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_06270"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_030187350.1"
                     /sec_met_domain="PF00561 (E-value: 1.6e-22, bitscore: 79.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAALYTTVGSLVLSALAVAPAAAREGRGPAEARGTAIAAARAA
                     SAGIDFAACPEEEMLPDSLKCGTVKVPLDYAEPDGRQLELTVSRTPATGPAEGRQGAFV
                     YNPGGPGASSITFPMAGELPEWKEIAEAYDLVGYAPRGVNGSSSPLSCQDPSAYTKGPT
                     DAPVHPTQADKERRIARARAYARGCAEHAGDTLRHYTSLNNARDLDVLRAALGEPRLTF
                     MGASYGTYFGALYATLFPTHVRRMVFDSVVDPDPEQIWYRSNMGQSVAFERRWEDFRRW
                     VAKHDDVYRLGTTPEAVGKHYDAVRAELAVKPAGGKVGPGQLHAAFLSAGYYDDYWAMR
                     ATALSEYVRGNPEPLVAQASPRAVAAKDNENAQAVYTAVECNDAPWPEDFAVWDRDHTE
                     LARVAPFEAWDNAFTNLPCAFWPAPRQRPLEVGTRWGVLPPVLILAAERDAATPYRGAL
                     ELRRRLPGSSLVTERGAGTHGVGGAGNTCVDEHLRRYLLTGEVPGRGADCAARPEPNAV
                     SLD"
     aSDomain        8008..9132
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_I6J40_RS06270_Abhydrolase_1.1"
                     /evalue="5.20E-17"
                     /label="I6J40_RS06270_Abhydrolase_1.1"
                     /locus_tag="I6J40_RS06270"
                     /protein_end="476"
                     /protein_start="101"
                     /score="54.2"
                     /tool="antismash"
                     /translation="AFVYNPGGPGASSITFPMAGELPEWKEIAEAYDLVGYAPRGVNGS
                     SSPLSCQDPSAYTKGPTDAPVHPTQADKERRIARARAYARGCAEHAGDTLRHYTSLNNA
                     RDLDVLRAALGEPRLTFMGASYGTYFGALYATLFPTHVRRMVFDSVVDPDPEQIWYRSN
                     MGQSVAFERRWEDFRRWVAKHDDVYRLGTTPEAVGKHYDAVRAELAVKPAGGKVGPGQL
                     HAAFLSAGYYDDYWAMRATALSEYVRGNPEPLVAQASPRAVAAKDNENAQAVYTAVECN
                     DAPWPEDFAVWDRDHTELARVAPFEAWDNAFTNLPCAFWPAPRQRPLEVGTRWGVLPPV
                     LILAAERDAATPYRGALELRRRLPGSSLVTERGAG"
     gene            complement(9556..10284)
                     /gene="hemQ"
                     /locus_tag="I6J40_RS06275"
                     /old_locus_tag="I6J40_06275"
     CDS             complement(9556..10284)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="I6J40_RS06275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="I6J40_06275"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_030187348.1"
                     /transl_table=11
                     /translation="MSAPETATSSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRA
                     GYADEVQELFDQLAAEDVTVRGTYDLSGLRADADIMIWWHAETSDQLQDAYNLFRRTKL
                     GRALEPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARLHVRE
                     EVPFYTGRRKSVADLVAGLA"
     misc_feature    10764..10780
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.3"
                     /tool="antismash"
     misc_feature    11232..11245
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 19.64"
                     /tool="antismash"
ORIGIN
        1 gcggttggcc ggagcgccgt cgagcagggc gaacccttcc gcgcccattc ggcagttgac
       61 actcacgacg acgagccccg ccccggtcag cacggcgggg tcgtacatcg gatcacccga
      121 gtcccccgcc atatagcctc cgcagatgat ccacaccagc accggcaggc ccccaccgcc
      181 ggggtccggc gtggacacat cgagcgtcag ccagcccgtg ccgtcggacg gatccgcccg
      241 ggtcggcccg gactgcggtg cggcgggtcc gaatgcgacc gccggccgga cgccctccca
      301 gggacgcgcg gggacaggcg cggcgaagcg gagcgcgccc acgggcggct gagcgtaggg
      361 gataccgcgg aacaccgcgt gcccctgtcg ccggcggccc tccacatggc cctcggcggt
      421 gcgcactctt ggctgctcag gcacagctcg tcctctccgg ccggtgtccg taggttcccc
      481 agggtttatc ggtcacctgt attatgtcaa ctgtatgaga acgaggagcg gggcggacca
      541 tgccgaaaca agtggatcac cgcgaacgac gcgaagcgat cgcccgagca ctgtggagag
      601 tggtggatct gcgcggagtg cagcacctga gcctgcgcga gatcgcacgg gaggcaggca
      661 tctcccatgg acagctgcag cactacttcg cctctcggca ggccctgctc acgttcgcca
      721 tggacttcgc cgccgagcag acgacgcagc gcatcagccg aagcctggaa cagctcggtg
      781 atccgccgca cccccgcgac gtactccgac tgacgctcac ggaaatgctt ccgctccacg
      841 ccgacgcccg tgcgaccagc cggatgaacg ccgcgtacgt cctggaggcg ctgcacgacg
      901 aagaggtcct cgcacgcacg cgtgacgcca tggcccaggg acgcgctcag atggaggggc
      961 tgattcgccg ggcgatcgcg gacgggcaca tcgactccac ccgcgacccg gcgaccgaga
     1021 ccgaccttct tctcgctctc accggcttcg ccaccctgat cgaactgcgc gtgatcgctc
     1081 cccaggacgt gctcaaggcc gtggaccagc acctggacag gctgttcgac cagtagcgcc
     1141 ttgtcggatg gggtcgcggg gcggccatgg ggcggggaac cggtcggcgg acatcccgta
     1201 cgtgaccggc ccgctcgcgc cttccggtca ccgggcccgc gggacgtgtg cccggggcgc
     1261 ccatgaccgc gccccgcgac gggttcgcgg tactcgccgc gcaccctggc gacgcgcccg
     1321 gcggggtggt tggcgtgctg atgggtaccc ggcggaccag gggcggacag gcgggccagg
     1381 tgtcgtggcc cggtcaggac gtcccggaca cgccctaacc ggaacaggcc gagcggccgg
     1441 cctcctgcca ggcgcagacc gggcagagcg tggcgccccg ggtcgactcc gggtactcgg
     1501 tcggctcgcg gcacagcacg cactcggcgt acggagggcc gccggccgcc ccgctaggcg
     1561 cggccggtgc ttcgggtccg gcaggcacgc agtacgcacc gtcgtcgtcc gcgccgccgc
     1621 ccgtgcccgt acccgtactc atgcccacga gcctactcac ccgcgccggg cggctgttcg
     1681 gcacacgaag tacgccacgg gagcggcgac ggccagcgcg gcgagcagcg gcaggacggg
     1741 ctgatgaaac gtcccggtcc gcgatccggt gaccagatcc gtgaccgcgt accgcgcggg
     1801 ggagcccgcg gccaccagca cgaggagcga cccgagtacg gccgccgcga cggaccagcc
     1861 ccggcgttcc accacggggc gtgtgctcag agctcccacc atcgcgccca gcagtacaca
     1921 gcagagggag gcgagcagtc ccgcgagcgc ggcggaggag cgggcgacct cgtgggcgcc
     1981 gtcgtcggtg acgggggcgc tgacgagaag gacgacggcc gtactcacca ccgcgagcgc
     2041 cgccgcacag cccgtgccgg tgagcaggga cgccagatgg actcgccggg ccccgacggc
     2101 cgccgagacg acggcccggg cggcgggcgg ttcctgaccg acgcagaggt gaacggccca
     2161 ggcggtgacg ggcagcaggg cggctgccgc gacgccgagc gagcccagca cgggctgccc
     2221 gccctgcaca ccgacggcga ggacggcggc gtacagcaga aggggcgcga gccagcgctg
     2281 ggagcgggcc agcagggcgg cctggtagcg gagaagcgcg gtcacggtgt cgcgggcctc
     2341 tcgatcggct gagcggagcc ggtcgcccga acgctcccgc ccggcccggg tgacgccggc
     2401 ccctcggcac tcccggcagg tcggacggtc cgggacggct ccggcggccc catcgcggcg
     2461 acctgccgga tgtgccaggg cggacgggcc gtcaacagcg tcttcaggag cgcgtccgag
     2521 tgggacgcgg cgaccgtgag gcgtacggag tcggcgccgg agcccggagt gccggcttcc
     2581 cgttcccggt ccgggtcgcc gggcaacggc gtcggcaggg acgccccgcg cggccccacg
     2641 gccacgacca ggacgcgggg gcccggggcg gtaccggtcg cgaccggcgc agtcaccgta
     2701 cccaccccgc cgtccccggc ccgcagcgtg aggtcggccg ccccggccag acgccgggga
     2761 tcgtggtcga cgaagacgac ggtggcgccg gccgcgacgc gctcggccac ggcacggtcg
     2821 agttccgcgc gggcggccgc gtcgagcccc gtccacgcct cgtccagcac cagcaggtcg
     2881 ggggcggcga gcagcgcctg ggccacggcc accttctggc tggtgccctt ggagagttcg
     2941 gcgagtgggg tgtgggcgtg ctcggccgcc ccgaaccggc tcagccactc ccccgcgtgc
     3001 cgcttcgcct cggcggtccg caggccgtgg acgcggccga ggtggacgag gtagccggtg
     3061 gccgtcaggg gcagcgccgc cgagaagcgt tcggggacgt acgccgtgcg ggtccggcgg
     3121 cccgtgatcc gcccctccgc cggggtgtcg atcccggcca gcaaccgcag cagggtcgac
     3181 ttcccggccc cgttggtgcc ctcgacacgg acgagggcgc gcgcgggcag cgcgatgtcc
     3241 accccgcgca gcacccaggc gccccggagg ccgtaccggt gccccactcc ccgtaactcc
     3301 acgccccgta cccccggacc ccttcggtgc ggcctcagtt ggaggtcttc tccggcctgg
     3361 cctcgctcgg gcggatgatg acgaagccct cgccctggag catcagctgg accgcctcgc
     3421 cggagccgcc gcgcaccatc gagccgacgc tctgcgagcg gtgcaggctc gtcgtgagct
     3481 gctggctcca gccgacgacc gcgtccgtgt ccacgtacac cggctgggcg gaggtcacgg
     3541 ggatcacgat cggggtgccc tcgcacatca ggccgagctt gccgtagccc gtgaagacgc
     3601 tgttgaagag gccgccgccg accatgccgg cgcccttcac cgtcttgatc tcgtaggaga
     3661 gcgtggggtc gaagcacagg acgttgcggc cgttgatggt gatggagtcg ccctggtcca
     3721 gctccacgat gaagcagttc gccgcctcgt gcgcgaacca cgcctcgccc tggccccgga
     3781 cggccatgag cgcgaggccc tcaccggtga ccgcgcgctt gagcatgccg ccgatgccct
     3841 ggcccttgcg ctcgaactgg aggttgccgc ggaaggcgat catcgaaccc tggcgggcgt
     3901 gcatctcccc gttgacggcg tacttgatcg acttggcgtt ctgcagggtc atgccggggg
     3961 cggtggccgg ctgggccaga tgctcgctgg aaaagagatc gctcttcatg cggagcatcc
     4021 tcacccggac cacgccgttc cgccaagcag gtgaccgcgc ccgcctggca gactggggcg
     4081 gtgagcagcg acgacatccc ctcccccgcc tccgagagcc ccttccgcag cggcccgacc
     4141 gaccgcgacc aggcgccgca gttcgtcctg ccgctggtgc tccacctgga gaagacggac
     4201 ccgcccgccc gtaccgacgc cctgcggacc gccgcccgcg cggtgctgac gatcctcgcc
     4261 gacgagcggt ccctcggcga cggggcgtgg gcggaggcga tgcgggactg gcaggacgcc
     4321 cgcatccgca aggtcgtgcg ccgggcgcgc ggcgcggagt ggcgcaaggc cgcggcgctg
     4381 gacggcatca cggtgacggg cgaggacggc gcggaggtac gggtgttccc gccgatcccg
     4441 ctggacggct ggcccaagga gctggcgaag ctccaggtgt cgggcaccga tctggaggac
     4501 ccggagccgc ccgccgcgcc cgacctgtcc ggtccggtgc tctggctgaa ccccgaggtc
     4561 gacatgtcgg cgggcaagac gatggcccag gtcgggcacg gggcgcagct cgcctggtgg
     4621 gaactgtccg ggaccgagcg caaggcgtgg cgcgaggccg ggttcccgct ctccgtggcg
     4681 acccccgggg cggcacgctg gcgggagctg acggagagcg ggctgccggt cgtgcgcgac
     4741 gccgggttca ccgagatcgc cccgggctcc tgcaccgtga tcgccgacca tccggcgctg
     4801 cgccgctgag cgcctcctcc gccacctccc cggaggccgc cgatgcccgc ggcgggcggg
     4861 ccgggggcgg cgggcgcggc gggcgcggcg ggccgggaac ctcaaatcct gctctgaacg
     4921 tcccactgct cgggttcatc gccgcgtgac ggggccatga ccccggaaca gaacgaggtg
     4981 agcaggggga cgggggcacc atggaactgg gcatcggcat cggctggcgg ccggagatcg
     5041 cgcacgaggt ggaggcgctg gccgggatcg actgggtgga ggcggtcgcg gagaacctct
     5101 gcgccgacca tctgccggac tccctggtac ggctccggga gcgcggcgtc accgtcgtgc
     5161 cgcacggggt ggcactcggc ctcggcggcg cggaccggcc cgaccccggc cgcctcgccg
     5221 ccctggcggc gcgggccgag ttgctgggcg cgccgctggt gaccgagcac atcgcgttcg
     5281 tacgggccgg ggggccgctg accgcgtcga cggggctgga ggcggggcac ctgctgccgg
     5341 taccgcgcac ctgggacgcg ctcgacgtgc tgtgcgagaa cgtgcggatc gcgcaggact
     5401 cgctgccggt gccgctcgcc ctggagaaca tcgccgcgct gatcacctgg cccgacgagg
     5461 agctgaccga agggcagttc ctggccgagc tggtcgagcg gaccggggtg cggctgctga
     5521 tcgacgtggc caatctgcac accaaccacg tcaacctcgg ccaggacccg gcgaaggcgc
     5581 tggacgagct gccggtggag gccatcgcgt acgtccacgt ggcgggcggg gtcgagaaga
     5641 acggcgtctg gcacgacacg cacgcccacc cggtgaccgc tcccgtgctg gaggtgctgg
     5701 cggagctgcg gtcccgggtc gatccgccgg gcgtgctgct ggagcgggac gacgcgttcc
     5761 cgccggcgcg ggagctggcg ggcgaactgg acgcgatccg ggcgacgctg cggaaggcgg
     5821 ctcccccggc ggggtctcag gctccggtgg aatccccggc ccggacggtc accgttcccg
     5881 gcgcgcccgc ccccgcgccg tccatctccg cgccgcccgc ccccgcgccc tccgtctccg
     5941 cgtcgtccgt ctccgacgcc gcccgtgacc gtacggccgt cgcacagacc gcgctgctct
     6001 ccgcgctggt cgccgggacg cccgcccccg ccggcttcga ccccgcgcgg ctgcggatgc
     6061 agagcaggtc gctggcggcc aagcgcgcgg acgtcgtcgc caaggtcgcc ccggagctgc
     6121 cggagatcct cggcgacggc taccgcgcgg cgttcctcgc gtacgcggga ggccggccga
     6181 tgtccggcgg ctaccggcgc gacgccctgg acttcgccga gcacgtgctc atcgcgggcg
     6241 gccccgccga tccggtggcg cggcgcgagc tgacgtactg gtggcgggac cgctcgggtt
     6301 cccgcccgcc gggccgggcc acccggctga tccgtgcggc gcgcgccgcc ctcgtgggga
     6361 ggtgaacgcc gtgaacgttc tcgctctggt gctgaacatc gcggctctcg tctcggcggt
     6421 cctcctcgtc acgaccgccg tctccgccgg gtcccgccgc ccgtcggccg ggcccttccc
     6481 cggcgtccac gacctctacg aggccgcgtt cctgaacggc ggaccggcca gggtggtgga
     6541 caccgcgctc accgccctgt acgcggacgg ccgcgtgctc atcggcggcc ccggcatcat
     6601 ctccgtacag cgtccgcagg cggacgaccc ggtggaacgc gccgtgctcc acgagctgtc
     6661 ggccgctccg agcggcgcgc tgcacgtcat acgggaggcg gcgatgcgtc acccggcggt
     6721 gcaggagatc ggcgacgggc tggcgcagcg cgggctgctc gtcccgcccg ccgcgaaccg
     6781 cggcgtatgg cgctgggggc tggtgcaggg cgtgagctgt ctggtcgcgt tcccggtctc
     6841 catcgtgctg acggtcctcc agtacgcgac gcacggcgcg tccttcgacc tgccggcccc
     6901 gttcgtcgtc aagatgctgc cggcgatcgt ggtcggcggg atcagcggtc tcgtcgtcgc
     6961 ctcggtcgcg gcgaggcgga tcaccggggc gggccgccgg gccgcgcagg ggttcggggc
     7021 cgcccacgcc caggcggccg acccggccca cctggtcgcc gtgcgggggc tgcgcgcgct
     7081 gccggacccg gcgctccggg cccagctgat cgcggcggca cggctgggcg ccccgcgccg
     7141 ccgccgctcc tccggccaca cctacgggac ctccacgacg ggggccaccg gcgtagccgc
     7201 cacgacggtc tggtgcgccg cctccggccc cggcgcatcg agctgcggcg ggtccagcgg
     7261 gggcggttcc gggtcgggat gcggcggcgg atccgggtgc tcctcgggct ccgggtgcgg
     7321 cggtggctcg gggtcgagct gcggaggcgg cggatcgagc tgcggagggg gaggcggcgg
     7381 ctccggctgc gggggcggag gcggtggcgg gtgcggaggc ggcggctcct gaaccggacg
     7441 cctcggatac ggcgcggctc ctgagccggg cgcggcgaag acagccgtgg cccctgaagc
     7501 ggacgcggcg aagacggctc ccggaaagga cccgagcggc ctgaaacagc cctgtacgta
     7561 aggtcgttga ccacccatag gggtggttca cggggacatg cgacgacagt gctggacatc
     7621 gccgcccgcc cgcccgacca tccctctccg catccgcgga gaggcaccgc cgtccctctc
     7681 ctcatccgca gagaggtacc gcgcgtgaga acagctgcgc tctacaccac cgtcggctcc
     7741 ctggtcctgt ccgctctggc cgtcgcgccg gcggccgccc gggagggccg gggccccgcc
     7801 gaggcccgag gcaccgcgat cgccgccgcc cgcgccgcgt ccgccgggat cgacttcgcc
     7861 gcctgccccg aggaggagat gctcccggac tccctgaagt gcggcacggt gaaagtgccg
     7921 ctcgactacg ccgaaccgga cgggcggcag ctcgaactga ccgtcagccg cacccccgcc
     7981 accggtccgg ccgagggccg gcagggcgcg ttcgtctaca acccgggcgg ccccggcgcg
     8041 tccagcatca ccttcccgat ggccggggaa ctgccggagt ggaaggagat cgcggaggcg
     8101 tacgacctgg tcggctacgc cccgcgcggg gtgaacgggt cctcctcgcc gctcagttgc
     8161 caggacccgt ccgcgtacac gaagggcccg accgacgccc ccgtccaccc cacacaggcg
     8221 gacaaggagc ggcggatcgc ccgtgcgcgg gcctacgcgc gaggctgcgc cgagcacgcg
     8281 ggcgacaccc tgcgccacta cacctcgctg aacaacgccc gggacctgga cgtgctgcgg
     8341 gccgcgctgg gcgagccgcg gctcacgttc atgggggcgt cctacgggac gtacttcggc
     8401 gcgctgtacg cgaccctgtt ccccacccac gtacggcgca tggtcttcga ctcggtggtc
     8461 gacccggacc ccgagcagat ctggtaccgc tccaacatgg gccagtcggt ggccttcgag
     8521 cggcggtggg aggacttccg gcgctgggtg gcgaagcacg acgacgtgta ccgcctgggc
     8581 accacccccg aagcggtggg gaagcactac gacgcggtgc gcgccgagtt ggcggtgaag
     8641 ccggcgggcg gcaaggtcgg gccggggcag ttgcacgcgg cgttcctgtc ggccggctac
     8701 tacgacgact actgggcgat gcgggcgacg gcgctgtccg agtacgtccg gggcaacccg
     8761 gaaccgctgg tggcgcaggc gtccccgcgc gcggtggcgg ccaaggacaa cgagaacgcg
     8821 caggcggtgt acacggccgt cgagtgcaac gacgcgccgt ggcccgagga cttcgccgtc
     8881 tgggaccgcg accacacgga gctggcgcgc gtcgcgccct tcgaggcgtg ggacaacgcg
     8941 ttcaccaatc tgccgtgcgc gttctggccg gcgccccggc agcgcccgct ggaggtcggg
     9001 acgcggtggg gcgtgctgcc gccggtgctg atcctggcgg cggagcggga cgcggcgacc
     9061 ccgtaccggg gggcgttgga gctgcggagg cggctgcccg gctcgtcgct ggtgaccgag
     9121 cggggcgcgg ggacgcacgg ggtcggcggg gccgggaaca cgtgcgtgga cgagcatctg
     9181 cgccggtacc tgctgaccgg cgaggtgccg gggcggggcg cggactgcgc cgcccgaccc
     9241 gagcccaacg cggtgtcgct ggactgacgc cgaaggcgcg cgccgtacgg gcacgcggac
     9301 gcggcagcgc acgggcgtac gggcgcaccg acgcaccgac gcccggtcac acgagcacag
     9361 cagcgcaccg gcgtacaggc acgcggacgc ggcggcgtac gggcgtaccc gcgtacggcg
     9421 agggggccgc cgcaccttca cggtgcggcg gcccccgaag ccgaacccgg tgtcgctgga
     9481 acggcgggcg gcacggtcgg tgcccggtgc ccgcccttct gatcttccgg gctggggtgg
     9541 tgcgggactc gctggctatg cgagcccggc caccaggtct gcgacgctct tcctgcggcc
     9601 cgtgtagaac gggacctcct cgcgcacgtg gagacgtgcc tcggaggcgc gcaggtggcg
     9661 catcaggtcg acgatgcggt acagctcgtc ggcctcgaag gcgaggatcc actcgtagtc
     9721 gccgagcgag aacgaggcga cggtgttggc gcggacgtcc gggtagcccc gggccatctt
     9781 gccgtggtcc gcgagcatgc ggcggcggtc ctcgtcgggc agcaggtacc agtcgtagga
     9841 ccggacgaag gggtagacgc tgatgtagtc gcgcggcgtc tcgtcggcga ggaaggccgg
     9901 gatgtgcgac ttgttgaact cggcgggccg gtgcagggcc atgttggacc agaccggttc
     9961 gagagcgcgt ccgagcttgg tgcggcggaa gaggttgtac gcgtcctgga gctggtccga
    10021 ggtctcggcg tgccaccaga tcatgatgtc cgcgtcggcc cgcagacccg aaaggtcgta
    10081 ggtgccgcgg acggtgacgt cctcggcggc gagctggtcg aacagctcct ggacctcgtc
    10141 ggcgtatccg gcgcggtcca gggggagcac gtcgcgcagc ttgaagaccg accacagggt
    10201 gtagcggatg acctcgttga ggtccttggc cttcttgccg gcgttcggac tcttgcttga
    10261 tgtcgcagtc tcaggagcac tcatacggct attgtcccgc ctcgctcccc gtgccccgaa
    10321 cccgggtcgg cgtggcgatg atctcccccg cgatctcccc gtcgatccgc gccgggttca
    10381 tgcccgtgct tccgcccggg ttctccgcca ggtcgcggag gatctcgtcg gccgcccggt
    10441 gcgcgctcgc gacgcaggcc gggatgccga ccccgtcgta gaccgcgccg cagacccgca
    10501 gcgcgggcag tccggcgacc gcgtcgcgga tgcgggcgac ccgggcgagg tggccgaccg
    10561 ggtactgcgg caggccgccg atccagcggg tgacctcggt gtccacgggg cgggcggtga
    10621 ggccggtggc ggcggcgagg tcgcgcaggg agacggcgac gagttcgccg tcctcgcggt
    10681 ggatgtggtc ctcctcgccg tagcggccga cggaggtgcg gagcacgaac aggtcgggcg
    10741 cggcgtcggc gacccactgc cacttgttgc tggagaacgt ggacgccttg atggtgtgcc
    10801 cgtcgaccgg cggcacgagg aagccggagc gcccgcgcag cgccgtgtgc gcctcgatgt
    10861 cggcgcgccg gaaggccagg gtcaccaggg ccatcgaggc gtactcgacg ccggacagtt
    10921 cggcggaggc ggccggggac tcggcggcca gcagggtggc ggcggaccag gcgggggcgg
    10981 ccaggacgac gccgtcggcg gcgatggtcc gggcgtcggt gcggacggcc cagccggatt
    11041 ccgtacgggt caggccgagg acgggggtgg cggtgaggat ctcgccgccg ccggcccgta
    11101 cggcgtcgcc cacggcggcc gggagcgtgc cgatgccgcc cgcgatgccc tggaaaacgg
    11161 ggccggtctg ctgccgggcc gcggcgcgtt cctggatgcg gcggacggcg tccagcagcg
    11221 ggccgccctc ctggaccgcg tcgaagagct ggggcacggc ggcgcgcatc gagatccggt
    11281 aggcgtcgcc cgcgtatacc ccgccgagca ggggctccac cagccggtcg acgacctcgc
    11341 ggccgagccg gtcggcgacg tacgc
//
