GCF_016591715.1 - 12 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_AP024109.1 (Mesorhizobium sp. J8)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NAPAA	168,759	202,829
Region 2	RRE-containing	405,058	425,486
Region 3	lassopeptide	605,243	627,859
Region 4	RRE-containing	633,228	653,515	Low	salecan	saccharide
Region 5	hserlactone	933,663	954,301
Region 6	hserlactone	1,606,961	1,627,593
Region 7	hserlactone	1,699,498	1,720,130	Low	succinoglycan	saccharide
Region 8	T3PKS	2,549,601	2,590,668
Region 9	terpene	4,032,625	4,053,488
Region 10	redox-cofactor	4,729,762	4,751,955
Region 11	hserlactone	5,078,296	5,098,910
Region 12	terpene-precursor	6,201,841	6,222,716

NZ_AP024109.1 - Region 1 - NAPAA

Download region GenBank file

Download region SVG

Location: 168,759 - 202,829 nt. (total: 34,071 nt) Show pHMM detection rules used

NAPAA: (AMP-binding and TIGR02353)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase oxidoreductase

MJ8_RS00925: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

MJ8_RS00945: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

MJ8_RS00990: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

MJ8_RS01015: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: PPTase

MJ8_RS00955: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

Ligases: ligase

MJ8_RS00965: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.73	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001135.5	0.61	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001249.5	0.60	NRPS	dimethylcoprogen	Alternaria alternata
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001240.4	0.60	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata
BGC0001641.4	0.60	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.73	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001135.5	0.61	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001249.5	0.60	NRPS	dimethylcoprogen	Alternaria alternata
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001240.4	0.60	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata
BGC0001641.4	0.60	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 25.95 out of 29.51

Confidence: medium

Additional weak hits (10):

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.12 out of 26.12

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.08 out of 31.73

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.52 out of 26.12

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.32 out of 30.22

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.69 out of 26.06

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.20 out of 26.12

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 20.88 out of 26.12

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.26 out of 26.06

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.60 out of 30.98

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.82 out of 29.65

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 168758 - 202829:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MJ8_RS00965: X

AMP-binding (53..442): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FVAMFDFSSEEVACLVGGELNTYGPTEATVTATM
Nearest Stachelhaus code(s):: OH-Orn DGEAVGGVTK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
DosR	Hypoxia stress response regulator

NZ_AP024109.1 - Region 2 - RRE-containing

Download region GenBank file

Download region SVG

Location: 405,058 - 425,486 nt. (total: 20,429 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: glucosidase hydrolase

MJ8_RS02005: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

MJ8_RS02070: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

Translocases: transporter

MJ8_RS02085: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002285.2	0.47	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0000737.3	0.47	saccharide	capsular polysaccharide	Pasteurella multocida
BGC0000741.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000744.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000745.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000740.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000746.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000747.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000748.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000739.3	0.45	saccharide	capsular polysaccharide	Pasteurella multocida

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002285.2	0.47	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0000737.3	0.47	saccharide	capsular polysaccharide	Pasteurella multocida
BGC0000741.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000744.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000745.3	0.46	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000740.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000746.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000747.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000748.3	0.45	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000739.3	0.45	saccharide	capsular polysaccharide	Pasteurella multocida

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 19.86 out of 21.84

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.23 out of 19.52

Confidence: medium

Additional weak hits (5):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 19.15 out of 31.73

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.34 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.45 out of 29.38

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 19.20 out of 31.73

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 10 - 79; Score: 35.9

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
AfsR	Pleiotropic regulatory for antibiotic production
MexT	Global virulence regulator

NZ_AP024109.1 - Region 3 - lassopeptide

Download region GenBank file

Download region SVG

Location: 605,243 - 627,859 nt. (total: 22,617 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

MJ8_RS02990: nucleotide sugar dehydrogenase

smcogs: SMCOG1152: nucleotide sugar dehydrogenase

Transferases: acyltransferase glycosyltransferase

MJ8_RS02920: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

MJ8_RS02930: sugar transferase

smcogs: SMCOG1146: sugar transferase

Hydrolases: hydrolase

MJ8_RS02950: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Ligases: synthase

MJ8_RS03005: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001634.3	0.53	ribosomal	keywimysin	Streptomyces sp. NRRL F-5702
BGC0001356.4	0.51	ribosomal	paeninodin	Paenibacillus dendritiformis C454
BGC0000740.3	0.50	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000746.3	0.50	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000747.3	0.50	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000748.3	0.50	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000741.3	0.49	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000744.3	0.49	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000745.3	0.49	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000572.5	0.48	ribosomal	capistruin	Burkholderia thailandensis E444

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001634.3	0.53	ribosomal	keywimysin	Streptomyces sp. NRRL F-5702
BGC0001356.4	0.51	ribosomal	paeninodin	Paenibacillus dendritiformis C454
BGC0000740.3	0.50	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000746.3	0.50	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000747.3	0.50	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000748.3	0.50	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000741.3	0.49	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000744.3	0.49	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000745.3	0.49	saccharide	capsular polysaccharide	Streptococcus agalactiae
BGC0000572.5	0.48	ribosomal	capistruin	Burkholderia thailandensis E444

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptide(s) for MJ8_RS02965

MJ8_RS02965

Leader:

MEQNVEKHEYETPSLTVHGSIETITQ

Full core:

GGAGTTALDASFPAHTPVGELTFS

Putative macrolactam:

GGAGTTALD

antiSMASH-DB 4.0 matches for MJ8_RS02965

100.0%

NZ_AP024109.1and 2 others:

NZ_CP083956.1 Lassopeptides: FJ974_RS01165_lassopeptideNZ_FOVT01000001.1 Lassopeptides: F3H96_RS01735_lassopeptide

Lassopeptides: MJ8_RS02965_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

GGAGTTALDASFPAHTPVGELTFS

1

GGAGTTALDASFPAHTPVGELTFS

24

95.8%

NZ_ATYO01000004.1:

Lassopeptides: YU3_RS40500_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

GGAG TALDASFPAHTPVGELTFS

1

GGAGVTALDASFPAHTPVGELTFS

24

95.8%

NZ_CP083951.1:

Lassopeptides: FJ430_RS01235_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

GGAGTTALDASFPAHTP+GELTFS

1

GGAGTTALDASFPAHTPIGELTFS

24

with 88 more sequence matches

Only the first 10 are displayed here.

91.7%

NZ_CP034445.1and 2 others:

NZ_CP034446.1 Lassopeptides: EJ069_RS21680_lassopeptideNZ_CP034449.1 Lassopeptides: EJ072_RS11465_lassopeptide

Lassopeptides: EJ068_RS18530_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

GGAG+TALD SFPAHTPVGELTFS

1

GGAGSTALDVSFPAHTPVGELTFS

24

87.5%

NZ_CP015318.1:

Lassopeptides: A6B35_RS34290_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

GG G+TALDASFPAHTP+GELTFS

1

GGGGSTALDASFPAHTPIGELTFS

24

87.5%

NZ_CP034455.1:

Lassopeptides: EJ078_RS12825_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

G +GTTALDA+FPAHTPVGELTFS

1

GASGTTALDAAFPAHTPVGELTFS

24

83.3%

NZ_CP034444.1and 2 others:

NZ_CP034447.1 Lassopeptides: EJ070_RS06315_lassopeptideNZ_CP034451.1 Lassopeptides: EJ074_RS13805_lassopeptide

Lassopeptides: EJ067_RS13990_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

GGAGTTALDA+FPAHTP +LTFS

1

GGAGTTALDAAFPAHTPFDQLTFS

24

83.3%

NZ_CP034448.1:

Lassopeptides: EJ071_RS01775_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

G +GTTALDA+FPAHTPVG+LTFS

1

GASGTTALDAAFPAHTPVGDLTFS

24

83.3%

NZ_CP080524.1:

Lassopeptides: LHFGNBLO_RS06050_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

GG G+TALDA+FPAHTP+GELTFS

1

GGGGSTALDAAFPAHTPIGELTFS

24

83.3%

NZ_ML703270.1:

Lassopeptides: C7I87_RS19195_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

GG G TALDASFPAHTP+G+LTFS

1

GGGGNTALDASFPAHTPIGDLTFS

24

79.2%

NC_002678.2and 27 others:

NC_014923.1 Lassopeptides: MESCI_RS01205_lassopeptideNC_019973.1 Lassopeptides: MESAU_RS01210_lassopeptideNZ_AP023242.1 Lassopeptides: JN279_RS01270_lassopeptideNZ_AP023243.1 Lassopeptides: JG746_RS01220_lassopeptideNZ_AP023246.1 Lassopeptides: MesoLj_RS01255_lassopeptideNZ_AP023254.1 Lassopeptides: JG747_RS01230_lassopeptideNZ_AXAE01000013.1 Lassopeptides: A3AU_RS1000000137730_lassopeptideNZ_AXAL01000027.1 Lassopeptides: A3A9_RS43075_lassopeptideNZ_CP015062.1 Lassopeptides: A4R28_RS34150_lassopeptideNZ_CP015064.1 Lassopeptides: A4R29_RS32555_lassopeptideNZ_CP021070.1 Lassopeptides: A9K65_RS01210_lassopeptideNZ_CP033361.1 Lassopeptides: EB233_RS01335_lassopeptideNZ_CP033365.1 Lassopeptides: EB231_RS01220_lassopeptideNZ_CP033507.1 Lassopeptides: EB229_RS01240_lassopeptideNZ_CP034453.1 Lassopeptides: EJ076_RS07385_lassopeptideNZ_CP050296.1 Lassopeptides: HB778_RS17925_lassopeptideNZ_CP080525.1 Lassopeptides: BPNPMPFG_RS25760_lassopeptideNZ_CP088147.1 Lassopeptides: LRP29_RS01210_lassopeptideNZ_CP097252.1 Lassopeptides: M5D98_RS01245_lassopeptideNZ_JALJRL010000001.1 Lassopeptides: NR193_RS01610_lassopeptideNZ_KB913026.1 Lassopeptides: MESLO3_RS1000000141745_lassopeptideNZ_KI632510.1 Lassopeptides: MESLO_RS32475_lassopeptideNZ_KI911320.1 Lassopeptides: M729_RS1000000138215_lassopeptideNZ_KI912159.1 Lassopeptides: MESLO2_RS1000000139130_lassopeptideNZ_KI912610.1 Lassopeptides: MESCI2_RS1000000137645_lassopeptideNZ_QGGH01000004.1 Lassopeptides: C8D77_RS17730_lassopeptideNZ_RZRU01000003.1 Lassopeptides: EOA16_RS05930_lassopeptide

Lassopeptides: MAFF_RS37875_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

GG G+TA+DASFPAHTP+G+LTFS

1

GGGGSTAIDASFPAHTPIGDLTFS

24

79.2%

NC_015675.1and 4 others:

NZ_CP034779.1 Lassopeptides: EJ079_RS03975_lassopeptideNZ_JANIFC010000015.1 Lassopeptides: NP503_RS29205_lassopeptideNZ_LMCP01000023.1 Lassopeptides: ASD12_RS33300_lassopeptideNZ_LMHO01000001.1 Lassopeptides: ASD99_RS31725_lassopeptide

Lassopeptides: MESOP_RS33200_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

GG G TA+DASFPAHTP+G+LTFS

1

GGGGNTAIDASFPAHTPIGDLTFS

24

79.2%

NZ_AP023247.1:

Lassopeptides: JG743_RS01195_lassopeptide

1

GGAGTTALDASFPAHTPVGELTFS

24

GG+GTTALDA+FPAHTP +LTFS

1

GGSGTTALDAAFPAHTPFDQLTFS

24

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.22 out of 18.46

Confidence: strong

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.59 out of 25.88

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.11 out of 27.78

Confidence: medium

Additional weak hits (10):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.79 out of 26.85

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.76 out of 27.78

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.99 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.51 out of 27.78

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.85 out of 36.53

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.15 out of 26.06

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.04 out of 39.62

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.17 out of 18.57

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.87 out of 32.93

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.44 out of 29.65

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

MJ8_RS02965 - Class II: Cleavage pHMM score: -0.30; RODEO score: 24; Monoisotopic mass: 2285.1 Da; Molecular weight: 2286.4 Da; Number of bridges: 0

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 12 - 92; Score: 88.1

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
PsrA	Oleic acid responsive regulator
RpoN	Regulator of nitrogen assimilation and virulence

NZ_AP024109.1 - Region 4 - RRE-containing

Download region GenBank file

Download region SVG

Location: 633,228 - 653,515 nt. (total: 20,288 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase transferase

MJ8_RS03045: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

MJ8_RS03060: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

Ligases: ligase synthase

MJ8_RS03040: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

MJ8_RS03065: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000749.3	0.55	saccharide	capsular polysaccharide	Escherichia coli
BGC0001380.3	0.50	saccharide	salecan	Agrobacterium sp. ZX09
BGC0001765.3	0.48	saccharide	salecan	Agrobacterium tumefaciens
BGC0002674.2	0.46	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0001550.3	0.44	ribosomal	citrulassin D	Streptomyces katrae
BGC0000763.5	0.44	saccharide	stewartan	Pantoea stewartii subsp. stewartii DC283
BGC0000791.3	0.43	saccharide	O-antigen	Cronobacter sakazakii
BGC0002825.2	0.42	ribosomal	nocapeptin A	Nocardia terpenica IFM 0406
BGC0000787.3	0.42	saccharide	O-antigen	Escherichia coli
BGC0000728.5	0.42	saccharide	capsular polysaccharide	Klebsiella pneumoniae subsp. ozaenae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000749.3	0.55	saccharide	capsular polysaccharide	Escherichia coli
BGC0001380.3	0.50	saccharide	salecan	Agrobacterium sp. ZX09
BGC0001765.3	0.48	saccharide	salecan	Agrobacterium tumefaciens
BGC0002674.2	0.46	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0001550.3	0.44	ribosomal	citrulassin D	Streptomyces katrae
BGC0000763.5	0.44	saccharide	stewartan	Pantoea stewartii subsp. stewartii DC283
BGC0000791.3	0.43	saccharide	O-antigen	Cronobacter sakazakii
BGC0002825.2	0.42	ribosomal	nocapeptin A	Nocardia terpenica IFM 0406
BGC0000787.3	0.42	saccharide	O-antigen	Escherichia coli
BGC0000728.5	0.42	saccharide	capsular polysaccharide	Klebsiella pneumoniae subsp. ozaenae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.62 out of 30.25

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.44 out of 30.22

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.22 out of 20.01

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 7 - 90; Score: 49.3; RRE type: PqqD_RRE; Location: 25 - 90; Score: 28.9

TFBS definitions

Regulator	Description

NZ_AP024109.1 - Region 5 - hserlactone

Download region GenBank file

Download region SVG

Location: 933,663 - 954,301 nt. (total: 20,639 nt) Show pHMM detection rules used

hserlactone: Autoind_synth

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

MJ8_RS04420: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

Transferases: aminotransferase carbamoyltransferase

MJ8_RS04500: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

MJ8_RS04505: ornithine carbamoyltransferase

smcogs: SMCOG1114: ornithine carbamoyltransferase

Lyases: hydratase

MJ8_RS04470: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000852.5	0.48	other (other)	ectoine	Sporosarcina pasteurii
BGC0000858.3	0.47	other (other)	ectoine	Methylobacter marinus
BGC0000855.3	0.47	other (other)	ectoine	Methylomicrobium kenyense
BGC0000856.3	0.47	other (other)	ectoine	Methylophaga thalassica
BGC0000859.3	0.47	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000895.5	0.47	other (other)	citrulline	Lactobacillus plantarum subsp. plantarum
BGC0000860.3	0.46	other (other)	ectoine	Methylarcula marina
BGC0000857.3	0.46	other (other)	ectoine	Methylophaga alcalica
BGC0000853.3	0.46	other (other)	ectoine	Streptomyces anulatus
BGC0002052.3	0.46	other (ectoine)	ectoine	Streptomyces sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000852.5	0.48	other (other)	ectoine	Sporosarcina pasteurii
BGC0000858.3	0.47	other (other)	ectoine	Methylobacter marinus
BGC0000855.3	0.47	other (other)	ectoine	Methylomicrobium kenyense
BGC0000856.3	0.47	other (other)	ectoine	Methylophaga thalassica
BGC0000859.3	0.47	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000895.5	0.47	other (other)	citrulline	Lactobacillus plantarum subsp. plantarum
BGC0000860.3	0.46	other (other)	ectoine	Methylarcula marina
BGC0000857.3	0.46	other (other)	ectoine	Methylophaga alcalica
BGC0000853.3	0.46	other (other)	ectoine	Streptomyces anulatus
BGC0002052.3	0.46	other (ectoine)	ectoine	Streptomyces sp.

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.89 out of 29.72

Confidence: medium

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 18.06 out of 20.01

Confidence: medium

Additional weak hits (3):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.52 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.09 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 19.21 out of 27.78

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
ArgR	Regulator of arginine biosynthesis genes
NtrC	Nitrogen regulatory protein C

NZ_AP024109.1 - Region 6 - hserlactone

Download region GenBank file

Download region SVG

Location: 1,606,961 - 1,627,593 nt. (total: 20,633 nt) Show pHMM detection rules used

hserlactone: Autoind_synth

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

MJ8_RS07375: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

MJ8_RS07385: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

MJ8_RS07395: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: aminotransferase

MJ8_RS07340: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000812.3	0.45	other (other)	huperzine A	Shiraia sp. slf14
BGC0000841.5	0.43	other (non-nrp beta-lactam)	alanylclavam, 2-hydroxymethylclavam, 2-formyloxymethylclavam, clavam-2-carboxylate, clavulanic acid	Streptomyces clavuligerus ATCC 27064
BGC0000888.5	0.41	other (other)	bacilysin	Bacillus sp. CS93
BGC0001928.2	0.40	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0001197.5	0.39	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000843.5	0.38	other (non-nrp beta-lactam)	alanylclavam, 2-hydroxymethylclavam, 2-formyloxymethylclavam, clavam-2-carboxylate	Streptomyces clavuligerus ATCC 27064
BGC0001184.4	0.38	other (other)	bacilysin	Bacillus velezensis FZB42
BGC0000204.5	0.38	PKS	benastatin A, benastatin B, BE-43767, TAN1532B	Streptomyces sp. A2991200
BGC0001987.2	0.37	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis
BGC0001002.5	0.37	NRPS, PKS	jawsamycin	Streptomyces roseoverticillatus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000812.3	0.45	other (other)	huperzine A	Shiraia sp. slf14
BGC0000841.5	0.43	other (non-nrp beta-lactam)	alanylclavam, 2-hydroxymethylclavam, 2-formyloxymethylclavam, clavam-2-carboxylate, clavulanic acid	Streptomyces clavuligerus ATCC 27064
BGC0000888.5	0.41	other (other)	bacilysin	Bacillus sp. CS93
BGC0001928.2	0.40	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0001197.5	0.39	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000843.5	0.38	other (non-nrp beta-lactam)	alanylclavam, 2-hydroxymethylclavam, 2-formyloxymethylclavam, clavam-2-carboxylate	Streptomyces clavuligerus ATCC 27064
BGC0001184.4	0.38	other (other)	bacilysin	Bacillus velezensis FZB42
BGC0000204.5	0.38	PKS	benastatin A, benastatin B, BE-43767, TAN1532B	Streptomyces sp. A2991200
BGC0001987.2	0.37	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis
BGC0001002.5	0.37	NRPS, PKS	jawsamycin	Streptomyces roseoverticillatus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.93 out of 20.97

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.88 out of 27.55

Confidence: medium

Additional weak hits (6):

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.55 out of 26.12

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.04 out of 24.78

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.03 out of 25.88

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.36 out of 29.25

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.53 out of 25.88

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.72 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CcpA	Catabolite control protein A
GnfM	Regulator of nitrogen-fixation genes

NZ_AP024109.1 - Region 7 - hserlactone

Download region GenBank file

Download region SVG

Location: 1,699,498 - 1,720,130 nt. (total: 20,633 nt) Show pHMM detection rules used

hserlactone: Autoind_synth

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

MJ8_RS07780: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Ligases: synthase

MJ8_RS07690: 8-amino-7-oxononanoate synthase

smcogs: SMCOG1109: 8-amino-7-oxononanoate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001238.3	0.58	other (other)	biotin	Aspergillus nidulans
BGC0000802.5	0.56	saccharide	succinoglycan	Sinorhizobium meliloti
BGC0001239.3	0.55	other (other)	biotin	Aspergillus nidulans
BGC0002354.4	0.48	other (other)	huimycin	Kutzneria albida DSM 43870
BGC0001937.4	0.46	other (other)	tubercidin	Streptomyces tubercidicus
BGC0002362.3	0.45	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0000879.5	0.42	other (other)	toyocamycin, sangivamycin	Streptomyces rimosus
BGC0000881.3	0.39	other (other)	toyocamycin	Streptomyces ahygroscopicus subsp. wuzhouensis
BGC0001808.5	0.39	other (other)	toyocamycin	Streptomyces diastatochromogenes
BGC0001925.4	0.38	other (other)	altemicidin, SB-203207, SB-203208	Streptomyces sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001238.3	0.58	other (other)	biotin	Aspergillus nidulans
BGC0000802.5	0.56	saccharide	succinoglycan	Sinorhizobium meliloti
BGC0001239.3	0.55	other (other)	biotin	Aspergillus nidulans
BGC0002354.4	0.48	other (other)	huimycin	Kutzneria albida DSM 43870
BGC0001937.4	0.46	other (other)	tubercidin	Streptomyces tubercidicus
BGC0002362.3	0.45	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0000879.5	0.42	other (other)	toyocamycin, sangivamycin	Streptomyces rimosus
BGC0000881.3	0.39	other (other)	toyocamycin	Streptomyces ahygroscopicus subsp. wuzhouensis
BGC0001808.5	0.39	other (other)	toyocamycin	Streptomyces diastatochromogenes
BGC0001925.4	0.38	other (other)	altemicidin, SB-203207, SB-203208	Streptomyces sp.

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 21.73 out of 30.97

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 25.36 out of 35.63

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.29 out of 23.43

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_AP024109.1 - Region 8 - T3PKS

Download region GenBank file

Download region SVG

Location: 2,549,601 - 2,590,668 nt. (total: 41,068 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

MJ8_RS11875: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

MJ8_RS11895: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

Transferases: glycosyltransferase

MJ8_RS11885: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

Hydrolases: hydrolase

MJ8_RS11860: amidohydrolase

smcogs: SMCOG1244: amidohydrolase

Ligases: synthase

MJ8_RS11905: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001962.3	0.51	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0000902.4	0.50	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0002403.2	0.49	PKS	β-Diketones	Hordeum vulgare subsp. vulgare
BGC0000285.5	0.47	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0002332.2	0.46	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.
BGC0001077.4	0.44	PKS	6-anteisoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-geranyl-2-methoxyhydroquinone, 6-palmityl-4-O-geranyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-palmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone	Actinoplanes missouriensis 431
BGC0000840.5	0.43	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0002150.2	0.42	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.42	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0001687.3	0.42	other (other)	N-tetradecanoyl tyrosine	uncultured bacterium CSL12

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001962.3	0.51	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0000902.4	0.50	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0002403.2	0.49	PKS	β-Diketones	Hordeum vulgare subsp. vulgare
BGC0000285.5	0.47	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0002332.2	0.46	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.
BGC0001077.4	0.44	PKS	6-anteisoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-geranyl-2-methoxyhydroquinone, 6-palmityl-4-O-geranyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-palmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone	Actinoplanes missouriensis 431
BGC0000840.5	0.43	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0002150.2	0.42	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.42	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0001687.3	0.42	other (other)	N-tetradecanoyl tyrosine	uncultured bacterium CSL12

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.82 out of 18.46

Confidence: medium

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.58 out of 21.84

Confidence: medium

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 24.74 out of 27.55

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.19 out of 20.97

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 24.59 out of 30.25

Confidence: medium

Additional weak hits (9):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.23 out of 30.25

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.40 out of 26.12

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 19.79 out of 30.97

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 19.13 out of 30.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.92 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.32 out of 29.38

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.44 out of 36.54

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.17 out of 29.38

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 19.81 out of 33.14

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
ColR	Phenol-responsive regulator
GnfM	Regulator of nitrogen-fixation genes
MexT	Global virulence regulator
RpoN	Regulator of nitrogen assimilation and virulence

NZ_AP024109.1 - Region 9 - terpene

Download region GenBank file

Download region SVG

Location: 4,032,625 - 4,053,488 nt. (total: 20,864 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: dehydratase

MJ8_RS19505: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000678.5	0.33	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002120.3	0.28	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0001845.2	0.26	NRPS, PKS, other (aminocoumarin)	alpiniamide	Streptomyces sp. CBMAI 2042
BGC0000698.5	0.25	saccharide	hygromycin A	Streptomyces hygroscopicus
BGC0000964.5	0.24	NRPS, PKS	burkholdac A, burkholdac B	Burkholderia thailandensis E264
BGC0000001.5	0.22	PKS	abyssomicin C, atrop-abyssomicin C	Verrucosispora maris AB-18-032
BGC0000241.5	0.20	PKS, saccharide	lomaiviticin A, lomaiviticin C, lomaiviticin D, lomaiviticin E	Salinispora tropica CNB-440
BGC0000240.5	0.20	PKS, saccharide	lomaiviticin A, lomaiviticin C, lomaiviticin D, lomaiviticin E	Salinispora pacifica
BGC0001693.3	0.20	PKS	nenestatin	Micromonospora echinospora
BGC0000014.5	0.19	PKS	ambruticin	Sorangium cellulosum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000678.5	0.33	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002120.3	0.28	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0001845.2	0.26	NRPS, PKS, other (aminocoumarin)	alpiniamide	Streptomyces sp. CBMAI 2042
BGC0000698.5	0.25	saccharide	hygromycin A	Streptomyces hygroscopicus
BGC0000964.5	0.24	NRPS, PKS	burkholdac A, burkholdac B	Burkholderia thailandensis E264
BGC0000001.5	0.22	PKS	abyssomicin C, atrop-abyssomicin C	Verrucosispora maris AB-18-032
BGC0000241.5	0.20	PKS, saccharide	lomaiviticin A, lomaiviticin C, lomaiviticin D, lomaiviticin E	Salinispora tropica CNB-440
BGC0000240.5	0.20	PKS, saccharide	lomaiviticin A, lomaiviticin C, lomaiviticin D, lomaiviticin E	Salinispora pacifica
BGC0001693.3	0.20	PKS	nenestatin	Micromonospora echinospora
BGC0000014.5	0.19	PKS	ambruticin	Sorangium cellulosum

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	C30 carotenoid	diapophytoene	C30	acyclic	none
triterpene		PSPP	C30	acyclic	PP
triterpene		squalene	C30	acyclic	none
tetraterpene	C40 carotenoid	phytoene	C40	acyclic	none
triterpene		hydroxysqualene	C30	acyclic	OH

Genes with terpene domains:

MJ8_RS19475: PT_phytoene_like

Domain 1 (25 - 283): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → diapophytoene; FPP → PSPP; FPP → squalene; GGPP → phytoene; PSPP → hydroxysqualene

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.88 out of 20.97

Confidence: medium

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.19 out of 21.55

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.86 out of 27.78

Confidence: medium

Additional weak hits (8):

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.34 out of 20.97

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 21.68 out of 29.25

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.72 out of 20.97

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.49 out of 24.78

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.48 out of 29.65

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.19 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.57 out of 26.06

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PP	Diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
ArcA	Repressor of carbon oxidation
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria

NZ_AP024109.1 - Region 10 - redox-cofactor

Download region GenBank file

Download region SVG

Location: 4,729,762 - 4,751,955 nt. (total: 22,194 nt) Show pHMM detection rules used

redox-cofactor: (cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

MJ8_RS22725: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Lyases: dehydratase hydratase

MJ8_RS22715: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

MJ8_RS22720: L-carnitine dehydratase/bile acid-inducible

smcogs: SMCOG1189: L-carnitine dehydratase/bile acid-inducible

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002306.2	0.50	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001928.2	0.49	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0002493.3	0.43	NRPS	parabactin	Paracoccus denitrificans PD1222
BGC0002064.2	0.42	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10
BGC0002425.3	0.42	ribosomal	stlassin	Streptomyces sp.
BGC0002218.2	0.41	terpene	(-)-Cyatha-3,12-diene	Dentipellis sp. KUC8613
BGC0000671.3	0.41	terpene	momilactone B	Oryza sativa Japonica Group
BGC0000486.4	0.40	ribosomal	butyrivibriocin AR10	Butyrivibrio fibrisolvens
BGC0000519.4	0.40	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000218.5	0.40	PKS	doxorubicin	Streptomyces peucetius

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002306.2	0.50	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0001928.2	0.49	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0002493.3	0.43	NRPS	parabactin	Paracoccus denitrificans PD1222
BGC0002064.2	0.42	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10
BGC0002425.3	0.42	ribosomal	stlassin	Streptomyces sp.
BGC0002218.2	0.41	terpene	(-)-Cyatha-3,12-diene	Dentipellis sp. KUC8613
BGC0000671.3	0.41	terpene	momilactone B	Oryza sativa Japonica Group
BGC0000486.4	0.40	ribosomal	butyrivibriocin AR10	Butyrivibrio fibrisolvens
BGC0000519.4	0.40	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000218.5	0.40	PKS	doxorubicin	Streptomyces peucetius

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.55 out of 18.46

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.38 out of 20.97

Confidence: medium

Additional weak hits (7):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.51 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.03 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.76 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.80 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.66 out of 27.78

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.06 out of 20.01

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.50 out of 29.25

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: PqqD_RRE; Location: 10 - 98; Score: 91.4; RRE type: Thurincin_rSAM_RRE; Location: 36 - 92; Score: 27.9; RRE type: Stand_Alone_Lasso_RRE; Location: 45 - 98; Score: 27.4

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
RpoN	Regulator of nitrogen assimilation and virulence

NZ_AP024109.1 - Region 11 - hserlactone

Download region GenBank file

Download region SVG

Location: 5,078,296 - 5,098,910 nt. (total: 20,615 nt) Show pHMM detection rules used

hserlactone: Autoind_synth

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: transketolase

MJ8_RS24365: transketolase

smcogs: SMCOG1204: transketolase

Ligases: synthase

MJ8_RS24350: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

MJ8_RS24355: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002285.2	0.38	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001587.5	0.37	other (other)	ethylenediaminesuccinic acid hydroxyarginine (EDHA)	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001789.3	0.37	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000537.3	0.37	ribosomal	nisin U	Streptococcus uberis
BGC0000943.5	0.37	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0000488.4	0.37	ribosomal	circularin A	Clostridium beijerinckii
BGC0002568.2	0.35	other (other)	Ethylenediaminesuccinic acid hydroxyarginine	Streptomyces scabiei 87.22
BGC0000410.5	0.35	NRPS	pseudomonine	Pseudomonas fluorescens
BGC0002095.3	0.35	NRPS	leupeptin Pr, leupeptin Ac	Streptomyces roseus
BGC0001553.5	0.33	NRPS, PKS	clifednamide A	Streptomyces sp. JV178

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002285.2	0.38	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001587.5	0.37	other (other)	ethylenediaminesuccinic acid hydroxyarginine (EDHA)	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001789.3	0.37	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000537.3	0.37	ribosomal	nisin U	Streptococcus uberis
BGC0000943.5	0.37	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0000488.4	0.37	ribosomal	circularin A	Clostridium beijerinckii
BGC0002568.2	0.35	other (other)	Ethylenediaminesuccinic acid hydroxyarginine	Streptomyces scabiei 87.22
BGC0000410.5	0.35	NRPS	pseudomonine	Pseudomonas fluorescens
BGC0002095.3	0.35	NRPS	leupeptin Pr, leupeptin Ac	Streptomyces roseus
BGC0001553.5	0.33	NRPS, PKS	clifednamide A	Streptomyces sp. JV178

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.16 out of 26.33

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.25 out of 20.47

Confidence: medium

Additional weak hits (5):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.06 out of 21.55

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.21 out of 26.33

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.99 out of 23.43

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.11 out of 18.57

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.23 out of 20.01

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
AbrC3	Antibiotic production activator
VqsM	Regulator of quorum-sensing signalling systems

NZ_AP024109.1 - Region 12 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 6,201,841 - 6,222,716 nt. (total: 20,876 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

MJ8_RS29680: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: sulfotransferase

MJ8_RS29710: glutathione S-transferase

smcogs: SMCOG1193: glutathione S-transferase

Hydrolases: hydrolase

MJ8_RS29715: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

MJ8_RS29755: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: synthetase

MJ8_RS29745: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001164.3	0.55	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001616.3	0.50	NRPS, PKS	himeic acid A	Aspergillus japonicus
BGC0002397.2	0.46	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0000902.4	0.42	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0002387.2	0.41	PKS	yangpumicin A	Micromonospora yangpuensis
BGC0002496.3	0.41	NRPS	serratiochelin A, serratiochelin C, serratiochelin B	Serratia plymuthica
BGC0001605.3	0.41	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000666.5	0.41	terpene	xiamycin A	Streptomyces sp. SCSIO 02999
BGC0002007.2	0.41	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001164.3	0.55	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001616.3	0.50	NRPS, PKS	himeic acid A	Aspergillus japonicus
BGC0002397.2	0.46	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0000902.4	0.42	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0002387.2	0.41	PKS	yangpumicin A	Micromonospora yangpuensis
BGC0002496.3	0.41	NRPS	serratiochelin A, serratiochelin C, serratiochelin B	Serratia plymuthica
BGC0001605.3	0.41	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000666.5	0.41	terpene	xiamycin A	Streptomyces sp. SCSIO 02999
BGC0002007.2	0.41	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

MJ8_RS29745: PT_FPPS_like

Domain 1 (29 - 290): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: DMAPP, IPP → FPP

Transcription Factor Binding Sites

Binding sites found:

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 26.12 out of 26.12

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (6):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.97 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.27 out of 18.57

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.84 out of 36.54

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.55 out of 30.22

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.84 out of 32.93

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.84 out of 29.65

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
LuxO	Repressor of luminescence