LOCUS       NZ_JADDUE010000008     13503 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Saccharopolyspora sp. HNM0986
            NODE_8_length_361677_cov_70.578231_cov_70.578231, whole genome
            shotgun sequence.
ACCESSION   NZ_JADDUE010000008
VERSION     NZ_JADDUE010000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Saccharopolyspora sp. HNM0986
  ORGANISM  Saccharopolyspora sp. HNM0986
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Saccharopolyspora.
REFERENCE   1  (bases 1 to 361677)
  AUTHORS   Li,F.
  TITLE     Diversity and distribution of actinomycetes associated with coral in
            the coast of Hainan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 361677)
  AUTHORS   Li,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2020) chool of Life and Pharmaceutical Sciences,
            Hainan University, 58 Renmin Avenue, Meilan District, Haikou, Hainan
            570208, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016526145.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 02:15:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,899
            CDSs (total)                      :: 5,838
            Genes (coding)                    :: 5,784
            CDSs (with protein)               :: 5,784
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 7 of 54
            Pseudo Genes (incomplete)         :: 49 of 54
            Pseudo Genes (internal stop)      :: 0 of 54
            Pseudo Genes (multiple problems)  :: 2 of 54
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:09:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 348174
            Orig. end                         :: 361677
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADDUE010000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13503
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="redox-cofactor"
                     /region_number="2"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..13503
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13503
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:12029](-)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12029)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     misc_feature    407..421
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 24.03"
                     /tool="antismash"
     gene            complement(1345..2328)
                     /gene="glpX"
                     /locus_tag="INP57_RS20145"
                     /old_locus_tag="INP57_20140"
     CDS             complement(1345..2328)
                     /EC_number="3.1.3.11"
                     /GO_function="GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /GO_process="GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /gene="glpX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661078.1"
                     /locus_tag="INP57_RS20145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_20140"
                     /product="class II fructose-bisphosphatase"
                     /protein_id="WP_200071824.1"
                     /transl_table=11
                     /translation="MQRQHETAPRTGISTLESVALTATRSAAVASHAWAGRNDKKAADA
                     AATEAMRQVLADAPGRGAVVIGEGEKDEAPMLFNGETVGSGAGPDFDIAVDPLEGTSFC
                     AKNLPGSMATIAFAESGTMFSPGPGFYMDKIVVPPQANGVVDLADTPERTLGKVAGALG
                     KQVGELRVVIMDKPRHQELIKRVLEAGAAVHAPAEGDVGGSLEVLLPSGSADLLLGVGG
                     TPEGVMTAAAVRALGGDMLGRLAPQREAEADALRAAGMDLARIYTRDELVGGEALFAAT
                     GVTGGALLNEPRESGGTVLAESLLISSGEVRRVVHTTFGAMPRPTS"
     gene            complement(2453..2986)
                     /gene="hxlB"
                     /locus_tag="INP57_RS20150"
                     /old_locus_tag="INP57_20145"
     CDS             complement(2453..2986)
                     /EC_number="5.3.1.27"
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /GO_function="GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA]"
                     /GO_process="GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="hxlB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SIS_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661079.1"
                     /locus_tag="INP57_RS20150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_20145"
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="WP_233627905.1"
                     /sec_met_domain="SIS_2 (E-value: 2.5e-10, bitscore: 34.9,
                     seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAISTEVDQIRRGVQPAAWARAGALLLTARAVFTVGTGRSGLALQ
                     MAAMRFMHLGLDTHVVGETTARAIGSGDVLVAASGSGSTARVVRAAETAREQGANVVAL
                     TTDPGSALAKTATETLVIPAADKKDFDGTTSAQYAGSLFEQSVLLLTDALFHALWRSSG
                     TQARELWRLHANLE"
     misc_feature    3203..3218
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.42"
                     /tool="antismash"
     gene            complement(3382..3477)
                     /gene="pqqA"
                     /locus_tag="INP57_RS20155"
                     /old_locus_tag="INP57_20150"
     CDS             complement(3382..3477)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672938.1"
                     /locus_tag="INP57_RS20155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_20150"
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="WP_200071826.1"
                     /transl_table=11
                     /translation="MEPVAPQWETPRFVEIGCAAEVTMYVAQAED"
     misc_feature    complement(3658..3673)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     misc_feature    3934..3944
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            4168..4794
                     /gene="hxlA"
                     /locus_tag="INP57_RS20160"
     CDS             4168..4794
                     /EC_number="4.1.2.43"
                     /GO_function="GO:0043801 - hexulose-6-phosphate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0019647 - formaldehyde assimilation via
                     ribulose monophosphate cycle [Evidence IEA]"
                     /codon_start=1
                     /gene="hxlA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154017.1"
                     /locus_tag="INP57_RS20160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="WP_200071827.1"
                     /transl_table=11
                     /translation="MKLQVALDVATLGDALSLAHQAEDYVDVLELGTPLIKAEGLSAIT
                     AIKAAHPNKLVFADMKTADAGELEADLAFSAGADLVTVMGAADDDTVRGAVAAGEKHGK
                     DVVADMITIVADRVARIREVSKLGVSFVEIHAGLDEQAKPGYTIETLLADGRQAGVPFS
                     IAGGVTAETIAAVQDAGATVAVAGGAIRSAADPAAAAKALKSRIR"
     gene            5032..5763
                     /gene="rpe"
                     /locus_tag="INP57_RS20165"
                     /old_locus_tag="INP57_20160"
     CDS             5032..5763
                     /EC_number="5.1.3.1"
                     /GO_function="GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rpe"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153595.1"
                     /locus_tag="INP57_RS20165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_20160"
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="WP_200071828.1"
                     /transl_table=11
                     /translation="MVDQPGTTSRHSAARWSPAEPVIVGSVLGADYAQLGEEVANLTNA
                     GVGRIQWDVMDRRFVPNMTFGPDVVAACRKYSDLPFEAHLMVQDPDPMLADFVAAGCET
                     VIVHAETCPHLLRTLAAIRELGAQAGVAVNPSTPLGFVRHVLDHLDEIVIMTVNPGFGG
                     QRYIPAMEEKVSEMRALVDGSGRAVQIEVDGGISTATAKAAWCAGAELLVAGSAVLSHP
                     GGKQKAVAELHRAMAPPPPEQ"
     gene            5829..6077
                     /locus_tag="INP57_RS20170"
                     /old_locus_tag="INP57_20165"
     CDS             5829..6077
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983460.1"
                     /locus_tag="INP57_RS20170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_20165"
                     /product="hypothetical protein"
                     /protein_id="WP_200071829.1"
                     /transl_table=11
                     /translation="MALQQAQSSLLLDAAAVLRRRYVRGGRDDSVSPAAAEGLSNFLEG
                     IARSEPALHQADLDEAIALAHRLIDDDHPEHSRLWPA"
     gene            6153..8270
                     /gene="tkt"
                     /locus_tag="INP57_RS20175"
                     /old_locus_tag="INP57_20170"
     CDS             6153..8270
                     /EC_number="2.2.1.1"
                     /GO_function="GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="tkt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1204: transketolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983439.1"
                     /locus_tag="INP57_RS20175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_20170"
                     /product="transketolase"
                     /protein_id="WP_200071830.1"
                     /transl_table=11
                     /translation="MTAIEANRRVRDDITRLTTAAVPADWTDLDVRAIDTARVLAADAV
                     QQCGSGHPGTAMSLAPTAYALFQHVMRHDPNDPSWLGRDRFVLSAGHSSLTLYVQLFLA
                     GYGLAIDDIKALRTWGSRTPGHPEYGHTSGVETTTGPLGQGLANGVGMAMAARRERGLL
                     DPEAAPGESPFDHQIYVIASDGDIEEGVTSEASSLAGTQQLGNLTVIYDANEISIEDDT
                     KIALSEDTAKRYEAYGWHVITVDGGEDVSSILDALDAAKGKPDQPTLIVLRTVIGYPAP
                     NKMNSGKAHGAALGTEELAEVKKLLGFEPERSFQVDDGVLDHTRAVGERGRAARAKWQV
                     AFDAWAEANPRRKALLDRMQARELPPGWAEELPSWEPAEKGVATRKASAEVLAHLADVL
                     PELWGGSADLAESNNTTMKGAESFGPESISTGTWSANPYGRTLHFGIREHAMGSILNGI
                     ALHGGTRPYGGTFLVFSDYMRPAVRLAALMHQPVIYVWTHDSIGLGEDGPTHQPVEHLS
                     SLRAIPGLAIARPADANETAAAWKAALERSDGPTGLALTRQGVPTLEGTDPEGVSRGGY
                     VLAEASSGEPAVLLIATGSEVQLAVAAKKVLESEGEATRVVSMPCVEWFDAQDESYRRA
                     VLPPAVRARVVVEAGIAQCWHRFAGAAGEIVSLERFGASADHQTLFTEFGFTAEAVAEA
                     ARRALRTTRSS"
     gene            8332..8820
                     /locus_tag="INP57_RS20180"
                     /old_locus_tag="INP57_20175"
     CDS             8332..8820
                     /EC_number="5.3.1.6"
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242309.1"
                     /locus_tag="INP57_RS20180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_20175"
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="WP_200071831.1"
                     /transl_table=11
                     /translation="MHVYLGSDHAGFELKNHIAERLAALGHEVTDVGPTAFDAEDDYPP
                     FCVEAARRVVAHQGSLGIVIGGSGNGEQLAANKVPGARAALTWSVQIAELSRKHNDALL
                     AGIGARMHTVEEAERIVDAFLGTAFEGGRHRRRIDQLADYERSGEPPALPGGRPARDH"
     gene            complement(8914..9564)
                     /locus_tag="INP57_RS20185"
                     /old_locus_tag="INP57_20180"
     CDS             complement(8914..9564)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153997.1"
                     /locus_tag="INP57_RS20185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_20180"
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="WP_295146540.1"
                     /transl_table=11
                     /translation="MTTSEAATAAQASHSNEESEPVSGPLSGDPALIGVPTFIVGSIAL
                     GMTLVGFVPAEAVGAPVAIILAATGIGQTVAAIWAAALGQSAVASIFGIFSGFWSSYGL
                     LVLGSTHEWFGVPAGAATATQALFLTSWAITVGLLALASLRLPAAYPLLFGLITTALVV
                     VLIATVQGSAGLQAVGGYVVFTFAALGCYLFLHGMSVATGGRGLPLGGPVLNG"
     gene            complement(10001..11074)
                     /gene="pqqE"
                     /locus_tag="INP57_RS20190"
                     /old_locus_tag="INP57_20185"
     CDS             complement(10001..11074)
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0018149 - peptide cross-linking [Evidence
                     IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079635.1"
                     /locus_tag="INP57_RS20190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_20185"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="WP_200071832.1"
                     /sec_met_domain="PF04055 (E-value: 7.3e-23, bitscore: 76.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 3.7e-08, bitscore: 28.1,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.6e-10, bitscore:
                     35.6, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 1.1e-08, bitscore: 29.9,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDAPYGLLAELTHKCPLHCLYCSNPVALRPRDEELGTEDWLRVL
                     REAAELGVLQVHFSGGEPLLRNDLEELIAEADRCELYTNLITSGLGLTERRAKGLVAAG
                     LDSAQLSIQGDNPESTALIADSKRFDKKAEAAGIIRDSGLPLNMNVVLHRLNLDRLDAI
                     IDVCDSWGAERLELANTQYYGWGLRNREVLMPSREQLTNGEQVYRRRKEELADRMELLW
                     ILPDYYERYPKPCMGGWAHSSFTVAPDGMAYPCPVAADIDSLEFPSVRAAALEWIWTES
                     AAFQAFRGTDWMPDPCRSCARKEIDFGGCRCQAFALTGDAARTDPVCVHSPDHHLISEA
                     VERANGNAPASEPVFRR"
     gene            complement(11071..11334)
                     /gene="pqqD"
                     /locus_tag="INP57_RS20195"
                     /old_locus_tag="INP57_20190"
     CDS             complement(11071..11334)
                     /GO_function="GO:0048038 - quinone binding [Evidence IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PqqD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154001.1"
                     /locus_tag="INP57_RS20195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_20190"
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="WP_200071833.1"
                     /sec_met_domain="PqqD_RRE (E-value: 1e-22, bitscore: 74.1,
                     seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 1.2e-14, bitscore: 48.8,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDPGGKPHLVPKARLRFDKVRGGDMLLLPERVVQLNTSSAAILRL
                     CDGTRTAAEITAKLEADFETDGLSDDVLGFLREAREKGWVKT"
     aSDomain        complement(11077..11319)
                     /aSDomain="PqqD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing protein in a pyrroloquinoline
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_INP57_RS20195_PqqD_RRE.1"
                     /evalue="8.40E-24"
                     /identifier="RREFam006.1"
                     /label="INP57_RS20195"
                     /locus_tag="INP57_RS20195"
                     /protein_end="86"
                     /protein_start="5"
                     /score="74.1"
                     /tool="antismash"
                     /translation="KPHLVPKARLRFDKVRGGDMLLLPERVVQLNTSSAAILRLCDGTR
                     TAAEITAKLEADFETDGLSDDVLGFLREAREKGWVK"
     gene            complement(11334..12029)
                     /gene="pqqC"
                     /locus_tag="INP57_RS20200"
                     /old_locus_tag="INP57_20195"
     CDS             complement(11334..12029)
                     /EC_number="1.3.3.11"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR02111"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983458.1"
                     /locus_tag="INP57_RS20200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_20195"
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="WP_200071834.1"
                     /sec_met_domain="TIGR02111 (E-value: 2e-94, bitscore:
                     309.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPLSAADFEDELREVGERRYHHLHPFNERMHAGDLTEQEFRGWV
                     RNRFYYQLNLPIKDAYVLTKLPGSQHRRRWIKRITDHDGTSGNEGGIERWVRLGEAVGL
                     TREELHDTRTVLPGVRFAVDAYVEFCRDRPWLEAVASALTELFAPDLLSRRITDVREHY
                     PWIAPAGLEYFRTRLTQQPADIAHLLELVRDNATTRAQQEACVQALEFKCDVLWTLLDT
                     VQLTFGKDS"
     gene            complement(12026..12913)
                     /gene="pqqB"
                     /locus_tag="INP57_RS20205"
                     /old_locus_tag="INP57_20200"
     CDS             complement(12026..12913)
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661100.1"
                     /locus_tag="INP57_RS20205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_20200"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="WP_200071835.1"
                     /transl_table=11
                     /translation="MLLRCLGVAAGGGYPQWNCACGGCRAARERPERATTHAGLAVSAT
                     GRRWFLLNATPDVHHQIAADPNLHPGPGPRDTPVSGVLLTDAEFDHTIGLLVLREGADL
                     AVHGTPEVLGALETRFPVRHLLSDYAEIDWRPVEPGEPIELDERLRATAFRTGGKPPRY
                     VGASDRPNWEVGYRLEDTATGGTAVYAPTVPRWDDAFAAQVADADCVFCDGTFHTDDEM
                     SRQGTGRQPGSSMGHMPVDGPDGSARKLAELPGRKIYVHVNNTNPILDEASAQRRRLQR
                     QGIEVGHAGLEVEL"
     gene            complement(12917..12991)
                     /gene="pqqA"
                     /locus_tag="INP57_RS30555"
     CDS             complement(12917..12991)
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqA"
                     /inference="COORDINATES: protein motif:HMM:NF019654.5"
                     /locus_tag="INP57_RS30555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="WP_367269045.1"
                     /transl_table=11
                     /translation="MTWEKPEYTVIDVCAEATGYFHRR"
     misc_feature    12952..12972
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.35"
                     /tool="antismash"
     gene            13091..>13228
                     /locus_tag="INP57_RS30390"
                     /pseudo=""
     CDS             13091..>13228
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024770.5"
                     /locus_tag="INP57_RS30390"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="substrate-binding domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MHLRGRAAGRAGATLLDGPERPTAIFAVNDNTAIGALAAAHRRGL
                     R"
     gene            13229..13357
                     /locus_tag="INP57_RS30395"
     CDS             13229..13357
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="INP57_RS30395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_307850185.1"
                     /transl_table=11
                     /translation="MPFDQIARGALDLLLSSDVAEPRTITTTPALIPRASTTAPAT"
     misc_feature    complement(13379..13392)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.85"
                     /tool="antismash"
     misc_feature    13459..13473
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.99"
                     /tool="antismash"
ORIGIN
        1 gggatcgacc tcgttgcgat ggctgatgat cagcggcatc ggcacgtgca cgaagcggcg
       61 ccgccagcgc accacggcgg tctcggtgcg cccggccatg gccgcgtgca ccgcggcgtg
      121 ggccaggcgc aggcagtaga cgctgtcgta ggcgttggcg gtcacgcttc gcacggcgta
      181 gctcgggtcg atgtagcgca ccgtgggcga caggcccgcc ccgatcaggt gcgccgttat
      241 ggtctcccgc agcagcgcac cgatgtcgcc cagcctggcg ttgccggagg ggtcggtggt
      301 gtcggcgagc agaccgcgct cctgcagcag ctcctggccc gctccctcgg ccactacgac
      361 gaccacgaag cccttctcgc gcacgtgccg ctcgacccgg gccaacaggc cgtccggtcc
      421 ttcgagcgcg aacggcactt cggggatgag cacgacgtcg gcggcgttgc gggccagcga
      481 ggcgtagcag gcgatgaaac cggaatgcct gcccatcagc ttcacgatgc cgatgccgtc
      541 gggactcgcc gcggcctcga tggcgaccga gtcgatgaag ttggccgcct gcgcgaacgc
      601 gctctggaag ccgaaggact ggtcggtgta gggcagatcg ttgtcgatcg tcttggggac
      661 cccgatgacc gcgatgtcca gaccgcgccg gcggatcgcc gcggccaggt tcgtcgcggc
      721 gcgcatcccc ccgtcgccac cgatgacgaa cagcgcgtcc accccgcgca ggaccaggct
      781 gtcgaccatt tcctcgggct cctgcccgcc gcgcgagctg cccaggatgg tgccgccgga
      841 gttgtgtatc tcgcggaccc gatccggtgt cagctcctcg gtgtcgtcgc ggttcgcggc
      901 cgtcagcccg cggagcccgt tgcggaagcc gaggatccgc cgcacgccgt agtgcacggt
      961 gagttcctgg acgatgcccc ggatgacgtc gttgagcccc gggcacagcc cgccgcaggt
     1021 caccacgccc gccgtgacgc gggaggagtc gaagtagatc ttccggcggg gtccggccgc
     1081 ctcgaagctc ggcagctcct ccggcgggag gtcgtgctcg gcgagcatcg aaacggtgtc
     1141 ctcgagcagc acccggtcgc cctccgccac gtagtgcggc gaagtccgct tggccgcgag
     1201 catttcgcag aacggtgagt cgtagcggca ctcaccaagg tggcgaacgc gcaggttgtc
     1261 caagtgcatt ttcaccgaat ctcctaaccg acgacggccg tacgacgagg ccggcagatc
     1321 gatcactggg gcgggcacgt gccgtcaaga cgtcggccgg ggcatggcgc cgaaggtggt
     1381 gtgcacgacg cggcgcacct cgccgctgga gatgagcagg gattccgcga gcaccgtgcc
     1441 cccggattcc cgtggctcgt tcagcagcgc tcctccggtt acgccggtgg ccgcgaacag
     1501 cgcttcgccg ccgacgagct cgtcgcgggt gtagatgcgg gcgaggtcca tgcccgccgc
     1561 gcggagggca tcggcctccg cttcgcgctg cggggccagg cggccgagca tgtcgccgcc
     1621 cagggcgcgc accgccgccg cggtcatcac gccctccggc gtgcccccga cgccgaggag
     1681 caggtcggcg gagccggacg ggagcagcac ctcgaggctg ccgccgacgt cgccttccgc
     1741 cggggcgtgc acggcagcgc ccgcctccag cacccgcttg atcagctcct ggtggcgggg
     1801 cttgtccatg atgaccacgc gcagctcgcc gacctgcttg ccgagcgccc cggcgacctt
     1861 gcccagggtg cgctccgggg tgtcggcgag atcgacgacg ccgttggcct gcggcggcac
     1921 cacgatcttg tccatgtaga agccgggccc cggcgagaac atggtcccgg attcggcgaa
     1981 cgcgatggtg gccatcgagc ccggcaggtt cttcgcgcag aacgacgtgc cctcgagcgg
     2041 gtccacggcg atgtcgaagt ccggacccgc accgctgccc acggtctcgc cgttgaacag
     2101 catcggggcc tcgtccttct cgccttcccc gatgaccacc gcgccccggc ccggagcatc
     2161 ggccagcact tggcgcatgg cctcggtggc cgccgcgtcg gcagccttct tgtcgttgcg
     2221 accggcccag gcgtggctgg ccaccgccgc gctgcgggtg gcggtcaggg ccaccgactc
     2281 cagcgtgctg atgccggtgc gcggtgcggt ctcgtgctgg cgctgcatcg cggactcctt
     2341 gctcgtcgtt gcctcggtac ggaggaggga ccggcgaaaa ccggcgcgcc gggcgatcgg
     2401 acatcgcccg cgcgccacgc ggagcgaatg cgctgccggc ggaatccgcc ggtcactcga
     2461 gattcgcgtg cagccgccac agctcgcggg cctgggtgcc gctggagcgc cacagggcgt
     2521 ggaacagcgc gtcggtcagc agcagcaccg actgctcgaa caggctgccc gcgtactggg
     2581 cggaggtcgt cccgtcgaag tccttcttgt cggccgccgg gatgaccagc gtctcggtcg
     2641 cggtcttggc cagcgccgag ccgggatcgg tcgtcagcgc caccacgttc gcgccctgct
     2701 cgcgcgcggt ctcggccgcc cgcaccaccc gcgccgtcga gcccgagccg gaggcggcca
     2761 ccaggacgtc accggagccg atcgcccgcg cggtggtctc acccacgacg tgggtgtcca
     2821 gcccgaggtg catgaagcgc atcgcggcca tctgcaacgc cagaccgctg cgcccggtgc
     2881 cgacggtgaa caccgcgcgc gccgtgagca gcagtgcacc ggcacgtgcc cacgccgcgg
     2941 gctggacgcc cctgcggatc tggtccacct cggtgctgat ggccatggtc gcgtaattga
     3001 aatgcggcgg attcagcttt tcgccgatat tctcgtgagc ggtgtccgca cccgtcattg
     3061 agcacactcc actggtcgat cggatatatt cgtcggttca gtgcgcgagc gccgagcagc
     3121 ggcgccgggc tgcactttcc tggaccccgc cgggttccgg atcgcgaccc ggacgaatcc
     3181 cgctacgaga gactgcacgg atctgtacgt ctggtcaacg cggccgacac gaagagaagg
     3241 cagactcgca cgaacggttt cggggaccga tcaaacggga accgcaggaa tcccgcgccc
     3301 gttgcggcga caccgcgggc accgggaatc cggtgcccgc ggtcgccgcg ttaccgcccg
     3361 gccgtcgacc ggcgggcggg ctcagtcctc ggcctgggcg acgtacatgg tcacctcggc
     3421 cgcgcacccg atctcgacga accggggcgt ttcccactgc ggggcaacgg gctccatggc
     3481 tacacctcct ttcggaagat ctgcgggcaa ggccctgacc agccccaggg gtttccggaa
     3541 cgatcggtgc cccggcggcg atgcgaacca ccggtaccga ggccgagagc cgctgcgcct
     3601 cccggcgcga tgccgaccgg ctgttcccga aaagaatggg ccgcttcgcg aacagcggtg
     3661 accgccgggt cacgcggctg ggcggggccg aaatcccgaa ctcgccgatc gcgtttcgat
     3721 cgggtcgata ttcgactgct ccacgagggc cggtcccgcc ttccgggaac acccggccga
     3781 tcaggagcgc cagtcatcgt acgtcggcgc tcatccggat cgcaacacca ttctgcggcg
     3841 ttccccgaaa taccatttgg aggttcgtcc gcgccgtccg tgctgccgcg gctgccattc
     3901 gttcggccgc ggttgactag acgtacagat ccgtgcagtg tcacaggccg ttgcccgttc
     3961 gcactccgac gcggaatccg cggagaaccg gaacgagcag ccggaaggat tcgaggaacg
     4021 gcggcacgac gtcgtgatcc acccaccatt ctgcccggaa tcgggaaagc cggatgcggc
     4081 ggaccgcatc gcgcgccgcg tggtggatcc cggcgcagga ccacccgcgc ccgaaaccgc
     4141 aacgccacca ctgacgagga gagcaccgtg aagctgcagg tagcactgga cgtcgccacc
     4201 ctcggcgacg cgctttcgct ggcgcaccag gccgaggact acgtcgacgt cctcgagctg
     4261 ggcaccccgc tgatcaaggc cgaaggcctg tccgcgatca cggcgatcaa ggccgcgcac
     4321 ccgaacaagc tcgttttcgc cgacatgaag accgccgacg ccggcgagct ggaggccgac
     4381 ctcgcgttct ccgcgggcgc ggacctggtg accgtcatgg gcgcggccga cgacgacacc
     4441 gtgcgcgggg ccgtcgccgc cggtgagaag cacggcaagg acgtggtcgc cgacatgatc
     4501 accatcgttg cggaccgggt cgcccggatc cgcgaggtct cgaagctggg cgtgtccttc
     4561 gtcgagatcc acgcggggct cgacgagcag gccaaacccg gctacacgat cgagacgctg
     4621 ctcgcggacg gccgccaggc cggcgtcccg ttctcgatcg ccggtggcgt caccgccgag
     4681 accatcgccg cagtgcagga cgccggggcg accgtcgcgg tggccggcgg tgcgatccgc
     4741 agcgcggcgg acccggccgc agccgccaag gccctcaaga gccggatccg ctgaccggac
     4801 gcagcaagtt cgcgggtggc aggggccgga tcgttcttcg gggcccctgc caccacatca
     4861 ccgctgccac cgcatcaccc ccgccaccac atcaccgctg ccatcgccgc tccggcaggg
     4921 acgaccgaaa accggttcgg gcgatctccg gcgaccgggt ggattcgcct ccaccgatct
     4981 ccgcgggacg cggacacgag tggaccgcac aagtgatcgg aggatcgctc gatggttgat
     5041 cagccaggaa cgacctcgcg gcattccgcg gcgaggtggt cgccggccga accggtgatc
     5101 gtgggttcgg tcctgggcgc cgactacgcg cagctcggcg aggaggtcgc gaacctcacc
     5161 aacgcgggag tcggccgcat ccagtgggac gtcatggacc ggcgcttcgt gcccaacatg
     5221 acgttcgggc cggacgtggt cgcggcctgc cggaagtaca gcgacctgcc gttcgaggcc
     5281 cacctcatgg tgcaggaccc ggacccgatg ctggccgact tcgtcgcggc cggttgcgag
     5341 accgtcatcg tgcacgccga gacctgcccg cacctgctgc ggacgctcgc agcgatccgc
     5401 gagctcgggg cgcaggccgg tgtggcggtc aacccgtcca ccccgctcgg cttcgtccgc
     5461 cacgtgctcg accacctcga cgagatcgtg atcatgaccg tcaacccggg cttcggcggg
     5521 cagcgctaca tccccgcgat ggaggagaag gtctccgaga tgcgggcgct ggtggacggc
     5581 tccgggcgcg cggtgcagat cgaggtggac ggcgggatct ccaccgccac cgccaaggcg
     5641 gcctggtgcg cgggtgccga actcctcgtc gcgggctccg ccgtcctctc ccacccgggc
     5701 gggaagcaga aagcggtggc cgagttgcac cgcgccatgg cgccgccacc gcccgaacag
     5761 tgatcacgag cggcatccgc cgaaggaatt ccgtgcgagg aaggggaaat accgtggagc
     5821 gcaacaagat ggcactgcag caagcgcaat cctccttgct gctggatgca gctgcggtgc
     5881 tgcggcggcg atacgtgcgc ggcggccgcg acgacagcgt gtcccccgcg gcggcggaag
     5941 ggctgtccaa cttcctcgaa ggaatcgccc gctccgaacc cgcgctgcac caggccgacc
     6001 tggacgaggc gatcgccctg gcacaccgcc tgatcgacga cgaccacccc gaacactcgc
     6061 ggctgtggcc cgcctagcgc gccgccgcgt cccacttccg caagttcgca cgaagcaccg
     6121 cgagcaacca cgagcgtcag gagtgcgcgt ccatgaccgc gatcgaagcc aaccgtcgcg
     6181 tgcgcgacga catcacccgc ttgacgaccg ccgccgtccc cgccgactgg accgacctgg
     6241 acgtgcgggc gatcgacacc gcccgcgtgc tggccgccga cgcggtgcag cagtgcggaa
     6301 gcggccaccc gggcaccgcg atgagcctgg cgccgaccgc ttacgcgctg ttccagcacg
     6361 tcatgcggca cgacccgaac gacccgagct ggctcggccg ggaccggttc gtgctctcgg
     6421 cgggccactc gagcctgacc ctgtacgtgc agctgttcct ggccgggtac ggcttggcga
     6481 tcgacgacat caaggcgctg cgcacctggg gttcgcgaac cccagggcac cccgagtacg
     6541 ggcacaccag cggcgtggag accacgaccg gtccgctcgg ccagggcctg gccaacggcg
     6601 tgggcatggc gatggccgcc cgccgcgagc ggggcttgct ggaccccgag gccgcccccg
     6661 gcgaaagccc gttcgaccac cagatctacg tgatcgcctc cgacggcgac atcgaggaag
     6721 gcgtgacctc cgaggcctcc tcgctggccg gcacccagca gctcggcaac ctcacggtga
     6781 tctacgacgc caacgagatc tccatcgagg acgacaccaa gatcgccctg tccgaggaca
     6841 ccgccaagcg ctacgaagcc tacggctggc acgtgatcac cgtcgacggc ggcgaagacg
     6901 tctccagcat cctcgacgcg ctcgacgccg cgaagggcaa accggaccag ccgacgctga
     6961 tcgtgctgcg caccgtcatc ggctaccccg ccccgaacaa gatgaactcc ggcaaggcgc
     7021 acggcgccgc gctgggaacc gaggagctgg ccgaggtcaa gaagctgctg ggcttcgaac
     7081 ccgagcggtc tttccaggtc gacgacgggg tcctggacca cacccgagcc gtcggcgagc
     7141 gcggccgtgc cgcccgcgcg aagtggcagg tcgccttcga cgcgtgggcc gaggccaacc
     7201 cgcggcgcaa ggcgctgctg gaccggatgc aggcgcgcga gctgccgccc ggatgggccg
     7261 aggagctgcc gagctgggag cccgccgaga agggcgtcgc gacccgcaaa gcatcggccg
     7321 aggtcctggc gcacctcgcc gacgtgctgc cggagttgtg gggcggctcc gcagacctcg
     7381 cggagagcaa caacaccacg atgaaaggcg cggaatcgtt tggcccggag agcatctcca
     7441 ccggcacctg gagcgccaac ccctacggcc ggacgctgca cttcggcatc cgcgagcacg
     7501 cgatgggctc gatcctcaac ggcatcgcgc tgcacggcgg cacccgcccg tacggcggca
     7561 cgttccttgt cttcagcgac tacatgcggc ccgcggtgcg gctggcggcg ctgatgcacc
     7621 agccggtgat ctacgtctgg acgcacgact cgatcggcct gggcgaggac ggcccgacgc
     7681 accagccggt cgagcacctg tccagcctgc gtgcgatccc gggcctggcg atcgcgcggc
     7741 ctgccgacgc caacgagacc gccgcggcgt ggaaggccgc gctggagcgt tccgacggcc
     7801 cgaccgggct ggcgctgacc cgccagggag tgccgaccct cgagggcacg gacccggaag
     7861 gggtgtcccg cggcggctac gtgctcgccg aagcttcgtc cggcgaaccc gcggtgctgc
     7921 tgatcgcgac cgggtcggag gtgcagctgg ccgtggctgc caagaaagtc ctcgaatccg
     7981 agggcgaggc gacccgcgtg gtgtccatgc cgtgcgtgga gtggttcgac gcgcaggacg
     8041 agtcctaccg ccgcgcggtg ctgcccccgg cggtgcgcgc gcgggtcgtc gtcgaggccg
     8101 ggatcgcgca gtgctggcac cgcttcgccg gtgcggcggg agagatcgtc tcgctggagc
     8161 gcttcggcgc ctcggccgat caccagacgc tgttcaccga gttcggcttc accgccgagg
     8221 cggtcgccga ggccgctcgg cgcgcgctgc gcaccactcg gtcgagctga cgcccgggcc
     8281 ccgcacgagc ggggccacca cagtcgtcca acacagcgga ggatcgattc cgtgcacgtc
     8341 tacctaggct ccgatcacgc cgggttcgaa ctcaagaacc acatcgccga acgattggcc
     8401 gcactcggcc acgaggtcac cgacgtcgga ccgaccgcct tcgacgccga ggacgactac
     8461 ccgccgttct gcgtcgaggc ggcgcggcgg gtggtcgccc accagggcag cctcggcatc
     8521 gtcatcggcg gttccggcaa cggcgagcag ctcgccgcga acaaggttcc cggggcccgg
     8581 gccgcgctga cctggagcgt gcagatcgcc gagttgtccc gcaagcacaa cgacgcgctg
     8641 ctggcgggca tcggtgcccg gatgcacacg gtcgaggagg ccgagcgcat cgtggacgcg
     8701 ttcttgggca ccgcgttcga gggcggccgc caccggcgca ggatcgacca gctggcggac
     8761 tacgagcgtt ccggcgagcc acctgcgctg ccgggcggcc gaccggcccg cgaccactga
     8821 ggaatccgcg gcggaagttc gaccgacgcg caggagcgcg gaggcggtgc gagaccgcct
     8881 ccgcgcttct cgggtttctc gcgcttccct ggctcagccg ttcaggaccg gaccgcccag
     8941 cggcagcccg cgcccgccgg tcgccaccga catcccgtgg aggaacaggt agcagcccag
     9001 cgcggcgaag gtgaagacca cgtacccgcc gacggcctgc aatcccgcgg acccctgcac
     9061 cgtcgcgatc agcacgacga cgagcgccgt ggtgatcagc ccgaacagca agggatacgc
     9121 ggcgggcagg cgcagcgacg ccagcgccag gagccccacc gtgatcgccc aagaggtcaa
     9181 gaacagcgcc tgggtggcgg tcgcggctcc agctgggact ccgaaccatt cgtgggtcga
     9241 gccgagcacg agaaggccgt agctcgacca gaacccgctg aagatgccga aaatggacgc
     9301 gaccgcgctc tggccgagcg cggccgccca aatcgcggcc acggtctggc cgattccggt
     9361 ggcggccagg atgatcgcca ccggggcgcc gaccgcctcg gcgggcacga atccgacgag
     9421 ggtcatgccg agggcgatcg aaccgacgat gaacgtggga acgccgatga gcgcggggtc
     9481 accggacaac ggcccggaaa ccggctcgga ttcctcgttc gagtggctgg cttgcgctgc
     9541 cgtggcagct tccgatgtgg tcacaatccg ctccttcgcg gtgttcggcg catgcttcgc
     9601 gccgtccgga tcgaatccgg cgccatgccg gatttcgagt ggacgacggt tttccgggcg
     9661 gagtcgccgg tgcggattcc gaaggtttcc gagcgggcgc ggcacattga gaatgccact
     9721 ggcccgtgcc gaggacaacg agttgcgaac gagtggaagc cgattcccca cgaagggccc
     9781 gacccagctg ccgaacggct gcaccgggca ccttcccggt tctagccgcg gtccgcgacg
     9841 aagtccgtcc tttccaacga gatgtccgcg tgtttcgacg agaagtcctc gcgcatgccg
     9901 cgcagaacca cttccgggag ctgaagaatt cggcccggct tccggggcag cggttgcacg
     9961 cctgaacgct gccgggaagc gcagttcacg cgaacacgca ctacctccgg aaaaccggtt
    10021 ccgatgccgg agcgttgccg ttggcgcgct ccacggcctc ggagatcagg tggtggtccg
    10081 gcgagtgcac gcacaccgga tcggtgcgcg cggcgtcccc ggtgagcgcg aacgcctggc
    10141 agcggcagcc cccgaagtcg atttccttgc gcgcgcagct gcggcacgga tcgggcatcc
    10201 agtcggtgcc ccggaacgct tggaacgcgg ccgattccgt ccagatccat tccagcgcgg
    10261 cggctcgcac cgaggggaat tcgaggctgt cgatgtcggc ggccaccggg cacggatacg
    10321 ccatcccgtc cggggcgacg gtgaagctgg aatgcgccca cccgcccatg cacggcttcg
    10381 ggtaccgctc gtagtagtcc ggcaggatcc acagcagctc catgcggtcg gccagctcct
    10441 ccttgcggcg ccggtagacc tgctcgccgt tggtcagctg ctcccggctg ggcatcagca
    10501 cctcgcggtt gcgcagcccc cagccgtagt actgggtgtt ggccagttcg aggcgctcgg
    10561 caccccatga gtcgcagacg tcgatgatcg catccaggcg gtcgaggttg agccggtgca
    10621 gcacgacgtt catgttcagc ggcagcccgc tgtcgcggat gatgcccgcc gcctccgcct
    10681 tcttgtcgaa ccgcttgctg tccgcgatca gcgcggtgga ctccgggttg tcgccctgga
    10741 tgctcagttg cgcgctgtcg aggccggcgg cgaccaggcc cttcgcccgg cgctcggtga
    10801 gcccgagtcc gctggtgatc aggttggtgt agagctcgca gcggtccgcc tcggcgatca
    10861 gctcctccag gtcgttgcgc agcagcggct cgccgccgga gaagtgcacc tgcagcacgc
    10921 cgagctcggc ggcttcccgg agcacccgca gccagtcctc ggtgcccagt tcttcatcgc
    10981 gcggccgcag cgccaccggg ttcgagcagt acaggcagtg cagcgggcac ttgtgggtaa
    11041 gctcggccag caagccgtag ggcgcatcga tcatgtcttc acccagccct tttcgcgcgc
    11101 ttcgcgcagg aagcccagca cgtcgtcgga tagcccgtca gtctcgaagt cggcttccag
    11161 cttcgcggtg atctcggcgg cggtgcgggt gccgtcgcac agccgcagga tcgcggcgct
    11221 ggaggtgttg agctgcacca cccgctcggg cagcagcagc atgtccccgc cgcggacctt
    11281 gtcgaagcgc agcctggcct tcggcaccag gtgcggcttg ccgccggggt ccatcacgag
    11341 tccttgccga acgtcagctg caccgtgtcc agcagtgtcc acagcacgtc gcacttgaac
    11401 tccagcgcct gcacgcacgc ctcctgctgc gcgcgcgtgg tcgcgttgtc gcgcaccagt
    11461 tccagcagat gcgcgatgtc ggcgggctgc tgggtcaacc gggtgcggaa gtactccagg
    11521 cccgcggggg cgatccacgg gtagtgctcg cgcacgtcgg tgatgcggcg gctgagcagg
    11581 tccggggcga acagctcggt cagcgccgag gcgaccgcct ccagccacgg ccggtcgcgg
    11641 cagaactcca cgtaggcgtc caccgcgaag cgcaccccgg gcagcaccgt gcgggtgtcg
    11701 tgcagctcct cgcgggtgag cccgaccgcc tcgccgagcc gcacccagcg ctcgatgccg
    11761 ccctcgttgc ccgatgtgcc gtcgtggtcg gtgatccgct tgatccaccg cctgcgatgc
    11821 tgcgagcccg gcagcttggt gaggacgtag gcatccttga tcggcaggtt gagctggtag
    11881 tagaagcggt tgcgcaccca gccgcggaac tcctgctcgg tcaggtcgcc ggcgtgcatc
    11941 cgctcgttga acgggtgcag gtggtggtag cgccgctcgc cgacctcccg cagctcgtcc
    12001 tcgaagtccg cagcggacaa cggcgtcata gctcgacctc caaaccggcg tgcccgacct
    12061 cgatgccctg ccgctgcaac ctgcggcgtt gcgcggatgc ctcgtcgagg atcgggttgg
    12121 tgttgttgac gtggacgtag atcttgcggc cgggcagctc ggcgagcttc cgcgcggacc
    12181 cgtcgggacc gtccaccggc atgtgcccca tggacgatcc cggctgccga ccggtgccct
    12241 ggcgcgacat ctcgtcgtcg gtgtggaagg tgccgtcgca gaacacgcag tcggcgtcgg
    12301 cgacctgcgc ggcgaatgcg tcgtcccagc ggggcaccgt cggggcgtac accgccgtgc
    12361 cgccggtcgc ggtgtcctcc agccggtagc ccacctccca gttcgggcgg tccgaggcgc
    12421 ccacgtagcg cggcggtttg ccaccggtgc ggaacgcggt ggcgcgcagg cgctcgtcca
    12481 gctcgatcgg ctcgcccggt tccaccggcc gccagtcgat ctcggcgtag tcgctgagca
    12541 ggtgccgcac cgggaaccgc gtttcgagcg ctccgagcac ctcgggcgtg ccgtgcacgg
    12601 cgaggtccgc gccctcgcgc agcacgagca ggccgatggt gtggtcgaat tcggcgtcgg
    12661 tgagcagcac cccggacacc ggggtgtccc gcggcccggg tccgggatgc aggttggggt
    12721 cggcggcgat ctggtggtgc acgtcggggg tggcgttgag caggaaccat cgcctccccg
    12781 tggcggagac cgcgagacct gcgtgcgtcg tggcccgttc gggccgttcg cgggccgcgc
    12841 ggcagccgcc gcaggcgcag ttccactggg ggtaaccgcc gcctgcggcg actcctagac
    12901 aacgcagcag cacggctcag cgccggtgga agtagccggt cgcctcggcg cagacatcga
    12961 tcacggtgta ttcgggcttt tcccaggtca tgccatcctc ctagaacaac gggacggctt
    13021 cgagcctccg cgatcggtgg ttgcggtgac aacacatcgg caaccagcgg aacccgatcc
    13081 ccgatcagag gtgcatttgc ggggacgagc ggctgggcgt gcaggcgcca cacttctcga
    13141 cggccccgaa cgccccaccg cgatcttcgc tgtcaacgac aacaccgcca tcggcgccct
    13201 cgccgccgcg caccgccggg gacttcgcgt gccattcgac cagatcgctc gcggcgcgct
    13261 ggacctgctg ctgagctcgg acgtcgccga accccgcacc atcaccacca caccggcgct
    13321 gatcccgcgc gcttcgacca ccgcacccgc gacctgactc gccgtcacgt gcaatggctg
    13381 catgctcacc cagccgggca agctggtcac gcacccgccg ggtgcccaac cggtgacgat
    13441 ggtgctgcat gatggaacgg atcttttcgg tcaaggtgcc gtcacggcgt tgttgctgtg
    13501 tgg
//
