LOCUS       NZ_CP049945            11329 bp    DNA     linear   CON 20-NOV-2024
DEFINITION  Streptomyces koyangensis strain SCSIO 5802 chromosome, complete
            genome.
ACCESSION   NZ_CP049945
VERSION     NZ_CP049945.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces koyangensis
  ORGANISM  Streptomyces koyangensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces; Streptomyces
            aurantiacus group.
REFERENCE   1  (bases 1 to 6861301)
  AUTHORS   Ding,W.
  TITLE     Genome mining and metabolic profiling illuminate the polycyclic
            tetramate macrolactams from Streptomyces koyangensis SCSIO 5802
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6861301)
  AUTHORS   Ding,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2020) Chinese Academy of Sciences, Key Laboratory
            of Tropical Marine Bio-resources and Ecology, No.164 Xingang west
            Road, Guangzhou, Guangdong 510301, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016803985.1-RS_2024_11_18
            Annotation Date                   :: 11/18/2024 03:33:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,078
            CDSs (total)                      :: 5,988
            Genes (coding)                    :: 5,861
            CDSs (with protein)               :: 5,861
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 60 of 127
            Pseudo Genes (incomplete)         :: 90 of 127
            Pseudo Genes (internal stop)      :: 7 of 127
            Pseudo Genes (multiple problems)  :: 29 of 127
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:34:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5849745
            Orig. end                         :: 5861074
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP049945.1.
            Bacteria and source DNA available from Deep sea sediment.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11329
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="16"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11329
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11329
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6329](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6329)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1031..2503
                     /gene="hemG"
                     /locus_tag="G9U55_RS25720"
                     /old_locus_tag="G9U55_25910"
     CDS             1031..2503
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404755.1"
                     /locus_tag="G9U55_RS25720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G9U55_25910"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_203215997.1"
                     /sec_met_domain="DAO (E-value: 7.1e-10, bitscore: 37.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAETDGNPAGAGGTSPRVVVVGGGVAGLTAALRLLDSGADVTLL
                     EASDRLGGKLRTGEIGGVRVDLGAESMLARRPEAVGLAREAGLADALQPPATASASLWT
                     RDALRPMPKGHVMGVPADPASLAGVLSEEGVARIAEDRTLPRTEVGEDIAVGEYVAARV
                     GREVVDRLVEPLLGGVYAGNAYRISLRAAVPQLFAAAREHTSLTEGVRAIQARTAADPR
                     TDPVFMGIDGGVGRLPLAVADTVRARGGRIRTGTPVTGLARTPGGWRLTTGAPDAPEAL
                     EADAVVLAVPAPRAARLLAAELPAASADLAEIEYASMALMTFAFRADEAGLPQGSGFLV
                     PPVDGRTVKAATFAGRKWRWIAEQEPGLHVVRTSVGRHREEEILGRDDAGLLALARRDL
                     KAATGVDAEPVDAHVTRWDQGLPQYPVGHLARVARIRDQVAKAPGLALCGAAYDGVGIP
                     ACVASATEAARQVMAARPQGDPASGSSGHAGE"
     gene            2508..3224
                     /gene="hemQ"
                     /locus_tag="G9U55_RS25725"
                     /old_locus_tag="G9U55_25915"
     CDS             2508..3224
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951588.1"
                     /locus_tag="G9U55_RS25725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G9U55_25915"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_101280718.1"
                     /transl_table=11
                     /translation="MSAAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRSGYAD
                     EVQDLFDKLAAKDVTVRGTYDVSGLRADADVMIWWHAETADALQEAYNLFRRTRLGRAL
                     EPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYEWYLLPDEERRAMLADH
                     GKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRGSEARRHVREEVPF
                     YTGRRKSVADLVAQLA"
     gene            complement(3238..4878)
                     /locus_tag="G9U55_RS25730"
                     /old_locus_tag="G9U55_25920"
     CDS             complement(3238..4878)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (105-479). E-value:
                     2.2e-19. Score: 62.0. Matches aSDomain:
                     nrpspksdomains_G9U55_RS25730_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008404759.1"
                     /locus_tag="G9U55_RS25730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G9U55_25920"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_203215998.1"
                     /sec_met_domain="PF00561 (E-value: 9.5e-23, bitscore: 79.6,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAPLILTTAGALLLTPLAAPPQAPAAGAPAPPGSAETRGVALAA
                     ERAARNPIAFGPCPDEEGLPRSLECGTVRVPLDYARPEGEQIDLTVSRAKAAGPKGERQ
                     GALVFNPGGPGSSGMLFPLVGIFPEWKRLASVYDLVGYAPRGVGRSAPLSCRDPREKPD
                     GSWQAPEHPTGKQKAERRTKAEAYARGCAEGGGKRLKHVHSLNNARDLDVVRAGLGERK
                     LTFMGASYGSYFGALYAKLFASHVRRMVFDSAVDPSPSRVWYRNNMAQSAAFEKRWADF
                     RRWIASHDAAYGMGTDEAQVLAAYRRAREKLAAEPAGGTVGPAQLQAVMLRAQYYEDYW
                     PSHAGALAAYLKGDPQPLVDMATPLEEGRVEAENARAVYTAVECNDAPWPTRWKTWDRD
                     HTRLARTAPFETWENAWMNLPCAYWKEPRQTPMDVRVAPGELPPTLILAGERDAATPYA
                     GALELRRRLPDAALVTERGAGTHGLAGGPNACVNRHVEAYLIDGKLPARSTDCAARPAP
                     VPESQEAGTRAGGSGPARPRVAGWSPAAR"
     aSDomain        complement(3442..4563)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G9U55_RS25730_Abhydrolase_1.1"
                     /evalue="2.20E-19"
                     /label="G9U55_RS25730_Abhydrolase_1.1"
                     /locus_tag="G9U55_RS25730"
                     /protein_end="479"
                     /protein_start="105"
                     /score="62.0"
                     /tool="antismash"
                     /translation="ALVFNPGGPGSSGMLFPLVGIFPEWKRLASVYDLVGYAPRGVGRS
                     APLSCRDPREKPDGSWQAPEHPTGKQKAERRTKAEAYARGCAEGGGKRLKHVHSLNNAR
                     DLDVVRAGLGERKLTFMGASYGSYFGALYAKLFASHVRRMVFDSAVDPSPSRVWYRNNM
                     AQSAAFEKRWADFRRWIASHDAAYGMGTDEAQVLAAYRRAREKLAAEPAGGTVGPAQLQ
                     AVMLRAQYYEDYWPSHAGALAAYLKGDPQPLVDMATPLEEGRVEAENARAVYTAVECND
                     APWPTRWKTWDRDHTRLARTAPFETWENAWMNLPCAYWKEPRQTPMDVRVAPGELPPTL
                     ILAGERDAATPYAGALELRRRLPDAALVTERGAG"
     misc_feature    complement(4861..4879)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.23"
                     /tool="antismash"
     gene            complement(5001..6329)
                     /locus_tag="G9U55_RS25735"
                     /old_locus_tag="G9U55_25925"
     CDS             complement(5001..6329)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641256.1"
                     /locus_tag="G9U55_RS25735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G9U55_25925"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_203215999.1"
                     /sec_met_domain="DUF692 (E-value: 3.7e-94, bitscore: 313.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPAIAEAVEAMAGIEWVEVVAENVCPGHLPDSLLR
                     LRERGVAVVPHGVGLGLGGAERPDPVRLAALAERAEALGSPLVTEHIAFVRAGGERTAS
                     RGLEAGHLLPVPRTREGLAVLCENVRIAQDQLPVPLAVENIAALFSWPGEELTEGQFLY
                     ELADRTGVRLLIDVANLHTNHVNQGEDPAAALDALPLEALAYVHVAGGFERDGVWYDSH
                     AHPVPEPVLAVLAELATRVRPPGVLLERDERFPSAAELEAEVEKIRRTVTAAGRDRVPV
                     GAGARPGPVPASGAGAATGPVADEAARTRLALGQTALLSALVAGTPAPEGFDRGRLRVQ
                     SRMLTAKRGSVVASLAPELEQILGDTYRPRFLRYAAGRPLTGGYRADALAFALHTLREE
                     PPAGRVVRRELRDWWIERSGRASLVGRPWARWARVARFALLGR"
     gene            6481..7140
                     /locus_tag="G9U55_RS25740"
                     /old_locus_tag="G9U55_25930"
     CDS             6481..7140
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="G9U55_RS25740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="G9U55_25930"
                     /product="hypothetical protein"
                     /protein_id="WP_129847468.1"
                     /transl_table=11
                     /translation="MAEQLDIDHVKVRRFPGFSTLAGRYRARLPNDGRRQYPAGAADAG
                     RSEGVSGVDEAAVESKLGVQAPLGLFNDQVLKYTAVTPGTATEVRLKQTEQFAALEALG
                     KNAVDAVREAHNSALAANENSQGHFYRAAQEQRQALTADLAQADLTWEQREALHDRLER
                     NVGRVDRKDSENKHFQSARPKAVAAVGAAVFMLSLAFVCRAVTGAREGGAEGPDQD"
     gene            complement(7230..7943)
                     /locus_tag="G9U55_RS25745"
                     /old_locus_tag="G9U55_25935"
     CDS             complement(7230..7943)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018894649.1"
                     /locus_tag="G9U55_RS25745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G9U55_25935"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_203216000.1"
                     /transl_table=11
                     /translation="MTSEPLSDSPFRDERTARDEAPQFVLPLVVRLERAEPPALTDALQ
                     TAARAVLTLLSDPRSEGDGVWAEAVRDWQDARIRKVVRRARGGEWRKAAALPGITVEDG
                     GAEVRVYPPVPLDGWPKELAKLQVSGTELEDPEEPGEPAPGVPVLWLNPDVRMSAGKAM
                     AQAGHGAQLAWWQLSGAERNAWREQGFPLAVRTAGAERWKELVESGLPVVRDAGFTEIA
                     PGSCTVVAEHPALVG"
     gene            complement(7974..8843)
                     /locus_tag="G9U55_RS25750"
                     /old_locus_tag="G9U55_25940"
     CDS             complement(7974..8843)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951592.1"
                     /locus_tag="G9U55_RS25750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G9U55_25940"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_129847628.1"
                     /transl_table=11
                     /translation="MVRKAKLRTGTALGALMAVGAVAGCGQSAPDPHDTAPGPRASHSA
                     SPAAPKAPPTLAPGPGGLAPVFANGPRNDGKTVALTFDADMTADQGERAAGGERFDHPE
                     LIASLRHLKVPATVFMTGRWAEEYPDQARAIGADPLFEIANHSYSHHAFTGDCYGLPRV
                     AESEMRADVERAYTAFRTAGVKKAMPYFRFPGGCYDDAALRAVSAAGVTAVQWDVVSGD
                     AFATDADAVAQQVLDGVRPGSVVVMHCTRSAAPTTERALRRIVPALREQGYRFVKVSEL
                     IGAASRKG"
     gene            complement(9023..10387)
                     /locus_tag="G9U55_RS25755"
                     /old_locus_tag="G9U55_25945"
     CDS             complement(9023..10387)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951595.1"
                     /locus_tag="G9U55_RS25755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G9U55_25945"
                     /product="PhoX family protein"
                     /protein_id="WP_129847466.1"
                     /transl_table=11
                     /translation="MSATRRQVLARTGAVAGIAFTGSLSELFAGTAAAKGMGKAGYGPL
                     VPDPKGLLDLPKGFRYRVLSREGDRMLSGEGQVPSNHDGMAAFGGRRGQVHLVRNHENR
                     PTAPIRVPPVRGLTYDPEGMGGCTALALDPAHRVLGERVAIAGTSTNCAGGPTPWGTWL
                     TCEETEDKAGTNGYTKDHGFIFEVDPVDPHRTGAVPLKAMGRFQHEAIAIDPRHGIVYE
                     TEDAFERPFGLFYRFLPEKPRGGLGSLRAGGALQAMRVPGVPDLSAIQETGASFDRVEW
                     VDVPDPLAEETPIRFQDFGPKGITHAQKLEGCYWGGRSVYFVSSFARSSEGSAADHYGQ
                     IWRYDPDRRRLTLVIVFGPDTDVQLPGEEPDNICLAPSGGLMVAEDGEGAQHVYGVTTR
                     GEVYPMARGRQNIGTPEEPEWGEFAGVCFSPDYDTMFVNCYTPGTTFAVTGPWHR"
     gene            complement(10550..10927)
                     /locus_tag="G9U55_RS25760"
                     /old_locus_tag="G9U55_25950"
     CDS             complement(10550..10927)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010641248.1"
                     /locus_tag="G9U55_RS25760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G9U55_25950"
                     /product="hypothetical protein"
                     /protein_id="WP_203216001.1"
                     /transl_table=11
                     /translation="MTTTQATPRTPVPARCSFLRTALALQALTIFVQAVSAGLLLGTEY
                     GEILHSVGARVMYGASMLYLLAAVVAWKPGGGSPRAVGYASGFLVLASVQVVSGIAHLS
                     ALHVPLGVLMFGASLVAVVRR"
ORIGIN
        1 cagggccagt gcgagggcca ggacgaaggg cagcgccgcc ccgaccacca cggtcagcca
       61 gcgcagcgtc gccacgaacg ccgaccagcc gccggagagc gcgtcggtga aggtcgggcc
      121 gtcgtcctcc ttcgactccg tcttcgccgt gcgctcggtg agctggaggg tgaccgtcgc
      181 catcgaggtg cggtcctcca gcgaggactg ctgggccagc agcgcctcca gctccatctg
      241 gcggctgctc agctcgccct ccagtgagac gatgtcgctc agcttctccg cccggtccat
      301 cagctcccgg acccgcgcca cactggcccg ctgcgacttc aggcggctct cgacgtccac
      361 cacctcctcg gtgacgtcct ccgcgctggt ccgccgggag accacctcgc ccccttcacc
      421 gagcgagtcc agcaccgcgt cgtacttctc ctgcgggacg cggaccacca cggtggaccg
      481 ctcgtggttc tgggagtcgc gccgggtcga ctcgttgccg acgtagccgc ccgcgccctc
      541 gaccaccgaa cgggccgagg ccagcgccga gggcacgtcc ctcacccgca ggtggagttc
      601 ggccgtacgg atcaggtgga cgcccttcag ttcggggcgc gccttcccgt ccgccgcggc
      661 ctcgtccccc gactcccgct tcgcgttgcc ggcctgaccg ccgcccgtcc cgtccgcggc
      721 cgacggcgcg gccgcgcggt cctccgccga ggacgccccg gagtccgacc cggccgagca
      781 ccccgcaaga cccagtaccg ccaccgccag caccgccgcc gcctgccgcc ccacgatccg
      841 gccgccgctc gttccccgca tccggctcat ccgccgcccc cttgtgtcct tcaggcgccc
      901 atactgtggc gcctgtgccg ctacgacggg gcagcgcacc ggtgggtttc gcctcccggg
      961 tcgcgaacgg ataacggaac ggcgccgacc cggcgcccgc ccgccccgtc cggtttgaga
     1021 cagtggctcc atgcgcgcgg aaacagacgg aaacccggcg ggagcgggtg gcacgagccc
     1081 ccgtgtggtg gtcgtcggag gcggcgtcgc ggggctcacg gccgcgctgc gcctgctcga
     1141 ctccggggcc gacgtcaccc tcctggaagc ctccgaccgg ctcggcggca agctccgcac
     1201 cggcgagatc ggcggtgtcc gcgtcgacct gggcgccgag tcgatgctcg cccgccgccc
     1261 cgaggcggtc ggcctcgccc gtgaggcggg cctcgccgac gccctccagc cccccgccac
     1321 cgccagtgcc tccctgtgga cccgcgacgc cctgcgcccc atgcccaagg gccacgtcat
     1381 gggcgtcccc gccgaccccg ccagcctcgc cggggtcctc tccgaggagg gcgtcgcgcg
     1441 gatcgccgag gaccgcaccc tgccccgcac cgaggtcggc gaggacatcg ccgtcggcga
     1501 gtacgtcgcc gcccgggtcg gccgcgaggt cgtcgaccgg ctcgtcgaac ccctcctggg
     1561 cggcgtctac gccggcaacg cctaccgcat ctcgctgcgc gccgccgtcc cccagctctt
     1621 cgccgccgcc cgcgagcaca cctccctgac cgagggcgtc cgcgcgatcc aggcccgcac
     1681 cgccgccgac ccgcgcaccg accccgtctt catgggcatc gacggcgggg tcggccgcct
     1741 cccgctcgcc gtggccgaca ccgtccgcgc ccgcgggggc cgcatccgca ccggtacgcc
     1801 cgtcaccggc ctcgcccgca cccccggcgg ctggcgcctg accaccggcg cccccgacgc
     1861 gcccgaggcc ctggaggccg acgccgtcgt cctcgccgtc cccgcgcccc gggcggcccg
     1921 gctgctcgcc gccgaactcc cggccgcctc cgccgacctc gccgagatcg agtacgcctc
     1981 gatggccctg atgaccttcg ccttccgtgc ggacgaggcc gggctgccgc agggcagcgg
     2041 cttcctcgtc ccgccggtcg acggacggac cgtcaaggcc gccaccttcg cgggccgcaa
     2101 gtggcggtgg atcgccgagc aggagccggg gctccacgtc gtccgcacct cggtcggccg
     2161 ccaccgcgag gaggagatcc tcggccgcga cgacgccggc ctcctcgccc tcgcccgacg
     2221 cgacctgaag gccgccaccg gcgtcgacgc cgagcccgtc gacgcccacg tcacccgctg
     2281 ggaccagggc ctgccccagt acccggtcgg ccacctcgcc cgcgtcgccc gtatccgcga
     2341 ccaggtcgcc aaggcccccg gcctcgccct gtgcggtgcc gcctacgacg gcgtcggcat
     2401 ccccgcctgc gtcgcctccg ccaccgaagc ggcgcgccag gtcatggcgg ctcgcccaca
     2461 gggtgacccg gcgtcgggct cgtccgggca cgcgggagaa tgaccccatg agtgctgctc
     2521 ccgagaagat ccccaacgcg ggcaagaagg ccaaggacct caacgaggtc atccgctaca
     2581 ccctgtggtc cgtcttcaag ctccgcgacg tgctccccga ggaccggtcc ggctacgccg
     2641 acgaggtcca ggacctgttc gacaagctcg ccgccaagga cgtcaccgtc cgcggcacct
     2701 acgacgtctc cggcctgcgc gccgacgccg acgtgatgat ctggtggcac gccgagaccg
     2761 ccgacgcgct ccaggaggcg tacaacctct tccgccgcac ccgcctcggg cgcgcgctgg
     2821 agccggtctg gtcgaacatg gcgctgcacc gccccgccga gttcaacaag tcgcacatcc
     2881 cggcgttcct cgccgacgag accccccgcg actacatcag cgtctacccc ttcgtccgct
     2941 cctacgagtg gtacctcctc cccgacgagg agcgccgggc gatgctcgcc gaccacggca
     3001 agatggcgcg cggctacccg gacgtgcgcg ccaacacggt cgcctcgttc tccctcggcg
     3061 actacgagtg gatcctcgcc ttcgaggccg acgagctgta ccgcatcgtc gacctcatgc
     3121 gccacctgcg cggctccgag gcccgccgcc acgtccgcga ggaggtgccc ttctacaccg
     3181 ggcgccgcaa gtccgtggcc gacctggtcg cgcagctcgc ctgagccccg ctccgcctca
     3241 ccgggccgcc ggggaccacc cggcgacccg ggggcgcgcc ggcccgctcc cgccggcccg
     3301 cgtcccggcc tcctgcgact ccggcacggg ggccggccgg gccgcgcagt cggtggagcg
     3361 cgcggggagc ttcccgtcga tcaggtaagc ctccacgtgc cggttgacgc aggcgttcgg
     3421 cccgcccgcc agcccgtgcg tgcccgcgcc ccgctcggtc accagcgcgg cgtccggcag
     3481 ccgtcgccgc agctccagcg cgcccgcgta cggggtcgcc gcgtcccgtt ccccggcgag
     3541 gatcagtgtc ggcggcagct caccgggggc cacccgcacg tccatcggcg tctgccgggg
     3601 ctccttccag tacgcgcacg gcaggttcat ccaggcgttc tcccaggtct cgaacggcgc
     3661 cgtacgggcg agccgggtgt ggtcgcggtc ccaggtcttc catctggtgg gccacggcgc
     3721 gtcgttgcac tccaccgccg tgtacacggc ccgcgcgttc tcggcctcca cccggccctc
     3781 ctccagcggc gtcgccatat cgaccagcgg ctgcggatca cccttcaggt aggccgcgag
     3841 cgcccccgcg tgcgagggcc agtagtcctc gtagtactgg gcccgcaaca tcaccgcctg
     3901 gagctgcgcg gggccgaccg tgcccccggc gggctccgcc gccagcttct cgcgcgcccg
     3961 ccggtacgcc gccagcacct gcgcctcgtc ggttcccatc ccgtacgccg cgtcgtgcga
     4021 ggcgatccac cgccggaagt ccgcccaccg cttctcgaac gccgccgact gcgccatgtt
     4081 gttccggtac cagacacgtg agggcgaggg gtcgaccgcc gagtcgaaga ccatcctccg
     4141 cacgtgcgag gcgaagagct tcgcgtacag cgccccgaag tacgagccgt acgaggcgcc
     4201 catgaacgtc agcttccgct cacccaggcc ggcccgcacc acgtccaggt cgcgggcgtt
     4261 gttcagcgag tggacgtgct tcagccgctt cccgccgccc tcggcgcacc cgcgcgcgta
     4321 cgcctcggcc ttggtcctcc gctccgcctt ctgcttcccg gtcgggtgct cgggtgcctg
     4381 ccagctcccg tccggcttct cgcgcgggtc ccggcaggag agcggcgccg accggccgac
     4441 cccgcgcggc gcgtacccga ccaggtcgta gacggaggcc agccgcttcc actcggggaa
     4501 gatccccacc agcgggaaga gcatccccga ggagccggga ccgcccgggt tgaagaccag
     4561 cgcgccctgc cgctcgccct tcgggcccgc cgccttggcc cggctcaccg tcaggtcgat
     4621 ctgctcgccc tccggccgcg cgtagtccag cgggacccgt accgtgccgc actccaggga
     4681 gcggggcaac ccctcctcgt ccgggcaggg cccgaaggcg atcgggttcc gcgccgcccg
     4741 ctcggccgcc agcgccaccc cgcgcgtctc ggcgctgccg ggcggggccg gagcaccggc
     4801 cgcgggcgcc tgcgggggag ccgcgagcgg ggtgaggagc agcgctccgg cggtggtgag
     4861 gatgagcggc gctcgcatcg cgtccctttc gtccggccga tgacagcctt agggatgatt
     4921 cgcaggccga tcggcgaggg caagcaccgg cgcgcgtgtt ggacggaccc gctcggatcg
     4981 gcccaacacg gccgccggcc tcagcgcccc agcagcgcga agcgcgccac ccgcgcccac
     5041 cgggcccagg gcctgcccac cagggaggcg cggcccgacc gctcgatcca ccagtcccgc
     5101 agctcgcgcc ggaccacccg cccggccggc ggctcctccc gcagggtgtg cagcgcgaag
     5161 gcgagcgcgt ccgcacggta cccgccggtc agcggccggc ccgccgcgta ccgcaggaag
     5221 cgcggccggt acgtgtcgcc gaggatctgc tccagctccg gggcgaggga ggccacgacg
     5281 gagccccgct tggccgtcag catccggctc tgcacccgca gccgcccccg gtcgaacccc
     5341 tccggcgccg gcgtccccgc gaccagcgcc gacaacagcg ccgtctgccc gagggccagc
     5401 cgcgtccgcg ccgcctcgtc cgccaccggg ccggtcgccg cccccgcccc cgacgccggt
     5461 acgggcccgg gccgcgcccc cgcccccacc gggacccggt cccggccggc cgccgtcacc
     5521 gttcgccgga tcttctcgac ctccgcctcc agctcggccg ccgaggggaa ccgctcgtcc
     5581 cgctccagca gcaccccggg cggccgcacc cgcgtggcca gctcggccag gaccgccagc
     5641 accggctccg gcaccgggtg ggcgtggctg tcgtaccaca cgccgtcccg ctcgaacccg
     5701 cccgccacat ggacgtacgc cagcgcctcc agcggcagcg cgtcgagggc ggcggcgggg
     5761 tcctcgccct ggttgacgtg gttggtgtgc aggttggcga cgtcgatcag cagccgcacc
     5821 ccggtccggt cggccagctc gtagaggaac tgcccctccg tcagctcctc gcccggccag
     5881 gagaagagcg ccgcgatgtt ctccaccgcg agcggcacgg gcagctggtc ctgggcgatc
     5941 cgcacgttct cgcagagcac cgcgagtcct tcccgggtcc gggggaccgg cagcaggtgc
     6001 cccgcctcca gcccgcgcga cgccgtccgc tccccgcccg cccggacgaa cgcgatgtgc
     6061 tcggtgacca ggggcgaccc cagcgcctcg gcccgctcgg ccagcgccgc cagccgtacc
     6121 ggatcgggcc gctccgcgcc acccagcccc agccccaccc cgtgcggcac cacggccacc
     6181 ccgcgctccc gcagccgcag cagggagtcg ggcaggtgac cgggacagac gttctccgcc
     6241 accacctcca cccactcgat ccccgccatc gcctcgacgg cctccgcgat cgcgggccgc
     6301 cagccgatcc ccgtccccag ccgttccata ccgccacccc ctccaggaac cccccggtcc
     6361 ctgcgcccac cctgcccgcc accgcccggc ccggaaccgt cacccgcttt cttcagagca
     6421 ggatttgagc ttcgcctgtc tgcggggctg cacgcgaaag gctggcgtgg gtcgcgctga
     6481 atggcagagc agctggacat cgaccatgtg aaggtgcggc gatttccagg attttccacc
     6541 ctggcaggtc gatatcgtgc acgcttgccc aacgatggac ggcgccagta tccggcaggc
     6601 gccgcggatg ccgggcggag cgagggcgtg agcggtgtgg acgaggcagc agtcgaaagc
     6661 aagttggggg tgcaggcccc gcttggcctc ttcaatgacc aggtgctcaa gtacaccgcc
     6721 gtcacgcccg ggacggccac cgaagtacgg ctcaagcaga ccgaacagtt cgccgcgctg
     6781 gaggccttgg gcaagaacgc cgtcgatgcg gtcagggaag cccacaactc tgctctcgcg
     6841 gccaacgaga acagtcaggg ccacttctac cgggccgcgc aggaacagcg gcaggccctg
     6901 accgcagacc tcgctcaggc cgatctcacg tgggagcagc gagaagctct ccacgaccgc
     6961 ctcgagcgga acgtcggacg ggtggaccgg aaggacagcg agaacaagca cttccagagt
     7021 gcgaggccga aggcagtggc agccgtcggt gcggccgttt tcatgctgag cctggccttc
     7081 gtctgccgcg cggtcaccgg cgctcgtgag ggcggcgcag aggggcccga ccaggactga
     7141 ttccgtaatc acgaccgcgt cagggccggt gccaccgggc accggaccga tgttacggcg
     7201 aagagcgatg acgcgaccag ggccgcccct caccccacca gcgccggatg ctccgccacc
     7261 accgtgcacg acccgggcgc gatctccgtg aacccggcgt cccgcaccac cggcagcccg
     7321 ctctccacca gctccttcca gcgctccgcg ccggcggtcc gcaccgccag cgggaacccc
     7381 tgctcgcgcc aggcgttccg ctccgccccc gacagctgcc accaggccag ctgcgcgccg
     7441 tgccctgcct gggccatcgc tttccccgcc gacatgcgta cgtccgggtt gagccagagc
     7501 accgggacgc cgggcgcggg ctcgcccggc tcctcggggt cctccagctc ggtgccggag
     7561 acctggagct tggccagttc cttcggccag ccgtccagcg gcaccggcgg gtagacccgc
     7621 acctcggcgc cgccgtcctc gaccgtgatc cccggcaggg cggcggcctt gcgccactcg
     7681 ccgccgcgag cccgccgcac caccttgcgg atacgcgcgt cctgccagtc ccgcaccgcc
     7741 tccgcccaga cgccgtcccc ctcggagcgg gggtcgctga gcagcgtcag gacggcccgg
     7801 gcggccgtct ggagcgcgtc ggtgagggcg ggcggctcgg cgcgctccag ccggacgacg
     7861 agcggcagca cgaactgcgg tgcctcgtcg cgggcggtgc gttcgtcacg gaagggactg
     7921 tcggagagag gttcgctggt cacggcccca gtctgccaaa cggcgagccc ccctcacccc
     7981 ttccgcgagg cagccccgat cagctcggac accttcacga agcggtatcc ctgctcgcgc
     8041 agggcgggca cgatccggcg cagcgcgcgc tcggtggtgg gggcggcgct gcgggtgcag
     8101 tgcatgacca cgaccgaccc ggggcggacc ccgtccagca cctgctgcgc caccgcgtcg
     8161 gcgtcggtgg cgaaggcgtc cccgctcacc acgtcccact gcaccgccgt caccccggcc
     8221 gccgagaccg cccgcagcgc cgcgtcgtcg taacacccgc cggggaagcg gaagtacggc
     8281 atcgccttct tcaccccggc cgtgcggaac gcggtgtacg cccgctccac gtcggcccgc
     8341 atctcgctct cggccacgcg cggcaggccg tagcagtcgc cggtgaaggc gtggtggctg
     8401 taggagtggt tggcgatctc gaagagcggg tcggcgccga tggcccgcgc ctggtccggg
     8461 tactcctccg cccaccgccc ggtcatgaag acggtggcgg gcaccttcag atggcgcagc
     8521 gaggcgatca gctccgggtg gtcgaaccgc tcaccgccgg ccgctcgctc accctggtcg
     8581 gccgtcatgt cggcgtcgaa ggtgagcgcg acggtttttc cgtcgttccg gggcccgttg
     8641 gcgaagaccg gggcgaggcc gccggggccg ggcgccagcg tgggcggcgc cttcggcgcg
     8701 gcgggggagg cggagtgcga ggcgcggggc cccggtgccg tgtcgtgggg gtccggggcg
     8761 gactggccgc acccggccac cgcacccacc gccatcaggg cacccagcgc cgtaccggtc
     8821 cggagcttcg ccttgcgcac catgctgatc acgccgcgac cctaggatgc gaaaagccga
     8881 atatcgggcg aaacgccacc cggaaggcgc ggcctcaccc gtctcgcccc accgccctcc
     8941 gccgtccggg cgcctggccg ggcccacgag gcggggttgg ggccgggaac ccgcccccga
     9001 ccccgccccc gcccccgtca cgtcaccggt gccagggccc ggtgaccgcg aaggtcgtcc
     9061 ccggcgtgta gcagttgacg aacatcgtgt cgtagtccgg cgagaagcag acgcccgcga
     9121 actcgcccca ctccggctcc tcgggggtgc cgatgttctg gcggccccgc gccatcgggt
     9181 agacctcgcc gcgcgtcgtc acgccgtaca cgtgctgggc gccctcgccg tcctcggcga
     9241 ccatcaggcc gccgctcgga gcgaggcaga tgttgtccgg ctcctcgccc gggagctgca
     9301 cgtcggtgtc ggggccgaag acgatgacca gggtgaggcg gcggcggtcc gggtcgtagc
     9361 gccagatctg cccgtagtgg tcggcggccg agccctcgct gctccgggcg aagctggaga
     9421 cgaagtagac cgagcggccg ccccagtagc agccctccag cttctgcgcg tgggtgatgc
     9481 ccttcgggcc gaagtcctgg aagcggatcg gcgtctcctc ggcgagcggg tcgggcacgt
     9541 ccacccactc cacccggtcg aacgaggcgc ccgtctcctg gatcgccgag aggtcgggca
     9601 ccccgggcac ccgcatcgcc tggagcgcgc ccccggcccg cagcgagccg aggccgccgc
     9661 gcggcttctc gggcaggaag cggtagaaga gcccgaacgg ccgctcgaag gcgtcctcgg
     9721 tctcgtagac gatcccgtgg cgcgggtcga tcgcgatcgc ctcgtgctgg aagcggccca
     9781 tcgccttcag cggcaccgcg cctgtccggt gcgggtcgac cgggtcgacc tcgaagatga
     9841 aaccgtggtc cttggtgtag ccgttggtcc cggccttgtc ctcggtctcc tcacaggtca
     9901 gccaggtgcc ccagggggtc ggcccgccgg cgcagttggt cgaggtcccg gcgatggcga
     9961 cccgctcgcc gaggacgcgg tgcgcgggat cgagggcgag cgccgtacag ccgcccatcc
    10021 cctccgggtc gtaggtcagc ccccgtacgg gcgggacgcg gatgggggcg gtgggccggt
    10081 tctcgtggtt gcggacgagg tggacctggc cccggcgccc gccgaaggcg gccatcccgt
    10141 cgtggttgct cggcacctgg ccctcgccgg agagcatccg gtcgccctcc cgggacagca
    10201 cccggtagcg gaaacctttc ggcaggtcca gcaggccctt cgggtcgggc accagcggac
    10261 cgtacccggc cttccccatc cccttcgcgg cggccgtgcc cgcgaagagt tcggagagac
    10321 tgccggtgaa ggcgataccg gcgacggcac cggtacgggc gaggacttga cggcgtgtcg
    10381 cggacatggg caactccctg ctgcggctct gcggacagga atgtgacccg cacgtgtgta
    10441 ccacgcaccc agccccaccg gaaccaggcg tggccctgga cccggccgtc acagcccacc
    10501 tggcctgcca cgaacggccc tgacaccggg tacggccgac ggcccggcct caccgccgga
    10561 ccaccgccac cagactcgcc ccgaacatca gcacccccag cggcacatgg agcgcggaca
    10621 ggtgcgcgat ccccgacacc acctgcacgg aggccagtac gaggaagccc gacgcgtacc
    10681 cgaccgccct cggcgagccg ccgcccggct tccaggcgac caccgcggcg agcaggtaca
    10741 gcatcgacgc cccgtacatc acccgcgccc cgacgctgtg caggatctcg ccgtactccg
    10801 tgccgagcag cagccccgcc gagaccgcct ggacgaagat cgtcagggcc tggagggcga
    10861 gcgcggtccg caggaacgag cagcgggccg gaacgggcgt gcggggtgtc gcctgggtcg
    10921 tcgtcatgcc gggtccccct cttccgcgcc cgagggcagc ggccgatgtc gaataaggtc
    10981 tcaccagccc gacgacacgg gcctcggaaa ggtaaggcga gggggcgcga gtggggggcg
    11041 ggatcgtcgg cgagcggcgt caactcatca atgtggccta ccgcttgctc ggctcactcg
    11101 ccgaggccga ggacgccgtc caggagacgt acgcccgctg gtacgcgctg cctgatccgc
    11161 gccgggacga gatagccaac cccggcgcct ggctcaccac ggtgaccggc cgagtctgcc
    11221 tcgacgtgct cggctcggca cgcgcccgcc gcgagcgtta cgtcggggcg tggctgcccg
    11281 atccgctgcc cggccgggcc gggcggccgc acggcacgag cggcgaccc
//
