LOCUS       NZ_AP018365            20576 bp    DNA     linear   CON 21-MAY-2024
DEFINITION  Streptomyces sp. SN-593 chromosome.
ACCESSION   NZ_AP018365
VERSION     NZ_AP018365.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. SN-593
  ORGANISM  Streptomyces sp. SN-593
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Panthee,S., Kito,N., Hayashi,T., Shimizu,T., Ishikawa,J.,
            Hamamoto,H., Osada,H. and Takahashi,S.
  TITLE     beta-carboline chemical signals induce reveromycin production
            through a LuxR family regulator in Streptomyces sp. SN-593
  JOURNAL   Sci Rep 10 (1), 10230 (2020)
   PUBMED   32576869
  REMARK    DOI:10.1038/s41598-020-66974-y Publication Status: Online-Only
REFERENCE   2
  AUTHORS   Takahashi,S., Toyoda,A., Sekiyama,Y., Takagi,H., Nogawa,T.,
            Uramoto,M., Suzuki,R., Koshino,H., Kumano,T., Panthee,S., Dairi,T.,
            Ishikawa,J., Ikeda,H., Sakaki,Y. and Osada,H.
  TITLE     Reveromycin A biosynthesis uses RevG and RevJ for stereospecific
            spiroacetal formation
  JOURNAL   Nat Chem Biol 7 (7), 461-468 (2011)
   PUBMED   21642985
  REMARK    Publication Status: Online-Only
REFERENCE   3
  AUTHORS   Panthee,S., Takahashi,S., Takagi,H., Nogawa,T., Oowada,E.,
            Uramoto,M. and Osada,H.
  TITLE     Furaquinocins I and J: novel polyketide isoprenoid hybrid compounds
            from Streptomyces reveromyceticus SN-593
  JOURNAL   J. Antibiot. 64, 509-513 (2011)
REFERENCE   4
  AUTHORS   Takahashi,S., Takagi,H., Toyoda,A., Uramoto,M., Nogawa,T., Ueki,M.,
            Sakaki,Y. and Osada,H.
  TITLE     Biochemical characterization of a novel indole prenyltransferase
            from Streptomyces sp. SN-593
  JOURNAL   J Bacteriol 192 (11), 2839-2851 (2010)
   PUBMED   20348259
REFERENCE   5  (bases 1 to 8833490)
  AUTHORS   Takahashi,S., Ueki,M., Toyoda,A., Ishikawa,J., Sakaki,Y. and
            Osada,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2017) Contact:Shunji Takahashi Chemical Biology
            Research Group, RIKEN Center for Sustainable Resource Science
            (CSRS); Hirosawa 2-1, Wako, Saitama 351-0198, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Phrap v. 0.990329
            Genome Coverage                   :: 16x
            Sequencing Technology             :: ABI 3730
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016756395.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 03:58:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,420
            CDSs (total)                      :: 7,334
            Genes (coding)                    :: 7,181
            CDSs (with protein)               :: 7,181
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 35 of 153
            Pseudo Genes (incomplete)         :: 136 of 153
            Pseudo Genes (internal stop)      :: 8 of 153
            Pseudo Genes (multiple problems)  :: 25 of 153
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:29:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5109215
            Orig. end                         :: 5129791
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP018365.1.
            This work was done in collaboration with Shunji Takahashi, Masashi
            Ueki, Atsushi Toyoda, Yoshiyuki Sakaki and Hiroyuki Osada (RIKEN)
            and Jun Ishikawa (National Institute of Infectious Diseases).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20576
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="linaridin"
                     /region_number="23"
                     /rules="(cypemycin or cypI)"
                     /tool="antismash"
     cand_cluster    1..20576
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cypemycin or cypI)"
                     /kind="single"
                     /product="linaridin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20576
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:10576](+)"
                     /cutoff="20000"
                     /detection_rule="(cypemycin or cypI)"
                     /neighbourhood="10000"
                     /product="linaridin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10576
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cypemycin or cypI)"
                     /neighbourhood="10000"
                     /product="linaridin"
                     /protocluster_number="1"
     gene            1590..3185
                     /locus_tag="RVR_RS21595"
                     /old_locus_tag="RVR_6042"
     CDS             1590..3185
                     /EC_number="1.6.5.9"
                     /GO_function="GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /GO_process="GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130149.1"
                     /locus_tag="RVR_RS21595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_6042"
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="WP_202235421.1"
                     /transl_table=11
                     /translation="MSFPLLTTTAVLPAVGAVATAAVPAARRAAAKYTALAFSLGTLVL
                     AIVAMVRFDPHGDRYQLTESHTWVKSFGLHYDLGVDGIAVVMVALTALLIPFVMLAGWH
                     DADPLEGPDPNRRWRPTQGFFALILLVEAMVVLSFEATDVFLFYIFFEAMLIPMYFLIG
                     GFGDRAHAAGEEEAARQRSYAAVKFLLYNLVGGLIMLAAVIGLYAATASQLGTGTFSLQ
                     EILSARAAGHLHLATSTERWLFLGFFFAFAVKAPLWPLHTWLPNAMGEATSPVAVLITA
                     VVDKVGTFAMLRFCLQLFPEASKWATPVVLVLAVISIIYGALLAVGQRDIKRLIAYASV
                     SHFGFIILGIFAMTSQGQSGATLYMVNHGISTAALMLVAGFLITRRGSRVIADFGGVQK
                     VAPVLAGTFLIGGLATLSLPGLAPFVSEFLVLVGTFSRHPVLGIVATLGIVLAALYVLV
                     LYQRTMTGPVKAGIEGMADLKARELVVVTPLIALLLFLGVYPKPVADIVNPSVGHTLSD
                     VHKTDPKPALEAKK"
     gene            3194..4843
                     /gene="nuoN"
                     /locus_tag="RVR_RS21600"
                     /old_locus_tag="RVR_6044"
     CDS             3194..4843
                     /EC_number="7.1.1.2"
                     /GO_function="GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /GO_process="GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /gene="nuoN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558804.1"
                     /locus_tag="RVR_RS21600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_6044"
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="WP_202238893.1"
                     /transl_table=11
                     /translation="MHTGPAVHTLWTSAASSTDRFPTPHVEYAQLSPVLIVFGVAMLGV
                     LVEAFVPRRSRYTAHVVLAVVGLAAAFAAVVALAEKGYGTTKAHIAAMGAVAVDGPALF
                     LQGTILLVGLVAVFTFAERRLDPKAHGRQVDSFAAQGSAVPGGDQEKAAVRAGFTTTEV
                     FPLLLFAVGGMLVFPAANDLLTLFVALEVFSLPLYILCALARRNRLMSQEAAVKYFLLG
                     AFSSAFLLFGIALLYGYAGTVSYSGISSVIDGTAKITPALADTTGNDALLLIGLAMVAV
                     GLLFKVGAVPFHMWTPDVYQGAPTPVTGFMAAATKVAAFGALLRLLYVVLPGMRWDWRP
                     VMWGVAIATMVIGAVIAVTQTDVKRLLAYSSVAHAGFILAGVIATDRSGVSSVLFYLAA
                     YSFVTLGAFAVVTLVRDAGGEATHLSRWAGLGRRSPLVAAVFAVFLLAFAGIPLTSGFA
                     GKFAVFKAAAQGGAMPLVIVGVISSAIAAFFYIRVIVLMFFSEPKPDGPSVAVPSPLTS
                     TAIAVGVAVTLVLGLAPQYFLDLADKAGVFVR"
     gene            complement(4959..5612)
                     /locus_tag="RVR_RS21605"
                     /old_locus_tag="RVR_6046"
     CDS             complement(4959..5612)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RVR_RS21605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="RVR_6046"
                     /product="hypothetical protein"
                     /protein_id="WP_202235422.1"
                     /transl_table=11
                     /translation="MPENRDEAVEGDGPSTGLDSLCVLDAVICVHFVGANLQRLLVDVL
                     RTARLVLLVPEEVHQEVLGKSRKYGSIEVPWRRLVASECVRVLPALRAAAVSPRLVEVF
                     EEIRGLQFEQALRRPKDLGEAVVIAHAVDLAERGHEVYVAIDDQDGQATSARYDLTVLT
                     IENVLQLAVLQGRFPTDGDLRKAYARLRSYGDGLVDFGLTDLPTTFKEWRAGQF"
     gene            complement(5691..6902)
                     /locus_tag="RVR_RS21610"
                     /old_locus_tag="RVR_6048"
     CDS             complement(5691..6902)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /locus_tag="RVR_RS21610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_6048"
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_202235423.1"
                     /transl_table=11
                     /translation="MGRRVREERERAGYGQRALAELAGFSQPTLHRIELGTRAQVTLAE
                     LDRLADALDVPVRQLTTGSPVRGRVHIAARIDPAVAEDDKERALRLVVEVLELDARLDG
                     GADDAAAAQQHRRRLTAPRPIGTTPEEQGRELATHVRSVLGLGTAPVADVDELIEHLTG
                     VDTAVLRLPQPVDGFTVTDPARDTTLIAVRACDVPERQRFTFAHELGHLLQGDGAEVHQ
                     LGGARTPTEQRCDAFARHLLAPLEGIRTWLGPDGRVGTAAGPSVDERTAALLARHFRVS
                     FPVMLIQLECLDLISTARKDALKGPTGVELSQRYGWGPAYEQEIDVSRTVRPPRRILER
                     AVSAYRGQRIGVRVLAMLEGRGVEETQAALREAGLVPEVRTAKRADVTRLAARSHSRHE
                     SGDR"
     misc_feature    complement(6933..6946)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.32"
                     /tool="antismash"
     misc_feature    7094..7110
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.07"
                     /tool="antismash"
     gene            7348..7527
                     /locus_tag="RVR_RS21615"
                     /old_locus_tag="RVR_6049"
     CDS             7348..7527
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RVR_RS21615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="RVR_6049"
                     /product="hypothetical protein"
                     /protein_id="WP_202235424.1"
                     /transl_table=11
                     /translation="MSPLAAKANDSSELAAVPLRSTDVQRLASTELQPILSVVVVTSTV
                     SVSISATGGEWPSS"
     gene            7708..10020
                     /locus_tag="RVR_RS21620"
                     /old_locus_tag="RVR_6051"
     CDS             7708..10020
                     /NRPS_PKS="Domain: Abhydrolase_1 (464-582). E-value:
                     4.9e-12. Score: 37.9. Matches aSDomain:
                     nrpspksdomains_RVR_RS21620_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /locus_tag="RVR_RS21620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_6051"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_202235425.1"
                     /sec_met_domain="PF00561 (E-value: 5.5e-08, bitscore: 31.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRLQRMLRAAVAPPTVDRGTALTVMERFGAVNHLVASLEYLARP
                     GDRDRGGLNDWQVARGQFARWPRPARAALDAVGRPAVTRTLHVARVAAAGALLLPLPRR
                     GRFAADLVLSASCLALYPRNHYGTDGADQAAFVVQTAATVARAAEGRPRTVDACLWFAG
                     LQSVLSYTASGWVKASSPTWRSGRALPGVLRTASYGEERAWRLVRDHPRAAKAVCACVV
                     ALESAFPAVHLARGRAAKPLVAAAAGFHLANAGVMGLGRFLTGFCALHPAVLYVTGPRE
                     RVLPGGGVQRRDDTFPVAAGAALAAALTAGAVVRARDRRTVLRGRGDERRFVTSAGTTL
                     VHRVTGPPDPGRPRVDPPGPLDPLFDDGTSGARAGVAAAGAVPGGAGQGAHPWPAAGGA
                     GAVDRADGAPTPAGPADPPGTGAGAAPADALPWAGGPVADGVQGAVGGPPGAGGPSIGP
                     AADGGTVVVLCGALADSPDRSEWLVRALARRHRVVTYHRAGQAGSTAAPRTGPHAGPDA
                     GPDAGPGHGLDAAAADLADLVLHVAGGRPAFLVGHGVGGVIAASAATRLEGRLAGLVLV
                     DPHHPGDVPRPDPDEGPGGTAADDPAGAPDQREVTALLDQMTVSLRLGLGQLLRDPDWL
                     RLLPPDVRGLARAHYRDAAGWSAARGEWRALRARAAAARPGEDAVPAAAVPTCLVLCEP
                     GGAPPDARRALQEQYARRAPHAVVHAVDASRDDVLAARTSARAVAELVTAFAAGSGGPG
                     RDGTDQREEAADVPAQR"
     aSDomain        9100..9453
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_RVR_RS21620_Abhydrolase_1.1"
                     /evalue="4.90E-12"
                     /label="RVR_RS21620_Abhydrolase_1.1"
                     /locus_tag="RVR_RS21620"
                     /protein_end="582"
                     /protein_start="464"
                     /score="37.9"
                     /tool="antismash"
                     /translation="VVVLCGALADSPDRSEWLVRALARRHRVVTYHRAGQAGSTAAPRT
                     GPHAGPDAGPDAGPGHGLDAAAADLADLVLHVAGGRPAFLVGHGVGGVIAASAATRLEG
                     RLAGLVLVDPHHPG"
     CDS_motif       9343..9405
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_RVR_RS21620_0001"
                     /evalue="2.50E-03"
                     /label="NRPS-te1"
                     /locus_tag="RVR_RS21620"
                     /protein_end="566"
                     /protein_start="545"
                     /score="10.5"
                     /tool="antismash"
                     /translation="PAFLVGHGVGGVIAASAATRL"
     gene            10001..10576
                     /locus_tag="RVR_RS21625"
                     /old_locus_tag="RVR_6053"
     CDS             10001..10576
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     linaridin: cypI"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RVR_RS21625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="RVR_6053"
                     /product="hypothetical protein"
                     /protein_id="WP_202235426.1"
                     /sec_met_domain="cypI (E-value: 1.7e-28, bitscore: 98.4,
                     seeds: 22, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPLSGEGPAAGRDLVGAAAIGALAAWFAATVLSQHPRPEFNRIA
                     VLDGTGALIPNWRFFAPEPARHDFHVLFRTEDARGRATPWQEGSAITPRRPLHLVWFPN
                     RRREKALYDLCHELVAVLGQFDGDVTRTPAYRMLRNSVARAVSGLPGRPPRGFQFVVAR
                     HTGHDPGHDPDYLFVSPYIPYTADAPYA"
     gene            complement(10600..11409)
                     /locus_tag="RVR_RS21630"
                     /old_locus_tag="RVR_6055"
     CDS             complement(10600..11409)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020137925.1"
                     /locus_tag="RVR_RS21630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_6055"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_202235427.1"
                     /transl_table=11
                     /translation="MGDFDAFEREAWAGRAEAYARSFGLLCAHTVPMLLDAAGVGAGVR
                     VLDVGTGPGTAAALAVRRGAEVHAVDAEPSMVERAARAVPEAAVQHAMLPELPFADGEF
                     DAVVANFVVNHVGRPADALAELRRVAGPGGRVAVTIWSAPPAPGQALLGRALEAAGAAR
                     PSSVPTLAAEDDFARTEEGLTGLLTAAGLVGARCATLSWNHVADPEVWWSGPAAGIATV
                     GQILHSLPPDTAAEVRRQYDVLAAEFRTDSGALSLPHTALLGWATTS"
     gene            complement(11752..12696)
                     /gene="egtD"
                     /locus_tag="RVR_RS37805"
     CDS             complement(11752..12696)
                     /EC_number="2.1.1.44"
                     /GO_function="GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="egtD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994591.1"
                     /locus_tag="RVR_RS37805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="WP_237405362.1"
                     /transl_table=11
                     /translation="MPAGYFTDTLHADVVGGLTAAPRTLPPKWFYDKRGSDLFEQITRL
                     PEYYPTRAEQEILVRRAPEIAAATRAATLIELGSGSSRKTRLLLDALTAGGTLRRYAPL
                     DVSASALEAAGRAICSDYPGLEVSATVADFEGGIPLSDEPGPRLLAFLGSTIGNFDGPQ
                     RAAFYRTLTRSLSSDDVLLLGADLVKDPAVLVRAYDDAAGVTAAFDKNVLHVLNRELGA
                     DFDPDAFEHVAVWNAEDERIEMHLRSRVAQTVKIPDLDLSVDLAAGEDLRTELSCKFRR
                     ESLTAELHAGGFTVRHWWTDAAARFALLLAVPN"
     gene            complement(<12853..13662)
                     /locus_tag="RVR_RS37810"
                     /pseudo=""
     CDS             complement(<12853..13662)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /locus_tag="RVR_RS37810"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="class II glutamine amidotransferase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MCRHLAYVGPPLPLARLLVEPPRGLYEQSWQPRRQRHGTVNADGF
                     GVGWYPADSEAAVPGEPGAAAVPGPPAPHGPAPHDPAPHDPAPPVPAAGGPAAPAERPG
                     DEGAAVPARYRRALPVWADPNFAELARVIRSGAVLAAVRSATEGTAPDESAAAPFRDGR
                     WLFSHNGAVPDWTRLPTGLGSAELLSLEARSDSALLWAMLAGRLRRGEPPGGAIAAVVR
                     EVAAARPTARLNFLLTDGRTIAASAWGDTLWYRARPGAVVVASEPDDA"
     gene            complement(13662..15038)
                     /gene="egtB"
                     /locus_tag="RVR_RS21640"
                     /old_locus_tag="RVR_6057"
     CDS             complement(13662..15038)
                     /GO_process="GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="egtB"
                     /gene_functions="other (smcogs) SMCOG1226: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848211.1"
                     /locus_tag="RVR_RS21640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_6057"
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="WP_237405363.1"
                     /transl_table=11
                     /translation="MAALRAARHRTGLLTDSVTDDDLVAQHSRLMSPLVWDLAHIGNQE
                     ELWLVREAGRRDGVRPDLDHLYDAFRHPRADRPALPLLGPAQARAYVAEVREETLDVLS
                     GIDTDPAHPERLLKGGFVFGMLAQHEAQHGETMLATHQLRSGPAVLDAPAPPPAPADAA
                     GLPREVLVPGGPFTMGTSTEPWALDNELPAHEVRVAPFWLDTVPVTNGRYADFIDAGGY
                     RDPRWWTPEGWRHVQDAALTAPLFWSRDGDRWIRRRFGHVEAVPADEPVVHVCWYEADA
                     YARWAGRRLPTEAEWEKAARFDPASGRSRRYPWGDADPGPEHANLGQRHLRPAAAGSYP
                     EGAAPSGARQLIGDVWEWTSSDFAPYPGFAAFPYKEYSEVFFGPEYKVLRGGAFGVDPV
                     ACRGTFRNWDYPIRRQIFAGFRTARDLTDTEARTAPGTAAIANGASRAGGSGATGGGIV
                     "
     misc_feature    complement(15149..15163)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     misc_feature    complement(15176..15192)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.93"
                     /tool="antismash"
     gene            complement(15243..16676)
                     /gene="egtA"
                     /locus_tag="RVR_RS21645"
                     /old_locus_tag="RVR_6059"
     CDS             complement(15243..16676)
                     /EC_number="6.3.2.2"
                     /GO_function="GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="egtA"
                     /inference="COORDINATES: protein motif:HMM:TIGR03444.1"
                     /locus_tag="RVR_RS21645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_6059"
                     /product="ergothioneine biosynthesis glutamate--cysteine
                     ligase EgtA"
                     /protein_id="WP_202235430.1"
                     /transl_table=11
                     /translation="MAVNRADRREDLTEDEAEAHVHGVCFKTGPPERTGVELEWLVQDR
                     SDPRALIPVERLDAALAPVEAPGALPRGSRLTREPGGQVELSSPPAPSLAGCVAAMAED
                     LAALREAVERAGLVLVGHGLDPHREPPRVLDHPRYRAMERFFDREGPWGRTMMRRTASL
                     QVNLDSGEDRPGVTGYRGRWALAHRIGPVMVAAFANSPFRDGRPTGWVSTRQANWALMD
                     PGRTRQPRHDGPDEDPRAAWARYALDARVLCIRQDGGTVGPAAEHATRPGASSAGGRTA
                     ASAAPGRGSSATGSLKGSLVGSPSGPDRTDSGDWSAPHGLTFRQWVRGAPGVRRPTVAD
                     LDYHLSTLFPPVRPRGWLELRMTDAQDGDQWIVPTVLAATLLDDPVAAETAYAATEPLA
                     TSGDPLPTDLVWRRAARTGLADPELARAARACFAAAESALARTGAPPALRQAVSDFAER
                     YPQRGRCPAHDQLDALSRA"
     misc_feature    16864..16877
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.71"
                     /tool="antismash"
     misc_feature    complement(16882..16895)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.47"
                     /tool="antismash"
     misc_feature    complement(16941..16956)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.79"
                     /tool="antismash"
     misc_feature    complement(16941..16956)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.05"
                     /tool="antismash"
     gene            17093..18112
                     /locus_tag="RVR_RS21650"
                     /old_locus_tag="RVR_6060"
     CDS             17093..18112
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PT_FPPS_like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PT_HPP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948714.1"
                     /locus_tag="RVR_RS21650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_6060"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_202235431.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 8.5e-57, bitscore:
                     191.1, seeds: 145, tool: rule-based-clusters)"
                     /sec_met_domain="PT_HPP (E-value: 1.3e-48, bitscore: 164.5,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVVGPFGLSVWDENLDADVQAGLAAVEEGLLEATKSDVPFITDT
                     ARHLLRAGGKRFRPLLVMLGAQFGDPHAPGVVPAAVVVELTHLATLYHDDVMDEADVRR
                     GVPSANSRWDNSVAVLTGDFLFSRASHILADLGPEAVRVQAEAFERLVTGQIMETVGPR
                     DGRDPLDHYLEVIAGKTSSLMAVSGRFGAMMSGADESVVHVLTQYGERIGVAFQLADDV
                     LDIASDSHESGKTPGTDLREGIPTLPVLHVRRWRAQGKGPATPEDRRLNELLDGDLTDD
                     ALHAEALHLLRAHPALEQARRDTVRYADEARAALAPLPDLPAKASLAALCEAVVHRAG"
     misc_feature    18522..18541
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: medium, score: 24.85"
                     /tool="antismash"
     gene            18591..19853
                     /locus_tag="RVR_RS21655"
                     /old_locus_tag="RVR_6061"
     CDS             18591..19853
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018101323.1"
                     /locus_tag="RVR_RS21655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_6061"
                     /product="DUF2092 domain-containing protein"
                     /protein_id="WP_237404889.1"
                     /transl_table=11
                     /translation="MAVTERIGRPEPEEPERPRGRARKIARYAVPVAVAGVAAATITLV
                     PALADAGDPSLPKITAQQLLTKIAASDTRALDGTVKVSTDLGLPDALSGGAAGGLLSGA
                     GGSGKDGSSAAPQAQLAQLLVGTHQLHVAVDGQDRQKVSVIEPAAEYSVIHDGTQLWAY
                     DSASNQAYHATLPAAADESSGPVGARPSDGVPTTPQAAAEQVLRAARGTSSVTVDGTAR
                     VAGRSAYELVVTPEHAADTTVGSVRIAVDSGTGVPLKFTVLPKGGGKAVFEIGFTKVGF
                     GEPAASTFSFTAPKGAEVTEGKSSGASGGSGAGAVAGLAAPTVLGTGWDSVAVIDTGDA
                     GSGSGDGGGSGFGADGTAGSGRGGDASSALDGYGTKVTGSFGTGTVFHTRLVNVLLTDE
                     GRLYVGAVTESALTAAAGGHH"
ORIGIN
        1 acatggagca cgacgagcgc cgccgccgct tcttcggcta cctcaacctc ttcctcgcgg
       61 cgatgctgct gctggtgctc gccgacaact acctgctgct gtacgcgggg tgggagggcg
      121 tcgggctggc gtcctacctg ctgatcggct tctggcagca caagccgagc gcggcgaccg
      181 ccgcgaagaa ggcgttcctg gtcaaccggg tcggcgacat ggggctgtcc atcggcatca
      241 tgctgatgtt caccaccttc gggtcgttcg ccttcgccga cgtgctgccg gccgccgcgg
      301 gtgcctcggc cacccagcac gccaccgtca ccggcatcgg cttcatgctg ctgctcgccg
      361 cgtgcggcaa gtcggctcag gtgccgctcc agtcctggct cggcgacgcg atggagggcc
      421 cgaccccagt ctcggccctg atccacgccg cgaccatggt gaccgcgggc gtctacctga
      481 tcgtccgctc gggcgcgatc ttcgacgcgg cgccggacgc gcagaccgcg gtggtcaccg
      541 tcggcgcggt cacgctgctc ttcggtgcga tcgtcggttg cgccaaggac gacatcaaga
      601 aggcgctcgc cggctccacg atgtcgcaga tcggctacat gatcatggcc gccgggctcg
      661 gcccgatcgg ctacgtcttc gcgatcatgc acctggtcac gcacggcttc ttcaaggccg
      721 ggctgttcct cggggccggc tcggtgatgc acgggatgaa cgacgaggtg gacatgcgcc
      781 gctacggcgg cctgcgcacg tacatgccga tcaccttcgt caccttcggc ctgggctacc
      841 tggcgatcat cggcttcccg gggctgtccg gcttcttctc caaggacaag atcatcgagg
      901 cggcgtacgc caagggcggc accgagggct ggatcctcgg cagcgcggcc ctgctgggcg
      961 ccgcggtcac cgcgttctac atgacgcggg tgatgctgat gaccttcttc ggcgagaagc
     1021 gctgggtgcc ggacgcggag ggccacgagc cgcacccgca cgagtcgccg aagtcgatga
     1081 ccgtcccgat gatgatcctc gccctcggct cggtcttcgc cggcgccgcc ttcagcatca
     1141 actcctcgtt cctgaagtgg ctggagccgg tcaccgggca cagcgagggc gactcgccgg
     1201 tgagcgccgg caccgtcacc gccgccacca tggtctgcct ggtggtcggc gtcggcatcg
     1261 cctggctgat gtacgggcgc aggccggtgc cggcggtcgc gccgcgcggc agcctggcca
     1321 cccgggcggc ccgccgcgac ctgctccagg acgacttcaa ccacgtggtg ctggtgcggg
     1381 gcggcgagca cctgacccgc tcgctggtct acctcgacca ctccctggtc gacggcgtcg
     1441 tcaacggcac ggccgcctcc gtcggcggcc tgtcggggcg gctgcgccgg ctccagaacg
     1501 gctacgtcag gtcgtacgcc gtctccatgc tcggcggtac cgcggtggtg gtcgccgcga
     1561 cccttctcat gagggcggtc tgatagctca tgtcgtttcc tctgctgacc accaccgcgg
     1621 tactgcccgc ggtcggcgcc gtcgccaccg ccgcggtgcc cgccgcgcgc agggccgccg
     1681 ccaagtacac ggcactggcc ttctcgctgg gcacgctggt gctggcgatc gtcgcgatgg
     1741 tgcggttcga tccgcacggc gaccgctacc agctcaccga gtcgcacacc tgggtgaaga
     1801 gcttcggcct ccactacgac ctgggagtcg acggcatcgc ggtcgtcatg gtcgcgctga
     1861 ccgcgttgct gatcccgttc gtgatgctcg ccggctggca cgacgccgac ccgctggagg
     1921 ggcccgaccc caaccggcgc tggcggccca cccagggctt cttcgcgctg atcctgctgg
     1981 tcgaggcgat ggtggtgctc tccttcgagg ccaccgacgt cttcctcttc tacatcttct
     2041 tcgaagccat gctgatcccg atgtacttcc tcatcggcgg cttcggcgac cgggcgcacg
     2101 cggccggtga ggaggaggcg gcccgccagc ggtcgtacgc ggcggtgaag ttcctgctct
     2161 acaacctggt cggcgggctg atcatgctgg ccgcggtgat cgggctgtac gccgccaccg
     2221 ccagccaact gggcaccgga accttctcgt tgcaggagat cctgtccgcc cgcgccgccg
     2281 gtcacctgca cctggccacc tccaccgagc ggtggctgtt cctcggcttc ttcttcgcct
     2341 tcgcggtgaa ggcgccgctg tggccgctgc acacctggct gccgaacgcg atgggcgagg
     2401 ccacctcgcc ggtggcggtg ctgatcaccg cggtggtgga caaggtcggc accttcgcga
     2461 tgctgcgctt ctgcctccag ctcttcccgg aggcgtcgaa gtgggcgacc ccggtggtcc
     2521 tggtgctcgc ggtgatcagc atcatctacg gcgccctgct cgcggtcggc cagcgcgaca
     2581 tcaagcggct gatcgcgtac gcgtcggtgt cgcacttcgg cttcatcatc ctgggcatct
     2641 tcgcgatgac cagccagggc cagtccggcg cgacgctcta catggtcaac cacggcatct
     2701 ccacggccgc gttgatgctg gtcgccgggt tcctgatcac ccggcgcggg tcgcgggtca
     2761 tcgccgactt cggcggggtg cagaaggtcg cgccggtgct cgccggcacc ttcctgatcg
     2821 gcggcctggc cacgctgtcg ctgccggggc tcgcgccgtt cgtcagcgag ttcctggtgc
     2881 tggtcggcac gttcagccgc catccggtgc tgggcatcgt cgcgaccctc ggcatcgtgc
     2941 tcgccgcgct gtacgtgctg gtgctctacc agcgcaccat gaccggcccg gtcaaggccg
     3001 gcatcgaggg catggccgac ctgaaggcac gcgaactggt ggtggtcacg ccgctgatcg
     3061 cgctgctgct cttcctcggc gtgtacccga agccggtggc ggacatcgtc aacccgtcgg
     3121 tcgggcacac gctctccgac gtgcacaaga ccgaccccaa gcccgcgctg gaggcgaaga
     3181 agtgagcgcc gcactgcata cgggacccgc cgtccacacc ctgtggacga gcgccgcgtc
     3241 ctccacggac cgcttcccca cgccgcacgt cgagtacgcg cagctctcgc cggtgctgat
     3301 cgtcttcggc gtggcgatgc tcggggtgct cgtcgaggcg ttcgtgcccc gccggtcccg
     3361 ctacaccgcg cacgtggtgc tggccgtggt cggtctggcc gccgcgttcg ccgcggtggt
     3421 ggcactcgcc gagaaggggt acggcaccac caaggcgcac atcgcggcga tgggcgcggt
     3481 cgcggtggac gggccggcgc tgttcctcca gggcaccatc ctgctggtgg ggctggtcgc
     3541 ggtgttcacc ttcgccgagc gccgcctcga ccccaaggcg cacggccggc aggtcgactc
     3601 cttcgccgcc cagggctccg cggtgcccgg cggcgaccag gagaaggccg cggtgcgcgc
     3661 cgggttcacc accaccgagg tcttcccgct gctgctcttc gcggtcggcg ggatgctggt
     3721 cttcccggcg gcgaacgacc tgctgacgct cttcgtcgcg ctggaggtct tctcgctgcc
     3781 gctgtacatc ctgtgcgcgc tggcccgccg caaccggctg atgtcgcagg aggccgcggt
     3841 caagtacttc ctgctcggcg ccttctcgtc ggcgttcctg ctcttcggta tcgcgctgct
     3901 gtacgggtac gcgggcaccg tgtcgtactc cgggatctcc tcggtgatcg acggcaccgc
     3961 gaagatcacc ccggcgctgg ccgacaccac cgggaacgac gcgctgctgc tgatcgggct
     4021 ggcgatggtc gcggtcgggc tgctgttcaa ggtcggcgcg gtgcccttcc acatgtggac
     4081 gccggacgtc taccagggcg cgcccacccc ggtcaccggg ttcatggccg cggccacgaa
     4141 ggtcgccgcg ttcggcgcgc tgctgcgact gctgtacgtg gtgctgcccg gcatgcgctg
     4201 ggactggcgg ccggtgatgt ggggcgtggc gatcgccacg atggtcatcg gcgcggtcat
     4261 cgcggtcacc cagaccgacg tgaagcggct gctcgcgtac tcgtcggtcg cgcacgccgg
     4321 gttcatcctg gccggcgtga tcgccaccga ccggtcgggc gtctcgtcgg tgctcttcta
     4381 cctcgccgcg tactccttcg tgaccctcgg cgcgttcgcg gtggtcaccc tggtgcgcga
     4441 cgcgggcggc gaggccaccc acctgtcccg ctgggcgggt ctgggccgcc gctcgcccct
     4501 ggtcgccgcg gtcttcgcgg tgttcctgct ggcgttcgcc ggcatcccgc tgacgtccgg
     4561 gttcgcgggg aagttcgcgg tcttcaaggc ggcggcgcag ggcggcgcga tgccgctggt
     4621 gatcgtcggg gtgatctcgt cggcgatcgc ggcgttcttc tacatccgcg tcatcgtgct
     4681 gatgttcttc agcgagccca agcccgacgg cccctcggtc gcggtgccct cgccgctgac
     4741 ctcgaccgcg atcgcggtgg gcgtggcggt cacgctggtg ctgggcctgg ccccgcagta
     4801 cttcctggac ctggccgaca aggcgggggt gttcgtccgc tagggccgca cccgccgcac
     4861 ccgccgcaca ccgaccaccg aaggggcccg cccaccaccc cgggcccctt cccccgtccc
     4921 gcccccaccc ccgggaagcg ggactttgcg ggtgcaccct agaactggcc ggcccgccat
     4981 tccttgaacg tcgtgggcag gtcggtgagg ccgaagtcca ccagcccgtc accgtaggac
     5041 cgcaacctcg cgtacgcctt ccggagatcg ccgtccgtgg ggaacctgcc ctgcaggacc
     5101 gccagttgca ggacgttctc gatggtcagc acggtcaggt cgtaacgtgc cgaggtggct
     5161 tggccgtcct ggtcatcgat ggcgacgtac acctcatgtc cgcgttcggc aagatccacg
     5221 gcgtgggcga tgaccaccgc ctcgcccagg tccttgggac ggcgcaacgc ctgttcgaac
     5281 tgcaacccgc ggatctcctc gaacacctcg acgagacgcg gggacacggc tgccgcgcgc
     5341 agtgcgggaa gcacacgcac gcactcgctc gcgaccaacc gacgccaagg aacctcgatc
     5401 gaaccgtact tgcgcgactt gccgagcacc tcctgatgca cctcctccgg cacgagcagt
     5461 accaggcggg cggtccgcag cacgtcgacc agaagccgct gcagattcgc ccccacgaag
     5521 tggacgcaga tgaccgcatc gagcacgcac agcgagtcga gcccggtgga cggaccatcg
     5581 ccctcgacgg cctcgtcacg gttctcgggc aatccccacc ccttcgttcg gcagccctgt
     5641 acagcctcga cggcacgtca ccaagggatg gcggttgccg tctgcgagtc ctagcgatcc
     5701 ccggattcgt gtcgggagtg tgatctggcg gcgaggcggg tcacgtcggc ccgcttggcc
     5761 gtacgcactt cgggcacgag gccggcctcc cgcagggcgg cctgggtctc ctcgacaccg
     5821 cgcccctcca gcatcgccag cacgcggacg ccgatccgtt gcccgcggta cgcgctgacc
     5881 gcccgttcca ggatgcgtcg aggaggccgc accgtccggg agacgtcgat ttcctgctcg
     5941 tacgcgggcc cccagccgta gcgctgcgag agttcgacgc cggtgggccc cttgagagcg
     6001 tccttccgag cggtggagat gaggtccagg cactccaact ggatgagcat caccgggaaa
     6061 ctcacccgga agtgccgggc gaggagggcc gcggtccgct cgtccacgct cggaccggcc
     6121 gcggtgccga cccgtccgtc aggccccaac caggtgcgga tgccttccag gggggccagc
     6181 agatgccgtg cgaacgcgtc gcagcgctgc tcggtcggtg tcctggcccc acccagttgg
     6241 tgcacctcgg cgccgtcgcc ctggagaagg tgtccgagtt cgtgggcgaa cgtgaacctc
     6301 tggcgctcgg gtacgtcgca cgcgcgcacc gcgatgagcg tcgtgtcccg cgcggggtcg
     6361 gtcacggtga agccgtcgac gggttgcggc aggcgtagca ccgccgtgtc cactccggtg
     6421 aggtgctcga tcagctcgtc cacatcggcc accggcgcgg tgccgaggcc gagcacggag
     6481 cgcacgtgtg tcgcgagttc ccgaccctgc tcctcgggag tggtgccgat cgggcgcggt
     6541 gcggtgagac gcctgcggtg ctgctgagcg gccgctgcgt catcggcgcc gccgtcgagg
     6601 cgcgcgtcga gttccagtac ctccacgacc agtcgcagcg cgcgctcctt gtcgtcctcg
     6661 gcgacggccg ggtcgatgcg ggcggcgatg tgcacgcgcc cccgcacggg gctccccgtg
     6721 gtgagctgcc gtaccggtac gtccagcgcg tccgccagtc ggtcgagctc ggcaagggtc
     6781 acctgcgccc gcgtaccgag ttcgatccgg tggagagtcg gctgggagaa gccggcgagc
     6841 tcggcgaggg ctcgctggcc gtagcccgcg cgctcgcgct cctcgcggac tcgccgtccc
     6901 acagctacaa gatccacaga gcacctcctt gcttgaatca attctacgcg ggtgattcac
     6961 gctgtccacc ggtatgtcta ccaggggggt ctgacagcgg ggcggcggat cgtcgcgggg
     7021 cggctgtacc gggcggcgtg gtccccttgg cgacctccat ggaaagcggg tgtcaactca
     7081 ggtgaatatc gggttgtttc tgattcacat cagttgacct ttgctcgatg ggtgggcaat
     7141 gctgaacgcc gtcgccggcg gcagcggtcc tgccgtacgg cggcggaacg cggatacgcg
     7201 tcccgggtac ggtccgggcg cggtgcaccc gcaccggcgc tcctcggccg ggtccgagcc
     7261 gggcccgacc ggcgcggctc cgggccggaa ccgggcccga ccgcaacggc ggccggccgc
     7321 cggccggcca cgaccgaagg agagcccatg tcccctcttg ccgcgaaggc gaacgacagc
     7381 agcgaactgg cggccgtgcc gctgcggtcc accgacgtgc agcggctggc gagcaccgag
     7441 ctccagccca tcctgtccgt cgtcgtggtg acctccaccg tcagtgtctc gatcagcgcc
     7501 accggcggcg agtggccgtc gtcgtaagga ccgttgcgcg gtcccggtcc ggttccgttc
     7561 ccggttccgg ccggtgacgc ggcgtcggcg ccctccccgt ccggtggcgg cgagggcgcg
     7621 cggcgctcga cgcgttaccg cgctccgcgt catccgcacc cccgcgtcga cccgcgcccc
     7681 gcgcccaccc agagaagggg aagacccgtg ccgagactcc agcggatgct gcgtgccgcg
     7741 gtggcaccgc ccacggtgga ccgcgggacc gcgctgaccg tcatggagcg gttcggcgcg
     7801 gtcaaccacc tggtggcgag cctggagtac ctggcccggc ccggcgaccg cgaccggggc
     7861 ggcctcaacg actggcaggt cgcccgcggg cagttcgcgc gctggccgcg cccggcccgt
     7921 gccgcgctcg acgccgtggg ccggcccgcc gtgacccgca cgctgcacgt cgcccgcgtc
     7981 gccgcggccg gcgccctgct cctgcccctg ccccgccgcg gccggttcgc cgccgacctg
     8041 gtgctcagcg cctcctgcct cgcgctgtac ccgcgcaacc actacggcac cgacggcgcc
     8101 gaccaggccg cgttcgtcgt gcagaccgcg gcgacggtgg cgcgggcggc cgagggccgg
     8161 ccccgtaccg tggacgcctg cctgtggttc gccggcctcc agtcggtgct gtcctacacc
     8221 gcctccggct gggtgaaggc gtccagcccg acctggcgca gcggccgtgc cctgcccggc
     8281 gtgctgcgca ccgcctccta cggcgaggag cgcgcctggc ggctggtccg cgaccatccg
     8341 cgcgccgcga aggcggtgtg cgcctgcgtg gtggcgctgg agtccgcgtt ccccgccgtc
     8401 cacctggccc gcggccgcgc cgccaagccg ctggtcgccg ccgccgcggg cttccacctg
     8461 gccaacgccg gcgtgatggg cctgggccgc ttcctcaccg gcttctgcgc gctgcacccg
     8521 gcggtgctct acgtcaccgg tccgcgtgaa cgcgtcctgc ccggcggcgg ggtgcagcgg
     8581 cgcgacgaca ccttcccggt cgccgccggc gccgcgctgg ccgcggccct gacggccggg
     8641 gccgtcgtgc gcgccaggga ccggcggacg gtgctgcgcg ggcgcggcga cgaacggcgg
     8701 ttcgtcacct cggccggcac caccctcgtc caccgcgtga ccggtccgcc cgaccccggt
     8761 cggccccggg tggacccgcc cggcccgctc gacccgctct tcgacgacgg aacgtcgggc
     8821 gcccgcgcgg gcgtggccgc ggccggcgcc gtccccgggg gagccgggca gggcgcgcac
     8881 ccctggcccg ccgcgggagg ggcgggtgcc gtggaccgcg ccgacggggc gccgacgcca
     8941 gcgggcccgg ccgacccgcc cgggaccggc gccggcgccg cccccgcgga cgccctgccg
     9001 tgggcgggcg gcccggtggc cgacggtgtc cagggcgccg tgggcggccc tcccggcgcg
     9061 ggcggcccgt ccatcggccc tgccgcggac gggggaacgg tcgtggtgct ctgcggcgcg
     9121 ctcgccgatt cgcccgaccg ctccgagtgg ctggtccgcg cgctcgcccg gcgccaccgc
     9181 gtggtgacct accaccgggc cggccaggcg ggcagcaccg ccgcgccccg gaccggcccg
     9241 cacgccggtc cggacgccgg tccggacgcc ggtcccggcc acggcctgga cgccgccgcg
     9301 gccgatctcg ccgacctggt gctgcacgtc gcgggcggcc gcccggcgtt cctcgtcggc
     9361 cacggcgtcg gcggcgtcat cgccgcctcg gccgcgaccc ggctggaggg ccgcctcgcc
     9421 ggcctcgtcc tggtggaccc gcaccacccc ggcgacgtcc cccgcccgga ccccgacgaa
     9481 ggacccggcg ggacggcggc ggacgacccc gcgggagccc cggaccagcg cgaggtgacc
     9541 gcgctgctcg accagatgac ggtctcgctg cggctgggcc tcgggcagtt gctgcgcgac
     9601 ccggactggc tgcgcctgct cccgccggac gtgcgcggcc tggcccgcgc gcactaccgc
     9661 gacgccgccg gctggagcgc ggcccgcggc gagtggcggg cgctgcgcgc gcgggccgcg
     9721 gcagcgcggc cgggcgagga cgcggtcccg gccgccgccg tgcccacctg cctagtcctg
     9781 tgcgagccgg gcggcgcccc gcccgacgcc aggcgggccc tccaggagca gtacgcgcgg
     9841 cgcgccccgc acgccgtcgt ccacgcggtg gacgcctccc gcgacgacgt cctcgcggcc
     9901 cgcacgtccg cccgcgcggt cgcggaactg gtcacggcgt tcgcggcggg gtccggcggc
     9961 cccggccgcg acggcaccga ccagcgggag gaggccgccg atgtccccgc tcagcggtga
    10021 gggtccggcc gcaggccgcg acctggtcgg agcggcggcg atcggggccc tcgcggcctg
    10081 gttcgccgcc acggtgctca gccagcaccc gcgcccggag ttcaaccgga tcgccgtcct
    10141 cgacggcacc ggcgccctca tccccaactg gcgcttcttc gcgcccgagc cggcccgcca
    10201 cgacttccac gtcctgttca ggaccgagga cgcgcggggc cgggccaccc cctggcagga
    10261 gggctccgcg atcaccccgc gccgtccgct gcacctggtg tggttcccca accgccgccg
    10321 ggagaaggcc ctctacgacc tgtgccacga actcgttgcg gtcctgggcc agttcgacgg
    10381 cgacgtcacc cgcacgcccg cctaccgcat gctgcgcaac tccgtggccc gcgccgtcag
    10441 cgggcttccc ggacggccgc cccgcggctt ccagttcgtg gtcgcccggc acaccggcca
    10501 cgacccgggg cacgacccgg actacctctt cgtgtcgccg tacatcccct acaccgcgga
    10561 cgcgccgtac gcctgagcgg ggcgcgcccg gcggcggcgt caggaggtgg tggcccagcc
    10621 gagcagggcc gtgtggggga gggacagcgc gccgctgtcg gtccggaact ccgccgccag
    10681 cacgtcgtac tgccgccgca cctccgccgc ggtgtccggg ggcagggagt gcaggatctg
    10741 gccgaccgtg gcgatccccg ccgccgggcc cgaccaccac acctccgggt cggccacgtg
    10801 gttccagctc agtgtcgcgc agcgcgcccc gaccagcccg gcggccgtca gcagcccggt
    10861 caggccctcc tcggtgcggg cgaagtcgtc ctcggcggcg agcgtcggga cggacgaggg
    10921 ccgggcggcg ccggccgcct ccagggcccg tcccagcagc gcctgcccgg gcgcgggcgg
    10981 cgcggaccag atggtgaccg cgacccgccc gccgggcccg gccacccggc gcagctcggc
    11041 gagcgcgtcc gccggccggc ccacgtggtt gaccacgaag ttcgccacca ccgcgtcgaa
    11101 ctcgccgtcg gcgaagggga gttccggcag catcgcgtgc tgcaccgcgg cctccggcac
    11161 cgcccgcgcc gcacgctcga ccatgctcgg ctccgcgtcc accgcgtgga cctccgcgcc
    11221 gcggcgcacc gccagcgccg ccgcggtgcc ggggccggtg ccgacgtcca gcacccgtac
    11281 gcccgccccc acgccggcgg cgtccagcag catgggcacg gtgtgcgcgc acagcagccc
    11341 gaagctccgc gcgtacgcct ccgcccgtcc ggcccacgcc tcgcgctcga acgcgtcgaa
    11401 gtcgcccacc gcacacctcc gtgccgtacc gaaccgttca ccgaccgcct gtagccgatc
    11461 actctagagg agagaaagga cactgcccgc cactcttgcc aggccgcgga ccgccgccgg
    11521 cggtggagtc gcccgggccg cgcccgtcca cccaactcac gtaccgagca cgggcgttcg
    11581 cgtcgaccga cggaaccgcc ggggcggggt cgggcgggac cgccgcgccc ggcaggcggc
    11641 gcggacggtc ccttcgtgcg gcgggccgga cggcgggtca ggaggccggc ccgacgccca
    11701 agcctgccgg cccggttcgg tccgatcggt gcggcccgcc ggtgccgccc gtcagttcgg
    11761 cacggccagc agcagggcga agcgggccgc cgcgtcggtc caccagtgcc ggacggtgaa
    11821 accgccggcg tgcagctcgg cggtcagcga ctcgcggcgg aacttgcagg acagttcggt
    11881 gcgcaggtcc tcgccggccg cgaggtcgac ggacaggtcg aggtcgggga tcttgacggt
    11941 ctgcgcgacg cgcgagcgca ggtgcatctc gatccgctcg tcctcggcgt tccagacggc
    12001 gacgtgctcg aaggcgtcgg ggtcgaagtc cgcgccgagc tcccggttca gcacgtgcag
    12061 cacgttcttg tcgaacgcgg cggtgacgcc ggccgcgtcg tcgtaggcgc ggaccagcac
    12121 cgcggggtcc ttcaccaggt cggcgccgag cagcagcacg tcgtcgctgc tcagcgagcg
    12181 ggtcagggtg cggtagaagg cggcgcgctg cggcccgtcg aagttgccga tggtgctgcc
    12241 gaggaaggcg agcagccgcg ggcccggctc gtcggagagc gggatgccgc cctcgaagtc
    12301 ggcgacggtg gcggagacct cgagccccgg gtagtcggag cagatggccc gcccggccgc
    12361 ttcgagcgcc gaggcgctga cgtccagggg cgcgtagcgg cgcagcgtgc cgcccgcggt
    12421 gagggcgtcg agcagcagcc gggtcttgcg tgaggagccg gagcccagct cgatcagggt
    12481 ggccgcgcgg gtcgccgcgg cgatctccgg cgcccggcgg accaggatct cctgctcggc
    12541 ccgggtcggg tagtactcgg gcagccgggt gatctgctcg aacaggtcac tgccgcgctt
    12601 gtcgtagaac cacttgggcg gcagggtgcg cggagcggcg gtgaggccgc cgaccacgtc
    12661 ggcgtgcagc gtgtcggtga agtagccggc cggcagccgg tcgtcgaggg tgaagcggct
    12721 ctggttctcg tcgctcatgg gggtggtgtc cttgcgttcg gggcgcagcg cgatgacgcg
    12781 cacgccttcc gtggtggcca ggagcaggga acggtcgggg acctcgcgcc actcgccgag
    12841 cgcggccccg accgcgtcgt cgggttctga cgcgacgacc accgcacccg ggcgggcccg
    12901 gtaccagagc gtgtcgcccc acgcgctggc ggcgatggta cggccgtcgg tgagcaggaa
    12961 gttgagccga gcggtgggcc gcgcggcggc gacctcgcgg accacggcgg cgatcgcgcc
    13021 ccccggcggc tcgccccggc gcagccggcc cgcgagcatc gcccacagca gggcggagtc
    13081 cgaccgtgcc tccagggaca gcagttcggc ggagccgagc ccggtcggca gccgggtcca
    13141 gtccggcacc gcgccgttgt ggctgaacag ccagcggccg tcccggaagg gcgcggccgc
    13201 cgactcgtcg ggcgcggtgc cctcggtggc cgaccgcacc gcggccagga cggcgccgct
    13261 gcggatcacc cgggccagct cggcgaagtt ggggtcggcc cacaccggca gcgcccggcg
    13321 gtaccgcgcg ggcacggccg ccccttcgtc accgggccgt tccgcggggg cggccgggcc
    13381 gcccgccgcg gggacgggtg gcgccgggtc gtgcggggcc gggtcatgcg gggccgggcc
    13441 atgcggcgcc ggcggaccgg gcaccgcggc ggccccgggc tccccgggca ccgcggcttc
    13501 cgagtccgcc gggtaccaac ccaccccgaa accgtcggcg ttcaccgtgc cgtgccgctg
    13561 ccggcgcggc tgccaggact gctcgtacag gccccgcggc ggctccacga gcagccgcgc
    13621 cagcggcaac ggcgggccca cgtaggcgag atggcggcac atcagacgat cccaccgccc
    13681 gtcgcccccg aacccccggc gcgcgaggcg ccgttcgcga tcgcggcggt gccgggcgcg
    13741 gtgcgcgcct cggtgtccgt caggtccctc gccgtgcgga acccggcgaa gatctgccgt
    13801 cggatcgggt agtcccagtt ccggaaggtg ccgcggcagg ccaccgggtc caccccgaag
    13861 gcaccgccgc gcagcacctt gtactcgggg ccgaagaaca cctcggagta ctccttgtag
    13921 gggaaggcgg cgaagcccgg gtacggcgcg aagtccgagg acgtccactc ccagacgtcg
    13981 ccgatgagct gccgggcgcc gctgggcgcc gcgccttcgg gatagctgcc cgcggcggcc
    14041 ggccgcaggt gccgctggcc gaggttggcg tgctccgggc cggggtccgc gtcgccccac
    14101 gggtagcggc gcgagcggcc ggacgccggg tcgaaccgcg ccgccttctc ccactccgcc
    14161 tcggtgggca gccgccggcc ggcccaccgg gcgtaggcgt cggcttcgta ccagcacacg
    14221 tgcaccaccg gctcgtcggc cggcaccgcc tccacgtggc cgaacctgcg gcggatccag
    14281 cggtcgccgt cccgggacca gaacaggggc gccgtgagcg cggcgtcctg gacgtgccgc
    14341 cagccctccg gcgtccacca ccgcgggtcg cggtagccgc cggcgtcgat gaagtcggcg
    14401 tagcgcccgt tggtgaccgg caccgtgtcg agccagaacg gggccacccg cacctcgtgt
    14461 gcgggcagtt cgttgtccag cgcccacggc tcggtgctgg tgcccatcgt gaacgggccg
    14521 ccggggacga ggacctcgcg cggcagcccg gccgcgtcgg cgggcgcggg cggcggcgcg
    14581 ggggcgtcga ggacggccgg ccccgaccgc aactggtggg tggcgagcat cgtctcgccg
    14641 tgctgcgcct cgtgctgggc gagcatcccg aagacgaagc cgcccttcag cagccgttcg
    14701 gggtgcgcgg gatcggtgtc gatgccggac aggacgtcca gggtctcctc ccgcacctcg
    14761 gcgacgtagg cgcgcgcctg cgccggcccg agcagcggca gggcggggcg gtccgcgcgg
    14821 gggtgccgga acgcgtcgta gaggtggtcg aggtcgggcc gcacgccgtc gcgccggccg
    14881 gcctcgcgga ccagccacag ctcctcctgg ttgccgatgt gcgccaggtc ccacaccagc
    14941 ggggacatca gccgggagtg ctgggcgacc aggtcgtcgt ccgtgacgga atcggtgagc
    15001 agcccggtac ggtgccgggc ggcccgcagc gccgccagcg cgcgggcggc ggggtcgccg
    15061 ccggggtggt cgggacggcc ggggccgccg gaaggggccg cgggcgcgcg gccggccggg
    15121 gccgccggtg cgacgtgggg tgagggtgtg gccgagggcg tcgaagaggc tggagtggcc
    15181 ggattttcgg cagtgtcggg gtgcggggtg cgagggtctg tcacgactag ccttccggag
    15241 ggtcaggcgc ggctcaacgc gtccaactgg tcgtgggccg gacatcggcc gcgttgtgga
    15301 tagcgctcgg cgaagtcgga gacggcctgc cgcagggccg ggggagcccc ggtgcgcgcg
    15361 agggcgctct cggcggcggc gaagcaggcg cgggcggccc gggccagttc gggatcggcc
    15421 agaccggtcc gcgcggcacg gcgccagacg agatcggtgg ggaggggatc accgctcgtg
    15481 gcgagcggtt cggtggcggc gtacgcggtt tcggcggcga ccggatcgtc caacagggtt
    15541 gctgcgagca cggtcggcac gatccactgg tcgccgtcct gcgcgtcggt catccgcagc
    15601 tccagccagc cgcgcggccg gaccgggggg aagagggtgc tgaggtggta gtccaggtcg
    15661 gcgacggtgg gccgccggac cccgggggcg ccgcgcaccc actggcggaa ggtgagcccg
    15721 tggggcgcgg accagtcgcc ggaatcggtg cggtccgggc cgcttggaga gccgacaaga
    15781 ctgcctttga gagagcctgt ggcagagctt ccgcggccgg gagcagcgga ggcggcggtg
    15841 cggccgccgg cggaggacgc gccgggacgc gtggcgtgct ccgcggcagg gcccacggtg
    15901 ccgccgtcct ggcggatgca cagcacccgg gcgtccaggg cgtaccgcgc ccacgcggcg
    15961 cgcgggtcct cgtccggtcc gtcgtgccgc ggctgccggg tcctgccggg gtccatcagg
    16021 gcccagttcg cctgccgggt ggagacccag ccggtcggtc tgccgtcccg gaagggcgag
    16081 ttcgcgaacg ccgcgaccat caccgggccg atccggtggg ccagcgccca gcggccgcgg
    16141 tagccggtca cgcccgggcg gtcctcgccg ctgtccaggt tgacctgcaa ggacgcggtg
    16201 cggcgcatca tcgtgcgacc ccaggggccc tcgcggtcga agaagcgctc catcgcccgg
    16261 tagcggggat ggtcgaggac ccggggaggt tcgcggtggg ggtcgaggcc gtgtccgacg
    16321 agcaccagtc cggcgcgttc gacggcctcc cggagagcgg cgaggtcttc cgccatcgcc
    16381 gccacgcacc ccgcgaggct gggtgcgggt ggagagctca gctccacctg gccacccggt
    16441 tcccgggtga gcctgctgcc ccggggcagt gcccctgggg cctcgaccgg cgcgagcgcc
    16501 gcgtcgagtc gttcgacggg tatcagtgcg cggggatcgg agcggtcctg gaccagccac
    16561 tccagttcca cgccggtgcg ctcgggcggg cccgtcttga agcacacccc gtgtacgtgg
    16621 gcctccgcct cgtcctcggt gaggtcttca cgtcggtcgg cacggttgac agccatcggt
    16681 ccacctcctc atttcttctg tcgggtgttc atccgaagcg aggacatgtc acatgttgct
    16741 ggggagagtg tagttcgcca cggtgcgtgg cggtcttacg tcatgatgac cgggcatgcg
    16801 ggcaagcatt cgatatcggc cgagtattcc cccgtccgta ctcttcactc tttcgagtgg
    16861 gtcatatatg aataaaccag gtgatcgtga tgatacgggc ggtggtcgtc cgcagctcgt
    16921 cgcagggcga tgccccggcg gtgatcaaca cgtgaccggc taccctgccg gtaggagcag
    16981 tcgcaggtcc ccgtccccgg acggtccccg cggcggttcc cggtagtagc cgccgtagcc
    17041 gcccgaagcg cagaccccgc cagatgccag accagcgaca ggagtacccc tcgtgaccgt
    17101 cgtcgggccc ttcgggctga gcgtgtggga cgagaacctc gatgccgacg tccaggccgg
    17161 tctcgcagcc gtggaggaag ggctgctgga ggcgaccaag agcgacgtcc ccttcatcac
    17221 cgacacggcg cgccacctcc tgcgggccgg cggcaagcgc ttccggccgc tcctggtgat
    17281 gctcggggcg cagttcggcg acccgcacgc gccgggggtg gtgcccgccg ccgtcgtcgt
    17341 ggagctgacc cacctcgcca ccctctacca cgacgacgtc atggacgagg cggacgtgcg
    17401 ccgcggggtg ccgagcgcca actcccgctg ggacaactcg gtggcggtcc tcaccggcga
    17461 cttcctcttc tcccgggcct cccacatcct cgcggacctc gggcccgagg cggtgcgggt
    17521 gcaggccgag gcgttcgagc ggttggtcac cgggcagatc atggagaccg tcggcccgcg
    17581 cgacgggcgc gacccgctcg accactacct ggaggtcatc gcgggcaaga ccagctcgct
    17641 gatggcggtg tccggccggt tcggggcgat gatgtcgggc gccgacgagt cggtggtcca
    17701 cgtcctcacc cagtacggcg agcggatcgg cgtggcgttc caactggccg acgacgtgct
    17761 ggacatcgcg agcgacagcc acgagtcggg caagacgccc gggacggacc tgcgcgaggg
    17821 catcccgacg ctgccggtcc tgcacgtgcg ccgctggcgc gcgcagggaa agggcccggc
    17881 gacgcccgag gaccggcggc tgaacgaact cctcgacggc gacctgacgg acgacgcgct
    17941 ccacgcggag gcgctgcacc tgctgcgggc gcatcccgcg ctggagcagg cgcgccgtga
    18001 caccgtccgc tacgcggacg aggcgagggc ggcgctcgcg ccgctgcccg acctcccggc
    18061 gaaggcgtcc ctggccgccc tgtgcgaagc ggtcgtccac cgcgcgggtt gaggcggagc
    18121 gagggcgcct cacccccggg gtggtccgtg ccgtcggttc cgcgaccacc tgccggtggg
    18181 tcgccggccg caccgtatcc ccgcccctgg gcaaccgcgg gtcttccacg gccttccacg
    18241 gccggctgtc ttcggggcgg cctggtgggg ccagttggcg gggccgggag gggcaccggc
    18301 ggagcgtccg cgggagggcc cgcacggccc aggggcgcgg gggcggcgcg atcggccacg
    18361 acgcaccggg tggtcccgga accgacggca ccgcgtaccc cggagggtcg ggggcgcccc
    18421 gggcaggcgt catacccggg gcgtacggcg ggcccgcccc cggagggatg tgcgggggcg
    18481 gggaatctgg tcggatgggg tcaccccacc gcatggcgga ctgggggaca atcgccccgg
    18541 gtagggggaa accgcgagcg ggccgccgct gacgacggag gtacaacgcc atggcagtga
    18601 ccgaacggat cgggcgaccc gagccggagg agccggagcg accgcgcggc cgcgcccgga
    18661 agatcgcgcg ctacgccgta cccgtcgctg tggcgggcgt ggcggccgcc acgatcacgc
    18721 tggtgcccgc gctcgccgac gccggcgacc cctcgctgcc gaagatcacc gcacagcagt
    18781 tgctgacgaa gatcgcggcc tcggacaccc gcgccctgga cggcaccgtg aaggtgagca
    18841 ccgacctcgg cctgcccgac gcgctctcgg ggggcgcggc cggcggtctg ctgtcgggcg
    18901 cgggcggctc cggcaaggac ggctcctccg ccgccccgca ggcgcagctc gcccagctcc
    18961 tcgtcggcac ccaccagttg cacgtcgcgg tcgacggcca ggaccggcag aaggtctcgg
    19021 tcatcgagcc cgccgccgag tacagcgtca tccacgacgg cacccagttg tgggcgtacg
    19081 acagcgcgtc gaaccaggcg taccacgcga cgctgcccgc cgcggcggac gagtcctcgg
    19141 gcccggtcgg cgcgcggccg tccgacggcg tgccgaccac cccgcaggcc gccgccgagc
    19201 aggtgctccg ggcggcgcgc ggcacctcct cggtcaccgt cgacggcacc gcccgggtgg
    19261 ccggccgcag cgcctacgaa ctggtggtca cgcccgagca cgccgccgac accacggtcg
    19321 ggtcggtccg catcgcggtc gactccggca ccggggtccc gctgaagttc accgtgcttc
    19381 cgaagggcgg cggcaaggcc gtcttcgaga tcggcttcac caaggtcggg ttcggggagc
    19441 cggccgcgag caccttctcg ttcaccgcgc ccaagggcgc cgaggtgacc gagggcaagt
    19501 cctccggcgc gtccggcggc agcggggccg gcgccgtggc cggcctggcc gcgcccaccg
    19561 tgctcggcac cggctgggac tccgtcgcgg tgatcgacac cggcgacgcg ggctcggggt
    19621 cgggcgacgg cggcggcagc ggcttcggcg cggacggcac ggccggttcg ggccgcggcg
    19681 gcgacgcgtc gtcggcgctc gacggatacg gcaccaaggt caccggctcc ttcggcaccg
    19741 gcaccgtctt ccacacccgc ctggtgaacg tgctcctcac cgacgagggc aggctctacg
    19801 tcggcgcggt caccgagtcc gcgctgaccg cggcggccgg cggccaccac tagccccggt
    19861 gcgcggcgcg gcgcggtgcg gcagcgcggg agagcggcgg agccaggaga gcgagggagc
    19921 gcgatgacgg agccggcccc ggcggcggcc cccggtgagg ccgtgatcga gacccgcggc
    19981 ctcgccaagc ggtaccgcgg caccctcgcc gtcgacggcc tcgacctgcg ggtgccgcgc
    20041 ggcagcgtct tcggcttcct cgggccgaac ggctcgggca agaccaccac catccggatg
    20101 ctgatgggcc tggtcgagcc gaccgccggt accgcccggg tgctcggccg cccgatgccg
    20161 cgttcggccc gctcggtgct gccccgggtc ggcgcgctca tcgagggccc ggcgctgtac
    20221 ggcttcctgt ccggccgcga caacctgctg cggtacgacg cggccgaccc gaccgccgac
    20281 ccgcgcaccc gccgggcccg ggtcggcgcc gccctggacc gggtggggct ggccgcggcc
    20341 gcgcagaaac gggcccggca gtactcgctc ggcatgaagc agcgcctcgg cctcgcggcg
    20401 gcgctgctcc agccgcggga cctactggtg ctcgacgagc cgacgaacgg cctcgacccg
    20461 cagggcatgc gggagatccg caccctggtc agggagctgg ccgccgacgg cacgaccgtc
    20521 ttcctctcct cgcacctgct ggacgagatc gagcaggtct gcacgcacgc cgcggt
//
