LOCUS       NZ_JADDUE010000019     22454 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Saccharopolyspora sp. HNM0986
            NODE_19_length_56274_cov_77.990615_cov_77.990615, whole genome
            shotgun sequence.
ACCESSION   NZ_JADDUE010000019
VERSION     NZ_JADDUE010000019.1
KEYWORDS    WGS; RefSeq.
SOURCE      Saccharopolyspora sp. HNM0986
  ORGANISM  Saccharopolyspora sp. HNM0986
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Saccharopolyspora.
REFERENCE   1  (bases 1 to 56274)
  AUTHORS   Li,F.
  TITLE     Diversity and distribution of actinomycetes associated with coral in
            the coast of Hainan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 56274)
  AUTHORS   Li,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2020) chool of Life and Pharmaceutical Sciences,
            Hainan University, 58 Renmin Avenue, Meilan District, Haikou, Hainan
            570208, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016526145.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 02:15:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,899
            CDSs (total)                      :: 5,838
            Genes (coding)                    :: 5,784
            CDSs (with protein)               :: 5,784
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 7 of 54
            Pseudo Genes (incomplete)         :: 49 of 54
            Pseudo Genes (internal stop)      :: 0 of 54
            Pseudo Genes (multiple problems)  :: 2 of 54
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:09:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 11863
            Orig. end                         :: 34317
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADDUE010000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22454
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22454
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22454
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12454](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12454
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    complement(42..56)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.51"
                     /tool="antismash"
     misc_feature    complement(110..123)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.91"
                     /tool="antismash"
     gene            207..812
                     /locus_tag="INP57_RS28125"
                     /old_locus_tag="INP57_28140"
     CDS             207..812
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640743.1"
                     /locus_tag="INP57_RS28125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28140"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_200073397.1"
                     /transl_table=11
                     /translation="MDECPIDREVSTEQLLVKIGSGDLRAFHTFYDRTVRPVRTLAREL
                     LDDDAQAEEVAQEVLIEVWRTAPRYRPERGSAQSWVSTMTRRRSVDRLRSARAVRERDD
                     RFAAGSQETAYDSVTETVLAHCEHLKVRSCLSALTALQSEAVLLTYYRGLSYREAAEQL
                     RIPPSTVKTRLRDGLLRLRECLGDCCLASPGQAREDRP"
     gene            1043..1348
                     /locus_tag="INP57_RS28130"
                     /old_locus_tag="INP57_28145"
     CDS             1043..1348
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="INP57_RS28130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="INP57_28145"
                     /product="hypothetical protein"
                     /protein_id="WP_200073398.1"
                     /transl_table=11
                     /translation="MRKLNRGIAAGLISAPLVLGFAGVAGAADFEGTQSVAGPEGVQSS
                     QTTAGADSNGDGRVSYEESHSVAGPDGAATQQTSSSSDSGSGDSGSDGGLLGILGL"
     gene            complement(1452..2360)
                     /gene="rfbD"
                     /locus_tag="INP57_RS28135"
                     /old_locus_tag="INP57_28150"
     CDS             complement(1452..2360)
                     /EC_number="1.1.1.133"
                     /GO_function="GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (63-163). E-value:
                     4.4e-11. Score: 34.3. Matches aSDomain:
                     nrpspksdomains_INP57_RS28135_NAD_binding_4.1"
                     /codon_start=1
                     /gene="rfbD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948706.1"
                     /locus_tag="INP57_RS28135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28150"
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="WP_200073399.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.8e-105,
                     bitscore: 343.9, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAQRLALLVPGGRGQLGRELARSAVPLVHAPGSGELDLRDAAEV
                     GDAVGSFAETALDNDLRPVVVNAAAYTAVDAAESDADAARSINVDGAAALARACAARRV
                     PLVHMSTDYVFSGDADVPYEPDDPAEPRTVYGRTKLDGERAVLSAGARACVVRTAWVYG
                     AGGQNFVRTMSRLERERDSLSVVDDQVGAPTWTGDLAAGVVQLAGSFAERRDPQRPVLH
                     CTNAGRTSWFSFARAVFAELGADPERVQPCSTEEFPLPAPRPAHSVLSGRAWAAEGLTP
                     LRDWRSALTAAFETDGAALRR"
     aSDomain        complement(1872..2171)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_INP57_RS28135_NAD_binding_4.1"
                     /evalue="4.40E-11"
                     /label="INP57_RS28135_NAD_binding_4.1"
                     /locus_tag="INP57_RS28135"
                     /protein_end="163"
                     /protein_start="63"
                     /score="34.3"
                     /tool="antismash"
                     /translation="VVVNAAAYTAVDAAESDADAARSINVDGAAALARACAARRVPLVH
                     MSTDYVFSGDADVPYEPDDPAEPRTVYGRTKLDGERAVLSAGARACVVRTAWVYG"
     gene            complement(2357..3337)
                     /gene="rfbB"
                     /locus_tag="INP57_RS28140"
                     /old_locus_tag="INP57_28155"
     CDS             complement(2357..3337)
                     /EC_number="4.2.1.46"
                     /GO_function="GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /GO_process="GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (4-182). E-value: 1.6e-20.
                     Score: 65.2. Matches aSDomain:
                     nrpspksdomains_INP57_RS28140_NAD_binding_4.1"
                     /codon_start=1
                     /gene="rfbB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420983.1"
                     /locus_tag="INP57_RS28140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28155"
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="WP_200073433.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 7.9e-19, bitscore:
                     59.5, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 9.3e-22,
                     bitscore: 69.2, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 1.4e-20, bitscore:
                     65.2, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLITGGAGFIGSHYARQLLQGAYPAFADAEVVVLDKLTYAGNQ
                     ENLRPVAENPRLRFVRGDICDAELVAELMTGVDVVVHFAAESHVDRSILGASDFVRTNV
                     VGTDTLLQAALAAGVGKFVHVSTDEVYGSIETGSWPEEHALEPNSPYSAAKAGSDLMSR
                     AYFRTHELPVCVTRCSNNYGPYQFPEKLLPLFITNLLDGHRVPLYGDGGNVRDWLHVDD
                     HCRGIQLVAESGRPGEVYNIGGGTELTNRRLTERLLEAMGQDFSMVRPVDDRKGHDRRY
                     SVDHTKIAAELGYRPRVGFDEGLAETIRWYRENRSWWEPLKQVQK"
     aSDomain        complement(2792..3325)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_INP57_RS28140_NAD_binding_4.1"
                     /evalue="1.60E-20"
                     /label="INP57_RS28140_NAD_binding_4.1"
                     /locus_tag="INP57_RS28140"
                     /protein_end="182"
                     /protein_start="4"
                     /score="65.2"
                     /tool="antismash"
                     /translation="ITGGAGFIGSHYARQLLQGAYPAFADAEVVVLDKLTYAGNQENLR
                     PVAENPRLRFVRGDICDAELVAELMTGVDVVVHFAAESHVDRSILGASDFVRTNVVGTD
                     TLLQAALAAGVGKFVHVSTDEVYGSIETGSWPEEHALEPNSPYSAAKAGSDLMSRAYFR
                     THELPVCVTRCSNNY"
     CDS_motif       complement(3266..3328)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_INP57_RS28140_0001"
                     /evalue="1.20E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="INP57_RS28140"
                     /protein_end="24"
                     /protein_start="3"
                     /score="11.6"
                     /tool="antismash"
                     /translation="LITGGAGFIGSHYARQLLQGA"
     misc_feature    3498..3516
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.59"
                     /tool="antismash"
     misc_feature    3499..3515
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.72"
                     /tool="antismash"
     gene            3667..5433
                     /locus_tag="INP57_RS28145"
                     /old_locus_tag="INP57_28160"
     CDS             3667..5433
                     /GO_process="GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239653.1"
                     /locus_tag="INP57_RS28145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28160"
                     /product="LCP family protein"
                     /protein_id="WP_307850324.1"
                     /transl_table=11
                     /translation="MDDRSRRPGGPPGQPGRPVPPRGPQRQPRPGSGPAGRPAPGGGPR
                     QPSQDRPAPADRPARAQQRPPGEQQRRPEPRPERPAPPPPEDTWNRGRNARRNGMIVGR
                     TFVALLSVLVLAFTGYAWATLRNLNSNLSGSDVIGGGLSEPDGATDILLIGNDSRTDAD
                     GNPLPDEILKTLRTTDDEGGDLTDTMILIRIPNGGGRASAMSFPRDTMVQMGNGYGENK
                     LNSALPRAKSDAKQKLQASGVTDPKQLELQSKAEGQKFLIKTLEDLSGVSIDHYAEVNL
                     LGFYEITQAVGGVDVCLKDEVNDSDYSGAVFKAGPQTVAGADALAFVRQRHGLPRGDLD
                     RVVRQQVFMSGLARKILSSGTLSNPAKLTSLMEALKKSVVLDKGWDVVDFAQQMQGIAG
                     GDISFQTIPIELEGESGSEDVTANPREVKQFADGLLLPPQERAAKEQAEQAAEKQRGGT
                     TVNVFNATGKSGLAGQVLSTLSEAGFSQGGSSNSDSMAKSVVYHAPGDEAAAGQVAATL
                     GGLPLQPSGNLQSGNVEVYVGSDYQGPGAQQFAGNKQVQLDGGQRAAAPLHAQQSGSGD
                     APIDAGGVPCVN"
     gene            5542..7101
                     /locus_tag="INP57_RS30455"
                     /old_locus_tag="INP57_28165"
     CDS             5542..7101
                     /GO_process="GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948701.1"
                     /locus_tag="INP57_RS30455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28165"
                     /product="LCP family protein"
                     /protein_id="WP_307850325.1"
                     /transl_table=11
                     /translation="MAEEDVPTAGSGDSREPDEQRAAQPDHGRAADAAGGGSDPGDPAS
                     HGTASGRTAWHGTVWTGTVWPGDASGGTASRSAASDDTASRSAASDDTTSQSAASASAA
                     SRSTGSDGAAAADSSVLDGSEPDDAGGSERAAAAPRKHRGWLIAGRTLVALISAGVLAV
                     TAAGWVALDRLDTDVSTSRVLADTPEAPPADDGATDILLVGNDSRTDAQGNPLPLSVLR
                     ELRTESTNGLNTDTVILMRIPHDGGPPTAVSLPRDTSVPMPGGGTQKINAVYGLAKSAA
                     ADSIEGRDSALAERQAQLAGQRALLQSVQRLSGVRVDHYAEVNLLGFYEVTDALGGVEV
                     CLNQATSDKDSGADFAAGRQTISGGDALSFVRQRHGLPGGDLDRIVRQQVFMAAVANKL
                     LSTGTLTDPAKLAGLQDAARRSVVLDENWDVAGFAAQMQGLAGGGVRFVTLPVASTGGR
                     DEHGQSVVKVDPAQVRSFIAGLVPKTGFAANAPLDLDGTARRQPAPPAQDPITSDGIPC
                     IN"
     gene            7333..8052
                     /locus_tag="INP57_RS28155"
                     /old_locus_tag="INP57_28170"
     CDS             7333..8052
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796106.1"
                     /locus_tag="INP57_RS28155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28170"
                     /product="TIGR03089 family protein"
                     /protein_id="WP_200073400.1"
                     /transl_table=11
                     /translation="MSVTQALLAPMLATGSPRPLITHYDDATGARVELSRATVANWAAK
                     TANWLVDELDLEPGTPVSVALPAHWQTLGVLLGSWWCGAHVVDDPAGAEVAFVPAAAPE
                     SGAGAHVTAVVGLDALGGPVRELPQGAVDYVSDIRVHGDEFSPRQQVPGSAPALVGSTV
                     DEVLGVAAERAEALGIGPDSRVLSTLEWTLPGGVYDGLLAVLSARSSLVQVSNPDPDKL
                     AQRRTTEKTTVDLGAVL"
     gene            complement(8147..8566)
                     /locus_tag="INP57_RS28160"
                     /old_locus_tag="INP57_28175"
     CDS             complement(8147..8566)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019810444.1"
                     /locus_tag="INP57_RS28160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28175"
                     /product="DoxX family protein"
                     /protein_id="WP_200073401.1"
                     /transl_table=11
                     /translation="MHRYRDLTTLLARLVVGVTFVLHAYQKLAINGMSGVSAGFEQMGI
                     PLPAAAAWFTALVELLGGLALILGVLLPVAGVLLAAVMLGALVTAHSGAYFATDGGFEY
                     VLVLAAVSLALGFSGPKYTLQAAFAARKDSAPAAV"
     gene            8743..9525
                     /locus_tag="INP57_RS28165"
                     /old_locus_tag="INP57_28180"
     CDS             8743..9525
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF044908.2"
                     /locus_tag="INP57_RS28165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28180"
                     /product="thioesterase family protein"
                     /protein_id="WP_200073402.1"
                     /transl_table=11
                     /translation="MQDETLFRPAGELFLPTDSAGNPWGELTGGGPVAGLIARAVQREL
                     SDPDLFLARLTVDLHRPVPRRTGVAVHTRTVREGKRLRVLEVVLHQDGTEVTRALAHVL
                     RRDRTDEAEPDGEIPFAGPDGITDGTLLPAGLGLRWGVHDVIQVRWLSDQISGSPSRAW
                     MRLPLPLLPDEPTSPLTQLAVLVDCISAASPVGALFGPWINSDITLYLHREPRGEWIGM
                     EIERDVEPTGIGVARARLFDEHGPIGTAHEAVLEHQLG"
     gene            9869..9985
                     /locus_tag="INP57_RS28170"
                     /old_locus_tag="INP57_28185"
     CDS             9869..9985
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="INP57_RS28170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="INP57_28185"
                     /product="lasso RiPP family leader peptide-containing
                     protein"
                     /protein_id="WP_200073403.1"
                     /transl_table=11
                     /translation="MEEQAAPEPYEPPVLVEVGEFGADAAADGSGQFDASVR"
     gene            10001..11809
                     /locus_tag="INP57_RS28175"
                     /old_locus_tag="INP57_28190"
     CDS             10001..11809
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012936.5"
                     /locus_tag="INP57_RS28175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28190"
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_200073404.1"
                     /sec_met_domain="Asn_synthase (E-value: 4.5e-21, bitscore:
                     67.6, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTQRRDSGWVALPDAPEMRAVADRFDPHGRIVLPHASGNPWLVG
                     HFAPDELVPVALGPVRVALVGRCPIDAARLAELARAVHSVSDVDGIVRALPGGVHVVVS
                     VGGDVRVQGGISGLRQVFHGVREGFPIAGDRADLLAGLLGAGIDDRALAAQVSCAQLPP
                     PLAGQSIWSGIRAVPADSYLRLLGHRATEVRWWHPPEPEVPLRRGARFLLERLESAMET
                     GAGEAGRAGSELSGGMESAALCSLAAARNSDLLALRMGNGGRESAAPEWRSAEPLVLDD
                     AELPDMFAEPDAAADPEHPYPGWRTAARIRYSARILGERGVRRHFAAHGGEELFHDGPA
                     HLHALLRHRPVTALRRLRGYRAARNWALPPTISALLSAHDVGRWWHEQADRLTEPYPDS
                     NAPRLDWGAAPVRVAPWASAEAVSTTRALLHRTARKTERLAPDRATHQLLSALRTGAPA
                     HRRLARSYAAAGVRLELPFHDDRVVEAVLAVRPHERCSPWEDKPLLAEAMREVLPGALP
                     RRANTGDGEAIRIGFRRNRAAILALFADSELAQHDLIDTGVLRRHLLAPHVGEHTLFQL
                     DHLLGCENWLRSATRAGRTDVPATAS"
     aSDomain        11787..11969
                     /aSDomain="Lasso_Fused_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing fused peptidase in a lasso
                     peptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_INP57_RS28180_Lasso_Fused_RRE.1"
                     /evalue="4.00E-09"
                     /identifier="RREFam003.1"
                     /label="INP57_RS28180"
                     /locus_tag="INP57_RS28180"
                     /protein_end="61"
                     /protein_start="0"
                     /score="27.4"
                     /tool="antismash"
                     /translation="MSLRLRPDVATTDTAEGTVLLDERTGRSFQLNRTGTHVLRGLLLG
                     QDTEHIAQELAERHRI"
     aSDomain        11787..12038
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_INP57_RS28180_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="5.00E-23"
                     /identifier="RREFam002.1"
                     /label="INP57_RS28180"
                     /locus_tag="INP57_RS28180"
                     /protein_end="84"
                     /protein_start="0"
                     /score="71.9"
                     /tool="antismash"
                     /translation="MSLRLRPDVATTDTAEGTVLLDERTGRSFQLNRTGTHVLRGLLLG
                     QDTEHIAQELAERHRIPPHRAKRDVATIVERLRSVTLLE"
     gene            11787..12047
                     /locus_tag="INP57_RS28180"
                     /old_locus_tag="INP57_28195"
     CDS             11787..12047
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017239.5"
                     /locus_tag="INP57_RS28180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28195"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_200073405.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 9.4e-23,
                     bitscore: 71.9, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 7.7e-09,
                     bitscore: 27.4, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.9e-12, bitscore: 39.2,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLRLRPDVATTDTAEGTVLLDERTGRSFQLNRTGTHVLRGLLLG
                     QDTEHIAQELAERHRIPPHRAKRDVATIVERLRSVTLLESA"
     gene            12044..12454
                     /locus_tag="INP57_RS28185"
                     /old_locus_tag="INP57_28200"
     CDS             12044..12454
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005163726.1"
                     /locus_tag="INP57_RS28185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28200"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_200073406.1"
                     /sec_met_domain="PF13471 (E-value: 3.8e-23, bitscore: 73.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLPVTLPVRENLPLRQRASALLAVGLARLLVLLRPARQLKLLRF
                     ARRRAVPASATQALAARRDVLAVSPRSADCPLRRSIATALLCRARGVWPTWCTGVRTAP
                     FAAHAWIEVDGHPVGEPHASAHYRRLQSIPPR"
     misc_feature    12131..12133
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(12474..12989)
                     /gene="purE"
                     /locus_tag="INP57_RS28190"
                     /old_locus_tag="INP57_28205"
     CDS             complement(12474..12989)
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /GO_component="GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA]"
                     /GO_function="GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA]"
                     /GO_process="GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /GO_process="GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="purE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443592.1"
                     /locus_tag="INP57_RS28190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28205"
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="WP_200073407.1"
                     /transl_table=11
                     /translation="MVQPLVGVIMGSDSDWPVLQAAGEALSEFDVPYEVGVYSAHRTPQ
                     RMLDYAREAADRGLRAIIAGAGGAAHLPGMVAAATVLPVIGVPVPLKHLDGMDSLLSIV
                     QMPAGVPVATVSVGGARNAGLLAVRTLAADSGELGTRLRTAMARFQSDLESMVHDKHAA
                     LQSRATEQ"
     gene            complement(13099..14283)
                     /locus_tag="INP57_RS28195"
                     /old_locus_tag="INP57_28210"
     CDS             complement(13099..14283)
                     /EC_number="6.3.4.18"
                     /GO_function="GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948695.1"
                     /locus_tag="INP57_RS28195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28210"
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="WP_200073408.1"
                     /sec_met_domain="ATP-grasp (E-value: 1.4e-53, bitscore:
                     172.7, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDHRTRTPVVGMVGGGQLARMTHQAAIALGQSLKVLAAAPDDPAA
                     LVAPDVQIGPHTDLAALRAFAQGCDAVTFDHEHVPGEHLRELVAAGAAVHPGPDALLHA
                     QDKLVMRRKLAEMGLPVPPFAEIRSVSDALEFGAEHGWPCVLKTARGGYDGRGVWTLNT
                     PESAQRTVTELLEAGAVLLAEQRVPLRRELAALVARSPFGQGAAWPLVETVQSEGICVQ
                     VLAPAPDASEQLREQAQNMALQIAEELGVVGVLAVELFETADGLVINELAMRPHNSGHW
                     SMEGALTSQFEQHVRAVLDYPLGSTGLTAPAVVMANVLGAPTEPKMGVDERMHHLFARF
                     PHAKVHLYGKAERPGRKIGHVNVLGEDMSAVRREADLAAHFLSHGEWADGHLVH"
     gene            complement(14383..15183)
                     /locus_tag="INP57_RS28200"
                     /old_locus_tag="INP57_28215"
     CDS             complement(14383..15183)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315646.1"
                     /locus_tag="INP57_RS28200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28215"
                     /product="hypothetical protein"
                     /protein_id="WP_233628525.1"
                     /transl_table=11
                     /translation="MTERIRSAAGGERALRARWRTASLAAGWPFPSDWGIRAVREVCRA
                     VATGAELAEPLGELGAGRAESGVGLAEALQDVAALHAVSTGSHQDGLVSADPDAVPAAM
                     LRPVALGWADVQAEHTVDREVQDPLTGLTTASYLRTRLHEVYRGARAAGRSPDADHVLI
                     SVSVQMPRASGRYPLMMAMVLAADVLREVFDSGETVSLLRTSTAAVLAPRDERFSERCE
                     QVRLQAAQRIAADPALRPVGPVRVQEEPLPEDHATACAMLGTLD"
     gene            15456..17876
                     /locus_tag="INP57_RS28205"
                     /old_locus_tag="INP57_28220"
     CDS             15456..17876
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875055.1"
                     /locus_tag="INP57_RS28205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28220"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_200073409.1"
                     /transl_table=11
                     /translation="MATAPAEAQDPSDGELIEAVRHGSTEAYGTLYERHVAAAHNMARQ
                     VARSGSEADDLVSEAFAKVLDALRGGHGPSTAFRAYLLTSLRHVAYDRTRRERKLQLAD
                     DVAEVSGADLSVPFNDTAVAGLERSLAARAFTQLPERWQTVLWHIEIEGQTPAQVGPIL
                     GLTPNGVSALAYRAREGLRQAYLQVHLSSLDPEQPGLQACRAAVGRLGAWTRGGLSRRE
                     TAQVDHHLDDCDRCRALAAELAEVNGGLRAVVAPLVLGAGAAAYLAAAPAAGSVSAAGA
                     GAAGAANAASSVPRQAISSGAAATALVLTVLLALTSGTASLGPVASDPPAPQAVLPQPP
                     APKPAPPQPQRAPQPQPEAPAPPPAIPDLHATGPAQPLELVPGGPAVELPITVTNRGQM
                     TSEPVTATLSLPPGVSAEVPGATTQASGPAGTAPASSTAPASTTPASTTPASAAPAGTA
                     ASSAGPRSTAPAGSGSAGIATARAAPASTGPASTTPASARSAAPAGEPQVRCQAYEGGV
                     RCTSDRGVPPGGHLTFRLRVQAAPNASEGEIRAQITSGATIDLQLSAIRVRLAPADGVD
                     VRVSRAAFDEGLSEQVDDHPPVHLELDVRNTGRTAGRAEAMARLPEGTRAIGIPPECEV
                     HPSERQLRCAKDLRPGESFEGDLWLLTFQDAPVADAERQVTIPAQATLGQASDSDSLTL
                     RLRYPGVPPKPCPGIPEFPGQGWWHCWWPEPPESPEPPEPPEPPEPPQPPEPPRPPESP
                     QHPEPPEPPELPDLLQPPDDPPQQETSRPEHDEPDAPSGTRPWPWPLRDLSGR"
     gene            18001..18501
                     /locus_tag="INP57_RS28210"
                     /old_locus_tag="INP57_28225"
     CDS             18001..18501
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677282.1"
                     /locus_tag="INP57_RS28210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28225"
                     /product="GtrA family protein"
                     /protein_id="WP_200073410.1"
                     /transl_table=11
                     /translation="MSVVDSVLTRIPQPYRNVAIRHRELLKFAVVGAITFFVDTAIFYG
                     LKLTILVPKPVTAKVIAVLVATIASYVLNREWSFRTRGGRERHHEAALYFLFSGIGVAL
                     YAAPLWFSRYALHLQEPYTSRFVEEIADFTAAQIVGLLLGMAFRWWAFRRWVFPSGDSE
                     QDE"
     gene            complement(18542..19816)
                     /locus_tag="INP57_RS28215"
                     /old_locus_tag="INP57_28230"
     CDS             complement(18542..19816)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875056.1"
                     /locus_tag="INP57_RS28215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28230"
                     /product="ATP-binding protein"
                     /protein_id="WP_200073411.1"
                     /transl_table=11
                     /translation="MRRRILAATLLAVAVTAVVLGLPLGVTALKLVEDFTRADLSTRSQ
                     QIATSLDEQVASHRPIDVESVLLAVPRGAQLVVRTKQQDYVYGSDPGPSPLTERVPMVQ
                     SGTVTLSAPSAPVRTQQFQVAGLVLLLVVISAGTGMVVAGLTARRLADPLRHVAARAAR
                     LGAGDFRADPKRHEVPELDRVADALDASGAALSHLVQRERELVGDVSHQLRSRLTALQL
                     RLEALAEVPDQEVSEEATAALEQAERLSSVLDDLLAAATAARARDAEPLDVSALLAEAA
                     TEWRDLLRAQSRTLRQRVPEGLLARATPARLREAIGVLLDNALRHGKGAVTLAAREGGG
                     TVVVEVGDAGPGVPDQLVPYVFDRGFSAGGSTGVGLALARALIEADGGRLELSKARPAL
                     FEIYLPVARADDVLGVPWKIESTPR"
     gene            complement(19819..20493)
                     /locus_tag="INP57_RS28220"
                     /old_locus_tag="INP57_28235"
     CDS             complement(19819..20493)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986855.1"
                     /locus_tag="INP57_RS28220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28235"
                     /product="response regulator transcription factor"
                     /protein_id="WP_200073412.1"
                     /transl_table=11
                     /translation="MSVVLLAEDDPAIAVPLSRALQREGYSVRVIEDGPAALREASSGV
                     IDLMVLDLGLPEMDGLEVCRRLRAQGRGIPVLMLTARTDEVDFVVGLDAGADDYVAKPF
                     RLAELMARIRALLRRGSPETLEASGVRLELTARRVLVDEQEVGLANKEFELLRVLMQHA
                     GQVVTREEILHEVWPEASRKGSKTLDMHISWLRRKLGDGNGRADEQRIATVRGVGFRFN
                     AD"
     misc_feature    20463..20477
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     gene            20604..21374
                     /gene="hisN"
                     /locus_tag="INP57_RS28225"
                     /old_locus_tag="INP57_28240"
     CDS             20604..21374
                     /EC_number="3.1.3.15"
                     /GO_function="GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA]"
                     /GO_process="GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hisN"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1207: inositol monophosphatase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315656.1"
                     /locus_tag="INP57_RS28225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28240"
                     /product="histidinol-phosphatase"
                     /protein_id="WP_374220986.1"
                     /transl_table=11
                     /translation="MAADLELALHLADTADARTTARFRAQDLDVTSKPDRTPVTDADLA
                     VEDAVRAVLAEQRPGDEVAGEERGGDASGPRAWVLDPIDGTKNFLRGVPAWATLIALVE
                     HGTPVVGVISAPALGRRWWAAVGQGAWASGGAGPERIAVSGVRELADAYVSTTHLGTWV
                     EHHSRESYLRLVDACWENRAFGDFWQHCLVAEGAIDLAAEPIVNPWDVAAAQVLVQEAG
                     GTFTDLDGTADHRGGTVLTSNGHLHADALALLRR"
     gene            complement(21394..21729)
                     /locus_tag="INP57_RS28230"
                     /old_locus_tag="INP57_28245"
     CDS             complement(21394..21729)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948685.1"
                     /locus_tag="INP57_RS28230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_28245"
                     /product="hypothetical protein"
                     /protein_id="WP_295141430.1"
                     /transl_table=11
                     /translation="MNTESVLGWLTAMGVPAELVSLGAEADNAWCLLRTEIDGVPGWEV
                     FWREQGNRYDWARFSSEQVACFYLFGRLTWTQALRGVIGPVDRTSKPTGTTVQEPATPP
                     SGTPAQS"
ORIGIN
        1 ggccgctcgc aggactaacc cggtgatctg ccgggtacgc aatcgacggg ggtcatcggc
       61 agcggtgcga cgccgctgcg gtggcccccg ttttcgcgtc ctgttcatcc tttgtggaag
      121 aattgagatc tttgaccaat ccgcccggcc accggctccg aaacactgat ggcaggtgcg
      181 catctgccag gcacgggagg tggcggatgg acgagtgtcc gatcgaccgg gaagtcagca
      241 ccgagcagct gctggtgaag atcggcagcg gagatctccg ggcgttccat accttctacg
      301 accgcacggt gcgaccggtg cggacgctgg ccagggaact gctcgacgac gacgcgcagg
      361 ccgaagaggt cgcgcaggaa gtgctcatcg aggtctggcg caccgcgccg cgctaccgcc
      421 cggaacgcgg cagcgcgcaa agctgggtga gcaccatgac gcgccggcgt tcggtggacc
      481 ggctgcgctc ggcgcgtgcg gtgcgggaac gcgacgaccg gttcgcggcg ggctcgcagg
      541 agaccgccta cgactcggtg accgagacgg tgctggcgca ctgcgagcac ctgaaggtcc
      601 gcagctgcct gtccgcgctg accgcgttgc agtccgaggc ggtgctgctc acctactacc
      661 ggggactgag ctatcgcgaa gcggccgagc agctgcgcat tccgccgtcg acggtgaaaa
      721 cgcggctgcg ggacggactg ctgcggttgc gcgaatgcct cggcgactgc tgcctggctt
      781 ctcccgggca agctcgagaa gatcgtccgt gacccgctga ccgacagttt tcccgcgttc
      841 ccgatcggtt cgtgggcgca aaccccgccg ctcgccgtcc gtcctgtgag gttccacacc
      901 ggaaaacctg gaagtgatcg acgggatctc gtgttgtacc ggctgcgaga tcgatcgacg
      961 gtctcgtcac ctaccggatt cgtcccgtac ctgaaagcgg aacggattcg aggaaacgag
     1021 ctagcggaag aggtgagttc tcatgcgcaa gctgaaccgt ggaatcgcag ccggactgat
     1081 ttcggccccc ctggtgctcg ggttcgccgg ggtcgcgggt gccgccgact tcgagggcac
     1141 ccagtcggtc gcgggtccgg aaggtgtgca gtccagccag accacggcgg gcgccgactc
     1201 caacggtgac ggccgggtct cctacgagga gagccactcg gtcgccggcc cggacggggc
     1261 ggcgacgcag cagaccagct cgagctcgga ctccggcagc ggtgactccg gctcggacgg
     1321 cggcctgctg ggcatcctcg ggctgtgaac ccgacggagc tgatcagctc cacctgcgcc
     1381 gtggccgcgg gaactcctcc cgcggccacg gccaccttga tgtccactgc ggacagtgga
     1441 aaaggacgcc gtcaacggcg cagcgcagca ccgtcggtct cgaaggcggc ggtgagcgcc
     1501 gatcgccagt ctcgcaacgg cgtcaaaccc tccgcggccc atgcgcgccc ggacagcacc
     1561 gagtgcgccg gtctgggcgc gggcagcgga aattcctccg tcgagcacgg ctgcacccgc
     1621 tccgggtcgg cgccgagttc ggcgaacacc gcgcgggcga aggagaacca gctggtccgc
     1681 ccggcgttgg tgcaatgcag caccgggcgc tgcgggtcgc ggcgctcggc gaacgatccg
     1741 gcgagctgca ccactcccgc cgccagatca ccggtccagg tcggcgcccc gacttgatcg
     1801 tccacaacgg acagcgagtc ccgctcccgt tccaggcggg acatcgtgcg cacgaagttc
     1861 tgcccgcctg cgccgtaaac ccacgcggtt cgcaccacgc aggcgcgggc tcccgcggac
     1921 agcacggccc gctccccatc cagcttcgtg cggccgtaaa cggtgcgcgg ctcggccgga
     1981 tcgtccggtt cgtacggcac atccgcatcg ccggagaaga cgtaatcggt ggacatgtgc
     2041 accagcggaa cccggcgggc cgcgcaggcc ctggccaacg cagcggctcc gtccacgttg
     2101 atcgaccgcg ccgcgtccgc atcgctctcg gccgcgtcca cggcggtgta ggcggcggcg
     2161 ttgaccacca ccgggcgcag atcgttgtcc agcgcggtct cggcgaacga gccgacagcg
     2221 tcgccgacct ccgcggcgtc gcgcaggtcc agttcgcccg atcccggcgc gtgcaccagc
     2281 ggcaccgccg aacgcgccag ctcccgtccg agctgaccac gcccgcccgg cacgagcagc
     2341 gcgaggcgct gcgcgctcac ttctgaacct gcttcaacgg ctcccaccac gaccggttct
     2401 cccggtacca gcggatggtc tcggccagtc cctcgtcgaa gccgacccgc ggccggtacc
     2461 cgagctcggc ggcgatcttg gtgtggtcca ccgagtagcg ccggtcgtgc cccttgcggt
     2521 cgtcgaccgg gcgcaccatc gagaagtcct gacccatcgc ctccagcagc cgctcggtca
     2581 gccgccggtt ggtcagctcg gtgccgccgc cgatgttgta gacctcgccc ggccgcccgg
     2641 actcggcgac cagctggatg ccccggcagt ggtcgtccac gtgcagccag tcgcgcacgt
     2701 tgccgccgtc gccgtagagc ggaacccggt ggccgtccag caggttcgtg atgaacagcg
     2761 gaagcagctt ctccgggaac tggtacgggc cgtagttgtt cgagcacctg gtcacgcaca
     2821 ccggcagctc gtgggtgcgg aagtaggccc ggctcatcag gtcggagccg gccttcgccg
     2881 ccgagtacgg cgaattcggc tccagcgcgt gctcctccgg ccacgaaccg gtctcgatcg
     2941 agccgtacac ctcgtcggtg gagacgtgca cgaacttgcc caccccggcc gcgagcgcgg
     3001 cctgcagcag cgtgtcggtg ccgaccacgt tggtccgcac gaagtccgat gcgcccagga
     3061 tcgaacggtc cacatgggac tcggcggcga agtgcaccac cacgtcgacc ccggtcatca
     3121 gctccgcgac cagctcggcg tcgcagatgt ccccgcgcac gaagcgcagc cgcggatttt
     3181 ccgccaccgg gcgcagattc tcctgattgc ccgcataggt gagtttgtcc agaaccacca
     3241 cttcggcgtc cgcgaacgcg ggataggccc cttgcaacag ctgccgcgcg tagtgcgaac
     3301 cgatgaagcc ggccccaccg gtgatcagta ctcgcatgcc accttccatg gggatttccc
     3361 ttccgccgca gggcggatcg gcagggaccg aacgtcgccc ggaagcagtc tgccacgctg
     3421 tcggccgggc gtggtcagcg ccgaccacgc agtgcgaaaa gcggcgtccg cgccgccgaa
     3481 caacacgcgg agtgcaagcg gcgaaccact cgcagtgatc ttccgactac gaagtacgtg
     3541 cggctgcggc gattccgccc gctcgcaacc ccgacggcgg aacaccgtta gcctgcgtgg
     3601 tggcttcgcc gaaaaatcac cccggcgagg tgtaacgctc gcaggaagtc ccaaggagga
     3661 ttcggcgtgg acgaccggtc gcgacggcca ggtggcccac cagggcaacc gggcaggccc
     3721 gtcccaccgc gcggaccgca gcgccagccc cgccccggtt ccggccccgc cggacgtccc
     3781 gcacccggcg gcggaccccg ccagccctcc caggaccgcc ccgcaccggc cgaccggccc
     3841 gcccgcgcgc agcagcgccc gccgggcgag cagcagcgac gacccgaacc gcggccggag
     3901 cggcccgctc cgccgccgcc cgaggacacc tggaaccgcg gccgcaacgc ccgccgcaac
     3961 ggcatgatcg tcgggcggac gttcgtggcc ctgctttccg tgctggtgct cgcattcacc
     4021 ggctacgcat gggcgaccct gcgaaatctg aacagcaacc tctccggcag cgacgtcatc
     4081 ggtggcggcc tgagcgaacc ggacggcgcc accgacatcc tgctgatcgg caacgacagc
     4141 cgcaccgacg ccgacggcaa cccgctgccg gacgagatcc tcaagacgct gcggaccacc
     4201 gacgacgaag gcggcgacct caccgacacg atgatcctga tccggatccc gaacggtggc
     4261 gggcgcgcca gcgccatgtc cttcccgcgc gacaccatgg tgcagatggg caacgggtac
     4321 ggggagaaca agctcaactc cgcgctgccc agggccaagt ccgacgcgaa gcagaagctg
     4381 caggccagcg gcgtcaccga cccgaagcag ctcgaactgc agtccaaggc cgaaggccag
     4441 aagttcctga tcaagaccct ggaagacctc tccggggtga gcatcgacca ctacgccgag
     4501 gtgaacctgc tcggcttcta cgagatcacc caggcggtcg gcggtgtcga cgtctgcctg
     4561 aaggacgagg tcaacgacag cgactactcc ggcgcggtgt tcaaggccgg gccgcagacc
     4621 gtcgccggtg cggacgcgct ggcgttcgtg cggcagcggc acgggttgcc gcgcggcgac
     4681 ctggaccggg tggtgcgcca gcaggtgttc atgtccgggc tggcgcgcaa gatcctctcc
     4741 agcgggacgc tgagcaaccc ggccaagctg accagcctga tggaggcgct gaagaagtcc
     4801 gtcgtgctgg acaagggctg ggacgtggtc gacttcgccc agcagatgca gggcatcgcg
     4861 ggcggcgaca tcagcttcca gacgatcccg atcgagctgg agggcgagtc cggttcggag
     4921 gacgtcaccg cgaacccgcg cgaggtcaag cagttcgcgg acgggctgct gctgccgccg
     4981 caggagcgcg cggccaagga gcaggccgag caggccgccg agaagcagcg cggcggcacc
     5041 accgtgaacg tcttcaacgc caccggcaag agcggcctgg cgggccaggt gctgagcacg
     5101 ctcagcgaag cgggcttcag ccagggcggc agctccaatt cggactcgat ggcgaagtcg
     5161 gtcgtctacc acgcaccggg cgacgaggcc gccgccggac aggtcgccgc caccctcggc
     5221 ggactgccgc tgcaaccgag cggcaacctg cagtccggca acgtcgaggt gtacgtgggc
     5281 agcgactacc aaggccccgg ggcgcagcag ttcgccggga acaagcaggt ccagctcgac
     5341 ggcgggcaac gcgccgcggc accgctgcac gcccagcagt ccggttccgg cgacgcgccg
     5401 atcgacgcgg gcggcgtccc ctgcgtgaac tgaccgggtt ccgcgccgcg ttaagctgat
     5461 cgcgcgcgtc ggccggaagg tggacccgcg cgggcggacc gccggtccgg tcccggtggc
     5521 aggccgccag ggggagggaa cgtggccgag gaagacgtgc cgaccgcggg ctcgggcgac
     5581 tcgcgggaac cggacgagca gcgggccgcg caacccgacc acgggcgcgc tgcggacgcg
     5641 gcgggtggcg gctcggaccc gggcgacccg gcttcgcacg gcactgcatc ggggcgcacc
     5701 gcgtggcacg gcacggtttg gaccggcacc gtctggcccg gcgacgcatc gggcggcacc
     5761 gcatcgcgca gcgctgcttc ggatgacacg gcatcgcgca gcgccgcatc ggacgacacg
     5821 acatcgcaaa gcgccgcatc ggccagcgcc gcgtcgcgca gcaccggatc ggacggcgcg
     5881 gcggcggccg acagctcagt tctggacggc tcggaaccgg acgacgcggg cggatccgag
     5941 cgcgccgctg ccgccccgcg caagcaccgc ggctggctga tcgcgggccg gacgctggtc
     6001 gcgctgatct ccgccggagt cctggcggtc accgctgcgg gttgggtcgc gctggaccgc
     6061 ctggacaccg acgtcagcac ctcccgcgta ctcgccgaca cgccggaagc tcccccggcc
     6121 gacgacggcg cgaccgacat cctgctggtc ggcaacgaca gccggaccga cgcgcaaggc
     6181 aacccgctgc cgctgagcgt gctgcgcgaa ctgcgcacgg agagcaccaa cggcctcaac
     6241 accgacaccg tgatcctgat gcggatcccg cacgacggcg gcccgcccac ggcggtttcg
     6301 ctgccgcggg acacctcggt gccgatgccc ggcggcggca cccagaagat caatgcggtg
     6361 tacggcctgg cgaagagcgc cgccgcggat tccatcgaag gccgcgactc ggcgctggcc
     6421 gagcggcaag cccagctggc cgggcagcgc gcgctgctgc agagcgtgca gcggctcagc
     6481 ggcgtccgcg tggaccacta cgccgaggtg aacctgctcg gcttctacga ggtgaccgac
     6541 gcgctcggcg gcgtcgaggt gtgcctgaac caagccacca gcgacaagga ctccggcgcc
     6601 gacttcgccg cgggcaggca gaccatctcc ggcggcgacg cgctgtcgtt cgtgcggcag
     6661 cggcacggcc tgccgggcgg tgatctggac cggatcgtgc gccagcaggt cttcatggcg
     6721 gcggtggcca acaagctgct gtccaccggc acgctgaccg atccggcgaa gctggccggg
     6781 ttgcaggacg cggcgcggcg ttcggtggtg ctggacgaga actgggacgt ggccgggttc
     6841 gccgcgcaga tgcagggctt ggcgggcggc ggcgtgcggt tcgtgacgct gccggtcgct
     6901 tccaccggcg gcagggacga gcacgggcag agcgtggtca aggtggatcc cgcgcaggtg
     6961 cgttcgttca tcgcgggcct ggtgccgaag accggtttcg ccgcgaacgc cccgctggac
     7021 ctggacggca cggcccggcg ccagcctgcg ccacccgccc aggacccgat cacctccgac
     7081 ggcatcccct gcatcaacta gccgcagccg atccgaggca ggggttcctt cgccccaaca
     7141 gaccgagcga tcgagtcgct cacccccgac gtcagctccc gcgcggtccg atcgaggggc
     7201 tccttcgcgc caataggccg gtcgaacggg tccttcattc ctcgcggtac gcgcggtgac
     7261 gagcgccttc gcgccggtgg gacgctcggt ccccttcgcc gcgggcggcg ggctacgcca
     7321 taaggtcgcg gcatgagcgt cacgcaagca ttgctcgcac ccatgctggc aaccggttcc
     7381 ccgcgcccgc tgatcacgca ctacgacgac gccaccggag cccgtgtcga gctgtcccgc
     7441 gcgacggtcg cgaactgggc ggcgaagacg gcgaactggc tggtggacga gctcgacctg
     7501 gagccgggca cgccggtttc ggtggcgctg cccgcgcact ggcagaccct cggagtgctg
     7561 ctcggctcct ggtggtgcgg tgcgcacgtg gtcgacgacc cggcgggtgc cgaggtcgcg
     7621 ttcgtcccgg ccgccgcgcc cgagtccggc gcgggcgcgc acgtgaccgc cgtggtgggc
     7681 ctggacgcgc tcggcggccc ggtgcgcgag ctgccgcaag gagccgtgga ctacgtcagc
     7741 gacatccggg tgcacggcga cgagttcagc ccgcgccaac aggttcccgg cagcgctccg
     7801 gcgctcgtcg ggtccacagt ggacgaagtg ctgggcgtgg ccgcggagcg ggccgaagcc
     7861 ctgggcatcg gcccggactc ccgcgtgctg tccacattgg agtggacgtt gcccggcggg
     7921 gtctacgacg ggctgctcgc cgtgctgtcc gcgcggagtt cgctggtgca ggtcagcaac
     7981 ccggacccgg acaagctcgc gcagcggcgc accaccgaga agaccactgt ggacctcggc
     8041 gcggtgctgt gaacaccggc gggccggtcg accaccggcc cgccgcagca ctccgaactc
     8101 cttcagacgc agcgacccgc cctgccgccc agcgacttgg ccgcgctcag acagctgcgg
     8161 gcgcggagtc cttccgcgcg gcgaaggccg cctgcagggt gtatttcggg ccgctgaagc
     8221 cgagcgcgag gctcaccgcc gccagcacca gcacgtactc gaagccgcca tcggtggcga
     8281 agtacgcccc ggagtgcgcg gtgaccagcg cgccgagcat cacggcagcc agcagcacac
     8341 cggcgaccgg cagcagcacg ccgaggatca gcgcgagccc gccgagcagc tcgaccagcg
     8401 cggtgaacca ggccgcggca gcgggcagcg ggatgcccat ctgctcgaac ccggcgctga
     8461 cgccggacat cccgttgatc gccagcttct ggtaggcgtg cagcacgaag gtcaccccga
     8521 cgaccagcct ggccagcagg gtcgtcaggt cgcggtaccg gtgcatcggc tttcctcccg
     8581 ggtcggtggg cgccgccagc taagcatgaa agttcaacta aatcgctccc gcgcggaatc
     8641 ggatcacacg gacatcggcg aaattccgcc gaaggttcgc agcggcacga ccgaacgcag
     8701 cacgcctacc caaagtaagc taccggcaag tagcattcgg gcgtgcaaga cgagaccctc
     8761 ttccggcccg cgggcgagct gttccttccc accgactcgg cgggcaatcc gtggggcgaa
     8821 ctgaccggcg gtggccccgt cgcaggcttg atcgcccgcg ccgtgcagcg cgaactgtcc
     8881 gatccggacc tgttcctcgc acgtctcacc gtcgatctgc accgcccggt tccgcggcgc
     8941 accggcgtcg ccgtgcacac cagaacggtg cgcgagggca agcggctgcg cgtgctggaa
     9001 gtggtgctgc accaggacgg gaccgaggtg acgcgggcac tcgcgcacgt gctgcgccgc
     9061 gaccggacgg acgaagccga acccgatggc gagatcccgt tcgccggacc ggacggaatc
     9121 accgacggca ccctgctgcc cgccgggctc ggcctgcgct ggggcgtgca cgacgtgatc
     9181 caggtgcgct ggctgtccga ccagatctcc ggcagcccga gcagggcgtg gatgcgcctt
     9241 ccgctgccgc tgctgccgga cgagccgacg agcccgctca cccagctcgc ggtgctggtg
     9301 gactgcatca gcgcggccag tccggtcggc gccctgttcg ggccgtggat caacagcgac
     9361 atcacgctgt acctgcaccg cgagccgcgc ggcgaatgga tcggcatgga gatcgagcgc
     9421 gacgtcgagc cgaccggcat cggcgtggcc cgtgcgcggc tgttcgacga gcacgggccg
     9481 atcggcacgg cgcacgaggc ggtgctggag caccagctgg gttgatgcgc gattcgcaca
     9541 ctctcgcgca tttcgcgtgc acgatttccg gaacgctcgt tacacgccta gtaccacctt
     9601 caccgattcg ggtcatcctg cggaaaaaag attgtgcccg ccgcacggcg acgcgcactg
     9661 tgatcgtcgg cagagatcag ccgaacgccc ttcgatagcg cccgcgagac ccgactctcg
     9721 gcgcaccttg caggcccccg ggcgcgtatc cggcgcgcgg ggcggcgttc gcaggcaccg
     9781 ggatcccggc ggtcttgcgg accaccggcg atcgccggct gatccgctgc cgggtagtcg
     9841 ccgagaacgt cgtgaagagg aggccgtcgt ggaggagcaa gccgcaccgg aaccctacga
     9901 accgccggta ctggtggaag tcggagaatt cggcgcggac gccgccgcgg acggttccgg
     9961 gcaattcgat gcctccgttc gctgatcggc ggaaaatgcg atgaacacgc agcgccgaga
    10021 ttccgggtgg gtggccctgc ccgatgcacc ggagatgcgg gcggtggccg accggttcga
    10081 tccgcacggc cggatcgtgc tgccgcacgc ctccgggaac ccgtggctgg tcggccactt
    10141 cgccccggac gaactcgttc cggtggcgct ggggccggtg cgggtggcgc tggtcggacg
    10201 ctgcccgatc gacgcggccc ggctggccga gctggcccgc gcggtgcact cggtgtccga
    10261 tgtggacggc atcgtccggg cgctgcccgg cggggtgcac gtggtggtct cggtgggcgg
    10321 cgacgtccgg gtgcagggcg ggatttccgg gctgcgccag gttttccacg gcgtgcgcga
    10381 gggcttcccg atcgcaggcg accgggccga cctgctcgcc gggctgctcg gcgcgggcat
    10441 cgacgaccgg gcgctcgcgg cgcaggtgag ctgcgcgcaa ctgcccccgc cgctggccgg
    10501 gcagagcatc tggtccggca tccgcgcggt tcccgcggac agctacctgc ggctgctcgg
    10561 gcaccgggcc accgaggtgc gctggtggca cccgccggag ccggaggtgc cgctgcgccg
    10621 gggcgcgcgg ttcctgctgg aacgactcga atccgcgatg gaaaccggcg cgggcgaggc
    10681 cgggcgcgcc ggatcggagc tgtccggcgg aatggagtcg gccgcgttgt gctcgctcgc
    10741 ggccgcgcgg aattcggatc tgctcgcgct gcggatggga aacggcggac gcgaatccgc
    10801 ggcgccggaa tggcgatcgg ccgaaccgct cgtgctggac gacgcggaac tcccggatat
    10861 gttcgccgaa ccggacgcag cggccgatcc cgaacatcct tatccgggct ggcgcaccgc
    10921 cgcccgcatc cggtattccg cacgaattct cggcgaacgc ggagtgcgca ggcatttcgc
    10981 cgcgcacggc ggggaagaac tgttccacga cggtcccgca catctgcacg cactgctgcg
    11041 gcaccggccg gtcaccgcgc tgcggcggct gcgcggctac cgcgcggcgc ggaattgggc
    11101 gctgccaccg acgatttccg cgctgctgag cgcgcacgac gtcggacgct ggtggcacga
    11161 acaggcggat cggctcaccg agccctatcc cgactcgaac gccccgcgac tggactgggg
    11221 agccgccccg gtacgcgtgg cgccgtgggc gagcgcggaa gcggtgagca ccacgcgtgc
    11281 gctgctgcac cggaccgcgc gcaagaccga gcggctggca ccggaccggg cgacgcatca
    11341 gctgctgtcc gcgctgcgca ccggcgcgcc cgcgcaccgc aggctcgccc gcagctacgc
    11401 ggcggcgggc gtgcggctgg agctgccgtt ccacgacgac cgggtggtgg aagcggtgct
    11461 ggcggtgcgc ccgcacgaac gctgctcccc gtgggaggac aagccgctgc tggccgaggc
    11521 gatgcgcgag gtgctgcccg gtgccctgcc gcgcagggcg aacaccggcg acggcgaggc
    11581 gatccgcatc ggtttccggc gcaaccgggc cgcgatcctg gcgttgttcg ccgattccga
    11641 actcgcccag cacgatctga tcgacaccgg cgtgctgcgc aggcacctgc tggccccgca
    11701 cgtcggcgag cacacgctgt tccagctcga ccacctgctg ggctgtgaga actggctgcg
    11761 cagcgccacc cgagccggga gaacggatgt ccctgcgact gcgtcctgac gtggccacca
    11821 ccgacaccgc cgagggaacc gtgctgctcg acgagcgcac cggccggtcc ttccagctca
    11881 accgaaccgg cacgcacgtg ctgcggggtc tgctgctggg gcaggacacc gaacacatcg
    11941 cccaggaact cgccgagcgg caccggatcc cgccgcaccg cgcgaaacga gacgtggcca
    12001 ccatcgtgga acgcctgcgc agcgtcaccc tgctggagtc ggcatgagcc tgcccgtcac
    12061 cctgcccgtc cgggagaacc tgccgctgcg ccaacgcgct tcggccttgc tggccgtcgg
    12121 gctggcgcgg ttactcgtgc tgctgcgacc cgcgcgacag ctcaagctgc tgcgcttcgc
    12181 ccgccgccgg gccgtgcccg cgagtgccac gcaggcgctg gcggccaggc gcgacgtcct
    12241 cgcggtgagt ccgcgcagcg ccgactgccc gctgcggcgg tccatcgcca cggcgctgct
    12301 gtgccgcgcg cgcggagtgt ggcccacctg gtgcacgggc gtgcgcaccg cgccgttcgc
    12361 ggcgcacgcc tggatcgagg tcgacggcca ccccgtcggc gaaccgcacg cgtcggcgca
    12421 ttaccgccgc ctgcaaagca tcccgccgcg ctgagcgctc agcgcctcgc ggttcactgc
    12481 tcggtggcgc ggctctgcag ggcggcgtgc ttgtcgtgga ccatcgattc caagtcggac
    12541 tggaaccggg ccatcgcggt ccgcaagcgc gtgccgagct ccccggaatc cgcggcgagc
    12601 gtgcggaccg cgagcagccc cgcattgcgc gcaccgccga ccgagaccgt cgcgaccggc
    12661 acgcccgcgg gcatctgcac gatcgacagc agcgagtcca tcccgtccag gtgcttcaac
    12721 ggcaccggca cgccgatcac cggcagcacc gtcgccgcgg ccaccatgcc cggcaggtgc
    12781 gccgctcccc cggccccggc gatgatcgcc cgcaggccgc ggtcggccgc ttcgcgggcg
    12841 tagtccagca tccgctgcgg ggtgcggtgc gcggagtaca cgccgacctc gtagggcacg
    12901 tcgaactcgc tgagcgcttc cccggcggcc tgcagcaccg gccagtccga gtcgctgccc
    12961 atgatcacgc cgaccagcgg ctgcaccatc ccgatccctt ccacacgcac accaacgacg
    13021 tcgagtgaac ggactgttcg ccccaataga ttggtcgaac ggtccgttcg ctcaaaacct
    13081 tccgcccggc agggccgctc agtgcacgag gtggccgtcg gcccattccc cgtgcgacag
    13141 gaagtgcgcg gccaggtcgg cctcgcggcg caccgcgctc atgtcctcgc cgagcacgtt
    13201 cacgtgcccg atcttgcgcc ctgggcgctc ggccttgccg tagaggtgca ccttcgcgtg
    13261 cgggaatcgc gcgaacaggt ggtgcatccg ctcgtcgacg cccatcttcg gttcggtggg
    13321 tgcgcccagc acgttcgcca tgaccacggc gggcgcggtc aggcccgtgc tgcccagcgg
    13381 gtagtccagc accgcccgca cgtgctgctc gaactgcgaa gtgagcgcgc cctccatgct
    13441 ccagtggccc gagttgtgcg gccgcatcgc cagctcgttg atcaccaggc cgtcggcggt
    13501 ctcgaacagc tccaccgcca gcacgccgac cacgccgagc tcttcggcga tctgcagcgc
    13561 catgttctgc gcctgctcgc gcagctgctc cgaggcgtcc ggggcgggcg cgaggacctg
    13621 cacgcagatc ccctccgact gcaccgtctc gaccagcggc cacgcggcgc cttgtccgaa
    13681 cggggaacgc gcgaccagcg cggcgagctc gcggcgcagc ggaacccgct gctcggccag
    13741 cagcacggcg ccggcttcga gcagctcggt gacggtgcgc tgcgcgctct ccggcgtgtt
    13801 cagcgtccac acgccgcggc cgtcgtagcc gccgcgagcg gtcttgagca cgcagggcca
    13861 gccgtgctcg gcgccgaact ccaacgcgtc ggagaccgag cggatctccg cgaacggcgg
    13921 caccggcagg cccatctccg cgagcttgcg gcgcatcacc agcttgtcct gcgcgtgcag
    13981 cagcgcgtcc gggccggggt gcacggcggc acccgcggcg accagctccc gcaggtgctc
    14041 cccgggcacg tgctcgtggt cgaacgtgac ggcgtcgcag ccttgcgcga acgcgcgcag
    14101 cgcggccagg tcggtgtggg gaccgatctg gacgtccggc gcgaccagcg ccgccgggtc
    14161 gtccggggcg gcggccagca ccttcaacga ctgccccagg gcgatcgcgg cctggtgcgt
    14221 catccgcgcg agctgtccgc caccgaccat gccgacgacg ggagtacgag tgcggtggtc
    14281 cacggctacg cagcttaaag acgcccgcac tgatcatctc gatggggacc gccgaatggc
    14341 gacgtgcggc actttcgcag cggccgagcg gcttgctcga actcagtcca gcgtgccgag
    14401 catcgcgcag gccgtggcgt ggtcctccgg cagtggctcc tcctgcaccc gcaccggccc
    14461 gaccggccgc agcgccggat cggccgcgat ccgctgtgcg gcctgcagcc gaacctgctc
    14521 gcagcgctcg gagaaccgct cgtcccgcgg cgccagcacc gcagccgtgc tcgtgcgcag
    14581 caacgacacc gtctcaccgg agtcgaacac ctcccgcagc acgtcggcgg cgagcaccat
    14641 ggccatcatc agcggatagc ggcccgaagc ccgcggcatc tgcacggaca cgctgatcag
    14701 cacgtggtcg gcgtccgggg accgcccggc ggcgcgcgct ccccggtaca cctcgtgcag
    14761 ccgggtccgc aggtaggacg cggtggtcag gccggtcagc gggtcctgca cctcgcggtc
    14821 cacggtgtgc tcggcctgca catcggccca gcccagcgcg accggccgca gcatcgctgc
    14881 gggcaccgcg tccggatcgg ccgagaccag cccgtcctgg tgcgagcccg tgctcaccgc
    14941 gtgcagcgcg gcgacgtcct gcaacgcctc ggcgagcccg acaccggatt ccgcgcggcc
    15001 cgcgccgagt tcgcccagcg gctccgccag ctccgcgccg gtcgccaccg cgcggcacac
    15061 ctcgcgcacc gcccggatgc cccagtcgct cgggaagggc catcccgcgg cgaggctcgc
    15121 ggtgcgccag cgcgcccgca gcgcccgctc gccgcctgca gcgctccgga tccgttcagt
    15181 catctccgac ccccttcggc aactgggacg acctgcgggt tccggtatga cgcaagactg
    15241 cctgcggcgc ctgcggagga gtcgatcaca atggtgggcg gtcggttcgc accgcgttgg
    15301 cggatcgacc tccggcaccg cgaatggttc gattgggcgg ttcttgcgct gatcggcggt
    15361 gccggtgcgc ggccgttcgc cgcagcgccg accggcgtct cgccgaaggg gcgccgcggc
    15421 ggatcgagac agcggaccac gaaggaaggt gcggcgtggc cacagccccg gcagaagcgc
    15481 aggaccccag cgacggggaa ctgatcgaag ccgtccggca cggttccacc gaggcgtacg
    15541 gaacgctgta cgaacggcac gtggccgccg cgcacaacat ggcccggcag gtcgcccgct
    15601 ccggatcgga ggccgacgac ctggtctcgg aggcgttcgc gaaggtgctc gacgcgctgc
    15661 gcggcgggca cggcccgagc accgcgttcc gcgcctacct gctgacctcg ctgcggcacg
    15721 tggcctacga ccgcacccgc cgggaacgca agctgcagct ggccgacgac gtcgcggagg
    15781 tctccggggc ggatctgagc gtgccgttca acgacaccgc cgtggccggg ctggagcgtt
    15841 cgctggccgc gcgggcgttc acgcagctgc cggagcggtg gcagaccgtg ctctggcaca
    15901 tcgagatcga agggcagacc cccgcgcagg tcgggccgat cctgggactc acgccgaacg
    15961 gcgtctcggc gcttgcctac cgcgcccggg aagggctgcg gcaggcgtac ctgcaggtgc
    16021 acctgagttc gctggatccc gagcagccgg gcctgcaagc ctgccgcgcg gcggtcggca
    16081 ggctcggcgc gtggaccagg ggcgggctgt cccggcggga aaccgcgcag gtcgatcacc
    16141 acttggacga ctgcgaccgc tgccgcgcgc tcgcggccga gctggccgag gtcaacggcg
    16201 ggctgcgcgc cgtggtggcg ccgctggtgc tgggcgccgg tgcggccgcc tacctggcgg
    16261 cggcaccggc cgcgggatcg gtctcggctg ccggggccgg tgcggcgggt gcggctaacg
    16321 cggcgagttc ggtgccgcgg caggcgatct ccagcggggc cgccgcgacc gcgctggtgc
    16381 tgaccgtgct gctggcgctg acctccggga ctgcctcgct ggggccggtc gcttccgacc
    16441 cgcccgctcc gcaggcggtg ctgccgcagc caccggcgcc gaaacccgcg ccaccgcagc
    16501 cgcaacgggc tccgcagccg cagccggagg caccggcgcc gcccccggcg atcccggatc
    16561 tgcacgcgac ggggcccgcg cagccgttgg agctggtgcc cggcgggccc gcggtcgagc
    16621 tgccgatcac cgtgaccaac cgcggtcaga tgacttccga gccggtcacc gcgacgctct
    16681 cgttgccgcc cggagtgagc gccgaggttc cgggggcgac cacgcaggcc agcggcccgg
    16741 caggcaccgc tccggcgagc agcaccgccc ccgccagcac tacgcccgcc agcactacgc
    16801 ctgcgagcgc tgcgccggcc ggcaccgctg cgtccagcgc gggtccgcga agcactgctc
    16861 cagccggctc cggctcggca ggcattgcca ccgcacgcgc cgctccagca agcaccggac
    16921 ccgcgagcac cactccggcg agcgcccgct ccgccgcacc ggccggcgag ccgcaggttc
    16981 gttgccaggc gtacgagggc ggcgtgcggt gcacttcgga tcgcggtgtg ccgccgggtg
    17041 gccacctcac cttccgcctg cgcgtgcagg cggcgccgaa cgcttcggaa ggcgagatcc
    17101 gcgcgcagat cacctccggc gccacgatcg acctgcagct ctccgcgatc cgggtccggc
    17161 tggcccccgc cgacggcgtg gacgtgcggg tctcccgcgc ggcgttcgac gagggcctgt
    17221 ccgagcaggt cgacgatcat ccgccggtgc acctcgaact ggacgtgcgc aacaccggcc
    17281 gcacggccgg tcgtgccgaa gcgatggctc ggctgccgga aggcacccgc gcgatcggga
    17341 ttccgccgga gtgcgaggtg cacccgtccg agcggcagct gcggtgcgcc aaggacttgc
    17401 ggcccggcga gtccttcgag ggcgacctgt ggctgctgac gttccaggac gcgcccgtgg
    17461 cggacgccga gcggcaggtc accattcccg ctcaggccac gctcgggcag gcgagcgaca
    17521 gcgactcgct gaccttgcgg ttgcggtatc cgggagtgcc accgaagccg tgcccgggca
    17581 ttccggagtt ccccggacag ggctggtggc actgctggtg gcccgagccg cccgagtccc
    17641 cagaacctcc ggaaccgccc gagccaccgg agccgccaca gcctccggaa ccgccccggc
    17701 cgccggaatc accgcagcac ccggaaccgc ccgaaccacc ggaactgccg gacctgctgc
    17761 aaccgccgga cgacccgccg cagcaggaaa cctcgcggcc ggaacacgac gagccggacg
    17821 cgccgtccgg aacccggccg tggccctggc cgctgcggga cctgtccggg cggtgactca
    17881 ctgccgcgtg cggcggtgac tcactgccgc gtgcggcggt gactcactgc cgcgcacgcg
    17941 gcggtccgcg ccgacgaccg gccaccggaa gcgcgccgac cgccacgtaa aattgccgcc
    18001 gtgtccgtcg tcgattcggt gctcacccgc atcccgcagc cgtaccgaaa cgtggcgatc
    18061 cggcaccgcg aactgctcaa gttcgcggtc gtcggtgcga tcacgttctt cgtcgacacc
    18121 gcgatcttct acgggctgaa gctgacgatc ctggtgccga aaccggtcac cgcgaaagtg
    18181 atcgccgtgc tggtggccac gatcgcctcc tacgtgctca accgggaatg gtcgttccgc
    18241 accaggggcg ggcgcgaacg ccaccacgag gcggcgctgt acttcctgtt cagcggtatc
    18301 ggggtcgcgc tgtacgccgc gccgctgtgg ttctcgcggt acgcgctgca cctgcaggag
    18361 ccctacacca gcaggttcgt cgaggagatc gcggacttca ccgccgcgca gatcgtcgga
    18421 ttgctgctgg gcatggcatt ccggtggtgg gcgttccggc gctgggtgtt cccttccggc
    18481 gattccgaac aagacgaata gctcgccgcg attcgtctcg gccgcacgaa accgggtggc
    18541 ctcagcgcgg cgtcgactcg atcttccagg gaacgccgag cacgtcatcg gcacgtgcca
    18601 ccggcaggta gatctcgaac agcgcgggcc gggccttgct gagttcgagc cgcccgccgt
    18661 ccgcctcgat caacgcccgc gccagcgcca gcccgacacc ggtcgagccg cccgcggaga
    18721 acccgcgatc gaacacgtac ggcaccagct ggtccggaac gcccggcccg gcgtcgccga
    18781 cctccacgac gaccgtcccg ccgccctcgc gggcagccag cgtcaccgct cccttgccgt
    18841 gccgcagggc gttgtccagc agcaccccga tggcttcgcg caaccgggcg ggcgtcgccc
    18901 tggccagcag cccctccggc acccgttgcc gcagcgtccg gctctgcgcg cgcagcaggt
    18961 cccgccactc cgtcgccgcc tccgccagca acgccgagac gtccagcggc tccgcgtccc
    19021 gcgccctggc cgcggtggcg gcggcgagca ggtcgtcgag cacgctggag agccgctcgg
    19081 cctgctccag cgcggcggtg gcctcctccg agacctcctg gtccggcact tcggccagcg
    19141 cttccagacg cagctgcaag gcggtgagcc tgctgcgcaa ctggtgcgac acgtcgccga
    19201 cgagttcccg ctcccgctgc accaggtgcg acaacgccgc gccggaagcg tccagcgcgt
    19261 cggccacccg gtccagctcg ggcacctcgt gccgcttcgg gtcggcccgg aagtcgcccg
    19321 cgccgagccg cgcggcccgc gccgcgacgt gccgcagcgg gtcggcgagc cgccgcgcgg
    19381 tcagcccggc gaccaccatc ccggtacccg ccgagatcac caccagcagc agcaccagcc
    19441 ctgcgacctg gaactgctgc gtgcgcaccg gcgcgctcgg agccgacagc gtgacggtgc
    19501 cgctctgcac catcggcacc ctctcggtca gcggcgaggg ccccgggtcg gagccgtaca
    19561 cgtagtcctg ctgcttggtg cgcaccacca gctgcgcccc gcgcggcacc gccagcagca
    19621 ccgactcgac gtcgatcggc cggtgcgaag cgacctgctc gtccagcgag gtcgcgatct
    19681 gctgcgagcg ggtggacaga tcggcccggg tgaagtcctc gaccagcttc agcgcggtca
    19741 cgcccagcgg cagcccgagc accacggccg tgaccgccac cgccagcaac gtcgccgcca
    19801 ggatccgcct gcgcatggtc agtccgcgtt gaaccggaac ccgacgccgc gcacggtcgc
    19861 gatccgctgc tcatcggcgc ggccgttgcc gtcgccgagc ttccggcgca gccacgagat
    19921 gtgcatgtcc agggttttgc tgcccttgcg gctggcttcc ggccacacct cgtgcaggat
    19981 ctcctcgcgg gtgacgacct ggcccgcgtg ctgcatcagc acccgcagca gctcgaactc
    20041 cttgttggcc aggccgacct cctgctcgtc gaccagcacc cgccgcgcgg tgagctccag
    20101 gcggaccccg gacgcctcca gcgtttccgg cgagccgcgg cgcagcagcg cccggatgcg
    20161 ggccatcagc tcggccagcc ggaacggttt ggccacgtag tcgtccgcgc ccgcgtccag
    20221 gccgaccacg aagtcgacct cgtcggtgcg ggcggtgagc atcagcaccg ggatgccgcg
    20281 gccctgcgcg cgcagcctgc ggcacacctc caggccgtcc atttccggca gcccgaggtc
    20341 cagcaccatc aggtcgatca cgccggacga cgcctcccgc aacgcggcag ggccgtcctc
    20401 gatcacacgc accgagtagc cctcccgctg cagcgccctc gacaacggca cagcaatggc
    20461 tgggtcgtcc tcggccagca gcactacgct cacgctcccc accctagatg tccgccaaga
    20521 tcagactcgt ctctagggag cgttcgccgg ggggcggggc cgctgcgcca tcggcgggca
    20581 cgattaaggt tccgagcgtg acgatcgccg ctgacctgga actcgcgctg cacctggccg
    20641 acaccgcgga cgcgcgcacc accgcccggt tccgcgccca ggacctggac gtgaccagca
    20701 aacccgaccg caccccggtc accgacgcgg acctcgcggt ggaggacgcg gtgcgcgcgg
    20761 tgctggccga gcagcggccc ggtgacgagg tcgcgggcga ggagcgcggc ggggacgcct
    20821 cggggccgcg cgcctgggtg ctggacccga tcgacggcac gaagaacttc ctgcgcggcg
    20881 tgcccgcctg ggcgacgctg atcgcactgg tcgagcacgg cacgccggtg gtgggcgtga
    20941 tcagcgcgcc ggcgctgggc aggcgctggt gggccgcggt cgggcagggc gcgtgggctt
    21001 ccggcggcgc gggccccgag cggatcgcgg tgtcgggcgt gcgggaactc gccgacgcct
    21061 acgtgtccac cacgcacctg ggcacctggg tcgagcacca ctcgcgggag tcctacctgc
    21121 ggctggtgga cgcctgctgg gagaaccggg cgttcggcga cttctggcag cactgcctgg
    21181 tcgccgaagg cgccatcgac ctggccgcgg aaccgatcgt gaacccgtgg gacgtggccg
    21241 ccgcgcaggt gctcgtgcaa gaggccggcg gcaccttcac cgacctggac gggaccgcgg
    21301 atcaccgcgg cggcacggtg ctcacctcga acggccacct gcacgcggac gcgctggccc
    21361 tcctgcggcg ctgatcgctt caggactggc gcttcaggac tgggcgggcg taccgctcgg
    21421 cggcgtggcg ggctcctgca cggtcgtgcc ggtgggcttc gaggtccggt cgaccgggcc
    21481 gatcacgccg cgcagcgcct gcgtccaggt gagcctgccg aacaggtaga agcaggcgac
    21541 ctgctcgctg ctgaaccgcg cccagtcgta gcggttgccc tgctcgcgcc agaacacctc
    21601 ccagccgggc acgccgtcga tctcggtgcg cagcaggcac caggcgttgt ccgcttcggc
    21661 accgagcgag accagttccg cgggcacgcc catcgccgtg agccagccga gcaccgattc
    21721 ggtgttcact ccgcgctccc ctcagtcgct tcctcccgcg cggcagccgg ctcagcgcgc
    21781 atccgagccg tgcccgcctc cgtctcggcc cgctgcacgg tcgaacgttc cagccggatg
    21841 gtgccggttt cctccaacgc cggggcgagc tccgcgagca ggcccaacgc caccagctcc
    21901 gccaacgggt gcgtggtccg gtagcggacg ccgccgccgc gcgcgccgaa ccacggcgcg
    21961 gtcacggctc gccacaccgg caacgggtgc agcacgcggt accggtggta cggcagctcc
    22021 aggtactcgg gcggcaagct gcgctcggtg aacgccgtcc cctccggcgc agcgacgcgg
    22081 ccttcgccgt cgccgagctc gtcgaggacc gtgtccgggt cgagcacgat cggctcaccg
    22141 gcctcggtgc agccctccgg gaactcgtcg ggcggccacc ggtaatcgga tccggtgctg
    22201 cggtattcgc ggtcccacgc gtgctcggac agttcggcca gcggctcgtg atcggcggtg
    22261 aagtcttcgg ggcgcgacgc agctccgcgg gcatccggcg ggggaagttt cccggagcgc
    22321 acccgggcca acgcgtcccg gtcaccgacg agctccgagc gcgggtgatc cagcggcccg
    22381 aagcggcgcg cggcccccgg atcgggcaac tgcctgctgg cgttggtctg ggcgaccggc
    22441 agatggccga tcgg
//
