LOCUS       NZ_CP064192            12841 bp    DNA     circular CON 30-JAN-2024
DEFINITION  Saccharothrix sp. 6-C chromosome, complete genome.
ACCESSION   NZ_CP064192
VERSION     NZ_CP064192.1
KEYWORDS    RefSeq.
SOURCE      Saccharothrix sp. 6-C
  ORGANISM  Saccharothrix sp. 6-C
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Saccharothrix.
REFERENCE   1  (bases 1 to 9045220)
  AUTHORS   Feng,S.
  TITLE     Saccharothrix texasensis strain 6-C
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9045220)
  AUTHORS   Feng,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2020) College of Life Sciences, Chongqing
            University, University Town Road, Chongqing, Chongqing 401331, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 10:20:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,982
            CDSs (total)                      :: 7,916
            Genes (coding)                    :: 7,819
            CDSs (with protein)               :: 7,819
            Genes (RNA)                       :: 66
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 41 of 97
            Pseudo Genes (incomplete)         :: 78 of 97
            Pseudo Genes (internal stop)      :: 6 of 97
            Pseudo Genes (multiple problems)  :: 27 of 97
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:34:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4508717
            Orig. end                         :: 4521558
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP064192.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12841
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="21"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12841
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12841
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7841](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7841)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            477..1430
                     /locus_tag="IOD16_RS18900"
                     /old_locus_tag="IOD16_18805"
     CDS             477..1430
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /locus_tag="IOD16_RS18900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IOD16_18805"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_236595095.1"
                     /sec_met_domain="Abi (E-value: 2.9e-14, bitscore: 52.1,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDDQTGPDPHHPVTAPHPAGSPSSSPQRPDSGPTTPPLAAPPPP
                     GAQPGGFQPGAHQPGEFPPVAQQPVAPPHSPVPAREVRAGLSLVGVFLAGQMTMLLVSV
                     LVLLAYGSADVGLLEDGRLLALLVVPTSLAAVVAVVGTAWFGGGPRLGRAARELAVRWN
                     VKEIAIGLALGLGGLALTLPAAAVWAAWVGEDQASSAVGDAFAGRQLAPAAAVVVFLAV
                     WLIAPLAEEVLFRGVLWRAFEHLGWNRWVVFAATSLVFSIAHLELLRTPLLLVLSIPIG
                     LARLFTGNLLASVVAHQVNNFLPAVALLLATTGALP"
     gene            complement(1440..1589)
                     /locus_tag="IOD16_RS18905"
     CDS             complement(1440..1589)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IOD16_RS18905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_201441112.1"
                     /transl_table=11
                     /translation="MEGQEHRRYLVCGIGVRLEDDLLVTAMGNEVLSGALPIDAAGVEA
                     WAAR"
     misc_feature    1543..1558
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.5"
                     /tool="antismash"
     gene            complement(2285..2953)
                     /locus_tag="IOD16_RS18910"
                     /old_locus_tag="IOD16_18815"
     CDS             complement(2285..2953)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803437.1"
                     /locus_tag="IOD16_RS18910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IOD16_18815"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_201441113.1"
                     /transl_table=11
                     /translation="MSPSTISPVAAGASLRGTVEDAVAAAIVSGELAPGTLVTAPTLAA
                     RFGVSATPVREAMLTLQKRGFVDVVRNKGFRITDVSEQDLWEIVRLRQQLEAPPMREIA
                     RTTQPSTVAELRLKAQAIVDAAAAADLATYLAADLDFHLRLLELHGNRRLVAIVKDLRQ
                     QTRMVGLAGMVGTTELARSAAEHHSLVDLLEARDGRGAEALLTDHIGHVLGWWSGNSED
                     "
     misc_feature    2991..3006
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.86"
                     /tool="antismash"
     misc_feature    2996..3006
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.4"
                     /tool="antismash"
     misc_feature    complement(3011..3027)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.76"
                     /tool="antismash"
     gene            complement(3069..4070)
                     /locus_tag="IOD16_RS18915"
                     /old_locus_tag="IOD16_18820"
     CDS             complement(3069..4070)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803439.1"
                     /locus_tag="IOD16_RS18915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IOD16_18820"
                     /product="proline racemase family protein"
                     /protein_id="WP_201441114.1"
                     /transl_table=11
                     /translation="MRASRVFSAVDSHTEGMPTRVVTGGFGVIPGATMNERRLHFVEHL
                     DHLRRLLVNEPRGHSAMSGAILQPPTRPDADFGVLYIEVSGCLPMCGHGTIGVATVLVE
                     TGMVEVTEPVTTIRLDTPAGLVVAEVAVRDGHADSVTLRNVPSYCDRLDEVVDVPGLGA
                     VRYSLAFGGNFYAMVDLPEVGLPFDRARKGEILAAGLAIMAAVNASAPPSHPEIEGVDH
                     CHHVEFIAPGSDARHSRHAMAIHPGWFDRSPCGTGTSARMAELHARGELPLEQDFVNES
                     FIGSRFTGCLVGETTVGGRPAVVPTVTGRAWVTGVGQYLLDPTDPYPAGFEF"
     gene            complement(4072..5004)
                     /locus_tag="IOD16_RS18920"
                     /old_locus_tag="IOD16_18825"
     CDS             complement(4072..5004)
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803440.1"
                     /locus_tag="IOD16_RS18920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IOD16_18825"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_201441115.1"
                     /transl_table=11
                     /translation="MSSAPAPLRGVIVATALPFKENPSAPAGLEVDFDRFAEHCDWLMD
                     NGCHGVGPNGSLGEYSSLTDAERRQVVRVAVDAVGDRGVVVAGVHGPGWHQARHWAELA
                     AEDGAHGVLALPPTMYRASRAQVVEHYTKIDEVGLPIMVYNNPVDTKVDLVPDLVAEIA
                     ALPNVAAVKEFSGDVRRLFAIAELCDIDVVVGADDVLFELMADGAVGWFAGFPNALPAD
                     CVALYEHLRAGRLTEARELYKHLVALFRWDSRTEFVQAVKLGMDEVGRYGGPCRPPRGP
                     LTAEQVGLVRADVRRAVAGVEALRAVEVA"
     gene            complement(5001..6407)
                     /locus_tag="IOD16_RS18925"
                     /old_locus_tag="IOD16_18830"
     CDS             complement(5001..6407)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803441.1"
                     /locus_tag="IOD16_RS18925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IOD16_18830"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_201441116.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 8.1e-26, bitscore:
                     89.8, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="DAO (E-value: 3.3e-07, bitscore: 29.1,
                     seeds: 521, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 8.1e-11, bitscore:
                     41.0, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDVLVVGGGPAGMAAALAARRHGAEHVVLLEAGDDVGGQYWRHP
                     PARRVDPDEGKWHHGWQRFLDLRSALRRDPRCEVVTNAHVWSVDRRDGAPPLVHALIGE
                     VDGQARVPRTFDPAALVLATGAQERTLPFPGWDLPGVVTAGAAQALAKGDRVAVGRRVL
                     VTGSGPFLLPVVSSLVRIGARVVGVVEAAGAARLARGWGAKPWRLAATGGKAAELAGYL
                     RVLAAHRIPYRVGAAVVAAHGDDRVEAVTVARLTPDWSPVPGTQRRVEVDAVCVGHGFV
                     PRAELAISAGCALGEDGAVEVDDRQRTSVPGVFTAGELTGIGGVDLALAEGLVAGAAAA
                     GGEPAPAALRRRAVFREFATRLAAAHAVGSGGRSWADDATVVCRCEDVTAGALRRAAEL
                     TGSKGLRPLKLTTRAGLGLCQGRTCGPAVEDLLTGVLDPGRTAHRPIAVPVRLGELADH
                     DRTGNREDVP"
     gene            complement(6400..6657)
                     /locus_tag="IOD16_RS18930"
                     /old_locus_tag="IOD16_18835"
     CDS             complement(6400..6657)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803442.1"
                     /locus_tag="IOD16_RS18930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IOD16_18835"
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="WP_201441117.1"
                     /sec_met_domain="Fer2_4 (E-value: 4.8e-21, bitscore: 73.5,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRVRIEVDGSPVEGLAGQSIAALLLATGRTSWRRTRTGRPRGVF
                     CGIGTCFDCVVTVNGLRDVRACRRRAADGDLVELQEDDRA"
     gene            complement(6657..7841)
                     /locus_tag="IOD16_RS18935"
                     /old_locus_tag="IOD16_18840"
     CDS             complement(6657..7841)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225094.1"
                     /locus_tag="IOD16_RS18935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="IOD16_18840"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_236595096.1"
                     /sec_met_domain="DAO (E-value: 9.8e-62, bitscore: 208.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSASLRVVVVGAGIVGAAIARALAREGCAVTVVDRGPAAGGTSAG
                     GEGNLLVSDKEPGAELRLAQRSLASWLRIEDELRDELPEGFPGLELERKGGLVVATTEM
                     GAAALEEFADAQRAAGVRAEPVDDARVRELEPDLTPHRTAAVLYPEDMQVQPVIATEAL
                     LAAARRHGALVRPGVEVTGPVLGRGGLVGVDTTDGVLLADAVVLAAGPWSGAVAERLGV
                     VLPVAPRRGTVLVTSRMPRRVGHKVYDADYVGAVGSGAADLQVSSVVESTAAGTVLIGS
                     SRERRGFDERIEVRVLAALAAKALLLFPFLADVQVMRAYGGFRPFVPDHLPVIGPDHRL
                     PGLWHATGHEGAGIGLSLATAEILRDQVLHGEDPSAAEFRLDRPTLLPHLGRAA"
     misc_feature    7843..7859
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.28"
                     /tool="antismash"
     misc_feature    7847..7857
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.07"
                     /tool="antismash"
     misc_feature    7853..7868
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.79"
                     /tool="antismash"
     gene            complement(7913..8944)
                     /locus_tag="IOD16_RS18940"
                     /old_locus_tag="IOD16_18845"
     CDS             complement(7913..8944)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IOD16_RS18940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="IOD16_18845"
                     /product="hypothetical protein"
                     /protein_id="WP_201441118.1"
                     /transl_table=11
                     /translation="MPTLRCVLLCVAVVLPAVPVLPAAAGQPPTPPGAGEVTWSVVPAA
                     ADGPDGRRVVDLEVGSGQRVTEHIAVTNHSPRPVVFALDANDGYLTGTGVFDMRSAEVV
                     PVDGGSWITLPDAVTVEGGATTVVPVVVTAPATATPGDHPAGVTASLDTLSGQVKVRNR
                     VGVRFNIRVTGDVTTALAVSDVQATYEQTWNPFAPGTVRLRHDVTNHGNVRVAAEIGVT
                     SSTLFADTTWTDRGTARSREVLPGGTRRFETRDTRAWPFGRVTTTVTVTPGPADGRPDL
                     PDATPVTVTVTTWALPVPQAALLALATLVVVAARFRTRTRRRRLEQLLARAREEGRAEA
                     ADA"
     gene            complement(9009..9629)
                     /locus_tag="IOD16_RS18945"
                     /old_locus_tag="IOD16_18850"
     CDS             complement(9009..9629)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IOD16_RS18945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="IOD16_18850"
                     /product="hypothetical protein"
                     /protein_id="WP_201441119.1"
                     /transl_table=11
                     /translation="MRRPTPTRLLAASALGVALCAAAVQPAAADDPGIDIDVVIEDLGT
                     PGSLTLTVAANDGVTLTENGSNASARQFTGVTPTITVTDTRDPADLPAGSFWAVVGQAG
                     AFTPAGGGTGFGAEHLGWRPRLLSDTTTGSVAAGEPVSSVVADGSGAPAVGLLGQELLV
                     STANAADETGSWDVNADLVLRTPVDVTAGQYTSTLTLSLFDQS"
     misc_feature    10294..10308
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.09"
                     /tool="antismash"
     misc_feature    complement(10367..10380)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.49"
                     /tool="antismash"
     misc_feature    complement(11875..11889)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.14"
                     /tool="antismash"
     misc_feature    complement(12154..12168)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.55"
                     /tool="antismash"
ORIGIN
        1 gctggtgtcg cggtactcgc gctgggagcg gcccacgtcc gacgacggcg gtgagggctg
       61 gctgaactgg gtcgtgctgc gggacgagga cggcgtcgcg gtcggcaccg tgcaggcgac
      121 caccacgacc ggcgtgaacg ggctggccgc cgaggtggcg tgggtgatcg gggtgccgtt
      181 ccagggtcac ggctacgcca ccgaggccgc cgcggccgtg gtggggtggc tggtcgacgg
      241 cggtgcgcgg gacgtgcacg cccacatcgc gcccgaccac ccggcgagcg aggcggtcgc
      301 gcggcgcatc gggatgcgcg ccaccggcga gttccgcgac gacggcgagc agcgctggcg
      361 gtggcgaccc gcccagggcc accggaacgc cacctgaccc cggtcgttgg gtctcgatca
      421 ccgcacgacc gcggcgctcg agttgaccgg acccgcgtcg cgggttatcc acggttgtga
      481 gcgatgacca gaccgggccg gacccccacc accccgtgac cgcgccccac ccggccgggt
      541 caccctcctc ctcaccccag cggcccgact ccggaccgac caccccgcca ctggccgcac
      601 ccccgccgcc cggggcccag ccgggcgggt tccagccggg cgcgcaccag ccgggcgagt
      661 tcccgccggt cgcgcagcag ccggtcgcgc cgcctcactc ccccgttccc gcccgggaag
      721 tccgagcggg gctgtcgttg gtcggcgtgt tcctcgccgg gcagatgacg atgctgctgg
      781 tgtccgtgct cgtgctgctg gcctacggct cggcggacgt ggggctgctc gaagacgggc
      841 ggctgctggc gctgctcgtc gtgcccacct cgttggccgc ggtcgtggcg gtggtcggca
      901 cggcctggtt cggcggtggg cccaggctcg ggcgcgcggc gcgcgagttg gcggtccggt
      961 ggaacgtcaa ggagatcgcc atcggcttgg cgctcggtct gggcggtttg gcgctcacgc
     1021 tccccgcggc cgccgtgtgg gccgcgtggg tcggtgagga ccaggccagc tcggcggtcg
     1081 gtgacgcgtt cgccggacgt caactggcgc cggcggcggc cgtggtcgtg ttcctggcgg
     1141 tgtggctgat cgcgccgctc gccgaggagg tgctgttccg gggtgtgctg tggcgcgcgt
     1201 tcgaacacct gggctggaac cgctgggtcg tgttcgcggc gaccagcctc gtgttctcga
     1261 tcgcccacct ggagctgctg cgcaccccgc tgctgctggt gctctccatc ccgatcggcc
     1321 tggcccggct gttcaccggc aacctgctgg cgagcgtcgt cgcccaccag gtcaacaact
     1381 tcctgcccgc cgtcgccctg ctcctggcca ccaccggcgc cctgccgtga cggggcgcgt
     1441 caccgggcgg cccacgcctc gacgcccgcg gcgtcgatcg gcagcgcgcc cgacaggacc
     1501 tcgttgccca tggcggtgac cagcaggtcg tcctccaggc gcacgccgat gccgcacacc
     1561 aggtagcggc ggtgctcctg gccctccaag gcgagcgccg accggggtgg tgcgggtgac
     1621 gtcggccggg tggagggagc gggtctccac gcccatgtcc agcagcagcg cgtcgccggg
     1681 ccgcaccggc ccgtcgcagc ggacccggtg caggatcgga gagcacggcg agcagccgtt
     1741 ccgggtcgcc ggcggcccgg tcgtcgtagt cggccgcggg ccgcacgggc aggtccagcg
     1801 cggtcgacca ggcggcgatc ccggccgacc ggccgaccca cagctcgccg tgggcggcac
     1861 tggcgtggaa gtcgcgctcg ccgggacggg ccggcggggt caggtggacg aagtcgctgt
     1921 cggcgcggaa cgcgtagttc gcgccgccgt tgcggggcgt ggccctcggc cgggacgatc
     1981 gtgtgctgcg acagctcggc gctgagccgg gcgcggtgct cggtggcggc gtcgaccggg
     2041 ccgggacctc gcggtcggcc cagccctgcc cgatgcgggc caggaagccc gggctgtcgt
     2101 cgccaggcga gccggccggt cacgcagggc gatcgagcag tgaccgcgat aacctgaggc
     2161 gacagtagta tgtcacacct cattcctggt atcgaagcga tatgccgccc gcggtggcga
     2221 cggagctccg gacagcacga cgggcccgcc gtgaggccgg cgggcccggt ggacgtgccc
     2281 ggtctcagtc ctcggagttg ccgctccacc agccgagcac gtggccgatg tggtcggtca
     2341 gcagcgcttc cgcgccccgc ccgtccctcg cctccagcag gtcgaccagg ctgtggtgct
     2401 cggccgcgga cctggccagc tcggtcgtgc cgaccatgcc ggccaacccg accatgcggg
     2461 tctgctggcg caggtccttc acgatggcga cgagcctgcg gttgccgtgc agctccagca
     2521 gccgcaggtg gaagtccagg tcggcggcga ggtaggtggc caggtcggcc gcggcggcgg
     2581 cgtccacgat ggcctgggcc ttgagccgca gctcggccac cgtcgagggc tgcgtcgtcc
     2641 tggcgatctc gcgcatcggc ggcgcctcca gctgctggcg cagccgcacg atctcccaga
     2701 ggtcctgctc gctgacgtcg gtgatgcgga agcccttgtt gcgcacgacg tcgacgaacc
     2761 cgcgcttctg cagggtgagc atggcctcgc ggaccggggt ggcggacacc ccgaagcggg
     2821 cggccagggt cggcgcggtg accagggtcc ccggcgccag ctcgccggag acgatggccg
     2881 cggcgaccgc gtcctccacc gtgccgcgca ggctcgcgcc cgcggccacc gggctgatgg
     2941 ttgacggact caccggtacg gcctcccgca gtcgtcgggc gtgtgagcct ggcactgcaa
     3001 tgtcacacga tgccgacttt accggtagga ggctgacgag catcgcaccc cgcgcccgcg
     3061 cgcccggatc agaactcgaa acccgccggg tacgggtcgg tcgggtcgag caggtactgc
     3121 ccgaccccgg tgacccaggc gcgcccggtg acggtcggca ccacggcggg ccgcccgccg
     3181 acggtcgtct cccccaccag gcagccggtg aaccggctgc cgatgaagga ctcgttgacg
     3241 aagtcctgct ccagcggcag ctcgccgcgc gcgtgcagct cggccatccg cgcggacgtg
     3301 cccgtcccgc acggcgagcg gtcgaaccag ccggggtgga tggccatcgc gtgccgcgag
     3361 tgccgcgcgt ccgagccggg cgcgatgaac tcgacgtggt ggcagtggtc gacgccctcg
     3421 atctccgggt ggctcggcgg cgcggaggcg ttgacggccg ccatgatcgc cagtccggcg
     3481 gcgaggatct cgcccttgcg ggcccggtcg aacggcagcc ccacctcggg gaggtcgacc
     3541 atcgcgtaga agttgccgcc gaacgccagg ctgtagcgga ccgcgcccag ccccggcacg
     3601 tcgacgacct cgtccagccg gtcgcagtac gacgggacgt tgcgcagggt cacgctgtcg
     3661 gcgtgcccgt cgcggaccgc cacctcggcc accaccagcc cggccggggt gtccagccgg
     3721 atcgtggtca ccggctcggt cacctcgacc atgccggtct cgaccaggac cgtggcgacg
     3781 ccgatggtgc cgtgcccgca catgggcagg cagccggaga cctcgatgta gagcacgccg
     3841 aagtccgcgt cggggcgcgt gggcggctgc aggatcgcgc cgctcatcgc cgagtggccg
     3901 cgcggttcgt tgaccagcag gcgcctcagg tggtcgaggt gctcgacgaa gtgcagccgc
     3961 cgctcgttca tggtggcgcc ggggatcacc ccgaagccgc cggtcaccac ccgggtcggc
     4021 atcccctcgg tgtgcgagtc gaccgcggag aacaccctcg acgcgcgcac ctcaggccac
     4081 ctcgaccgca cgcagcgcct cgaccccggc gacggcccgg cgcacgtccg cgcgcaccag
     4141 tccgacctgc tccgcggtga gcgggccgcg tggcgggcgg cacggtccgc cgtagcggcc
     4201 gacctcgtcc atgccgagct tgacggcctg cacgaactcg gtgcgcgagt cccagcggaa
     4261 cagcgcgacg aggtgcttgt acaactccct ggcctcggtg aggcggcccg cgcgcaggtg
     4321 ctcgtagagg gccacgcagt cggcgggcag ggcgttgggg aagcccgcga accagccgac
     4381 ggcgccgtcg gccatcagct cgaagagcac gtcgtcggcg cccacgacca cgtcgatgtc
     4441 gcacagctcg gcgatcgcga agagccggcg cacgtcgccg ctgaactcct tgacggcggc
     4501 cacgttgggc agcgccgcga tctcggcgac caggtcgggc accaggtcga ccttggtgtc
     4561 gaccgggttg ttgtagacca tgatcggcag gccgacctcg tcgatcttgg tgtagtgctc
     4621 gaccacctgc gcgcggctcg cccggtacat ggtgggaggc agggcgagca cgccgtgggc
     4681 gccgtcctcg gcggccagct ccgcccagtg cctggcctgg tgccagccgg ggccgtgcac
     4741 gcccgccacg acgacgccgc ggtcgcccac cgcgtcgacg gcgacccgca ccacctggcg
     4801 gcgctcggcg tcggtcaggg aggagtactc gccgagcgag ccgttggggc cgacgccgtg
     4861 gcagccgttg tccatcagcc agtcgcagtg ctcggcgaag cggtcgaagt cgacctccag
     4921 accggctggc gccgacgggt tctccttgaa cggcagggcg gtggcgacga tgaccccgcg
     4981 caggggagcc ggtgcggaac tcacgggacg tcctctcggt tgccggtgcg gtcgtggtcg
     5041 gcgagctcac cgaggcgcac cggtacggcg atggggcggt gggcggtgcg gccggggtcg
     5101 agcacgccgg tgagcaggtc ctcgacggcc gggccgcagg tgcggccctg gcacaggccg
     5161 agccccgcgc gggtggtcag cttgagcggg cgcaggccct tcgaaccggt cagctcggcg
     5221 gctcggcgca gggctccggc ggtgacgtcc tcgcagcggc agaccacggt ggcgtcgtcc
     5281 gcccaggacc gcccgcccga cccgacggcg tgggcggcgg cgagccgggt ggcgaactcg
     5341 cggaagaccg cgcgacggcg cagggcggcg ggcgcgggtt ctcccccggc ggccgcggcc
     5401 ccggcgacca ggccctcggc gagcgccagg tcgacgccgc cgatgccggt cagctcaccc
     5461 gccgtgaaga cgccgggcac ggaggtgcgc tgccggtcgt cgacctcgac ggcgccgtcc
     5521 tcgccgagcg cgcagccggc cgagatggcc agctcggcgc gtgggacgaa gccgtggccg
     5581 acgcagaccg cgtcgacttc gacgcgccgc tgcgtgccgg ggaccggtga ccagtcgggg
     5641 gtcagccggg cgacggtgac ggcctcgacg cggtcgtcgc cgtgcgcggc gacgacggcc
     5701 gcgcccaccc ggtaggggat gcggtgggcg gcgaggaccc gcaggtagcc cgccagctcg
     5761 gcggccttgc cgccggtggc ggccagtcgc cacggcttgg cgccccagcc cctggcgagc
     5821 cgcgcggcgc cggctgcctc gaccacgccg acgacgcgcg cgccgatgcg gaccaggctc
     5881 gacacgacgg gcagcaggaa cgggccggag cccgtgacga gcacgcgtcg gcccacggcc
     5941 acccggtcgc ccttggccag cgcctgcgcg gcgcccgcgg tgacgacgcc gggcaggtcc
     6001 cagccgggga acggcagggt ccgctcctgc gcgccggtgg cgaggaccaa cgcggcgggg
     6061 tcgaaggtgc gcggcacgcg ggcctggccg tcgacctcgc cgatgagggc gtgcacgagc
     6121 gggggcgcgc cgtcgcggcg gtcgacggac cagacgtggg cgttcgtgac gacctcgcac
     6181 cgggggtcgc gccgcagggc gctccgcagg tcgaggaagc gctgccagcc gtggtgccac
     6241 ttcccctcgt cggggtcgac gcgccgggcg ggcgggtgcc gccagtactg gccgccgacg
     6301 tcgtcgcccg cttcgagcag gacgacgtgc tccgcgccgt gccggcgggc ggcgagcgcg
     6361 gcggccatcc cggcgggtcc gccgccgacc accaggacgt caggcacggt cgtcttcctg
     6421 gagctcgacg aggtcgccgt cggcggcacg acgtcggcag gcccgcacgt cgcgcaggcc
     6481 gttgacggtg acgacgcagt cgaagcaggt gccgatgccg cagaacaccc cgcgcgggcg
     6541 gcccgtgcgg gtgcgccgcc aggaggtgcg gccggtggcg agcaggagcg cggcgatgct
     6601 ctgccccgcc aggccctcga ccgggctccc gtcgacctcg atgcggaccc gcgtcatcag
     6661 gcggcccttc cgaggtgcgg gagcagggtc gggcggtcga ggcggaactc ggcggccgac
     6721 gggtcttcgc cgtgcaggac ctggtcgcgc aggatctccg cggtggccag gctcaggccg
     6781 atgcccgcgc cctcgtgacc ggtggcgtgc cacaggccgg gcagccggtg gtcggggccg
     6841 atgacgggca ggtggtcggg gacgaacggg cggaagccgc cgtaggcgcg catgacctgg
     6901 acgtcggcga ggaacgggaa cagcagcagc gccttggcgg cgagcgcggc gagcacccgg
     6961 acctcgatcc gctcgtcgaa cccgcgccgc tcgcgggagg agccgatgag gacggtgccc
     7021 gcggccgtcg actcgaccac gctggagacc tgcaggtcgg ccgcgccgga gccgaccgcg
     7081 cccacgtagt cggcgtcgta gaccttgtgc ccgacccgcc ggggcatccg ggaggtcacc
     7141 agcacggtgc cccgccgggg cgcgaccggc agcacgaccc cgaggcgctc ggcgaccgcg
     7201 ccggaccagg ggccggccgc gagcacgacc gcgtcggcga gcagcacgcc gtcggtggtg
     7261 tcgaccccga cgagcccgcc ccggccgagc accggcccgg tgacctcgac gcccgggcgc
     7321 accagggcgc cgtgacgacg ggcggcggcg agcagcgcct cggtggcgat gacgggctgc
     7381 acctgcatgt cctctgggta gagcacggcg gcggtgcggt gcggggtgag gtccggttcg
     7441 agctcgcgga cgcgggcgtc gtcgaccggc tcggctcgga ccccggcggc ccgctgggcg
     7501 tcggcgaact cttcgagggc cgcggctccc atctcggtgg tggcgaccac gagaccgccc
     7561 ttgcgctcca gctccagacc ggggaagccc tcgggcagct cgtcgcgcag ctcgtcctcg
     7621 atgcgcagcc aggaggcgag cgagcgctgc gcgagccgga gttccgcgcc gggctccttg
     7681 tcggagacga gcaggttccc ctcaccgccg gcggaggtgc cgccggcggc gggtccgcgg
     7741 tccacgacgg tgaccgcgca gccctcgcgg gccagcgccc gggcgatcgc cgcgccgacg
     7801 atccccgccc ccaccacgac cacccgaaga gaagcggaca cgtcagtacc atgtcacatg
     7861 gcgctcacct tgggaagctt cccgatcttc cgcccgtcgt gccgcatgcc cctcaggcgt
     7921 cggccgcttc ggcgcgcccc tcctcgcggg cgcgggcgag gagctgctcg agacgacgac
     7981 gccgggtgcg ggtccggaac ctggcggcga cgaccaccag cgtcgcgaga gccagcaggg
     8041 ccgcttgggg cacgggcagc gcccaggtgg tgacggtgac ggtcaccggg gtcgcgtcgg
     8101 gcaggtcggg acggccgtcg gcggggccgg gggtcacggt gacggtggtg gtgacgcggc
     8161 cgaagggcca ggccctggtg tcgcgggtct cgaaccggcg ggtcccgccg gggaggacct
     8221 cgcgggagcg ggcggtgccc cgatcggtcc acgtggtgtc ggcgaagaga gtggagctgg
     8281 tcacgccgat ctcggcggcg acgcggacgt ttccgtggtt ggtgacgtcg tgccgcaggc
     8341 gcaccgtgcc gggggcgaag gggttccagg tctgctcgta ggtggcctgg acgtcggaga
     8401 cggcgagggc ggtggtgacg tcgccggtga ccctgatgtt gaagcgcacc ccgacccggt
     8461 tgcgcacctt cacctgaccg gagagcgtgt ccagcgacgc ggtgacaccc gccgggtggt
     8521 cacccggcgt ggcggtggcc ggagcggtga ccacgacggg gacgacggtg gtggcgccgc
     8581 cctcgacggt cacggcgtcg ggcagggtga tccaggagcc cccgtcgacc gggacgacct
     8641 cggccgagcg catgtcgaag acgcccgtgc cggtgaggta gccgtcgttg gcgtcgaggg
     8701 cgaagaccac gggccggggc gagtggttgg tgaccgcgat gtgctcggtg acccgctgac
     8761 cggaccccac ttcgaggtcg accacgcggc ggccgtccgg cccgtccgcc gcggcgggga
     8821 cgaccgacca ggtcacctcc cccgcgccgg gcggcgtcgg gggttgtccc gccgcggccg
     8881 ggaggacggg gacggccggc aggaccacgg cgacgcacag caacacgcag cggagggtgg
     8941 gcatcgggtc ctccgggagg agtgccgggg tccgggacgc ggcagccgcg tcccggaccg
     9001 ggccgacttc aggactggtc gaacagggac agggtcagcg tcgaggtgta ctggcccgcg
     9061 gtcacgtcga ccggcgtgcg cagcaccagg tccgcgttga cgtcccacga gccggtctcg
     9121 tccgccgcgt tcgccgtcga caccagcagc tcctggccga gcaggccgac cgccggcgcg
     9181 ccggagccgt cggcgacgac gctggagacc ggttcgccgg cggccaccga gccggtggtc
     9241 gtgtcggaga gcaggcgcgg gcgccaaccc aggtgctccg cgccgaaccc ggtgccgccg
     9301 cccgcggggg tgaacgcgcc ggcctggcct accaccgccc agaacgagcc cgccgggagg
     9361 tccgccgggt cacgggtgtc ggtgacggtg atcgtgggtg tgacgccggt gaactgccgg
     9421 gccgaggcgt tcgagccgtt ctcggtcagg gtcacgccgt cgttcgccgc caccgtcagc
     9481 gtgagcgagc ccggtgtgcc caggtcctcg atcaccacgt cgatgtcgat gcccgggtcg
     9541 tcggccgccg ccggctggac ggccgccgcg cacagcgcga cgcccagcgc cgaggccgcg
     9601 agcagccggg tcggggttgg tcttctcatc gtgggtgctc ctcgggggtt cagcggcagg
     9661 tgtgggcggg gtgctgcgcg gtgatccgcg cggtgaccgg tcggccgtcg acgacaccgg
     9721 tggcggtgac cgtcgccgtc ccggcgggca cggcggtcag gcgggtgttg aacgcctggt
     9781 acgcggactt gccgggcgcc acgtcggacg cggtgcgcgt gccgtgcggc gtgaccagct
     9841 ccacggtcag cggcacgtcg tcgtcgttgc gggcggtgac cgacacgtac gcgttggcgc
     9901 cgatgcactg ggcacgcgcc tcgacgtgca cgtcccaggt cgcgccgccg tcgcccacga
     9961 ccagcgtcag cgtggtccgc gaggtgccgg ccccgcccac gtggtcgggc aggacgagga
    10021 cgccgtcgag cacgtgccgg cccggtgtgc ccacgacggc cgggtcgaag tcccagcgga
    10081 cgccgacggc ctggttgtcc ctggagccgt cgttgaacga gacctcgacc ttctccggca
    10141 ggtggggcag ctcccccacc gcgacggtcc gctcgaagga ctgggcgctc tggacggtga
    10201 tgccgcccgg ccccatgtcg ggccgcacgt acaccttcgc ctcggcgatg agcccgtagg
    10261 cgtcattggt gccgtagacg gtgaacgggt cggtgttggg ctcggccacc atctccgggg
    10321 tgatcggctg ccaggtgaac gccacctcgc cgcgggcgcc gctcgcgtag accccgtcca
    10381 gcccgtcggg cagcgccggc gcctgtccca ccgtggtgcg gacgagcacc ggcgacgcca
    10441 ccgactcgac ggtctcgccg ttggcgcgcc accgcagcac gcccgtgccc gcgccttcgc
    10501 cctggacgcc ccagacgcgc acgcgcaggt ggctcgccgc gacgggctgg aaggcgaacc
    10561 ggttgtaggc gccgacggcc agtcccccgg cgtaggtgga gccgtcggtc aacgtgaccg
    10621 gggtccacgt ggcgccgtcg tccgaggact cgatggcgta ggtggtggcc gtcggcctgc
    10681 gggtgccgcc gttgtcgtcg taccagtaga ggtccgtcga ggacagccgc accggcgagg
    10741 tccaccggta ctggatccac gcctcccgct cggggctggt gccgttgcgg gtctggcccc
    10801 agttgcccca gccgttgccc gcacccgggc tggagctcgc cggggtggtg ccctccttga
    10861 cccgctcgtg gttctcccac gacgcggtcc cgctggtggt gacggtcgcg gaggcgccga
    10921 actcggcggt gacgtccttc tccgcgaggg tcgccgcgac ggtggcctcc cgccgctgga
    10981 cgccgtcgtc ggcgaagaac cggaactcgt agtcgccggc gaccgtgccg gtgaccctgg
    11041 tggcgagcgc gcccgcgtcg gcgaagatga cgcccgcgcc gtccggcttg gcgacggtct
    11101 cccagccgta ggtcagctcg ctgtcgtacg gcacgccgtc gtcgagagcc gtgccgacca
    11161 accgggtgga caggttgccg tccgccgagg cgtcccgggt cgcggtgacc accggcgcct
    11221 ggttggcgac gtccggcacc tcccggcccg agtcgaacac ctggatctcg gagatggcgg
    11281 tgtagtgccc cggcgcgttg gagaacgcga cgcgcacctt gcccgccgtg acggtgtcga
    11341 acagcgcctc gttgaacttc gccgagggca gcgccgggtt cttgaactgg tccggcacct
    11401 cgtgccagcc gccgtcgccg tcgggcagct gcacccacca gcgggccggc tcccggtacc
    11461 caccggcctg gcggtcgctg acgaaggtca ccttgacgtt gtcgaaggac ttcgccgccc
    11521 cgaggtcgag ttcgacgtgg ccgttcttct ccgtggcgcc gtggttgccc cagtagggct
    11581 cgttggcggt gttgccgtcg gtcaccgcgg ccagtgtgac cggtcgctcc agctccttgg
    11641 tggcgccggg ggtgtggttg atcgtggacg cgctccagcc cggcgtgtgg aactgccgcc
    11701 agggagcggg tcgcgcgccc tgctgggtgt gggagctgct cagcgcggcg ccggtggcca
    11761 ggttcggggc gtcctcggtg aggtcgatgc ccgccgtctt caggtagtcg acgacccggc
    11821 cgtcctggat cccggtgtcc accgcgctgg ggaactcggc cccggtggac gcggtgaagg
    11881 tgacggtcag cccgtcctcg gcgacgacct cgttggcgcc ggggtcgtag gtgagcttgc
    11941 cgatccggtc ggtgctgacc ttcttctccc cgttgaggaa caggctgtag cccgcgccca
    12001 gcccgtactc ggtgccgtcc gggtcccaca ccacggtgac gtcctgcccg tggtagcgca
    12061 ggttgtcgac catgaagtgg tcgtggccga ggtcgatcgg cgacagctcc accttgtcgt
    12121 cggaccgcgg ctgcaggccc gccatgtcct cgatgaagat gtagttcatg ttgccgagca
    12181 tcacgtggta cgggttgttg cggttgtagg tcttcgtggt cgcgttccag ttcgagtagt
    12241 actcggcctg gttcgccacc cgcgcgtcac cgcccgggta gatgctccac gccatccagt
    12301 ccagcaggcg cttggcgtag gccggctcga cgtacttccg ctcggggtcg tagtggcgca
    12361 gcgccgaccg cacaccgcgg tactgcacgg tgaagttgat gttggagaag ttgttcgagc
    12421 cgccgatgcc gtaggccgcg cggtcgtact ggtcggcggt gtagaacggg aagatcggga
    12481 agttgtcgcc gtaggtcagg aaccggaacc cgtcgacgta cttctcccac tggtcgaagg
    12541 ggatcaggtt ctgggcgtac acgtcgtaga ggttcgactc cttcgccggg atcaggtccc
    12601 gctccgcctc cggcagcggg ttggcccgtc cgttggagct cgccgccgag gtggcgccgt
    12661 gcgaggtgcc cgcgaggaac atccgcgtgt ccgggctcca caggcggttc aggatcgcgt
    12721 cgcggacccg gtcggccgac gcgccgaccc ggtcgacctt cgcctgctcg gcgcccgcga
    12781 tccggtacag ctcgcgggcc gcgtcgaacg cgccccacac gtacgccgac tcgggccgct
    12841 c
//
