LOCUS       NZ_AP024109            20633 bp    DNA     circular CON 10-NOV-2023
DEFINITION  Mesorhizobium sp. J8 chromosome, complete genome.
ACCESSION   NZ_AP024109
VERSION     NZ_AP024109.1
KEYWORDS    RefSeq.
SOURCE      Mesorhizobium sp. J8
  ORGANISM  Mesorhizobium sp. J8
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1
  AUTHORS   Kusaba,I., Nakao,T., Maita,H., Sato,S., Chijiiwa,R., Yamada,E.,
            Arima,S., Kojoma,M., Ishimaru,K., Akashi,R. and Suzuki,A.
  TITLE     Mesorhizobium sp. J8 can establish symbiosis with Glycyrrhiza
            uralensis, increasing glycyrrhizin production
  JOURNAL   Plant Biotechnol. 38, 57-66 (2021)
  REMARK    DOI:10.5511/plantbiotechnology.20.1124a
REFERENCE   2  (bases 1 to 6690860)
  AUTHORS   Maita,H. and Sato,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2020) Contact:Shusei Sato Tohoku University,
            Department of Environmental Life Sciences, Graduate School of Life
            Sciences; 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/10/2023 07:29:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,439
            CDSs (total)                      :: 6,376
            Genes (coding)                    :: 6,302
            CDSs (with protein)               :: 6,302
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 48 of 74
            Pseudo Genes (incomplete)         :: 41 of 74
            Pseudo Genes (internal stop)      :: 12 of 74
            Pseudo Genes (multiple problems)  :: 21 of 74
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:17:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1606960
            Orig. end                         :: 1627593
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024109.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20633
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hserlactone"
                     /region_number="6"
                     /rules="Autoind_synth"
                     /tool="antismash"
     cand_cluster    1..20633
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="Autoind_synth"
                     /kind="single"
                     /product="hserlactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20633
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[10000:10633](+)"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10633
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="Autoind_synth"
                     /neighbourhood="10000"
                     /product="hserlactone"
                     /protocluster_number="1"
     gene            1004..2548
                     /locus_tag="MJ8_RS07320"
                     /old_locus_tag="MJ8_14500"
     CDS             1004..2548
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200078.1"
                     /locus_tag="MJ8_RS07320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14500"
                     /product="fumarate hydratase"
                     /protein_id="WP_201413755.1"
                     /transl_table=11
                     /translation="MNRTRSTIAGEDIAKSVADALQYISYYHPTDYIRHLLQAYAREQS
                     LAAKNAIGQILLNSRMAAFGRRPICQDTGLVVVFAKVGMDACIKSAASFADIVNEGVRR
                     AYLDPDNPLRPSIVSDPLGGRVNTQDNTPAVVHVDLVPGNRIEITIAAKGGGSENKARF
                     ATLNPGASVADWVVDTVSTLGSGWCPPGLISIGIGGSAEKAMLLAKEVMNEPIDMSELI
                     SRGPSCAEEALRIELYERINALGIGAQGLGGLTTVVDVKVATYPTHAASKPVALIPQCA
                     ANRHLKFILDGTGPVSFEPPDLSDWPQIGHEDLHTTGVRRVNLDALTKEETASWRCGET
                     LLFSGKMLTGRDAAHKRMVELIDAGKPIPVDLQGRVIYYVGPVRAARGEVVGPAGPTTS
                     SRMDGFTEKILAQTGLYAMVGKAERGPAAIESIVKHRTPYLIAVGGAAYLISKSIKAAR
                     IVAFEDLGMEAIHEFEVQDMPVIMAVDVEGNSIHESGPLEWRKRMAADSIARSIGV"
     misc_feature    complement(2606..2618)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.55"
                     /tool="antismash"
     gene            3020..3520
                     /locus_tag="MJ8_RS07325"
     CDS             3020..3520
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1120: AsnC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200079.1"
                     /locus_tag="MJ8_RS07325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="WP_201413756.1"
                     /transl_table=11
                     /translation="MKSKPDPSQLLDAWDIRILSEIQLDGRISKSELAKRVHLSASACS
                     ERLRLLQAAGIIEGFYARLSPALMGGTISIMVEVVLDRHRLEDQWHFEAAIQQVPEILD
                     CWAIGGRIDYLMRVSSPSMAAYQDFMEGLLQAGLGINQYYSLVVTKPVKANSPIPLSSF
                     KRR"
     misc_feature    3695..3706
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 16.93"
                     /tool="antismash"
     gene            3953..5755
                     /locus_tag="MJ8_RS07330"
                     /old_locus_tag="MJ8_14520"
     CDS             3953..5755
                     /GO_function="GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ThiF"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013094.3"
                     /locus_tag="MJ8_RS07330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14520"
                     /product="ThiF family adenylyltransferase"
                     /protein_id="WP_201413757.1"
                     /sec_met_domain="ThiF (E-value: 2e-33, bitscore: 114.3,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVDPDPSMTTPAGPVPRWEKRYPGRLKYELDALRAAGITPEIDKP
                     ALAAGRLSLTVDWPLDTATTLRLQAVFPDAYPHFRPQVFLLAGLDPPPARHRSPREGNL
                     CLLGRDTRQWTPGWSLCTLLQKQLVDAVRDTGEEDPQGEPAEYWWNNLGSDSGSYCLID
                     SDWNLANASHGTLHLRYIHDQTLKVQAENGTSRSPAVRAYVEEVRDEDGQVLHQWAGPL
                     PADLADAKYQLEVPWVRSKDTILPEPQLGAQLKKLRQEYSWLERARPRNYGPGLKFDLR
                     ALVYPSELAFNETGLGWCFIMLFGHVQGFEPKRKKAPKPLHITALPVFRAGPGDIGWRV
                     PAVASLRDKRILVVGVGAVGAPVAIELARNGCRTLDLVDHDVIEPGNTIRWPLGAAVWG
                     QGKAYALQAFLGRQYPATEVHSHPHFIGIAAQAMSDPGDDDILDTLIKDADLVIDGSAS
                     HGVTTLLDERCRGVGVPLISLSATPTLDGGTVVRHVRKSGCPNCLLHAWHKHEIEPPLG
                     QDAGDEALIQPAGCGERTFTGADYDLQELSLQAVRLAIDTLSVKAAEGSVVQTLSFVDD
                     GQARCPPRWRVEPLPKHPDCRGGH"
     gene            5755..6249
                     /locus_tag="MJ8_RS07335"
                     /old_locus_tag="MJ8_14530"
     CDS             5755..6249
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025819.3"
                     /locus_tag="MJ8_RS07335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14530"
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="WP_201413758.1"
                     /transl_table=11
                     /translation="MFPDVDVWLPDIVAQECAIEADNKFPLETGGTFMGWWSSLDTAMI
                     TAMIGPGPGALHERLRFQPDQQWQLEQIAAHYECSGRRETYLGDWHSHPGASSGALSWT
                     DRGVLRRVITTPEARCALPLMMILWGSSGTWQLTAWRAQTVPRVMLWDKLHLDPVQIRA
                     F"
     gene            7182..8588
                     /locus_tag="MJ8_RS07340"
                     /old_locus_tag="MJ8_14540"
     CDS             7182..8588
                     /GO_function="GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (40-391). E-value: 6.8e-78.
                     Score: 254.0. Matches aSDomain:
                     nrpspksdomains_MJ8_RS07340_Aminotran_3.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913767.1"
                     /locus_tag="MJ8_RS07340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14540"
                     /product="aminotransferase"
                     /protein_id="WP_201415342.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.7e-73, bitscore:
                     245.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLSNLGTEELREKDRSFVFHPSTHLASHSRGETPNRIMAGAEGV
                     YIWDTDGRKSLDGFGGLYCVNMGYGCTEIADAIAKQAHELPFAHVYASQGTEPVARLAE
                     AVVEYFGQNMRRVYFGLSGSDANETNIKLVWYYNNILGRPEKKKIISRNRGYHGSGLVT
                     GSLTGLPFFHKFFDLPLNLVRHTTTPHHYRQARDGESEEAFSKRCADELEALILAEGPE
                     TVAAFIGEPVLGTGGIVPPPKGYWAAIQAVLDKYDILLIADEVVCGFARTGEKFGSHLY
                     GMRPDFVTIAKGVSSAYLPISGSIIGDRVWSVLEEGTEKHGPLGHGWTYSGHTLCAAAA
                     LANIQVLKERNILEHVRDVGPYWLSRMKAELEGHPIVGEVRGVGILSAVELMRDPKARV
                     PFEPELQVGVRAAAALLDNGVIGRAMPHADILGYAPPLIITREEVEIIVDATAKSIETT
                     YRALKAEGAI"
     aSDomain        7302..8354
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MJ8_RS07340_Aminotran_3.1"
                     /evalue="6.80E-78"
                     /label="MJ8_RS07340_Aminotran_3.1"
                     /locus_tag="MJ8_RS07340"
                     /protein_end="391"
                     /protein_start="40"
                     /score="254.0"
                     /tool="antismash"
                     /translation="GAEGVYIWDTDGRKSLDGFGGLYCVNMGYGCTEIADAIAKQAHEL
                     PFAHVYASQGTEPVARLAEAVVEYFGQNMRRVYFGLSGSDANETNIKLVWYYNNILGRP
                     EKKKIISRNRGYHGSGLVTGSLTGLPFFHKFFDLPLNLVRHTTTPHHYRQARDGESEEA
                     FSKRCADELEALILAEGPETVAAFIGEPVLGTGGIVPPPKGYWAAIQAVLDKYDILLIA
                     DEVVCGFARTGEKFGSHLYGMRPDFVTIAKGVSSAYLPISGSIIGDRVWSVLEEGTEKH
                     GPLGHGWTYSGHTLCAAAALANIQVLKERNILEHVRDVGPYWLSRMKAELEGHPIVGEV
                     RGVGILSAVEL"
     misc_feature    complement(8699..8712)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.04"
                     /tool="antismash"
     misc_feature    8699..8712
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.03"
                     /tool="antismash"
     gene            8878..9603
                     /locus_tag="MJ8_RS07345"
                     /old_locus_tag="MJ8_14550"
     CDS             8878..9603
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1197:
                     autoinducer-binding transcriptional regulator,"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913768.1"
                     /locus_tag="MJ8_RS07345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14550"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_201413759.1"
                     /transl_table=11
                     /translation="MQEVFETFLERLTESVDETDFREAFARAAQEYDLLQFAYLSLPPK
                     NSGKPRLISNYPPRWTGHYELNRYHTIDPVILQAQCGGCPFLWGLEPSQTEFLNAERKL
                     FAEAAEFGISCGLTVPIIDKRGCFAAVTFAADGPKPAFVRVTKRYRQILLCMASCFHIY
                     ARRKLSNDRIVNGVQLTAREFECLQWAAKGKSAWATGRILGIARRTVSFHLDNVREKLG
                     VETTRQAIALLGESDSSRH"
     misc_feature    complement(9700..9711)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    9757..9771
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.36"
                     /tool="antismash"
     gene            10001..10633
                     /locus_tag="MJ8_RS07350"
                     /old_locus_tag="MJ8_14560"
     CDS             10001..10633
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hserlactone: Autoind_synth"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838475.1"
                     /locus_tag="MJ8_RS07350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14560"
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="WP_201415343.1"
                     /sec_met_domain="Autoind_synth (E-value: 2.3e-42, bitscore:
                     143.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLLIAPERYDDFADDLHAMHRLRHRVFRERLDWDVRTTGGYETD
                     RFDALKPHYLVLRGDGIDGCVRLLPTTGPTMLRDTFPVLLDGEAAPEDPRIWESSRFAL
                     DLPPTAPKRASGIAVGTYMLFAGMIEFGLSRRLSRIVTVTDLRIERILRRADWPLRRLG
                     DPRTIGNTRAVAGYLDVSATSLDAVRRNGGLTGPVLWSPVLHQAADA"
     gene            10904..11461
                     /locus_tag="MJ8_RS07355"
                     /old_locus_tag="MJ8_14570"
     CDS             10904..11461
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533396.1"
                     /locus_tag="MJ8_RS07355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14570"
                     /product="DUF2285 domain-containing protein"
                     /protein_id="WP_263649643.1"
                     /transl_table=11
                     /translation="MGHDAAVFWSPRRCPQVLPVIAHAAPTGSATPPLMPPPCHATLLI
                     LAEGAQYVLLEHAGRHLQLAVSGASVLRPVLIHTDAIWPAADLRHRLWALECLNVLNAH
                     GRLPKRLFRPEARCRRLRFVLRALDGSLAGASYRDIAEVLLGQARVHADWADPRDHLRD
                     RIRRAVRRGRALMNGGYMSFLV"
     gene            complement(11485..11736)
                     /locus_tag="MJ8_RS07360"
                     /old_locus_tag="MJ8_14580"
     CDS             complement(11485..11736)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863472.1"
                     /locus_tag="MJ8_RS07360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14580"
                     /product="hypothetical protein"
                     /protein_id="WP_201413760.1"
                     /transl_table=11
                     /translation="MKHETVDEVPWSDSLTAYDEANFTVYMRFLDAAADNDSYEEMARL
                     ILGIDPEREPERARNAARSHLDRANWFITTGYKELFAS"
     gene            11876..12091
                     /locus_tag="MJ8_RS07365"
                     /old_locus_tag="MJ8_14590"
     CDS             11876..12091
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800225.1"
                     /locus_tag="MJ8_RS07365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14590"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_201413761.1"
                     /transl_table=11
                     /translation="MDLKEVMAINLRRLRHDQDLTQEELAARAELSMRYVGSIERAQVS
                     ASVSVLGRLATALNVDPCDLIRPRQN"
     gene            12389..12604
                     /locus_tag="MJ8_RS07370"
     CDS             12389..12604
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563594.1"
                     /locus_tag="MJ8_RS07370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_201413762.1"
                     /transl_table=11
                     /translation="MDLKEVMAINLRRVRHDQDLTQEELAARAELSMRYVGSIERARVS
                     ASVSVLGRLAAALNVDPCDLIRPTQK"
     gene            complement(13075..14412)
                     /locus_tag="MJ8_RS07375"
                     /old_locus_tag="MJ8_14600"
     CDS             complement(13075..14412)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199513.1"
                     /locus_tag="MJ8_RS07375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14600"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_201413763.1"
                     /sec_met_domain="DAO (E-value: 1.5e-38, bitscore: 131.9,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKTFRARRLPAHGVEPGWAALLPPRTPTPELDRAITADVVVIGA
                     GFAGLAAATRLTELDPGLSVAVIDADVVGNGASGRNSGFLIDLPHDISSGNFGVDAATK
                     SRNEIMVARSAIQLYARLANTNGWDKDVFDPSGKYSVAITDAGKEHLTTYSSKLKALGE
                     PHQLLDAAAIADVTGTRSFKSGLFTPGAVMIQPVALVRGIADFFEGSSVKIFERTPALS
                     IETSPTGCIVKTPKGEIRADKVILATNGHAESFGFGSGELLHVFTYASLTEPFDPTVLG
                     GLRKWGATPAAPMGTTVRRINGAGGDRILIRSRYSYIPSIEVGASSMKGASKLHDRKFT
                     DRFPELRHLKFQFRWAGAMALTWNTVPLFGELEDRIFCACVCNGIGGTKATAAGIATAD
                     LVVGHRSALGEIFQAFDKPQKLPPRPLVEVGARFNLALREWRAGRE"
     gene            complement(14415..15302)
                     /locus_tag="MJ8_RS07380"
                     /old_locus_tag="MJ8_14610"
     CDS             complement(14415..15302)
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017957287.1"
                     /locus_tag="MJ8_RS07380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14610"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_201413764.1"
                     /transl_table=11
                     /translation="MSTFAFPGLNLAITTPFNASGHVDFGRLEDLIERYLAAGVDGFVF
                     SSGTGMHVYLTPEESKSVIERGARVVAGRAKVIAQTSALLTADVVARTQSAKDVGADGV
                     MILPPFFEGPSDDGSIFAFYADVAKVGLPIIGYNVPQAVGVRITPSLLRMLCDLPEFQA
                     VKDSSGDLISQVRLIGTGLGVINGADPLVPFSLLAGCSGLIWGGANIAPRTCVAITRAA
                     REKRWDDVQSIWRKLEPLMSLLWEGDYVQHVYAAAELTGYGCGVARKPFGSLSSDRIAA
                     LRNALAGLVAQETL"
     gene            complement(15304..16788)
                     /locus_tag="MJ8_RS07385"
                     /old_locus_tag="MJ8_14620"
     CDS             complement(15304..16788)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240428.1"
                     /locus_tag="MJ8_RS07385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14620"
                     /product="aldehyde dehydrogenase"
                     /protein_id="WP_201413765.1"
                     /sec_met_domain="Aldedh (E-value: 1.3e-158, bitscore:
                     526.9, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHGTVDYKTQAQSIRLPTSMIVNGKPVAAISGKTYPTINPATGKV
                     LADLPSGSAADVDRAVAAARQAFEDGRWSKLTPANRKQALLRLASVIEENALELALMES
                     IDSGKTITDCINIDVPEAINSLRWHAEAVDKIYDQVSPANDSSLGLIVREPIGVVGIVL
                     PWNFPLLMLAWKIGPALAAGCTVVVKPAEETSLTTLRVAELALDAGIPAGVFNVVTGDG
                     PDVGEPVGRHADIDAVSFTGSTETGRRFLKYSSESNLKEVVLEMGGKNPCIVMDDVRNL
                     DAVASHVCNGAFWNVGQNCSAISRLFVHRAVKDRLLQKLAEVAKTWIVGDPLAPETRIG
                     PLVSTVHFEKVSGYLENDGKIVAGGNIVGGTTIEPTIIEFSRHWGKHIREEIFGPILSV
                     IPVDSLAEAVSLANQTEYGLAASVFTGDGTRALRAARAIRAGTVTVNCYGEGDAATPFG
                     GYKQSGFGGRDKSIHAHDQYCQTKTLWVDLSSDEGI"
     misc_feature    complement(17707..17721)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.72"
                     /tool="antismash"
     gene            17712..18689
                     /gene="proX"
                     /locus_tag="MJ8_RS07390"
                     /old_locus_tag="MJ8_14630"
     CDS             17712..18689
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="proX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331056.1"
                     /locus_tag="MJ8_RS07390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14630"
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="WP_201413766.1"
                     /transl_table=11
                     /translation="MKFKIAVAALAIMASGTLSAAAEQVKYGWIGGVNEELFQGYVVRD
                     GLRQLGFDVEEQPEQVQVTLAYTAVASGDLQFYPAFWTPLHDAFWKGAGGADKLMPVGT
                     LVHGDIQGYLIDKKTADATGIKYLEDLKDPEKAKLFDIDGNGKADLFGCDPGWGCERMI
                     EHHLDAYGLRDTVEHKQGGYFAIMADAIQRIKDGKPTLYYTWIPGWISGVLLPGKDVTW
                     LNVKTTSLPDGQTGSTEIKGLGNLGFPVNDTKVMANTEWLKANPSAKKFLEELSIPVKD
                     VTAENKLIYEGEKNPDQIAKHATDWIAAHKAQWDKWIADAKAAK"
     gene            18900..19673
                     /locus_tag="MJ8_RS07395"
                     /old_locus_tag="MJ8_14640"
     CDS             18900..19673
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (14-116). E-value: 1.1e-09.
                     Score: 30.4. Matches aSDomain:
                     nrpspksdomains_MJ8_RS07395_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856577.1"
                     /locus_tag="MJ8_RS07395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_14640"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_201413767.1"
                     /sec_met_domain="adh_short_C2 (E-value: 4.7e-30, bitscore:
                     103.9, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNYLDRFSLAGKRAFVTGGARGIGLEICRALGQAGAAIVLADLGE
                     EAARSAAEGLEREGLEVCYRIVDVTKSADVTALAEELNAQRAVDILVNNAGVGPKAEAL
                     DTLDEVWSKTMNVNSDAPFWCSRAFGRHMTARGSGNIVNIGSMCGLIVTQPQNNTCYIA
                     SKGALHMMTKSLACAWAKFNVRVNAVAPGYIATDMLDRFKTDNAVWYDRWIEQTPLGRM
                     GTPQEIASAVLYLASEASSYCTGTILSADGGYTSL"
     misc_feature    complement(18932..18945)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.53"
                     /tool="antismash"
     aSDomain        18942..19247
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MJ8_RS07395_PKS_KR.1"
                     /evalue="1.10E-09"
                     /label="MJ8_RS07395_PKS_KR.1"
                     /locus_tag="MJ8_RS07395"
                     /protein_end="116"
                     /protein_start="14"
                     /score="30.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="FVTGGARGIGLEICRALGQAGAAIVLADLGEEAARSAAEGLEREG
                     LEVCYRIVDVTKSADVTALAEELNAQRAVDILVNNAGVGPKAEALDTLDEVWSKTMN"
     CDS_motif       18945..19019
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MJ8_RS07395_0001"
                     /evalue="1.50E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="MJ8_RS07395"
                     /protein_end="40"
                     /protein_start="15"
                     /score="20.9"
                     /tool="antismash"
                     /translation="VTGGARGIGLEICRALGQAGAAIVL"
     misc_feature    complement(20154..20170)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.88"
                     /tool="antismash"
ORIGIN
        1 gccttggaac tatcccgccg ttctcatcgc ccgcaagctc gcccctgctt tggcagcagg
       61 ctgcccggtg atcctcaaag gggctgaaga gacacctagc gccgcagttc acatcgtcga
      121 agccttgcaa caagcgggta ttccggacgg cgtggtcaat ctcgtctttg gcgtgccggt
      181 gcaaatatcc cgccatctcc tcagctcgcc gatcgtgaag gtcctaacgt tcacgggctc
      241 caccgaggtt ggaaaacagc ttgccaagct cgccgccaat aacttgcagc gatgcatcct
      301 cgagctcggc gggcactccc cggtcgtcgt ctgcgaggat gccgacctgg ccacggccat
      361 accggcaatt tcggattata agttcgagtg cgctggccag agttgcaatg cgcccagcag
      421 gattctcgtc gcgcggcctc tttatgggca attcctgtcc cggatgaccg aagtcgctcg
      481 caaggtcaag atcggcccac cggacgatcc ggcgacggag atgggtccaa tggcaaacgg
      541 acggcgaatc gaggctatgg agcgcctggt ccaggatgcg gtcgagcgcg gcgccaccgt
      601 cgaaaccggt ggcacccgcc tgggccaacc gggtttttac tggcctccaa ccatcctgac
      661 cggcgtgcca aaggatgcca gagtgcttca tgatgagccg ttcggaccga tcctcaccgt
      721 ggcgccgttc gacacgatcg aggaagcaat cgaagaagcc aacgccacgg actatggctt
      781 ggccgcctac ttcttcaccg gcgctgacga tatcaaacgg gcgctgatcg gcggtctttc
      841 ggccggcgct cttagcgtaa attatctcaa aggcgtgtct gcggatgcgc cgtatggagg
      901 cgttaagcag agcgggtatg gatacgaggg aggcgagcag ggggtgcgga gcttccaaag
      961 cctgaagctg gtcaacgggt tcggttcatt cggatgagat ttcgtgaata ggacaagatc
     1021 gaccattgcc ggggaggata tcgcaaagag cgtcgcagac gcccttcagt acatttcgta
     1081 ttaccatccc acggactaca tccggcacct tttgcaggcg tatgcgcgcg agcaaagcct
     1141 ggcggcaaag aacgccatcg gacagatctt gctcaactct cgcatggcgg ccttcgggcg
     1201 gcgcccgatc tgccaggaca ctggcctcgt agttgtcttt gccaaggtgg gcatggacgc
     1261 ctgcatcaaa tcagcggcca gtttcgctga catcgtgaat gaaggcgtcc gccgggcata
     1321 tcttgacccg gacaacccgc ttaggccgtc gatcgtctcg gatccgcttg gcgggcgggt
     1381 gaacacgcaa gacaacacgc cggcagtcgt ccacgtcgac ctcgtgcctg gcaaccgcat
     1441 tgagatcacc atcgcggcaa aaggcggcgg atccgaaaac aaggcgcgct tcgccaccct
     1501 gaatcccggt gcgtccgttg ccgactgggt ggtcgacacc gtttcgacgc tgggcagcgg
     1561 atggtgccca cctggcctga tctccatcgg aatcggtggc agcgctgaaa aggcgatgct
     1621 gcttgccaag gaggtcatga acgagccgat cgacatgtcg gaattgatat cgagggggcc
     1681 atcatgtgcg gaggaggcac tgcggatcga gctttatgaa agaattaacg cgctgggcat
     1741 tggggctcag ggcctaggcg gcctgaccac ggtcgttgac gttaaggttg cgacctatcc
     1801 cactcacgcc gcctccaagc ccgtcgcgct cattccgcaa tgcgcggcca accggcacct
     1861 gaagtttatc ttggacggca ctggacccgt aagttttgag ccgcctgacc tttccgattg
     1921 gccccagatc ggtcatgagg acttgcacac aaccggcgtc cgccgggtca atctcgatgc
     1981 gctcacgaag gaagagacgg catcgtggcg ctgcggggaa acccttcttt tttccgggaa
     2041 gatgctgacg ggccgtgacg ccgcccacaa gcgaatggtc gagctgatcg atgccggcaa
     2101 gccgatcccg gtcgatctgc agggacgcgt gatctactac gtcggacctg tccgcgcggc
     2161 gaggggtgag gtcgtcgggc cggccggccc aacaacctcc agccgtatgg acggttttac
     2221 tgaaaagata ctcgcccaga ctgggctata tgcgatggtc ggcaaagccg aaaggggacc
     2281 ggccgctatc gagtcgatcg tcaaacacag gacgccctat ctcatcgcag tgggtggtgc
     2341 agcctatctg atttcaaaat cgatcaaggc ggcgcggatc gtagcctttg aagacctggg
     2401 catggaagcc atccatgaat tcgaggtcca agacatgccg gtcatcatgg ctgtcgatgt
     2461 cgagggaaac tccattcacg agtctgggcc gcttgagtgg cgtaagcgca tggcggccga
     2521 cagcatcgcc cgcagcatcg gcgtttgagt tcttccgggg ctgcgcgttc gccgagactc
     2581 tcgcgccgaa ttggccgcga tggtgatgca aactgcattc tggagttcag ctcctcggca
     2641 agagcggcct tcaagtcgcc gctcgtcgcg aaaatgcccg gatgttaaga gtggttcgac
     2701 agatggtcat gcgaattgat gcctggtgct gtcaggagcc aatcagccaa gcaccgccag
     2761 tgccgacaaa agccccatcc atttacgccc agatcggtgc cgacagccga tcctcgcact
     2821 gtgccttgtc gcccgcgcaa gcgagggcaa tgcccaaccg ttctgcaccc cttctgattg
     2881 gatccgcgat cagtcagatg acgtacatca acttcacact gacgttgtcg gcccggagac
     2941 agaattcccg ccaaattcgc ttgcttcgat tgatataaag tttcggcgac caatcgacaa
     3001 cacttgagag caacagatca tgaagtcgaa gcctgatccc tcgcagctgt tagatgcgtg
     3061 ggatatccgg attctttcag aaatccagtt ggatggtcga atttcaaaaa gcgagttggc
     3121 aaagcgtgtt cacctttcgg cgtcggcctg ttcggagcgc ctccgcctac tccaagccgc
     3181 gggcatcatt gagggattct atgcgcgact gagtccagcc ctcatggggg gcactatctc
     3241 tataatggtt gaggtggttt tggaccgcca ccgtctcgag gaccagtggc acttcgaggc
     3301 tgcgatccaa caagttccgg aaatcctgga ctgctgggct atcggggggc ggatcgacta
     3361 tctgatgcgc gtttcatctc cttccatggc tgcgtatcag gatttcatgg agggattgct
     3421 gcaggcaggc ttgggtatca accaatacta cagccttgtc gtgacgaagc cagtgaaggc
     3481 caattcacca attccgttgt cctccttcaa gcggagatga gcctctggtt gaattcgtcg
     3541 gtaattagtc tcgattccgc gaattcatcg gtcaaatacg cacatttccg cgaaattcga
     3601 ttggccggtg cgcatataag gagccagtcg accgagtgtt ccgccacagt ttttcgggcg
     3661 gaaagcaacg aagcgtctga gcagtctagt cggagaaaaa gttctcgccc aggatttaaa
     3721 tctcagaagc acatcattgc cacgaaccgt tgaccggcaa ccctgcgaca agctcacaca
     3781 gcgagaatat ggagaccgac tatggctgga agacccgacg accccggaaa acgaccgcca
     3841 gataatcccg gaaaaggacc gcctcacgaa gtgcctcctg ggccgcctca cggtgttccg
     3901 ccgggtccgc ctgatccgcc tgcacccccg ccgcgcaagg tgggatgaga ggatggttga
     3961 tcccgatcct tccatgacga ctccggcggg gccagttccc cgctgggaga agcgttatcc
     4021 ggggcggctc aaatacgaac tcgacgcgct gcgcgctgcc gggataacgc ctgagattga
     4081 caagccggcc ctcgctgccg ggcgattgtc tctcacagtc gactggccgc tcgatacggc
     4141 gaccaccctt cggctgcagg ctgtctttcc cgacgcctac ccgcactttc gcccccaggt
     4201 ttttctgctc gccggccttg atcccccgcc tgcgcgccac cgcagtcctc gcgagggcaa
     4261 tctctgcctg ctcggccgcg acacgcggca gtggacaccg ggctggtcgc tttgcacgtt
     4321 gctgcaaaaa cagctcgtcg atgccgtgcg tgacaccggt gaggaagacc cgcagggcga
     4381 gcctgccgaa tactggtgga ataaccttgg cagcgattcc ggatcttatt gtctgatcga
     4441 cagtgactgg aatctcgcca atgcgtcgca tggcaccctg catctgcgtt acatccatga
     4501 tcagacgctc aaagtgcagg ccgaaaacgg tacaagccgc agtccggcgg tcagggccta
     4561 tgtcgaagag gttcgcgatg aggatggcca agtccttcac caatgggcgg gtccgctccc
     4621 cgccgatctg gccgacgcaa agtaccaact cgaagtcccg tgggtaagat caaaagacac
     4681 gatcctgccc gaacctcagc tcggcgcaca gctgaaaaag ctccggcaag aatactcatg
     4741 gctcgaacgt gcccggccgc gaaattacgg tccgggtctt aagttcgatc tccgcgcgct
     4801 tgtctatccg agcgagcttg cctttaacga aacgggcctc ggctggtgct tcatcatgct
     4861 cttcggtcat gtccaaggct ttgagccgaa gaggaaaaaa gcccccaaac cgctgcacat
     4921 caccgcgttg cccgtttttc gcgccggacc aggcgacatt ggctggcgtg ttccggccgt
     4981 tgcctccctc agagacaagc ggatccttgt cgtcggcgtt ggcgccgtcg gcgcaccggt
     5041 ggcgatcgaa ctggcccgca acggatgccg cacgcttgat ctcgtcgatc atgacgttat
     5101 cgagccggga aacacgatcc gttggccgct gggtgcagcc gtctggggac agggcaaggc
     5161 atacgcgttg caggcctttc ttggccgcca gtatccggca actgaagtcc atagccatcc
     5221 gcatttcata ggtattgcgg cgcaggctat gagcgatccg ggtgacgacg atatcctcga
     5281 cacgctcatc aaggacgccg atctcgttat cgatggcagc gcttcgcatg gcgttacaac
     5341 gctgctcgat gagcgctgcc gaggcgtagg cgttcccctc ataagcttgt ccgctacgcc
     5401 gacgctggac ggcggcaccg tggtccggca cgtccgcaaa agcggctgcc cgaactgcct
     5461 tttgcacgca tggcacaagc acgagatcga acctccgcta ggacaggatg ccggtgatga
     5521 ggccctgatc cagccggcag ggtgcggaga gcgcacattc accggcgctg actatgatct
     5581 gcaggaactt tcgttgcagg cggtgaggct ggccatcgat accctctcgg ttaaggcggc
     5641 cgagggttcg gtcgtgcaga cactcagctt tgttgacgac ggacaggcgc gctgtccgcc
     5701 gcgctggcgt gtcgaaccgc tgccaaaaca tccggactgc cggggcgggc attgatgttt
     5761 ccggatgtcg acgtctggct gcctgacatc gtggctcagg aatgtgcgat tgaagccgac
     5821 aacaagttcc cgttagagac gggcggcact ttcatgggct ggtggtcgag tcttgatacg
     5881 gccatgatca ccgccatgat tggtccggga cctggcgcgc ttcacgagcg cttgcgcttc
     5941 cagccggatc agcaatggca actcgagcag attgctgccc actatgagtg ctctggccgc
     6001 cgcgagactt acctcggcga ctggcatagc caccccggcg cttcgagcgg cgcccttagt
     6061 tggaccgatc gcggtgtcct ccgccgcgtt atcaccacgc cagaagcgcg ctgcgcactg
     6121 ccactgatga tgatcctatg gggttcttcc gggacatggc aactgacagc gtggcgagcg
     6181 caaacggtgc cgcgtgtgat gctctgggac aagctgcatc tcgatccggt gcagataagg
     6241 gctttttaga agctacgcat acgcaggcag atctcagcgg cggcttggca tccgcttcgt
     6301 tcacgcgacg tctggcgggg gacgcgtgga gcggtccgcc cgatgtcttg tgctgaagca
     6361 ggttgaagtc gtcgaaacga ccgcgcccgc cggcgtggcg ccgtagcatt ggctgctcct
     6421 gaccccctat gcggaaccag ctccgcccaa gcctggcaga tcgtctcctg tactacgagc
     6481 gcgggctcga gccgttctcc cgatcgataa agatccaggg cttgaagagc aagaagggca
     6541 atcaggacag atggaagcgt ctctgccgtg tgaacaccag ggtcgtgcct tggcaagccc
     6601 acccaaatct atacgaaaaa tctaaagcac gtcctgaaat cgtaagtcga tcgtgtgtga
     6661 agaggggacc tttgctgccc gatgaggaga ctgcgggcgg tctacttgag catggttccc
     6721 ctgccgcgcg actctgcgat cccaagcctc cgtaaccctg cgaatctgct ttaagagtag
     6781 tggcaatcct gaatagccca gggcgtgcgc cacgagcgtg cgcgtcacca tgccgccgaa
     6841 catgttctgg cgagacgacg aacacatccg gaagttccgt ttggctgctg gttggggcct
     6901 tcggacagcg tctctcgtaa catagtgacc caggggaaag ccgtaccttc gcagccatcc
     6961 gccgagaaca gacggcggcg gccgcaatga tccccttatc cgcctcaaag agaaggtaag
     7021 ctccagaacc ttggttttca ctgcgttcga ggccgatttt cgcgaattcg tcggctaaag
     7081 aggcgcattc ccgcgaaact cgaaaggtgt ttgcggatat cttgttttca attggcggct
     7141 gggtgggtgt cttctgcctg ctgctggcga ggagaggaac catgagcttg agcaatttgg
     7201 ggactgagga actgcgggag aaagatcgaa gcttcgtctt tcatccgtct acgcacttgg
     7261 ccagtcacag ccgcggcgag acgccaaacc ggatcatggc cggtgcagaa ggcgtgtaca
     7321 tctgggatac tgacgggcga aagagccttg acggtttcgg tggtctctat tgcgtcaaca
     7381 tgggatatgg atgcacggag atcgctgacg cgattgccaa gcaagcccat gaactgccgt
     7441 tcgcacatgt ctatgccagc caaggcactg agcccgttgc ccgtttggcg gaggccgttg
     7501 ttgaatattt cggtcaaaat atgcgaaggg tctatttcgg cctttcggga tcagatgcca
     7561 acgagaccaa tatcaagctg gtctggtact ataacaacat cctcgggcgc cctgaaaaga
     7621 aaaagattat ctctcgaaat cgcggctatc acggttccgg gctggtaacg ggaagcctga
     7681 ccggccttcc gttctttcac aaattcttcg accttccgct caatctggtg cgtcatacga
     7741 caacgccaca ccactatcgc caagcgcggg acggcgagag cgaagaagcc ttctccaagc
     7801 gctgcgccga cgaacttgaa gccctgatcc ttgccgaggg gccagagacg gttgcggcct
     7861 tcatagggga gccggtactc ggaaccggcg gcatcgttcc gcctcccaag ggatattggg
     7921 cggctattca ggcggtgctc gacaaatatg acatcttgct gatcgcggat gaggtcgtct
     7981 gcggattcgc gcggacaggc gagaaattcg gatctcacct ttacggcatg cgtccggact
     8041 tcgtcacgat agcgaagggt gtgagttcag cctacctgcc catctccggt tcgatcatcg
     8101 gggatcgcgt ctggtcggtc ttggaagaag gaacggaaaa gcacggcccg ctgggccatg
     8161 gctggacata ttctgggcac acgctctgcg cggcggccgc ccttgccaac atccaagtac
     8221 ttaaggaaag aaacatcctg gagcatgtcc gggatgtcgg cccgtactgg ctttcgcgta
     8281 tgaaggcgga actggaggga catcctattg tcggcgaagt tcgcggtgtc ggaattctgt
     8341 cggccgtgga gttgatgcga gatcccaaag cgcgcgtgcc gttcgagccc gaacttcagg
     8401 tcggcgtgcg ggccgctgct gccctgcttg acaatggtgt catcggccgc gccatgccac
     8461 atgcggatat cctaggatat gcgccacctc tcatcatcac cagggaggag gtggagataa
     8521 tcgtcgacgc gacggcgaaa tcgatcgaaa ccacctaccg ggcactcaaa gctgagggcg
     8581 caatatgatc ctggcggtgg ctgtcggacc ggcaagacca cgatcacgac agtaggccaa
     8641 tcggctcaat aagatgccgg cgtcgacttg gggccgacac gtaagggtag gtcggcgaca
     8701 aacgtttttt cgcagctccg ccgcggctgt gccgatctcc ctggatcgct ccctttccgc
     8761 ggcgagccct gccggtttcc tcccgaccgg cagggcgcct catgccagcg ttgccatgct
     8821 ggcgtgtttg tctcattcaa tcgacacaac cccgaaaggg gggagggtga aatctgaatg
     8881 caggaggttt tcgagacatt cctggagcgg ctgactgaaa gtgtcgatga gaccgacttc
     8941 cgcgaagcct tcgcccgtgc agcgcaggaa tacgatcttc ttcaatttgc ctacctttcg
     9001 ttgccaccaa aaaactccgg taagccaagg ctcatctcga actatccgcc ccgctggact
     9061 ggccattatg agctaaaccg ctatcacacg atcgatccgg tcatcctgca ggcgcagtgc
     9121 ggcggatgcc cgtttctttg gggtcttgag cccagccaaa ccgaatttct gaatgccgag
     9181 cggaagctct tcgcagaggc ggccgaattc ggcataagct gcggcttgac ggttccgatc
     9241 atcgataagc gaggctgctt cgcggcagtg actttcgccg cggacgggcc caaaccagcc
     9301 tttgtgcgcg tcacaaagcg ctaccggcag atattgctgt gcatggcgag ctgctttcac
     9361 atttatgcgc gccgcaagct ttcgaacgat cggatcgtca atggggtgca attgacggcc
     9421 cgcgaattcg agtgccttca gtgggctgcg aagggcaagt ctgcctgggc gacaggccgc
     9481 atacttggga tcgcacgacg aaccgtctcc tttcacctcg acaatgtccg cgaaaagctc
     9541 ggagtggaaa caaccaggca agccatcgcg ctgctgggcg aatcggattc ttcgcgtcac
     9601 tgatgcgtcg ctgttagtag tgccgccgca caggctgtct tggcgaggct gtacgcctcc
     9661 agcgctattc actaggcgcc attcttcggt cagaggcctg ctttcatttc cgttcccgtg
     9721 acggctcggc gaggaccggg ctgggactga ttcatcgtct catcgtgaaa aatgcctgct
     9781 gtgtcggcac ggaacatccg cccgacaatc gggtgcaccg ggcatagctc ttggccgttg
     9841 gctacgcgga ctctccctcg agaaagccgg ccgcccgcgc cgttatgcag cttccgaaga
     9901 gccaaccaaa caacattcta cctgtgcgac tgcacaggct gtttcgactt ccgtcttggt
     9961 ctccaatcgc agggtcattc ttctcgacga ggcccatatg atcctgctgc tgattgcacc
    10021 cgaacggtac gacgatttcg ccgatgacct gcatgccatg caccgtctgc ggcatcgcgt
    10081 cttcagggag cggcttgact gggacgtgcg gaccaccggc gggtatgaga ccgaccgttt
    10141 cgatgcgctc aagccccact acctggtgct acgcggcgat ggcattgacg gctgcgttcg
    10201 cctgttgccg acgaccggac cgaccatgct tcgcgacacg tttccagttc tgctggacgg
    10261 agaggcggcg cctgaagatc ctcgcatttg ggagagcagc cgcttcgcgc tcgacttgcc
    10321 gccgaccgca ccaaagcggg cgagcgggat tgccgtgggc acttacatgc tcttcgccgg
    10381 catgatcgag ttcggactgt cgcgccgcct cagccggatt gtcaccgtca cggatttgcg
    10441 catcgagcgc atccttcggc gagccgactg gcctctgcga cgcctcggcg atccacgcac
    10501 gatcggcaac acgcgcgccg tcgccggata tctcgatgtc tccgcaacga gcctggacgc
    10561 cgtgcggcgc aatggcggcc ttaccggccc ggttctctgg tccccggtgc tgcaccaggc
    10621 tgctgatgct tgatggccag ctcaatgcgt gggaagcggg tcgtcagttt cgaagagcgc
    10681 aacgccactt ggccggattg gtgggatgaa gcgccatacg accacaccgt tgagcttacc
    10741 cggcggggtt gggcatggga attcttgcga cgcaatccgg catttcagca tgatctcgct
    10801 catgctctga atcatgccag ctatacggga acacggccat cgctcgcaat aatcgaatcg
    10861 ggcgtcgatc tgtcgcgctg gggggcgctg tttcgcaagt tcattgggac acgacgcggc
    10921 cgtcttctgg tcgccgcgtc gttgcccaca agtcctgccg gtcatcgccc atgcggcacc
    10981 cacggggtcg gcgacgccgc cattgatgcc accaccatgc catgcgacgc tgctgatcct
    11041 ggctgaaggt gctcagtacg tcctgctcga acacgccggc cggcatctgc aactcgccgt
    11101 ttccggggcg agtgtcctcc gcccggtgct catccacacc gacgcgatct ggccggccgc
    11161 cgatttgcgg catcgcctct gggcgctcga atgcctaaac gtgctcaacg ctcatggtcg
    11221 attgccgaaa cggttgttcc ggccagaggc gcggtgccgg cgcctgcgct tcgttcttcg
    11281 cgccctcgac ggttcgctcg ctggagcctc ctatcgcgac attgccgagg tgctgctcgg
    11341 tcaggcgcgc gtccacgccg actgggctga tcctcgggac cacctgcgtg accgcatccg
    11401 tcgcgccgtt cgccgcggca gggcgctcat gaacggcggc tacatgagct tcctcgttta
    11461 attaagtgct gaattagatg aacgtcagct ggcaaacagt tccttgtagc cggtcgtaat
    11521 gaaccagttc gcccggtcga ggtggcttct agccgcgttt cgggcgcgtt ccggttcgcg
    11581 ctcgggatcg atgcctagga tcagccgtgc catttcctcg tagctgtcgt tgtcggcggc
    11641 cgcgtccaga aaccgcatgt agaccgtgaa atttgcctcg tcgtaagccg tgaggctgtc
    11701 cgaccaggga acttcgtcga ctgtttcatg cttcatggcg caccttcgaa cattgtcatc
    11761 cagcgaggcg ggacgccttg tcagaggcac ctcacaagct gctctcgagc gcacatatag
    11821 cgaataggaa cgatcattcc tagaacgata gttcctgttc tgtccgtatc gcggcatgga
    11881 tcttaaggag gtcatggcga tcaacttgcg tcgtttgcgc catgatcagg acctgacgca
    11941 ggaagagctg gcagctcgcg ccgagcttag tatgcgttac gtgggctcaa tcgaacgcgc
    12001 ccaggtttcg gcgagcgtca gtgtgctcgg ccggctggcc accgcgttga acgtcgatcc
    12061 ctgtgacttg atcagaccac ggcagaacta gaagcaatgc tgcttgcatc cggcagatcg
    12121 attcaaagcc gcccggtact cgtgtgcgtc gttatgggcg gcagcgtcca gtcatgtaga
    12181 tcgtggaatt tgcctggtgg tacgctgtcc gacaagggaa ctttgtcgac tggtctcatg
    12241 cttgacggcg gacctttgaa catcgtcatc gagcgcaaag cgggagcggc tttgtcataa
    12301 gcgcgccgaa ggcgctccct cgaccccgat atgcgaatag gaacgatcat tcctagagcg
    12361 ttctttgtcg ttctgtgcgt atcgcgccat ggatctcaag gaggtcatgg cgatcaactt
    12421 gcgtcgtgtg cgccatgatc aggacctgac gcaggaagaa ctggcagccc gtgctgagct
    12481 cagcatgcgt tacgtgggct caatcgaacg cgcccgtgtt tccgccagcg ttagcgtgct
    12541 cggacggttg gctgctgcgt taaacgtcga tccctgcgac ttgatcagac cgacgcaaaa
    12601 ataaagaatt gttcctattc gctttctcga atcgtcatcc gcgaacttgg gccgtctctc
    12661 cgccagatcg gccgcctaca ggtgggctga tctctaggct cacggtggaa atgcgtgacg
    12721 caccagtcgt ggtctgcctt gacagccgtc cacccaggtc aatgtgttcg agttaaacat
    12781 tggactgtct tcccgccatt tgagcgagcg ggacatggaa acgtcgaggg ttagcggctc
    12841 acctgagatg cccacgcaag ctcgttgcgc tggtcattga gaacatcaga aagatcaaga
    12901 acggctcgat ctcatgctcg ctcaggacgt ggggcgttcg cgacggctag ccgcggccac
    12961 gcaaccatat caaagtgccc gccactcccg cacgcgccac aacgtgccgc cttcatcggg
    13021 cgaaacatag aaggcgcaga cataaggagc ctggccaaga tgacagtgcc gatgctattc
    13081 gcgcccggcc cgccactccc gcagtgccag gttgaaccga gcccccactt cgacaagcgg
    13141 ccttggcggc aacttttgcg gcttgtcgaa tgcttggaaa atttccccga gcgcagaccg
    13201 gtgaccgacg acgaggtccg cagttgcgat gcccgcggcc gtggccttgg tgcctcctat
    13261 gccgttgcac acgcaggcgc agaaaatacg gtcttccaac tcgccgaaca gcggcaccgt
    13321 gttccaggtg agggccatgg cgcccgccca gcggaactgg aatttcagat gtcgaagttc
    13381 aggaaagcgg tcggtgaact tgcgatcgtg aagtttgctc gcgcctttca tcgagctcgc
    13441 accaacttcg atgctgggga tgtagctata acgcgatcgg atgaggatcc tgtcccctcc
    13501 agccccgttg atccgtctca ccgtcgtgcc catcggcgct gccggcgttg ctccccattt
    13561 cctcagcccg cccaggaccg ttggatcaaa cggctcggtc aacgatgcgt aagtgaagac
    13621 atgtagaagt tctccgcttc cgaaaccgaa gctttcagca tggccgttcg tcgccaggat
    13681 caccttgtcg gctctgatct cgccctttgg cgtcttcaca atgcagccgg tcggggaggt
    13741 ttcgatggac agtgcgggcg tccgttcgaa tatcttgacg gacgatcctt caaagaagtc
    13801 ggcgatgccc ctcaccagcg caaccggttg gatcatgaca gcacctggag taaagaggcc
    13861 cgatttgaac gaacgcgtgc ccgtcacgtc ggcgatcgcg gccgcgtcaa ggagctgatg
    13921 cggttcgccc aatgccttca gcttggaaga atatgtcgtc agatgttcct taccggcatc
    13981 ggttatggcc acgctgtact tgccgctggg atcgaagaca tccttgtccc agccattcgt
    14041 gtttgccagc cgcgcgtaga gctggatggc gcttcgcgcc accattatct cgttacggct
    14101 cttagtggcg gcgtctaccc cgaaattgcc cgacgatatg tcgtgcggta ggtcgataag
    14161 gaaaccggag ttacggccac tggcaccatt gccgacgaca tcagcatcga tgaccgccac
    14221 ggaaaggccc ggatcgagct cagtcagtct tgtcgccgcg gccaatccgg cgaagccggc
    14281 gccgataacg accacatcgg ccgtgatggc gcgatcgagc tcaggcgtcg gcgtcctcgg
    14341 aggaagcagc gccgcccagc ctggttccac accatgcgcc ggcagccggc gggcgcgaaa
    14401 agtcttggtc atcgtcacaa ggtttcttgc gcaaccagac cggccagagc gtttcgaagc
    14461 gccgcgatcc gatccgagga caacgatccg aacggttttc gcgccacgcc gcagccatag
    14521 ccggtgagct ccgccgccgc atacacatgc tggacgtagt ccccctccca aagcagagac
    14581 atgagcggtt cgagcttgcg ccagatcgat tggacatcgt cccatctttt ttctctggca
    14641 gccctggtga tcgcaacgca ggtccggggt gctatgttgg cgccacccca aatcaatccg
    14701 gagcaacctg caagcaggga gaaaggaacg agcggatcgg ctccgtttat aacaccgaga
    14761 ccggtgccga tcagtctgac ttgggagatc aggtcgccac tgctgtcctt aacggcctgg
    14821 aactcgggca agtcgcaaag catccgcaac aggctgggcg taatccgcac gccaacggcc
    14881 tgcggcacgt tatatccgat aatcggcagt cctacctttg cgacatcagc gtaaaatgca
    14941 aagatgctgc cgtcatccga gggaccttcg aaaaatggcg gcagaatcat cacaccatcg
    15001 gcgccgacat ccttggcgga ctgcgttctt gccacgacgt cagccgtgag caatgccgaa
    15061 gtctgagcga tcactttggc tcgcccggcc accacacgcg caccgcgctc gatgacggat
    15121 ttcgattcct caggcgtcag atagacatgc atgcctgtgc cggagctgaa cacgaagcca
    15181 tcgacgccgg cggccagata gcgctcgata agatcctcca accgtccaaa atcgacatga
    15241 ccgctcgcat tgaatggggt cgtgatcgcc agattgagac caggaaaagc aaacgtcgac
    15301 atttcaaatc ccttcgtcgg agctcagatc gacccagagg gtcttggtct ggcaatactg
    15361 atcgtgcgcg tggatggact tgtcccggcc accaaagccc gattgcttgt agccgccgaa
    15421 tggcgtggcg gcgtctccct ctccgtagca gttgaccgtg accgtacctg ccctgatcgc
    15481 gcgcgcggct cgaagcgccc tcgtgccatc gccggtgaaa actgaggccg caaggccata
    15541 ttcggtctga tttgcgagcg agactgcttc tgcaagcgaa tccaccggaa ttacagagag
    15601 gatcggcccg aaaatctctt cgcgaatatg tttaccccag tggcgggaga attcgataat
    15661 cgtcggttcg atcgtggttc caccgacgat gtttccacca gcgacgatct ttccgtcatt
    15721 ctccagatag ccactgacct tctcgaaatg aacggtcgaa accaatggac cgatgcgggt
    15781 ctcgggggcc agtggatcgc cgacgatcca ggtctttgcg acctccgcca gcttttgaag
    15841 cagccggtcc ttcacggccc gatgaacaaa caggcgcgaa attgccgagc agttctggcc
    15901 aacgttccag aacgcgccgt tgcagacatg agacgccacg gcatcaagat tgcgaacgtc
    15961 gtccatgacg atacaggggt tcttcccacc catttcgagg accacttcct tcaggttgct
    16021 ctctgatgag tatttgagga agcgccggcc cgtttcggtc gagccggtga aagagactgc
    16081 gtcgatatcc gcgtgacgac cgaccggttc tccgacgtcg ggaccatcgc cggtaacgac
    16141 gttgaagaca ccagccggga ttccggcgtc cagtgcaagc tcagcgaccc gcaatgtcgt
    16201 caacgatgtc tcttccgcag gcttgaccac gacggtgcag cccgccgcaa gcgctgggcc
    16261 gattttccag gcaagcatga gaagcgggaa gttccacggc aaaacgatgc ccacgacgcc
    16321 gatcggctcg cgcacaatga gaccaagaga agaatcgttt gccggtgaga cctggtcgta
    16381 gatcttgtcg acggcttcgg catgccacct gagactgttg atcgcctccg gcacgtcgat
    16441 gttgatgcag tcggtaatag tcttgccgct gtcaatgctt tccatcagcg ccaattcgag
    16501 tgcattttcc tcgatgacgg aggccaacct cagaagtgcc tgcttgcggt tggccggtgt
    16561 cagctttgac cagcgaccat cctcgaaggc ctgccgcgca gcggcaaccg ctctgtcaac
    16621 gtcggccgcg gagcccgagg ggaggtcagc cagaaccttg cctgtcgccg gattgatggt
    16681 gggatatgtc ttgccggaaa tggcggcgac cggctttcca ttgacgatca tgctggtggg
    16741 caacctgatg gattgcgcct gtgtcttgta gtcgaccgtg ccgtgcattc ccattctcct
    16801 ctggtgtctt cagtgaagac aaaatttgcc cagcgtttgc tctccacgcc aggtgagggc
    16861 accgctttga agtccgacag tcagtccacc ttgcgggtcg gtcgtcccgc gatcggatag
    16921 ccacctccca agctgcatcg gggctgcgga tgcaggctca gtcctcctcg aacgccgatg
    16981 ccgagccgat cggcaaagct ggttcaagcg agagcaacga ggcctatcct ataggcagca
    17041 ggcaccctta cttcaaatga gtattaatcg gggtcgggat aaccaggagt catggaggtt
    17101 cgaacgcctt tgagcttgcg tccacgtcga tagcgaatgt cgtggtccag gcatcaccta
    17161 ttcgctgccg gaagcatcgt gccacgggag gcggatcgac gagcggggcg accggcatga
    17221 ctgccggtct tctattctat tcgtcatttg ctgccggaga gctcaatgaa taccggacgt
    17281 gcaaatcgcg acccggggct cgcgatcagc ccggcgggcc cgccagttgc cctatagcct
    17341 ataggagcag gggaggcggg ccgtgtggcg ccattcgatg cactgttgcg actgaaaaag
    17401 ccagtcgccg ccggtatcct ccacgtccgg ccgacctgag cttcgattga ccggcgccgg
    17461 tttattcagg tcgagtacct tgacaccatg acctgtcgtt atggagggcg cgcacaaaaa
    17521 ctgatttgcg caagattgca ttatcgctga gcatgagaac gcaagcactt caagtgccag
    17581 tctgtcccta agcttatacc catggggtgc cgttgcttgg gaggcgggcc aggactccga
    17641 caaagcaacc agtcggagga aaaaagaaag agtgccccgt gcatggcgcc cgaggatcta
    17701 gggagaacga aatgaaattc aagattgccg ttgcagcgct cgcgataatg gccagtggta
    17761 ccttgtcggc cgccgccgaa caggtgaagt atggttggat cggtggcgtc aacgaggaac
    17821 tcttccaggg atatgtcgtc agggatggtc tgcgacagct gggattcgac gtcgaagagc
    17881 agcccgagca ggtccaggtc acacttgcct atacagcggt agcctccggc gacctgcagt
    17941 tttatccggc gttttggacg ccgctgcacg atgccttctg gaagggcgcc ggcggcgccg
    18001 acaagctgat gccggtcggc acgctcgtgc atggcgacat ccagggatat ctgatcgaca
    18061 agaagacggc cgatgcgacc ggcatcaaat atctcgagga cctgaaggat ccggaaaaag
    18121 ccaagctgtt cgatatcgac ggcaacggca aggcggatct gtttggctgc gacccgggtt
    18181 ggggctgcga acgcatgatc gagcatcacc tggatgcata cggcttgcgc gacaccgtcg
    18241 agcacaagca gggcggctat ttcgcgatca tggccgatgc gatccagcgc atcaaggacg
    18301 gcaagccaac gctctattat acttggatcc ccggctggat atccggcgtc ctgctcccag
    18361 gaaaggacgt cacatggctg aacgtcaaga cgacctcgct tcctgatggt cagaccggca
    18421 gcaccgaaat caagggcctc ggcaacctcg gcttcccggt caacgacacg aaggtgatgg
    18481 cgaacaccga atggctgaaa gccaatccct cggcaaagaa gtttctggag gagctctcca
    18541 tcccggtgaa ggatgtcacc gccgagaaca agctgatcta cgagggagag aaaaatcccg
    18601 atcagatcgc caagcatgcc acggactgga tagctgccca caaggcccag tgggacaagt
    18661 ggattgccga cgccaaggcc gcgaagtgac gcgataagag gagggtcgag cgctcgcccg
    18721 accctcctct ctactctcat ttgcgcattt gcgcgaggca cgcgagcgca aacgcagcgc
    18781 cacatgcctc agcgccgata gacttcccgc atcagttggc gggcgccgga tagcaagaaa
    18841 agcaggcccc agaccaggat gaggccgggc gcctgcaccc cgacaggaga ccgtttggaa
    18901 tgaactatct ggatagattt tccctggcgg gcaaacgcgc ctttgtcacc ggcggcgctc
    18961 gcgggatcgg tctggaaatc tgtcgggctc tcggacaagc gggtgcggcc attgttctgg
    19021 ccgatctggg tgaagaggca gcgcgttcgg ccgccgaagg cctggagcgc gaaggcctgg
    19081 aggtctgcta tcggatcgtc gacgtgacga agtccgccga tgtcaccgcg ctcgccgagg
    19141 agcttaatgc gcagcgcgcc gtcgatatcc tggtcaacaa tgctggcgtc ggccccaagg
    19201 ctgaggcgct cgacactctc gacgaggtgt ggtcgaaaac gatgaacgtc aactcggacg
    19261 cgcccttctg gtgttcgcgc gctttcggcc gtcacatgac tgcccgaggc tccgggaata
    19321 tcgtcaatat agggtcaatg tgcggcctga tcgtaaccca gcctcaaaac aatacctgct
    19381 atatcgcgag caagggcgcg cttcacatga tgacgaagtc gctggcttgc gcctgggcga
    19441 aattcaacgt gcgcgtaaac gcggtcgcac ctggctacat agccacggac atgctcgacc
    19501 gtttcaagac cgacaatgcg gtgtggtacg accgctggat tgaacaaacc ccactgggcc
    19561 gcatgggtac cccgcaggag atcgcctcgg ctgtgctcta tctcgcaagc gaggcttcga
    19621 gctactgcac aggtacgatc ctgtcggccg acggtggcta cacgtcattg tagccgaggg
    19681 aaagggcgac aatgtcccag cgggttgttt cgttgatgcc tgcagcgggg ctcaaggcag
    19741 ccgatgcctc tcaggccgaa actccaaggc gatcgacgcc agatgaagcc ttcagacgct
    19801 ttggtgaaat ccatcaaaga ccgtcgtgtc tggcagacaa tgttccagcg ggcggatgtt
    19861 agagcgcacg cacaccaaca ggttctcgag cggaggtaat tgatgtcgaa cattctgccg
    19921 cctcagatgg cacgcaagat gtacgccgac atgtttcgga tccggcagtt cgacatcaag
    19981 gtggtcgagc ttgtcgacga tgatctcatt cccggcagct gtcacgaata ttgcgggcag
    20041 gaagccgtcg cggtcggggt ctgcagcgca ctgacgaaaa aggacattct gacgagcacc
    20101 catagaggcc atggccatac gctggccaag ggaggcgacg tcggcaagat gtttgccgag
    20161 ctcttcggca agtccgccgg ctacaacaag ggccgcggcg gttccatgca tattgccgac
    20221 ttttccctgg gcattttcgg tgccaacgga atcgtgggag cgggggctcc aatcgcctgc
    20281 ggcgcggccc acaagttcaa ggcagccggc gagccgcatg tcgcggtgtc ttttttcggc
    20341 gacggggcca tcaatcaagg cgtcgttcac gaagcgatga accttgcggc gatctggcgc
    20401 ctgccggtca tcttcgtctg cgagaacaac ctttacgcaa tgaccacccc gcttcgcgac
    20461 gtgacaattc tggaacttta tgagcgcgcg caatcctatg ggatggcgag caccaaggtc
    20521 gatggcatgg atgtcggcgc cgtattcgat accaccttct ccgctgtcga gcgcgcacgc
    20581 gccggtgggg gcccgagctt cattcaatgc gacacctaca ggtattttgg cca
//
