LOCUS       NZ_AP024109            22194 bp    DNA     circular CON 10-NOV-2023
DEFINITION  Mesorhizobium sp. J8 chromosome, complete genome.
ACCESSION   NZ_AP024109
VERSION     NZ_AP024109.1
KEYWORDS    RefSeq.
SOURCE      Mesorhizobium sp. J8
  ORGANISM  Mesorhizobium sp. J8
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1
  AUTHORS   Kusaba,I., Nakao,T., Maita,H., Sato,S., Chijiiwa,R., Yamada,E.,
            Arima,S., Kojoma,M., Ishimaru,K., Akashi,R. and Suzuki,A.
  TITLE     Mesorhizobium sp. J8 can establish symbiosis with Glycyrrhiza
            uralensis, increasing glycyrrhizin production
  JOURNAL   Plant Biotechnol. 38, 57-66 (2021)
  REMARK    DOI:10.5511/plantbiotechnology.20.1124a
REFERENCE   2  (bases 1 to 6690860)
  AUTHORS   Maita,H. and Sato,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2020) Contact:Shusei Sato Tohoku University,
            Department of Environmental Life Sciences, Graduate School of Life
            Sciences; 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/10/2023 07:29:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,439
            CDSs (total)                      :: 6,376
            Genes (coding)                    :: 6,302
            CDSs (with protein)               :: 6,302
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 48 of 74
            Pseudo Genes (incomplete)         :: 41 of 74
            Pseudo Genes (internal stop)      :: 12 of 74
            Pseudo Genes (multiple problems)  :: 21 of 74
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:17:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4729761
            Orig. end                         :: 4751955
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024109.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22194
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="10"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22194
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22194
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12194](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12194
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     gene            complement(41..238)
                     /locus_tag="MJ8_RS22625"
     CDS             complement(41..238)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MJ8_RS22625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_225247995.1"
                     /transl_table=11
                     /translation="MPIRRQWFSVMRTDHVMSPAMATFNDFLMRKGATYLPLLGKLYPR
                     AEADEAARTQQARAPGTRRG"
     gene            328..504
                     /locus_tag="MJ8_RS22630"
                     /old_locus_tag="MJ8_45070"
     CDS             328..504
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024639.3"
                     /locus_tag="MJ8_RS22630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45070"
                     /product="HAD hydrolase-like protein"
                     /protein_id="WP_263649707.1"
                     /transl_table=11
                     /translation="MMVGDSISDVGAARAASMPIALLRGGYTHVPVEELDADLVCDSLL
                     DLPSAMQRLQAAA"
     gene            complement(644..1024)
                     /locus_tag="MJ8_RS22635"
                     /old_locus_tag="MJ8_45080"
     CDS             complement(644..1024)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876267.1"
                     /locus_tag="MJ8_RS22635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45080"
                     /product="cytochrome c family protein"
                     /protein_id="WP_201410939.1"
                     /transl_table=11
                     /translation="MRVIAFFAAIFLVTFITDESYAQDAAAGEKVFTKCKVCHVADKDQ
                     NKVGPSLNGVIGRTAGTHPGFSYSQAMVAAGKSGVKWDEPTLTTYLHDPKAMVKGTKMA
                     FVGLKDDKDVANVIAYLKQFSK"
     gene            1315..1524
                     /locus_tag="MJ8_RS22640"
                     /old_locus_tag="MJ8_45090"
     CDS             1315..1524
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532063.1"
                     /locus_tag="MJ8_RS22640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45090"
                     /product="hypothetical protein"
                     /protein_id="WP_201410940.1"
                     /transl_table=11
                     /translation="MNMVDLILTVCLSANPGACRDEHLYFESRGSLFQCMMLAPSEIAK
                     WSQEHPALKVRRWKCAFPTKDRAI"
     gene            1562..2551
                     /locus_tag="MJ8_RS22645"
                     /old_locus_tag="MJ8_45100"
     CDS             1562..2551
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532064.1"
                     /locus_tag="MJ8_RS22645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45100"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_201410941.1"
                     /transl_table=11
                     /translation="MMISRRETLRLAALGTAAVLGAQSIAAAASKVRIGVLKFGTVSWE
                     LDTLKQHSFDAANGIDLDVVNFAGEDATNVAMLAGAIDIIVTDWLWVSRQRSEGGDVTL
                     APYSTSVGAIMVEDASPIRTIADLKGKKIGVAGGALDKSWLLIQALARRDHGLDLPAVT
                     DVVFGAPPLISQKAIQGELDAALNFWHFCARLEAGGFRRLIGADGAQIALGASGPVSAL
                     GYVFHDKWANENPRAARGFLKASAQAKDLLARSDEEWLRLAPVIRADGKELAKLRDRYR
                     EGIPRRPVAEEAADAGKLYRVLAAIGGEKLVGSAPEMAPGTFWREPPQ"
     misc_feature    complement(2620..2633)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.55"
                     /tool="antismash"
     gene            2623..3375
                     /locus_tag="MJ8_RS22650"
                     /old_locus_tag="MJ8_45110"
     CDS             2623..3375
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1044: ABC
                     transporter, permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836349.1"
                     /locus_tag="MJ8_RS22650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45110"
                     /product="ABC transporter permease"
                     /protein_id="WP_225248322.1"
                     /transl_table=11
                     /translation="MLMPALTVTISLLGLGLLWSLAANAWPSRAFPGPGQVWQVLLREA
                     ASGDLFYHLGATFGRVAAAYLVAMIVGSVIGILLGSYRRADRFFNPWVILFLNIPALVI
                     IVLAYIWFGLNEAAAIGAVAVNKIPNVVVTMREGARALDPRYAEMAAVYRFGPLDRLRH
                     ILLPQLQPYLAAASRSGIALIWKIVLVVELLGRSNGVGFQIHLYFQLFDVAAILAYTLA
                     FVAVMLVIELLLVQPVERHATRWRRRPA"
     gene            3344..4084
                     /locus_tag="MJ8_RS22655"
                     /old_locus_tag="MJ8_45120"
     CDS             3344..4084
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532066.1"
                     /locus_tag="MJ8_RS22655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45120"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_201410942.1"
                     /transl_table=11
                     /translation="MQPVGAAAPLRVDIAEKTFRSAHGVSVTALKNLSFEVRQGEFVCL
                     LGPSGCGKTTTLRILLGLDRQFSGSFQLPGGGSNRIAAVFQEPTLLPWRTVEENVRLAL
                     PKSLRMKNLDALLDTLGLTGMRSLYPSELSLGLARRVALARAFAIEPAVLFLDEPFVSL
                     DERTAGQLRRLLLDVWSARPTTALMVTHNLTEALALSDRIIVLAPRPSHVLGVFDIGLP
                     RQHRSPEAANDLLRSFHQKFPGVT"
     gene            complement(4122..4475)
                     /locus_tag="MJ8_RS22660"
                     /old_locus_tag="MJ8_45130"
     CDS             complement(4122..4475)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532069.1"
                     /locus_tag="MJ8_RS22660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45130"
                     /product="hypothetical protein"
                     /protein_id="WP_201410943.1"
                     /transl_table=11
                     /translation="MVRYAGFSILTLLFSSATVFANPAGAAGDYPTATIVDYVLGCMRT
                     NGQTRQALESCSCSIDVIASILPYDHYERAETFKSMSLTTGENAGLFRESAPAKAARTE
                     LKQAQAEADVRCF"
     misc_feature    complement(4723..4737)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.51"
                     /tool="antismash"
     misc_feature    4742..4755
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(4747..4760)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.76"
                     /tool="antismash"
     misc_feature    4836..4850
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.8"
                     /tool="antismash"
     misc_feature    complement(5133..5144)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.38"
                     /tool="antismash"
     gene            5269..7089
                     /locus_tag="MJ8_RS22665"
                     /old_locus_tag="MJ8_45140"
     CDS             5269..7089
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836354.1"
                     /locus_tag="MJ8_RS22665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45140"
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="WP_263649708.1"
                     /transl_table=11
                     /translation="MAENPKDWVMQTGDYANTRYSTLKQITKDNVKNLQVKWTFSTGVL
                     RGHEGGPLIIGDVMYVHAPFPNTVYALDLNKDGKILWKYEPKQDTNVIPIMCCDTVNRG
                     VAYAPADGDRPALVILNQADTTVVALDAKTGKVVWSVKNGENTDGGKGESGTSAPVVVK
                     DKVLVGVSGAEFGVRGWLAAYDLKDGKLAWKAYSEGPDADTLLDPEKTTQLGKPVGKDS
                     GTNTWEGEQWKTGGGTTWGWYSYDPKLNLVYYGTGNPSTWNPVQRPGDNRWSMTIFARD
                     ADTGVAKWVYQMTPHDEWDYDGINEMILVDGMEVKGKKHDVLVHFDRNGFAYTMDRATG
                     ELLVAKKYDPAVNWATEVNMDPNSKEYGRPQVVAKYSTQQNGEDTNSTGICPAALGTKD
                     QQPATYSPKTGLFYVPTNHVCMDYEPYKVSYTAGQPYVGATVSMYPAPGGDGNMGNFIA
                     WDAAKGEIVWSKPEQFSVWSGALATAGDVIFYGTLEGYIKAVDEHGKELYKFKTPSGII
                     GNVTPFEHNGKQYIAVLSGVGGWAGIGLAGGLLSPDNAAAWHGAVDQGRAQGDQAAVVG
                     TAGLGAVGGYAALADYTTLGGQLTVFGLPD"
     misc_feature    complement(7238..7252)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.66"
                     /tool="antismash"
     misc_feature    complement(7245..7258)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.06"
                     /tool="antismash"
     gene            7248..7772
                     /locus_tag="MJ8_RS22670"
                     /old_locus_tag="MJ8_45150"
     CDS             7248..7772
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532071.1"
                     /locus_tag="MJ8_RS22670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45150"
                     /product="c-type cytochrome, methanol metabolism-related"
                     /protein_id="WP_201410945.1"
                     /transl_table=11
                     /translation="MFIRIAVSVLALTLLPLAGSISAQAEDSADKAKAVKDEGGKYLDA
                     DGNPTYKIENGTVDWYTFSGYRRYHSDCHVCHGQDGMGSTYAPALANSLKTIDYPTFLS
                     IVAQGRKNTANGKENVMPAFGDNKNVYCYLDDLYVYLRARADGAAPRGRPPKHEDKPQA
                     AKDAEKSCMGG"
     gene            7744..8646
                     /locus_tag="MJ8_RS22675"
                     /old_locus_tag="MJ8_45160"
     CDS             7744..8646
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532072.1"
                     /locus_tag="MJ8_RS22675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45160"
                     /product="substrate-binding domain-containing protein"
                     /protein_id="WP_201410946.1"
                     /transl_table=11
                     /translation="MPKNPAWEDDVTGRVASKPRFVLPLLGALALLPSAAWAQGAGLGA
                     AGELVDPDILRVCADPSNMPFTDQNGQGFENKLAELVARKTGRKSVAYTWFPTVIGFVR
                     NTLAANRCDVIMGYAQGDELVQNTNAYYRSSYVLVHPKGDGLEGVETIEDPKLAGKRIG
                     VVERTPPTANMAANKLLRTAKIYPLAVDTRVTPSMGEVMIKDMLAGKIDAAILWGPMAG
                     YYAKQLDANVAIVPLVKEKTGSRMSYRITMGVRPSDQEWKRTLNKVIRENQAEINKLLL
                     DYNVPLIDEHDNPITATPG"
     misc_feature    8743..8757
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.5"
                     /tool="antismash"
     gene            9072..10004
                     /gene="pqqB"
                     /locus_tag="MJ8_RS22680"
                     /old_locus_tag="MJ8_45170"
     CDS             9072..10004
                     /codon_start=1
                     /gene="pqqB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316541.1"
                     /locus_tag="MJ8_RS22680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45170"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="WP_201415540.1"
                     /transl_table=11
                     /translation="MRVKIVGSAAGGGFPQWNCNYRLSRAARAGVPGMRPRTQSSIAVS
                     ADGTGWVLFNASPDIRQQIAQAPELQPASDAPRRSTPIRAVVLTNADVDHVAGLLSLRE
                     RQPFAIYASAQVLATLEANSIFNVLDPAIVPRRLLAPTEEMTIRDAAGRETGVVIESFP
                     VPGKVALYLEDKDHPDADFSSDAGDTVGIRIAGSGGAVFYIPGCARIDAALRTRLAGAA
                     CLLFDGTVYTDGEMIAAGVGHKTGARMGHIAMSGQAGSIAGLADVKIGRRIFIHINNTN
                     PVLDENSAEHAAVKAAGWEVASDGMEMEF"
     gene            10001..10759
                     /gene="pqqC"
                     /locus_tag="MJ8_RS22685"
                     /old_locus_tag="MJ8_45180"
     CDS             10001..10759
                     /EC_number="1.3.3.11"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR02111"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532076.1"
                     /locus_tag="MJ8_RS22685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45180"
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="WP_201410947.1"
                     /sec_met_domain="TIGR02111 (E-value: 1.3e-101, bitscore:
                     337.4, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDFHDVARDGLSKRDLEAVLRRVGDERYHNRHPFHHRMTGGALS
                     KAEMQAWALNRYCYQAVIPRKDAMILARAEDPAFRAAWRKRIEDHDGEDGWSGGIARWL
                     HLATSLGLDAEAVKSERLALPATRFAVGAYLSFCTNRTLLEAVASSLTEMFSPRIIGER
                     VPAMLAKYDYITEDTLAYFTQRPEQAKRDADFALAYVLAHADTAERQQRAIDALVFKCD
                     ILWAMLDALQHAYGTPGNIPPGAFCPEAAL"
     gene            10756..11055
                     /gene="pqqD"
                     /locus_tag="MJ8_RS22690"
                     /old_locus_tag="MJ8_45190"
     CDS             10756..11055
                     /GO_function="GO:0048038 - quinone binding [Evidence IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PqqD_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Thurincin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316539.1"
                     /locus_tag="MJ8_RS22690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45190"
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="WP_225247996.1"
                     /sec_met_domain="PqqD_RRE (E-value: 7.8e-27, bitscore:
                     91.4, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.1e-14, bitscore: 52.9,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Thurincin_rSAM_RRE (E-value: 6.2e-07,
                     bitscore: 27.9, seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 8.9e-07,
                     bitscore: 27.4, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMTALRERAIVSSRSVPSLPRHVRIQYDPVRQAFAVLSPEKVFWP
                     NEISLDILRRCDGQSSVERIIADLAADYDADPQDVAADVVAFLQEWSDKLLVKL"
     aSDomain        10786..11049
                     /aSDomain="PqqD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing protein in a pyrroloquinoline
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_MJ8_RS22690_PqqD_RRE.1"
                     /evalue="3.40E-29"
                     /identifier="RREFam006.1"
                     /label="MJ8_RS22690"
                     /locus_tag="MJ8_RS22690"
                     /protein_end="98"
                     /protein_start="10"
                     /score="91.4"
                     /tool="antismash"
                     /translation="VSSRSVPSLPRHVRIQYDPVRQAFAVLSPEKVFWPNEISLDILRR
                     CDGQSSVERIIADLAADYDADPQDVAADVVAFLQEWSDKLLVK"
     aSDomain        10864..11031
                     /aSDomain="Thurincin_rSAM_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing rSAM protein in a thurincin
                     sactipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_MJ8_RS22690_Thurincin_rSAM_RRE.1"
                     /evalue="2.70E-09"
                     /identifier="RREFam019.1"
                     /label="MJ8_RS22690"
                     /locus_tag="MJ8_RS22690"
                     /protein_end="92"
                     /protein_start="36"
                     /score="27.9"
                     /tool="antismash"
                     /translation="LSPEKVFWPNEISLDILRRCDGQSSVERIIADLAADYDADPQDVA
                     ADVVAFLQEWS"
     aSDomain        10891..11049
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_MJ8_RS22690_Stand_Alone_Lasso_RRE.1"
                     /evalue="3.90E-09"
                     /identifier="RREFam002.1"
                     /label="MJ8_RS22690"
                     /locus_tag="MJ8_RS22690"
                     /protein_end="98"
                     /protein_start="45"
                     /score="27.4"
                     /tool="antismash"
                     /translation="NEISLDILRRCDGQSSVERIIADLAADYDADPQDVAADVVAFLQE
                     WSDKLLVK"
     gene            11052..12194
                     /gene="pqqE"
                     /locus_tag="MJ8_RS22695"
                     /old_locus_tag="MJ8_45200"
     CDS             11052..12194
                     /GO_process="GO:0018149 - peptide cross-linking [Evidence
                     IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532078.1"
                     /locus_tag="MJ8_RS22695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45200"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="WP_201410948.1"
                     /sec_met_domain="PTHR43273 (E-value: 7.1e-30, bitscore:
                     102.7, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.6e-24, bitscore: 85.7,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 2.9e-05, bitscore: 22.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 6.1e-10, bitscore:
                     37.9, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 1.4e-07, bitscore: 30.5,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEPLLPPIGMLAELTHRCPLQCPYCSNPVELLKANRELDTQTWL
                     DLFSQAADLGVLQVHLSGGEPTLRRDLEQLIAGLSARGVYTNLITAGVGIAEGRIEAFA
                     EAGLDHLQLSFQGARPATTDRIGNHRGSHEKKLETARRARAAGLPLTINAPVHRHNIEE
                     VPEFIDLALSLDAERLEIANVQYAGWALANRDRLMPDLAAVNRQADIVAAARERLVGIM
                     TIDFVTPDYFAIYPKPCMGGWARDAFMVAPDGTILPCHAAQTIPSLRFERFGDRRLAEI
                     WTDSPAFNVFRGTDWMHEPCRSCERREIDWGGCRCQAMAIAGDATATDPACVKSPIHNR
                     MAILTDAAQAEAASEGNESFIFRRIGAEIRPDTRAEPAHR"
     gene            complement(12247..13095)
                     /locus_tag="MJ8_RS22700"
                     /old_locus_tag="MJ8_45210"
     CDS             complement(12247..13095)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893847.1"
                     /locus_tag="MJ8_RS22700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45210"
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="WP_201410949.1"
                     /transl_table=11
                     /translation="MAKPLLDMSAARIFFDGIFSNPRVAHPEGVAVHRDGSIWCGTETG
                     DLLRLSADGGSVERMGCTDGFLLGIAFDGAGNCFACDLKHAAIFRWDAATGHMERFASS
                     GIRVPNYPVIDEARGRLYVSDSVGEEKRSGIFRYDLRTGEGGLWCREAMSFANGMAMAP
                     DANGLYVVESDAPCISYVPILTDGTAGRRQVVVEDVRNVPDGLAFASDGSLLISCYEPS
                     RIYRWRKDSGLEVLIEDPAATTLAHPTNIAFKGDKLYTANLGRWHITEIDLAGIPGLSG
                     A"
     gene            complement(13106..14893)
                     /locus_tag="MJ8_RS22705"
                     /old_locus_tag="MJ8_45220"
     CDS             complement(13106..14893)
                     /EC_number="2.8.3.-"
                     /GO_function="GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893846.1"
                     /locus_tag="MJ8_RS22705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45220"
                     /product="CoA-transferase"
                     /protein_id="WP_201410950.1"
                     /transl_table=11
                     /translation="MTDHRSVSLVKPNRPRFTDIEGLAAMVRDGDAFGVGGHHFARLPI
                     ALLRAIAGSGVKDLRYVSWAGGLPLELLLGAGAVAEIELCFSSLDILGLPPRFRAAAEN
                     GTIPVRDWTALAMIQALRAAQQNLPSMPFQLPEGSDMLARMPTAAARPDPLAGTDTGFV
                     PPLRLDTFIVHAQRADESGNVEIIGPRALDFAMAGAARKVLVTVEEIVPVGALRRDGRQ
                     SVLTRNQVSAIALAPGGAYPASCLPFYVTDYQALSEAFATKDAALWDKLSLPSGGLPER
                     LKKAAKVSAGTVLAMPALVQGEREASVDEIIAARIALELDNESFASAGAVSPLANVAYR
                     LAKAAHAPDMIIATMSCGHLDIAPSPMVLSLIESLDCETAVAHAGGDDTYSTYYQAGAV
                     THEIVGAAQVDRHGRVNTIALRKPSGGLIRLPGQGGMADVANMHRDYLLYIPRHSVQSL
                     VNEVETVSSARGLLTPAERKPMGYRTGKALVFTDLCVLRLVEASRELIVIETMPGVTRQ
                     QIRDATGFEVTFGADCREVPVPSSDTLALLRNRIDPLGLRRLEFVSAKERGALIAEILA
                     ADRAMVERCVAAQRLPAAS"
     gene            complement(14890..15903)
                     /locus_tag="MJ8_RS22710"
                     /old_locus_tag="MJ8_45230"
     CDS             complement(14890..15903)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893845.1"
                     /locus_tag="MJ8_RS22710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45230"
                     /product="hypothetical protein"
                     /protein_id="WP_201410951.1"
                     /transl_table=11
                     /translation="MRDDDYRGLGWAHGALTVQRLGAMLAPVTFVLSDGRQVSPMHVAP
                     WANEPESEALPGILRKLRGEWPCVPFGYSVPAEGWPESWARVMRPPAPDEEVHGHSSNH
                     DWAWRASGSGSLSLALAYPNASPVERVERTIRPDPSAPAIDIEFRIFVRRACRLPIGLH
                     PVFRLPFETGAATLELDGFDEGRTYPHDVEPGGSLFAGNKTFFELISVPARTGGTIDAS
                     RLPLAAATEELLQIEGVDGTAALVNHEDGYRVQLSWQKEHFPSLLLWYSNRGRKAAPWN
                     GRHVAIGIEPICSPFGLGPATALADNPIAQSGIATALEFSPGKPFVTRYRIEASAL"
     gene            complement(15900..16685)
                     /locus_tag="MJ8_RS22715"
                     /old_locus_tag="MJ8_45240"
     CDS             complement(15900..16685)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: ECH (9-252). E-value: 9e-63. Score:
                     203.8. Matches aSDomain: nrpspksdomains_MJ8_RS22715_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877395.1"
                     /locus_tag="MJ8_RS22715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45240"
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="WP_225247997.1"
                     /transl_table=11
                     /translation="MMGAYETLTVEIQGRVAVVTFRRADQLNAMNRQMQGEITEAFEAL
                     SGDENVGAIVVTGEGRGFMAGADIKEYAAQTSAEFDTFQAAGTRMYAAIEDNRKPVIAA
                     VNGFALGGGMELVLCCDIVIANQYAKLGLPEIKLGLIPGGGGTQRSVAKLGRNRANLLL
                     MTGAIVPASEFVDAGLVNEIVDVERLMPRALELAAMIAAEPAAAIEGLKALTAHAVSGG
                     LADGLAMERNILGGLYRSETGQRRVKEFAERSAVKKAGS"
     aSDomain        complement(15930..16658)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MJ8_RS22715_ECH.1"
                     /evalue="9.00E-63"
                     /label="MJ8_RS22715_ECH.1"
                     /locus_tag="MJ8_RS22715"
                     /protein_end="252"
                     /protein_start="9"
                     /score="203.8"
                     /tool="antismash"
                     /translation="VEIQGRVAVVTFRRADQLNAMNRQMQGEITEAFEALSGDENVGAI
                     VVTGEGRGFMAGADIKEYAAQTSAEFDTFQAAGTRMYAAIEDNRKPVIAAVNGFALGGG
                     MELVLCCDIVIANQYAKLGLPEIKLGLIPGGGGTQRSVAKLGRNRANLLLMTGAIVPAS
                     EFVDAGLVNEIVDVERLMPRALELAAMIAAEPAAAIEGLKALTAHAVSGGLADGLAMER
                     NILGGLYRSETGQRRVKEFAE"
     gene            complement(16685..17890)
                     /locus_tag="MJ8_RS22720"
                     /old_locus_tag="MJ8_45250"
     CDS             complement(16685..17890)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1189: L-carnitine dehydratase/bile acid-inducible"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877396.1"
                     /locus_tag="MJ8_RS22720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45250"
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="WP_140756038.1"
                     /transl_table=11
                     /translation="MNKALEGVRILDFSHLLQGPFATQLLGDMGADVIKIERAGAGDMF
                     RGMTFFAKWVGGSESPNFLAWNRNKRSIALNLKSRKVHAIIMEMARTADVVVQNFRPGV
                     LAKLGYGYEDFKAVNPRIIYCSGSGYGEEGPYVERPGQDMLIQGLAGLIANTGRGDQAP
                     VPAGAGLADQIGAMNMVYGILSALYYREKTGKGQKIEVNLLAGLLAHLNQEYVAVLNLG
                     EDFVRPNSGIGHPGMQAPFGIYETKDGGYVSIAMSPFKTLYEVLEAPQLAAYDDLKTLF
                     DKRDEVFEKVNAETRKFATQDLLARLLKADIWCAEVKDIRRAAEDPQVTHMGMITSYEH
                     PRGGKVRVVAPAVKMSETPATIERPAPLVGQHGREILAEFGLSKDEIAALEASGDMTVD
                     AA"
     gene            complement(17909..18694)
                     /locus_tag="MJ8_RS22725"
                     /old_locus_tag="MJ8_45260"
     CDS             complement(17909..18694)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (7-169). E-value: 1.4e-11. Score:
                     36.5. Matches aSDomain:
                     nrpspksdomains_MJ8_RS22725_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877397.1"
                     /locus_tag="MJ8_RS22725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45260"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_201410952.1"
                     /sec_met_domain="adh_short (E-value: 8.8e-36, bitscore:
                     122.1, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.5e-39, bitscore:
                     135.0, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARLAGKTAVITGAADGIGHAIAEAMAREGAHVFLGDIADDAGET
                     FAAALRAAGCRADYVHCDVSQEADIAGLIDSAVNKTGHLDILVNNAAIAIGGMPVHEMT
                     DEQWHRLIAVNLTSVFRGCKYALPHMIAQKSGSIINMASAQGHIGLDGWTAYAGAKGAV
                     MAMTRQMAVEFGPLNIRVNSISPGTINTPMNDRLIKEIGGSLAKAWVKMHPLGRIGEPS
                     EVAEAAVYLASDAAGFTTGTDLRVDGGLTAAPRYVPELL"
     aSDomain        complement(18188..18673)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MJ8_RS22725_PKS_KR.1"
                     /evalue="1.40E-11"
                     /label="MJ8_RS22725_PKS_KR.1"
                     /locus_tag="MJ8_RS22725"
                     /protein_end="169"
                     /protein_start="7"
                     /score="36.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TAVITGAADGIGHAIAEAMAREGAHVFLGDIADDAGETFAAALRA
                     AGCRADYVHCDVSQEADIAGLIDSAVNKTGHLDILVNNAAIAIGGMPVHEMTDEQWHRL
                     IAVNLTSVFRGCKYALPHMIAQKSGSIINMASAQGHIGLDGWTAYAGAKGAVMAMTRQ"
     CDS_motif       complement(18602..18667)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MJ8_RS22725_0001"
                     /evalue="1.20E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="MJ8_RS22725"
                     /protein_end="31"
                     /protein_start="9"
                     /score="14.7"
                     /tool="antismash"
                     /translation="VITGAADGIGHAIAEAMAREGA"
     gene            complement(18696..19691)
                     /locus_tag="MJ8_RS22730"
                     /old_locus_tag="MJ8_45270"
     CDS             complement(18696..19691)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1113:
                     inner-membrane translocator"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893841.1"
                     /locus_tag="MJ8_RS22730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45270"
                     /product="ABC transporter permease"
                     /protein_id="WP_201410953.1"
                     /transl_table=11
                     /translation="MSLASTASAKDQPSLLARLFARQELALLVCLVLVLAFFSATVENF
                     FSVRNMTNVLGQASLALTAGIGCAIVFISGEVDISIGSLLATIALPLIIIMNDTNSLLL
                     GVGGALLFGLIIGVVNGYLVAYAGINSLIVTLGTMFIMRGGVYLYTGQRAIPDDASLDS
                     FIALSDGRLFNIIPYSAVIAIVLLVAFGYMMQHRPFGRQIYAVGGNQEVARLAGYDVRR
                     VKFICFIISALLATVAGIILAARVGSAQHTAGLNFEFQVVAATVLGGVSLAGGIGSLTG
                     MALGVLILQFLSNGLRGLGLPTEWQLVITGMIIIAAVAFDEAKRRRANGG"
     gene            complement(19755..21287)
                     /locus_tag="MJ8_RS22735"
                     /old_locus_tag="MJ8_45280"
     CDS             complement(19755..21287)
                     /EC_number="7.5.2.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893840.1"
                     /locus_tag="MJ8_RS22735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_45280"
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="WP_201410954.1"
                     /transl_table=11
                     /translation="MSVDFLHPGEGAPIDTNVSALEMRGIGKRFPGVVALDNVDFTLEP
                     GKIHALMGENGAGKSTLIKIMAGVYGKDAGTIRMRGREVDIKSPRDSLKEGIKVVFQEI
                     ALISEFTVAENIFLEGYPTGRTGSIDWKKIRADSAALFNRIGFDVDPAARTGSLPVSQQ
                     QMVEIARALAHEARVVVMDEPTSSLTPNEVALLFTVIRRLAALGIAVVYVSHKLDEVFE
                     IADTVTVLRDGRHISTKPIGEHTNDTLIQDMIGRRIDNLFPRQRGAAGSKVALSVENLS
                     TAKKLIDVSFEARAGEVLGFFGLMGAGRTELAKAIVGYDPITSGIITVDGQRLAPHDTR
                     TGVRLGIGLLTEDRKSEGLMGELPVYQNASLASLGAFARLGFVDKAKEHQAVQDYVDRF
                     RIKTPSLFQQIKNLSGGNQQKVLISRWLMRGLKVIVVDEPTRGIDVGAKSEIFALIDRL
                     AGEGLAVVMMTSEMPELLGLADRIAVMCEGRLTALMPREEATQEKILNAAIG"
ORIGIN
        1 accttttcgc acgacctcga cggcggatat gtaaggtgct tcacccgcgc cgcgttccgg
       61 gtgccctcgc ctgttgcgtc cgtgctgctt catccgcttc ggcgcgtgga tacagcttgc
      121 cgagtagggg caggtaagtc gcacctttgc gcatcaggaa atcgttgaag gtcgccatgg
      181 ccggcgacat cacgtgatcg gttcgcatca ccgaaaacca ttgacggcgg atcggcatgc
      241 cggccacgtc gaggatgacg agccggccgg ttcccgtttc cgctgcgatc gtgcgggcgg
      301 agacttcagg tcgaaccgca cgaagcgctg atggttggag acagcatcag cgatgtcggc
      361 gcggcgcgcg cggccagcat gccgatcgct ctgctgcgcg gtggctacac ccatgtgccc
      421 gtagaagagc tggacgcgga tcttgtctgc gacagcctgc tcgatctgcc ctccgccatg
      481 cagaggctgc aagccgcggc ctgacaggcg gtgtcgatta cccagccagt cgcctaaact
      541 agccgatcga aaaagcgaac caccggcgaa gccgtcactt cgagagctgc ctcgacttga
      601 gaactgccct gctttgagaa ttgcccctgc tttgagaact gccctacttt gagaactgct
      661 tgagataggc gatgacgttg gccacgtcct tgtcgtcctt gaggccgaca aacgccatct
      721 tcgtgccctt aaccatggcc tttggatcgt ggagataggt cgtcagcgtc ggttcgtccc
      781 atttgacgcc ggactttcca gccgcgacca tggcctggga atagctgaaa cccggatgcg
      841 tgccggccgt gcggccgatg acgccgttca acgacggacc gaccttgttc tggtccttgt
      901 ccgcgacgtg gcagaccttg catttggtga agaccttctc accggccgcc gcgtcctgcg
      961 cataggattc gtccgtgatg aaagtaacca ggaaaatggc ggcgaaaaat gcgataacgc
     1021 gcatggcaat tcctcattaa tcgtcaaatg cccggcgccc gcaaaaagct aaccggaggc
     1081 aggcggaagt caatcgtacg aaggtccagt ctccggcatt cgaccgagtc gatcctgtac
     1141 tttcgccttt gttgacggtc ccgggacgag cgcgtagcct cggatcgttg ttggatttcc
     1201 aagggcacct cgggacatcg gtcaggctgg ctcgatccag gctgagattt gcccaggcaa
     1261 tggggtaggc ggcatttcgc tggagcgggc gtcgtcgcgc gcgagaggtg ctgaatgaac
     1321 atggtcgacc tcatcttgac ggtttgcctg agtgccaatc ccggcgcttg ccgggacgag
     1381 catctctatt ttgaaagccg cggctccctg ttccaatgca tgatgctggc gcccagcgaa
     1441 atcgcgaaat ggtcgcagga acacccggcc ttgaaggtca ggcgttggaa atgcgctttc
     1501 ccgacgaagg accgggcgat ctgaccggcg ccgctggttt gttccaaccc cgggagaacg
     1561 catgatgatt tcgcggcggg aaacgcttcg tttggcggct cttggaacgg cggcggttct
     1621 cggcgcacag tccattgccg ccgccgcgtc caaggtccgc atcggcgtgc tgaagttcgg
     1681 cacggtaagc tgggagcttg atacgctgaa gcaacatagt ttcgacgcgg ccaacgggat
     1741 cgatttggac gtcgtcaatt tcgccggcga ggatgcgacc aacgtggcga tgctggcagg
     1801 cgcgatcgac atcatcgtga ccgattggct gtgggtgtcg cgccagcgtt cggagggcgg
     1861 cgacgtgaca ttggccccct attcgacttc cgtcggcgcg atcatggtgg aggacgcatc
     1921 gccgatccgc acgatcgccg acctcaaggg aaagaagatc ggcgtcgccg gcggggcgct
     1981 cgacaagagc tggctgctga tacaggcgct ggccaggcgc gatcacggcc tcgacctgcc
     2041 ggcggtgacc gacgtcgtgt ttggcgcgcc gccgctaatt tcgcaaaagg ccatccaggg
     2101 cgaactcgat gcggcgctca acttctggca tttctgcgcc cggctcgagg ccggcggctt
     2161 tcgccggctg atcggcgccg acggcgcgca gatagcgctc ggcgcctcgg gcccggtatc
     2221 cgcgcttggc tatgtattcc acgacaaatg ggcgaacgag aacccgcggg ccgcccgcgg
     2281 cttcctcaag gcttccgcgc aggccaagga tctgctggca aggtccgacg aagagtggct
     2341 gcgcctcgcg ccggtcatcc gcgcggacgg caaggagttg gcaaagctgc gcgaccgcta
     2401 ccgcgagggt atccccaggc ggccggtcgc cgaggaagcg gccgatgccg gcaagctcta
     2461 tcgggtgctg gccgcaatcg gcggcgaaaa gctggtcggc agcgcgccgg agatggcccc
     2521 tggcacattc tggcgggagc cgccgcaatg acggtaaaag atggcggcgg gcacacgctt
     2581 ggcgaagcgg ccggcatttc caaaggttcc gtctcggcgg caatgctgat gcccgcgctg
     2641 acggtgacga tctccctgct cgggctcgga ctgctgtgga gcctggcggc gaatgcctgg
     2701 ccgagccgcg cctttccggg accgggacag gtctggcagg tattgctgag agaggcggcg
     2761 agcggcgacc ttttctacca cctcggcgcg accttcggcc gagtcgcggc cgcatacctc
     2821 gtcgcgatga tcgtcggatc ggtaatcggc atcctcctcg gcagctaccg ccgcgcggac
     2881 cggttcttca acccctgggt catcctgttc ctcaatattc ccgcgctggt gatcatcgtg
     2941 ctggcctata tctggttcgg cctcaacgag gcggcggcga tcggcgcggt tgccgttaac
     3001 aagatcccga atgtcgtggt gacaatgcgc gaaggcgcta gggcgctgga cccccgttac
     3061 gccgaaatgg cggcggtcta ccgcttcggt ccgcttgacc gccttcgcca catcctgctg
     3121 ccgcaactgc agccttatct ggcggcggcc tcgcgctccg gcatcgctct catctggaag
     3181 atcgtcctgg tggtggaact gctcggccgc tccaatggcg ttggcttcca gatccatctc
     3241 tatttccagc ttttcgacgt cgccgccatt cttgcctaca cgctggcatt cgtggcggtg
     3301 atgctggtga tcgaactact tctggtgcag cccgttgaac gacatgcaac ccgttggcgc
     3361 cgccgccccg cttagggtcg acatcgccga gaagaccttc agatccgcgc acggcgtctc
     3421 ggtcacagcg ctcaagaacc tttccttcga ggtccggcaa ggcgaattcg tctgcctgct
     3481 cgggccatcg ggctgcggca agaccacgac gctgcgcatt ctgctcgggc tcgacagaca
     3541 attctccggt tctttccagt tgcccggagg tggctcgaac cgcatagccg ccgtgttcca
     3601 ggaacccacc ctgctgccct ggcgaacggt ggaagagaat gtcaggcttg cgctgccgaa
     3661 aagtctgcgc atgaaaaatc tcgacgcgct gttagacacg ctcggcctca ccggcatgcg
     3721 ctccctctat ccgtccgagc tatcgctcgg ccttgcaaga agagtagccc ttgcacgggc
     3781 cttcgcgatc gaacctgcgg tgctgttcct cgacgagccg ttcgtctcgc tcgacgaacg
     3841 gaccgccggg cagctccggc gcctgcttct tgatgtctgg tcggcccggc ccacgacagc
     3901 cctgatggtg acgcacaacc tgaccgaagc cctggcgctt tcggatcgca tcatcgtgct
     3961 tgcgccgcgg ccctcgcacg tgctcggcgt ttttgacata ggactgccca ggcagcaccg
     4021 cagccccgag gcggcgaacg accttttgcg atccttccat cagaaatttc cgggcgtgac
     4081 ctgaccgcct ggaaaccgct tccgtgtgac ggcgaacctc tctagaagca acgaacgtcc
     4141 gcttccgcct gcgcctgctt cagctccgtt cgcgcggctt tcgccggcgc gctttcgcgg
     4201 aacagacctg cattctcgcc cgttgtcaga gacatgctct tgaaggtctc ggccctctca
     4261 taatggtcgt aaggcaggat cgaggcgatg acatcgatcg agcatgaaca actctccagc
     4321 gcttggcgtg tctggccgtt tgtgcgcatg cagccgagca cgtagtcgac gatcgtcgcg
     4381 gttggataat ctcctgcggc gccagcaggg tttgcaaaga ccgttgccga cgagaagaga
     4441 agcgtcagga tcgaaaatcc agcatatctg accattggaa attcctccta ttgcccgccc
     4501 gcgccgcacc gaggcggcac catcgtgccc agttggcggc acgccgcaag cccggtcaag
     4561 ctcccaccgc cccggtatag gaccaaagga ggaatgcttt gcccacggac agcagcgctc
     4621 caaggcagct tcacggccca cctcagaacc aaccttcacg agtacctttt acccccagat
     4681 caatatgaaa cgccaaaaaa atccagcgat aaaagacgaa ataatattca aaaacataat
     4741 tcaaagttat tacaatcata aatgcacaaa tataggattc tatatatatg actaacaaac
     4801 tttgcactgg cacatggctt gaagtttcca atatctttta tctgccaatt ttatattgcg
     4861 ccgcatcaaa tgtatgttgc gatgcacaat cccaacaggg ctttttcctt gcctgacttt
     4921 ttgcaatgac atagccttac cctgtttccg gcttcaagga cttcgccgta acaagggagc
     4981 ggcgggtgtc tttcgtaggc caaccgggcg ggtgcaggct aggctgactt ggtagagggc
     5041 agaaacgttc ctctgctatt tgcagcttag gtcgtgacag ctgccggaaa tacttcccag
     5101 gatgaatcgc ttggacaagc tcttgcgagc ttgaatacga tttcttgttg tcaactcggg
     5161 aggaatttga tgcgcgcact tacgcaagct acctacatcg caactaccac tgcgctactg
     5221 tcgttgggcg cgctctatac agcgtccgcc aacgaagaac tcgcgaagat ggccgaaaac
     5281 ccgaaagact gggtgatgca gacgggtgat tacgccaaca cccgttactc cacactgaag
     5341 caaatcacca aggacaatgt gaagaacctg caggtcaagt ggaccttctc gaccggcgtg
     5401 ctgcgcggcc atgagggcgg gccgctgatc atcggcgacg tgatgtatgt tcacgccccg
     5461 ttcccgaaca ccgtctatgc gctcgatctc aacaaggatg gaaagatcct gtggaagtac
     5521 gagcccaagc aggacaccaa cgtcattccg atcatgtgct gcgacacggt caatcgcggc
     5581 gttgcctatg cgcctgccga tggcgaccgc ccggcgctgg tcatcctcaa ccaggccgac
     5641 actacggtcg tggcgctcga tgccaagacc ggaaaggtcg tgtggtcggt caagaacggc
     5701 gagaacaccg acggcggaaa gggcgaatcc ggcacctcgg cgcccgtggt cgtcaaggac
     5761 aaggtcctcg tcggcgtctc cggcgcggag ttcggcgtcc gcggctggct ggccgcctac
     5821 gatctgaagg acggcaagct cgcctggaag gcttattcgg aaggtccgga cgcggacacg
     5881 ctgctcgatc ccgagaagac gacgcagctc ggcaagccgg tggggaagga ctccggcacg
     5941 aacacctggg aaggcgagca gtggaagaca ggcggcggca cgacatgggg atggtattcc
     6001 tatgatccca agctgaacct cgtctattac ggcaccggca atccctcgac ctggaatccc
     6061 gtccaacgtc ccggcgacaa tcgctggtcg atgaccatct tcgcccgcga tgccgacacc
     6121 ggcgtggcca agtgggtcta tcagatgacc ccgcatgacg aatgggacta tgacggcatc
     6181 aacgagatga tcctcgtcga cggcatggag gtcaaaggca agaagcacga cgtgctggtg
     6241 catttcgacc gtaacggctt cgcctacacc atggatcgcg ccaccggcga gcttctcgtc
     6301 gccaagaagt acgatccggc ggtgaattgg gcgaccgaag tcaatatgga ccccaacagc
     6361 aaggaatacg gccgcccgca ggtcgtggcc aaatattcca cgcagcagaa cggcgaagac
     6421 acaaactcga cgggtatctg cccggcagcg cttggaacca aggaccagca gccggccacc
     6481 tactcgccga agaccgggct attctatgtg ccgaccaacc acgtctgcat ggactatgag
     6541 ccgtacaagg tgagctacac cgccggccag ccttatgtgg gcgccaccgt atcgatgtat
     6601 ccggcgcccg gcggcgacgg caacatgggc aacttcattg cctgggatgc cgccaagggt
     6661 gagatcgtct ggtcgaagcc cgagcagttc tcggtgtggt cgggcgcgct ggcgaccgcc
     6721 ggcgacgtca tcttctacgg gacgctcgaa ggctacatca aggcggtcga tgagcacggc
     6781 aaggaactct acaagttcaa gaccccatcc ggcatcatcg gcaacgtcac gcccttcgag
     6841 cacaacggca agcaatatat cgccgttctg tccggtgtcg gcggctgggc aggcatcggc
     6901 ctcgccggcg gactgctgtc acccgacaac gccgctgcct ggcacggcgc cgtcgatcag
     6961 ggccgcgcgc agggcgatca ggccgcggtc gtcggaaccg ccggtctcgg agcggtcggt
     7021 ggctacgcgg cgcttgccga ctacacgacg ctgggcggcc agctcaccgt cttcggtctt
     7081 ccggattgat ctcccggaaa aaccttaaga ccgaaggcag aaagatcgag agcccggatc
     7141 atccccaggc aacggccggg ctctcgatct gaagcacacc cgaacaccgt caacgcccgc
     7201 tcgcgacggc aacccctcgt cctttggcaa aacaaggaag tgtctgaatg ttcatccgca
     7261 tcgcagtttc cgttctcgcc ctgacccttc tgcccttggc cggctcgatt tcggcgcagg
     7321 ccgaagacag cgccgacaaa gccaaggcgg tcaaggacga gggcggcaaa tatttggatg
     7381 cggatggaaa tccaacctac aagatcgaga acggaaccgt ggattggtac acgttcagcg
     7441 gctatcgccg ctaccactcg gactgccatg tctgccacgg gcaggacggg atgggttcga
     7501 cctatgcccc ggccctggcg aacagcctta aaacgatcga ctaccccaca ttcctctcga
     7561 tcgtcgccca gggccgcaag aacaccgcca acggcaagga gaatgtcatg cctgcctttg
     7621 gcgacaacaa gaacgtctac tgctatctgg atgacctcta cgtctatctg cgcgcccgcg
     7681 ccgacggcgc cgcgccgcgc ggcaggccgc ccaagcacga ggacaaaccc caggcggcca
     7741 aggatgccga aaaatcctgc atgggaggat gacgtgaccg gccgggttgc atcgaagccg
     7801 cgtttcgttc tgcctctgct cggcgcgctc gccttgctgc catcggccgc ctgggcgcaa
     7861 ggcgccggtc ttggcgccgc gggcgaactg gtcgaccccg acattctgcg cgtctgcgcc
     7921 gatccttcga acatgccttt caccgaccag aacggccagg gattcgaaaa caagctggcc
     7981 gagttggtcg ccaggaagac aggacggaag tcggtcgcct acacctggtt tccgaccgtg
     8041 atcggcttcg tgcgcaacac gcttgccgcc aaccgatgcg acgtcatcat gggctatgcc
     8101 cagggcgacg agctggtgca gaacaccaac gcctattatc gctcctccta tgtgcttgtg
     8161 catcccaagg gcgacggcct cgaaggtgtc gagacgatcg aggatccgaa actcgccggc
     8221 aagcggatcg gcgtcgtcga acgcacgccg ccgaccgcca acatggccgc caacaagctc
     8281 ttgcggacgg caaagatcta cccgctggcc gtcgacacgc gcgtcacccc gtcgatggga
     8341 gaggtcatga tcaaggacat gctcgccggc aagatcgacg cggcgatcct gtggggaccg
     8401 atggccggtt actatgccaa gcagctcgac gcgaatgtcg cgatcgtccc gctggtcaag
     8461 gaaaagacag gttcgcgcat gtcctaccgc atcaccatgg gagtgcgccc atccgaccag
     8521 gaatggaagc gtaccctcaa caaggtgatc cgcgagaacc aggcggagat caacaagctc
     8581 ctgctcgact acaacgtgcc gctgatcgac gaacatgaca acccgatcac ggcgacgccg
     8641 gggtgagcgc gatggagcgg aagcagcaga ggcgatggag gtaaggaacc gatcttcgat
     8701 acggacagtt tcttttagtt gtttgaggac ccggtgtttc atttgtccat ttgtgaagtg
     8761 atggacgagc cattgcaccc gcccgcaatg gcgccaacga catgaccggc cccctcgggc
     8821 tggcagaaga ggagaatgcg atgaagaaga gctggaagaa accgactatg tgccaggttg
     8881 ctgcgggctt cgaaatttcg cggtatctgc ctgccgaaat tcctcccagg aagtaggccg
     8941 caagggtgca tgtccctggc agcaggggca tgcaccccgc cgcatgtgag ggcattgcag
     9001 cgcaaaacac ggatacgaaa ctctctgccg ccgacaagcc ggtcggatgg gccgcggagc
     9061 gggcatttgc catgcgcgtg aagatcgtcg gatcggcggc gggaggcggc tttccgcagt
     9121 ggaactgcaa ctaccgcctg agccgcgcgg cccgcgccgg cgtgcccggc atgcgtccac
     9181 gaacccaatc gtccatcgca gtatcggcgg acgggacagg ttgggttctc ttcaacgcgt
     9241 cgcccgacat ccgccagcag attgcgcaag cgcccgaact gcagcccgcg tccgacgcac
     9301 cgcggcgctc gacgccgatc cgcgcggtgg tgctgaccaa tgccgacgtg gaccatgtcg
     9361 cgggcctgct cagcctgcgt gaaagacagc cattcgcgat ctatgcgagc gcgcaggtgc
     9421 tggcgacact ggaggccaac tcgatcttca atgttctcga tccggcgatc gtaccccggc
     9481 gcctgctggc gccgacggag gagatgacga tccgcgatgc ggcgggccgg gagaccggcg
     9541 tcgtgatcga gagctttccg gtgccgggca aggttgcgct ctatctggag gacaaagacc
     9601 atccggatgc cgatttcagt tccgatgccg gcgacaccgt cgggatccgc atcgccggct
     9661 ccggcggcgc ggtcttctac ataccgggct gcgcgcgcat cgatgccgcg ctgcgcacgc
     9721 gcctggccgg cgccgcctgc cttctgttcg acggcaccgt ctacacggac ggcgagatga
     9781 tcgcggccgg cgtcggccat aaaaccggcg cgcgcatggg gcacatcgcg atgtcgggac
     9841 aagcaggttc gatcgccggc ctcgccgatg tgaagatcgg tcgccgtatc ttcatccata
     9901 tcaacaacac caatcctgtt ctggacgaga attccgccga gcacgcggcg gtcaaggcgg
     9961 ctggatggga agtcgccagc gatggcatgg agatggaatt ttgagcgatt tccacgacgt
    10021 ggcccgagac ggattgtcga agcgcgatct cgaggccgtg ctgcggcggg tcggcgacga
    10081 gcgctatcac aaccgccatc ctttccatca caggatgacc ggcggtgcgc tgtcgaaggc
    10141 cgagatgcag gcctgggcgc tgaaccgcta ttgctaccag gccgtcattc cacgcaagga
    10201 cgccatgatc ctcgcacgtg ccgaagaccc ggcgttccgc gccgcctggc gcaagcgcat
    10261 cgaggaccat gacggcgagg acggctggag cggcggcatc gcccgctggc tgcatctggc
    10321 gacctcgctc gggctcgatg ccgaagccgt caagagcgaa aggctggcgc tgccggcgac
    10381 tcgtttcgcc gtcggcgcct atctttcctt ctgcacgaat cggacgctgc tggaagcggt
    10441 cgcatcgtcg ctgaccgaga tgttctcgcc acggatcatc ggcgagcggg taccggcgat
    10501 gctggcgaaa tacgattaca tcaccgagga cacgctggcc tatttcacgc agcggccgga
    10561 gcaggcgaag cgtgacgccg atttcgccct cgcctatgtg ctggcccatg ccgacacggc
    10621 cgaacgccag cagcgggcga tcgatgccct ggtgttcaaa tgcgatatcc tctgggccat
    10681 gctcgatgcg ctccagcatg cctatggcac ccccggaaac ataccgcccg gagccttctg
    10741 cccggaggcg gccttatgat gacggcgctg cgcgaaagag ccatcgtgtc gtcgcgatcg
    10801 gtgccatcgc tgcccaggca tgtgcgcatc cagtatgatc ccgtgcggca ggccttcgcc
    10861 gtgctttcgc cggagaaggt gttctggccg aacgagatca gcctcgacat actgcgccgc
    10921 tgtgacggcc agtccagcgt cgagcgcatc attgccgatc ttgctgccga ctacgacgct
    10981 gatccgcagg acgtggcggc cgacgtcgtc gccttcctgc aggaatggtc agacaagctc
    11041 ttggtgaagc tatgagcgag cccctcctgc cccccatcgg catgctggcg gaactgacgc
    11101 atcgctgccc gctgcagtgc ccctattgct ccaacccggt cgaactgctg aaggccaacc
    11161 gcgagctcga cacgcagacc tggctcgatc tcttctcgca ggccgccgat ctcggtgtcc
    11221 tgcaggtgca tctttccggc ggcgagccga cgctgcgccg cgacctcgag caattgatcg
    11281 ccgggctctc ggcgcgcggt gtctacacca acctcatcac cgccggtgtc ggcattgccg
    11341 agggccgcat cgaggccttt gccgaagccg ggctcgacca cctgcaactg agcttccagg
    11401 gcgcccgtcc ggcaaccacc gaccgtatcg gcaaccaccg gggcagccat gagaagaagc
    11461 tggagacggc gcgccgcgcc cgcgcggccg ggcttcccct caccatcaat gcgccggtcc
    11521 atcggcacaa catcgaggaa gtgccggaat tcatcgatct cgccctctcc ttggatgccg
    11581 agcggctcga gatcgcgaac gtccagtatg ccggttgggc gctcgccaac cgcgacaggc
    11641 tgatgcccga cctggcagcg gtcaaccgcc aggcggacat cgtcgcggcg gcgcgcgaaa
    11701 ggctcgtcgg catcatgacc atcgatttcg tcacgccgga ttatttcgcc atctatccca
    11761 agccgtgcat gggcggctgg gcgcgcgacg ccttcatggt cgctcccgac ggcaccatat
    11821 tgccgtgcca tgccgcccag acgattccct cgctccgctt cgagcgcttc ggagaccgca
    11881 ggctcgccga aatctggacc gattcaccgg ccttcaacgt cttccgcggc accgactgga
    11941 tgcacgagcc gtgccgaagc tgcgagcgcc gcgagatcga ttggggcggc tgcaggtgtc
    12001 aggcgatggc gattgccggc gatgccaccg caacggatcc ggcctgcgtc aagtcgccga
    12061 tccacaatcg aatggcgata ctgaccgacg ctgcgcaggc ggaggccgcg tcggagggaa
    12121 atgaaagctt cattttccgg cggatcggcg ccgaaatccg acctgacacg cgcgccgagc
    12181 ctgcccaccg gtaacggccc gaaggatttc tcttccttgg cggtatcgcc ttgtgtctct
    12241 tgccgatcag gcgccgctca agcccggaat cccggccaga tcgatctcgg tgatgtgcca
    12301 gcggccaagg ttcgcggtgt agagcttgtc gcccttgaag gcgatgttgg tgggatgcgc
    12361 cagcgtcgtc gccgccggat cctcgatcag cacttcgagg ccgctgtcct ttcgccagcg
    12421 atagatgcgg ctcggctcgt agcatgagat gagcagcgag ccgtcggaag cgaaggcgag
    12481 cccgtccggg acattgcgca cgtcttcgac gacgacctgc cggcgtcccg ccgtaccatc
    12541 ggtgaggatc ggcacatagc tgatgcaggg cgcgtcgctc tcaaccacat agagcccgtt
    12601 ggcgtccggc gccatggcca tgccattggc gaaggacatc gcttcccggc accacaggcc
    12661 gccctcaccc gtccgcagat cgtagcggaa gatgcccgaa cgtttttcct cgcccacact
    12721 gtcagacacg tagagccggc ctcgcgcctc gtcgatgacc ggatagttcg gcacgcggat
    12781 gccggacgaa gcgaagcgct ccatatgacc cgtcgcggca tcccagcgga agatcgcggc
    12841 atgcttgaga tcgcaggcaa agcaattgcc ggcgccgtcg aaggcaatgc cgagaagaaa
    12901 gccgtcggta cagcccatgc gctcgaccga accgccatcg gcggaaagcc tcaagaggtc
    12961 gccggtctcg gtgccgcacc agatcgatcc gtcgcgatgg acagccacgc cctcgggatg
    13021 cgcgacgcgc ggattgctga agatgccgtc gaagaagatc cgggcggcgg acatatcgag
    13081 caacggcttt gccatggaac gctcctcagc ttgcggcagg taatctctgc gcggcgacgc
    13141 agcgctcgac catggcgcgg tcggccgcga ggatctcggc gatgagcgcg ccacgctcct
    13201 tggcgctgac gaattccaga cgccgcaagc caagcggatc gatgcggtta cgcaacaagg
    13261 caagcgtatc gctggagggc accggcacct cgcggcaatc ggcgccgaat gtcacttcga
    13321 acccggtggc gtcacggatc tgctgccggg tgacgccggg catggtttcg atgacgatca
    13381 attcccgtga tgcctcgacg aggcggagga cgcagaggtc ggtgaagacc agcgccttgc
    13441 ctgtcctata gcccatcggc ttacgttcgg ccggcgtcag cagaccgcgc gccgagctga
    13501 ccgtctcgac ctcgtttacc agggactgga ccgagtgacg tggtatgtaa agcaggtagt
    13561 cgcgatgcat gttggcgaca tcggccatgc cgccctgccc cggcagccgg atcaacccgc
    13621 cgctcggctt gcgcaacgcg atggtgttga cccggccgtg ccggtcgacc tgcgccgcgc
    13681 cgacgatctc atgcgtcacc gcgcccgcct ggtaataggt cgaataggtg tcgtcgccgc
    13741 cggcatgcgc gaccgccgtc tcgcaatcga ggctttcgat cagcgacagc accatcgggc
    13801 tcggcgcgat gtcgagatgg ccgcagctca tcgtcgcgat gatcatgtcc ggcgcgtgcg
    13861 ccgccttggc cagccggtag gcgacgttgg cgagcggcga gaccgcgccg gcgctggcga
    13921 agctctcatt gtcgagctcg agggcgatcc gcgcggcaat gatttcgtcg acggatgctt
    13981 cccgctcgcc ctgcaccaat gcaggcatcg cgaggacggt cccggcgctg actttggccg
    14041 ccttcttgag ccgttccggc aggccgcccg aaggcaggct tagcttgtcc cacaacgccg
    14101 cgtccttcgt cgcgaacgcc tccgacaatg cctgataatc cgtgacgtag aacggcaggc
    14161 atgaggccgg gtaggcgcca cccggtgcca gcgctatcgc cgaaacctgg ttgcgggtaa
    14221 gcacgctctg gcggccgtcc cgccgcaacg cgccgaccgg cacgatctcc tcgaccgtga
    14281 ccagcacttt ccgcgccgcg cccgccatcg cgaagtccag cgcccgcggt ccgatgatct
    14341 cgacattgcc gctttcgtcg gcgcgctggg cgtggacgat gaaggtgtcg agcctgagcg
    14401 gcgggacgaa ccccgtatcg gtcccggcca gcggatcggg cctggcggcc gcagtcggca
    14461 tgcgcgccag catgtccgat ccttcaggaa gctggaacgg catcgacggc aggttctgct
    14521 gcgctgcccg cagcgcctgg atcatggcca gtgccgtcca atcacggaca gggatcgtgc
    14581 cgttctccgc cgccgcgcga aacctcggcg gcagcccgag gatatccagg ctggaaaagc
    14641 acagctcgat ttccgccacc gcgcccgccc cgagcagcag ttcgagcggc aggccgccgg
    14701 cccacgagac atagcgcaaa tctttcacgc cggacccggc gatcgcgcgc agcaacgcga
    14761 tcggcagccg ggcgaagtgg tgaccgccga cgccgaaagc gtcgccgtcg cggaccatcg
    14821 ccgccagccc ctcgatatcg gtgaagcggg gccggttcgg cttgaccagg gaaactgacc
    14881 tgtgatctgt cacagcgccg atgcctcgat gcggtagcgg gtcacgaacg gtttcccggg
    14941 cgagaattcc agcgccgtcg cgatgccgga ttgcgcgatc ggattgtcgg cgagcgcggt
    15001 cgccgggccg agcccgaaag gcgagcagat cggctcgatg ccgatcgcga catgacgccc
    15061 gttccagggc gccgccttgc gtccgcgatt ggaataccag agcaggaggc tcgggaaatg
    15121 ctccttctgc caggatagct gcacccggta tccgtcctca tggttgacga gcgccgcggt
    15181 accgtcgacg ccctcgatct gcagcaactc ctccgtggcc gcagccaggg gcaaacggga
    15241 ggcgtcgatc gtgcccccgg tgcgcgccgg caccgatatc agctcgaaga aggtcttgtt
    15301 cccggcaaac agtgaaccgc cgggttcgac atcgtgtgga taggtgcggc cttcgtcgaa
    15361 gccatcaagc tcgagtgtgg cggcgcccgt ctcgaacggc agccggaaca ccgggtgcag
    15421 accgatcggc aggcggcagg cgcggcggac gaatatccgg aactcaatgt cgattgccgg
    15481 cgccgacgga tcgggcctta tcgtccgttc gacacgctcg accgggcttg cgttgggata
    15541 ggcaagcgcc agcgacagcg atccgctccc actggcgcgc caggcccagt catgattgga
    15601 gctgtggccg tggacttcct cgtccggagc cggcggccgc atgacgcgcg cccagctctc
    15661 gggccatcct tcggcaggca ccgaatagcc gaagggcacg cagggccatt cgccgcgcag
    15721 cttgcgcaga atgcccggta gcgcttccga ttccggttcg tttgcccagg gtgcgacatg
    15781 catcggcgag acctgccggc cgtcggaaag cacaaacgtg accggcgcga gcatcgcgcc
    15841 gagccgctgc acggtcagcg cgccatgcgc ccagccgagg ccacgatagt cgtcgtccct
    15901 catgagccgg ccttcttcac cgcgctcctc tcggcgaact ccttgacgcg ccgttggccg
    15961 gtctcgctgc gataaaggcc gcccagtatg ttgcgctcca tggcgaggcc atccgccaga
    16021 ccgcccgaca cggcatgcgc cgtcagggcc ttcaggcctt cgatcgcggc ggctggctcg
    16081 gcggcgatca tcgcggccag ttcgagtgcg cgcggcatca gcctttcgac atcgacgatc
    16141 tcgttgacca ggccggcatc gacgaattcg gacgccggca cgatggcgcc ggtcatcagc
    16201 agaagattgg cgcggttgcg cccgagcttg gcgacgctgc gctgcgtgcc gccaccgccg
    16261 gggatcagtc cgagcttgat ctcgggcagg ccgagcttgg catactggtt ggcgatcacg
    16321 atgtcgcagc acagcaccag ctccatgccg ccgcccagcg cgaagccgtt gaccgccgca
    16381 atcaccggct ttcggttgtc ctcgatcgcc gcgtacatgc gcgtgccggc ggcctggaac
    16441 gtgtcgaact cggccgaggt ctgcgcggca tattccttga tgtcggcgcc ggccatgaag
    16501 ccgcgcccct cgccggtcac cacgatcgca ccgacatttt cgtcgcccga cagcgcctcg
    16561 aacgcctccg tgatctcgcc ttgcatctgc cggttcatgg cgttgagctg atcggcgcgc
    16621 cggaaggtga ccaccgcgac gcgaccttgg atctcgaccg tgagtgtctc gtaggcgccc
    16681 atcatcaggc ggcgtccacg gtcatgtcgc cggacgcctc gagcgctgcg atctcgtcct
    16741 tcgacaggcc gaactcggcc aggatttcgc ggccatgctg accgaccagc ggcgccggcc
    16801 gctcgatggt cgcgggcgtc tcgctcatct tgacggcggg cgccaccacc ctcaccttgc
    16861 cgccgcgcgg atgctcgtag ctggtgatca tgcccatatg cgtgacctgc ggatcctccg
    16921 ccgcgcggcg gatgtccttc acctccgcgc accagatgtc ggctttgaga agccgcgcca
    16981 gcagatcctg cgtggcgaat ttcctggtct cggcattgac cttctcgaac acctcgtcgc
    17041 gcttgtcgaa caaggttttc aaatcgtcat aggcggcgag ctgcggcgct tccagaacct
    17101 cgtaaagcgt cttgaacggg ctcatggcga tcgacacgta gccgccgtcc ttcgtctcgt
    17161 agataccgaa cggcgcctgc atgccgggat ggccgatgcc ggaattgggc cgcacgaaat
    17221 cctcgccgag attgagcacg gcgacatatt cctggttgag atgcgccagc agcccggcca
    17281 gcagattgac ctcgatcttc tgacccttgc cggtcttctc gcgataatag agcgccgaca
    17341 ggatgccgta gaccatgttc atggcgccga tctggtcggc gaggccggcg ccggcgggaa
    17401 ccggtgcctg gtcgccgcgg cccgtattgg cgatcagccc ggccagccct tggatgagca
    17461 tgtcctgccc cggacgctcg acgtaaggcc cctcctcgcc atagccggaa ccggagcagt
    17521 agatgatgcg cggattgacg gccttgaaat cctcgtagcc gtagccgagc ttggccagca
    17581 cgccgggacg gaaattctgc accaccacgt cggcagttcg ggccatctcc atgatgatgg
    17641 catgcacctt gcggctcttc aggttgagcg cgatcgagcg cttgttgcgg ttccaggcga
    17701 ggaagttcgg gctctcggag ccgcccaccc atttggcgaa gaaggtcatg ccgcggaaca
    17761 tgtcgccggc gcccgcgcgc tcgatcttga tgacgtcggc gcccatgtcg ccgagcaact
    17821 gcgtggcgaa cggcccctgc agcagatggc tgaagtcgag gatgcggacg ccttcgaggg
    17881 ccttgttcat ttcatgtctc gtttcgtttc aaagcagttc gggaacgtag cggggcgccg
    17941 ccgtcagtcc gccatcgacg cgcaggtcgg tgccggtggt gaagccggcc gcatcggaag
    18001 ccagatagac ggcggcctcg gccacctccg aaggctcgcc gatgcggcca agcggatgca
    18061 tcttgaccca cgccttggcg aggctgccgc cgatttcctt gatcagcctg tcgttcatcg
    18121 gtgtgttgat ggtgcccggc gagatcgagt tgacgcggat gttcagcggg ccgaactcga
    18181 ccgccatctg gcgcgtcatc gccatcaccg cgcccttggc accggcatag gcggtccagc
    18241 cgtcgaggcc gatatggccc tgcgccgagg ccatgttgat gatcgagccg gacttctgcg
    18301 cgatcatgtg cggcaatgcg tatttgcagc cgcggaacac gctggtcagg ttgaccgcga
    18361 tcagccggtg ccattgctcg tcagtcatct cgtgcaccgg catgccgcct atcgcgatcg
    18421 cggcgttgtt gaccaggatg tcgagatgtc cggtcttgtt caccgcgctg tcgatcaggc
    18481 cggcgatgtc ggcttcctgg gagacgtcgc aatggacgta gtcggcacgg cagccagctg
    18541 cgcgtagcgc tgccgcgaac gtctcgcctg cgtcgtcggc gatgtcgccg aggaacacat
    18601 gcgccccttc ccgggccatc gcctcggcga tcgcgtggcc gatgccgtcg gccgcaccgg
    18661 tgatgacggc ggtttttcct gcaagacgag ccatatcagc ctccattcgc gcgccggcgc
    18721 ttcgcttcgt cgaaggcgac cgcggcaatg atgatcatgc cggtgatgac gagctgccat
    18781 tcggtcggca ggccgagacc gcgcaggccg ttggacaaga actgcaggat gagcacgccc
    18841 agcgccatgc ctgtcaggct gccgatgccg ccggcaaggc tcacgccgcc gagcaccgtc
    18901 gcggcgacga cctggaattc gaaattgagc cccgccgtgt gctgcgccga gccgacacgc
    18961 gccgccagga tgatgcccgc gacggtcgcc agcagtgcgc tgatgatgaa gcagatgaac
    19021 ttgacgcgcc gcacgtcgta gccggcgagc cgcgccactt cctgattacc gccgacggca
    19081 tagatctgcc gtccgaaagg cctgtgctgc atcatgtagc cgaaggcgac gaggagcacg
    19141 atggcgatca ccgccgaata cgggatgatg ttgaacaggc gcccgtcgga gagcgcgatg
    19201 aaggagtcga ggctggcatc gtcggggatc gcgcgctggc cggtatagag atagacgccg
    19261 ccgcgcatga tgaacatggt gcccagcgtc acaatcagcg agttgatgcc ggcataggcg
    19321 acgagataac cgttcaccac accgatgatc aggccgaaca gcagcgcgcc gccgacgccg
    19381 agcagcagcg aattggtgtc gttcatgatg atgatcagcg gcagcgcgat ggtcgccagc
    19441 agcgagccga tcgaaatgtc gacctcgccg gaaatgaaga cgatggcgca gccgatgccg
    19501 gcggtgagcg ccagcgaggc ctgccccagc acgttggtca tgttgcgcac ggagaagaaa
    19561 ttctcgaccg ttgcggaaaa gaacgccagc accaggacga ggcagacgag caacgccagt
    19621 tcctgccggg caaagagccg cgccagcagg gatggctggt ctttcgcgga agccgtgctt
    19681 gcaaggctca tcgcttgcct cctccgaggg aatggcgctt atcgcaagga atgccgaggc
    19741 gatgcgccat ggttctatcc gatcgccgca ttgaggatct tctcctgcgt ggcttcctcg
    19801 cgtggcatca gcgcggtcag gcggccctcg cacatcaccg cgatccgatc ggcgaggccg
    19861 agcagttccg gcatctccga cgtcatcatg accaccgcca agccctcgcc ggcgagccgg
    19921 tcgatcagcg cgaagatttc cgacttggcg ccgacatcga tgccgcgtgt cggctcgtcg
    19981 acgacgatga ccttcaggcc gcgcatcagc cagcgcgaga tcagcacctt ctgctggttg
    20041 ccgcccgaga ggttcttgat ctgctggaac aggctggggg tcttgatgcg gaagcggtcg
    20101 acatagtcct gcaccgcctg gtgttccttc gccttgtcga cgaaacccaa tcgggcgaac
    20161 gcgccgagcg aggcgaggct ggcattctgg tacacgggca gctcgcccat caggccctcg
    20221 cttttgcggt cctcggtgag caggcctatg ccgagccgca ccccggtgcg cgtgtcgtgc
    20281 ggcgcaaggc gctgaccgtc gaccgtgatg atgccagagg tgatcgggtc gtagccgacg
    20341 atcgccttgg cgagctcggt gcggccggcg cccatcaaac cgaagaagcc gagcacctcg
    20401 ccggcgcgtg cctcgaagga cacgtcgatg agcttcttgg ccgtgctgag gttttcaacc
    20461 gacagtgcca ccttgctgcc cgccgcgccg cgctggcgcg gaaagaggtt gtcgatccgg
    20521 cggccgatca tgtcctggat cagcgtgtcg ttggtgtgct cgccgatcgg cttggtcgag
    20581 atgtggcgtc catcgcgcag caccgtcacc gtgtccgcga tctcgaacac ctcgtcgagc
    20641 ttgtggctga catagaccac ggcaatgccg agcgccgcca ggcggcggat gacggtgaac
    20701 agcagcgcaa cttcgttcgg cgtcagcgag gacgtcggtt cgtccataac caccacgcgc
    20761 gcctcgtggg cgagcgcgcg ggcgatctcg accatctgct gctggctgac cggcagcgat
    20821 cccgtccgcg ccgctggatc gacgtcgaag ccgatgcggt tgaagagtgc cgccgagtcg
    20881 gcgcggatct tcttccagtc gatcgagccg gtcctgccgg tcggatagcc ttcgaggaag
    20941 atgttttccg cgacggtgaa ttcggagatc agcgcgatct cctggaacac gaccttgatg
    21001 ccttccttca ggctgtcgcg cggcgatttg atgtcgacct cccgcccgcg catgcggatg
    21061 gtgccggcgt ccttgccgta gacgccggcc atgatcttga tcagcgtcga cttgccggcg
    21121 ccgttctcgc ccatcagggc gtggatcttg ccgggctcca gggtgaagtc gacattgtcc
    21181 agggccacca cgcccgggaa gcgcttgccg atgccgcgca tctccagcgc cgacacattc
    21241 gtgtcgatcg gcgcgccttc gccgggatga aggaaatcaa cggacattgg ctttgttccg
    21301 gatgatgtcg agataggtcg ccaggatgat gacgattccc ggtaccacca tctgcaagtc
    21361 ctggttccag ccgaggatga tgatgccgtt gtcgatcacc ttgagcacca gcacgccgag
    21421 cagcgtgccg aacagcgagc cacggccgcc gagcaggctg gtgccgccaa ggatgacggc
    21481 ggcgatcacc gtcagctcga agccgcgccc agcggtcggc tggccggcat tcatcaatga
    21541 cgacaggatc atgccggcga cgccgacggc gaggccggtg acgacgaaag caagcagctt
    21601 cagccgttcc gaacgcaggc cggacaggcg caccgccctt tcattggcgc cgaccgcgta
    21661 gagatagcga ccgagcgtgg tgaagcgcag cgtcacatag gcgacgacga agatcagcgc
    21721 ggcgatgatg acgggtacgg gtaccgcgcc gacatagccg gcgccgagat cggtgaagct
    21781 tgccagttgg ttgtggttct gcacggcttc gcgcgtgaac agatagacgc cgccctgcag
    21841 catcatcatg gtgccgatgg tggcgatcag cgagttcacc ctgagcttgg tgacgacaag
    21901 cccgttgaac agaccgactc ccccggcgaa ggcaagcgct gccagcatgc cgagcgcgag
    21961 gctgtgcgtg gaattgagca ccgccatgcc gatgcagccg acaaaggcga gcgatgcgcc
    22021 gaccgacagg tcgacttccg ccgtgatgat gagcatggtc atgcccgagg cgacgatcag
    22081 caccagcgcg cattggcgca ggatggcgat gatgttggct tccgaataga actgcggcgc
    22141 cgcgatcgag atggcgatgc aaagcaccgc caggatggcg cccagcacca actg
//
