LOCUS       NZ_AP024109            20633 bp    DNA     circular CON 10-NOV-2023
DEFINITION  Mesorhizobium sp. J8 chromosome, complete genome.
ACCESSION   NZ_AP024109
VERSION     NZ_AP024109.1
KEYWORDS    RefSeq.
SOURCE      Mesorhizobium sp. J8
  ORGANISM  Mesorhizobium sp. J8
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1
  AUTHORS   Kusaba,I., Nakao,T., Maita,H., Sato,S., Chijiiwa,R., Yamada,E.,
            Arima,S., Kojoma,M., Ishimaru,K., Akashi,R. and Suzuki,A.
  TITLE     Mesorhizobium sp. J8 can establish symbiosis with Glycyrrhiza
            uralensis, increasing glycyrrhizin production
  JOURNAL   Plant Biotechnol. 38, 57-66 (2021)
  REMARK    DOI:10.5511/plantbiotechnology.20.1124a
REFERENCE   2  (bases 1 to 6690860)
  AUTHORS   Maita,H. and Sato,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2020) Contact:Shusei Sato Tohoku University,
            Department of Environmental Life Sciences, Graduate School of Life
            Sciences; 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/10/2023 07:29:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,439
            CDSs (total)                      :: 6,376
            Genes (coding)                    :: 6,302
            CDSs (with protein)               :: 6,302
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 48 of 74
            Pseudo Genes (incomplete)         :: 41 of 74
            Pseudo Genes (internal stop)      :: 12 of 74
            Pseudo Genes (multiple problems)  :: 21 of 74
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:17:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1699497
            Orig. end                         :: 1720130
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024109.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20633
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hserlactone"
                     /region_number="7"
                     /rules="Autoind_synth"
                     /tool="antismash"
     cand_cluster    1..20633
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="Autoind_synth"
                     /kind="single"
                     /product="hserlactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20633
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:10633](+)"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10633
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
     gene            complement(471..1625)
                     /locus_tag="MJ8_RS07690"
                     /old_locus_tag="MJ8_15210"
     CDS             complement(471..1625)
                     /EC_number="2.3.1.47"
                     /GO_function="GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (30-362). E-value:
                     3.6e-44. Score: 143.3. Matches aSDomain:
                     nrpspksdomains_MJ8_RS07690_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1109: 8-amino-7-oxononanoate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333932.1"
                     /locus_tag="MJ8_RS07690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15210"
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="WP_201413822.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4e-42, bitscore:
                     143.2, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGPPLARYEATLQGLARKDRLRRLAPRSGLDFSSNDYLGLASCG
                     RLGNAVAAAIARGTPVGATGSRLLRGNAPEHEELEADAAAFFGAERALFFGGGYIANFA
                     LLTALPQKGDLLVLDELAHASMHEGARAGRAEFVPVVHNDADALEDAIRRWRASGGMGR
                     IWIAVESLYSMDGDRAPLESLIALADRNEAFIVVDEAHATGIWGPDGRGLAAAFKGRDN
                     IVALHTCGKALGASGALVTGPRTLCDYLVNRSRPFIYATAPSPLMAVAAREALAVLVDE
                     PMRRVDLHERVAFAGRQFAERCGVRASGTQIQPVVIGDVRRTMAVAAELQAHGFDIRGI
                     RPPTVPEGTSRLRISLTLNVDEAATSAMMDALVDALAETRRMAT"
     aSDomain        complement(540..1535)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MJ8_RS07690_Aminotran_1_2.1"
                     /evalue="3.60E-44"
                     /label="MJ8_RS07690_Aminotran_1_2.1"
                     /locus_tag="MJ8_RS07690"
                     /protein_end="362"
                     /protein_start="30"
                     /score="143.3"
                     /tool="antismash"
                     /translation="LDFSSNDYLGLASCGRLGNAVAAAIARGTPVGATGSRLLRGNAPE
                     HEELEADAAAFFGAERALFFGGGYIANFALLTALPQKGDLLVLDELAHASMHEGARAGR
                     AEFVPVVHNDADALEDAIRRWRASGGMGRIWIAVESLYSMDGDRAPLESLIALADRNEA
                     FIVVDEAHATGIWGPDGRGLAAAFKGRDNIVALHTCGKALGASGALVTGPRTLCDYLVN
                     RSRPFIYATAPSPLMAVAAREALAVLVDEPMRRVDLHERVAFAGRQFAERCGVRASGTQ
                     IQPVVIGDVRRTMAVAAELQAHGFDIRGIRPPTVPEGTSRLRISLTLNVDE"
     gene            complement(1622..2563)
                     /gene="bioB"
                     /locus_tag="MJ8_RS07695"
                     /old_locus_tag="MJ8_15220"
     CDS             complement(1622..2563)
                     /EC_number="2.8.1.6"
                     /GO_function="GO:0004076 - biotin synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="bioB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF06968"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339721.1"
                     /locus_tag="MJ8_RS07695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15220"
                     /product="biotin synthase BioB"
                     /protein_id="WP_225248178.1"
                     /sec_met_domain="PF04055 (E-value: 2.4e-15, bitscore: 55.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="PF06968 (E-value: 1.1e-18, bitscore: 65.7,
                     seeds: 824, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWTLEKARALHDAPFNNLLFLAHTVHRQNFDPNKVQLSRLLSIKT
                     GGCPEDCGYCSQSAHHETGLKASKLMEVNRVIAEAIKARDAGATRYCMGAAWRNPKPRD
                     MDAVVAMVEGVKALGMETCMTLGMLDLDQAARLKQAGLDYYNHNIDTSERYYSEIVSTR
                     SFADRLETLENVRQSGIKVCCGGIVGMGEEKVDRIDMLVTLANLPEHPESVPINMLIPV
                     AGTPLAEATPIEPIEFVRVIALARIMMPKSRVRLSAGRTAMTDEMQALCFFAGANSIFV
                     GDTLLTAENPGEDKDTLLFRRLGIEPMELEAQ"
     gene            complement(2713..3564)
                     /gene="nadC"
                     /locus_tag="MJ8_RS07700"
                     /old_locus_tag="MJ8_15230"
     CDS             complement(2713..3564)
                     /EC_number="2.4.2.19"
                     /GO_function="GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /GO_process="GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nadC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581880.1"
                     /locus_tag="MJ8_RS07700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15230"
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="WP_201413824.1"
                     /transl_table=11
                     /translation="MNLSPLPAIMLEPLVRSALLEDLGRAGDVTTEATVAKYHHATTVL
                     AARQPGVVAGLDLVSLAFRLIDQNIKMDVNRADGSKVAEGEIIASVSGPTRAILTAERT
                     ALNFLCHLSGIATATASIVAAICEHRAKVTCTRKTTPGLRTVEKYAVRAGGGANHRFGL
                     DDAVLIKDNHIAVAGGIRSAIKRARESVGHLVKIEVEVDTLTQLEEALSLAPDAVLLDN
                     MSLDKLRQAVIIVAGRAITEASGRITATTAPAIAATGIDLMSVGWLTHSAPILDIGLDY
                     YT"
     gene            complement(3564..5105)
                     /locus_tag="MJ8_RS07705"
                     /old_locus_tag="MJ8_15240"
     CDS             complement(3564..5105)
                     /EC_number="1.4.3.16"
                     /GO_function="GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206217.1"
                     /locus_tag="MJ8_RS07705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15240"
                     /product="L-aspartate oxidase"
                     /protein_id="WP_201413825.1"
                     /sec_met_domain="DAO (E-value: 7.4e-08, bitscore: 30.9,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSANIRSFSGRPIIIGGGIAGLMTALQLAPEPVLLLSRTLLGADT
                     SSTSAQGGLAASFGQDDDPQLHLADTLAAGDGLCDTDMASRILQAAPGAIEGLARFGVP
                     FDRTPKGPLCLGLEAAHSRRRIVHAHGDGSGREIMRALIGAVRSTPSIVVVEGVEARRL
                     AVKENAIRGVWASCALGPVFFGSRRVVLATGGIGGLFLDTTNPSGSCGQGLALAARAGA
                     VLADLEFIQFHPTALECRRRPMALVSEAVRGEGAVLVDETGRRFLDGVPGAELAPRDVV
                     ARAVWSHLAGGHQVFLDARKRPGPEFQRRFPTIASACRKFGINPARALIPIRPAQHYHM
                     GGVAVDGSGRTSVKGLWACGEVASTGLHGANRLASNSLTEAVVCARWVAESVAGTPARR
                     TKPMFAGAYPSPDPIRVRPSLSRALGIVRNGEALKAAAQELLPLAECEAAASDPAAVGL
                     MIAISALRRRESRGAHYREDFPHHAAVARRSEITLEAALAAARELAHSPTLESTT"
     gene            complement(5102..6076)
                     /gene="nadA"
                     /locus_tag="MJ8_RS07710"
                     /old_locus_tag="MJ8_15250"
     CDS             complement(5102..6076)
                     /EC_number="2.5.1.72"
                     /GO_function="GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nadA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863266.1"
                     /locus_tag="MJ8_RS07710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15250"
                     /product="quinolinate synthase NadA"
                     /protein_id="WP_201413826.1"
                     /transl_table=11
                     /translation="MSGALPSAASLYRRVWRVIPPGEWAAFSDDIEAILALKRERNAVV
                     LAHNYQTPEIFHCVADIVGDSLALARKAMTVEAQVIVLAGVHFMAETAKLLNPDKTVLI
                     PDLWAGCSLADSITAQDVRLMRQRYPGVPIVTYVNTSAEVKAESDICCTSGNARKVVES
                     LGTPRVIMLPDEYLAHNIAAQTDVEIIAWKGHCEVHERFTPADIRELRESHPGVSVLAH
                     PECPPEVVAEADFSGSTAAMCDYVEKQKPPRVVLMTECSMSDNVALAHPDVEFIRPCNL
                     CPHMKRITLANIRAALEQNRHVVTIEPGIAGRARLAVERMLAI"
     misc_feature    6091..6111
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 25.36"
                     /tool="antismash"
     gene            complement(6143..7111)
                     /locus_tag="MJ8_RS07715"
                     /old_locus_tag="MJ8_15260"
     CDS             complement(6143..7111)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874735.1"
                     /locus_tag="MJ8_RS07715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15260"
                     /product="hypothetical protein"
                     /protein_id="WP_201415349.1"
                     /transl_table=11
                     /translation="MDNQAKGTRKHRTAVTADEAKVDLIAFVVAVSGNEPCVLTIGQAD
                     TLPSGPFALEHRSLQSGLRGWVEQQTGHPLGYIEQLYTFADRDRIGGERRQRAISISYL
                     ALTRKEQGADSSKCGWRSWYEYFPWEDHRSGAPPVVLDVLRPRLIEWADSAGDAAMRRE
                     RRQRAAKAFGFDDRHWNEELTLQRYELVYEAGLVQEAASGRDATRPSLVPSKSMVADHR
                     RIVATGIARLRSKIKYRPVVFELMPQSFTLLQLQRTVEALAGRLINKPNFRRLIEQQEL
                     VEETGETSVDTGGRPARLYRFRHAVLDETAMVGTKLPVARG"
     misc_feature    7259..7272
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.29"
                     /tool="antismash"
     gene            7284..8144
                     /gene="panB"
                     /locus_tag="MJ8_RS07720"
                     /old_locus_tag="MJ8_15270"
     CDS             7284..8144
                     /EC_number="2.1.2.11"
                     /GO_function="GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="panB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205540.1"
                     /locus_tag="MJ8_RS07720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15270"
                     /product="3-methyl-2-oxobutanoate hydroxymethyltransferase"
                     /protein_id="WP_201413827.1"
                     /transl_table=11
                     /translation="MSITGDSKAITPTDIRARKGGVPLVCLTAYTTPIAKLVDRHCDIV
                     LVGDSVGMVLHGLPSTLGVTLDMMILHGKAVRRGLEQALFVIDLPFGSYEASPEQAFRN
                     AARLMAETGCAAVKLEGGEAMAATIQFLAARGIPVMAHVGLTPQAVNALGGYRVQGRGE
                     DAARIRRDALSVAQAGAFSVVLEKMPEHLARRITGEIEIPTIGIGASPACDGQILVSDD
                     MLGLFSAFRPKFVKRYAELGDQASAAIAAYADEVRSRRFPAAEHVFADAAKGADVGPAG
                     IGGAA"
     gene            8141..9004
                     /gene="panC"
                     /locus_tag="MJ8_RS07725"
                     /old_locus_tag="MJ8_15280"
     CDS             8141..9004
                     /GO_function="GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="panC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533318.1"
                     /locus_tag="MJ8_RS07725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15280"
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="WP_201413828.1"
                     /transl_table=11
                     /translation="MSVPIARTVAGLRQILAGWRRNGASVAVVPTMGALHEGHLSLVRA
                     ALQTADRVIVTLFVNPKQFNSAADLAAYPRTENEDAAKLVPLGAHLLYAPHGEEMYPAG
                     FATTVSVSGVSEGLCGAFRPGHFEGVATVVAKLFLQTGADFGFFGEKDFQQLQLVRRMV
                     RDLDIPITVVACPTVREADGLALSSRNVRLSPAERAVAPKLPSVLSETAELLARGSPVL
                     STLAAAREAILAAGYREVEYLELCDEAELRPTTLLERPARLLVAAWLGGTRLIDNVAVS
                     PVGSTD"
     gene            9180..9905
                     /locus_tag="MJ8_RS07730"
                     /old_locus_tag="MJ8_15290"
     CDS             9180..9905
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1197:
                     autoinducer-binding transcriptional regulator,"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533317.1"
                     /locus_tag="MJ8_RS07730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15290"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_201413829.1"
                     /transl_table=11
                     /translation="MHRVFENFVEQLSASVDTVDLHEAMACVAAGFDFPLFAYFSYPSV
                     SGDAPRLISNYPSSWTSLYLQQRYHDLDPVILRGLQGWDTFDWGVELGRQYLPTSQQQI
                     LESAAELGIRGGLTISMHDHRGRFAALTFASDESRPALLQSLARYEKALQLMAINFHIH
                     VRHRLADDRVVDGVTLTPREFECLKWAARGKSAWDISQLLGLSKRTVTFHQENAKAKLG
                     VRTTNQAVARLAASGRARS"
     misc_feature    complement(9215..9230)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 21.73"
                     /tool="antismash"
     gene            10001..10633
                     /locus_tag="MJ8_RS07735"
                     /old_locus_tag="MJ8_15300"
     CDS             10001..10633
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hserlactone: Autoind_synth"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838483.1"
                     /locus_tag="MJ8_RS07735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15300"
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="WP_201413830.1"
                     /sec_met_domain="Autoind_synth (E-value: 5.2e-42, bitscore:
                     141.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMQLITPAFYGEFASELKEMHGLRYRVFKERLDWEVDSKDQMEAD
                     AFDGYMPVYLLLKGSDWRTCGCVRLLPSTGPTMLRDTFPVLLGADIVPSTPDIWESSRF
                     ALDLPSSAPKAAGGLAQATYELFAGMIEFGLANDLTRIVTVTDTRMERILRLATWPLSR
                     IGQPRPVGNTQAVAGFLEISQSSLLRIRDRGRLSGPVLWKPVLGVSI"
     gene            10930..11496
                     /locus_tag="MJ8_RS07740"
     CDS             10930..11496
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838477.1"
                     /locus_tag="MJ8_RS07740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2285 domain-containing protein"
                     /protein_id="WP_225248180.1"
                     /transl_table=11
                     /translation="MDATSARVFWDPRYCARVLRAAAEPADLDHDFEPLDLASLACGKD
                     ILLTPADVQQVLLRQDCRALQIAVSGASVLRPLRLYVDAIVPPRHLKFHVAALQFLNDI
                     KCSGRPAAACFPPEPRGGRLRIVLQALDGSLAAASHQEIAIALFGRGRVEEDWAHPGGH
                     LRDRVRRAIQRGRYLMGGGYRHFLT"
     gene            11694..12665
                     /locus_tag="MJ8_RS07745"
                     /old_locus_tag="MJ8_15310"
     CDS             11694..12665
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330287.1"
                     /locus_tag="MJ8_RS07745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15310"
                     /product="hypothetical protein"
                     /protein_id="WP_201413831.1"
                     /transl_table=11
                     /translation="MTVTKPDKADAASLEAIARNGSILRRIAVRIPTYLSDIRENPAWL
                     PMFVLARTMPARRLHWRGAKPVRAKKVQDTMFAGVNRQDVVDALQSDGLFSGLVLPKFI
                     HEEIAAFVRCTPCFGNFDRRLEFMPTEHEQAEARFGVPLLSGHYFERILACKAALAIQN
                     DPLLTDIAAHYLGGQAKLITTRVWWSFPTGQASDADKNLASLGKYHFDLDDWRMLKFFF
                     YLLPVDEGTGPHVYVRGSHKRRALKHQLTLLVGHPAEEVLDVYGEQSPVTLTGEAGLGF
                     VEDPFGFHMGTVPAHTPRLMMEVGFGVSPPSRRRFHGEPVIR"
     gene            complement(13713..14207)
                     /locus_tag="MJ8_RS07750"
                     /old_locus_tag="MJ8_15320"
     CDS             complement(13713..14207)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199588.1"
                     /locus_tag="MJ8_RS07750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15320"
                     /product="hypothetical protein"
                     /protein_id="WP_225248181.1"
                     /transl_table=11
                     /translation="MADRQPNQMIGRGPTSVNKPFGSVAITPVLRAITPMAAHELDSLV
                     FQMAVESARSLSLSFTEKAAAIAARSRGILLFDVRVDGDAAVQRIAAIRYPSDRTGVLA
                     LDRQGSVIRHCIVGGIFSGFIAPLENWTSMPLSMQAKINVDGHATLFLDALRKAGHMLV
                     S"
     gene            14586..15284
                     /gene="queC"
                     /locus_tag="MJ8_RS07755"
                     /old_locus_tag="MJ8_15330"
     CDS             14586..15284
                     /EC_number="6.3.4.20"
                     /GO_process="GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="queC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueC"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205545.1"
                     /locus_tag="MJ8_RS07755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15330"
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="WP_201413832.1"
                     /sec_met_domain="QueC (E-value: 5.3e-56, bitscore: 187.9,
                     seeds: 27, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTLVVCSGGLDSVSLAHKVAAEQELAGLLSFDYGQRHRKELGFA
                     ALCAERLRVPHRIIDVREIGSGLSGSALTDNIDVPEGHYAEDTMKVTVVPNRNAIMLAI
                     AFGFAAARSADAVAMAVHGGDRFIYPDCRPAFIDAFQVMQDRALDGYGNVRLYAPYVNL
                     TKADIVEDGARHGTPFAQTWSCYKGGERHCGRCGTCVERREAFDLSGQADPTDYDDADF
                     WLGALRRRGV"
     gene            15284..15640
                     /gene="queD"
                     /locus_tag="MJ8_RS07760"
                     /old_locus_tag="MJ8_15340"
     CDS             15284..15640
                     /EC_number="4.1.2.50"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="queD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTPS"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528526.1"
                     /locus_tag="MJ8_RS07760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15340"
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="WP_201413833.1"
                     /sec_met_domain="PTPS (E-value: 1.1e-34, bitscore: 117.5,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFRITKEFHFSASHQLTSLPPDHQCARLHGHNYIVEVELSAKELN
                     EHGFVRDYHDLAPLKRYIDDNFDHRHLNDVLGHEQVTAECLARHFYEWCKARLPETSAV
                     RVSETPKTWAECRP"
     gene            15637..16371
                     /gene="queE"
                     /locus_tag="MJ8_RS07765"
                     /old_locus_tag="MJ8_15350"
     CDS             15637..16371
                     /EC_number="4.3.99.3"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="queE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533305.1"
                     /locus_tag="MJ8_RS07765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15350"
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="WP_201413834.1"
                     /sec_met_domain="QueE (E-value: 3.2e-45, bitscore: 153.0,
                     seeds: 32, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDTHPAIRVSEIFGPTIQGEGVLIGLPTVFVRTGGCDYRCSWCD
                     SLHAVDNQFRHEWEMMSSDAVWQNVVALSGGRPLMVSLSGGNPAIQPFGALIKRGHNEG
                     YRFALETQGSVAREWFADLDVLVLSPKPPSSEMTTDWEAVEACLQAARDKPEIVLKLVV
                     FNESDYAYAKEAAARFPRLPIYLQPGNHTPPRPGNEDAPVDLDGITKRMEWLVDSVTRD
                     RWFEARVLPQLHVMLWGNKRGV"
     gene            complement(16595..17944)
                     /gene="hemN"
                     /locus_tag="MJ8_RS07770"
                     /old_locus_tag="MJ8_15360"
     CDS             complement(16595..17944)
                     /EC_number="1.3.98.3"
                     /GO_function="GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemN"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329232.1"
                     /locus_tag="MJ8_RS07770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15360"
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="WP_201413835.1"
                     /sec_met_domain="PF04055 (E-value: 3.9e-18, bitscore: 64.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRPELAARLGENVPRYTSYPSAPHFHAGIDAVVYRGWLEALEGGG
                     EISLYLHIPYCDRLCWFCACHTKHTRHYEPVAAYLRSLHAEFATVGRLVSGRGRVRAVH
                     LGGGSPTMLRPQHMVILGKALRDNFDFVHDAKISVEIDPNDMDGARLDALAQIGMTRAS
                     LGVQDFDPKVQKAINREQSFLQTKAVVDGLRSRGVDSVNLDLLYGLPHQSSESVRSTVE
                     QALTLEPDRMALFGYAHVPWFKKHQTMIDEAWLPGPSERFAQSRLAARTIEDKGYESIG
                     LDHFAKPGDALAIAARAGALHRNFQGYTEDRCETIIGLGSSSIGRFRQGYVQNMTSTGD
                     YRRRVEDGELAAVRGIAFSEDDRVRGWIIERLMCDFAFSAIDLVESFGKAGQRLLRQAS
                     SIALEDPARLLELQGDSFVVPAENRPFVRSIAVKFDKYFESGTARHSAAV"
     gene            18112..18876
                     /locus_tag="MJ8_RS07775"
                     /old_locus_tag="MJ8_15370"
     CDS             18112..18876
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914028.1"
                     /locus_tag="MJ8_RS07775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15370"
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_201413836.1"
                     /transl_table=11
                     /translation="MTVRHDIHHSGIPVYCLSCQARQRGLCSALDADRLAKLAKTSSRH
                     RVEAGVQLIGDTEAVNSYSNVLSGVVKLTKSLSDGRQQIVGLQFAPDFLGQPFKVKSAT
                     HAEAATTVSLCSFPKAAIEQMIKQSPELEHRLLKQTLYELDEAREWMVTLGCKTAPEKV
                     ASFLLMIARNIGSSVGPTATSTCFDLPLTRADISDFLGITNETVSRQLTRLRMDGVIRI
                     ENNRHVTVDNMSRLEQRCGGRGPRRNRHVNKI"
     gene            complement(19213..20631)
                     /locus_tag="MJ8_RS07780"
                     /old_locus_tag="MJ8_15380"
     CDS             complement(19213..20631)
                     /EC_number="1.2.1.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014989302.1"
                     /locus_tag="MJ8_RS07780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_15380"
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="WP_225248297.1"
                     /sec_met_domain="Aldedh (E-value: 8.7e-168, bitscore:
                     557.1, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASVPHLSATSGAGRFAVHNPSTGELLAELPDMETADVAVAIDRA
                     DAARACWAGLTARERSDALWQWHQLILEHSDDLAAILTAEMGKPLAEAKSEIAHAAAYL
                     QWYAEEANRIYGETISAPSKDRRMLVIKQPIGVVGAITPWNFPASMVARKISPALAAGC
                     TVVLKPAEQTPLVAGAMFALARLAGFPDGVLNLVYASEGDGVGRELCANPKVRKISFTG
                     STEVGRLLMRQCSGQIKKVSFELGGNAPFIVFDDADVDAAVEGAIQAKFRNAGQTCVSA
                     NRLYVHSSVHDVFAEKFAELVQALRVGDGFEADVAIGPLIDIHALRKIEAHVSDAVLKG
                     ATIRCGGNRIGQSGTFFEPTVITGVISTMQIAQEETFGPLAPIIRFDDPDEVVRAANDT
                     IYGLAAYFYASNLKRVWRVAEALEYGMVGINTGRMSSEAAPFGGLKQSGIGREGSRHGL
                     EDYLEMKYLCMGGL"
ORIGIN
        1 gcgggatgga gcgcgctcgc agcgcctcga tcgacaacag tgtgtgattg atggtgccga
       61 gcgcggtgcg cgcgcacaag atgacgggca gttgccattc ccggaaaacg tcgatgaatt
      121 ttgtctgccg attgagcggc accatcagcc ccccagcacc ttcgatgatg agcggaccag
      181 gcaagtgtgg aagcgagagc tttgccgcct cgatcgcgac gtcgtcgatt tcggcggaac
      241 ggtgcggcga aagcggcgcc ctcaagcgat aaacttccgg cagcacgcgc ccggccggta
      301 gcccggaaag gcgggcaacg acctcgctgt ccgtctcctc gtcgaggccc gattgtaccg
      361 gcttccagta gaagccgtcg agcacgccgg cgaggcccgc tgaaaacacc gtcttgccga
      421 tgccggtgtc cgtcccggtg atgacaatgc ctgcggtcat ggtgctctgg tcatgtcgcc
      481 attcttcggg tttcggccaa agcatcgaca agcgcatcca tcattgccga ggtggcggct
      541 tcatcgacat tgagcgtcag cgatatgcgc aggcgcgatg ttccctccgg caccgtcggc
      601 ggccgaatgc cgcgaatgtc gaagccgtgt gcttgcagtt ccgccgccac cgccatggtc
      661 cgcctgacgt cgccgatgac aactggctgg atctgcgtcc cgctggccct taccccgcag
      721 cgctccgcaa attgacggcc agcgaaagct acgcgctcat gcagatcaac gcggcgcatc
      781 ggctcgtcga cgagcactgc gagcgcttca cgcgcggcca cggccatcag cggcgatggc
      841 gcggtggcgt agatgaatgg ccgggagcga ttgacgagat agtcgcacag cgtcctcggt
      901 ccggtgacga gcgcgccaga cgcgccgagc gctttgccgc atgtgtggag cgcaacaata
      961 ttgtcgcggc ctttgaaagc ggcagccaac ccccggccat ccggccccca gatgccggta
     1021 gcgtgagcct cgtcgacgac gatgaacgcc tcgttccgat cggcgagcgc gatcaggctc
     1081 tccaacggcg cgcggtcgcc atccatgctg tagaggcttt cgacagcgat ccagatccgc
     1141 cccatgccgc cactggcgcg ccagcgcctg attgcatcct cgagggcgtc ggcgtcgttg
     1201 tgcacgaccg gcacgaactc cgcgcgcccg gcgcgcgcac cttcatgcat gctggcatgg
     1261 gcaagctcgt cgagaaccag caggtcgccc ttctgaggca gggcggtcag cagtgcgaaa
     1321 ttggcgatgt agccgccgcc gaagaagagc gcgcgctcgg cgccgaagaa cgcagccgcg
     1381 tcggcctcca gttcctcatg ctccggtgcg ttgccgcgca gcagccgcga ccctgtggcc
     1441 cccaccgggg ttccgcgggc aatggccgcc gcaaccgcgt tgccaagcct cccgcagctg
     1501 gcaagcccga gatagtcgtt cgacgagaag tcgagaccgg agcgcggagc aagccgccgt
     1561 agccggtcct tgcgcgccag cccctgcaag gtcgcctcat accgggcgag gggaggcccg
     1621 ttcattgcgc ctcgagttcc atcggctcga tgccaagacg ccggaacaga agcgtgtcct
     1681 tgtcctcgcc gggattttcc gccgtcagca gcgtgtcgcc gacgaagatc gaattggcgc
     1741 cggcaaaaaa gcacaacgcc tgcatctcgt cggtcatggc ggtgcggccg gccgaaagcc
     1801 ggacacgcga tttcggcatc atgatgcgcg ccagtgcgat cacgcgcacg aactctatcg
     1861 gctcgatcgg cgtggcttcg gcaagcggcg tgccggcgac cgggatgagc atgttgatcg
     1921 gaacgctttc tggatgctca ggcagattgg ccagcgtcac cagcatgtcg atccggtcca
     1981 ccttctcctc gcccatgccg acaatgccgc cgcagcagac cttgatgccc gattggcgaa
     2041 cgttctccag cgtctcgagc cgatcggcaa agctgcgcgt gctgacgatc tcgctgtagt
     2101 agcgttccga cgtgtcgatg ttgtggttgt agtagtcgag ccccgcctgc ttcagccgcg
     2161 ctgcctggtc gaggtcgagc atgccgagcg tcatgcaggt ctccatgccg agcgccttga
     2221 cgccttcgac catggcgacc accgcatcca tgtcgcgcgg cttgggattg cgccaggccg
     2281 cacccatgca atagcgggtg gcgccggcgt cccgcgcctt gatcgcctcg gcgatgacgc
     2341 ggttgacttc catcagcttc gaggccttca gccccgtttc gtgatgcgcc gactggctac
     2401 aatagccgca atcctccggg cagccgccgg tcttgatgct gagaagccgg ctgagctgga
     2461 ccttgttggg atcgaaattc tgacggtgaa cggtatgtgc caggaagagc agattgttga
     2521 aaggtgcgtc gtgaagggcc cgagccttct ccagggtcca catcccgcca tcagcgcatt
     2581 gcgtccgtgg catcgtctca gtcggatcgg tgtcgtgcat atccgtcctg cttctcagct
     2641 tctttgcttg ccgtgactct tcgtctgatt catgccggtt tgattgtcga tccccgaccg
     2701 tttcgctgcg gatcatgtgt aatagtccag tccgatatcg agaattggcg cactatgggt
     2761 cagccagccg acggacataa ggtcgatgcc ggtggccgcg atggctggcg ccgtcgtggc
     2821 tgtgatcctg cccgacgcct cagtgatcgc ccggccggca acaatgatca ccgcctgccg
     2881 aagcttatcg agcgacatgt tgtcgagcag gacggcatct ggggcgagcg agagggcttc
     2941 ttccaactgg gtcagcgtgt cgacctcgac ttcgatcttg acgagatggc cgacgctctc
     3001 tcgcgcgcgc ttgatggcgg agcggatgcc gccggcaacc gcaatgtggt tgtccttgat
     3061 cagcacggcg tcatcgaggc cgaagcggtg attggcgccc ccgccggcgc gtacggcgta
     3121 tttttcgaca gtccgcaacc cgggggtcgt cttacgggtg caggtgacct tggcgcggtg
     3181 ctcgcaaatc gctgcgacaa tcgaagcggt tgccgtggca atgccactga gatgacataa
     3241 gaaattcagc gccgtgcgtt cagcggtgag aatggcccgg gtcggaccac ttaccgatgc
     3301 aattatctcc ccttccgcga ctttgctgcc gtccgcccgg tttacgtcca tcttgatatt
     3361 ttggtcgatc aacctgaatg cgaggctcac cagatcgagc ccggcgacaa ccccgggctg
     3421 gcgtgcggca agcactgtcg tggcgtggtg atacttcgct acggtcgcct cggtggtgac
     3481 gtcaccggct ctgcccaggt cttcgagaag ggctgaccgt accagcggct cgagcataat
     3541 tgcgggaagc ggtgacaggt tcatcaggtg gtgctttcca gcgttggcga gtgtgccagt
     3601 tcccgcgctg cggccaaggc ggcttcgaga gtgatttcgg accgtcgcgc aacagccgca
     3661 tgatggggaa agtcctcgcg gtaatgagcg ccgcggctct ctcggcgccg caaggcggaa
     3721 atagcaatca tcagaccgac agccgccgga tcggatgccg cggcctcgca ttcggcgagc
     3781 ggcaacaact cctgagcggc tgcttttaaa gcttcgccat tgcgcacgat gccgagcgcg
     3841 cgcgacaggg aaggacgcac acgtatcgga tctggcgatg gataagcgcc cgcaaacatt
     3901 ggctttgttc tgcgagcagg cgttccggcc acgctttcag cgacccagcg cgcgcacacg
     3961 acggcctcgg tcaaggaatt gctggcgagc ctgttggcgc catgcagtcc agtcgaggca
     4021 acttcaccac aggcccacaa ccccttgacc gacgtcctac cggagccgtc cacagcaaca
     4081 ccacccatgt gatagtgctg ggcaggacga atgggaatga gagcgcgcgc gggattgatg
     4141 ccgaatttac gacaggcaga cgcgattgtg gggaagcgtc gttgaaattc cgggccaggg
     4201 cgtttgcgcg cgtcgaggaa aacctgatgg ccgccagcca gatggctcca gactgcgcgg
     4261 gcaacgacgt cgcgtggcgc aagttcggcg cccggcacgc catcaaggaa gcgccgaccc
     4321 gtctcgtcga ctaggacagc accttcgcct cgaacagcct cgctgaccag agccatgggg
     4381 cgtcgccggc actcaagcgc ggtcgggtgg aactggatga actcgaggtc ggcaaggaca
     4441 gctcccgcgc gggccgcaag tgccaggccc tgtccgcaac tgcccgaagg attggtcgta
     4501 tcgagaaaga gcccgccgat cccaccggtg gcaaggacga cccgccgtga gccgaagaag
     4561 acaggtccca gcgcgcagct cgcccagacg cctcggatgg cattctcctt gaccgccagt
     4621 cggcgcgctt ccacgccctc tacgacaacg atcgagggtg tggagcgcac ggccccaatc
     4681 agtgcacgca tgatctcacg cccactgccg tcgccatgcg catgaacgat gcgacgccgg
     4741 ctgtgcgcgg cctccaatcc gaggcacagt ggtcctttcg gcgtacggtc gaagggaacc
     4801 ccaaagcgcg caagcccttc gatcgcccct ggcgcggctt ggaggatgcg gctcgccatg
     4861 tctgtgtcac acagaccgtc tcccgcggcg agcgtgtcgg ccaagtgcaa ttgcggatcg
     4921 tcgtcttgac cgaaactcgc agccagcccc ccctgtgccg aggtgctcga tgtgtctgct
     4981 cccaagagcg tcctggacaa aagaagcacc ggctccggcg ccaattgaag cgcggtcatc
     5041 agcccggcta ttccgccgcc gatgatgatg ggccgcccgg agaagctgcg gatattggcg
     5101 ctcaaattgc cagcatccgt tcgaccgcaa gccgcgcgcg tccggctatt cccggctcga
     5161 tggttacgac atggcggttt tgctcaagcg ccgcgcggat gttggcaagc gtgatccgct
     5221 tcatgtgcgg gcacaaattg cagggccgga tgaactcgac atctggatgc gctaacgcga
     5281 cgttgtcgct catcgagcat tccgtcatca gcaccacacg tggcggcttt tgcttttcga
     5341 catagtcgca catggctgcc gtcgaaccgg aaaaatcggc ctcggccacg acctcgggcg
     5401 ggcattcggg atgggccagc acgctcacgc cgggatggct ttcgcgcaac tcgcggatat
     5461 cggccggcgt gaagcgctca tgcacctcgc aatggccttt ccaggcgatg atctcgacgt
     5521 cggtctgggc ggcgatattg tgggccagat attcatccgg caacataatg accctggggg
     5581 taccaaggga ttcgacgacc ttcctggcgt tgcccgacgt acagcagata tccgactcgg
     5641 ctttcacttc ggccgacgtg ttgacatagg ttacgatcgg cacgccggga tatcgctggc
     5701 gcatcaagcg gacatcttgt gccgtgatcg agtcagctag cgagcaacca gcccagagat
     5761 cgggtatgag gaccgtcttg tccggattaa ggagcttggc cgtttcggcc atgaagtgca
     5821 cgccggccag cacgatgacc tgcgcctcga ccgtcatagc cttgcgggcg agcgccaagc
     5881 tgtcgccgac gatgtcggcc acacagtgga agatctcggg ggtttgatag ttgtgggcca
     5941 ggacgacggc attgcgctcg cgcttcagcg ccagaatggc ctcgatatca tcgctgaaag
     6001 ccgcccattc ccccggcggg atgacgcgcc agacgcgtcg atacagagag gcggccgaag
     6061 gtaacgcccc gctcatcggc gcctccccat ttataatcca tttgactata aggagtatat
     6121 atctaggttg actacaagcc tgtcaacccc gcgcgaccgg caattttgtg ccgaccatcg
     6181 ccgtttcgtc gaggaccgca tggcggaaac gatagagcct tgccggtcgc ccgcccgtgt
     6241 cgacggaagt ctcaccggtc tcctcgacca gctcctgctg ctctatgagg cgacgaaaat
     6301 tgggcttgtt gatcagcctg ccggcgagcg cctcgacggt tcgttgcagc tgtaacagcg
     6361 taaaagattg cggcatcagc tcaaagacga ccggccgata tttgatcttg gagcgcagtc
     6421 gcgcgatgcc cgtcgccacg atgcggcgat gatcggcaac catggacttg ctgggcacca
     6481 gcgatggccg cgtcgcatcg cgtccgcttg ccgcctcttg gaccagccct gcttcgtaga
     6541 ccaactcata gcgctgaagc gtaagttcct cgttccaatg gcggtcgtcg aagccgaagg
     6601 ccttcgccgc ccgctgccga cgttcgcgtc gcatggccgc gtcgccggca ctatctgccc
     6661 attcaatcaa gcggggccgc aatacgtcca gcaccaccgg cggtgcgccg gagcggtgat
     6721 cctcccatgg aaaatattcg taccagcttc gccagccgca cttggacgag tccgcaccct
     6781 gttccttgcg cgtcagtgcc agatagctga tggagatggc gcgctgccgg cgctcgccgc
     6841 cgatgcggtc tcgatctgcg aaggtgtaga gttgttcgat gtagcccagc ggatggccgg
     6901 tctgctgctc gacccaccct ctcaaaccgg attgcagcga tcggtgctca agcgcaaatg
     6961 gtccggaagg caatgtgtcc gcctgcccga ttgtgaggac acagggttca ttgccggata
     7021 cggcgacgac aaaggcgatc aggtcaactt tggcttcgtc cgccgtgacg gcggttctgt
     7081 gtttccttgt tccttttgcc tgattgtcca tgttcgcgaa gtggtttgca ggcgctcggc
     7141 gccttaatcg atttagttta agcacggctg tctgcacgtc aacagcgcgc agctgcacgg
     7201 ccaattttta gctcttcgca cttgcacaaa aacatgccaa acgcgacacc tgcggtgggt
     7261 aacgctgcgg aaggaagaat gccttgagca taactggcga ttcaaaggcg attacaccga
     7321 ccgatattcg ggcgcgtaag ggcggcgtcc cgctcgtctg cctcacggcc tatacgacgc
     7381 cgattgcaaa gctggtcgat cgacattgcg acatcgttct tgtcggcgac agcgtcggca
     7441 tggtgctgca cggcctgccg tcgacgctcg gcgtcacgct cgacatgatg attctgcacg
     7501 gcaaggcggt gcggcgtggc ctggagcagg cgctgttcgt cattgacttg ccgttcggtt
     7561 cctacgaggc aagtcccgag caggcgttcc gcaacgcggc tcgactgatg gcggagaccg
     7621 gttgcgcggc tgtcaagctg gagggcggcg aggcgatggc cgctactatc caattcctcg
     7681 ccgctcgtgg cattcctgtc atggcccatg tcggcttgac gccgcaggca gtcaacgcct
     7741 tgggtggtta ccgcgtgcag ggcaggggag aggacgccgc acggatcagg cgcgatgcgc
     7801 tgtcggtcgc gcaagccggc gccttttcgg tcgtgcttga aaagatgcca gagcacctcg
     7861 cgcggcggat cactggcgag atcgaaatcc ccactatcgg catcggcgcc tcaccggcct
     7921 gcgacggcca gatcctggtg agtgacgaca tgctcggcct gttctccgcg ttcaggccga
     7981 aattcgtcaa gcgctacgca gagctcggcg atcaggccag cgccgcgatc gcggcctatg
     8041 cggacgaagt gcgcagcagg cgcttccccg ccgccgagca cgtctttgcc gacgcggcca
     8101 agggggccga cgtaggtccg gcaggaatcg gaggagcggc gtgagcgtgc cgattgccag
     8161 gaccgttgcc ggacttcgcc aaatactcgc cgggtggcgc cgaaacggcg ccagcgttgc
     8221 cgtcgtgccg accatgggcg ccttgcatga agggcatttg agcctcgtac gtgccgcgct
     8281 ccagaccgct gatcgtgtga tcgtgacgct gttcgtcaat ccgaagcagt tcaacagtgc
     8341 cgccgacctt gccgcctatc ctcgcacgga aaacgaggat gccgcaaagc tcgtgccgct
     8401 gggagcgcat ctgctctatg cgccgcatgg cgaggagatg tatccggcag gctttgccac
     8461 gacggtttcg gtgtccggcg ttagcgaggg cctgtgcggt gcttttcggc cgggtcattt
     8521 cgagggcgtg gcgactgtgg tggccaaact cttcctgcag acaggcgccg acttcggctt
     8581 ctttggcgaa aaggactttc agcagcttca gctcgtcagg cgcatggtac gggatctgga
     8641 tattccaatt accgtcgttg cctgtccgac tgtccgcgaa gccgacggtc ttgcactgtc
     8701 atcgcgcaat gtccggcttt cgccagccga gcgcgctgtt gctcccaagc tgccatcggt
     8761 cttgagtgag acggccgagc tgcttgcccg cggctcaccc gtcctgtcaa cgcttgccgc
     8821 agcgcgcgaa gcaattctgg ctgcaggcta tcgcgaggtc gaatacctgg aactctgtga
     8881 cgaggcagag ctgcgaccca cgacactcct tgagcgaccg gcacgcttgc ttgtcgcggc
     8941 ctggcttggc ggcactaggc ttatcgacaa cgtcgcagta tcaccggttg gttcaacgga
     9001 ttaacgaggt tctccgcgtt gcatagcagt cttgaaagag gcttcttcaa ggtaaggctg
     9061 gacacaacgg cggtgatgac tgtgaaaacg tcgcgcgcat tgcatcggcg cggggtcgtt
     9121 tagtccaagg ctatgacttg gtatcgaagc gagattcgcg gaggtcacgc aaagcgcaaa
     9181 tgcatcgcgt atttgaaaac ttcgtcgagc aactgtctgc aagtgtagat actgtcgatc
     9241 tgcatgaggc catggcgtgc gtcgccgccg gcttcgactt cccgctcttt gcctatttca
     9301 gttatccgtc ggtatccggc gacgccccgc gattgatctc aaattacccc tcatcctgga
     9361 catcacttta cctgcaacag cgctaccacg atcttgatcc ggtcatcctg cgtgggctgc
     9421 agggctggga tacctttgat tggggcgtgg agctcggtcg acagtatctg ccgacttcgc
     9481 agcagcaaat tctggagagt gcggctgagc ttggcatccg cggcggattg acgatatcca
     9541 tgcacgatca tcgcggccgt tttgctgccc tgacctttgc ttccgacgag tcacgtccgg
     9601 ctcttttgca gtcattggca cgttacgaaa aagcactgca gttgatggct atcaactttc
     9661 acatccatgt caggcacagg ctcgccgacg atcgcgtggt ggacggtgta acgctcaccc
     9721 cgcgggagtt cgagtgtctg aaatgggcgg cgcgcggcaa gtcggcttgg gatatcagcc
     9781 agctactcgg gctctcgaag cgcaccgtga ccttccacca ggaaaacgcc aaagcaaagc
     9841 ttggcgttcg aacgaccaat caggccgttg cgcggctagc tgcctcaggg cgggcgagat
     9901 cctaattctt ctgccacgat agctgtaaac gtctacaggc tgcattgctg tcgcctttgc
     9961 cctccgatga cggcaacaca tcccgcacgg aggcgcgacc atgatgcagc tgataacacc
    10021 ggccttttac ggcgagttcg caagcgagct caaggagatg cacgggctca gatatcgagt
    10081 cttcaaggaa agactagatt gggaagttga ctccaaggac caaatggaag cggacgcgtt
    10141 tgatggctac atgcctgtct atctgcttct caagggatcc gattggcgaa cttgcggttg
    10201 tgtgcgtctt ttgcccagca ccggacccac aatgttgcgc gatacgttcc ctgtgctgct
    10261 gggtgctgac attgtgccct cgacgcctga catatgggag agcagtcgtt tcgcgctcga
    10321 tcttccttcc tccgcgccca aggcagccgg cgggttggcg caagcaacct acgagctctt
    10381 tgccggcatg atcgagttcg gcttggccaa tgatctcacc cggatcgtca cggtgaccga
    10441 tacgcgcatg gaacggatcc ttcgcctcgc aacgtggcca ttgtcacgca tcggacaacc
    10501 caggcccgtc ggcaacacgc aggcggtggc cggatttctc gagatttccc aatcgagcct
    10561 tttgcgcatc cgcgacaggg gacgtctgag cggaccggtc ctgtggaaac cggttctagg
    10621 cgtttcgata tagcgcgatt gcgggcgcct gagccgtgac gaatgcacgc ctgctccagg
    10681 acgccacggc cgctcgcgca ttctggccgg actggcgtcg tcacgaaact taccactaca
    10741 cgagacgatt gccgcgtacg ggctgggcct gggaatttct tcggcgcaat ccgcagttcc
    10801 agcgcgatca cgccaaaatg gcagcgcccg cctccactgt cggggccggc ggtacgaatg
    10861 taatcgagct gacgacggga tcacttttcc tcagccaatg gagcctcctt tttcgccgac
    10921 gagccaaaat tggacgccac gtcggcaagg gtcttctggg acccgcgcta ctgcgcccgc
    10981 gtcctgcgag cggccgccga gccggctgat ctcgatcacg attttgaacc gcttgatctt
    11041 gcctcgctcg catgcggcaa ggacatcctg ctgacgccgg ccgatgtcca gcaagttttg
    11101 ctgagacagg actgccgggc tctgcagatc gccgtttccg gcgcctccgt tctgcgcccg
    11161 ttgcggctat acgtagacgc aatcgtgccg ccgcggcact tgaagttcca tgtcgctgcc
    11221 cttcagttcc tcaacgacat caagtgtagc ggccgcccgg cagccgcttg ctttccgccg
    11281 gaaccccgcg gcggacgcct gcgtatcgtg ctccaggctt tggatggttc gcttgcggca
    11341 gcatcccatc aggagatcgc cattgcgctg ttcgggcgag gacgtgtcga agaagactgg
    11401 gctcatccgg gcggccatct tcgcgatcga gtgcggcgcg ccatccagcg tggccgatac
    11461 ctgatgggcg ggggttacag gcattttctg acgtagccac tgtgatcgcc tcgtaaggct
    11521 tgttcgacag gcgcgcagtg acgtgtgcat tgccggtgag gtaattgtgc gttgcacaaa
    11581 agttgacgcg cggctagcgt tctaccgctg cgggaatcgg gcttgggtaa tacgggacga
    11641 agcagtcagg accaatgctt cgtcgatagt gcattcatac gctgaggatc ctgatgacag
    11701 ttacgaaacc cgacaaagcc gatgctgcaa gcctcgaggc gatcgcgcga aatggcagta
    11761 ttctacgtcg catcgcggta cgtattccga cctatctgtc ggatattcgc gagaacccag
    11821 cgtggctgcc gatgttcgtg ctcgcccgga ccatgccggc acgcagactg cactggcgtg
    11881 gtgcaaagcc cgttcgagcg aagaaggtcc aggacacgat gtttgccggc gtcaatcgcc
    11941 aggacgttgt ggacgcgctg cagtcggatg gacttttttc cggtctcgtc ttgccgaaat
    12001 ttatccatga ggaaatcgca gctttcgtcc ggtgcacacc ttgctttggc aatttcgatc
    12061 gccgtctcga attcatgccg acggagcatg agcaggctga ggctcggttc ggcgttccgt
    12121 tgctcagcgg ccactatttc gagcgcattc tcgcctgcaa ggcggcactt gccatccaaa
    12181 acgacccgct gctcacggat atcgctgcac actatctcgg cggccaggcg aaactgatca
    12241 caacgcgtgt ttggtggagc ttccccacag ggcaggcttc cgacgccgac aagaacctgg
    12301 catcgctagg aaaataccac tttgacctcg acgactggcg aatgttgaag ttcttcttct
    12361 acctgctgcc ggtcgacgag ggcaccggtc cgcatgtcta cgtccgcggc agccacaagc
    12421 gccgcgcgct caagcaccag ctgacgcttc tcgtcggcca ccccgcggaa gaggttctcg
    12481 acgtctatgg cgagcaaagc cccgtgactc ttaccggaga ggcggggctt ggcttcgttg
    12541 aggatccttt cggctttcac atgggcaccg tcccggcgca cacgccgcgg ctgatgatgg
    12601 aagtgggctt cggcgtttcg ccgccctcgc gccgacgctt ccacggcgag cccgttattc
    12661 gctgatttcg aagaagtccc gagacagctt ttcccagacc gaagaccagt atctcgcggg
    12721 caaataagca acggccccat cccggaaaaa tcccgtttcc gccgcatggt gccgttgcaa
    12781 ctatttttca tgcctaggca gtccacctcg cgaagacccc cctaggacgc cggcgcaacg
    12841 aaacccaatc gactgataaa gctccggttt ctgcgctttg tgagctgaca aacttgtcca
    12901 gaaattggct cttgtgtacc cgggtttggc tgccgacacg gccttgcatt cgcgcaccgc
    12961 tattacggca atacgaaggg caccgggacg ctgttgggtt gactatgggt gtcggaaccg
    13021 cgaaaaggga cgcgctcttc gtgagtggcg tgcgccggcg gtctaaaccc cccaagcccc
    13081 ccgccagacc gccggcgcgc ttctaggaat gtcgaggaga ccgcgcccaa ggcaccttgt
    13141 ccgactatgc ttgatcggat tctcaatcgg tgtaaccgtc gcatgcggct ctactggcga
    13201 actaatcctt gcgcttcctc aagcgaggaa agccgatcac catgagtgcg tcagtttggt
    13261 gtttgtccac ctgccgtctg cgatctcgtg tccacgtcgc cgagttaaaa gtggaagctc
    13321 ctgttgatcg aaaaagggca ggccggcttg gtgccctttt gtcggctcct tttttgcttc
    13381 gttcgagttt tgccccgcgt cgggacgctc gacttccgac aagaggaggc gattgagctc
    13441 gatactcacg ccaaacaact cagcgcggta agctccatcc cggggagcta ggacgcagtc
    13501 agacttgcgc ttgcgaaccg cctgtagact tgctcgaagc cagctagaga cttgatctaa
    13561 acgatccagc aacgatattg gcgcgcattc gcctagttgc tcgccgccat cgctttggcg
    13621 cgatcaccgc agccgtttcg aacacaacag tcacgccgac aatgtcggca ccggatgcca
    13681 caaaaagtca caagatgcat ttcagcaaaa cgctagctga ccagcatatg tccggccttt
    13741 cgcaacgcgt ccaggaagag agtggcatgg ccgtcgacat taatcttggc ctgcatcgag
    13801 aggggcatgc tggtccaatt ctccaatggc gcaatgaaac ccgagaaaat tccaccgaca
    13861 atgcaatggc ggataacaga gccttgtctg tccaaagcca gcacgccggt ccggtcgctt
    13921 ggatatcgta tggccgcgat ccgctgcacc gccgcgtcac cgtcgacccg cacgtcgaaa
    13981 agcaggatac cgcggctcct ggcggctatc gccgcagctt tttcagtgaa gcttaaagag
    14041 agtgagcggg cgctttcgac cgccatctgg aaaaccagcg aatccagttc atgcgcagcc
    14101 atgggggtaa ttgcccgcaa cacaggagtg attgcaaccg acccaaacgg cttgttcaca
    14161 gatgtcggcc cacggccgat catttggttt ggttgacgat cggccatcga tcgacggcgt
    14221 gctccgattc gaaccgatcc tgagatggaa gacgcggggt gttagcgttg gcatcggctc
    14281 ggcggctacg aactccgttc gcttccatcg tccaagctag gcactacttg gcgcttgatc
    14341 gaagcgtcga gaatcttggt tccgaaggtc ctggcctgcc tcagagccta ttttggcacg
    14401 gcttcgaaat gcggtgaatt gggccggttc gcagcttagt gtttcttgca ggctggccgg
    14461 cctgagcaca gcggtgcgtc agcgcatcaa cttcgtcccg gttcacaaac gagactccgt
    14521 agggtagtgg gagcgcagag aaatctccgg tcgcagccta cccggtcaaa acaggaaacc
    14581 ccaccatgaa gactctcgtc gtctgctccg gcggattgga ctccgtttcc cttgctcaca
    14641 aggtggcagc ggagcaagaa ctcgccggcc tcctatcgtt cgattacggc caacggcaca
    14701 gaaaggaatt gggcttcgct gccctttgcg cggagcgact gagggttcca caccgaatca
    14761 tcgacgttcg cgaaatcggc agcggccttt ccggctccgc gttgaccgac aatatcgacg
    14821 tgcccgaggg ccattacgcc gaagacacga tgaaggtcac ggtggtgccg aaccgaaacg
    14881 ccatcatgtt agccatcgcc ttcggcttcg ccgccgcgcg cagcgctgac gcggtggcga
    14941 tggccgtgca tggtggtgat cgtttcatct atccggactg tcggccagcc ttcatcgacg
    15001 ctttccaggt gatgcaagac cgagcgctcg acggctacgg caacgttcgt ctttatgcgc
    15061 cttatgtgaa tctcacgaag gcggacatag tcgaggatgg agctcgacac ggcacgccat
    15121 ttgcgcagac ctggtcctgc tacaagggcg gcgagcgcca ctgtgggcga tgcgggacat
    15181 gtgtcgagcg gcgcgaggcg tttgatctct ctggccaggc agaccccacc gattacgacg
    15241 acgccgactt ctggctcggc gcgttgcgca ggaggggcgt gtaatgtttc gcatcaccaa
    15301 ggaattccac ttctcggcct cccatcagct cacgtccttg ccgcctgatc accagtgcgc
    15361 ccgcctgcac gggcataact acatcgtcga agtggagctt tcggcaaagg aactgaacga
    15421 gcacggcttt gtgcgcgact atcacgattt ggcgcccctc aaacgctata tcgacgataa
    15481 cttcgaccac cgtcatctca acgacgtact gggacatgag caggtcacgg cggaatgtct
    15541 ggccagacat ttttatgaat ggtgcaaggc acgcctgccc gaaacctcgg ccgtgcgggt
    15601 gagcgaaacg ccgaaaacat gggcggaatg ccggccatga ccgatacgca tcccgcaatc
    15661 cgcgtgagtg agattttcgg gccgacgatc cagggcgagg gcgtgctcat cggtctgccg
    15721 acggtcttcg taagaaccgg cgggtgtgac tatcgctgtt cctggtgcga cagcctgcat
    15781 gcggtggaca accaattccg ccatgaatgg gagatgatgt cctccgacgc ggtgtggcaa
    15841 aacgtggtcg cgctttccgg cggccgcccg ttgatggtct cgctgtcagg cggcaatccg
    15901 gctatccagc ctttcggtgc gctcatcaag cgaggccata atgaaggcta ccgatttgcg
    15961 ctcgaaaccc aaggttccgt ggccagggaa tggtttgccg acctcgacgt gctggtcctc
    16021 agtccaaagc cgccgtcaag cgaaatgacc accgattggg aagctgtcga ggcgtgcctc
    16081 caggcggcac gggacaagcc ggaaatcgtg ctcaagctcg ttgttttcaa cgaaagtgac
    16141 tacgcctacg cgaaagaggc cgccgcgaga tttccgcgac ttccgatcta tctgcaaccg
    16201 ggcaatcata cgccgccacg tcccggaaat gaagacgcgc ctgtcgacct tgacggaata
    16261 acaaaacgta tggagtggct ggttgatagc gtgacccgtg acagatggtt cgaagcccgc
    16321 gtgttgccac agctgcacgt tatgctctgg ggtaacaaaa gaggcgtcta accagcgccg
    16381 actggggcga cggctcgccc cgcgtggcga acggcggtcg cggctgagca gatcgcgtga
    16441 agcccgctca gggactgggg cagggcggaa aagccgtcat agaggaaccg ccagcgggtg
    16501 tccgcgtcga atcaccataa gcccacaact cctgtgccac ggaactgcag ggcaatcgga
    16561 tcaaacgcga tggctcggtt tacgctcaaa atgctcagac tgcagctgag tgcctggccg
    16621 ttccgctttc gaaatattta tcgaacttga ccgcaatgct cctcacgaag ggacggtttt
    16681 ccgccggcac gacgaaactg tctccttgga gttcaagcag tcgagcagga tcctcaaggg
    16741 cgatggagct tgcctgacga aggagccttt ggccggcttt accgaagctc tccaccaggt
    16801 cgatcgccga gaaagcgaag tcgcacatca gtcgctcgat gatccagccc ctcacgcgat
    16861 cgtcttccga aaatgctatg ccacggacgg cggccaactc cccatcttcc accctgcgcc
    16921 gataatcgcc ggtcgaggtc atattctgca cgtagccctg gcgaaaccgg ccgatggatg
    16981 aagagccaag tccaattatc gtctcgcagc gatcctcggt gtagccttga aaattgcgat
    17041 gcaacgcccc cgcacgggcc gctatcgcca atgcatcgcc cggctttgcg aagtggtcga
    17101 gcccgattga ttcgtagccc ttatcctcga tcgttcgcgc ggcaagtcgc gattgagcaa
    17161 accgctccga aggaccgggg agccatgcct catcgatcat cgtctgatgt tttttgaacc
    17221 aaggcacgtg agcatagccg aacagcgcca tcctgtccgg ttccagggtc agcgcctgct
    17281 ccacggtgga acgcacgctc tcgctgcttt gatgcggcag gccatagagc agatcaagat
    17341 tcacggaatc cactccccgg gaacgaagcc cgtcgacgac tgccttggtc tgcaaaaagc
    17401 tttgctcgcg gttgatcgcc ttttgtactt tcggatcgaa atcctgcacg ccgaggctcg
    17461 cccgcgtcat gccgatttgg gcaagcgcgt cgaggcgggc tccgtccata tcgttcggat
    17521 cgatctcgac gctgatcttg gcatcgtgaa caaagtcgaa attgtcccgc agcgcctttc
    17581 ccaatatcac catatgctgc ggcctcagca tcgtcggcga accgcctccc aaatgaaccg
    17641 cgcgaactcg gcccctgccg ctgacaaggc ggccaaccgt cgcgaactcc gcatgcagcg
    17701 aacgcagata tgcggccacc ggctcatagt gccgcgtgtg tttagtgtgg cacgcgcaga
    17761 accagcagag cctgtcgcaa tagggaatat gcaggtaaag cgatatctcg ccgccgcctt
    17821 cgagcgcctc tagccagccg cggtaaacga cagcatcgat acccgcatgg aaatgcggcg
    17881 cgctcgggta gctggtatag cgcgggacgt tctcgcccag tctggcagca agttccggtc
    17941 gcatgtcgtg gtccacctgt ttgcgttgcc gtggactgtg gactcacagg cgaggcagaa
    18001 ccctgatttc ggtcagtctc cagcacgaca aactgccgca aggatttttc taccgtcgat
    18061 tgttatcgtg ccggcggttg ggccgggcga gcagatcggg agagcgatca aatgacagta
    18121 cggcacgaca ttcaccattc cggcatcccc gtttattgct tgtcttgcca ggctcggcaa
    18181 cgcggcctct gcagcgcgct cgatgcagat cgcttggcca agctcgccaa gacttcgtcg
    18241 cggcacaggg tcgaagcggg cgtccagctg atcggcgata ccgaggccgt aaacagctat
    18301 tcaaacgtgc tctcgggcgt ggtgaagctg acgaagagcc tttccgatgg ccgccagcaa
    18361 atcgtcggcc tccagttcgc gccggatttt ctgggacagc ccttcaaggt gaaaagcgca
    18421 acccatgcag aagcggcaac gactgtctcg ctgtgttcgt ttccgaaggc ggcaatcgaa
    18481 cagatgatca agcaatcacc ggagctcgag catcgtctgc ttaagcagac gctgtacgaa
    18541 ctggacgagg cacgcgaatg gatggtgact ctgggctgca agaccgcgcc tgagaaggta
    18601 gcgagctttc ttcttatgat cgcccgcaat attggctcca gtgttggccc gacggccaca
    18661 tcgacgtgct tcgatctgcc gctgacgcgt gccgacatct ctgatttcct tggaattaca
    18721 aacgagacgg tgagccgcca gctgacccga ctgagaatgg acggggtgat ccggatcgag
    18781 aacaaccgcc atgtcacggt agacaacatg agccggctgg aacagcgttg cggtgggcgc
    18841 gggccgaggc gcaaccgcca cgtcaacaag atctgagcgg ctgatcctgc ctcgcttacc
    18901 gtggcccggg acttgcggga tttcgcctgg gccgcaactc taccgttggc cgccggcttg
    18961 ttgttgtccc ttcaacgatg tgtcgacagg tcttgttcat caacctgcgg cgcaacttct
    19021 gtcgtctcgg cggccatgga cgactccggc ccgtagatca cgtcggtgaa ccgaaagcgc
    19081 ccatgtttga cgaacgattt gcgtcgatct gaccgataca cttccgatgt ccttgatcac
    19141 acgcaggatg caccgccgat cccactcata ccatttggcg catgacaccg cagttcacac
    19201 gggtacagca gactaaagac cacccatgca gagatatttc atctcaagat aatcctcgag
    19261 cccgtggcgt gacccctcac gtccgatgcc cgattgcttc aaaccgccaa acggcgccgc
    19321 ctcagatgac attcgtccgg tgttgatacc gaccatgcca tattcgaggg cttcggccac
    19381 gcgccagacc cgcttgagat tggaagcata gaaataggcg gcaagcccgt agatggtgtc
    19441 gttcgccgcg cgtaccacct cgtcgggatc atcaaagcgg atgattggcg ccagcggtcc
    19501 gaaagtctcc tcttgggcga tctgcatcgt actgatcaca ccggtgatca ccgtgggctc
    19561 gaaaaatgtc ccggactgac cgatccggtt gccgccgcaa cggatcgtag cccctttcaa
    19621 cacagcgtcg ctgacatgag cttcgatttt ccggagcgca tggatgtcga tcagcggacc
    19681 gatcgccacg tccgcttcga agccgtcacc aacacgcaag gcctgcacca gctcggcgaa
    19741 cttttcggca aacacatcat gcacactgga atggacgtaa agccggtttg ccgaaacgca
    19801 ggtctggccg gcatttcgaa acttcgcttg gattgcgccc tcgacggcgg catcgacatc
    19861 cgcatcatcg aagacgataa agggtgcgtt gccgccaagc tcgaagctga ctttcttgat
    19921 ttgtccggag cattgccgca ttaagagccg gccgacctcc gtcgacccgg tgaaactgat
    19981 cttgcggacc ttcggattgg cgcagagttc gcgtccgact ccgtcgcctt ccgacgcata
    20041 gaccaggttg agtacgccgt ccgggaagcc ggccagtcgg gcgagcgcga acatggcgcc
    20101 ggcaacgagc ggcgtttgct cggcgggttt caggacgact gtgcagccgg cggcaagcgc
    20161 cggagagatc ttgcgcgcca ccatcgaagc gggaaagttc cagggtgtga tcgcacccac
    20221 aactccgatc ggctgcttga tcaccagcat ccgacgatcc ttggatggcg ctgaaatggt
    20281 ctcgccatag attcggtttg cttcctcggc ataccattgc agataggccg cggcatgggc
    20341 aatttcggat ttcgcctcgg caagcggctt gcccatctcg gccgtcagga tcgccgcgag
    20401 atcatcgctg tgttcgagga tcagctggtg ccattgccac agggcatcgg agcgctcgcg
    20461 cgcggtgaga cccgcccaac atgctctcgc ggcgtccgcc cgatcgatcg ccacggcgac
    20521 atccgccgtc tccatgtcgg gaagctctgc cagaagctca ccggtggagg ggttgtgaac
    20581 cgcaaagcgc ccggcgccac ttgtggcgct gaggtggggg acggatgcca gat
//
