LOCUS       NZ_AP024109            22617 bp    DNA     circular CON 10-NOV-2023
DEFINITION  Mesorhizobium sp. J8 chromosome, complete genome.
ACCESSION   NZ_AP024109
VERSION     NZ_AP024109.1
KEYWORDS    RefSeq.
SOURCE      Mesorhizobium sp. J8
  ORGANISM  Mesorhizobium sp. J8
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1
  AUTHORS   Kusaba,I., Nakao,T., Maita,H., Sato,S., Chijiiwa,R., Yamada,E.,
            Arima,S., Kojoma,M., Ishimaru,K., Akashi,R. and Suzuki,A.
  TITLE     Mesorhizobium sp. J8 can establish symbiosis with Glycyrrhiza
            uralensis, increasing glycyrrhizin production
  JOURNAL   Plant Biotechnol. 38, 57-66 (2021)
  REMARK    DOI:10.5511/plantbiotechnology.20.1124a
REFERENCE   2  (bases 1 to 6690860)
  AUTHORS   Maita,H. and Sato,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2020) Contact:Shusei Sato Tohoku University,
            Department of Environmental Life Sciences, Graduate School of Life
            Sciences; 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/10/2023 07:29:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,439
            CDSs (total)                      :: 6,376
            Genes (coding)                    :: 6,302
            CDSs (with protein)               :: 6,302
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 48 of 74
            Pseudo Genes (incomplete)         :: 41 of 74
            Pseudo Genes (internal stop)      :: 12 of 74
            Pseudo Genes (multiple problems)  :: 21 of 74
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:17:56
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 605242
            Orig. end                         :: 627859
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP024109.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22617
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="3"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22617
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22617
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12617](-)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12617)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    complement(1576..1591)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.79"
                     /tool="antismash"
     misc_feature    complement(1603..1616)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.22"
                     /tool="antismash"
     gene            complement(1719..3002)
                     /locus_tag="MJ8_RS02915"
                     /old_locus_tag="MJ8_05780"
     CDS             complement(1719..3002)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Wzy_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205267.1"
                     /locus_tag="MJ8_RS02915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05780"
                     /product="O-antigen ligase"
                     /protein_id="WP_201413005.1"
                     /sec_met_domain="Wzy_C (E-value: 6.1e-16, bitscore: 57.4,
                     seeds: 81, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIPKSILIDPDRNETYGTLAVAVSMIAFAYSPNFGQILILAYYA
                     VWLPLLFVDYRRFTWRLSSAWLPILLATYICFSVFWSQAAGISARAGVQYASHIVCAYV
                     AARTISVRTLVVGSLIGIFFVLLYSLGVGGYALDTLDGTVNFVGAFGSKNQIGFFSSLG
                     IFFCLAFMAFYRRNWLGFIWTAPIMLLSVYMLAIAHSATSVASLPAVIGVVMLLTMTKV
                     LSLRYRRVLFIVGAGALVTSVAAALNLGLLDVVLGIFGKDSTLTGRTYLWEQGWEAAQQ
                     HPLLGYGYAAYWVQGFADPERLWAEFYISTRSGFHFHNTYVETLVEIGFIGVTLLALVI
                     LRAFYGHVSKVVFGDWSADSVVLAGAIGLMLIRSFFEVEMLGPYFMASFIVYYGLFTLI
                     PLPAFRRRRVAIPAEDERPANGRVPAPV"
     gene            complement(3021..4112)
                     /locus_tag="MJ8_RS02920"
                     /old_locus_tag="MJ8_05790"
     CDS             complement(3021..4112)
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1088: acyltransferase 3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013884.3"
                     /locus_tag="MJ8_RS02920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05790"
                     /product="acyltransferase"
                     /protein_id="WP_201413006.1"
                     /transl_table=11
                     /translation="MQPAVPRHVYLNLDAIRGVAAISVMLYHFSPFLADGKVLPSSYLA
                     VDLFFLLSGFVIAHAYDRKIENGMGFGTFVAIRLIRLYPLYLAGTLLGFFYLLVKNRLM
                     PAEYMPLSEIGVQLTTGMFFIPLVSDAYHTIFPLNPASWSLFFELIVNIAYVAVFVLLT
                     RRVLTGIVAVSLVLLVVASVFAGTLDFGMTGNTILSGLPRVTFSFFLGVLLCRSMAHWQ
                     GSLGFLRRGLWVEGAILLTLAVFAIAPGGGARVAYDLAAIVIVFPIMVMTGAAAPTASL
                     LSGFYGWLGRISYPIYIIHTPMLMIIAGAGKAFSIDPFAHHPWFGLVMAASVIVIADIA
                     TRVYDEPVRRFLQRQMQRARAVA"
     gene            complement(4232..5410)
                     /locus_tag="MJ8_RS02925"
                     /old_locus_tag="MJ8_05800"
     CDS             complement(4232..5410)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Poly_export"
                     /gene_functions="transport (smcogs) SMCOG1186:
                     polysaccharide export protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872486.1"
                     /locus_tag="MJ8_RS02925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05800"
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="WP_225248111.1"
                     /sec_met_domain="Poly_export (E-value: 2.6e-16, bitscore:
                     57.9, seeds: 183, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQLAAAGDYQLGPLDKLNIRVAEWQTVDGTFRDWSAINGEYSVG
                     PGGTLSVPFVGDMQAAGKTTAEIASAIGLALQRKLALPDKPEASVEMAQFRPFYISGEV
                     QNPGQFPFVPDLTVLKAISIAGGIRRNADYGPQLGKDLVTAKGSYDIYDDQRIRLLVKR
                     ARIDADLAGKTSFEVPKEIEGDPRLPPIVADEMAILVSDQKSLKLKLDALDDLKGVLQS
                     EIESLQKKITNQQKQVDLAEKQAESIGPLAQKGLVANARLLSSQQSVTDLQGKILDYET
                     AILTAKQSISKAEQDAIDARNSLNSTLTANRQQTEADLNEATLKAGMQKGLIAQASDPA
                     TAASMTGDQEPTLLYSLVRVADGKTSEVEAKEDTPVLPGDVIKVKLAPTASQ"
     misc_feature    complement(5504..5517)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 20.59"
                     /tool="antismash"
     gene            complement(5596..6273)
                     /locus_tag="MJ8_RS02930"
                     /old_locus_tag="MJ8_05810"
     CDS             complement(5596..6273)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bac_transf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1146: sugar transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205264.1"
                     /locus_tag="MJ8_RS02930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05810"
                     /product="sugar transferase"
                     /protein_id="WP_201413008.1"
                     /sec_met_domain="Bac_transf (E-value: 6.3e-68, bitscore:
                     226.3, seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRDVAKSANAGFAQAGVDFPPPIGGLIKRSFDIVGSLAGLILLSP
                     LFLMVALLVKLSDNGPIFYGHKRIGRGGRIFSCLKFRTMVPDGERVLAAYLAANPEANA
                     EWIATRKLKNDPRVTRVGAVLRKLSLDELPQILNILQGDMSLVGPRPVVRDELEIYGSA
                     AVYYLKSRPGLTGLWQVSGRNDVSYDTRVAFDRHYVENWSLFEDIRIIFKTVPAVWMSR
                     GSY"
     misc_feature    complement(6311..6325)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.76"
                     /tool="antismash"
     misc_feature    complement(6376..6390)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 20.11"
                     /tool="antismash"
     misc_feature    6440..6456
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.99"
                     /tool="antismash"
     gene            6880..7152
                     /locus_tag="MJ8_RS02935"
                     /old_locus_tag="MJ8_05820"
     CDS             6880..7152
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872483.1"
                     /locus_tag="MJ8_RS02935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05820"
                     /product="exopolysaccharide production repressor exox"
                     /protein_id="WP_201413009.1"
                     /transl_table=11
                     /translation="MSLPKFIIGMMFALAIVIGWSYFDGASAGTILLRAIVCAVIIQAG
                     YFLLVFAMVGRATPTSADKVRESDRGAILNKVAEGEKLSARRSLH"
     gene            complement(7126..9015)
                     /locus_tag="MJ8_RS02940"
                     /old_locus_tag="MJ8_05830"
     CDS             complement(7126..9015)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205262.1"
                     /locus_tag="MJ8_RS02940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05830"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_201413010.1"
                     /transl_table=11
                     /translation="MGWSSKLRLSFFRDVAGFGAMMAHVGGRRTATALAFLILGSLTEG
                     ASILLLVPLLRLIGKAEQNYAVRVPEALHWLVPGGTLSLATVLSLLVALVTLQAVFNRF
                     KSVYMARLLYDFVNRVRMNLFESVGKARWGVFTRIRSSDLDHALNGDVDRVQVAAFSLL
                     MLTQATLLLTGYLVVSLFISPVMTCFAIFIGLVLLIALQPFRARASAYGRTLTGNRQDQ
                     YRTVSEFLGGIKVAKSLNVEASYFAELGATLDRMKSDNIAFVRNSSLGTALFQVTSVIG
                     LSLFIYIALVGFHLSLAEIVVLLLVFMRVAPRFMDMQLQTQQVLINLPAYASMQSLQAR
                     FDAEREPEESAAEQGARLSLETGLNIRDICFAYGEGDGHPVVSDITFGLPAGKVTALIG
                     PSGSGKSTIADMLLGLLEPTSGRILADGVEITARNRRAWRDQVAYVPQDVFLLHDTIAA
                     NLRLAAPGANDDELWAALRNAHAADFVERLALRLETVVGDRGVRLSGGERQRIALARAL
                     LRKPSLLILDEATSALDWQNQSLIAQSIESLRGQMTILTIAHRPSMIAFADWVVAIENG
                     RIVEVGQYRRLKAKAGSRLARMLSGEQAERDTAVEAGPAPAPVERSVEAAPGA"
     gene            complement(9024..10004)
                     /locus_tag="MJ8_RS02945"
                     /old_locus_tag="MJ8_05840"
     CDS             complement(9024..10004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913074.1"
                     /locus_tag="MJ8_RS02945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05840"
                     /product="serine kinase"
                     /protein_id="WP_225248112.1"
                     /transl_table=11
                     /translation="MNLPIASLARPGPATETAAPGGRVRHFYKAYGLIVRSEVALPELE
                     PAAPATPDLEIAVGPVDFPDAASQGATAFRFESTRQYLSWQAVGTFMISDGKRIDVDPA
                     PGVGDPLLAFPLLGPVMALALHRRGLLVLHASAIAVGGKSVIFMGDKGAGKSTTAGAMI
                     RAGHRLLTDDVVALDLSDPGRPMILPGFPQLKLAADAAGAIRLEQAEVRPQVHPQIDKA
                     QHRLRDGFAGEAVPVSRLYVLERGARAAISPLTGAAALPAVIKFSYITRFGRQALPGDS
                     ASVHLGQCAQVAGRVGLSRLEVPAGLARIDEAVAAIETDLASETR"
     gene            complement(10001..11914)
                     /locus_tag="MJ8_RS02950"
                     /old_locus_tag="MJ8_05850"
     CDS             complement(10001..11914)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205260.1"
                     /locus_tag="MJ8_RS02950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05850"
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_201413011.1"
                     /sec_met_domain="GATase_7 (E-value: 1.6e-25, bitscore:
                     87.9, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 2e-60, bitscore:
                     203.9, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGVAGIVHDDNGRPAGAGDIQEMLSRMRHRARDGSSWWSEGSVA
                     LGHAWLDTTGEDGHGPLTMAGGKLAITADCRLDNRDELLARLGLRDRSMADAALIMRAY
                     LKWGEACPIYLQGDFAFAIWDGERQALFCARDHFGVKPFYYHATGKRFAFASEIGPILA
                     LDGVGRRLSEHQISGFLAGLPDDPQATPYAEIFRLPARHSMMVADRQVALRRYWHIEPS
                     QRPPRSDAAEEFRHLFAQSVENRMRGTPAVGAMLSGGLDSSSIACVAGVRAAAKRNPKL
                     KTFSLVFDKGSPMDEKPFIDAVLADNPLDATLIAVGNYAPFAEFDRILEEQEGTFLAPG
                     LSLTRDLYRTAGAKGVKVLLDGHGGDEVVSQGHGHLHELANAGRWLELWRELRSAANTY
                     GEGMLSLYFKFLTIYGPAWRIARVRSMARRVVGKARRRPAQTARAAWTGLVNPELARRT
                     DLAERFHRSGYMPAAVTASEALTHRWLLSTGLVPHAFEVLDKAAANFGVEPRYPFWDKP
                     LVEFCLALPGEEKLRDGFGRHVLRRAMQGILPPAVQWRRDKIDFTANLVNGMLGNHREL
                     LDRLLISDSERIAPYVNLPEVNAVYERLLSRPEQATLPDVQHVWRSVSLSLWLRQVQGS
                     PA"
     gene            complement(11918..12334)
                     /locus_tag="MJ8_RS02955"
                     /old_locus_tag="MJ8_05860"
     CDS             complement(11918..12334)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314227.1"
                     /locus_tag="MJ8_RS02955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05860"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_225248113.1"
                     /sec_met_domain="PF13471 (E-value: 2.7e-20, bitscore: 71.1,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRNWSRLRSLSGREMLFLARCLVVVSAVRLALTLSSYNRVRGAVT
                     RLDARRDASVADLRRVAWGVAAAARLVPGATCLTQALAGQYLLARQGNASKISIGIEKG
                     TGSTLKAHAWLMSGNHIVLGGSINGFAHLVDHGS"
     gene            complement(12318..12617)
                     /locus_tag="MJ8_RS02960"
                     /old_locus_tag="MJ8_05870"
     CDS             complement(12318..12617)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862839.1"
                     /locus_tag="MJ8_RS02960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05870"
                     /product="PqqD family protein"
                     /protein_id="WP_201413012.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1e-25,
                     bitscore: 88.1, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 2.1e-18, bitscore: 64.9,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDWNPSDHDRVVATGEAVACEFGPGLALLHLKSNVYYSLNGVGAF
                     IWEQIQEPRSILDIRSAVLARYNVDAERCKADVEGLLRGLSEAGLARLHREELV"
     aSDomain        complement(12342..12581)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_MJ8_RS02960_Stand_Alone_Lasso_RRE.1"
                     /evalue="4.50E-28"
                     /identifier="RREFam002.1"
                     /label="MJ8_RS02960"
                     /locus_tag="MJ8_RS02960"
                     /protein_end="92"
                     /protein_start="12"
                     /score="88.1"
                     /tool="antismash"
                     /translation="ATGEAVACEFGPGLALLHLKSNVYYSLNGVGAFIWEQIQEPRSIL
                     DIRSAVLARYNVDAERCKADVEGLLRGLSEAGLAR"
     gene            complement(12779..12931)
                     /locus_tag="MJ8_RS02965"
                     /old_locus_tag="MJ8_05880"
     CDS             complement(12779..12931)
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212599.1"
                     /locus_tag="MJ8_RS02965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05880"
                     /product="lasso RiPP family leader peptide-containing
                     protein"
                     /protein_id="WP_095769314.1"
                     /transl_table=11
                     /translation="MEQNVEKHEYETPSLTVHGSIETITQGGAGTTALDASFPAHTPVG
                     ELTFS"
     CDS_motif       complement(12854..12931)
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MEQNVEKHEYETPSLTVHGSIETITQ"
                     /locus_tag="MJ8_RS02965_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       complement(12779..12853)
                     /prepeptide="core"
                     /locus_tag="MJ8_RS02965_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="-0.30"
                     /molecular_weight="2286.4"
                     /monoisotopic_mass="2285.1"
                     /core_sequence="GGAGTTALDASFPAHTPVGELTFS"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MEQNVEKHEYETPSLTVHGSIETITQ"
                     /leader_location="[12853:12931](-)"
     misc_feature    13056..13074
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.85"
                     /tool="antismash"
     misc_feature    complement(13215..13229)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.51"
                     /tool="antismash"
     misc_feature    complement(13218..13231)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.15"
                     /tool="antismash"
     misc_feature    complement(13358..13378)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 22.04"
                     /tool="antismash"
     gene            13433..14665
                     /locus_tag="MJ8_RS02970"
                     /old_locus_tag="MJ8_05890"
     CDS             13433..14665
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314224.1"
                     /locus_tag="MJ8_RS02970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05890"
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_201413013.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 2.9e-37, bitscore:
                     127.0, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVQDGSYERLRAAGCANEVAYVQACLRLFFTPRAKITDGPADLP
                     ALTVSAAPISAAPVSAANVADIARLNKVAVFLLKALKHAPADAVPAELAGWLDGYRRRT
                     MSMNAACIGDSIAIDRALRESGVDFVFLKGPLQQHLLYGDHFMKPSGDVDILVSPARFG
                     QAREALRAIGYEVAGKSRSVWWIRFLGEQHMVREDGARASTVDLHHRLQQPGSPSPRDI
                     DGFLRRKREVEVAGAAMPVISAADTLLLSSISVAKAFFNREPCAGYVCDIRASAGRLNE
                     AEQQRVLDHAAGQGLADTLLLGLRAADVLLGATGTLLSERATRILARIDNADLFHMVMA
                     PWLASLRWPQRRHVLWELCGREPVRYLAEAGWAASADLSRRIFERPATIGPAQGEMIKI
                     MGGAANHASER"
     gene            14670..15698
                     /locus_tag="MJ8_RS02975"
                     /old_locus_tag="MJ8_05900"
     CDS             14670..15698
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325268.1"
                     /locus_tag="MJ8_RS02975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05900"
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_201413014.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 3.9e-24,
                     bitscore: 84.0, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVTTPITNPGVCVIIAARNAARTIPGAIASALREAEVAEVIVVD
                     DASTDDTREVALAADDGSGRLAVIRFDVNRGPSAARNAAIQASRSPFISILDADDFFLE
                     GRFRRLFASADWDFAADNIMLIRDDAATDLAKITAPPFEAEPEFLDFERFIDGNISRRR
                     VRRGELGFLKPVISRTFLDRHGLSYDENLRLGEDYELYARAVACGARFKVIKSCGYGAI
                     VRADSLSGRHRTQDLKRLADADLALLQIDSLPERSKAALRRHERHVRDKYRLRNFLDVK
                     AERGLASAAAYAFASQSNLIPIVRGVATDKLDALFRRTGIVARQQIPPMRFLMAATSAA
                     NE"
     misc_feature    complement(15728..15737)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.17"
                     /tool="antismash"
     gene            15823..16200
                     /locus_tag="MJ8_RS02980"
                     /old_locus_tag="MJ8_05910"
     CDS             15823..16200
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205254.1"
                     /locus_tag="MJ8_RS02980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05910"
                     /product="hypothetical protein"
                     /protein_id="WP_201415284.1"
                     /transl_table=11
                     /translation="MASIFGFRSRDPARDRQTDLQRFDRLAKLFDQIAAEIEAEKTGLE
                     NRYKSTAANAAFLVEAMENGSASASKGSDVSAMTSSILNCERRIAELARQKGLMKELRH
                     SLDAIVGDGFDRPAAQATVRR"
     gene            16329..17213
                     /locus_tag="MJ8_RS02985"
                     /old_locus_tag="MJ8_05920"
     CDS             16329..17213
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205253.1"
                     /locus_tag="MJ8_RS02985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05920"
                     /product="family 16 glycosylhydrolase"
                     /protein_id="WP_201413015.1"
                     /transl_table=11
                     /translation="MNADPDNTSAVAACATSHAPWSGLTSLRLGFVGALLAIAATLAGS
                     LHPAHAQEAQQELKSAPSFVDDFSNFNQSRWFVSDGWTNGAHQNCTWSKDLVRLSDGVL
                     SLGFEKRKLKDRDFACAEIQTKQRYGYGVYEARMKTGTGSGLNAAFFSYIGPQDKQPWD
                     EIDFEVLTKDPSKVQVNSYIQGKPKNGKLVDVEGGADKGFNDYGFVWEKDRLRWYINGK
                     LVHEVTNPDELPTHSQKIFFSLWGSDKLTNWMGAFTDPGSRVIMQVKRVAFTALGQPCQ
                     FPESLACSVSNSN"
     gene            17385..18716
                     /locus_tag="MJ8_RS02990"
                     /old_locus_tag="MJ8_05930"
     CDS             17385..18716
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1152: nucleotide sugar dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872478.1"
                     /locus_tag="MJ8_RS02990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05930"
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="WP_201413016.1"
                     /transl_table=11
                     /translation="MNLTVFGIGYVGLVQAAVLAEVGHEVVCVDVDEKKVERLNQGLIP
                     IFEPGLESLVKENHAAGRIRFTTDAAAAVRHGQIQMIAVGTPPGEDGSADLRYVLAVAE
                     TIGREMDGPKIVVGKSTVPVGTCEKVKAKIAETLKKRGREDLAFDVASNPEFLKEGSAV
                     ADCMKPDRIIVGTSSEDTEMVMRELYAPFNRNHEKMIVMDVRSAEFTKYAANCMLATKI
                     SFMNEMANLAEQLGADIEEVRKGIGSDPRIGYHFIYPGLGYGGSCFPKDVRALIKTAEG
                     VKFDAKLLRAVEERNNAQKSVLFDKVNHYFKGALRGKTFAVWGLAFKPNTDDMREAPSR
                     TLMEALWAAGAKVQAYDPEAMQECQAIYGLREDLLLCGTKEAALRGANALLICTEWKSF
                     RAPSFDALKDALTTPVIFDGRNLYDPKVIARYGIEYFSIGRMAA"
     gene            18807..20255
                     /locus_tag="MJ8_RS02995"
                     /old_locus_tag="MJ8_05940"
     CDS             18807..20255
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872476.1"
                     /locus_tag="MJ8_RS02995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05940"
                     /product="GMC family oxidoreductase"
                     /protein_id="WP_201413017.1"
                     /transl_table=11
                     /translation="MVLDVRPEQIASEHFDLVVIGSGFGSSFFLHEFLKRRKARVLVLE
                     WGRHNTHEWQLSQNANTDIDDESTYDTDNPDKPWNYTIGLGGGTNCWFAQTPRFHPNDF
                     RLKSTYGVGNDWPISYDELEPFYGDAEEIMSISGDPDMARMLPRSRPFPQPPHRMSTPD
                     RMMKAAQPDQHFVMPTARARVATPQRASCCANLRCWLCPADAKFTVNNGLMHVFQHPDV
                     SVCLGAEVRRFDHAGGAVRSVAFVRDGKEYSVGGDLFILGANAIQSPAIMLRSGLGGEF
                     VGLGLHESYGWNYEAYLGGVDNFDGSTITTGLNFGLYDGPHRAEHAAALVYFENRWQHG
                     MRAEKGRLRQVLPLVIVTENLLDPENRVILDKNENAFVSFKGASDYATKGMAKALERLP
                     ELLKPLPVEQIFDRGIRPTESHVQGTLRMGTGPADSVVDRDMIHHQLRNLVVVGTSTYP
                     SCSCANPSLTAAALSLRAAGKLAA"
     gene            20267..20710
                     /locus_tag="MJ8_RS03000"
                     /old_locus_tag="MJ8_05950"
     CDS             20267..20710
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872477.1"
                     /locus_tag="MJ8_RS03000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05950"
                     /product="hypothetical protein"
                     /protein_id="WP_201413018.1"
                     /transl_table=11
                     /translation="MKITRRTALAIVAGGIASTGIAAAAVSSFSDEDLVRVTLEKYFGP
                     LNMRIEHLQQFVLEFQNRNPWIFPSAKVADVVTLAETVKLGKARELLPQDKRRDLRHFD
                     RWVIAEFHMLTDYAWRSSPDDPVRFTGWHPCTNPFADLELQNA"
     misc_feature    complement(20312..20328)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.87"
                     /tool="antismash"
     gene            20804..22024
                     /locus_tag="MJ8_RS03005"
                     /old_locus_tag="MJ8_05960"
     CDS             20804..22024
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1123: polyprenol-monophosphomannose synthase ppm1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF021616.3"
                     /locus_tag="MJ8_RS03005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MJ8_05960"
                     /product="glycosyltransferase family A protein"
                     /protein_id="WP_201413019.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 5.7e-38,
                     bitscore: 129.0, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVQRKPTVTVIVPTFNREKFLPEAIDSLLRQTVAPDQILIVDDG
                     STDNTAAVAAAFPAPVEYYRKENGGKSTALNFALKHCTGDFVWIFDDDDVAHPTALERF
                     LAAFEREPDADFAFGEYARFQRSAQIEEEDYEIVAFGHVTQDNFFPSHLEYCHVHQPGL
                     LVRRECYDEVGGFNETLVRSQDYEMMLRLARRFKAIRVEGLMFFQRQHDMVRGSTKVVI
                     SYTDRIKAWGSYDRVIMEEIYKSVDLHEYLDRSEQALPKTADLEMQALLQRSSIMARKG
                     LWHHAAADIRRYAQLAERAGKTSLSDKERAIVRRTFEAYDTGLASATADAFVSAVKGWK
                     AGPLKADIVREYMHSFPYFLARDLKAGKLVPASKLLREYSTLSLSGSVSRLCRAMTSKL
                     PLQRGAS"
     misc_feature    complement(22163..22178)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.44"
                     /tool="antismash"
ORIGIN
        1 tgctcgccgg ctactggccg cagagcatga acacgctagc gctggtgctg ctggcggtgc
       61 cgatctccac cgtgctcggc ttcctgcttg gcgttctggg ctacgctcgc ccgcgctggc
      121 gtccggtgct caatggcgcg cttgacctga tgcagaccgt gccggctttc gcctatctca
      181 ttccgctcct gctcctgttc ggcttcggtc cggtggtcgg gttgatcgcc tcggcgatct
      241 acgcgacgcc gccgatggtg cgcaacacca tgctcgggtt ggagcgcgtg ccggcggcga
      301 tcagcgaggc cggcctgatg agcggctgca cgcgccgcca gcaattctgg caggtcgagg
      361 tgccgacggc gctgccgcag cttctcgtcg gcttcaacca gacgacgatg gcggcgctgt
      421 cgatggtgat cgtcgccgcc atcatcggcg gcttcgaaga catcggctgg gaggtgctct
      481 cctcgatgcg caaggccgaa ttcggccaga gcatcctctc cggcctggtt atcgcgctgc
      541 tcgccatcct catcgaccgc ctcaccatcg gcttcgccaa ggcgccggag accgggccga
      601 cgcccgccat gcgctggatg acgccgcgcc ggctggccct gtcgctgtcc gtcgcgctgg
      661 cgctcgccat cgtcatccgg ctggccgcgc ccgatgcgac gcttctgccg gaaaccgggc
      721 tgcgcatgca gatgggcgcc gtcaaccaat ggctgctggg gctcgtgcgc gactatgcct
      781 ggttcttcaa cggcgtgcgc aatgcggtgc tctactgcct gcttctgccg ctgcgcgtcg
      841 gtatttccgg ctcggcgacg ccggcgatgt ggggcttctc gctgccgccg tccgtgctgg
      901 tcgcttatgt cgccctggtg ctggcctttg ccatcttggc gttgcgcggc ttcggttggc
      961 gcggggcact ggcggtgctg gccgccggct ttgtcttcta taccggcttt ctcgatctgc
     1021 cctggcctgt cttcatcctt gccgtcgcct tgctcgccgg ccaggtgggc gggccgcggc
     1081 tcggtttttt cgcgcttgtc ggcttcgggc tgatactggt caatggccta tggcctcagc
     1141 tggtgcagtc gctctatctc tgcacgctgg cggtcctgct ttgccttctg gtcggcggcg
     1201 cgctcggaac atgggcggcg cataacgatc gcgtctcgca tatcctgaag ccgatctgcg
     1261 acgcgctgca gacgatgccg caattcgtct tcctcatccc ggcgctcatg ttcttcaagg
     1321 ttggcgagtt cacggcgctg atcgccatca tgctctacgc catcgtgccg ccgatccgct
     1381 atgtcgagca cgggctgcgg catgtccgcc ccgatgtcgt cgaggcggtc gagcagatgg
     1441 gcgcgacgcc gctgcagacc ttgctgcagg ccaagctgcc gctcgctctg cccgtcgtca
     1501 tgctcggcct caaccagacc atcatggcgg ccctgtcgat gctggccatc gccgcccttg
     1561 tcggcacgcg tgatcttggc caggcggtct atgtcgcgct gggcaaggcc gatgccggca
     1621 tgggcctgat cgccggcctg tcgatcgcct tcctggcgat ccttgccgac cggttgatcc
     1681 aggcagccgt tgcgcgttcg gccgctgctt cacattgatc agacaggcgc cggcacgcgc
     1741 ccattggcag gcctctcatc ctcggctgga atggccaccc tgcggcgccg gaacgccggc
     1801 aaggggatca gcgtgaacag gccgtaatag acgatgaagg acgccatgaa gtaggggccg
     1861 agcatctcga cctcgaagaa ggagcggatc agcatcaacc cgatcgcgcc ggcaagcacc
     1921 accgaatcgg cgctccaatc gccgaacacc actttcgaca catggccgta gaaagcgcgc
     1981 aggatcacca gcgccagcag ggtgacgccg atgaagccga tctcgaccag cgtctcgaca
     2041 taggtgttgt ggaagtggaa gccggagcgg gtcgagatgt agaattccgc ccacagccgt
     2101 tccgggtcgg caaagccctg cacccaatag gcggcatagc catagccaag cagcggatgc
     2161 tgctgcgccg cctcccagcc ctgctcccac agataggtgc ggccggtgag cgtcgaatcc
     2221 ttgccgaaga tgccgagcac gacatccagc aagccgaggt tgagcgccgc cgcgacgctt
     2281 gtcaccagcg cgccggcgcc gacgatgaac agcacgcggc gatagcgcag cgacaagacc
     2341 ttggtcatgg tgaggagcat cacgacgccg atcaccgcag gcagcgaggc gaccgacgtc
     2401 gccgaatggg cgatggcgag catatagacc gacagaagca tgatcggcgc tgtccagatg
     2461 aagcccagcc agttgcgccg gtagaaggcc atgaacgcaa ggcagaagaa gatgccgagc
     2521 gaagagaaga aaccgatctg gttcttggag ccgaaggcgc cgacgaaatt gaccgtgccg
     2581 tcgagcgtgt cgagcgcgta accgccgact ccgagtgaat agagcagaac gaaaaagatg
     2641 ccgatcagcg agcccaccac cagcgtgcgc acgctgatgg tgcgcgccgc gacataggcg
     2701 cagacgatgt gcgaggcata ttgcactccc gcgcgcgcgc tgatgcccgc cgcctgggac
     2761 cagaacaccg agaagcagat ataggtggct agcagaatgg gcagccaggc gctggaaagc
     2821 ctccaggtga agcgccggta gtcgacgaag agcagcggca gccacacggc ataataggca
     2881 aggatcagga tctggccgaa attgggcgaa taggcgaagg cgatcatgga gaccgcgacg
     2941 gcgagggtgc cgtaggtttc gttgcgatcg ggatcgatca ggatggattt gggaatcttc
     3001 ataccggatc ttgtctgttc ctaagccacg gcccgcgcgc gctgcatctg ccgctgcagg
     3061 aagcgccgca ccggctcgtc atagacgcgc gtggcgatgt cggcgatgac gatgacagag
     3121 gccgccatca cgaggccgaa ccagggatga tgcgcgaaag gatcgatcga gaaggccttg
     3181 ccggcgccgg cgatgatcat cagcatcggc gtgtggatga tgtagatcgg gtaggaaatc
     3241 cgcccgagcc agccatagaa gcccgacaga agcgacgcgg tcggcgccgc cgcgccggtc
     3301 atcaccatga tcgggaagac gatgacgatg gccgcgaggt cgtaggccac acgcgcgccg
     3361 ccgccgggcg cgatggcgaa gacggcaagg gtgagcagga tcgcgccttc gacccagagc
     3421 ccgcgccgca ggaagccgag gctgccttgc caatgcgcca tcgatcggca gagcagcacg
     3481 ccgaggaaga aggagaatgt cacgcgcggc aggccgctga ggatggtgtt gccggtcatg
     3541 ccgaaatcca gcgtgcccgc gaaaaccgag gcgacgacga gcagcaccag gctgacggcg
     3601 acgatccccg tcagcacgcg ccgtgtcagc agcacgaaga ccgcgacata ggcgatgttg
     3661 acgataagct cgaagaacaa cgaccaagat gccggattga gcgggaagat cgtatgatag
     3721 gcgtcgctga ccagcgggat gaagaacatg ccggtcgtca gttgcacgcc gatctcggag
     3781 agcggcatgt attcggccgg catcagcctg ttcttcacca gcaggtagaa gaagccgagc
     3841 agcgtgccgg cgagatagag cggatagagc cggatcaggc ggatggccac gaacgtgccg
     3901 aagcccatgc cgttctcgat cttgcggtca taggcatgcg cgatgacgaa gccgctgaga
     3961 aggaagaaaa gatcgaccgc gagatagctc gacggcagca ccttgccgtc ggccaggaat
     4021 ggcgagaaat ggtagagcat cacgctgatc gccgccacgc cgcggatggc gtcgaggttc
     4081 agatagacgt gacgcgggac tgcgggttgc atgttatgcc ttcaagcggt ccaaacagat
     4141 cgctcgcccg cgccgcgacg cgaacgagcc agggcttgtc cctccagcct ccgtttcccg
     4201 gcccgattcg ggtaagccgg ggacggcggc gctactggct ggccgtcggc gccagcttta
     4261 ccttgatcac gtcgcccggc aggacgggcg tgtcttcctt ggcctcgacc tcgctcgtct
     4321 taccgtcggc aacgcgcacc agcgagtaga gcagggtcgg ctcctggtcg ccggtcatgc
     4381 tggccgccgt cgcggggtcc gacgcctgcg cgatcagacc cttctgcatg ccggccttca
     4441 acgtcgcctc gttcaggtcg gcctcggtct gctggcggtt ggcggtaagg gtggagttga
     4501 gcgagttcct cgcgtcgatg gcgtcctgtt ctgccttgct gattgactgc ttggcggtga
     4561 ggatggccgt ctcatagtcg aggatcttgc cctgcaggtc ggtgaccgac tgttgcgagg
     4621 aaagcagccg cgcattggcg actaggcctt tctgcgccag cggaccgatg ctttcggcct
     4681 gtttctcggc caggtcgacc tgtttctgct ggttggtgat cttcttctgc aacgattcga
     4741 tttcggactg caggacgcct ttcagatcgt cgagggcatc gagcttcagc ttcagcgatt
     4801 tctggtcgga gaccaggatc gccatttcgt cggcgacgat gggcggcagt ctcggatcgc
     4861 cctcgatctc cttggggacc tcgaaactcg tcttcccggc gaggtcggcg tcgattctgg
     4921 ctcgcttgac gaggaggcgg atacgctggt cgtcatagat gtcatagctg cccttggcgg
     4981 tgaccagatc cttgccgagt tggggaccat agtcggcgtt gcgccttatg ccgccggcga
     5041 tgctgattgc cttgagcacg gtcagatcgg gcacgaaggg aaactggccg ggattctgaa
     5101 cctcgcccga aatgtagaac ggtcggaact gcgccatctc gaccgaggct tccggcttgt
     5161 cgggtagcgc tagcttacgc tgcagcgcga gcccgatcgc cgaagcgatc tccgccgtcg
     5221 tcttgccggc ggcctgcatg tcgccgacga acggcaccga cagcgtcccg cccgggccga
     5281 ccgaatactc gccattgatg gccgaccagt cgcggaacgt gccgtcgacc gtctgccatt
     5341 cggcgacgcg gatgttgagc ttgtccaggg ggccaagctg ataatcgcca gcggcagcca
     5401 attgcggcat ggccagcgac gccgcaagca agaagcccgc gacgcgggct ggtgtccggg
     5461 atatgagact gcaggcgaaa gacagtccga agaggtcttt tgtcaaatga acgtttccga
     5521 tccgctttgg ccccatccgc ccgatgagat cactttcgga tgcccgcccc gcgatcggcg
     5581 gagcaggccc gtcggtcaat agctgccgcg cgacatccac acggcaggaa ctgtcttgaa
     5641 gatgatgcga atatcctcga acagcgacca gttctcgaca tagtggcggt cgaaggcgac
     5701 gcgcgtgtcg taggacacgt cattgcggcc gctcacctgc cacagcccgg tgagacccgg
     5761 gcgcgacttc aggtaataga cagcggcgct gccatagatc tccaattcgt cgcgcaccac
     5821 cgggcgcggg ccgacaaggc tcatgtcgcc ttgcaggata ttgaggatct gcggcagctc
     5881 gtcgaggctg agcttgcgaa gcaccgcgcc gacgcgagtg acgcgcggat cgttcttcag
     5941 cttgcgcgtt gcgatccatt cggcgttggc ttccggattg gccgccagat aggcggcgag
     6001 cacgcgctcg ccgtccggaa ccatggtgcg gaatttgagg caggagaaaa tcctgccgcc
     6061 gcggccgatc cgcttatggc cataaaagat cggaccgttg tccgaaagct tgaccagcag
     6121 ggcgaccatc aggaagagcg ggctgagcaa aatcagccct gccagggaac cgacgatatc
     6181 gaagctgcgt ttgattaaac cgccgatggg gggtggaaaa tcaacgccgg cctgggcaaa
     6241 acccgcatta gccgacttgg cgacgtccct catgcagaag ctccatacgt tgaacggatg
     6301 gtttacggca agatatcaaa aaaatgctgc agcgcaacac acaatttccc atcggtctga
     6361 aatgttcacg tcatgaattg actaaataat aaacctggcc tgcccaaatt ttcacctttg
     6421 tggcacgttt atgacaaggt gacggaaaac caggaaaatg tagtccgggt tttgggccgt
     6481 ttttggacag tgcattcttt gctgcagaag aagttcggtt gcagggcgcg agaatcactc
     6541 gcggcggtaa gttattcaga taaaatacta atcaaaagca cgagttttac tgaggacgca
     6601 gcggcggcat tgaatagcta gcggagccac agtgtgcgtc gcagcatggc ggagtcgatc
     6661 tcgtaaaatc cttggaaacc atacgttcag ggccttgctg gaaccggccc gccttaatag
     6721 agtcgcaaat tgttcctgtc attgtctgca tgggtaagga aggccggaag caaccggccg
     6781 gattgggcag ccacaaagag ctctttggcg caacggggag tacgcgggtg ggtaagtcgc
     6841 agcttctgca tgctcgtctc ggttgaaggg ccggcgaaca tgtctctgcc aaaattcatc
     6901 atcggcatga tgttcgcgct cgcgatcgtc atcggctggt catatttcga cggcgcatcg
     6961 gcgggcacca tcctcttgcg cgccatcgtt tgcgccgtca ttatccaggc gggctatttc
     7021 cttctggtct tcgccatggt cggcagagcc acgccgacat cggcggacaa ggtccgtgaa
     7081 tccgaccgcg gcgcgatcct gaacaaggtc gccgaaggcg aaaagctaag cgccaggcgc
     7141 agcctccact gaccgttcga ccggcgccgg ggccggtccg gcctcgaccg cggtgtcgcg
     7201 ctcggcctgt tcgcccgaca gcatcctggc aagacggctg ccggcttttg ccttcagccg
     7261 ccgatattgt ccgacctcga cgatgcgccc gttctcgatg gccacgaccc agtcggcgaa
     7321 ggcgatcatc gatggccggt gcgcgatggt caaaatcgtc atctggccgc gcagcgactc
     7381 gatcgattgg gcgatcagcg attgattctg ccagtcgagc gcgctcgtcg cctcgtcgag
     7441 gatgaggagc gagggcttgc gcagcagcgc ccgcgccaaa gcgatgcgct gccgctcgcc
     7501 gccggagagc cgcacaccgc ggtcgcccac caccgtctcc agccgcaatg ccagccgctc
     7561 cacgaaatcg gccgcatggg cgttgcgcag cgccgcccaa agctcgtcgt cattggcccc
     7621 cggcgctgca aggcgcagat tggcggcgat cgtgtcatgc agcaggaaca cgtcctgcgg
     7681 tacgtaggcg acctgatcgc gccaggccct gcggttccgg gcggtgatct ccacgccgtc
     7741 ggccaggatc ctgccgctgg ttggttcgag caggccgagc agcatgtcgg cgatcgtgct
     7801 cttgcccgag ccggaagggc cgatcagcgc cgttaccttg ccggcaggca ggccgaaggt
     7861 gatgtcgctg accaccgggt gcccgtctcc ttcgccatag gcgaagcaga tatcgcggat
     7921 gttgaggccc gtctcgagcg agagtctcgc gccctgttcg gccgcgcttt cttccggctc
     7981 gcgctcggca tcgaaacggg cctggaggct ctgcattgaa gcataggccg gcaggttgat
     8041 cagcacctgc tgggtctgga gctgcatatc catgaagcgc ggcgcgacgc gcatgaaaac
     8101 caggagcagc acgacgatct cggcaagcga caggtggaag ccgaccagcg cgatatagat
     8161 gaacaggctg agcccgatca cgctcgtaac ctggaacagc gcggtgccca gcgacgagtt
     8221 gcggacgaag gcgatgttgt cggacttcat cctgtccagc gtcgcgccga gctcggcaaa
     8281 atagcttgcc tcgacgttca ggctcttggc caccttgatg ccgccgagaa actcggagac
     8341 cgtgcggtac tggtcctggc ggttgccggt cagcgtccgg ccataggcgc tggcgcgtgc
     8401 cctgaacggc tgcaaggcga tcagcagcac caggccgatg aaaatggcaa agcatgtcat
     8461 caccggcgag atgaagagcg agaccacgag atagccggtg agaagcagcg tggcctgggt
     8521 gagcatcagc agcgagaagg ccgcgacctg tacccggtcg acatcgccgt tcagcgcatg
     8581 gtcgaggtcg gagctgcgga tgcgggtgaa cacgccccag cgtgccttgc cgacgctctc
     8641 gaacaggttc atgcgcacgc ggttgacgaa gtcgtagagc agccgcgcca tgtagaccga
     8701 cttgaagcgg ttgaaaaccg cttgcagcgt caccagcgcg acgagcaggg aaagcacggt
     8761 ggcgagcgac agcgttccgc cgggcaccag ccaatggaga gcctccggca cccgaaccgc
     8821 gtaattctgc tcggccttgc cgatcaggcg cagcagcgga acgagcagca ggatggaggc
     8881 gccttcggtg aggcttccca ggatcaggaa ggcaagcgcc gtcgccgtgc gccgcccgcc
     8941 gacatgcgcc atcatcgcgc cgaagccggc gacatcccgg aagaacgaca ggcgaagctt
     9001 cgaggaccag cccatgcctt ggctcacctt gtctcggacg ccaggtcggt ttcgatcgcg
     9061 gcgaccgcct cgtcgatccg ggcgagcccg gccggcactt cgaggcggct gaggccgacg
     9121 cgtccggcga cctgtgcgca ttggccgaga tgcacggagg cggaatcgcc cggcagcgcc
     9181 tgccggccga accgcgtgat gtaggagaat ttgatgaccg ccggcagcgc ggcggccccg
     9241 gtcagcggcg agatcgccgc ccgcgcgccg cgctcgagca catagaggcg cgacaccggc
     9301 actgcttcgc cggcaaaccc gtcgcgcagc cggtgctgcg ccttgtcgat ttgcggatgc
     9361 acttgcgggc gtacctccgc ttgctcgagg cgaatggcgc cggccgcatc cgcggcgagc
     9421 ttgagctgcg gaaatccggg caggatcatc ggccgcccgg gatcggataa gtccagcgcc
     9481 acgacgtcgt cggtgagcag gcggtggccg gcgcggatca tcgcgcccgc cgtcgtcgac
     9541 ttgccggcgc ccttgtcgcc catgaagatc acgctcttgc cgccgacggc gatggcgctg
     9601 gcatgcagga cgagcagccc gcgccgatgc agggcgagcg ccatcaccgg cccgagcagc
     9661 ggaaaggcaa gcagcgggtc gcccacgcct ggcgctggat cgacgtcgat ccgcttgccg
     9721 tcgctgatca tgaaggtgcc gaccgcctgc caggaaagat attggcgcgt ggactcgaac
     9781 cggaacgccg tggcgccctg cgatgcggcg tcggggaagt cgaccggccc gaccgcgatc
     9841 tccaggtccg gcgtcgccgg cgccgccggt tcgagttccg gcagggccac ttccgaacgg
     9901 acgatcagcc cgtaggcctt gtagaaatgg cggacccgtc cgcccggcgc cgccgtctcg
     9961 gtggcgggcc cgggccgcgc aagggacgcg atcggaaggt tcatgcaggg cttccttgga
    10021 cttgccgcag ccacaatgac agcgaaaccg agcgccagac atgctggacg tcgggcagcg
    10081 tcgcctgctc gggccggctg agcaggcgct catagaccgc gttcacttca ggcaggttga
    10141 catagggcgc gatccgctcg ctgtccgata tcagcagcct gtccagaagt tcgcgatggt
    10201 tgccgagcat gccattgacc agattggcgg tgaaatcgat cttgtcgcgc cgccattgca
    10261 ccgcaggcgg cagaatgcct tgcatggccc ggcgcagcac atggcggccg aaaccgtccc
    10321 tgagcttttc ctcgccgggc agcgccaagc agaattcgac gaggggcttg tcccagaacg
    10381 gataacgcgg ctcgacaccg aaattggcgg cggccttatc aagcacttcg aaggcgtgcg
    10441 ggaccagccc ggtcgacaga agccagcgat gcgtcagcgc ttcgctggcc gtgacggctg
    10501 cgggcatgta gccgctgcga tgaaagcgct cggcaagatc ggtgcgcctg gcaagctccg
    10561 gattgaccag gcccgtccag gcggcgcgcg cggtttgcgc cggcctgcgc cgcgccttgc
    10621 cgacgacgcg tctggccatc gacctcaccc gggcgatccg ccaggccggt ccgtagatgg
    10681 tcaggaactt gaaatagagc gagagcatgc cctcgccata ggtattggcg gcgctgcgca
    10741 gctcgcgcca cagctccagc cacctgccgg cattggcgag ttcatgaagg tgtccatggc
    10801 cctgcgagac gacctcgtcg ccgccgtgcc cgtcgagaag cactttgacg ccctttgcgc
    10861 cggcggtgcg gtaaaggtcg cgagtcagcg acaggccggg cgccaggaac gtgccttcct
    10921 gctcctccag aatgcgatcg aattcggcga agggcgcgta attgcccacc gcaatcagcg
    10981 tggcgtcgag cggattgtcg gcgaggaccg catcgatgaa cggcttctcg tccatcggcg
    11041 agcccttgtc gaagaccagt gagaaggtct tgagcttcgg attgcgcttc gccgccgcgc
    11101 gaacaccggc aacgcaagcg atcgaggagg aatcgagccc gccgctcagc atggcgccga
    11161 cggccggcgt gccgcgcatg cggttctcca ccgattgcgc aaagagatgc ctgaattcct
    11221 cggccgcgtc cgagcgcggc ggccgttgcg aaggctcgat gtgccagtag cgccgcagcg
    11281 ccacctgtcg gtccgccacc atcatgctgt gccgtgccgg caggcggaag atctcggcat
    11341 agggcgtagc ctggggatcg tccggcagac cggcgaggaa gccggaaatc tggtgctcgc
    11401 tgaggcgcct gccgacgccg tcgagcgcca ggataggccc gatctccgag gcgaacgcaa
    11461 aacgcttgcc ggtcgcatga tagtagaaag gtttgacgcc gaaatggtcg cgcgcgcaaa
    11521 acagcgcctg gcgttcgcca tcccatatcg cgaaagcgaa atcgccctgc aggtagatcg
    11581 ggcaggcctc gccccatttg aggtaggcgc gcatgatgag cgccgcgtcg gccatcgacc
    11641 ggtcgcgcag gccgagccgc gccagaagct cgtcgcgatt gtccagccgg cagtcagcgg
    11701 tgatggcgag cttgccgccg gccatcgtca gcgggccgtg gccgtcctcg ccggtcgtat
    11761 cgagccaggc gtggccgagc gccacgctgc cctcgctcca ccaagaactg ccgtcgcggg
    11821 cgcggtggcg catgcgcgaa agcatctcct ggatatcgcc ggccccggcg ggccggccat
    11881 tgtcgtcatg cacgattccg gcgacgccgc tcattgctca cgacccatgg tcgaccagat
    11941 gggcaaagcc gttgatcgaa ccgccgagca cgatgtggtt gccgctcatc agccacgcat
    12001 gcgccttgag cgtgctgccg gtgcctttct cgatgccgat gctgatcttc gaggcattgc
    12061 cctggcgggc gagcagatat tgcccggcca gcgcctgggt gaggcacgtg gcgcccggca
    12121 cgagccgcgc cgctgcggcg acgccccagg cgacacgccg cagatcggcg acgctggcgt
    12181 cgcgcctggc atccagccgc gtgaccgcgc cgcggacacg gttgtaggag gacagcgtca
    12241 gcgcgagccg cactgcgctc accaccacca ggcagcgcgc caggaacagc atctcccggc
    12301 cgctcagcga gcggagccta gaccagttcc tcacgatgga gcctcgcaag ccctgcttcg
    12361 ctcagccccc tgagcaaacc ctcgacgtcc gccttgcagc gctccgcatc gacattgtag
    12421 cgcgcaagca cggcgctgcg gatatcgagg atcgatcgcg gctcctggat ctgctcccag
    12481 atgaaggcgc cgacgccgtt caggctgtaa tagacgttgg atttgagatg gagcagcgcc
    12541 agtccggggc cgaattcaca cgcgaccgcc tcgccggtcg caaccacgcg gtcatggtcc
    12601 gaggggttcc agtccatgct aaaacctcgt tgctggcccc gcgcccttgg gcctttcgcc
    12661 gtggatcggt tgccgacgat cctcggcagg aagccggggg acagaagccc cccggctcac
    12721 ctggtggaga gcatccggag ccccaactta gacacccggt tgcctgtgag ctttagcttt
    12781 aggagaaggt cagctcgccg accggcgtgt gcgcggggaa gctcgcgtca agcgccgtcg
    12841 tgccggcacc gccctgcgtg atcgtttcaa tcgaaccgtg aaccgtcagg ctgggggttt
    12901 cgtactcatg cttttcaaca ttctgttcca ttttactctc caactgagga ctggaagcgg
    12961 ctgcagaatg ccgccagcat atcgctatgc tgcaacgcaa ttgctgcacg gcaccattca
    13021 tgagtcaagc gtgatttacc aaccgttaat caattctcgg ttgcgctcgc cttccttggc
    13081 ggtcaagccg tgagcgccgt tagggtaaac cccgaccgcc ctcggattca accgcaggtc
    13141 tctgccgttt aggtaagctt ttgatttatt tgtggtaatt ttcacggcat gcaacccgtt
    13201 tcgcgcatgc gaagaaattg ctgcaaaaaa tcttcactat ctggcctcgg ccggatatcg
    13261 cctgtgttgt cttcaaaagt gattgaacac gtgacgattc taactcgtag gttgcagcga
    13321 tgaaccagct cgaacggggc tttaaagcag ctgcgttaat tttcccgatg cttttcttcg
    13381 cagttgcggt ataaatcgct cgggcggcat tttgcggaga acgacatcgg gcatggcggt
    13441 gcaggatggc tcctatgaaa ggctgcgcgc cgccggctgc gccaacgaag tcgcctatgt
    13501 gcaggcctgc ctgaggctgt ttttcacgcc ccgagccaag atcacggatg gcccagccga
    13561 tctgcccgca ctgacggtct cggcggcccc gatttcggcg gccccagtct cggcggccaa
    13621 cgtcgccgac atcgccaggc tgaacaaggt cgccgtgttc ctgctcaagg cgctcaagca
    13681 tgcgcccgcg gacgctgtgc ccgcggagtt ggctggctgg ctcgatggct accgtcggcg
    13741 cacgatgtcg atgaacgccg cctgcatcgg cgattcgata gccatcgatc gcgcgctgcg
    13801 cgagagcggg gtcgatttcg tcttcctcaa ggggccgctc cagcagcatc tgctctatgg
    13861 cgaccatttc atgaagcctt ctggcgatgt cgacatactg gtttcgccgg cgcgcttcgg
    13921 gcaagcgcgc gaggcgttgc gggcgattgg ctacgaggtt gccggcaaat cccgctccgt
    13981 ctggtggatt cgcttcctcg gcgagcagca catggttcgc gaagacggcg cgagggcctc
    14041 gacggtcgac ctccatcatc ggctgcagca gcctggctcg ccgagcccac gcgacatcga
    14101 cggcttcctg cgccgcaagc gcgaagtcga ggtggccggc gccgccatgc cggtgatctc
    14161 ggccgccgac acgctgctat tgtcctcgat cagcgtcgcc aaggcctttt tcaaccgcga
    14221 gccctgtgcc ggctatgtct gcgacatcag ggccagcgcc ggccgcctga acgaagccga
    14281 gcagcagagg gtgctcgatc acgccgccgg acagggcctg gcggacacgc tgctcctcgg
    14341 cctgcgcgcg gccgatgtgc tgctcggcgc cacaggcacg ctgctttcgg agcgcgccac
    14401 ccgcattctg gcccgcatcg acaacgccga tctcttccac atggtcatgg cgccgtggct
    14461 cgcgtcattg cgctggccgc agcgccgcca tgtgctttgg gagctctgcg gccgcgagcc
    14521 ggtccgttac ctggctgaag ccggttgggc cgcctccgcc gatctcagcc ggcgcatctt
    14581 cgagcggccg gccacgatcg ggccggcgca gggcgaaatg atcaaaatca tgggcggggc
    14641 tgccaatcat gcaagcgaaa ggtgagccca tgcccgtgac gacgcccatc accaatccag
    14701 gggtctgcgt catcatcgcc gcgcgcaatg cggcgcgcac cattcccggc gccatcgctt
    14761 cggcgctgcg cgaggcggaa gtcgccgaag tcatcgtggt cgacgacgcc tccaccgacg
    14821 acacccggga agtggccctg gccgccgatg acggcagcgg ccggcttgcc gtcatccgct
    14881 tcgacgtcaa tcgcggccct tccgccgcgc gcaacgcggc catccaagcc tccagatcgc
    14941 ccttcatcag cattcttgat gccgacgatt tcttcctcga gggccgcttt cgccggcttt
    15001 tcgccagcgc cgattgggat ttcgccgccg acaacatcat gctgatccga gacgatgcgg
    15061 cgacggacct cgccaagatc accgcgccgc ctttcgaagc cgagccggaa tttctcgatt
    15121 tcgagcgctt catcgacggc aacatctcgc gccgccgcgt gcggcgcggc gagttgggct
    15181 tcctcaagcc ggtgatcagc cggactttcc tcgaccggca tgggctgagc tacgacgaga
    15241 acctcaggct tggcgaagat tacgaactct acgcgcgcgc cgtcgcctgc ggggcacgct
    15301 tcaaagtcat caagtcctgc ggctatggcg cgatcgtgcg cgccgattcg ctgagcggcc
    15361 gccacagaac gcaggatttg aagcgccttg ccgatgccga tctggcgctg ctccagatcg
    15421 acagccttcc ggagcgctcg aaggcagcgt tgcggcgcca tgagcgccat gtccgcgaca
    15481 aataccggct gcgcaatttc ctcgatgtga aagccgagcg cgggctggcg tcggctgcgg
    15541 cttacgcctt cgccagccag tcgaatctga tcccgatcgt gcgcggcgtc gccaccgaca
    15601 agctcgacgc gctgttccgc cgcaccggga ttgttgcgcg gcagcaaata ccgccgatgc
    15661 gcttcctgat ggcggcaacc agcgccgcga acgagtgatc ctgcattgtc ccggaagctg
    15721 gcgcccctca aaaaatccgc attcggaaaa ctatgatgtt gcctagtcgc tgcgggaatg
    15781 gcagtctatg ttgcaatgca gcaatttcga aagaccgata tgatggcttc gatttttggc
    15841 ttcagatctc gggatccggc gcgcgaccgg cagaccgatc tccagcgttt cgaccggttg
    15901 gcgaagctct tcgaccagat cgcggcggag atcgaggcgg agaaaaccgg gctggagaac
    15961 cgttacaagt cgacggccgc caacgcggcc ttcctggtcg aggcgatgga gaacggttcg
    16021 gcatcggcca gcaagggatc ggacgtcagc gcgatgacca gctcgatcct gaattgcgag
    16081 cgccgcattg cggaactcgc tcgccagaaa ggtttgatga aggaactgcg tcattcgctt
    16141 gacgcgatcg tcggggatgg tttcgatcgg cccgctgcgc aggcgacggt aagacgttga
    16201 tgggcgcgtt gggcgctgtc atcggtacga ggcattagag gagttcaaag ggcggaggac
    16261 aggaagcggc tcgctcatgc gaaccgagga tgacttgggt tgggcggaca acacaaggtg
    16321 agtttgttat gaacgcagat ccggacaaca cctccgccgt ggcggcttgc gcgacgtcgc
    16381 atgcgccatg gagcggatta accagcctgc ggctaggctt cgtcggcgcc ttgcttgcga
    16441 tcgcagcgac ccttgccggg agcctgcatc ccgcgcatgc gcaggaggcg cagcaagagc
    16501 tgaaaagcgc accgtccttc gtcgacgatt tctcgaattt caaccagtct cgctggttcg
    16561 tctcggacgg ctggaccaac ggcgcccatc agaactgcac ttggtcgaag gatctcgtgc
    16621 ggctttccga cggcgtgctg tcgctgggct tcgaaaagcg caagctgaag gaccgcgatt
    16681 tcgcctgcgc cgagatccag acgaagcagc gctacggcta tggcgtctac gaggcgcgca
    16741 tgaagaccgg caccggctcc ggtctcaacg ccgccttctt ctcttatatc ggcccgcagg
    16801 acaaacagcc ctgggacgag atcgacttcg aggtcctcac caaggatccg tcgaaggtgc
    16861 aggtcaacag ctacatccag ggcaagccca agaacggcaa gctggtcgac gtcgagggcg
    16921 gcgccgacaa gggcttcaac gattatggct tcgtctggga gaaggaccgg ctgcgctggt
    16981 acatcaacgg caagctcgtc catgaggtga ccaacccgga cgagttgccg acccactcgc
    17041 agaaaatctt cttcagcctc tggggcagcg acaagctgac caactggatg ggcgccttca
    17101 ccgatccggg tagcagggtg atcatgcagg tcaagcgcgt cgctttcacc gctttgggcc
    17161 agccatgcca gtttccggaa tcgctggcat gcagcgtaag taacagcaac tagggcgtga
    17221 tcccgaaccg aaggacagcg tcagcaaaag tggcaatcgg tttttgcttc gctgacctgc
    17281 ggttcggata agatcatgct gaaccaacca ggccgttggc ctaggggaca tctgaaccgg
    17341 ccgggctgct tttgtggccc ggcctttgac aggaatagaa ccaaatgaat ttgacggtgt
    17401 ttgggattgg ctatgtcggt ctcgtccagg cggctgtgct tgccgaggtc ggccacgagg
    17461 tggtgtgcgt cgatgtcgac gagaagaagg tggagcggct caaccagggt ttgatcccga
    17521 ttttcgaacc cggcctggaa agtctcgtca aggaaaacca cgcggccggc cgcatccgct
    17581 tcaccacgga tgccgcggcg gccgtcaggc atggccagat tcagatgatt gcggtcggca
    17641 cgccgcccgg cgaggatggc tcggccgatc tgagatatgt gctcgccgtc gccgagacca
    17701 tcggtcgcga gatggacggc cccaagatcg tcgtcggcaa gtctacggtg ccggtcggca
    17761 cttgcgagaa ggtgaaggcc aagatcgccg agacgctgaa gaagcgcggc cgcgaggatc
    17821 tcgccttcga cgtcgcctca aatccggagt tcctcaagga gggctcggcg gtcgccgact
    17881 gcatgaagcc ggaccgcatc atcgtgggca cctccagcga ggacaccgag atggtgatgc
    17941 gcgagctcta tgcgccgttc aatcgcaacc acgagaagat gatcgtgatg gatgtacgca
    18001 gtgccgaatt caccaaatac gccgccaatt gcatgctggc gaccaagatc agcttcatga
    18061 acgagatggc caacctggcc gagcagctgg gcgccgacat cgaggaggtg cgcaagggca
    18121 tcggctcgga tccgcgcatc ggctaccact tcatctatcc cggcctcggc tatggcggct
    18181 cctgcttccc gaaggatgtg cgcgcgctga tcaagaccgc tgagggcgtc aagttcgacg
    18241 ccaagctgct gcgcgccgtc gaggagcgca acaatgcgca aaagtcggtc ctgttcgata
    18301 aggtcaacca ctatttcaag ggcgcgctca ggggcaagac cttcgccgtc tgggggctgg
    18361 ccttcaagcc aaacaccgac gacatgcgcg aggcgccgtc gcgcacgctg atggaagcgc
    18421 tgtgggcggc cggcgccaag gtgcaggcct acgatcccga ggcgatgcag gagtgccagg
    18481 cgatctatgg cctgcgcgag gatctgcttt tgtgcggcac caaggaggcg gcgctgcgcg
    18541 gcgccaacgc gctgctgatc tgcaccgaat ggaagagctt ccgcgcgccg tccttcgacg
    18601 cgctcaagga tgcgctgacg acgccggtca tcttcgatgg ccgcaatctc tacgatccca
    18661 aggtgatcgc gcgctacggc atcgagtatt tctccatcgg caggatggcg gcatgacgat
    18721 ggctgcttgc cccaggaaga acttccgccc cgaacgacag cggggccgac aatgaccatg
    18781 cccgtgaagc aggagggttc gatctgatgg tgctggacgt tcggcccgaa caaatcgcca
    18841 gcgagcattt cgatctcgtc gtcatcggat ccggcttcgg gtcgtccttc ttcctgcacg
    18901 aattcctgaa gcggcgcaaa gcccgcgtcc tcgtgctcga atggggccgc cacaatacgc
    18961 atgagtggca gctcagccag aacgccaaca ccgacataga cgacgagagc acctacgata
    19021 cggacaatcc cgacaagccg tggaattaca cgatcggcct cggcggcggc accaattgct
    19081 ggttcgcgca gacgccgcgt ttccatccaa acgacttcag gctgaagagc acctatggcg
    19141 tcggcaacga ctggccgatc agctatgacg agcttgagcc gttctatggc gacgccgagg
    19201 aaatcatgtc gatctccggc gatcccgaca tggcgcggat gctgccgcgc tcgcgaccgt
    19261 ttccgcagcc gccgcatcgc atgtcgacgc ccgaccgtat gatgaaggcg gcgcagccgg
    19321 atcagcactt cgtcatgccg accgcccggg cgcgggtggc gacgccgcag cgcgcttcgt
    19381 gctgcgccaa tttgagatgc tggttgtgtc cggcggatgc caagttcacc gtcaacaacg
    19441 gcctgatgca tgtcttccag catcccgacg tctcggtttg cctcggcgct gaggtacgcc
    19501 ggttcgacca tgccggcggt gcggtccgct ccgtcgcctt cgtgcgggac ggcaaggaat
    19561 attcagtcgg cggcgatctc ttcatccttg gcgccaatgc catacagagc cccgcgatca
    19621 tgctgcgctc cggacttggc ggcgagttcg tcggcttggg cctgcacgaa tcctatggct
    19681 ggaattacga ggcctatctc ggcggcgtcg acaatttcga cggctcgacc atcaccaccg
    19741 gcctcaattt cggcctttac gacgggccgc accgggccga gcatgcggcg gcgctcgtct
    19801 atttcgagaa tcgctggcag cacggtatgc gcgccgagaa gggcaggctg cggcaggtgc
    19861 tgccgctggt catcgtcacc gagaacctgc tcgatcctga aaaccgcgtc atcctcgaca
    19921 agaacgagaa cgccttcgtc agcttcaagg gcgcgtctga ctatgccacc aagggcatgg
    19981 cgaaagcgct ggaaaggctg ccggagctgc tcaagccgct gccggtcgag cagattttcg
    20041 atcgcggcat aaggccgacg gaatctcacg tgcagggtac gctgcgcatg ggcacaggcc
    20101 cggccgattc cgtggtcgac cgcgacatga tccatcacca gctcaggaac ctcgtggtcg
    20161 tcggcaccag cacctatccg agctgttcct gcgccaatcc aagcctgacc gcggctgccc
    20221 tgtccttgcg ggcggcgggg aagctggcgg catgagggag gggccgatga agatcacacg
    20281 ccgcaccgca ctcgccatcg tcgccggcgg cattgcctcg acgggtatcg ccgctgccgc
    20341 cgtctcgtcc ttctcggacg aggacctggt tcgcgtcacg ctggagaaat atttcggtcc
    20401 gctcaacatg cgcatcgagc acctgcagca attcgtgctc gagtttcaga accgcaaccc
    20461 ctggatattc ccgagcgcca aggtggcgga cgtggtgacg ctggccgaaa cggtgaagct
    20521 cggcaaggcg cgcgagctgc tgccgcagga caaacggcgc gatctgcgcc acttcgatcg
    20581 ctgggtcatc gccgagttcc acatgctgac cgattacgcc tggcgcagct cgcccgacga
    20641 tccggtccgc ttcaccggct ggcatccatg caccaatccc ttcgccgatc tggagctgca
    20701 gaatgcgtaa ggcaggcgga acggaagtcc tttgttttca cgcaattccg gacgcaaaac
    20761 cgtctaatgg ttttcctgga attgcttcag catgaggtcg caaatggctg tgcagcgcaa
    20821 accgacggtc accgtcatcg tccccacctt caatcgtgaa aaattcctgc ccgaagccat
    20881 cgacagcctg ctgcggcaga ctgtggcgcc ggatcagata ctaatcgtcg atgatggctc
    20941 gacggacaac accgccgcgg tagcggccgc tttcccggcg ccggtggaat attaccgcaa
    21001 ggagaatggc ggcaagtcga cggcgctgaa tttcgccctg aagcactgca cgggcgattt
    21061 cgtctggata ttcgacgacg acgacgtcgc gcacccgacg gcgctggaac ggtttctcgc
    21121 cgccttcgag cgggaaccgg acgccgattt cgctttcggc gaatatgcgc gcttccagcg
    21181 ctcggcccag atcgaggagg aggactacga gatcgtcgcc ttcggccacg tcacccagga
    21241 caatttcttc ccgtcccacc tcgaatattg ccacgtccac caacccggtc tcctggtgcg
    21301 ccgcgaatgc tatgacgagg tgggaggctt caacgagacc ctggtccggt cgcaggacta
    21361 tgagatgatg ctgaggctcg ctcgccggtt caaggcaatc cgggtcgagg gcctgatgtt
    21421 cttccagcgc cagcacgaca tggtccgcgg cagcaccaag gtcgtgattt cctataccga
    21481 ccggataaag gcctggggct cgtacgaccg cgtgatcatg gaggagatct acaagtcggt
    21541 cgatctccac gaatatctcg atcgctcgga acaggccttg ccgaagacgg ccgatctcga
    21601 gatgcaggcg ctgctgcagc gctcgagcat catggccagg aagggcctat ggcaccacgc
    21661 ggccgcggac atccgccgct acgcccagct tgccgagcga gccggaaaga cctcgctcag
    21721 cgacaaggag cgcgccatcg tgcggcgcac cttcgaagcc tatgacacgg gcctggcgtc
    21781 cgccaccgcc gacgcattcg tctcggcggt gaaaggatgg aaggcgggtc cgctgaaggc
    21841 cgacatcgtc agggagtaca tgcactcttt cccctatttc ctcgcccgcg atttgaaggc
    21901 gggaaaactc gttccagcgt cgaagctttt gcgggagtac tcgacgctgt ctctttcagg
    21961 ctcggtcagt cgcctctgtc gggcaatgac gtcgaagctt ccgttgcagc gcggcgcaag
    22021 ctgacggcct acaattgagc gccgggctgc cggacaacgc cggaccgaat taggagccga
    22081 acgatgaaag tgcttttcgc aagcggcaac ggatacatcc ccgagttcac cggcggcgtg
    22141 caatccaaca cccatcatct ggtcgaacag ctgatcgagc atggccacca ggcgtcggtc
    22201 ctggcctcgc tgttcggcga cggcatgttc ggcttcaagg cgcgcgccaa gatgaagctg
    22261 ctgcggcagc cggcggtggt cgacaatttt ccgggctacc cggtgatacg cgcctggttc
    22321 ccctgggacg cggccggctt cgccgtcagc aaaaccaggc ccgacgtcgc cgtcgtgcag
    22381 tgccacaaat ccgtcccgct cggaaaggcg ctcctcgccg aaggcgtgcc gctggtcgtc
    22441 tatctccaca atgtcgaatt ccacgagctc gccggcgatc cgcgcgagct cggttcggcc
    22501 ttgtacatcg ccaattcgga attcaccgcg cgcacctaca gggaaaaatt cggcatcgaa
    22561 tccatcgtgc ttccgccgac catcaacgac gccctctatt gcacgcacac gaccggc
//
