LOCUS       NZ_JADDUE010000013     22783 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Saccharopolyspora sp. HNM0986
            NODE_13_length_147214_cov_67.050689_cov_67.050689, whole genome
            shotgun sequence.
ACCESSION   NZ_JADDUE010000013
VERSION     NZ_JADDUE010000013.1
KEYWORDS    WGS; RefSeq.
SOURCE      Saccharopolyspora sp. HNM0986
  ORGANISM  Saccharopolyspora sp. HNM0986
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Saccharopolyspora.
REFERENCE   1  (bases 1 to 147214)
  AUTHORS   Li,F.
  TITLE     Diversity and distribution of actinomycetes associated with coral in
            the coast of Hainan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 147214)
  AUTHORS   Li,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2020) chool of Life and Pharmaceutical Sciences,
            Hainan University, 58 Renmin Avenue, Meilan District, Haikou, Hainan
            570208, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016526145.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 02:15:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,899
            CDSs (total)                      :: 5,838
            Genes (coding)                    :: 5,784
            CDSs (with protein)               :: 5,784
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 7 of 54
            Pseudo Genes (incomplete)         :: 49 of 54
            Pseudo Genes (internal stop)      :: 0 of 54
            Pseudo Genes (multiple problems)  :: 2 of 54
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:09:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 19374
            Orig. end                         :: 42157
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADDUE010000013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22783
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="1"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22783
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22783
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12783](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12783
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     misc_feature    complement(207..221)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.98"
                     /tool="antismash"
     misc_feature    complement(208..223)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 20.07"
                     /tool="antismash"
     misc_feature    211..226
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 24.88"
                     /tool="antismash"
     misc_feature    211..229
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.44"
                     /tool="antismash"
     misc_feature    213..227
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.44"
                     /tool="antismash"
     misc_feature    complement(214..229)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     misc_feature    complement(313..327)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 22.41"
                     /tool="antismash"
     misc_feature    314..328
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 23.2"
                     /tool="antismash"
     gene            1048..1218
                     /locus_tag="INP57_RS25055"
                     /old_locus_tag="INP57_25065"
     CDS             1048..1218
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="INP57_RS25055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="INP57_25065"
                     /product="hypothetical protein"
                     /protein_id="WP_200072804.1"
                     /transl_table=11
                     /translation="MDRYVVEPNFPDAGGLSEDGLRTLSAANEVLRSTHYDLQLLVRVT
                     SPDEPSQTASA"
     misc_feature    complement(1064..1078)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.93"
                     /tool="antismash"
     gene            1218..2465
                     /locus_tag="INP57_RS25060"
                     /old_locus_tag="INP57_25070"
     CDS             1218..2465
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242371.1"
                     /locus_tag="INP57_RS25060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25070"
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="WP_200072805.1"
                     /sec_met_domain="Fe-ADH (E-value: 8e-116, bitscore: 379.9,
                     seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRVGVGPGSNGDWTGTEQLAGPRVPAEPTTASGQLAKFHAPEIV
                     FGQNALLELGHSAGRLGARHPFLLTDAGVVEAGWAHRACEQLRSVGLHPVVWSDLTPNP
                     KDFEVQAAYDRYVDSGADVIIAVGGGSCVDAAKGVAILSGNGGHILDYEGVDEVVKPIP
                     PLVMVPSTSGTGADVSQFCVVTDTFQETKVTIISRTLVPEISIVDPLLLTTMPEWLNAA
                     TGLDALAHAVESYVSRAHNPLTDHHALHAVSLVTENLLRTLVDPQNMQARTAMAQGSLE
                     AGMAFTNAILGATHAMSHQVGGLLDAPHGMINAILLPHVIRFNAAEDPVRYGPLARAAG
                     IDIDRVRADQAAELLADRVRELADSLGVPRGLRGLGVGEHNVASLARTTMRDACLTTNP
                     RTMDQQQAVALFRAAM"
     gene            2473..3834
                     /locus_tag="INP57_RS25065"
                     /old_locus_tag="INP57_25075"
     CDS             2473..3834
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662122.1"
                     /locus_tag="INP57_RS25065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25075"
                     /product="sensor histidine kinase"
                     /protein_id="WP_200072806.1"
                     /transl_table=11
                     /translation="MHDPDRSESPALGKLTGVRSSKHSFYPEYRRSNERLTHTVRALDE
                     ISQALVRSAEGPRALVEAVVTAAADHLQAQWLLLAISGGQLSGMRPSQLLYHHGTFIDD
                     QHDMPRPIRHHLTMVRTSPWELGTCSLNNGLVRAPMMLDDEPVGGIVALAGEDVQLADT
                     DLAIIRVLANQAAVALYNSHLFQTATHLRGRTDQLTAEASRQAEHLVQRQAELEHVQQQ
                     LTAAIQRQVIDEERHRIARELHDSVTQAVLSAGMIVEVCRSELEVLPPPAPALAQRLEP
                     AKELTQQAVAQLRSAIYALHNASDEVPGSLPVLLERLSHVHLPTELEVAVRVEGDAIAL
                     PPAIEHSMLRLTGEALFNTAMHAHADQAVVVLRYRPDRVLLSVGDDGTGDPDVLQRDLL
                     SAGNEHSGYHCGLANMVHRAEEMGGTLSIRRAELGGVLVHLDVPLSPAWRVSDD"
     gene            3827..4507
                     /locus_tag="INP57_RS25070"
                     /old_locus_tag="INP57_25080"
     CDS             3827..4507
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420248.1"
                     /locus_tag="INP57_RS25070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25080"
                     /product="response regulator transcription factor"
                     /protein_id="WP_200072807.1"
                     /transl_table=11
                     /translation="MIEAAQRHEPPRGSQEETVGIMLVDDHAIVRQGLRAVLERETDLA
                     VVCEASSAEEALASVTRYAPTVVLLDLKLSYGDSTEGLRLCEELIRARPNIGVLVFTTF
                     LEDNLVIEALRRGARGYVVKDVDTTELVRSIRAVARDESAFDSRSAAAMAHSISNQDDT
                     PELTPRELGVLQLLARGMNNREIGEQLHISETTVKFHTRNVKRKLSVCSRAEAVYAASK
                     EGLI"
     gene            complement(4522..5430)
                     /gene="mftM"
                     /locus_tag="INP57_RS25075"
                     /old_locus_tag="INP57_25085"
     CDS             complement(4522..5430)
                     /codon_start=1
                     /gene="mftM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662119.1"
                     /locus_tag="INP57_RS25075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25085"
                     /product="mycofactocin oligosaccharide methyltransferase
                     MftM"
                     /protein_id="WP_200072808.1"
                     /transl_table=11
                     /translation="MNQRAGGPLDTLGPSTPGRYDDGLVEVVRGGDHDASDAVTVARTR
                     HFCLHRNGDRLRIGHRIPPEQLDNDLAGLLRHELFAPGWLSSSVDIFERVFTGVVKTTV
                     DDPGRAWSLFYDNTLEHIRRLWTAEPGPRCSAIAEIVPVYRRSLELVPPGRVLDVGSCF
                     GFLAMLLAARAGTEVIAADIDQGSIDLLRRMARQRRTPVGTLVCDAARIPLPDGAVDTV
                     LAVHLLEHLEPVHGAAVLAEALRLARERVVIAVPFEPEPTAAYGHVRTFDLPALEELGR
                     GAGAHFRVEEHHGGWLVIDRR"
     gene            complement(5427..6944)
                     /locus_tag="INP57_RS25080"
                     /old_locus_tag="INP57_25090"
     CDS             complement(5427..6944)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242005.1"
                     /locus_tag="INP57_RS25080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25090"
                     /product="VWA domain-containing protein"
                     /protein_id="WP_200072809.1"
                     /transl_table=11
                     /translation="MEFTILRFARSLRARNVRISVSEVVDALRALCAPGVLAQRPAFRE
                     ALRSALIKDHRDDPVFDELFHIVFVEAPAAAVSEGREGRSQGGGEPLEDFVLGDVEQRP
                     PRADMESPDAAVGDLFDDNDLVERYSPHPELNPISMSSDSEDELMLSQDDVASIGNSDH
                     VQLETDRMGGTPPPGRLSAANAHRIDADLSDQQQDALLGWLSPLTDPTSAHDDGDLDDA
                     EELRARLSGLLNNLPEALEQHIARLLAAGHSASEVDDPSCSDHVDQGERDELERSLDQL
                     ARSLRGGLTSRRVVSPRGRIDSARTMRRNMRFDGVPFRPVTVRRREDKPRLVVLADVSL
                     SVRATARFTLHLVHGLQNQFRKVRSFAFVAEIVEVTALFSAHPAEHALGLIFDGHTLDV
                     DADSDYGRVLADFRADFRDAIDSRTTVVILGDGRNNGRPANLPGLNEISRHCKELIWLT
                     PEPRYSWGLGGCDLPHYAEHCDRVQVVRDLTGLRRTAERMAAEVTGR"
     gene            complement(6955..8115)
                     /locus_tag="INP57_RS25085"
                     /old_locus_tag="INP57_25095"
     CDS             complement(6955..8115)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662117.1"
                     /locus_tag="INP57_RS25085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25095"
                     /product="MoxR family ATPase"
                     /protein_id="WP_200072810.1"
                     /transl_table=11
                     /translation="MNPTPSYEFDSVEHTISRLAEVGYLADRRLATTVYLLTRLVKPVL
                     LEGPAGVGKTELAHALADSTGRRVVRLQCYEGQDESRALYEWDYGKQMLYTQMLREKIG
                     DIVESSTGIAEAVEQIGSHDDVFFSERFLSSRPLLEAIRSEAPVVLLVDEIDRADEALE
                     AVLLEVLAEGQISIPEIGTFVARHAPYVVLTSNNTRDLSAALKRRCLHLFLDYPDADRE
                     LQIIQSKGTGLGDAVAEHLVQVVRGLRELDLRKPPSISEAIDWARTLAVLGISELGTAE
                     LDETLSVVLKYDKDLARAREQLAALVQGEIPEPASVPAQVDAATEHAPADREGRTVRSA
                     KDHPGRHGPGTYGERSQPAVDGQPARPRVSTGQGERSFTSKFRRRG"
     gene            complement(8196..9488)
                     /gene="mdo"
                     /locus_tag="INP57_RS25090"
                     /old_locus_tag="INP57_25100"
     CDS             complement(8196..9488)
                     /EC_number="1.1.99.37"
                     /EC_number="1.2.98.1"
                     /GO_function="GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_process="GO:0046170 - methanol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mdo"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332348.1"
                     /locus_tag="INP57_RS25090"
                     /note="This methanol dehydrogenase is considered a
                     nicotinoprotein, since its NADP cofactor remains is not
                     dissociable, but instead remains permanently bound. A
                     member of this family has been shown to act as a
                     formaldehyde dismutase, able to convert two molecules of
                     formaldehyde (plus one water molecule) into one of methanol
                     and one of formate, with no net change in its redox state.
                     More recently, it was shown in Mycobacterium smegmatis that
                     this enzyme is critical to ethanol utilization, for which
                     the biosynthesis of the cofactor-like electron carrier
                     mycofactocin is also required; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="INP57_25100"
                     /product="NDMA-dependent methanol dehydrogenase"
                     /protein_id="WP_200072811.1"
                     /sec_met_domain="Fe-ADH (E-value: 8.6e-108, bitscore:
                     353.5, seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQVEELFKPFPLKEFHPVPRALLGPGSHEMIGPEALKIGMRRPLV
                     MTSGLRGTDIVHKIFESLKYHGLDPVLYDQVESNPKDYNVMDSVQLYQENKCDGFVSIG
                     GGSSHDACKGARISVAHDGRNVNEFEGFNKSENSKNPPHIAVSTTAGTGSETSWAYVIT
                     DTTTDPDNPHKYVAFDDHSVSTLAIDDPVLYYDLPTHFSAQCGFDVLAHASEPYVARLD
                     FTPSMGQARHAAELVGQHLRTATWNGQNLAAREGMMYAQYIAGQAFNSGGLGVVHSISH
                     AVSAFYDTHHGLNNAIALPRVWAWNLPVAYKQYAKLAEALGVDTHGMTDVKAADAAVDA
                     AIRLLRDVGIPEKLTDITQDTHEKNRLGQGPTKFYQKRTAIKGDKEDVDRVSRHILGDS
                     CTPGNPKETTFETVRPVVEHAFNGDLEDLVS"
     misc_feature    complement(9680..9691)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 16.9"
                     /tool="antismash"
     misc_feature    complement(9773..9788)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.9"
                     /tool="antismash"
     misc_feature    complement(9898..9912)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.78"
                     /tool="antismash"
     gene            9907..10011
                     /gene="mftA"
                     /locus_tag="INP57_RS25095"
                     /old_locus_tag="INP57_25105"
     CDS             9907..10011
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947188.1"
                     /locus_tag="INP57_RS25095"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="INP57_25105"
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_200072812.1"
                     /transl_table=11
                     /translation="MNEQNSSEQPPAEDVVEDDMLVEEVSIDGMCGVY"
     gene            10001..10309
                     /gene="mftB"
                     /locus_tag="INP57_RS25100"
                     /old_locus_tag="INP57_25110"
     CDS             10001..10309
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229645.1"
                     /locus_tag="INP57_RS25100"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="INP57_25110"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_200072926.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 1.3e-24,
                     bitscore: 79.5, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTEDRSSGVPVPEDSSEFHPDRAYRLSADVALRAEHFGALAYHF
                     GTRKLSFLKTPQLVELVTNLHQHPSARSAVSHLPGAERASYLRALAGLAGARMIVPA"
     aSDomain        10064..10297
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_INP57_RS25100_Mycofactocin_RRE.1"
                     /evalue="2.50E-25"
                     /identifier="RREFam016.1"
                     /label="INP57_RS25100"
                     /locus_tag="INP57_RS25100"
                     /protein_end="99"
                     /protein_start="21"
                     /score="79.5"
                     /tool="antismash"
                     /translation="DRAYRLSADVALRAEHFGALAYHFGTRKLSFLKTPQLVELVTNLH
                     QHPSARSAVSHLPGAERASYLRALAGLAGARMI"
     gene            10385..11563
                     /gene="mftC"
                     /locus_tag="INP57_RS25105"
                     /old_locus_tag="INP57_25115"
     CDS             10385..11563
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983863.1"
                     /locus_tag="INP57_RS25105"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="INP57_25115"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_200072918.1"
                     /sec_met_domain="PTHR43273 (E-value: 2.5e-39, bitscore:
                     128.4, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 7.1e-08, bitscore: 26.0,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 2.6e-26, bitscore: 86.2,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 1e-09, bitscore: 31.4,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.3e-15, bitscore:
                     50.8, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 4.2e-07, bitscore: 23.6,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLVEQFKSGLASPICLTWEWTYACNLSCAHCLSSSGRRDPDELG
                     TDEIKSVIDELERMQVFYVNIGGGEPTVRPDFWELVEYAVDHHVGIKFSTNGYRITQER
                     ARWLAEADYVDVQISLDGATPEVNDAVRGPGSYTTAMRAMENLAAAGMRGFKISVVMTS
                     QNVGQLDEFKRIADHFDAQLRITRLRPSGRGADVWDELHPTAAQQRRLYDWLVAHGEDV
                     LTGDSFFHLNALGSDPLPGLNLCGAGRVVCLIDPVGDVYACPFAIHDAFHAGNIREAGF
                     GPVWRDSELLNDLRRPQTGGACVSCSAYDACQGGCMAAKFFTGLPLDGPDPECVKGHGE
                     AALARVDAQQAPRPAQDHSKPSAKRQQVMLGLPGLRQRPDRACDQSPLAGSS"
     gene            11617..12783
                     /gene="mftD"
                     /locus_tag="INP57_RS25110"
                     /old_locus_tag="INP57_25120"
     CDS             11617..12783
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418891.1"
                     /locus_tag="INP57_RS25110"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="INP57_25120"
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_200072813.1"
                     /sec_met_domain="TIGR03966 (E-value: 4.8e-215, bitscore:
                     706.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRGWFETVAEAQRRARKRLPASVYSALIAGAEQGSTLEDNQAAF
                     TELGFAPHAADLYGQRDLGTTVMGQSLSLPVLISPTGVQAVHPDGEVAVARAAAARGTA
                     MGLSGFASKPVEEVVRANPQTFFQTYWVGSRDDIERRMQRAREAGAIGLIATTDWSFSH
                     GRDWGSPAIPERMNLRTKLKYAPQALTHPGWFAEFAKRRQIPDLTVPNMQQGDDAAPTF
                     FGAYGTWMFTPPPSWEDITWMRKQWDGPFMLKGICRVDDAKRAVDAGVTSISVSNHGGN
                     NLDGTPASIRALPAITDAVGDQVEVLLDGGVRRGSDVVKALALGARAVMIGRAYLWGLA
                     ANGQAGVENVLDILRGGIDSALLGLGHRSVHELGRSDLLVPPGFARGL"
     gene            12990..13928
                     /locus_tag="INP57_RS25115"
                     /old_locus_tag="INP57_25125"
     CDS             12990..13928
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF026980.5"
                     /locus_tag="INP57_RS25115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25125"
                     /product="toxin glutamine deamidase domain-containing
                     protein"
                     /protein_id="WP_200072814.1"
                     /transl_table=11
                     /translation="MSAAAQECANRIEEAKGMRFQEPPGTLDLIGQGRDFIRENKDVLK
                     NISEALKIVSAGLAVVGLALQAIPVVGNAVGGAFLVAAGITGGVALAIDAGIYAATGEG
                     SLTSILVDTALTVIPIGKLAKLGKAAFSMAKDWRFLARPTSHISEPAIHAGSANPAMGR
                     WFLRDQHSWMSGEKAINAPRFNASTPGYVENCSNNVATVEHRLNGVEVSTTPLHNPRWP
                     DPAALGNPNARFQDVASYDDITKDMHARGAGSRGVVYINRPGNPALFIKPTAHVFNVPH
                     GPHGVEFLDGQTGALAELEQHVDRIGYMPYR"
     gene            13959..14258
                     /locus_tag="INP57_RS25120"
                     /old_locus_tag="INP57_25130"
     CDS             13959..14258
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="INP57_RS25120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="INP57_25130"
                     /product="hypothetical protein"
                     /protein_id="WP_200072815.1"
                     /transl_table=11
                     /translation="MVSKEDANRAAEQVLAEVYGDDAVGATIVIEGIGEVPNACIVAFD
                     LADNNTDDPTQLPMVRNIIVPKDGTPAHLPPTALPIPKYLSMVESGEWNWVGAP"
     gene            complement(14441..15460)
                     /locus_tag="INP57_RS25125"
                     /old_locus_tag="INP57_25135"
     CDS             complement(14441..15460)
                     /GO_function="GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013087.5"
                     /locus_tag="INP57_RS25125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25135"
                     /product="methyltransferase"
                     /protein_id="WP_200072816.1"
                     /transl_table=11
                     /translation="MTSESHPTPPPTAQISRLMAGFQVSQALYAVAKLDVPTMLANDGP
                     LPVNVLAERAGADPGALHRLIRSLTPLGVFSSEGDRVRVTELGAILSREHPSSMYNAIC
                     YWMETHYLPFSELLTAARTGRPAADVHLGEPFFDWIAADSDRAELQNRAMTEFSSSLRT
                     GMFDGYVLPEGKVVADIGGASGNVLAELLGADPDRLGIVFDLPAIVSGARDSLARNGLA
                     DRVEVVAGDFFESVPTADVYVLSYILHDWDDESSLRILGSIAAAADSGARVLLLEGVVP
                     ADDLPHLSKMMDLTMLGMLTGQERSAEEYRVLLDRAGFTLDRIVETPTPYSIVEATLR"
     gene            complement(15666..16448)
                     /locus_tag="INP57_RS30440"
                     /old_locus_tag="INP57_25140"
     CDS             complement(15666..16448)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019349.5"
                     /locus_tag="INP57_RS30440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25140"
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="WP_200072817.1"
                     /transl_table=11
                     /translation="MTSKRAGDVSAASGSPADSAAQAIPDTSAAATSPRAISGYQAIVD
                     HLRREMTLGRIRPGDRLPPERQLAEQLGVARDTLRQALRILEGSGQIAIRRGARGGAFV
                     QASMVEAWRIRQDVRERAGAIVALVEFRAVIDSAAARFAAERRSDEDLAAMEQAQAELE
                     ASEALHDSRNADTAFHLAVARASGNHELAVAVEDARVKMFAPVDLLGFQFLKGSCVQGH
                     ALVLAAVRDRDPDRAAVEMTRHLAATREEFELLLTEEL"
     gene            complement(16468..17910)
                     /locus_tag="INP57_RS25135"
                     /old_locus_tag="INP57_25145"
     CDS             complement(16468..17910)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012398.5"
                     /locus_tag="INP57_RS25135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25145"
                     /product="aldehyde dehydrogenase"
                     /protein_id="WP_200072818.1"
                     /sec_met_domain="Aldedh (E-value: 1.9e-106, bitscore:
                     349.5, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTFPSPCTWIDGRAEQSRTDDGASLRDPNTGAELAPSRSSSSEQI
                     DRAVGATAGAHADGRWSSLGAERRAPFLRALADGLDARADEIARLDAINSGVPISVTRL
                     FASSNGDTVRSAADRALALGDAAALPADGRDVRIHRVPWGPAALLMPWNAPSAMAVKKL
                     GFALAAGASAVMKPSPASPWSALLVAEAAAEAGIPDGVVNLVLGGAEAGSRLVEDERIA
                     AISMTGSTPTGRAIAAAAAPRFAKLRLELGSNNPAIVLPDADVEVTAKALAGGAMKLSG
                     QWCEAPRRVLASRAVRDRLVEGLRAELATLRIGSSLDDTTTLGPVAFEGRRQQLTAQRD
                     VLAARGATIVETGLTPDEGFFFPPTLAVGEAVEPDSEIFGPLLTIEAFDEAEDAVRRAN
                     SGQVGLAGYVFSEDIDEAGALGARLVAGEVKVNGTSVLDMSPHSAQSFFGSSGIGGHGD
                     AELLEFFLGKRIVGTDAPGLPL"
     gene            complement(17916..19286)
                     /locus_tag="INP57_RS25140"
                     /old_locus_tag="INP57_25150"
     CDS             complement(17916..19286)
                     /EC_number="6.3.1.-"
                     /GO_function="GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012348.5"
                     /locus_tag="INP57_RS25140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25150"
                     /product="glutamine synthetase family protein"
                     /protein_id="WP_200072819.1"
                     /transl_table=11
                     /translation="MTPLLDPLQLEGAGIHTVIVATPDLQGRLVGRRVPAERFPGILEC
                     GVEICTCAWAWDVEQSFDLIGAGVLAVCSMHNGAPDVALIPDVGTLRRAAWLQGVAICM
                     ADPVWPTTGEPLDISPRVLLKKELARYRDMGYSPQAGTELEFYLFANNPRALRKSDFRD
                     DLDPTTLTPSDFMIHEGNGYEPFFQKLRRDLRDSGIEVEAGQSEWGTGQWEMTFAYGDP
                     LEMADRHALYKLAVRDSAVAAGMSVTFMARPLNDQPGSSCHVHLSLLDDAGGSVFWNAD
                     AEHRMSDTLRSAVAGMLEHTPAFMAWYAPTVNSYRRTNSQEIAGYGRTWAVQNRSVSAR
                     IVGHTSSALRVEYRIPGADTNPYLTLGGVLASARDGIARSATPPAMTVGNAYDGEIDAA
                     MPTQLAEAAEMFATSDFVRRLIGEENTSHFRHLAELEWRVYQSTVTQWDLQRYFDRI"
     gene            complement(19283..20053)
                     /locus_tag="INP57_RS25145"
                     /old_locus_tag="INP57_25155"
     CDS             complement(19283..20053)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (8-121). E-value: 2.6e-08. Score:
                     25.9. Matches aSDomain:
                     nrpspksdomains_INP57_RS25145_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /locus_tag="INP57_RS25145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25155"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_200072820.1"
                     /sec_met_domain="adh_short (E-value: 9e-31, bitscore:
                     100.4, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSERHAGRSVFITGAGGGIGRETALRLAGEGARIMATDVNGDAAR
                     KTAELVEADGGTALSAAVDVRERSGIRAAAEAAAAAWGSLDLLVNNAGLVTPHSLSDLT
                     DDAWDLVLDVNLKGQFLVAQEVAPHLDAAGGGAIVNLSTVEASVVVTSGSTAQPHYNAS
                     KGGVPALTKALAVELAPKNIRVNCVAPGPIATDFFSYEDVTSPEAMEFLGQRLLVRRVG
                     TPADVASAISWLLSDEAAWIDGIELPVDGGWLTR"
     aSDomain        complement(19691..20029)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_INP57_RS25145_PKS_KR.1"
                     /evalue="2.60E-08"
                     /label="INP57_RS25145_PKS_KR.1"
                     /locus_tag="INP57_RS25145"
                     /protein_end="121"
                     /protein_start="8"
                     /score="25.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="SVFITGAGGGIGRETALRLAGEGARIMATDVNGDAARKTAELVEA
                     DGGTALSAAVDVRERSGIRAAAEAAAAAWGSLDLLVNNAGLVTPHSLSDLTDDAWDLVL
                     DVNLKGQFL"
     CDS_motif       complement(19955..20026)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_INP57_RS25145_0001"
                     /evalue="9.00E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="INP57_RS25145"
                     /protein_end="33"
                     /protein_start="9"
                     /score="18.3"
                     /tool="antismash"
                     /translation="VFITGAGGGIGRETALRLAGEGAR"
     gene            complement(20050..21021)
                     /locus_tag="INP57_RS25150"
                     /old_locus_tag="INP57_25160"
     CDS             complement(20050..21021)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019342.5"
                     /locus_tag="INP57_RS25150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25160"
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="WP_200072821.1"
                     /transl_table=11
                     /translation="MVVEDVGAAGKPPGDVGYVLLAIVVLAPGLGAVQALLVKARDPAD
                     FALSAQELDHERHREFDPMNPTRYATAAAASPLIGLTGRRKSAAIMTAPRGFSDAPIDV
                     YFAEFATSVQGAGALAVHLPLDGDARALVARLDGLVLVGGDDVDPRRYGQAPGPFTTVV
                     DPACDQFETDLVTAALERDIPILGVCRGQQILNVVLGGTLQQHLELGEGESHGSYAYPR
                     AHRVHDVEFTEGSTHAAVYGTRTRVNSFHHQAVDRPGDGVTVVGRAPDGTAEAIEIAGT
                     SAIGVQWHPETFGNDPIFTWLARTASAKAGLTRNDNAQEVAR"
     gene            complement(21051..21197)
                     /locus_tag="INP57_RS25155"
                     /old_locus_tag="INP57_25165"
     CDS             complement(21051..21197)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="INP57_RS25155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="INP57_25165"
                     /product="hypothetical protein"
                     /protein_id="WP_200072822.1"
                     /transl_table=11
                     /translation="MSVQGEYRLSSTLGPRAVTFMPIGFLVGNGVGLPVMRLRRSPARW
                     ASP"
     gene            complement(21428..22648)
                     /locus_tag="INP57_RS25160"
                     /old_locus_tag="INP57_25170"
     CDS             complement(21428..22648)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228568.1"
                     /locus_tag="INP57_RS25160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_25170"
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="WP_200072823.1"
                     /transl_table=11
                     /translation="MTSAREPVQEGLRPVRAEAGETVEVRFTVNGTPAVLNLPARVTLA
                     DALRDHLGLTGTHVGCEHGVCGMCTVLVDGEAVRACLLFACQLDGSEVVTVEGLGRPDD
                     LHPLQESFGRNHALQCGFCTPGFLLSSYDLLSNEPEVTDEELPAELSGVICRCTGYRNI
                     MTAVSETRAAHPEGIPEPGNCARRALVGRSGGQSATVTEENAEPSGEPAGNVEIVLPEG
                     DPTIAVQVDTALELPFDQVWRVLDDVHVLAGCLPGAELTEELGDQLYAGRARVAVGPIK
                     LSFHGVARVLEQDRETGTIRVLAQGQDTGGAQTQADITLRAHATAAGTELRADAAVYLT
                     GRIAQFGRALAGDISRRMFEQFADSVRQAATTGEAPQRSHAAPSALRMMLGTLLDRIKR
                     IGRLRGK"
ORIGIN
        1 ctccacaaac ggacccccga cgcatcatcc ggcctccacg ccaccgcact cccatggtcg
       61 ggcgtcactg accaccaccc tcgatgatct ggcgggcccc gccgttcggc acgaccaaga
      121 acgtggccat ctccgtcgca cggcgaactc aacacctcga ccgcaagccc acgcgctgca
      181 cttcacaggc acggatgcag gggagatgag aatgagaacc attttcgata agcttccgtc
      241 atgcggcata cggaaggatt cacggcggtg gcgtgcgggg ttgatccctg tccggctggg
      301 ggtgtcgacc cgctggcagc gctccgatta gccgtccggg aatgcgagca cggcgtgctg
      361 gtccgaagcg gtgggtgccc gctggcactc ctgccacgga cgagcggcac cccagcctgc
      421 acgacatcgc agccgggggc ggggccgttc gttctcgtgc aaccgtgcga cgccgcccgc
      481 cgagcgagcg gcgaggtggt ggtcgcgggg ccgttgcacg aatcggccga cgtgatcgac
      541 ctgtgccact ggctcaccga tgggctgctc accggcacgc ctctccctgg ccacctccgg
      601 ccggcgttcg tcatcagtac cgatcgccgc tcgtagcctc tcccgacgat cagtacgcac
      661 ccccgggctc acgccagctg ccgcggtttc gacatgcagg gacggcccgg tatttccatg
      721 ctcaatcgcg gaaaacctcg ccaccgaagt ggcgaccgcg gacgaagctc gccacgtgcg
      781 acaacgacca ccccgaccgc cccagacgac ctctgtgtcc actgtggatg acttctgcac
      841 tcgcagcggc gctgcgactc tcgatgtgcc caagatgagc cacggcgcgt accgccgcag
      901 ttccggttcc gcgctccggt ccctgcccag ttggacaggt gcaggcccga tcgactgggg
      961 aggttacgtc cccgtgagag acagattacg gtcgggactt gtcgaggtca ctgtcgtagc
     1021 gctgctagtg caccgaggag aaccgctgtg gaccgttacg tggtggaacc gaacttcccc
     1081 gatgccgggg ggctttccga agacggccta cgcacactgt cggcggcgaa cgaggtactc
     1141 cgatccacgc actacgacct gcagctgctc gtgcgagtga cctcacctga cgagccgtcc
     1201 cagaccgcga gcgcttgatg ccccgggttg gcgtgggccc aggctccaac ggcgactgga
     1261 ctgggacgga gcaactggcg ggacctcgtg tgccggctga gccgacgaca gcgagcggac
     1321 agctcgccaa gtttcatgcg cccgagatcg tgttcggcca gaacgcgctg ttggaactcg
     1381 gacacagcgc ggggcgcctc ggagcgcggc acccattcct gctgaccgac gcaggtgtgg
     1441 tggaggcagg ctgggcgcat cgagcgtgcg agcagctcag gtcggtcggg ctgcaccccg
     1501 ttgtgtggtc cgacctgacc cccaatccca aggacttcga ggtccaggcg gcatacgacc
     1561 ggtacgtcga ctccggtgcg gacgtgatca tcgcggtagg gggtggctcc tgcgtggacg
     1621 ccgccaaagg cgtcgcgatc ctgtccggca acggcggcca catcctcgat tacgaagggg
     1681 tcgacgaggt cgtcaagccg attccaccac ttgtgatggt gccctcgact tccggcacgg
     1741 gagccgacgt ttcccaattc tgcgtggtca ccgacacctt ccaggagacc aaggtgacca
     1801 tcatcagccg caccctcgtt cccgagatat cgatcgtcga cccgcttctg ctgaccacga
     1861 tgccggagtg gctcaacgcc gccaccggcc tcgacgctct tgcccacgcc gtcgagtcct
     1921 acgtctcccg tgcgcacaac ccgctcaccg atcaccacgc gttgcacgcg gtcagcctgg
     1981 tgaccgagaa tctcttgcgc acgctcgtcg atccccagaa catgcaggca cgaacggcga
     2041 tggcccaagg gtccttggaa gccggcatgg cctttaccaa cgcgatcctt ggcgccaccc
     2101 acgcgatgag ccaccaagtg ggagggctcc tggacgcgcc gcacggaatg atcaacgcca
     2161 tcctgctgcc ccacgtcatc cggttcaacg ccgcggaaga ccccgtccgc tacgggcccc
     2221 tggctcgtgc agccgggatc gacatcgacc gggtgcgggc ggaccaggcc gccgagctgc
     2281 tcgccgaccg cgtgcgcgaa ttggccgact cgctcggcgt gccgcgagga ctgcgcgggc
     2341 tgggagtcgg cgagcacaac gtcgccagcc tggcccggac gacgatgcgg gacgcttgct
     2401 tgacgaccaa cccccggacc atggaccagc agcaggccgt ggcgctgttc cgcgccgcca
     2461 tgtgagggcc ccatgcacga cccggaccgc tccgaatcgc ccgcgctcgg gaagttgacc
     2521 ggagtccgtt cgagcaagca ctcgttctac ccggagtacc ggcgctcgaa cgaacggctg
     2581 acgcacaccg tccgcgcgtt ggacgagatc tcgcaggcac tcgtgcggtc cgcggaaggg
     2641 ccgcgcgcgc tggtggaagc cgtggtcacc gcggcggccg atcacctgca ggcgcagtgg
     2701 ctgctgctgg cgatctccgg cggccagctc agcgggatgc ggccgagcca gttgctctac
     2761 caccacggga cgttcatcga cgaccagcac gacatgccgc ggccgatccg ccatcacttg
     2821 accatggtgc gcacctcccc gtgggaactg ggcacctgct cgttgaacaa cggactggtt
     2881 cgcgcgccga tgatgctcga cgacgaaccg gtcggcggga tcgtcgcgct cgcgggcgag
     2941 gacgtgcagc tcgccgacac ggacctggcc atcatccgcg tgctggccaa ccaagcggcg
     3001 gtggcgctgt acaactcgca tctgttccag accgcgacgc acttgcgggg ccgcaccgac
     3061 cagctcactg cagaggcgtc ccggcaagcc gaacacctcg ttcaacggca agcggaactg
     3121 gagcacgttc agcaacagct gaccgcggcc atccaacgcc aagtgatcga cgaagagcgc
     3181 caccgcatcg ccagggagct gcacgacagc gtcacgcaag ccgtgctcag cgccggaatg
     3241 atcgtcgagg tttgccgttc cgagctggag gtccttccgc cgcctgcccc ggcccttgcc
     3301 caacggctgg agccggccaa ggagctcacc cagcaagccg tcgcgcagct gcgatcggcg
     3361 atctacgcgc tgcacaacgc cagtgacgaa gtcccggggt cgctaccggt gctgctggaa
     3421 cgactctcgc acgtgcacct gcccaccgag ttggaggtcg cggtgcgcgt ggaaggcgac
     3481 gcgatcgcac tgccgcccgc gatcgagcac tcgatgctgc gactgaccgg cgaggcgctg
     3541 ttcaacacgg cgatgcacgc ccacgccgat caagccgtgg tcgtcctccg gtaccggccc
     3601 gatcgggtcc tgctgtcggt cggcgacgac ggaaccggcg acccggatgt tctgcagcgc
     3661 gacctgcttt ccgccggcaa tgagcacagc ggataccact gcggtttggc gaacatggtg
     3721 caccgagccg aggagatggg cggcacgctg agcattcgac gcgcggagct cggcggcgtg
     3781 ttggtgcacc tggatgtccc gctttccccg gcatggaggg tttccgatga ttgaggcagc
     3841 acagcggcac gaaccgcccc ggggctcgca ggaggagacc gtggggatca tgctggtcga
     3901 cgaccacgcc atcgtccggc aagggcttcg cgccgtgctg gagcgagaaa cggatctggc
     3961 ggtggtgtgc gaggcttcca gcgccgagga agcgctggca tccgtgacgc ggtacgcccc
     4021 caccgtcgtc ctgctcgacc tgaagctctc ctacggcgac tccaccgaag gcttgcggtt
     4081 gtgcgaagaa ctcatccgcg cacgtcccaa catcggcgtg ctcgtgttca cgaccttcct
     4141 cgaagacaac ctcgtgatcg aggcacttcg gcggggggcg agaggttacg tggtcaaaga
     4201 tgtcgacacc accgagttgg tccggtccat ccgcgcggtc gcgcgcgacg agagcgcgtt
     4261 cgactcgcgg tccgcagctg cgatggcgca ctcgatcagc aaccaggacg acacgcctga
     4321 gctgacaccc cgagagctag gggtgctgca gctgctcgca cgcggtatga acaaccggga
     4381 gatcggtgag cagctccaca tctccgagac gacggtgaag ttccacaccc gcaacgtcaa
     4441 gcgcaagctc agcgtgtgca gccgagcgga ggccgtctac gccgcgagca aagaaggatt
     4501 gatctgaaca ccgacgccgc atcagcggcg atcgatgacc aaccagccgc cgtggtgctc
     4561 ctccacccgg aaatgtgctc cggcgccgcg gccgagttct tccaacgccg gtaggtcgaa
     4621 cgtgcgtacg tggccgtagg ccgcagtggg ctccggctcg aacgggacgg cgatgaccac
     4681 ccgttcccgc gcgaggcgaa gtgcctcggc caggaccgcg gcgccatgga ccggttcgag
     4741 atgttcgagc aggtgcacgg cgagcaccgt gtccacggcg ccgtccggca acgggattcg
     4801 ggctgcgtcg cagacgaggg tgccgacggg cgtgcggcgc tgccgcgcca tccgtcgcag
     4861 cagatcgatg cttccctggt cgatgtccgc ggcgatcacc tcggtaccgg cgcgcgcggc
     4921 caacagcatc gccaggaatc cgaagcagga cccgacgtcg agaacgcgcc ctggtggcac
     4981 gagttccaga ctgcggcggt agaccggaac gatctccgct atcgcggagc agcggggtcc
     5041 cggttcagcg gtccacagcc gccgtatgtg ctcgagggtg ttgtcgtaga acagcgacca
     5101 ggctcgcccg ggatcgtcca cagtggtctt gacgacgccg gtgaacacgc gctcgaagat
     5161 gtcgacgctg ctgctgagcc aacccggggc gaacaactcg tggcggagca gtccggcgag
     5221 gtcgttgtcg agctgctccg gtgggatccg gtggccgatc cggaggcggt ccccgttgcg
     5281 gtgcagacag aagtgccgcg tgcgggccac cgttaccgca tcggatgcat cgtggtcgcc
     5341 gccgcgcacg acttcgacca gcccgtcgtc gtaacgtccc ggggtcgaag ggccgagcgt
     5401 gtcgagcggc ccaccggctc gctggttcat cggccggtca cctccgcggc catgcgctcc
     5461 gccgtgcggc gcagcccggt caggtcacgc accacttgga cccggtcgca gtgttcggcg
     5521 taatgcggca ggtcgcagcc cccgaggccc cacgagtacc gcggttccgg ggtcagccag
     5581 atgagttcct tgcagtgccg ggaaatctcg ttcaacccgg gtagattggc cggacgcccg
     5641 ttgttgcgcc catccccgag gatcacgacc gtggtgcggc tgtcgatcgc gtcgcggaaa
     5701 tcagcgcgga agtcggccaa gacgcgtccg tagtcggagt cggcgtcgac gtcgagggtg
     5761 tgcccgtcga agatgagccc gagcgcgtgt tcggccgggt gcgcgctgaa cagcgcggtc
     5821 acctcgacga tttcggccac gaacgcgaag ctgcgaacct tgcggaactg gttctgcaag
     5881 ccgtgcacca agtggagcgt gaaccgagcg gtggctcgca cggacaggct cacgtcggcg
     5941 agcaccacca accgcggctt gtcctcgcga cgccgcacgg tgaccggccg gaacggcacg
     6001 ccgtcgaagc gcatgttgcg acgcatcgtg cgcgccgagt cgatgcgtcc tcgcggagaa
     6061 accacgcgcc ggctggtgag cccaccgcgg aggctgcggg cgagttggtc gagtgatcgc
     6121 tcgagttcgt cacgctcacc ttggtcgacg tggtccgaac aggacggatc atcgacctcg
     6181 gaagcggagt gcccggccgc cagcaggcgg gcgatgtgtt gctcgagcgc ttccgggagg
     6241 ttgttcaaca gcccggacag ccgggcgcgc aactcctcgg cgtcatcgag gtctccatcg
     6301 tcgtgcgcac tggtggggtc ggtgagcggg ctgagccatc ccagcagggc gtcctgctgc
     6361 tgatcgctga ggtcggcgtc gatgcggtgc gcgttggccg cggacaaccg gccgggcggt
     6421 ggtgtgccgc ccatccggtc ggtctccagc tgcacgtgat cgctgttgcc gatgctggcc
     6481 acgtcgtcct gggagagcat gagctcgtct tccgaatcgg agctcatgct gatcgggttg
     6541 agctccgggt gcgggctgta gcgttcgacg aggtcgttgt cgtcgaacag atcaccgacc
     6601 gctgcgtccg ggctttccat gtccgcccgt ggtgggcgct gctcgacgtc tccgaggacg
     6661 aagtcctcca gcggttcacc gccgccctgg gatcgccctt cgcgtccctc gctcacggcc
     6721 gcggcaggcg cctcgacgaa tacgatgtgg aagagttcgt cgaacaccgg gtcgtcgcgg
     6781 tgatccttga tcaaggcgct ccggagtgct tcccggaacg ccggccgctg cgccaacacc
     6841 ccgggggcgc acagcgcgcg cagcgcgtcg acgacttcgg agaccgatat gcgcacgttc
     6901 cgcgcgcgca acgagcgagc gaaacgcaag atcgtgaact ccatgaccgc tccttcaacc
     6961 gcggcggcgg aacttggagg tgaacgaccg ctccccttgt ccggtgctga cccgcggccg
     7021 cgcgggctgc ccgtcgacgg ccggttggct gcgttcgccg tacgttccgg ggccgtgcct
     7081 gccggggtgg tccttggcgc tgcgcaccgt ccggccctct cgatcagccg gcgcgtgttc
     7141 ggttgcggcg tccacttgag ccggcaccga ggcgggttcg gggatctcgc cctggacgag
     7201 cgccgccaac tgctcgcggg cccgggcgag atccttgtcg tacttgagga ccaccgacaa
     7261 cgtctcgtcc agttcggccg tgccgagctc ggaaatgccg aggaccgcca gcgtgcgcgc
     7321 ccagtcgatg gcctcggaga tgcttggcgg cttgcgcaag tcgagttccc ggagcccgcg
     7381 cacgacctgc accaggtgct cggcgacggc gtcgccgagg ccggttccct tggactggat
     7441 gatctgcagt tcgcgatcgg catcgggata gtcgaggaac aggtgcaggc aacgcctctt
     7501 caacgccgca gacagatcac gggtgttgtt ggaggtcaac acgacgtacg gcgcgtggcg
     7561 ggcgacgaag gttccgatct cgggtatcga gatctggcct tcggccaaga cctccagcag
     7621 cacggcctcc agcgcctcgt cggcgcggtc gatctcgtcg acgagcagga ccaccggggc
     7681 ctcggagcgg atcgcttcga gcagggggcg cgaggagagg aagcgctccg agaagaacac
     7741 gtcgtcgtgc gaaccgatct gctcgacggc ctcggcgatg ccggtcgagc tctcgacgat
     7801 gtcaccgatc ttctcgcgca gcatctgggt gtagagcatc tgcttgccgt agtcccactc
     7861 gtacagggcg cggctttcgt cctgcccctc gtagcactgc aaccgcacca cgcggcgacc
     7921 ggtgctgtcg gccagcgcgt gggcgagctc ggtcttgccg acgccggccg ggccttccag
     7981 cagcaccggt ttgaccagcc gggtcagcag gtacacggtg gtcgccaacc tccggtcggc
     8041 caggtaaccc acctccgcca gccggctgat cgtgtgctcg acggagtcga attcgtagct
     8101 cggcgtggga ttcacggact cgcctttcca cgtggtttcg actggccggt gcccgcccgc
     8161 gcatcggcgg gcgggcacca gaggaggact cgacctcaac tcaccaggtc ttccaggtcc
     8221 ccgttgaagg cgtgctcgac gaccgggcga acggtctcga acgtcgtctc cttggggttc
     8281 cccggcgtgc agctgtcgcc gaggatgtgc cgggagacgc gatccacgtc ttccttgtcg
     8341 cccttgatcg ccgtgcgctt ctggtagaac ttcgtgggcc cctggccgag ccggttcttc
     8401 tcgtgggtgt cctgggtgat gtcggtgagc ttctccggga tgcccacgtc gcgcaggagc
     8461 cggatcgcgg cgtccactgc ggcgtccgcg gccttgacgt ccgtcattcc gtgggtgtcc
     8521 acgcccagcg cctcggcaag cttggcgtac tgcttgtagg ccaccggcag gttccaggcc
     8581 cacacgcgcg gcagtgcgat cgcgttgttc aggccgtggt gggtgtcgta gaacgcgctg
     8641 accgcgtgcg agatcgagtg caccacgccg agcccgccgg agttgaaggc ctgccccgcg
     8701 atgtactgcg cgtacatcat cccttcgcgg gcggccaggt tctgaccgtt ccacgtggcg
     8761 gtgcgcaggt gctggccgac gagctcggca gcgtgccgcg cctgccccat cgacggggtg
     8821 aagtcgagtc gggcgacgta gggctcggag gcgtgcgcga gcacgtcgaa accgcactgg
     8881 gcgctgaagt gcgtcggcag gtcgtagtac agcaccggat catcgatggc cagcgtgctc
     8941 accgagtggt cgtcgaaggc gacgtacttg tgcgggttgt ccgggtcagt ggtcgtatcc
     9001 gtgatcacgt acgcccacga ggtctccgag ccggtccctg cagtggtgga caccgcgatg
     9061 tgcggcgggt tcttcgaatt ctcgctcttg ttgaaccctt cgaattcatt cacgttccgc
     9121 ccgtcgtgcg ccaccgagat ccgcgcgccc ttgcaagcat cgtgggagga cccaccgccg
     9181 atggagacga agccgtcgca cttgttctcc tggtagagct gcaccgaatc catgacgttg
     9241 tagtccttgg gattcgactc cacctggtcg tacagcacgg ggtcgagccc gtggtacttc
     9301 agcgactcga agatcttgtg cacgatgtcg gtgccgcgaa gtccagaggt catcacgagc
     9361 gggcgacgca tcccgatctt cagcgcttcc gggccgatca tctcgtgcga cccaggccct
     9421 agaagtgctc ggggaaccgg gtggaactcc ttgagcggga agggcttgaa cagctcttcg
     9481 acctgcattc gcatctcctc tcgccgcgct ttgccgaacc gtgcgttcac gcggttcgga
     9541 ctacccaagt gatcggcgtc actgaaatct gacgctaggg ctttcagcgc ggcgagcgga
     9601 agacgtcgcg cggcgaaccg acgcttcggc gaggcaagaa cgactgatcg ggccagacgg
     9661 atcctgccta cgtgggcagg aaatcgttgt ccactccgca ggaaacgagc cgcaccgcac
     9721 gtctcggacg gcgaccatcc acctctggta tcgagtggga caggttgcga ccctgaccaa
     9781 ccggacagga aatccgtgcc cgctcgcacg gcgaccgtgc tgcgcggcgc cgactagatt
     9841 cagcacctgt tccgttttcc acccccgctg cgcgctgcga agagcacgac caggaggaat
     9901 tttccgatga atgagcagaa cagttccgag caaccgcccg ccgaggacgt cgtcgaggac
     9961 gacatgctgg tcgaggaagt ctcgatcgac gggatgtgcg gtgtctactg aggaccgctc
    10021 ctccggggtt ccggtcccgg aggattcctc ggagttccac ccggaccgcg cctaccggct
    10081 cagtgccgac gtagcgctcc gcgcggagca tttcggcgcg ctcgcgtacc acttcggcac
    10141 ccgcaagctg tcgttcctga agacaccgca gctggtggag ctcgtgacga acctgcacca
    10201 gcatccttcc gcacgatccg cggtgagcca cctgcctggc gccgagcgag cgagctacct
    10261 gcgcgccctg gccggattgg ccggtgcgcg catgatcgtg cccgcttgag cgcgccccac
    10321 cgcagtcgaa aggtcagcta tgcacgcacc tctgaaaacc agcaccggcg gcacctcgca
    10381 ggtactgccg ctcgtcgagc agttcaagtc cgggttggcg tcaccgatct gcctgacctg
    10441 ggagtggacc tacgcctgca acctgtcctg cgcgcactgc ctgtcctcat ccggtcgccg
    10501 cgatccggac gagctgggca ccgacgagat caagtccgtg atcgacgagc tggagcggat
    10561 gcaggtcttc tacgtcaaca tcggcggtgg ggaacccacc gtgcgcccgg acttctggga
    10621 actcgtcgag tacgcggtcg accatcacgt cggtatcaag ttctccacca acggctaccg
    10681 catcacccag gagcgggcac gctggctggc cgaggccgac tacgtggacg tgcagatatc
    10741 actggacggc gccacgcccg aagtcaacga cgccgtacgc ggcccgggct cctacaccac
    10801 cgcgatgcgg gcgatggaga acctcgcagc cgccgggatg cggggcttca agatctcggt
    10861 ggtgatgacc agccagaacg tcggccagct cgacgagttc aagcgcatcg ccgaccactt
    10921 cgacgcccag ctgcgcatca cccgcctgcg cccgtcggga cgcggcgcgg acgtgtggga
    10981 cgaactgcac cccaccgccg cgcaacagcg ccggctctac gactggttgg tggcgcacgg
    11041 cgaggacgta ctcaccggtg actcgttctt ccacctcaac gccctcggat cggacccgct
    11101 gcccggactg aacctgtgcg gggcgggccg ggtcgtgtgc ctgatcgatc cggtcggaga
    11161 cgtctacgcc tgcccgttcg ccattcacga cgctttccac gcgggcaaca tccgcgaggc
    11221 ggggttcggg ccggtgtggc gggattcaga gctcctcaac gacctgcgac gtccgcagac
    11281 cggcggagcg tgcgtctcgt gctccgccta cgacgcctgc caaggtggct gcatggccgc
    11341 gaagttcttc accggcctgc ccctcgacgg ccccgatccc gagtgcgtca agggccacgg
    11401 tgaggccgcg ctggcccggg tcgacgcgca acaggcacca cgtccggcgc aggaccactc
    11461 caaacccagt gccaagcgac aacaggtgat gctcggactc cccgggctgc gccagcgtcc
    11521 ggaccgcgcc tgcgaccaga gcccgctcgc gggctcgtcc tgagcggccc cgctgactgc
    11581 cccgccccgt tcgccgatgt gaaagaggaa gtgcccatgg gtagaggctg gttcgaaacg
    11641 gtcgccgaag cacagcgccg cgcgcgaaaa cgcctcccgg catcggtcta cagcgccttg
    11701 atcgccggtg ccgagcaggg cagcacgctc gaagacaacc aggcggcgtt cacggagctg
    11761 ggtttcgcgc cgcacgcggc ggatctctac gggcaacgag acctcggcac cacggtgatg
    11821 ggccagtcgc tctcgttgcc cgtgctcatc tcccccaccg gtgtgcaagc cgtgcatccg
    11881 gacggtgaag tggccgtcgc gcgcgccgct gccgcccgcg gtacagccat ggggctgagc
    11941 ggattcgcga gcaaacccgt cgaagaagtc gtccgggcca acccgcaaac cttcttccag
    12001 acctattggg tgggcagccg cgacgacatc gaacggcgga tgcagcgtgc gcgcgaggcc
    12061 ggcgcgatcg gcctgatcgc caccaccgac tggtccttct cgcacggccg ggactggggc
    12121 agccccgcga tcccggagag gatgaacctg cgcaccaagc tcaagtacgc cccgcaggcg
    12181 ctgacgcacc ccggctggtt cgccgagttc gccaagcgac gccagatccc cgacctgact
    12241 gttccgaaca tgcagcaagg tgacgatgcg gcgccgacct tcttcggcgc ctacggcacc
    12301 tggatgttca ccccgccgcc gagctgggag gacatcacct ggatgcgcaa gcagtgggac
    12361 gggcccttca tgctcaaagg catctgccgg gtcgatgatg ccaagcgcgc cgtggacgca
    12421 ggggtcacct cgatctccgt gtccaaccac ggcggcaaca acctcgacgg cacaccggcg
    12481 agcattcgcg cactacccgc gatcaccgac gcagtgggcg atcaagtaga agtgctgctc
    12541 gacggcggag tgcggcgcgg tagcgatgtc gtcaaagcgc tcgcactcgg cgcccgggcg
    12601 gtcatgatcg gtcgcgccta cctctggggc ctggcggcca acgggcaagc gggtgtcgag
    12661 aacgtcctcg acatcctgcg cggcgggatc gactccgccc tgctcgggct cggacaccgc
    12721 tcggtgcacg aactcggccg gtccgacctc ctagttccac ccggattcgc aagagggctc
    12781 tgaaacagca cgtactacgg ccacaacgca ccacgccacc gacctgctca ccagcccgca
    12841 ccaccaacga cgcgctgtgg accagttgta ttcccgccgc gccgaggcca ggatcccgac
    12901 cgggatcatg acgacgaaag cgccgatgaa gaactggccg tcgagctccg cgcggtgccc
    12961 tggtcgcggg caatgggctg cgtaccgaga tgagcgccgc cgctcaggaa tgcgcgaacc
    13021 gcatcgaaga ggccaaagga atgcggttcc aggaaccccc gggcacgttg gatctgatcg
    13081 gccagggacg ggatttcatc cgggagaaca aagatgttct caaaaacatc tccgaggccc
    13141 tgaagatcgt ctccgcggga ctcgcggtcg ttggccttgc cttgcaggcc atcccggtgg
    13201 tcggcaatgc cgtcggagga gcgttcctag tggccgccgg gatcaccggc ggcgtcgcgc
    13261 tggccatcga cgccggcatc tacgccgcca ccggcgaagg cagcctcacc tcaattttgg
    13321 tcgacaccgc gttgacggtc attcccatag gcaagttggc gaaactcggc aaagctgcct
    13381 tcagcatggc caaggactgg cgttttcttg cacgccccac gtcacacatt tccgaaccgg
    13441 caatccacgc cggctctgcg aatccggcca tggggagatg gttcctccgg gatcagcact
    13501 cgtggatgag cggcgaaaag gccatcaatg ctcctcggtt caacgcgagc acgcctggct
    13561 acgtggagaa ctgctcgaac aatgtggcca cggttgaaca tcggttgaac ggggtcgagg
    13621 tctcgaccac accgttgcac aatccgcggt ggccggatcc ggccgcactg ggcaatccca
    13681 atgccaggtt ccaggacgtg gccagctacg atgacatcac caaggacatg cacgcccgcg
    13741 gtgcaggatc acgtggagtg gtctacatca atcgtccggg caacccggcc ttgttcataa
    13801 agcccacggc ccacgtgttt aacgtccccc acggcccgca cggggtggag ttcctcgatg
    13861 gccaaacggg tgctctcgcg gagctggaac aacacgtcga ccgcattgga tacatgccgt
    13921 accggtaggt gcgcgacagg ttgaggagtg cagctggaat ggtttcgaaa gaagacgcca
    13981 accgcgccgc ggaacaagtt ttggcggagg tttacggcga cgatgcggtt ggggccacga
    14041 tcgtcattga gggaatcggc gaggtcccca acgcttgcat cgtggcattc gacctggccg
    14101 acaataacac ggatgatccg acccagttgc cgatggtgcg gaatatcatc gtccccaagg
    14161 acggcactcc cgcacacctg ccaccgacag cgcttccgat cccgaagtat ctgtccatgg
    14221 tcgagtccgg agaatggaac tgggtcggcg cgccataatt ccgaccgcac cctgcccccg
    14281 tgacgattca tgactgctgc ccggtgacat ccagcgggcc agcgcctgaa gatcccggct
    14341 tggtgctggt ggcgctcacc agcccgaaat cgccgcccgg acgtcgccgc gccgcctggt
    14401 tctccccagg cggcgcagcg gtcgtggtgc cgggtgatcg tcagcgcagc gtggcttcga
    14461 cgatggagta cggcgtcggg gtttccacga tgcggtccag ggtgaaaccg gcccggtcga
    14521 gcagcacccg gtattcctcg gcgctgcgct cctgaccggt cagcatcccg agcatggtca
    14581 ggtccatcat cttgctcagg tgcgggaggt cgtccgcggg aacgacgcct tcgagcagca
    14641 gcacccgcgc cccggaatcg gccgccgcgg cgatcgaacc gaggatgcgc aggctcgact
    14701 cgtcgtccca gtcgtgcagg atgtaggaca gcacgtagac atcggcggtc ggaaccgatt
    14761 cgaagaagtc gccggcgacg acctcgaccc ggtccgccaa cccgttgcgg gccaggctgt
    14821 cccgcgcgcc ggacacgatc gccggaaggt cgaagacgat cccgagccgg tccgggtccg
    14881 cccccagcag ctcggccagg acgttgccgc tggcaccgcc gatgtcggcg acgaccttgc
    14941 cctcgggcag gacgtagccg tcgaacatgc ccgtccgcag gctcgagctg aactccgtca
    15001 tcgcccggtt ctgcagctcg gcgcggtcgg aatcggccgc gatccagtcg aagaacggtt
    15061 cgccgaggtg gacgtcggct gccggtcggc cggtgcgcgc ggcggtgagc aactcgctga
    15121 acgggaggta gtgcgtctcc atccagtagc agatcgcgtt gtacatcgac gaggggtgct
    15181 cgcgggaaag gatcgcgccc agctccgtga cgcggacccg gtccccctcg ctgctgaaca
    15241 cgcccagcgg ggtcaaggat cggatcagcc ggtgcagcgc gcccggatcg gctccggcgc
    15301 gctcggcgag cacgttcacc ggcagcgggc cgtcgttcgc cagcatggtc ggcacatcca
    15361 gcttcgccac cgcgtacagc gcctgcgaca cctggaaacc ggccatcagg cggctgatct
    15421 gggcggtcgg cggcggggtg ggatggcttt ccgaggtcat cgatcctccg agttcggggt
    15481 cgcgcgggcg aacttccggc cggctcgcac cggaaagttc cttgatcgac aacctatcgg
    15541 cacccgctgc cgaacgtcca cgcacgtgcg cgaccaacag cccccaacac gccggccgac
    15601 ctgagatgat cttgatgttg ctgcgccacg gcaaccatcc gaagaactgc gatacgcacc
    15661 gctagtcaca attcctcggt gaggagcagt tcgaactcct ccctggtcgc ggcgagatgc
    15721 cgggtcatct ctaccgccgc tcggtccggg tctcgatccc ggacggcggc gagcaccagc
    15781 gcatgcccct gcacgcagga ccctttgagg aactggaaac ccaggaggtc gaccggcgcg
    15841 aacatcttga ccctggcgtc ttcgacggcg accgcgagct cgtggttccc ggaagcacgc
    15901 gcgaccgcga ggtggaacgc cgtgtccgca ttgcgggagt cgtgcagcgc ctcggacgct
    15961 tcgagctcgg cctgcgcctg ttccatcgcg gcgaggtcct cgtcgctgcg ccgttcggcg
    16021 gcgaagcgtg ccgcggcgct gtcgatcacg gcgcggaact cgacgagcgc cacgatggca
    16081 cccgcccgtt cgcgaacgtc ctggcggatc cgccacgcct cgaccatcga ggcctgcacg
    16141 aacgctccac cgcgggcacc gcggcggatg gcgatctgcc cactgccctc caggatccgc
    16201 agcgcctggc gcagcgtgtc ccgtgcgacc ccgagctgtt cggccagctg ccgctccggg
    16261 ggaagacggt ccccgggccg gatacgtccc agcgtcatct cccgccggag atgatcgacg
    16321 atggcctggt agccggaaat ggcccgcggc gacgtggctg cagccgaggt gtccgggatg
    16381 gcctgcgctg ccgagtcggc cgggctcccc gaggcggctg agacgtcacc ggcccgcttg
    16441 ctcgtcacgc tcgcaccccg ctcggactca gaggggaaga ccgggcgcgt cggttcccac
    16501 gatcctcttg ccgaggaaga actctagcag ttcagcgtcc ccgtgccccc cgataccgga
    16561 gctgccgaag aagctctgcg ccgagtgcgg cgacatgtcc agcacgctcg tcccgttgac
    16621 cttcacctct ccggcgacga gccgggcacc gagcgcacct gcctcgtcga tgtcctcgga
    16681 gaagacgtat ccggcgagtc ccacctggcc ggaattcgcg cggcgcaccg catcctcggc
    16741 ttcgtcgaag gcctcgatcg tgagtagtgg gccgaagatc tccgagtccg gctcgacggc
    16801 ttcaccgacg gcgagcgtcg ggggaaagaa gaagccttcg tcgggcgtca gacctgtctc
    16861 gacgatcgtg gcgccgcggg cggcgaggac atcgcgctgc gcggtcagct gctgccgccg
    16921 cccctcgaag gcgacgggcc cgagcgtcgt cgtgtcgtcg aggctggaac cgatccgcag
    16981 cgtggcgagt tccgcgcgca gcccctcgac gagcctgtca cgcaccgcgc gcgacgcgag
    17041 gacccgtcgc ggtgcctcgc accattggcc ggagagcttc atggcgccgc ccgcgagcgc
    17101 ctttgcggtg acctcgacat cggcgtccgg caacacgatc gccgggttgt tcgagccgag
    17161 ctcgaggcga agcttggcga agcggggtgc ggcggcggcc gcgatggcgc ggccggtcgg
    17221 agtggagccg gtcatcgaga tcgcggcgat ccgctcgtcc tcgacgagcc gggatcccgc
    17281 ctccgcaccg ccgagcacca ggttcacgac gccgtcggga atgcccgctt cggcggccgc
    17341 ttccgcgacg agcagcgcgc tccacggcga ggccggtgac ggtttcatga cggcgctcgc
    17401 gccggccgcg agtgcgaaac cgagcttctt gacggccatc gccgagggtg cgttccaggg
    17461 catcaggagg gcggcggggc cccacggcac gcggtggatg cgcacgtccc tgccgtccgc
    17521 gggcagggcc gcggcgtcgc cgagcgcgag cgcccggtcc gcggcggagc gcacggtgtc
    17581 gccgttgctg gacgcgaaca gccgggtcac cgagatcggc acgcccgagt tgatcgcgtc
    17641 gaggcgggcg atctcgtcgg cacgggcgtc cagaccgtcg gcgagggccc gcaggaacgg
    17701 tgcgcgcctc tcggcgccga gcgaggacca gcgcccgtcg gcatgtgcac cggccgtggc
    17761 gccgacggcc cggtcgatct gctcgctcga cgacgagcga ctcggtgcga gttcggcgcc
    17821 ggtgttcggg tcgcgcagcg atgctccgtc gtcggtgcgg ctctgctcgg cgcggccgtc
    17881 gatccaggtg cacggggagg gaaaggtcat gctcgtcaga tccgatcgaa atagcgctgc
    17941 aggtcccatt gcgtcaccgt ggattggtag acgcgccact ccaactcggc gaggtggcgg
    18001 aagtgcgatg tgttctcttc accgatgagc cggcggacga aatcggacgt cgcgaacatc
    18061 tcggctgcct cggcgagctg cgtgggcatg gccgcgtcga tctccccgtc gtaggcgttg
    18121 cccaccgtca tcgcgggcgg tgtcgccgaa cgtgcgatgc cgtcgcgggc ggaggcgagc
    18181 acgccgccca gggtcagata gggattggtg tctgcgccgg gtatccggta ttccacacgc
    18241 agggcggacg acgtgtgccc gacgatccgc gctgagaccg accggttctg caccgcccat
    18301 gtccggccgt accccgcgat ctcctgtgag ttggtgcgcc ggtaggagtt gacggtcggc
    18361 gcgtaccagg ccatgaacgc gggggtgtgc tcgagcatcc cggcgacggc cgaacgcagc
    18421 gtgtcgctca tgcggtgctc ggcgtcggcg ttccagaaga ccgacccacc cgcgtcgtcg
    18481 agcagcgaaa ggtgcacgtg gcaggacgag ccgggctggt cgttgagcgg gcgcgccatg
    18541 aacgtcaccg acatgcccgc tgccaccgcg ctgtcgcgca cggcgagctt gtagagcgcg
    18601 tgccggtcgg ccatctcgag cgggtcgccg taggcgaagg tcatctccca ctgcccggtt
    18661 ccccattcgg actgccccgc ctccacctcg ataccgctgt cccgcaggtc gcgacgcaac
    18721 ttctggaaga agggctcgta gccgttgccc tcgtggatca tgaagtcgct cggcgtgagg
    18781 gtcgtggggt ccaggtcgtc ccgaaagtcg ctcttgcgca gcgcacgagg gttgttcgcg
    18841 aacaggtaga actccagctc ggtaccggcc tgcggcgagt agcccatgtc ccggtacctg
    18901 gcgagctcct tcttgagcag cacgcgcggc gagatgtcga gcggttcgcc ggtcgtgggc
    18961 cacacggggt cggccatgca gatggcgact ccctgcagcc acgcggctcg gcgcagcgtg
    19021 ccgacgtcgg ggatgagcgc cacgtcgggt gcgccgttgt gcatgctgca gacggcgagg
    19081 acgcccgcgc cgatgaggtc gaaggactgc tcgacgtccc aggcccacgc gcaggtgcag
    19141 atctcgacgc cgcactccag gatgccgggg aagcgctccg cgggaacccg ccgtcccacc
    19201 aggcggccct gcaggtccgg ggtcgcgacg atcaccgtgt ggatgcccgc tccttcgagc
    19261 tggagcgggt caaggagagg ggtcatcgcg tcaaccaccc gccgtccacc ggcaattcga
    19321 tgccgtcgat ccaggccgcc tcgtccgaga gcagccacga gatcgccgaa gccacgtcgg
    19381 ccggcgttcc gacccggcgc acgagcaggc gctgtcccag gaactccatg gcctcggggc
    19441 tcgtgacgtc ctcgtaggag aagaagtccg tcgcgatggg tcccggcgcg acgcagttga
    19501 cgcggatgtt cttgggggcg agttcgacgg cgagcgcctt ggtgagtgcg ggtacgccgc
    19561 ccttgctcgc gttgtaatgc ggctgcgcgg tgctgccgct ggtcacgacc acggacgcct
    19621 cgacggtgga gaggttgacg atcgcgccgc cgccggcggc atcgaggtgc ggggccacct
    19681 cctgcgcgac caggaactgg cccttgaggt tgacgtcgag cacgaggtcc caggcgtcgt
    19741 cggtgaggtc ggacagcgag tgcggagtca ccaggcccgc attgttgacg agcaggtcga
    19801 gcgatcccca cgccgcggcc gcggcctcgg cggcggcccg gatgccggag cgctcacgca
    19861 cgtcgacggc ggcggacagc gcggtaccgc catcggcctc gacgagttcc gcggttttcc
    19921 gggcagcgtc gccgttgacg tcggtcgcca tgatcctcgc gccctcgccc gccaggcgca
    19981 gcgcggtctc ccggccgata ccgccgcctg cgccggtgat gaagaccgat cggccggcat
    20041 gtcgttcgct catcgtgcga cctcctgtgc gttgtcgttg cgggtgagcc cggccttggc
    20101 ggacgcggtg cgggcgagcc aggtgaaaat cgggtcgttg ccgaaggtct cgggatgcca
    20161 ctgcactccg atcgcggacg tgcccgcgat ctcgatggcc tcggcggtgc cgtccggggc
    20221 acggccgacg acggtcaccc cgtcgccggg acggtcgacc gcctggtggt ggaaggagtt
    20281 gactctggtg cgcgtgccgt agacggccgc gtgcgtgctg ccctcggtga actccacgtc
    20341 gtggacccgg tgcgcacgcg gataggcgta ggagccgtgc gactcgcctt ccccgagctc
    20401 gagatgctgc tgcagcgtgc ccccgagcac cacgttgaga atctgctggc cccggcaaac
    20461 gccgaggatg gggatgtccc gctccagtgc cgcggtgacg aggtcggtct cgaactggtc
    20521 gcaggccgga tccacgaccg tcgtgaaagg accgggcgcc tggccgtacc ggcgcgggtc
    20581 gacgtcgtcc ccgccgacca gaacgagccc gtcgagtcgc gccacgagcg cccgcgcgtc
    20641 gccgtcgaga ggcaggtgca ccgcgagcgc gcctgcgccc tgtaccgacg tggcgaattc
    20701 ggcgaagtag acgtcgatgg gcgcgtcgga gaatccccgc ggagccgtca tgatcgccgc
    20761 gctcttccgg cgaccggtca gcccgatcag cgggctcgcc gcggcggcag tcgcgtaccg
    20821 agtcggattc atcggatcga actcccggtg tcgttcgtgg tcgagttcct gtgcgctcag
    20881 ggcgaaatcc gcgggatcgc gagctttgac caacagcgcc tgcaccgcgc cgagacccgg
    20941 cgccaggacg acgatcgcga gcagcacgta gcccacgtcc ccaggtggct tgcccgcggc
    21001 gccgacgtct tcgacgacca acgggaggtt gctggcgatc agttgggcgg ctacggcgag
    21061 gcccaccggg ccggcgagcg gcgcaaccgc atcacgggca gaccgactcc gttaccgacc
    21121 aggaagccga tcggcatgaa ggtcacggcg cgcgggccga gggtgctgct cagccggtat
    21181 tcgccctgga cgctcatcgg cgaacctcct tcaggagcgt ttggcccaat aactagacca
    21241 ttcgtccatg ggcgccgaaa acccaaggcg ggagagttga ccgaaccgtt acgcggcgcg
    21301 tgctcgccga cgtccgggcc ctccgccgaa tacttgtcag acgttcggac gacacggccg
    21361 agccgcggtc tcatgcagcc gatcgcgttg ccacagcgtg gaattcgagc caaccgcccg
    21421 cctcggctca cttcccgcga agacggccga ttcgcttgat ccggtccagc agggtgccga
    21481 gcatcatgcg cagcgcgctc ggcgcggcgt gggaccgctg cggtgcttct ccggtggtgg
    21541 cggcctggcg gaccgagtcg gcgaactgct cgaacatgcg tcggctgatg tcgcctgcca
    21601 gcgctcggcc gaactgggcg atgcggccgg tcaggtacac ggccgcgtcg gcgcggagtt
    21661 ccgttcccgc tgcggtcgca tgcgcccgca gcgtgatgtc ggcctgggtt tgcgcccctc
    21721 cggtgtcctg cccctgcgcg agcacgcgga tcgtcccggt ttcccggtcc tgttcgagta
    21781 cccgggcgac cccgtggaac gagagcttga tgggtccgac cgcgactcgc gcgcgtccgg
    21841 cgtagagctg gtcgccgagt tcctcggtga gctccgcgcc gggaaggcat cccgccagca
    21901 cgtgcacgtc gtcgagcacc cgccacacct gatcgaacgg cagctccaag gcggtgtcca
    21961 cctgcaccgc gatcgtcgga tctccctcgg ggagcacgat ctccacgttc ccggcaggtt
    22021 cgcccgacgg ttctgcgttc tcctcggtga ccgtggccga ctggccgccg gagcggccga
    22081 ccagcgcccg ccgggcgcag ttgcccggct cggggattcc ctccgggtgc gcggcgcggg
    22141 tttcggagac ggcggtcatg atgttccggt agccggtgca gcggcagatt acgccggaca
    22201 gctccgcggg gagctcctcg tcggtgacct cgggttcgtt gctgagcagg tcgtaggagc
    22261 tgagcaggaa acccggggtg cagaaaccgc attgcagggc gtggttccgc ccgaaggact
    22321 cctgcagcgg gtgcaggtcg tccggacgcc cgagcccttc gacggtgacg acctcggagc
    22381 cgtcgagctg gcaggcgaac agcaggcagg cgcggactgc ttcgccgtcg accagcacgg
    22441 tgcacatccc gcagaccccg tgctcgcagc cgacgtgggt tccggtcagt cccaggtggt
    22501 cgcgcagcgc gtccgccagc gtgacgcggg cggggaggtt cagcaccgcg ggtgtgccgt
    22561 tgaccgtgaa ccgcacttcg acggtttcgc cggcttcggc gcggaccggt cgaaggcctt
    22621 cctgcacggg ttctcgtgcg ctcgtcatgc ttccacctcc gctgtgccgc gcaccgcacg
    22681 gcggtatgcc ttggccgttt cgcgggccgc cagcgcgcgc aggaggcgcc ggcggtagcc
    22741 gttcgacgcg tgcgcgtcgc cgtcggtgtc gacgacctcg cgc
//
