LOCUS       NZ_AP018365            10429 bp    DNA     linear   CON 21-MAY-2024
DEFINITION  Streptomyces sp. SN-593 chromosome.
ACCESSION   NZ_AP018365
VERSION     NZ_AP018365.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. SN-593
  ORGANISM  Streptomyces sp. SN-593
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Panthee,S., Kito,N., Hayashi,T., Shimizu,T., Ishikawa,J.,
            Hamamoto,H., Osada,H. and Takahashi,S.
  TITLE     beta-carboline chemical signals induce reveromycin production
            through a LuxR family regulator in Streptomyces sp. SN-593
  JOURNAL   Sci Rep 10 (1), 10230 (2020)
   PUBMED   32576869
  REMARK    DOI:10.1038/s41598-020-66974-y Publication Status: Online-Only
REFERENCE   2
  AUTHORS   Takahashi,S., Toyoda,A., Sekiyama,Y., Takagi,H., Nogawa,T.,
            Uramoto,M., Suzuki,R., Koshino,H., Kumano,T., Panthee,S., Dairi,T.,
            Ishikawa,J., Ikeda,H., Sakaki,Y. and Osada,H.
  TITLE     Reveromycin A biosynthesis uses RevG and RevJ for stereospecific
            spiroacetal formation
  JOURNAL   Nat Chem Biol 7 (7), 461-468 (2011)
   PUBMED   21642985
  REMARK    Publication Status: Online-Only
REFERENCE   3
  AUTHORS   Panthee,S., Takahashi,S., Takagi,H., Nogawa,T., Oowada,E.,
            Uramoto,M. and Osada,H.
  TITLE     Furaquinocins I and J: novel polyketide isoprenoid hybrid compounds
            from Streptomyces reveromyceticus SN-593
  JOURNAL   J. Antibiot. 64, 509-513 (2011)
REFERENCE   4
  AUTHORS   Takahashi,S., Takagi,H., Toyoda,A., Uramoto,M., Nogawa,T., Ueki,M.,
            Sakaki,Y. and Osada,H.
  TITLE     Biochemical characterization of a novel indole prenyltransferase
            from Streptomyces sp. SN-593
  JOURNAL   J Bacteriol 192 (11), 2839-2851 (2010)
   PUBMED   20348259
REFERENCE   5  (bases 1 to 8833490)
  AUTHORS   Takahashi,S., Ueki,M., Toyoda,A., Ishikawa,J., Sakaki,Y. and
            Osada,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2017) Contact:Shunji Takahashi Chemical Biology
            Research Group, RIKEN Center for Sustainable Resource Science
            (CSRS); Hirosawa 2-1, Wako, Saitama 351-0198, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Phrap v. 0.990329
            Genome Coverage                   :: 16x
            Sequencing Technology             :: ABI 3730
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016756395.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 03:58:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,420
            CDSs (total)                      :: 7,334
            Genes (coding)                    :: 7,181
            CDSs (with protein)               :: 7,181
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 35 of 153
            Pseudo Genes (incomplete)         :: 136 of 153
            Pseudo Genes (internal stop)      :: 8 of 153
            Pseudo Genes (multiple problems)  :: 25 of 153
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:29:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4108973
            Orig. end                         :: 4119402
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP018365.1.
            This work was done in collaboration with Shunji Takahashi, Masashi
            Ueki, Atsushi Toyoda, Yoshiyuki Sakaki and Hiroyuki Osada (RIKEN)
            and Jun Ishikawa (National Institute of Infectious Diseases).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10429
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="21"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10429
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10429
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5429](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5429
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    98..112
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            1043..1783
                     /locus_tag="RVR_RS17180"
                     /old_locus_tag="RVR_4805"
     CDS             1043..1783
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325471.1"
                     /locus_tag="RVR_RS17180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_4805"
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_202234698.1"
                     /transl_table=11
                     /translation="MRRTVKHPDDISDPQDRGRRAPRRPLPRWLPLPLGIGIGLLGGVG
                     YGLTATPQYTATSYVMVVPRQGTDAQTALGFAQAYGRIVTGSAVLAGAQAATHSAAADL
                     KGHVQAATSPDAPMISVTGSSSEAGTAAAVANGVARSLAAVGNRDADSTGVRLLVFAPA
                     AAPAAPSSPSAALSGAVGASAGGLLGALTLLVRPRRQDGAGRPDRAQVPAPAASPPASE
                     PAAPSPESSQASEPAASRPERTAV"
     gene            1912..3192
                     /locus_tag="RVR_RS37650"
                     /old_locus_tag="RVR_4806"
     CDS             1912..3192
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /locus_tag="RVR_RS37650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_4806"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_237405328.1"
                     /transl_table=11
                     /translation="MRICRDPEEFAALAPEWTRLHRSCPQATPFQSHAWLHSWWLSYGR
                     PGRLRVVLVRRGGDGTQGGDGTGTLVGAVALMRVHRPLPALVPLGGAVTDYSDVLIDPA
                     ADAYPDADAPARSHSASDGRDESGEGPAADPVGPGNAGSRRDAGNTGNAGSAGSAGSAC
                     GGPVAAALVRGMRRAARGGVVDLREVPADAAAERVFALWPGPRRRLADSECLELPGGPM
                     AGLIARVGSSRGQRIRGHLRKIDASGVEERDVPAAEVPDAIRTILRLHTAQWAGRGVTP
                     EHVRPRFAEHLGRAGAAMTASGEALLTEFRIGGEVVAANLTVLSPTLAGGYLYGADFAA
                     LRAAKVDLNTLLMRHGARLTTAADRPVLSMLRGAEPHKAHWRPVPTTNARLLLGTRRAL
                     PSLLCRQVLAAARSRLREVRRRARAAT"
     gene            complement(3215..4402)
                     /locus_tag="RVR_RS17195"
                     /old_locus_tag="RVR_4807"
     CDS             complement(3215..4402)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018568401.1"
                     /locus_tag="RVR_RS17195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_4807"
                     /product="glycosyl hydrolase"
                     /protein_id="WP_237405329.1"
                     /transl_table=11
                     /translation="MGAFTDSGAEGVRNLAALQTWLGGTQVSVGHTYLPGDDWGSIEGD
                     GDLLQPWADWTRAEPGRLFVLNVPMQADNEGGLPDSQVRDLIAQGASGAFDAHFTALAQ
                     RLVRLGVPDTVIVLGWEMNGTTYTHRCGPDPEGWKAYWNRVVTAMRAVPGQHFRFDFAP
                     DRGRDAVAWTQCYPGDATVDVIGMDSYDQPSGETFHQQVTEPYGLQAQVDFAAAHGKEI
                     SYPEWGLFRNGDDADYMSLMLTWIITHHPLYQTITDYCPHGVLQCAQNPEASKVYRALL
                     FGRRTATAGPVGTAPAAPAPGTGTDGAGGPGTGTSTTSSPVETCVPMPLSEVLKKSYTS
                     GQVCMRLEPRAAAPATPAAPATPSATPSAGPSATPSARPSAAPTVTPTPGPTSAL"
     misc_feature    4654..4668
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.25"
                     /tool="antismash"
     misc_feature    complement(4791..4804)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.92"
                     /tool="antismash"
     misc_feature    4900..4914
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 13.61"
                     /tool="antismash"
     misc_feature    complement(4922..4936)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.81"
                     /tool="antismash"
     gene            5001..5429
                     /locus_tag="RVR_RS17200"
                     /old_locus_tag="RVR_4808"
     CDS             5001..5429
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129287.1"
                     /locus_tag="RVR_RS17200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_4808"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_237404800.1"
                     /sec_met_domain="melC (E-value: 3.2e-30, bitscore: 103.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASRRRVLRTGLISAAAVTGTAAGLGPMLAADRSSPRAATGPGAG
                     GQDAKAVAGADLEDVSLPLFDEVYRGRHIQGFTSDDPSGLLLMVDGRPLGAMRRFDGSY
                     VSMANHFQPYPTPLSTARGAVDVIGNAELGDAAAVHHH"
     gene            5457..6326
                     /locus_tag="RVR_RS17205"
                     /old_locus_tag="RVR_4810"
     CDS             5457..6326
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267933.1"
                     /locus_tag="RVR_RS17205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_4810"
                     /product="tyrosinase family protein"
                     /protein_id="WP_202234701.1"
                     /transl_table=11
                     /translation="MYTRKNQRNLTSDQRSRFVQAVLQLKRRGGYDKYVKLHTQYFSAD
                     GETGLRVAHMAPTFFPWHRQFLLVFEHELRMIDPDVSIPYWDWTADRSTTSALWDDDFM
                     GGNGRSSDGQVMTGPFAYGSGDWPITYGVTRERYLTRNMGRPDRPIVLPTAAELNDALK
                     IPTYDTAPWNSAPGTQGLRNKMEGWVVSDTKGGYLHNRVHQWVGGHMIGGSSPNDPVFW
                     LHHAFIDLIWTRWQRLHPKSGFLPAKPLSAKDPEHGRVISLDEPMPPFHVKPSAVLDAR
                     GYYTYEEG"
     gene            complement(6416..6697)
                     /locus_tag="RVR_RS17210"
                     /old_locus_tag="RVR_4811"
     CDS             complement(6416..6697)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018845748.1"
                     /locus_tag="RVR_RS17210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_4811"
                     /product="chaplin"
                     /protein_id="WP_202234702.1"
                     /transl_table=11
                     /translation="MSRIAKAAALTAAAGAALAAGAGVASADAGAGAAAVGSPGVVSGN
                     VVQVPVHVPVNVCGNTVDVVGLLNPVFGNQCENSSSHGWDGNGAGAQY"
     gene            complement(6963..7652)
                     /locus_tag="RVR_RS17215"
                     /old_locus_tag="RVR_4814"
     CDS             complement(6963..7652)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RVR_RS17215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="RVR_4814"
                     /product="hypothetical protein"
                     /protein_id="WP_202234703.1"
                     /transl_table=11
                     /translation="MLSHRTTGVAVSAALVAALAGGVVGTASADAPHQHSAAAQPATPA
                     AQPAARADAATVLAQAKTLGGLGSVLTPVTTLVHDALATPVSKDKVASDATAATAAIAA
                     AKKDLPATPATPTAPSAPNGSAASNAPAAPSGSAAATGAVRPRAAADVQADALAALQTA
                     IDSLSKAVAAGDAAGAVTAGTAVVTGLVNVAVATVLGGGLPQPDLPGLPTLPSLPSTPT
                     LPTTPGL"
     misc_feature    7947..7960
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.67"
                     /tool="antismash"
     gene            7947..8447
                     /locus_tag="RVR_RS17220"
                     /old_locus_tag="RVR_4813"
     CDS             7947..8447
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984773.1"
                     /locus_tag="RVR_RS17220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_4813"
                     /product="DUF5949 family protein"
                     /protein_id="WP_202234704.1"
                     /transl_table=11
                     /translation="MSQTNTASASSLQSRLGTLVVIPWAGSHEDGGDTPFLLAYSLGDG
                     VDGPEAGQEAVLQAAKEIGLPVGGAILDVAQSPKADVRVLVEGGKAVLTMPYLKVTCPV
                     PDQWTAAARARESVYVILASRPWPEAAPGTAVTEESLRAFAADAEVLGTAAHFTVPVGT
                     LRV"
     gene            complement(8523..8786)
                     /locus_tag="RVR_RS17225"
                     /old_locus_tag="RVR_4815"
     CDS             complement(8523..8786)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RVR_RS17225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="RVR_4815"
                     /product="hypothetical protein"
                     /protein_id="WP_202234705.1"
                     /transl_table=11
                     /translation="MNVFRILTTAAAGSFVLAAAAPAVQAADLSSTLASTAVTAADTGA
                     QAGSVASGVVQQSGVGSKVDAVQKAVKAGSDAVTAGNELVNG"
     misc_feature    complement(8839..8852)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.61"
                     /tool="antismash"
     misc_feature    8929..8946
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 13.84"
                     /tool="antismash"
     gene            9042..10076
                     /locus_tag="RVR_RS17230"
                     /old_locus_tag="RVR_4816"
     CDS             9042..10076
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136820.1"
                     /locus_tag="RVR_RS17230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_4816"
                     /product="PD40 domain-containing protein"
                     /protein_id="WP_202234706.1"
                     /transl_table=11
                     /translation="MHATTSRRTRGRRRSTAAAIALTAGITASAALLLTGCGPDDPTAD
                     GTAPPSASGGPTGGSGGTTVPADPGGTRLNGTAHNGLTLSDGSRYVVMNGTRVDFGTLV
                     RDLAWSPDGGRAAFVDGDGDLAVSGADGSGRVVVARNPGGQTWSHPTWQTRTADPANGM
                     TALDNLVFAVRSGGTTTLESVPSAGGTPQPITLNPMAVEHPDPLPTTGNTWPNAGREGT
                     AVYANTDSGQVYLRDDNLRQQGSALTAGSEPAMSPGDDEDVVFVRSVDGHDHLFLEDST
                     DGGPTYKDLTPDATTDYTEPAFSPDGTLIAARTRSGTVELRADGSGTPVKISDYTGLPA
                     FRSS"
ORIGIN
        1 gggtgcgcac cgtcgtggcc accgagcact cgctgggcgc cacccagatc gagggccgcc
       61 cgctgcgcct gggcacgcgc gccctgtacc ggagcaccga gcggctcggc cacggcaccc
      121 tcgcggtgtc cgcgaccgtc gccgaacggc tggtgtactg gggggtgccg gaggcgcggg
      181 tgcacctcgc gcccaacggc atcgagcccg aacgcttccg cttcgacccc gaggtgcgcg
      241 ccgaggtgcg gacccggctg cgcatccccg cgggcgcgta cgtggtcggc ggggtggggc
      301 ggcaggtgcc cggcaagcgg ttcgacgcgc tgatccgggc gatcgccgag atccccgacg
      361 tgcacctgct gctcgccggc gacgggcccg aacgggtgcc gctgctgcgg ctcgtgcacc
      421 acctgaacct ccagcaccgg gtgcacgtca ccgactccgc cggcgcggac gtgcccgggc
      481 tgctcgccgc gatggacctg ttcgtctcgg tctccgccga ggaggcgttc gggctcgcgg
      541 tggtcgaggc gctggccgcc gggctgcccg cgctctacac ggcctgcccc gcggtggagg
      601 acgtgccggc cgaggacgcg cccggcgcct gccggatcga cctgggcgag ctgcccgccg
      661 cgctggccgc ggagcaccgc gcggggccgc gccggctgcc cgcccccgcg gccgtcgagt
      721 actaccacat cgcgcggacc gcgcagcgcg tcatggccct gtacacccag gcgtccgagc
      781 gggagagcct gcgggagaac ctccgggagg gcctgcggcg ccgccgccgg tccgcggcca
      841 ccgcgatcgt gccccgaccg gcgcgcgccg ccgcgccccg cccggcgtcg gcggccccgc
      901 ccgcggcccc gcccgcgcag gcggccgcgc cgtcgtcccg gcagggggcc gtccagccgc
      961 ccgcgccgcc ggcgtcccgg caggagtccg ggaccgtccc cggcgccgcc gccgaaccca
     1021 ccaccccgac acccgcccag aagtgagacg caccgtgaag caccccgacg acatcagcga
     1081 cccgcaggac cgcgggcgcc gcgccccgcg acggccgctg ccgcgctggc tgccgctgcc
     1141 gctcggcatc ggcatcggcc tgctcggcgg tgtcgggtac ggcctgacgg ccaccccgca
     1201 gtacaccgcg accagttacg tgatggtcgt accgcggcag ggcaccgacg cgcagaccgc
     1261 gctcggcttc gcccaggcgt acggccggat cgtcaccggc tctgcggtgc tggccggggc
     1321 gcaggccgcc acccactccg ccgccgccga cctgaagggc cacgtccaag cggcgacgtc
     1381 gcccgacgcc ccgatgatct cggtcaccgg cagttcgtcg gaggccggca ccgccgccgc
     1441 cgtcgccaac ggcgtggccc gctcgctcgc cgccgtcggc aaccgcgacg cggacagcac
     1501 cggcgtacgg ctgctggtct tcgctcccgc ggccgccccc gccgcgccgt cctccccgtc
     1561 ggcggcgctg tccggtgcgg tcggcgcgag cgcgggcggg ctgctcggcg cgctcaccct
     1621 gctggtccgc ccccgccggc aggacggcgc cgggcggccg gaccgcgccc aggtccccgc
     1681 tcccgccgcc tccccgccgg cgtccgagcc ggccgcgccg tcgccggagt cctcgcaggc
     1741 gtccgagccg gccgcgtccc ggccggagag gacggcggtg tgacggtgga gggcccgaag
     1801 accgcggtcc cggccgccgc cggcagcccg ccgcgcccca ggaccgcacc gcgccgcccg
     1861 tccggggccg cttccccggc cccggcgggc gcgcccgcgg ccgcgctcga cgtgcggatc
     1921 tgccgcgacc ccgaggagtt cgccgcgctc gcccccgagt ggacccggct gcaccgcagt
     1981 tgcccgcagg ccacgccgtt ccagagccac gcgtggctgc actcgtggtg gctgtcgtac
     2041 ggtcgccctg ggcggctgcg ggtggtgctg gtcaggcgcg gcggggacgg cacgcagggc
     2101 ggcgacggga ccggcacgct ggtgggcgcg gtcgccctga tgcgcgtcca ccgcccgctg
     2161 cccgccctcg tgccgctcgg cggtgccgtc acggactact cggacgtgct catcgacccc
     2221 gcggccgacg cgtaccccga cgccgacgcg cccgcccggt cgcacagcgc ttccgacgga
     2281 agggacgagt cgggcgaggg ccctgcggcc gaccccgtgg gcccggggaa cgcggggagc
     2341 cggagggacg ccgggaacac ggggaacgcg gggagcgcag ggagcgcggg gagcgcgtgc
     2401 ggcggaccgg tggcggccgc cctcgtccgc gggatgcgcc gcgccgcccg gggcggggtc
     2461 gtcgacctgc gcgaggtgcc cgcggacgcc gccgccgagc gggtgttcgc gctgtggccc
     2521 ggccccaggc ggcggctggc ggactcggag tgcctggaac tgcccggcgg ccccatggcc
     2581 gggctgatcg cccgggtcgg cagctcacgc ggccagcgca tccgcggcca cctgcgcaag
     2641 atcgacgcct cgggcgtcga ggagcgtgac gtccccgccg ccgaggtacc cgacgcgatc
     2701 cgcacgatac tgcggctgca caccgcccag tgggcgggcc ggggcgtcac ccccgagcac
     2761 gtgcgccccc ggttcgccga gcacctcggc cgtgccgggg ccgcgatgac cgcctccggc
     2821 gaggccctgc tcaccgagtt ccggatcggc ggcgaggtgg tcgccgcgaa cctcaccgtc
     2881 ctgtcgccga ccctggccgg cggctacctc tacggcgccg acttcgccgc gctgcgcgcc
     2941 gcgaaggtgg acctcaacac cctcctgatg cggcacggcg cccgcctcac cacggccgcc
     3001 gaccgcccgg tcctgagcat gctgcggggc gccgagccgc acaaagccca ctggcgcccg
     3061 gtccccacga ccaacgcccg cctcctgctg ggcacccgcc gcgccctgcc ctccctgctg
     3121 tgccgccagg tcctggccgc cgcccggtcc cggctgcggg aggtccgccg ccgcgcgcgg
     3181 gccgcgacct gaccgccccc gccgcgcgcc cgcctcacaa ggcgctggtc ggccccgggg
     3241 tcggcgtcac cgtgggcgcg gcggagggcc tggcggacgg cgtcgcggaa ggcccggcgg
     3301 agggcgtggc ggaaggcgtg gccggggccg cgggtgtggc cggggcggcg gcccgcggtt
     3361 cgagccgcat gcacacctgc ccgctggtgt acgacttctt cagcacctcg ctgagcggca
     3421 tcggcacgca ggtctcgacc ggggaggacg tcgtcgacgt gccggtcccg gggccgccgg
     3481 cgccgtcggt gccggtaccc ggggccggtg cggcgggcgc ggtgccgacg ggcccggcgg
     3541 tcgccgtccg gcggccgaag agcagcgccc ggtacacctt cgacgcctcg gggttctgcg
     3601 cgcactgcag gacgccgtgc gggcagtagt cggtgatcgt ctggtacagc gggtggtggg
     3661 tgatgatcca ggtgagcatc aggctcatgt agtcggcgtc gtcgccgttc ctgaacaggc
     3721 cccactccgg gtaggagatc tccttgccgt gcgcggcggc gaagtccacc tgggcctgga
     3781 gtccgtacgg ctcggtgacc tgctggtgga aggtctcgcc ggacggctgg tcgtaggagt
     3841 ccatgccgat gacgtcgacg gtggcgtcac cggggtagca ctgcgtccag gcgaccgcgt
     3901 cccgcccgcg gtcgggggcg aagtcgaacc ggaagtgctg gccggggacc gcgcgcatag
     3961 cggtgaccac ccggttccag tacgccttcc agccttcggg gtcggggccg cagcggtggg
     4021 tgtaggtggt gccgttcatc tcccagccga gcacgatcac cgtgtcgggc acgccgagcc
     4081 ggaccaaccg ctgggcgagc gcggtgaagt gcgcgtcgaa ggcgccgctc gcgccctgcg
     4141 cgatgaggtc gcgcacctgg gagtcgggga gtccgccctc gttgtcggcc tgcatgggca
     4201 cgttgagcac gaagagccgg cccggttcgg cgcgggtcca gtcggcccac ggctggagca
     4261 ggtcgccgtc gccctcgatg ctgccccagt cgtcgcccgg caggtaggtg tggccgacgc
     4321 tcacctgggt gccgccgagc cacgtctgga gcgcggcgag gttccgcacg ccttcggcgc
     4381 cggaatcggt gaaggcgccc atcggcggca gcccgccgtt cgcctgcgtc accgtgcccg
     4441 gcgtggcgga gggcgcggcc gacgccgcgc cccggccgga cccggtggcg gtgtccgtgc
     4501 ccgccgggga gggcagcggg gccgcgacgg ggctcccgta cgccggcagg tcgagcagcc
     4561 ggccggcgga gtcgtcgccg aggccggtcg cgctcgcccc ggacagcagc gccgcggccg
     4621 tggccagcgc gatgcccgcc ctcacccacc agtggtgtcg ctcggccatg gcccctcctc
     4681 gttcgacggc gaaggcgcgc cggaagcgcc acctgaccat cagtcataac gtacgaataa
     4741 acggcggctg cggattcggc gcgccgggcg ccaccgcgcc acatacggat cgcatggtca
     4801 tacgatcggg ttctcctccg cgaaattccc tgccgtcaaa gggatttccc ctccgccacc
     4861 accggccgaa gttttccggt cacatcaccc ggtcggatga atttccagac cgctgttgcc
     4921 gatcgccgcc ggtaatcccg ggaatccggg ccccggcggg caagatgagc cgcgccgaga
     4981 gccgaggaga ggagccgggc atggcgtcac gcaggagggt gctcaggacc gggctgatca
     5041 gcgcggccgc cgtcacgggc accgcggccg ggctgggccc gatgctcgcg gcggaccggt
     5101 cgtccccgcg ggccgcgacc gggccgggcg ccggcgggca ggacgcgaag gccgtggcgg
     5161 gcgccgacct ggaggacgtg agcctcccgc tgttcgacga ggtctaccgg ggccggcaca
     5221 tccagggctt cacctcggac gaccccagcg gtctgctgct catggtcgac gggcggccgc
     5281 tgggcgccat gcggcggttc gacggcagct acgtcagcat ggccaaccac ttccagccgt
     5341 acccgacgcc gctgtccacc gcccggggcg cggtcgacgt catcgggaac gccgaactgg
     5401 gcgacgccgc cgccgtgcac caccactgac cgaccgagcg tcgaggggcc gcacctgtgt
     5461 acacccggaa gaaccagcgg aatctgacca gcgaccagcg ctcacgcttc gtgcaggccg
     5521 tactccagct caagcgccgc ggcgggtacg acaagtacgt caagctgcac acccagtact
     5581 tctccgccga cggcgagacg ggactgcggg tggcgcacat ggcgcccacc ttcttcccct
     5641 ggcaccgcca gttcctgctg gtcttcgagc acgaactgcg gatgatcgac cccgacgtca
     5701 gcatcccgta ctgggactgg accgccgacc ggtcaacgac gtcggcgctg tgggacgacg
     5761 acttcatggg cggcaacggc cgctccagcg acggccaggt gatgaccggc cccttcgcct
     5821 acgggtcggg cgactggccg atcacctacg gcgtcacgcg cgagaggtac ctgacccgca
     5881 acatgggccg cccggaccgg ccgatcgtgc tgcccaccgc cgcggagctg aacgacgcgc
     5941 tcaagatccc gacctacgac accgcgccgt ggaactccgc gcccggcacc caggggctgc
     6001 gcaacaagat ggaggggtgg gtggtcagcg acaccaaggg cggctacctc cacaaccggg
     6061 tgcaccagtg ggtcggcggc cacatgatcg gcgggtcctc gccgaacgac ccggtcttct
     6121 ggctgcacca cgccttcatc gacctgatct ggacccgctg gcagcggctg cacccgaagt
     6181 ccggcttcct gccggccaag ccgctcagcg cgaaggaccc cgagcacggc cgtgtgatca
     6241 gcctcgacga gccgatgccg cccttccacg tgaagccctc ggcggtgctg gacgcccgcg
     6301 ggtactacac gtacgaggag ggctgacgcg ggccgtcccg cgaccggcac ggcacgcgta
     6361 cggcccccgc ccgtagttct tctcgggcga gggccgtacg cgtggtgcgg gggactcagt
     6421 actgggcgcc ggcgccgttg ccgtcccagc cgtgcgagga gctgttctcg cactggttgc
     6481 cgaacaccgg gttgagcaga ccgacgacgt cgacggtgtt gccgcacacg ttcaccggaa
     6541 cgtgcacggg cacctggacg acgttgccgg acacgacacc cggcgaaccg accgcagcgg
     6601 ccccggcacc ggcgtcggcc gaagccacac ccgcacccgc ggccagcgcg gcacctgcgg
     6661 cggcggtgag ggccgcggcc ttcgcgatac gcgacatcaa gatctccctg agacgtcata
     6721 cgagatggga atcgccgcaa tgctgcggct tttgacgcct tgggcaacgg ccgggaccgg
     6781 gaatggtcac gcaggttggg ccgaatcggt gaggaatttc cacggacgcg cggaatcggg
     6841 ggcgggcggt tcggaaaccg cgcccgacgt ccgggcggac ccgcggcccg cggcccgttc
     6901 cgcacgggga ggaggccgcg ggccggggcg cggtcagccc ggcgggaggg ccgggctcgg
     6961 ggtcacagcc ccggagtggt gggcagcgtg ggggtggacg gcagcgaggg gagcgtcggc
     7021 aggcccggca ggtcgggctg gggcagcccg ccaccgagca ccgtggcgac cgcgacattg
     7081 accaggccgg tgacgaccgc ggtgccggcg gtgacggccc ccgccgcgtc gcccgcggcg
     7141 acggccttgc tcagcgagtc gatggcggtc tggagcgcgg ccagggcgtc cgcctgcacg
     7201 tcggccgcgg cgcgcggccg caccgccccg gtggcggccg ccgaaccgct cggcgcggcg
     7261 ggggcgttgg acgcggccga accgttcggc gcgctcggcg cggtcggggt cgccggggtc
     7321 gcgggcaggt ccttcttcgc cgcggcgatg gccgccgtcg cggcggtcgc gtccgaggcc
     7381 accttgtcct tggagacggg cgtggccagc gcgtcgtgga ccagcgtcgt gaccggggtg
     7441 aggacgctcc cgaggccgcc gagcgtcttg gcctgggcga gcaccgtggc cgcgtccgcc
     7501 cgcgccgcgg gctgggcggc gggagtcgcg ggctgggcgg cggcggagtg ctggtgcggg
     7561 gcgtcggcgg acgcggtgcc gacgacgccg ccggccaggg cggcgacgag cgcggcggag
     7621 acggccacgc cggtggtgcg gtggctgagc atggaacggt ttcccttcgt ggaagcggga
     7681 ctgctgcctc gaccacggtg ggaaggggtc cgcgggcccg caaccgagag ggccgggcgg
     7741 acccggcgga ccctgcccgc gtccccggca agtcggctgt gcgcgggcgg ggttcggcgg
     7801 tcacccgacc gccgggccgg gatactccgt cggggtgcga acgccgtgcc gacagctcca
     7861 cgaacgggcg aatcctgtgc ccgggtgtcg cccgctgagg tgacatcggc gcggtaccgg
     7921 gccggtagcc cgttagggtc cggcgcatga gccagaccaa caccgcctcc gcgtcgtccc
     7981 tccagagccg cctgggcacc ctcgtggtga tcccctgggc cggcagccac gaggacggcg
     8041 gggacacccc gttcctgctc gcctactccc tcggtgacgg ggtggacggc cccgaggccg
     8101 gacaggaggc ggtgctccag gcggccaagg agatcgggct gccggtgggc ggcgcgatcc
     8161 tggacgtggc gcagtccccg aaggcggacg tgcgggtgct ggtcgagggc ggcaaggcgg
     8221 tgctgacgat gccttacctg aaggtgacct gcccggtgcc ggaccagtgg acggccgcgg
     8281 cccgcgcccg cgagtcggtc tacgtcatcc tggccagccg cccctggccc gaggcggccc
     8341 cgggcaccgc ggtcaccgag gagtcgctgc gcgccttcgc cgccgacgcg gaggtgctcg
     8401 gcaccgcggc ccacttcacg gtcccggtcg gcaccctgcg ggtctgagcc ggaccggacc
     8461 gcggacagca ctgaggggcg cccccggaaa ggggcgcccc ttgtgcgtgc ggacgggagg
     8521 agtcagccgt tgacgagttc gttgcccgcg gtcaccgcgt cggacccggc cttgacggcc
     8581 ttctggacgg cgtcgacctt cgagccgacc ccggactgct ggaccacgcc ggacgcgacg
     8641 ctgccggcct gcgctccggt gtcggcggcg gtcaccgcgg tgctcgcgag ggtggagctg
     8701 aggtcggcgg cctgcacggc gggcgcggcg gcggcgagca cgaaggaacc ggccgcggcg
     8761 gtggtgagga tgcggaatac gttcatggtc cgtacaacgc ccgagcccgc tcccagggca
     8821 cgcccccggc gccgggcgtc agccccggtc cacggcgttc acccgtgcag gtggaacggc
     8881 gcacccgtcc ggcgcagccc cgccgacgag gtgaccgcac gtaccgcggt gttgcccggc
     8941 tgtgacaacc accgcacgcc gcggggacgt tggcttgcca ggcttccccc agcggctcgt
     9001 accgccaccg ccgcaccgtc ttcgttcatg ggggaaccga gatgcacgcg accacctccc
     9061 gccgtacccg cggccgcaga cgcagcaccg ccgccgccat cgcgctgacc gcggggatca
     9121 ccgccagcgc cgcgctgctg ctgacggggt gcggccccga cgacccgacc gccgacggca
     9181 ccgcgccgcc gtcggcctcc ggcggcccga cgggcgggtc cggcggcacg accgtccccg
     9241 cggaccccgg cggcacccgg ctcaacggca ccgcgcacaa cggcctcacc ctcagcgacg
     9301 gcagccgcta cgtcgtcatg aacggcaccc gcgtggactt cggcaccctg gtgcgcgacc
     9361 tcgcctggtc cccggacggc ggccgggccg cgttcgtcga cggcgacggc gacctcgcgg
     9421 tgagcggcgc cgacggctcc ggccgggtgg tcgtggccag gaaccccggc ggccagacct
     9481 ggtcgcaccc gacctggcag acccgcacgg ctgacccggc caacggcatg accgccctgg
     9541 acaacctcgt cttcgccgtg cgcagcggcg gcaccaccac gctggagagc gtcccctccg
     9601 ccggaggaac cccgcagccg atcaccctca acccgatggc ggtggagcac ccggacccgt
     9661 tgccgaccac cggcaacacc tggccgaacg ccggccggga gggcaccgcg gtctacgcca
     9721 acaccgacag cggccaggtc tacttgcgcg acgacaacct gcggcagcag ggctcggcgc
     9781 tgaccgcggg ctccgagccg gcgatgtccc cgggcgacga cgaggacgtc gtcttcgtcc
     9841 gctcggtcga cggccacgac cacctgttcc tggaggactc cacggacggc ggcccgacgt
     9901 acaaggacct caccccggac gccaccacgg actacaccga gccggcgttc tcgcccgacg
     9961 gcacgctgat cgccgcgcgc acccgcagcg gcacggtgga gctccgcgcc gacggctccg
    10021 gcacgccggt gaagatctcc gactacacgg ggctgccggc cttccggtcg tcgtagggcg
    10081 gccgcgtctc agcggcggtc gccgagttcg gcccgcaggg cggccaggtg ggtgtcggcg
    10141 ttctcgtgcg gcaggaactc caccacgtcc aggaagcgga agagggtccg gtccgcggtg
    10201 accgcgaact cgtactccgc gaagccgacc accgccccca gcaccccgcg cgcggcgccc
    10261 cgtacgacat gggcggtcag cgcgtcgggg tgctcggcgt cggtgccgcg gcggccgccc
    10321 gggcgggcca ggtacgggca gaccatcgac gcgtagagca tgcacgcccg gtgcccgggt
    10381 ccctcggtgg tcggcgcgag gttgcggtac gggcggcccg ccgcgcgcg
//
