LOCUS       NZ_AP018365            10918 bp    DNA     linear   CON 21-MAY-2024
DEFINITION  Streptomyces sp. SN-593 chromosome.
ACCESSION   NZ_AP018365
VERSION     NZ_AP018365.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. SN-593
  ORGANISM  Streptomyces sp. SN-593
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Panthee,S., Kito,N., Hayashi,T., Shimizu,T., Ishikawa,J.,
            Hamamoto,H., Osada,H. and Takahashi,S.
  TITLE     beta-carboline chemical signals induce reveromycin production
            through a LuxR family regulator in Streptomyces sp. SN-593
  JOURNAL   Sci Rep 10 (1), 10230 (2020)
   PUBMED   32576869
  REMARK    DOI:10.1038/s41598-020-66974-y Publication Status: Online-Only
REFERENCE   2
  AUTHORS   Takahashi,S., Toyoda,A., Sekiyama,Y., Takagi,H., Nogawa,T.,
            Uramoto,M., Suzuki,R., Koshino,H., Kumano,T., Panthee,S., Dairi,T.,
            Ishikawa,J., Ikeda,H., Sakaki,Y. and Osada,H.
  TITLE     Reveromycin A biosynthesis uses RevG and RevJ for stereospecific
            spiroacetal formation
  JOURNAL   Nat Chem Biol 7 (7), 461-468 (2011)
   PUBMED   21642985
  REMARK    Publication Status: Online-Only
REFERENCE   3
  AUTHORS   Panthee,S., Takahashi,S., Takagi,H., Nogawa,T., Oowada,E.,
            Uramoto,M. and Osada,H.
  TITLE     Furaquinocins I and J: novel polyketide isoprenoid hybrid compounds
            from Streptomyces reveromyceticus SN-593
  JOURNAL   J. Antibiot. 64, 509-513 (2011)
REFERENCE   4
  AUTHORS   Takahashi,S., Takagi,H., Toyoda,A., Uramoto,M., Nogawa,T., Ueki,M.,
            Sakaki,Y. and Osada,H.
  TITLE     Biochemical characterization of a novel indole prenyltransferase
            from Streptomyces sp. SN-593
  JOURNAL   J Bacteriol 192 (11), 2839-2851 (2010)
   PUBMED   20348259
REFERENCE   5  (bases 1 to 8833490)
  AUTHORS   Takahashi,S., Ueki,M., Toyoda,A., Ishikawa,J., Sakaki,Y. and
            Osada,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2017) Contact:Shunji Takahashi Chemical Biology
            Research Group, RIKEN Center for Sustainable Resource Science
            (CSRS); Hirosawa 2-1, Wako, Saitama 351-0198, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Phrap v. 0.990329
            Genome Coverage                   :: 16x
            Sequencing Technology             :: ABI 3730
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016756395.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 03:58:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,420
            CDSs (total)                      :: 7,334
            Genes (coding)                    :: 7,181
            CDSs (with protein)               :: 7,181
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 35 of 153
            Pseudo Genes (incomplete)         :: 136 of 153
            Pseudo Genes (internal stop)      :: 8 of 153
            Pseudo Genes (multiple problems)  :: 25 of 153
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:29:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3425893
            Orig. end                         :: 3436811
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP018365.1.
            This work was done in collaboration with Shunji Takahashi, Masashi
            Ueki, Atsushi Toyoda, Yoshiyuki Sakaki and Hiroyuki Osada (RIKEN)
            and Jun Ishikawa (National Institute of Infectious Diseases).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10918
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="18"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10918
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10918
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5918](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5918
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            502..2535
                     /locus_tag="RVR_RS14080"
                     /old_locus_tag="RVR_3967"
     CDS             502..2535
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143181.1"
                     /locus_tag="RVR_RS14080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_3967"
                     /product="AAA family ATPase"
                     /protein_id="WP_237405311.1"
                     /transl_table=11
                     /translation="MGRERAHLAASRAALRAMRQDVEALDISDVTGNWVNAKILAGEIE
                     VRIKALADLAGTPLFFGRLDYLHAPGVVAAEGAGGESFYIGRRHVHDAGGDPMVIDWRA
                     PVSQPFYRASRVEPMDVRLRRRFGYTGGDLTAYEDEHLTDPAEADRSSKLLQSEIERPR
                     VGPMRDIVATIQPEQDEIVRAGVGGTVCVQGAPGTGKTAVGLHRVAYLLYAHRERLART
                     GTLVIGPNASFLQYIEQVLPALGELQVKQATVDDLVAHVPVRGTDTAATARLKGDARMA
                     EVLRRAVRSGISMPTEPVVVVRGSRRWRVPAYELEEIVREMADRDIRYGAAREALPQRI
                     AHAVLVQMERSGEAPDDRVQDAVARNAAVKAAVKACWPPVDPAKTVLRLLSDPEFLASC
                     ADGLLSEQEQQVLLWAKPARGLASAKWSAADAVLVDEANDLVQRTGSLGHVVLDEAQDL
                     SPMQYRAVGRRCSTGSATILGDLAQGTTPWATGSWAEALSHLGKPDARVEELTRGFRVP
                     REVIAYASRLLPSIAPGLAPATSVRESPGALYVRPTAEDALDAAVVAACRESLGHEGSI
                     GLIAADPRVPALAAALDAAGLPYLSPGEETSAAARLTLVPASLAKGLEYDYVVMDDPAA
                     IVAAEPDERTGLRRLYVCLTRAVSGLTVLHCAPLPAALAAAG"
     gene            complement(2632..3342)
                     /locus_tag="RVR_RS14085"
                     /old_locus_tag="RVR_3969"
     CDS             complement(2632..3342)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014061507.1"
                     /locus_tag="RVR_RS14085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_3969"
                     /product="copper homeostasis protein CutC"
                     /protein_id="WP_202234184.1"
                     /transl_table=11
                     /translation="MSRPIFEVIALGAEDAVAAQEGGADRLELVTDMAADGLTPSRATF
                     GAIRSAVRIPLRVMLRLSDGFGAGDARELDVLCETAQGLRAEGADEFVLGFLDARGRAD
                     LDAVHALVDVIDGCRWTFHRAMDRAVDRDALRLQLAGLPGLDTYLTAGSAHGVGAGLTV
                     LCDEAARSGEPGYEPELLVGGGLLLDHVPMLRAAGLAGFHVGGAVRPGGWAAPVDVSAV
                     RTWREALDSPVAVG"
     misc_feature    3305..3320
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.92"
                     /tool="antismash"
     misc_feature    complement(3329..3344)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.49"
                     /tool="antismash"
     misc_feature    3336..3349
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.12"
                     /tool="antismash"
     gene            complement(3428..4096)
                     /locus_tag="RVR_RS14090"
                     /old_locus_tag="RVR_3970"
     CDS             complement(3428..4096)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355127.1"
                     /locus_tag="RVR_RS14090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_3970"
                     /product="hypothetical protein"
                     /protein_id="WP_202234185.1"
                     /transl_table=11
                     /translation="MPESRAEAHPRHAALLAEWSELLRRCRSGADETDPAVYGRELLAR
                     WAEPQRRYHTLDHLVAVLARVTELTVYAHDPDTVALAAWFHDAVYRPDRSENEERSAAL
                     AERALAEAGVDAERIAQVARLVRLTVDHDPAEGDADGEVLCDADLAVLAGDPAAYAAYA
                     AEVREEYGFVPDEDFRAGRAEVLRRLLALPRLFRTEYGRTHWEQVARRNVGTELELLEA
                     "
     misc_feature    4094..4109
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.99"
                     /tool="antismash"
     gene            complement(4187..4828)
                     /locus_tag="RVR_RS14095"
                     /old_locus_tag="RVR_3972"
     CDS             complement(4187..4828)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019062749.1"
                     /locus_tag="RVR_RS14095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_3972"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_202234186.1"
                     /transl_table=11
                     /translation="MQGRAETTRRCLVEAAARLFDEHGYAGTSISDISALSGRTSGAIY
                     FHFGNKEQLALSVVEAHFATWPEMIARVRGSYASALEKLVKLSFEVARAFRDDVVVRAG
                     SRLWTERRAIDTPLPAPFLGWIDTVGSLLGEARAQGELAGGLAPETAAVGVVYAFFGMH
                     TVSDALDGRDQIEDRLYDLWLLLLAGLQARPDATALLARVGAVRAPCLVP"
     misc_feature    complement(4943..4956)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.63"
                     /tool="antismash"
     gene            5001..5918
                     /locus_tag="RVR_RS14100"
                     /old_locus_tag="RVR_3973"
     CDS             5001..5918
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543192.1"
                     /locus_tag="RVR_RS14100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_3973"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_237404732.1"
                     /sec_met_domain="AfsA (E-value: 1.2e-18, bitscore: 65.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGLSWSRTVAREWVHRSSVAEVLLTDVRPDRTGGFEAAACWPRS
                     HPTFPRDGSDLHSPLLVVETLRQLGIYLPLRFLGVPQSARLLIGDLYFALSPGREPRAG
                     HGATEVTCAIAVSRVRRDALGAVTGLRLEVHYACGGHRFARAGGGARFVDPDGYAAVRG
                     RHGTPVPAPWSPPPPAHTHAADDRPSPAALAVGGAGDVLITRRAGTLAVEPADPRHPFF
                     FDHASDHVPGMVLLEAARQAAAEASGGTLLRPLTGRMVARRFTEFTPPALIEPVPHDRT
                     CVFRVRQGPACTAYGVLGYDRHAV"
     misc_feature    5920..5938
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 16.61"
                     /tool="antismash"
     misc_feature    6051..6067
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 18.65"
                     /tool="antismash"
     gene            6140..6808
                     /locus_tag="RVR_RS14105"
                     /old_locus_tag="RVR_3974"
     CDS             6140..6808
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947697.1"
                     /locus_tag="RVR_RS14105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_3974"
                     /product="HAD family phosphatase"
                     /protein_id="WP_202234188.1"
                     /sec_met_domain="HAD_2 (E-value: 9.2e-23, bitscore: 80.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSHPLFSWTPTAIVFDCDGTLMDTERHWQDARDLTLRDFRLTTA
                     DGFAERSKGLHYTECGQLMADESGRPELGGELTERLLDHFRALVAEDPQTMPGAGELVA
                     NAAKFAPLAVASNCPLDVVDTCLRTANLRQHFDHIVVPGPDVRPKPHPDVYLTAVRLCG
                     ADPAESLAVEDSACGIEAATRAGLRTMGVGPWPGEDLAATVDIWVASLDDPNIFSWYSR
                     "
     gene            complement(6878..7624)
                     /locus_tag="RVR_RS14110"
                     /old_locus_tag="RVR_3976"
     CDS             complement(6878..7624)
                     /GO_function="GO:0004371 - glycerone kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151396.1"
                     /locus_tag="RVR_RS14110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_3976"
                     /product="DAK2 domain-containing protein"
                     /protein_id="WP_202234189.1"
                     /transl_table=11
                     /translation="MLTSTDTGFPEFSEPFRSTGFTDFTEGWVRRFAASAAATEAELTA
                     LDQQVGDGDFGANLVAGLQAALRRLGAPGVPASPPAVPASPAVPAPPAWPLEAAASAFL
                     DEVGGTSGPLFGLLLQEIAVSVAAGDGPPAVALATGAGGGLAAIQRVGEAAVGDKTLVD
                     ALAPACDALAAQARTAPGDPSAAAAEGARAAWGGVRSTARFTARRGRASYLGERAAGVP
                     DPGAVGIALLFASAATTVPTLAPYLG"
     gene            complement(7752..8759)
                     /locus_tag="RVR_RS14115"
                     /old_locus_tag="RVR_3977"
     CDS             complement(7752..8759)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058102.1"
                     /locus_tag="RVR_RS14115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_3977"
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="WP_202234190.1"
                     /transl_table=11
                     /translation="MGLYFVNAADELVGDALAGFARAHADRVRWDERDGYVQALKPAPT
                     RRVGLVSGGGSGHEPLHTGYVGTGMLDAACPGRVFASPHNRQIFAASRAVAGDGGVLHV
                     VKNYTGDKINFGIAAERLAREGIACGRVLVDDDVATESDEVATGRRGTGATVLVEKILG
                     AAADTGMGLAELVELGTDVAGRCRSLAVASGAHTSPATGRPAFDLPPGAIEYGVGIHGE
                     RARRTLAERSLRDLAEDMVGSLADSLDLGPRARVVLLVNGLGGVTRLELYAVLGEVARL
                     LDKRGATPVRTLVGDYVTALDMRGFSLTLLAADDATLGYYDAPAAAPAWCGRAG"
     gene            complement(8913..9776)
                     /locus_tag="RVR_RS14120"
                     /old_locus_tag="RVR_3979"
     CDS             complement(8913..9776)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1260: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548609.1"
                     /locus_tag="RVR_RS14120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_3979"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_202234191.1"
                     /transl_table=11
                     /translation="MPTTRQSASRSPERPARHPRDPGPHPAGGPAPRPLDGPTRQELVH
                     RERIDWHHHAVDQLVHPSSGVLRVSTPLGAWVVPPYRAVWIPAGVPHAHEAHGRTDMRT
                     LSFPPGNGPFGDDRPTVVAVPPLLRELVVALTDAPDAAHPPYTAAQRADLERVALVQLR
                     RVEALPLYLPASDDDRLRAVAALLRADPADDRTLAELGAAVGAGERTLSRLFRAELGMT
                     FPQWRTQLRLHHSLTLLAAGHSVTSVATACGYANASAFIEAFRLAFGVTPGKGRTRPPG
                     LPGPPR"
     misc_feature    complement(10165..10180)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.11"
                     /tool="antismash"
     misc_feature    10841..10855
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
ORIGIN
        1 gtggccgggg cgggcgccga gcgcggtgcc gccgtggacg gtgggacctg ggggaagtgt
       61 ggtggaggcc atgtctccac cgtagggatc acacgggcgg ccgacgtcgg gccatggacg
      121 gatatcggga gccgcggctg cgacctcggg cggacccgcg accctgacgg gcgtgtcggg
      181 gtcaccccga gaagggcggc ggccggccgc ccggaaaccg gttcgcgccg ccgcggcccg
      241 gcgcgtatag ttccgcgtct tgccgcctcc ctgacgggag tgccgccgcc cggacggaaa
      301 ccggccggcc ccttcgtgtc accaccgcgt gcgtacgcgc cgccgtacgc gcccacgtac
      361 ctgcccggag gccaagccgt gccctcgcac gcccccgacc agcccgtccc gacgaccccc
      421 gcggcgtcct ccgccgccgg cgccaccgac gctcccgccg acgccggcga cgctcccggc
      481 gccaccgacg ccaccggccc gctgggccgc gagcgcgccc acctcgcggc ctcccgggcc
      541 gccctgcgcg ccatgcggca ggacgtggag gcactcgaca tcagcgacgt caccgggaac
      601 tgggtcaacg cgaagatcct cgccggcgag atcgaggtgc gcatcaaggc gctcgccgac
      661 ctcgccggca ccccgctctt cttcggccgc ctcgactacc tgcacgcacc gggcgtcgtg
      721 gccgccgagg gggcgggcgg ggagagcttc tacatcggcc gccgccacgt gcacgacgcc
      781 ggcggcgacc cgatggtcat cgactggcgt gccccggtct cgcagccgtt ctaccgcgcc
      841 tcccgggtcg agccgatgga cgtgcggctg cgccgccgct tcggctacac cggcggcgac
      901 ctgaccgcgt acgaggacga gcacctgacc gacccggccg aggcggaccg ttccagcaag
      961 ctgctccagt cggagatcga gcggccgcgc gtcgggccga tgcgcgacat cgtggccacg
     1021 atccagcccg agcaggacga gatcgtgcgc gccggggtcg gcggcaccgt ctgcgtgcag
     1081 ggcgcgcccg gcaccgggaa gaccgcggtc ggcctgcacc gggtggccta cctgctctac
     1141 gcgcaccggg agcggctggc ccgcaccggc accctggtga tcggaccgaa cgcgtcgttc
     1201 ctccagtaca tcgagcaggt gctgcccgcg ctgggcgaac tccaggtcaa gcaggccacc
     1261 gtggacgacc tggtggccca cgtcccggtg cgcggcaccg acacggcggc caccgcgcgg
     1321 ctgaagggcg acgcgcgcat ggcggaggtg ctgcggcgcg cggtccgctc gggcatcagc
     1381 atgccgaccg agccggtcgt ggtggtgcgc ggttcacggc gctggcgggt gcccgcctac
     1441 gaactggagg agatcgtccg ggagatggcc gaccgcgaca tccggtacgg ggccgcccgc
     1501 gaggcgctgc cgcagcggat cgcgcacgcg gtcctggtgc agatggagcg gtccggcgag
     1561 gcgcccgacg accgggtgca ggacgcggtg gcacgcaacg cggcggtgaa ggccgcggtc
     1621 aaggcgtgct ggccgccggt cgatccggcc aagaccgtgc tgcggctgct gtcggacccg
     1681 gagttcctcg cctcctgcgc cgacgggctg ctgtcggagc aggagcagca ggtgctgctg
     1741 tgggcgaagc cggcccgcgg gctcgcctcc gcgaagtggt cggcggccga cgcggtgctg
     1801 gtcgacgagg cgaacgacct ggtgcagcgc accggttcgc tcggccacgt ggtgctcgac
     1861 gaggcgcagg acctctcgcc gatgcagtac cgcgcggtgg ggcggcgctg ctccaccggg
     1921 tcggccacga tcctgggcga cctggcgcag ggcaccacgc cgtgggcgac cgggtcctgg
     1981 gccgaggcgc tgtcgcacct gggcaagccg gacgcccgcg tcgaggagct gacccggggg
     2041 ttccgcgtgc cgcgcgaggt catcgcctac gcgtcccggc tgctgccgtc catcgccccc
     2101 ggcctcgcac cggcgacctc cgtccgcgag tcgcccggcg cgctctacgt gcggccgacc
     2161 gccgaggacg cgctcgacgc ggccgtggtc gccgcctgcc gcgagtcgct cggccacgag
     2221 ggctccatcg ggctcatcgc ggccgacccg cgcgtcccgg cgctggcggc cgcgctcgac
     2281 gccgccggcc tgccgtacct gtcccccggc gaggagacct cggcggccgc ccgcctcacc
     2341 ctggtccccg cctcgctggc caagggcctg gagtacgact acgtggtgat ggacgacccc
     2401 gccgcgatcg tcgcggccga acccgacgag cggaccggtc tgcgccgcct gtacgtctgc
     2461 ctcacccgtg cggtctcggg tctgacggtg ctgcactgcg ccccgctgcc ggccgccctg
     2521 gcggcggccg gctgaaccgg ggccgggccg acccggggcc ggccgacccg gggttcgacg
     2581 ggcccggggt tcgacgggcc cggggttcga cgggctcctg gtgacgccgg ctcagccgac
     2641 ggccaccggc gagtcgagcg cttcgcgcca ggtgcgcacg gcggacacgt ccacgggtgc
     2701 ggcccatccg ccgggccgca ccgcgccgcc gacgtggaag ccggccagcc cggcggcgcg
     2761 cagcatgggc acgtggtcga gcagcagccc cccgccgacc agcagttccg gctcgtagcc
     2821 gggctcgccg gaccgtgcgg cctcgtcgca cagcaccgtc agcccggccc cgacgccgtg
     2881 cgcggagccg gcggtcaggt aggtgtccag gccgggcagg ccggcgagtt gcaggcgcag
     2941 cgcgtcacgg tcgacggcgc ggtccatcgc gcggtggaag gtccagcggc agccgtcgat
     3001 cacgtcgacc agcgcgtgca ccgcgtcgag gtcggcgcgg ccgcgcgcgt cgaggaagcc
     3061 gagcacgaac tcgtcggcgc cctcggcccg caggccctgc gcggtctcgc acagcacgtc
     3121 cagctcgcgg gcgtcgccgg cgccgaagcc gtcggacagc cgcagcatga cgcgcagcgg
     3181 gatgcgcacc gcggacctga tcgcgccgaa ggtcgcgcgg gagggtgtga gcccgtcggc
     3241 ggccatatcg gtgaccagtt cgaggcggtc ggcgccgccc tcctgggccg caacggcatc
     3301 ctccgcgccg agggcgatca cctcgaagat tggtctagac atatgagcaa gggtgccaca
     3361 ccagccccat ccgcgcgagg gtcggggggc atcgacggcg ccgcgggggc ccgggtcgga
     3421 cggtggccta tgcctccagc aactccagtt cggtgccgac gttgcggcgg gccacctgct
     3481 cccagtgggt ccggccgtac tcggtccgga acagccgcgg cagcgcgagc agccggcgca
     3541 gcacctcggc gcggccggcc cggaagtcct cgtccggcac gaagccgtac tcctcgcgga
     3601 cctcggcggc gtacgcggcg tacgccgccg ggtcgccggc cagcaccgcc aggtcggcgt
     3661 cgcacagcac ctcgccgtcg gcgtcgccct cggccgggtc gtggtccacc gtcagccgta
     3721 ccagccgggc gacctgtgcg atccgctccg cgtccacccc ggcctcggcc agcgcccgtt
     3781 cggccagcgc ggcgctgcgc tcctcgttct cgctgcggtc cgggcggtag accgcgtcgt
     3841 ggaaccaggc ggcgagcgcc accgtgtccg ggtcgtgcgc gtacaccgtc agttccgtca
     3901 cccgggccag caccgcgacc aggtggtcga gcgtgtggta gcgccgctgc ggctccgccc
     3961 accgggccag caactcccgc ccgtagacgg cgggatcggt ctcgtccgca ccgctccggc
     4021 agcgcctcag cagctcggac cactccgcga gcagggcggc gtgacgggga tgggcttcag
     4081 ctcggctctc gggcatgccg atcattgtcc acggccggcc cgggtgcccg tccccacccc
     4141 ccggggcggc acggcgggcc gaccgcgcgg ctcccgacgg ggccggtcac ggcaccaggc
     4201 agggcgcgcg caccgcgccc acccgcgcca gcagggcggt cgcgtcgggc ctggcctgga
     4261 gcccggcgag cagcagcagc cacaggtcgt agagccggtc ctcgatctgg tcgcgcccgt
     4321 cgagcgcgtc ggagacggtg tgcatcccga agaacgcgta gaccacgccg accgcggccg
     4381 tctcgggcgc gagcccgccc gccagttcac cctgcgcgcg cgcctcgccc agcagcgagc
     4441 ccacggtgtc gatccagccg aggaacggcg cgggcagcgg ggtgtcgatg gcccgccgct
     4501 cggtccacag ccgcgacccg gcgcgcacca ccacgtcgtc gcggaaggcg cgggcgacct
     4561 cgaagctcag cttcaccagc ttctccagcg cggacgcgta cgaaccccgg acccgggcga
     4621 tcatctccgg ccaggtcgcg aagtgcgcct cgacgaccga gagggccagt tgctccttgt
     4681 tgccgaaatg gaagtagatc gcaccgctgg tacgcccgga cagggcgctg atatcgctga
     4741 tactggtgcc ggcataaccg tgttcgtcga acaaccgcgc cgccgcctcc accagacacc
     4801 tgcgcgtcgt ctccgctcgc ccctgcacat tcactccgat agccgcttcg tcgccgacga
     4861 cggcaatcta gcgtgtcgcg cacaccgaac ggcacctggc ccctgatgac gcgcgtgcgt
     4921 atctgccgga cgtgagttct tcttatggta tgaattcttt ggtcggggat gactccggac
     4981 ggaactccgg aaatggcgca atgccgggcc tgagctggtc ccgcacggtc gcgcgggaat
     5041 gggtgcaccg ctcgtcggtg gccgaggtac tgctcaccga cgtacggccg gaccgaacgg
     5101 gggggttcga ggcggcggcg tgctggccgc gctcgcaccc gaccttcccc cgcgacgggt
     5161 cggacctgca cagcccgctg ctggtggtcg agacactgcg ccaactcggc atctacctgc
     5221 cgctgcgctt cctcggggtg ccgcagagcg cccgtctgct catcggcgac ctgtacttcg
     5281 cgctctcccc cgggcgggag ccgcgggccg gccacggcgc caccgaggtg acctgcgcga
     5341 tcgcggtcag ccgggtccgc agggacgccc tcggcgcggt caccggtctg cggctggagg
     5401 tgcactacgc ctgcggcggc caccggttcg cgcgggccgg cggcggcgcc cgcttcgtcg
     5461 acccggacgg ctacgcggcg gtgcggggcc ggcacggcac ccccgtgccg gcgccctggt
     5521 caccgccccc gcccgcgcac acgcacgccg cggacgaccg cccctccccc gccgcgctcg
     5581 cggtcggcgg cgccggagac gtgctgatca cccggcgcgc gggcaccctc gcggtcgaac
     5641 cggccgaccc gcgccacccg ttcttcttcg accacgccag cgaccacgtg cccgggatgg
     5701 tgctgctgga ggcggcccgg caggccgcgg ccgaggcgag cggcggcacg ctgctgcggc
     5761 cgctcaccgg ccgcatggtg gccaggcgct tcaccgagtt cacgccgccg gccctgatcg
     5821 aaccggtgcc gcacgaccgc acatgtgtct tccgcgtccg gcagggcccg gcctgcaccg
     5881 cctacggagt cctcggatac gaccgacacg cggtgtgact tattgcattc acttgactac
     5941 catgcgcggc cgtccataac gtaacgatcg atatgttctc ggcggtcccc tcctaattcc
     6001 gcgcagtccg cgggatttac ggacgcgctc gccgagagcc cgcgcccgcc tgacgaaaca
     6061 gcgcgcatcc gtctgtcggc ctgcattacc gacggccctc tcggcgcttt caggcattcc
     6121 tcgaacggac cttcccccca tgacctctca ccccctcttt tcgtggaccc cgacggcgat
     6181 cgtcttcgac tgcgacggca ccctgatgga caccgagcgg cactggcagg acgcccgcga
     6241 tctgaccctg cgtgatttcc ggctgaccac ggcggacggt ttcgccgagc ggagcaaggg
     6301 cctgcactac acggaatgcg gtcagctgat ggccgacgaa tcggggcgcc cggaactcgg
     6361 cggcgaactc accgagcggc tgctcgacca tttccgcgcg ctcgtcgcgg aggacccgca
     6421 gaccatgccc ggcgcgggcg aactcgtcgc caacgcggcg aagttcgccc cgctcgcggt
     6481 cgccagcaac tgcccgctcg acgtcgtcga cacctgcctg cgcaccgcca acctccggca
     6541 gcacttcgac cacatcgtgg tgcccgggcc cgacgtccgc cccaagccgc accccgacgt
     6601 ctacctcacc gcggtacgcc tgtgcggcgc cgaccccgcg gagtccctcg ccgtggagga
     6661 ctccgcctgc ggcatcgagg ccgccacccg cgcgggcctg cgcaccatgg gcgtcggccc
     6721 ctggcccggc gaggacctcg ccgccaccgt cgacatctgg gtggcctcgc tcgacgaccc
     6781 gaacatcttc tcctggtact cccgctgacc ggcgggacga agccgggcgg gcggtcccga
     6841 tcccagcggg accgtccgcc caccccctcc tcgcacgtca gcccaggtac ggcgccaggg
     6901 tgggcacggt ggtggccgcc gaggcgaaga gcagggcgat cccgaccgct cccgggtcgg
     6961 gcacgcccgc cgcccgctcg ccgaggtagc tcgcccgccc gcgccgcgcc gtgaaccggg
     7021 cggtgctgcg caccccgccc cacgcggccc gcgccccctc ggcggccgcg gcggacgggt
     7081 cgcccggcgc cgtgcgggcc tgcgcggcga gcgcgtcgca ggccggcgcc agcgcgtcga
     7141 ccagggtctt gtccccgacc gcggcctccc cgacccgctg gatcgcggcg agcccgccgc
     7201 cggcgcccgt cgccagcgcg acggcgggcg gaccgtcccc ggcggccacg gagaccgcga
     7261 tctcctggag cagcaacccg aacagcgggc cgctggtgcc gccgacctcg tcgaggaacg
     7321 cggaggcggc ggcctccagc ggccacgcgg gcggggccgg cacggcgggc gaggcgggta
     7381 cggccggggg tgacgccggc acgccgggcg cgcccagccg ccgcagcgcg gcctgcaacc
     7441 cggccaccag gttcgccccg aagtcgccgt cgcccacctg ctggtccagg gccgtcagtt
     7501 cggcctcggt cgcggcggcg gaggcggcga acctgcgcac ccagccctcg gtgaagtcgg
     7561 tgaagccggt ggagcggaag ggctcggaga actcggggaa cccggtgtcg gttgatgtga
     7621 gcatcgctgc gtctcccgtc gttgcctgcc cggcggtcgt accgggcccc gtgggacgcc
     7681 cggcggtggg cacctggtca tggcgcctgc tcccgggcac cctcggcgcg ggcgcccggt
     7741 cgcgggcgcg gtcagcccgc ccgcccgcac cacgcggggg cggcggccgg ggcgtcgtag
     7801 tagccgagcg tcgcgtcgtc ggcggcgagc agcgtgagcg agaagccccg catgtccagc
     7861 gcggtcacat agtcgccgac gagggtccgt acgggcgtgg cgccgcgctt gtccagcagc
     7921 cgcgccacct cgcccagtac cgcgtacagc tccagacggg tgactccgcc cagaccgttg
     7981 acgaggagca cgacccgcgc ccgcgggccc aggtccaggg agtcggccag ggagccgacc
     8041 atgtcctcgg cgaggtcgcg cagcgagcgc tccgccagcg tgcggcgggc gcgctcgccg
     8101 tggatgccga cgccgtactc gatggcgccg ggcggaaggt cgaacgcggg gcggccggtg
     8161 gcgggcgagg tgtgcgcgcc cgaggcgacc gcgaggctgc ggcaccggcc ggccacgtcc
     8221 gtgcccagct cgaccagctc cgccaggccc atcccggtgt ccgcggccgc gccgaggatc
     8281 ttctcgacca ggacggtcgc gccggtgccg cgccgtccgg tggcgacctc gtccgactcc
     8341 gtcgccacgt cgtcgtcgac cagcacccgg ccgcaggcga tgccctcccg ggcgaggcgt
     8401 tccgcggcga taccgaagtt gatcttgtcg ccggtgtagt tcttcaccac gtgcagcacg
     8461 ccgccgtcac ccgccacggc gcgcgacgcg gcgaagatct gccggttgtg gggcgaggcg
     8521 aagacacggc ccgggcaggc ggcgtccagc atcccggtcc cgacgtagcc ggtgtgcagc
     8581 ggttcgtggc ccgaaccgcc accggagacg aggccgaccc ggcgggtcgg cgccggtttc
     8641 agcgcctgta cgtagccgtc ccgctcgtcc cagcgcacgc ggtccgcgtg ggcccgggcg
     8701 aagccggcca gtgcgtcccc caccagttcg tcggctgcgt tcacgaagta aaggcccatg
     8761 agacgacctc ctgtcacctc atgctgagcg gtcccgggga gcgcggcaac cggacctgcg
     8821 ggtgcccttg tcccgcccgg cccgcgaccg cctcccggtg gagcgtcccg gtgggggcgt
     8881 cccgccgggt ccccccgtcc ggcgggtgcc cctcaacggg ggggaccggg gaggccgggg
     8941 ggacgcgtgc ggcccttccc gggggtgacc ccgaaggcga ggcggaacgc ctcgatgaag
     9001 gcactggcgt tggcgtaccc gcaggcggtg gcaacggagg tgacggagtg gcccgccgcg
     9061 agcagcgtca gcgagtggtg gagccgcaac tgggtccgcc actgcgggaa cgtcatcccc
     9121 agctccgccc ggaacagccg gctcagcgtc cgctcccccg cccccaccgc cgcccccagc
     9181 tccgccagcg tccggtcgtc cgcgggatcg gcgcgcagga gcgcggcgac ggcccgcagc
     9241 cggtcgtcgt cggacgccgg gaggtacagc gggagcgcct ccacccggcg aagctggacg
     9301 agcgcgacgc gttcgaggtc ggcgcgctgg gccgcggtgt acggcgggtg tgcggcgtcg
     9361 ggcgcgtccg tgagcgcgac caccagctcc cgcagcagtg gcgggaccgc caccacggtg
     9421 ggccggtcgt cgccgaacgg cccgttcccg ggcgggaacg acagggtgcg catgtcggtg
     9481 cgcccgtgcg cctcgtgcgc gtgcggcacc cccgcgggaa tccacaccgc gcggtagggc
     9541 ggcaccaccc acgcccccag tggcgtggac acccgcagca ccccgctgct cgggtggacg
     9601 agctggtcca cggcgtggtg gtgccagtcg atgcgctcgc ggtgcaccag ttcctggcgg
     9661 gtgggcccgt ccagcgggcg gggcgcgggg ccgcccgcgg ggtgcggtcc gggatcacgg
     9721 gggtgccggg cgggccgttc gggggaccgg gaggccgatt ggcgggttgt cggcataaac
     9781 agacagagta tcggtagccg accatccgac ccgctcggca ggcttccggt atgacccgga
     9841 ccaccgacac ggcagcctcc cgaccggaca gcgaacgacc ggacacctcc cgaccggacc
     9901 cctcccgccc cccgaccccg acccgcggca cctggcggcg gatgcgcctg tggggcgtcg
     9961 cccacgccgt ggacgacctc taccagggcc tggtccccgc gaccgtgccg tacttcgtcc
    10021 tcgaccggca ctacgggtac gtcgccgcat ccggcctcac cctcgccgcc acgctcggca
    10081 gttcggtgcc gcagccgttc atcggactgg cggtggaccg gatgcggctg ggctggctcg
    10141 cgggggcggg ggtgggcctc gccgggatcg ccctcggcct gtccggcgtc gtgccctcgt
    10201 acgccgccgt gtggtgcctg atcatgctgt ccgggctcgg catcgcgatg ttccacccgg
    10261 ccgcggggaa gtccgcgcgc gaggacgccg gcgacagcgc cgccgcgatg agcgtcttcg
    10321 cggccggcgg cagcgtcggc ttcttcctcg cgccggtcct cgccacgccc gcgctgatcg
    10381 cctggggcgt cggcgccacc gccctgttca tcccgcccgc cgtcctcacc gcgtacgtcc
    10441 tgctgcgcca ccgccgccgg caggcgccga gcgcgtcggc ggcggccgcg ggcggggtgg
    10501 accggtgggg cccgttcgcc gcgctgaccg gcgtcgaagt ggtgcgctcc gtggccttct
    10561 tcggggtgaa caccttcatc gagctgtact ggatcaggca cctgcacgcc tcccggggac
    10621 tggccggggt cgcgctcacc ggcttcctcg ccggcggggt cggggggacc ctgctgggcg
    10681 gccggatcgc ggaccggatc ggcatggtcc gcacggtgca gctcggcggc gcgctcgcgc
    10741 tgcccatgct cgccgggctg ctgctcaccc cgggccgcgt gacacccctg ctgttcgcgg
    10801 tcctcaccgg ggtctgcctc aacctgccct tcgcggtgct ggtcaagctc ggccaggact
    10861 acctgcccac ccgcccgggc accgcggccg gggtcaccct ggggctcggc gtgagcgt
//
