LOCUS       NZ_AP018365            10819 bp    DNA     linear   CON 21-MAY-2024
DEFINITION  Streptomyces sp. SN-593 chromosome.
ACCESSION   NZ_AP018365
VERSION     NZ_AP018365.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. SN-593
  ORGANISM  Streptomyces sp. SN-593
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Panthee,S., Kito,N., Hayashi,T., Shimizu,T., Ishikawa,J.,
            Hamamoto,H., Osada,H. and Takahashi,S.
  TITLE     beta-carboline chemical signals induce reveromycin production
            through a LuxR family regulator in Streptomyces sp. SN-593
  JOURNAL   Sci Rep 10 (1), 10230 (2020)
   PUBMED   32576869
  REMARK    DOI:10.1038/s41598-020-66974-y Publication Status: Online-Only
REFERENCE   2
  AUTHORS   Takahashi,S., Toyoda,A., Sekiyama,Y., Takagi,H., Nogawa,T.,
            Uramoto,M., Suzuki,R., Koshino,H., Kumano,T., Panthee,S., Dairi,T.,
            Ishikawa,J., Ikeda,H., Sakaki,Y. and Osada,H.
  TITLE     Reveromycin A biosynthesis uses RevG and RevJ for stereospecific
            spiroacetal formation
  JOURNAL   Nat Chem Biol 7 (7), 461-468 (2011)
   PUBMED   21642985
  REMARK    Publication Status: Online-Only
REFERENCE   3
  AUTHORS   Panthee,S., Takahashi,S., Takagi,H., Nogawa,T., Oowada,E.,
            Uramoto,M. and Osada,H.
  TITLE     Furaquinocins I and J: novel polyketide isoprenoid hybrid compounds
            from Streptomyces reveromyceticus SN-593
  JOURNAL   J. Antibiot. 64, 509-513 (2011)
REFERENCE   4
  AUTHORS   Takahashi,S., Takagi,H., Toyoda,A., Uramoto,M., Nogawa,T., Ueki,M.,
            Sakaki,Y. and Osada,H.
  TITLE     Biochemical characterization of a novel indole prenyltransferase
            from Streptomyces sp. SN-593
  JOURNAL   J Bacteriol 192 (11), 2839-2851 (2010)
   PUBMED   20348259
REFERENCE   5  (bases 1 to 8833490)
  AUTHORS   Takahashi,S., Ueki,M., Toyoda,A., Ishikawa,J., Sakaki,Y. and
            Osada,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2017) Contact:Shunji Takahashi Chemical Biology
            Research Group, RIKEN Center for Sustainable Resource Science
            (CSRS); Hirosawa 2-1, Wako, Saitama 351-0198, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Phrap v. 0.990329
            Genome Coverage                   :: 16x
            Sequencing Technology             :: ABI 3730
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016756395.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 03:58:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,420
            CDSs (total)                      :: 7,334
            Genes (coding)                    :: 7,181
            CDSs (with protein)               :: 7,181
            Genes (RNA)                       :: 86
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 35 of 153
            Pseudo Genes (incomplete)         :: 136 of 153
            Pseudo Genes (internal stop)      :: 8 of 153
            Pseudo Genes (multiple problems)  :: 25 of 153
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:29:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 651403
            Orig. end                         :: 662222
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP018365.1.
            This work was done in collaboration with Shunji Takahashi, Masashi
            Ueki, Atsushi Toyoda, Yoshiyuki Sakaki and Hiroyuki Osada (RIKEN)
            and Jun Ishikawa (National Institute of Infectious Diseases).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10819
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10819
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10819
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5819](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5819
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(92..107)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.44"
                     /tool="antismash"
     misc_feature    352..365
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.29"
                     /tool="antismash"
     misc_feature    complement(775..789)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.02"
                     /tool="antismash"
     gene            1856..2344
                     /locus_tag="RVR_RS02770"
                     /old_locus_tag="RVR_774"
     CDS             1856..2344
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829856.1"
                     /locus_tag="RVR_RS02770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_774"
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="WP_202232254.1"
                     /transl_table=11
                     /translation="MGLSAHVRKTPLPGVGSRYDLDTDAGGHLSVVVHQDGRRELALRD
                     PQDDDACAGALPLTEAEAGALARLLLPDPVAKLRRAQVGIDLVTERIEIDSRSPYAGRT
                     LGATQARTRTGASIVAVLRRTSAHPSPTPDFRFALGDTLVVVGTREGVDAVAELITGG"
     gene            2351..3724
                     /locus_tag="RVR_RS02775"
                     /old_locus_tag="RVR_775"
     CDS             2351..3724
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /GO_process="GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1117:
                     sodium/hydrogen exchanger"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557693.1"
                     /locus_tag="RVR_RS02775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_775"
                     /product="cation:proton antiporter"
                     /protein_id="WP_202232255.1"
                     /transl_table=11
                     /translation="MHNTTSMLVELGAIILALGLLGRFAGRVGFSPIPLYLLAGLAFGQ
                     GGILPLDASEDFVATGAEIGVVLLLLLLGLEYSASELVTSLRTQYPSGAVDFVLNAVPG
                     AAAALLLGWGPVAAVALAGVTWISSSGVIAKVMRDLRRLGNRETPVILGVLVIEDLAMA
                     LYLPILTALLAGAGLAGGTLTLLISLGTVGAVLYLALRHGRLISRAVSSDNPEMLLLVV
                     LGLTLLVAGVAERLQVSAAVGAFLVGIALSGDVAEGASNLLTPLRDLFAAVFFVFFGLS
                     TVPADIPPVIVPALVLALVTTATKIGTGWFAARRAGIKPPGRWRAGGTLVARGEFSIVI
                     AGLAVATEPRIGPLATAYVLILVVLGPVSARFTEPLARRVTARLPDRAAPVGQPAPAGA
                     TAGAGAGGDGRGDGGDDPFDVRAEAAGDREPDDGTAPDLGADDGTAPGLGPEAAPAKG"
     misc_feature    complement(2355..2369)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.23"
                     /tool="antismash"
     gene            3934..5004
                     /locus_tag="RVR_RS02780"
                     /old_locus_tag="RVR_777"
     CDS             3934..5004
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135065.1"
                     /locus_tag="RVR_RS02780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_777"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_237404531.1"
                     /transl_table=11
                     /translation="MATEPEPQSVLSPLTSAAIFLVLTVEDGGEQTARELLADLPGLTR
                     AIGFGAPDGRLSCVAGVGSAAWDRLFAFPRPADLHPFTPLEGTRHRAPATPGDLLLHLR
                     ATRPDLCFALATEIMKRLRGAVAVQDEVQGFKYLDVRDLLGFVDGTENPVGPAARQAVL
                     VGAEDPEHAGGSYVIVQKYLHDLDAWNALPVEAQERIIGRTKATNIELDADDSHVALNT
                     VTGPDGEEQDILRDNMPFGSPGRGEFGTYFIGYARTPDVTEQMLRNMFLGTSAASHDRI
                     LDFSTAVTGTLFYVPSADFLDDLPDAPARGSAVGTAPPPAAGSGPVPAQADAPGPARPA
                     SDGSLGIGNLNRSTSS"
     gene            5001..5819
                     /locus_tag="RVR_RS02785"
                     /old_locus_tag="RVR_778"
     CDS             5001..5819
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172774.1"
                     /locus_tag="RVR_RS02785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_778"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_202232257.1"
                     /sec_met_domain="Linocin_M18 (E-value: 3.5e-94, bitscore:
                     313.4, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVSDAAWADLEAEARRTFARHVAARRIVDVPEPAGP
                     ELSAVATGHLDRIDAPAQGVTARTRRSQPVVELRVPFTVDRGQVDDVERGAKDADWQPV
                     KDAAKQLAYAEDRAVFEGYAAAGITGIRAASSNPELTLPADVRDYPDAISRAVTTLRLA
                     GVDGAYTLALSADAYTAVSETSDHGYPIHQHLARLLDGDIVWAPAIDGAFLLATRGGDF
                     ELRLGQDVSIGYLGHTATTVDLYFQETLTFLVHTAEAGVALKPSAGPTAV"
     misc_feature    6053..6067
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.07"
                     /tool="antismash"
     gene            6098..6937
                     /locus_tag="RVR_RS02790"
                     /old_locus_tag="RVR_779"
     CDS             6098..6937
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1292: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386535.1"
                     /locus_tag="RVR_RS02790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_779"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_272933053.1"
                     /transl_table=11
                     /translation="MAEEEATFAEETGVSDRLRQDLPHSARMYDYYLGGKTNYAVDRTA
                     AEDVIRRFPSIRTVAQINRAFVHRSARFLARERGILQFIDIGTGIPTQPNLHEVVQREV
                     PQARIVYADNDPIVLVYADNLLSSSPQGATEYVEADVTDPQKLLAAVEAAGCLDFTQPV
                     GLSLNAVLHFVTDAMDAYGVVRTLVDRLAPGSYLAISHCTPDFDPGAWAAITDIYTEGG
                     TPLQVRSKAEVERFFEGLELVEPGVEVAHRWRPEAGEGPTLVTDAQSSLYAGVARKA"
     misc_feature    6149..6151
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7048..7875)
                     /locus_tag="RVR_RS02795"
                     /old_locus_tag="RVR_780"
     CDS             complement(7048..7875)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040183.3"
                     /locus_tag="RVR_RS02795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_780"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_202232259.1"
                     /transl_table=11
                     /translation="MIFGETLRQRRQALGLTAEDVVRQIGHSASKISRMENGRHDFKEA
                     DLRRLLDVYGVHDQGERDLLLDLARAANERGWWDAWSDVSTKTLQTLVSLEDMAQRIRS
                     YEIAQLPGLVQTAEYTRALIRANAPGKSEQEIERIIELRAMRQQRLMREPGKKLLCVLD
                     EVTLVRPYGSREGMRRQLEHLISLADDPDIGFNLVELTKPNLPVQIGTTTIFDFADGQL
                     PDIVYIERSGGGLYLQEEHEVDEHVKGFDRLMIASLGQRATVRRIQDHIKKFC"
     misc_feature    complement(7854..7874)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.13"
                     /tool="antismash"
     gene            complement(8058..8315)
                     /locus_tag="RVR_RS02800"
                     /old_locus_tag="RVR_781"
     CDS             complement(8058..8315)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569512.1"
                     /locus_tag="RVR_RS02800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_781"
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_202232260.1"
                     /transl_table=11
                     /translation="MTGQGEGAQRHVAPRFRPDVAGTAVSWRKSSYSNHESNCVEVAEL
                     GDGTIGIRDSKDRNGPILTVDRTAMRAFVTGVVRGEFAVE"
     gene            complement(8471..8995)
                     /locus_tag="RVR_RS02805"
                     /old_locus_tag="RVR_782"
     CDS             complement(8471..8995)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1198: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /locus_tag="RVR_RS02805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_782"
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="WP_202232261.1"
                     /transl_table=11
                     /translation="MATSARDGDGGRGDRPGASPDASPDAEGAPGAKGSPDPSADASPF
                     PPVEPEALAELALDLPETTEENPFGPGADVYKVGGRIFAILSPDADPPSVSLKCDPALA
                     LHLRDRYAAVTPGYHLNKKHWNTVALDGSIPPDEIDDLLAHSYACVVAGLPKATRDRLA
                     LLTPAVTPPAP"
     gene            complement(9181..9897)
                     /locus_tag="RVR_RS02810"
                     /old_locus_tag="RVR_783"
     CDS             complement(9181..9897)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006350260.1"
                     /locus_tag="RVR_RS02810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RVR_783"
                     /product="beta-glucanase"
                     /protein_id="WP_237404532.1"
                     /transl_table=11
                     /translation="MTVPQSAARPAPVVFAAPFTDRKAWAVGRSSAYPPDGRNPGDNKL
                     DQIGPSYGPTAAGVFRARAAGGGLWHTDLVSTEYAPGGFELLPGDELSATCVVDDTPGA
                     WPALWTWGRDGSSGRPQPGHGEVDAFEYHPTHPHMLELSNHVGGAGDNVDGLVTPGRPF
                     RLDVLLGARSVRWQVDGRTVYDDGHGVGTRWRAWLVVCISVSAGAYGHSAPGPGVRELS
                     WQCTDLEVRRPGAGQG"
ORIGIN
        1 tccgggccgg gggccgaccg ggacgagcgg gatcagcagg acgaccggct gcgcgccggc
       61 acccgcgcct accgccgggc caacctcgcc ctgttcgccg ccggcctggc cacgttcgcg
      121 ctgctgtact ccacccaggc cctgctgccc gcgctctccg cggggctgcg gctgtccccc
      181 gcgcaggcga gtctgacggt gtcggcgagt accggggcgc tcgcgctcac cctgctgccg
      241 gcgagcgcgc tcagcgagaa gtacggacgc acgcgggtga tgacggtctc ggtgttcgcc
      301 gcgtccctgc tggcgctcgc ggtccccttc gccccggacc tcgccgtgct ggtcaccctg
      361 cgcgcgctcc agggcgtcgc gctcgccggg ctgcccgcga ccgcgatggc gtacctcgcc
      421 gaggaggtgc acccctcggc ggtgccctcg gcgatcgggc tgtacgtcgc ggggaactcc
      481 atcggcggga tgagcagccg ggtcgcgggc ggcgcgctcg cccaggtgta cggatggcgt
      541 accgccctgc tcgtggtcgg cgccacggcg ctcgcctgtg ccgtcgcctt ccgcgtcctc
      601 gccccgcccg cgcggcactt cacccccggg tcgatctcgc cggccgccct gcgccgcacc
      661 gtcgccggcc acctccgcga cggccggctg cgccgcctct acgcggtggg gatgctcttc
      721 atggccgtgt tcggagcggt ctacacggtg ctggggtacc ggctcacctc cgcgccctac
      781 acgttgtcgc agtccgcggt cggcgcggtg ttcctggtgt acctcgtcgg cacgggcacc
      841 tccgccgcgt ccggagcgct caccgcgcgc gtcggccggc gcggggcctt cgccgtcgcc
      901 ctcgccctgt gcgcgggcgg gctggcggtg acgctggcgc ggccgctgtg ggcggtgctg
      961 ctcgggctgg tgctcatcac cggcgggttc ttcatcgggc acgcggtcgc gtcgggcgcg
     1021 gtgagccgta cggcgcgcag cggcagggcg caggcgtcgg cgctctacct gacggcgtat
     1081 tacgtcggga acagcctcgg cgggacggtc gcggcgtcgg cctaccacct gtccgggtgg
     1141 gccgcggcgg ccgcggtcgc cttcctcgcg ctgctcctgg ccgcccccgc ggccctgcgc
     1201 cgcgcctgac cgcccccgcg ggccccgcgg agtaaggccc ccgagccgcc cggtcggccg
     1261 gccgacgcgc gcggggccgc cgcgacccgc ctccggtgcg cgggcgcgga cccaccgcgt
     1321 ccacgcgccc ccaggaccac ccgcgcggcc tcccccgccc ccctcggcga gcccgggctg
     1381 tcggccgccg acccgcccgg tacgggagcc gcgcgggggc caccggccgc cgggagcacc
     1441 gaaggccggg tgcaattggg cgcttatacg gcacctcttc cgcattgacc cattacttcg
     1501 tgcggtaggg ttctttacct gacgcatacc aacggcggga gagcgggagg gacggcggga
     1561 tggtgcggca cggaaggacc accgcgctcc cggcagccgt tccgcgggcc gggacgtccg
     1621 gcggcgcccg ggcgacaccc gccgggtgcg caccgcgcga ccgcgttccc gataccggcg
     1681 cctgaggtcc gcacggcccg gccggggggc cgggcgcccc gaccgacggc ccggcacggc
     1741 ccgggaccgt agtgcccgcg ccgccgcggc cgccgacggg accgctcacc gccgcccgcg
     1801 gcacccaccg cgcaggattc cgtcccccac ccccgtacgc cccttggagc acgccatggg
     1861 cctgtcggct cacgttcgca agaccccgct gcccggcgtc ggcagccgtt acgacctcga
     1921 caccgacgcc ggcgggcacc tgtcggtcgt cgtccaccag gacgggcggc gtgaactggc
     1981 gctgcgcgac ccgcaggacg acgacgcgtg cgcgggcgcc cttccgctga ccgaggccga
     2041 ggccggggcg ctggcccggc tgctgctgcc ggacccggtg gcgaagctgc gccgcgcgca
     2101 ggtcggcatc gacctggtca ccgagcggat cgagatcgac agccggtcgc cgtacgccgg
     2161 ccgcacgctc ggcgccaccc aggcccgtac ccgtaccggc gcctcgatcg tcgcggtgct
     2221 gcggcggacc tcggcgcacc cctcgccgac gccggacttc cgcttcgcgc tcggggacac
     2281 gctcgtcgtc gtcggcaccc gcgagggcgt ggacgccgtc gccgaactga tcaccggagg
     2341 atagccgctc gtgcacaaca cgacgtccat gctcgtcgaa ctcggggcga tcatcctcgc
     2401 tctgggcctg ctcggccggt tcgccggccg ggtgggcttc tccccgatcc ccctctacct
     2461 gctcgccgga ctcgccttcg gccagggcgg gatcctcccg ctggacgcga gcgaggactt
     2521 cgtggccacc ggtgccgaga tcggcgtggt cctgctgctg ctcctgctcg gcctggagta
     2581 cagcgcgtcg gaactggtca ccagtcttcg gacgcagtac ccctccggcg cggtggactt
     2641 cgtgctcaac gcggtgcccg gtgccgccgc cgcgctgctg ctgggctggg gcccggtcgc
     2701 cgcggtcgcg ctggccggcg tcacctggat ctcctcctcg ggggtgatcg ccaaggtgat
     2761 gcgcgacctg cgccggctcg gcaaccggga gacgccggtc atcctgggcg tgctggtgat
     2821 cgaggacctg gcgatggcgc tgtacctgcc gatcctcacc gcgctgctgg ccggcgcggg
     2881 gctggccggc ggcacattga ccctgctgat ctcgctgggc acggtcggcg cggtgctcta
     2941 cctggcgctg cgccacggcc ggctgatcag ccgggcggtc tcctcggaca acccggagat
     3001 gctgctgctc gtcgtgctcg gcctgaccct gctggtcgcg ggcgtcgccg aacgcctcca
     3061 ggtctcggcg gccgtcggcg cgttcctggt cggcatcgcg ctgtcgggcg acgtcgccga
     3121 gggcgcgagc aacctcctca ccccgctgcg cgacctgttc gccgcggtgt tcttcgtctt
     3181 cttcgggctc tccacggtcc ccgcggacat cccgccggtg atcgtgcccg cgctggtcct
     3241 cgcgctcgtc accacggcga ccaagatcgg caccggctgg ttcgcggccc gccgcgccgg
     3301 catcaagccg cccggacggt ggcgggcggg cggaaccctg gtggcccggg gcgagttctc
     3361 gatcgtgatc gccgggctcg cggtggccac cgaaccgcgg atcggcccgc tggccaccgc
     3421 ctacgtgctg atcctggtcg tcctcgggcc ggtcagcgcc cgcttcaccg agcccctggc
     3481 gcgccgtgtc accgcccgcc tgccggaccg cgccgcaccg gtcgggcaac ccgccccggc
     3541 cggcgcgacc gcgggcgcgg gcgcgggcgg cgacggccgg ggcgacggag gggacgaccc
     3601 gttcgacgtc cgcgccgaag ccgccgggga ccgcgagccc gacgacggga ccgcgcccga
     3661 cctcggcgcg gacgacggga ccgcccccgg cctcggcccg gaggccgcac ccgccaaggg
     3721 ctgacccgct tccggcaccc ctggacaccc gccggacccg caccggcaca ggcaccggca
     3781 gcggcacccg ggagaacggg tgcggggctc gcctggcgcg gtccggcggc gggcggcggt
     3841 gggccgttcg accgcgccgg aagcccgggc agtagggtcc agatgacgcg ccggccgccg
     3901 cccgcgcggt gcagagcgat cgggagatct ggattggcca ccgagccgga accgcagtcc
     3961 gtactgagcc cgttgacctc cgccgcgatc ttcctggtgc tgacggtcga ggacggcggt
     4021 gagcagaccg cgcgcgagct gctcgccgat ctccccgggc tgacccgcgc catcgggttc
     4081 ggcgcgcccg acgggcggtt gagctgtgtc gccggcgtcg ggtcggccgc gtgggaccgc
     4141 ctgttcgcct tcccgcggcc ggcggacctg caccccttca ccccgctgga gggcacgcgg
     4201 caccgcgcgc cggccacccc cggcgacctg ctcctgcacc tgcgggcgac ccggcccgac
     4261 ctgtgcttcg cgctggccac cgagatcatg aagcggctgc gcggagcggt ggccgtgcag
     4321 gacgaggtgc agggcttcaa gtacctcgac gtccgcgacc tgctgggttt cgtggacggc
     4381 accgagaacc cggtcggccc ggcggcgcgg caggcggtgc tggtcggcgc ggaggacccc
     4441 gagcacgcgg gcggcagcta cgtgatcgtg cagaagtacc tgcacgacct ggacgcctgg
     4501 aacgcgctcc cggtcgaggc gcaggagcgg atcatcgggc gcacgaaggc gaccaacatc
     4561 gagctggacg ccgacgactc gcacgtcgcg ctgaacaccg tcaccggccc ggacggcgag
     4621 gagcaggaca tcctgcgcga caacatgccg ttcggcagcc cgggccgcgg cgagttcggc
     4681 acgtacttca tcggctacgc gcgtaccccg gacgtcaccg agcagatgct gcggaacatg
     4741 ttcctgggca cctcggcggc ctcccacgac cgcatcctcg acttctccac cgccgtcacg
     4801 ggcacgctgt tctacgtgcc ctcggcggac ttcctcgacg acctgccgga cgctccggcg
     4861 cgcgggtccg cggtcggcac ggcgccgccg ccggcggccg ggtccggccc ggtgcccgcg
     4921 caggcggacg cacccggacc cgcgcggccc gcctccgacg gcagcctcgg catcggcaac
     4981 ctgaacagga gcacgtcctc gtgaacaacc tgcaccgcga actcgccccc gtctccgacg
     5041 ccgcgtgggc cgacctggag gccgaggccc ggcgcacctt cgcccgccac gtcgcggccc
     5101 gccgcatcgt ggacgtgccc gagcccgccg ggccggagct gtccgcggtg gcgaccggac
     5161 acctggaccg gatcgacgcg cccgcgcagg gcgtgaccgc ccgcacccgc cgctcgcagc
     5221 cggtggtgga gctgcgggtg ccgttcaccg tggaccgcgg ccaggtcgac gacgtcgagc
     5281 gcggcgccaa ggacgcggac tggcagccgg tcaaggacgc ggccaagcag ctcgcgtacg
     5341 ccgaggaccg ggcggtcttc gaggggtacg ccgccgcggg catcaccggc atccgcgccg
     5401 cgtcgtccaa ccccgagctg acgctccccg cggacgtccg cgactacccg gacgcgatca
     5461 gccgcgcggt caccacgctg cggctggcgg gcgtcgacgg ggcgtacacg ctggcgctga
     5521 gcgcggacgc gtacaccgcg gtcagcgaga cctccgacca cggctacccg atccaccagc
     5581 acctcgcgcg gctgctggac ggcgacatcg tgtgggcgcc ggcgatcgac ggggcgttcc
     5641 tgctcgccac ccgcggcggg gacttcgagc tgcggctcgg ccaggacgtg tcgatcggct
     5701 acctcggcca caccgcgacc acggtggacc tgtacttcca ggagaccctg acgttcctgg
     5761 tgcacaccgc ggaggcgggt gtcgccctca agccgtccgc cggcccgacc gccgtctgag
     5821 gcggcccggc cggaccgccg gggcccggtt ctcccctgcc gggccttggg gggcgttcac
     5881 ggccggcacg gctcgcgacg gggcagaacg cggtagcgtg acaacagccc cgcggcgcca
     5941 cccgtttcac gggtggtgcg cggggcgcgg caccggacag gacagggcgg cactgggcag
     6001 gctcagtgcc ggcggagccg ccagtcgctc gacggtgccg acgccgtcga cagggccgtc
     6061 cggcccgatg agtggtagcg aaggatgggg gcagcacgtg gccgaggaag aagcgacgtt
     6121 cgccgaggag accggcgttt ccgaccggtt acgccaggac ctcccgcaca gcgcgcggat
     6181 gtacgactac tacctgggcg gcaagaccaa ctacgcggtg gaccggaccg cggccgagga
     6241 cgtgatccgc cggttcccct cgatccggac cgtggcccag atcaaccgcg ccttcgtgca
     6301 ccgctcggcg cgcttcctcg cccgcgagcg cgggatactc cagttcatcg acatcggcac
     6361 cggtatcccc acccagccga acctgcacga ggtcgtgcag cgcgaggtcc cgcaggcgcg
     6421 gatcgtctac gccgacaacg acccgatcgt gctggtctac gccgacaacc tgctgagcag
     6481 ttcgccgcag ggcgccaccg agtacgtcga ggcggacgtc accgacccgc agaagctgct
     6541 cgccgccgtg gaggcggccg gctgcctgga cttcacgcag ccggtcggcc tgagcctgaa
     6601 cgcggtgctg cacttcgtga cggacgcgat ggacgcctac ggtgtggtgc ggacgctggt
     6661 cgaccggctg gcgccgggtt cgtacctggc gatctcccac tgcaccccgg acttcgaccc
     6721 cggtgcctgg gcggcgatca cggacatcta caccgagggc ggcaccccgt tgcaggtccg
     6781 ctcgaaggcc gaggtggaac ggttcttcga gggcctggag ctggtggagc cgggcgtcga
     6841 ggtggcccac cggtggcggc cggaggccgg cgagggcccc accctggtca ccgacgcgca
     6901 gtcgtcgctg tacgcgggcg tggcccgcaa ggcctgagcc ggaccggcac cggtcggcgc
     6961 cgaggccggg gaccccgcgc aaaggggtcc ccggcctcga aggcacggct cggcgcgtcc
     7021 gccccggcaa gccgcgccgg aaaggaccta gcagaacttc ttgatgtggt cctggatgcg
     7081 gcggaccgtc gcgcgctggc cgagcgaggc gatcatcagc cggtcgaagc ccttgacgtg
     7141 ctcgtcgacc tcgtgctcct cctggaggta gaggccgccg ccggagcgct cgatgtagac
     7201 gatgtcgggg agctggccgt cggcgaagtc gaagatcgtc gtggtgccga tctgcaccgg
     7261 caggttcggc ttggtcaact ccaccaggtt gaagccgatg tcggggtcgt cggccaggga
     7321 tatcaggtgt tcgagctggc ggcgcatgcc ctcgcgggag ccgtacgggc ggaccagggt
     7381 cacctcgtcc agcacgcaca gcagcttctt gccgggctcg cgcatcagcc gctgctgccg
     7441 catcgcgcgc agctcgatga tccgctcgat ctcctgctcg ctcttgcccg gggcgttggc
     7501 cctgatcagc gcgcgggtgt actcggcggt ctgcaccagg cccggtagct gggcgatctc
     7561 gtagctgcgg atgcgctggg ccatgtcctc cagactgacc agggtctgga gggtcttggt
     7621 ggagacgtcg ctccacgcgt cccaccagcc gcgctcgttg gcggcgcggg cgaggtcgag
     7681 cagcaggtcg cgctccccct ggtcgtgcac gccgtagacg tccagcaggc gccggaggtc
     7741 ggcctccttg aagtcgtgcc ggccgttctc catccggctg atcttcgagg cggaatggcc
     7801 gatctggcgc acgacgtcct cggcggtgag gccgagcgcc tgacggcgct gcctcagcgt
     7861 ctcgccgaag atcatccgtt gcgccgtcgg ggcccgcccg gacagatcgt ggacgttacg
     7921 ggccccacgc ggactgcgag acggttgagc tggcacggtc ggtctccttc tcggggggtc
     7981 acaccccgta cgggtcacct atcgtgccgt agcgctcacg aagcggaggg cccgacgtca
     8041 catctggctg gatctcgcta ctcgacggcg aactcgccgc gcacgacccc ggtcacgaac
     8101 gcacgcatcg cggtgcggtc cacggtcagg atcggaccgt tcctgtcctt ggagtcgcgg
     8161 atcccgatcg ttccgtcgcc cagctccgcc acctcgacgc aattgctctc gtggttgctg
     8221 taggaactct tccgccaact caccgcggtt ccggccacgt ccggccggaa ccgcggagca
     8281 acgtggcgct gggcaccttc gccctgaccg gtcatcgttc tcctcctcgc agtgggggtg
     8341 tcggagcacg gccacatgtg caattgcatg cggtggccat ctttaataat ggcctggcac
     8401 cacgtgtttc atgcgtcgca ggggcgtact cccgttccca ccgcacgact tgggccacgg
     8461 cggcgccgcg tcagggcgcg ggcggggtga cggcgggggt caggagggcg aggcggtcgc
     8521 gggtggcctt cgggagtccg gcgaccacgc aggcgtagga gtgcgccagc aggtcgtcga
     8581 tctcgtccgg ggggatgctg ccgtcgagcg ccacggtgtt ccagtgcttc ttgttcaggt
     8641 ggtacccggg ggtgacggcc gcgtagcggt cccgcaggtg cagcgcgagg gccgggtcgc
     8701 acttgaggga gacggacggc gggtcggcgt ccgggctgag gatcgcgaag atccgtccac
     8761 cgaccttgta gacgtccgcg cccgggccga acgggttctc ctccgtggtc tccggcaggt
     8821 ccagggcgag ttcggcgagc gcttcgggct ccaccggcgg gaacggggag gcgtcggcgg
     8881 acgggtccgg ggagcccttc gcacccggtg ccccctccgc gtccggggac gcgtccgggg
     8941 acgcgcccgg ccggtcgccg cggccgccgt caccgtcgcg tgcgctggtc gccatcggtc
     9001 cgtcctcccg ggttccgcga gtgcgggcgc gccgcgggtc ggcgcgccct cacgctaccg
     9061 cccggcaccg acaccggccc ggggcggcgg ccggaagcgg cgcccggaag cagcgacctg
     9121 gagccgcggc cgggatcggc gaccgggggg aagcgccgag gccggccggc cgcttcggca
     9181 tcagccctgg cccgcgcccg ggcggcggac ctccaggtcg gtgcactgcc agctcagctc
     9241 gcgcaccccc ggacccggcg ccgagtgccc gtaggccccc gccgagacgc tgatgcacac
     9301 caccagccac gcgcgccacc gggtcccgac gccgtgcccg tcgtcgtaga cggtccggcc
     9361 gtcgacctgc cagcggacgc tgcgggcgcc gagcaggacg tcgagccgga aggggcggcc
     9421 gggcgtgacc agcccgtcga cgttgtcgcc ggcgccgccg acgtggttgc tcagttcgag
     9481 catgtgcgga tgggtcgggt ggtactcgaa ggcgtccact tctccgtggc cgggctgcgg
     9541 gcgcccggag gagccgtccc gtccccacgt ccacagcgcg ggccaggcgc ccggggtgtc
     9601 gtccacgacg caggtcgccg acagctcgtc gccgggcagc agttcgaagc cgccgggggc
     9661 gtactcggtg gagaccaggt cggtgtgcca cagcccgccg cccgcggcgc gggcccggaa
     9721 gacccccgcg gcggtcggcc cgtaggaggg gccgatctgg tcgagtttgt tgtcaccggg
     9781 gttgcggccg tcgggcgggt acgcggagga gcggcccacc gcccatgcct tgcggtcggt
     9841 gaagggggcg gcgaagacga cgggcgccgg acgcgctgcg gactgcggga cggtcatggg
     9901 tgcactccag gacggcggcc attacaccca gtgaacgccg atcacgcggg gtccgggtag
     9961 cggcgcgcgg aggcgccccc ggcgcgcaca ccgcgcggag cacgccccgg gccgtccgac
    10021 acggagccgg gccggtatcg ggacggcatc ggtacgacac cggaaccgga accggcggcg
    10081 gggccgcgcg gcacggcggc cggcccgctc ccgccagagc atgggagtca agcgtacgat
    10141 cagacaaaac cacgccaacg gtacgaacag ggccgagggg cggcgcgcac ccggcgcgag
    10201 cgggcacgga cgaaagggct cggatgggcg gcgcacgcga caacccggct gaacaccggg
    10261 acgacgacgc cgagctccgg cagatcgagg cgctcaaccg gctgcgggcg aacccttcgg
    10321 ccggacccga cccggccccc ggcgcttccg acgcctcccc cgcccccggg tcggaggccg
    10381 cggccggacc ggccccgtcc ggccacccgt ccggaccccc gtcgacgtcg cggccgcagg
    10441 agccgcacga tccgcacgcc ggacccggcg ggccggatgc cctccccccg caggacgcgc
    10501 cgggcacccc gcgcggtccg ggcgggtccg gggtccgcga cgcgcacggg cccgccgacg
    10561 cgccggtgga gccggcgccc gccgcccgta ccagcccccg gcaccgcgac gccctcctcg
    10621 tggacaccgg ccacaccgtc gccgacgcgc agtccctgga cgaggcgctc aaggcgctca
    10681 ccgccctgcg cgcgccgagc ttccccctcg acgggcaggc cgtcttcggc atcgacggca
    10741 ccttcctcac caagctcgcc acgctcggct tccgcgcggc cggccccgag cggatcttcc
    10801 ggatgccgct ggccaccgg
//
