GCF_016905205.1 - 2 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_JAFFHJ010000003.1 (Streptococcus suis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 9.1	T3PKS,terpene-precursor	1	34,682

NZ_JAFFHJ010000004.1 (Streptococcus suis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 10.1	lanthipeptide-class-i	124,732	150,743	Medium	suicin 90-1330	ribosomal:RiPP

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 9.1	T3PKS,terpene-precursor	1	34,682
Region 10.1	lanthipeptide-class-i	124,732	150,743	Medium	suicin 90-1330	ribosomal:RiPP

No secondary metabolite regions were found in these records:: NZ_JAFFHJ010000001.1 (Streptococcus suis); NZ_JAFFHJ010000010.1 (Streptococcus suis); NZ_JAFFHJ010000011.1 (Streptococcus suis); NZ_JAFFHJ010000012.1 (Streptococcus suis); NZ_JAFFHJ010000013.1 (Streptococcus suis); NZ_JAFFHJ010000014.1 (Streptococcus suis); NZ_JAFFHJ010000015.1 (Streptococcus suis); NZ_JAFFHJ010000002.1 (Streptococcus suis); NZ_JAFFHJ010000005.1 (Streptococcus suis); NZ_JAFFHJ010000006.1 (Streptococcus suis); NZ_JAFFHJ010000007.1 (Streptococcus suis); NZ_JAFFHJ010000008.1 (Streptococcus suis); NZ_JAFFHJ010000009.1 (Streptococcus suis)

Compact view

NZ_JAFFHJ010000003.1 - Region 1 - T3PKS,terpene-precursor

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Location: 1 - 34,682 nt. (total: 34,682 nt) Show pHMM detection rules used

Region on contig edge.

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase reductase

JN542_RS04270: hydroxymethylglutaryl-CoA reductase

smcogs: SMCOG1187: hydroxymethylglutaryl-CoA reductase

JN542_RS04305: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: acetyltransferase methyltransferase

JN542_RS04280: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

JN542_RS04375: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

JN542_RS11265: isoprenylcysteine carboxyl methyltransferase

smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

Hydrolases: deacetylase peptidase

JN542_RS04295: polysaccharide deacetylase

smcogs: SMCOG1235: polysaccharide deacetylase

JN542_RS04380: methionine aminopeptidase

smcogs: SMCOG1172: methionine aminopeptidase

Isomerases: mutase

JN542_RS04285: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: synthase synthetase

JN542_RS04225: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

JN542_RS04275: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.42	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.37	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.29	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000205.3	0.29	PKS	bryostatin	Candidatus Endobugula sertula
BGC0002561.3	0.25	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001605.3	0.25	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000286.3	0.23	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001456.3	0.22	terpene	isorenieratene	Streptomyces argillaceus
BGC0001080.5	0.21	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0001595.3	0.21	other (other)	fischerindole	Fischerella sp. SAG 46.79

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.54	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.51	terpene	boleracene	Brassica oleracea var. oleracea
BGC0000866.5	0.48	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0000867.3	0.46	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0002398.2	0.44	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000205.3	0.44	PKS	bryostatin	Candidatus Endobugula sertula
BGC0000278.3	0.43	PKS	urdamycin	Streptomyces fradiae
BGC0001605.3	0.43	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0002561.3	0.42	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001595.3	0.41	other (other)	fischerindole	Fischerella sp. SAG 46.79

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

JN542_RS04225: PT_FPPS_like

Domain 1 (25 - 270): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: DMAPP, IPP → FPP

Transcription Factor Binding Sites

Binding sites found:

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 22.73 out of 29.25

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.45 out of 26.06

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.64 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.30 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.64 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.26 out of 27.78

Confidence: medium

Additional weak hits (3):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.72 out of 26.06

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.22 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.79 out of 29.38

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MogR	Repressor of flagellar motility genes
SypG	Regulator of biofilm formation and host colonization

NZ_JAFFHJ010000004.1 - Region 1 - lanthipeptide-class-i

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Location: 124,732 - 150,743 nt. (total: 26,012 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: protease

JN542_RS06210: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase dehydratase

JN542_RS06250: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

JN542_RS06260: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001789.3	0.75	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000537.3	0.75	ribosomal	nisin U	Streptococcus uberis
BGC0000538.3	0.68	ribosomal	nisin Z	Lactococcus lactis
BGC0000535.4	0.68	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000536.3	0.65	ribosomal	nisin Q	Lactococcus lactis
BGC0000506.4	0.57	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000541.5	0.57	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000542.3	0.57	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000559.4	0.55	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000556.5	0.54	ribosomal	streptin	Streptococcus pyogenes

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001789.3	0.75	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000537.3	0.75	ribosomal	nisin U	Streptococcus uberis
BGC0000538.3	0.68	ribosomal	nisin Z	Lactococcus lactis
BGC0000535.4	0.68	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000536.3	0.65	ribosomal	nisin Q	Lactococcus lactis
BGC0000506.4	0.57	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000541.5	0.57	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000542.3	0.57	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000559.4	0.55	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000556.5	0.54	ribosomal	streptin	Streptococcus pyogenes

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for JN542_RS06250

JN542_RS06245

Leader:

MQNNDFNLDLVSTSKEKKQGASPR

Core:

ITSISLCTAGCITGRIMGCHKTPTVSCNCH

Core with dehydrations:

IDhbDhaIDhaLCysDhbAGCysIDhbGRIMGCysHKDhbPDhbVDhaCysNCysH

antiSMASH-DB 4.0 matches for JN542_RS06245

100.0%

NZ_CP116604.1and 2 others:

NZ_JAFFHI010000008.1 Lanthipeptides: JN541_RS08535_lanthipeptideNZ_JAFFHJ010000004.1 Lanthipeptides: JN542_RS06245_lanthipeptide

Lanthipeptides: PHA78_RS04905_lanthipeptide

ITSISLCTAGCITGRIMGCHKTPTVSCNCH

58.8%

NC_013656.1and 20 others:

NC_020450.1 Lanthipeptides: LILO_RS03015_lanthipeptideNZ_CP009472.1 Lanthipeptides: LG36_RS03420_lanthipeptideNZ_CP010050.1 Lanthipeptides: QI18_RS03300_lanthipeptideNZ_CP015903.1 Lanthipeptides: LLUC08_RS03255_lanthipeptideNZ_CP024954.1 Lanthipeptides: CV702_RS02990_lanthipeptideNZ_CP066300.1 Lanthipeptides: LacL0098_RS03205_lanthipeptideNZ_CP098790.1 Lanthipeptides: M5C73_RS03405_lanthipeptideNZ_JAALEQ010000006.1 Lanthipeptides: HFD15_RS10120_lanthipeptideNZ_JAJONJ010000005.1 Lanthipeptides: LSG16_RS07195_lanthipeptideNZ_JAQDWM010000007.1 Lanthipeptides: PGU01_RS08180_lanthipeptideNZ_JAQDXV010000018.1 Lanthipeptides: PGT80_RS11850_lanthipeptideNZ_JAQDYA010000007.1 Lanthipeptides: PGT72_RS09560_lanthipeptideNZ_JAQDYA010000007.1 Lanthipeptides: PGT72_RS09560_lanthipeptideNZ_JAQEIZ010000019.1 Lanthipeptides: PGX18_RS12025_lanthipeptideNZ_JAQEOU010000022.1 Lanthipeptides: PGV42_RS12330_lanthipeptideNZ_JAQEOV010000015.1 Lanthipeptides: PGW09_RS10765_lanthipeptideNZ_MTJP01000006.1 Lanthipeptides: BW151_RS03795_lanthipeptideNZ_MTJR01000005.1 Lanthipeptides: BW153_RS02980_lanthipeptideNZ_MTJS01000002.1 Lanthipeptides: BW154_RS09850_lanthipeptideNZ_RAGL01000022.1 Lanthipeptides: D6108_RS11395_lanthipeptide

Lanthipeptides: LLKF_RS13210_lanthipeptide

ITSISLCTAGCITGRIMGCH-KTPTVSCNCH

ITSISLCT GC TG +MGC+ KT T +C+ H

ITSISLCTPGCKTGALMGCNMKTATCNCSIH

58.8%

NZ_CP065984.1and 2 others:

NZ_CP095737.1 Lanthipeptides: MXM90_RS03205_lanthipeptideNZ_JAQDXJ010000021.1 Lanthipeptides: PGU19_RS11740_lanthipeptide

Lanthipeptides: I6I21_RS02785_lanthipeptide

ITSISLCTAGCITGRIMGCH-KTPTVSCNCH

ITSISLCT GC TG +MGC+ KT T C+ H

ITSISLCTPGCKTGALMGCNMKTATCHCSIH

with 87 more sequence matches

Only the first 10 are displayed here.

56.7%

NZ_CP024974.1and 1 other:

NZ_JAIMDQ010000006.1 Lanthipeptides: K6V44_RS04045_lanthipeptide

Lanthipeptides: CVO91_RS06555_lanthipeptide

ITSISLCTAGCITGRIMGCHKTPTVSCNCH

+TSISLCT GC TG +M C T +C CH

VTSISLCTPGCKTGILMTC-AIKTATCGCH

55.9%

NZ_CP009913.1and 1 other:

NZ_CP009913.1 Lanthipeptides: SSAL8618_RS00810_lanthipeptide

Lanthipeptides: SSAL8618_RS00815_lanthipeptide

ITSISLCTAGCITGRIMGCHKTPTVSCNCH

ITS SLCT GCITG +MGCH ++ CN H

ITSYSLCTPGCITGVLMGCH-IQSIGCNVH

55.9%

NZ_CP090412.1and 3 others:

NZ_QSTB01000004.1 Lanthipeptides: DXC22_RS03785_lanthipeptideNZ_VSTZ01000009.1 Lanthipeptides: FYK98_RS07445_lanthipeptideNZ_VSTZ01000009.1 Lanthipeptides: FYK98_RS07445_lanthipeptide

Lanthipeptides: LZF92_RS09890_lanthipeptide

ITSISLCTAGCITGRIMGCHKTPTVSCNCH

ITS SLCT GC TG +MGC T SC CH

ITSYSLCTPGCKTGALMGC-TMKTASCGCH

55.9%

NZ_FBTD01000003.1:

Lanthipeptides: PB1E_RS03075_lanthipeptide

ITSISLCTAGCITGRIMGCHKTPTVSCNCH

ITS+SLCT GC TG +MGC+ T SC CH

ITSVSLCTPGCKTGALMGCN-MKTASCGCH

55.9%

NZ_LR793265.1:

Lanthipeptides: HV392_RS09825_lanthipeptide

ITSISLCTAGCITGRIMGCHKTPTVSCNCH

ITS SLCT GCITGR+MGC + + CN H

ITSYSLCTPGCITGRLMGC-QFQSAGCNVH

54.3%

NZ_FNDP01000013.1:

Lanthipeptides: BLP54_RS11230_lanthipeptide

ITSISLCTAGCITGRIMGC-HKTPTVSCNCH

ITS SLCT GC+TG +MGC +KT T +C+ H

ITSKSLCTPGCVTGVLMGCNNKTATCNCSVH

53.3%

NZ_CP086149.1and 13 others:

NZ_CP086149.1 Lanthipeptides: LL127_RS22600_lanthipeptideNZ_CP086149.1 Lanthipeptides: LL127_RS22595_lanthipeptideNZ_CP086149.1 Lanthipeptides: LL127_RS22590_lanthipeptideNZ_CP086149.1 Lanthipeptides: LL127_RS22585_lanthipeptideNZ_CP086149.1 Lanthipeptides: LL127_RS22580_lanthipeptideNZ_CP086149.1 Lanthipeptides: LL127_RS22575_lanthipeptideNZ_CP086149.1 Lanthipeptides: LL127_RS22570_lanthipeptideNZ_CP087100.1 Lanthipeptides: LOR37_RS23225_lanthipeptideNZ_CP087100.1 Lanthipeptides: LOR37_RS23220_lanthipeptideNZ_CP087100.1 Lanthipeptides: LOR37_RS23215_lanthipeptideNZ_CP087100.1 Lanthipeptides: LOR37_RS23210_lanthipeptideNZ_CP087100.1 Lanthipeptides: LOR37_RS23205_lanthipeptideNZ_CP087100.1 Lanthipeptides: LOR37_RS23200_lanthipeptide

Lanthipeptides: LL127_RS22605_lanthipeptide

SLCTAGCITGRIMGCHK

SLCTAGCITGRIMGC+K

SLCTAGCITGRIMGCNK

53.1%

NZ_CP059471.1and 3 others:

NZ_FNZW01000001.1 Lanthipeptides: BMZ61_RS02110_lanthipeptideNZ_FOYC01000001.1 Lanthipeptides: BMZ88_RS03370_lanthipeptideNZ_KE384124.1 Lanthipeptides: G597_RS0106700_lanthipeptide

Lanthipeptides: H1R75_RS06635_lanthipeptide

ITSISLCTAGCITGRIMGCHKTPTVSCNCH

ITS SLCT GC TG +M C T +C CH

ITSKSLCTPGCKTGALMTC-PIKTATCGCH

53.1%

NZ_FNDP01000013.1:

Lanthipeptides: BLP54_RS11235_lanthipeptide

ITSISLCTAGCITGRIMGCHKTPTVS

SISLCT GC TG +MGCHK P+ S

FKSISLCTPGCPTGILMGCHKCPSGS

52.9%

NZ_JADNFO010000025.1:

Lanthipeptides: I2C89_RS09745_lanthipeptide

ITSISLCTAGCITGRIMGCHKTPTVSCNC

ITS SLCT GC TG +M CH T +CNC

ITSYSLCTPGCKTGVLMACH-LKTATCNC

MIBiG 4.0 matches for JN542_RS06245

58.8%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

ITSISLCTAGCITGRIMGCH-KTPTVSCNCH

ITSISLCT GC TG +MGC+ KT T C+ H

ITSISLCTPGCKTGALMGCNMKTATCHCSIH

13.3%

BGC0002111.3 (EVG22_32245)and 1 other:

BGC0002111.3 (multiple genes) Lanthipeptide: andalusicin A or andalusicin B

Lanthipeptide: andalusicin A or andalusicin B

TVSCNC

T+S NC

TISNNC

10.8%

BGC0001229.4 (SBI_06989):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

ITGRIMG

+TG I+G

VTGLILG

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 28.30 out of 36.54

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 20.26 out of 30.22

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.88 out of 29.65

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

JN542_RS06245 - Class I

Cleavage pHMM score: 21.80

RODEO score: 27

Monoisotopic mass: 2962.4 Da

Molecular weight: 2964.5 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 2982.5 Da N = 1; 3000.6 Da N = 2; 3018.6 Da N = 3

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 143 - 229; Score: 67.0

TFBS definitions

Regulator	Description