LOCUS       NZ_JADDUE010000002     10813 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Saccharopolyspora sp. HNM0986
            NODE_2_length_718680_cov_78.684148_cov_78.684148, whole genome
            shotgun sequence.
ACCESSION   NZ_JADDUE010000002
VERSION     NZ_JADDUE010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Saccharopolyspora sp. HNM0986
  ORGANISM  Saccharopolyspora sp. HNM0986
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Saccharopolyspora.
REFERENCE   1  (bases 1 to 718680)
  AUTHORS   Li,F.
  TITLE     Diversity and distribution of actinomycetes associated with coral in
            the coast of Hainan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 718680)
  AUTHORS   Li,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2020) chool of Life and Pharmaceutical Sciences,
            Hainan University, 58 Renmin Avenue, Meilan District, Haikou, Hainan
            570208, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016526145.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 02:15:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,899
            CDSs (total)                      :: 5,838
            Genes (coding)                    :: 5,784
            CDSs (with protein)               :: 5,784
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 7 of 54
            Pseudo Genes (incomplete)         :: 49 of 54
            Pseudo Genes (internal stop)      :: 0 of 54
            Pseudo Genes (multiple problems)  :: 2 of 54
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:09:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 287764
            Orig. end                         :: 298577
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADDUE010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10813
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10813
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10813
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5813](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5813
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            119..472
                     /locus_tag="INP57_RS05660"
                     /old_locus_tag="INP57_05660"
     CDS             119..472
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF023857.5"
                     /locus_tag="INP57_RS05660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_05660"
                     /product="CopG family antitoxin"
                     /protein_id="WP_200068972.1"
                     /transl_table=11
                     /translation="MTATKKKDQGQARSTEELDELAAYYDAHDTSEEMEQGQWVEPQPM
                     KTTSLRLPADVVEALKKLAQARGVRYTALLREIVEQAVYETHSVHSDELAEINQRLARI
                     EAELDSKPATTER"
     tRNA            583..654
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="INP57_RS05665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="INP57_05665"
                     /product="tRNA-OTHER"
     gene            583..654
                     /locus_tag="INP57_RS05665"
                     /old_locus_tag="INP57_05665"
     gene            731..967
                     /locus_tag="INP57_RS05670"
                     /old_locus_tag="INP57_05670"
     CDS             731..967
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="INP57_RS05670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="INP57_05670"
                     /product="hypothetical protein"
                     /protein_id="WP_200068973.1"
                     /transl_table=11
                     /translation="MMGLARKPAARPLSVVLSGVAVFVAGLVWSLSVHTPGTGVHFGAA
                     TLITMGELAILIGIVIGIRAFITSRRRKDTDDP"
     misc_feature    821..823
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1164..1862
                     /locus_tag="INP57_RS05675"
                     /old_locus_tag="INP57_05675"
     CDS             1164..1862
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494880.1"
                     /locus_tag="INP57_RS05675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_05675"
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_233626950.1"
                     /transl_table=11
                     /translation="MMSPLAPGRYLDSIAVDGAEIGRLCRLHPAQTVPSCPDWTGADLL
                     AHLTGFTRWLRGLYDGKGDIAAPVPVIDPGACTLEWDRTLEELLSLLRGTDPQAAVPNW
                     SIGPQTAKFWLRRCAQDVAIHRWDAARLISRSPDPVPADIALDGVDEYLHVFIGTALAG
                     GATPEAEATIVLEMTDLDRHVRRDLPRPGPVTTLRGAASDLLLGLWHRRDPLGLFVSGD
                     RELVAAWPRI"
     misc_feature    complement(2001..2012)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     gene            2119..2319
                     /locus_tag="INP57_RS05680"
                     /old_locus_tag="INP57_05680"
     CDS             2119..2319
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="INP57_RS05680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="INP57_05680"
                     /product="hypothetical protein"
                     /protein_id="WP_200068974.1"
                     /transl_table=11
                     /translation="MSHPVKQRIAVGVLLVLCGGVVALAALNLFDLIRTPNLVVAGAAI
                     VVSLVVLGLVMGWVPTRRRRN"
     gene            2692..3984
                     /locus_tag="INP57_RS05685"
                     /old_locus_tag="INP57_05685"
     CDS             2692..3984
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816456.1"
                     /locus_tag="INP57_RS05685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_05685"
                     /product="MFS transporter"
                     /protein_id="WP_200068975.1"
                     /transl_table=11
                     /translation="MPIALLALALGAFGIGTTEFVMMGVLPQAAADLGVSISTAGNLIT
                     GYALGVVIGAPTLTLLGNRLPRKTVLMGTMGLFTAGNALFALAPSAGVGMAFRFVAGLP
                     HGAFFGVGAVVAAGLVRKADRAKAVSMMFMGLTLANVVGVPLGTLLGQQVGWRATFAVV
                     AAVGVLAIAAIARLLPAMGRPGPEETSVRGQLAAFRKPQVWLALAIVTFGLGGTFACLS
                     YLTPILTGVSGYSETGATVLLMVAGLGMTVGNVLGGRVADKALVPGLYAALIGVAAVLG
                     AFTVTSGNQVAAAVTVFLIGVGGFMVAPIMQAMIMEKAGGTPLLVSAAVQSAFNIANSI
                     GACLGGLAIAHGLGLTGPSMVGSALTMLGLGLAVVSASVDRKVQRKVPARSASDESVSE
                     EAASEETVAEGSGTDELAPARPQREPELAAV"
     gene            4147..5004
                     /locus_tag="INP57_RS05690"
                     /old_locus_tag="INP57_05690"
     CDS             4147..5004
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="INP57_RS05690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_05690"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_200068976.1"
                     /transl_table=11
                     /translation="MDSPWGLNGPDYLLAYALVLAMLCVCLFAVRAQAKEKQVWAGEKT
                     DPGLYARAYLNGASGRVADTALAGLIELGAVRVNRRRELYSTGRRPADDVQRQLLEGLG
                     DRSTVSRVRAQFKESEYCDSLLHLLREDRLIVSARHRTMLLVLLWGFPVLMLINVVRAV
                     NGAELGFPIGGLIIELAITLFVWLFAAGKCGHPMPTDAGDAVVRPSTGKHAESGTQDHA
                     SRLPEDDAANQVAAYGLSAYPDRTIAELLEPPNSARGGRAGGVGGGGGCGGGGGGGCGG
                     GGGG"
     gene            5001..5813
                     /locus_tag="INP57_RS05695"
                     /old_locus_tag="INP57_05695"
     CDS             5001..5813
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658035.1"
                     /locus_tag="INP57_RS05695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_05695"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_233626951.1"
                     /sec_met_domain="DUF692 (E-value: 1.3e-72, bitscore: 239.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTASESPVLGVGIGWRAEIDLTVERLRTDFVEFIAEDVDPRRIPE
                     SLRLLLQRGTPALPHAVSLSLAGAEPLRTERVRHLARVAAAVDAPLVSDHVAFTRAGEW
                     EAGHILPPPRTRESLDVLVDNVRRAQDELPVPLALEHVAALVEWPGAEFGEAEFLARLV
                     ERTGCLLIVDVANLYANAHNLGTDPAAFLDTVPLERVAYVHVGGGVWRDGTYHDTHAHP
                     VLPEVLELLCELGARTRPRGVLLERDDRFPDDDELAAELAAIRRAVAR"
     gene            5810..6133
                     /locus_tag="INP57_RS05700"
                     /old_locus_tag="INP57_05700"
     CDS             5810..6133
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684641.1"
                     /locus_tag="INP57_RS05700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_05700"
                     /product="hypothetical protein"
                     /protein_id="WP_200068977.1"
                     /transl_table=11
                     /translation="MSRERLAAQQAELLGALLGQRQDPPGFDRAQLRVQQQALLDKRRR
                     VVARLRPDLPELLGDRFRSLFERYAAEQPRAAGSKARADAAEFAGWLSRRGELRRRRWL
                     PRR"
     gene            complement(6201..6671)
                     /locus_tag="INP57_RS29360"
     CDS             complement(6201..6671)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="INP57_RS29360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_233626952.1"
                     /transl_table=11
                     /translation="MNHPQRGPNQYPQYGNPPPPGHGPHYGYDQNPYPPHQYDHGAPPD
                     GYYDRPPEGRSRDGRGDRGDSGANTLANIVDVLTSVIVLAFVLHILFVVFDANQGNQFV
                     SAVYTLAKTLVLGLGDVFTPNDALLGVVLNYGVAALVYLVIGRLIARALRRR"
     gene            7085..8011
                     /locus_tag="INP57_RS05710"
                     /old_locus_tag="INP57_05710"
     CDS             7085..8011
                     /GO_process="GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875006.1"
                     /locus_tag="INP57_RS05710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_05710"
                     /product="LCP family protein"
                     /protein_id="WP_295149588.1"
                     /transl_table=11
                     /translation="MLLLALAGFGLYVDMSLKREDALPAGGERPAAGPGTNWLLVGSDS
                     REGLDAERQAELGTGDAAGRRTDTMMLVHIPESGGEPTMVSLPRDSYVRIPGKGKDKLN
                     AAFAYGGPELLSRTVEGATGVRIDHYAEIGLGGFADITDAVGGVDLCVKERMTDPKASL
                     DLQPGCQTVNGPQALGYVRSRASSRGDLDRVEHQREFLAALTERASSPQVLLNPFRLFP
                     LITDTSGAFLVGRDEHVWHLGALAFAMNGIASGKGITTTVPMSGFGESSDGASVIKWDA
                     EKAAQLFGALAKDEPVPAGAAGAGFGG"
     gene            complement(8050..8655)
                     /locus_tag="INP57_RS05715"
                     /old_locus_tag="INP57_05715"
     CDS             complement(8050..8655)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.5"
                     /locus_tag="INP57_RS05715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_05715"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_200068979.1"
                     /transl_table=11
                     /translation="MARTTGSSAARTRARVLQAARELFTERSYAGTSIRDIAERLEMTK
                     AALYYHFPAKEDLLRELVAPVVDELGACVRAAEDGSESSRRLLRRVVDLFDDHRHIIRG
                     TLYDASARSVLLAKDDMFADVRALENALAASDDPADLLLARCAIGTVRGAIISTGDIDS
                     LALNRPPEADVGPRLSEQDRELVTNAAVAVLDTGARDD"
     misc_feature    complement(8754..8769)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.84"
                     /tool="antismash"
     gene            8754..9470
                     /locus_tag="INP57_RS05720"
                     /old_locus_tag="INP57_05720"
     CDS             8754..9470
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674243.1"
                     /locus_tag="INP57_RS05720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_05720"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_200068980.1"
                     /transl_table=11
                     /translation="MTNRSGAGIDIGSVDFESFYRGEGGFADIEFDRAPWDIGEPQPVL
                     VQLERDGGITGRVLDAGCGPGDNAIFLAERGYSVTGVDGSATALGTARERAAARGVRAD
                     FQVADATTMEGVPQEFDTVLDSALYHCLPAESRAAYGAALHRVTKPGAKLHVLCFADVE
                     DAPAPATVSQDDLRTNLGEHWRINAIEQVRYSGAFDPETAARHFEQFMPFDAGAAITDD
                     NGRFTFPMWHLQAERA"
     misc_feature    complement(9603..9623)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.33"
                     /tool="antismash"
ORIGIN
        1 gctacctgtt cgtggtggta gcgcctgcca acggccaccc tggtgcggct tacatcgtga
       61 ccgcacgcga tatgacgccg agcgagaagc aaacgttcct gcggaaggcg cgataactat
      121 gacggcaacg aagaaaaagg atcaaggtca agcgaggtcg accgaagagc tcgatgagct
      181 ggccgcctac tacgacgcac atgacacctc cgaagagatg gaacaagggc agtgggtcga
      241 accacagccg atgaagacga catctctgcg gcttccggcc gatgtggtcg aggcgttgaa
      301 gaagctcgct caagcacggg gggtccggta caccgctctg ctgcgagaga tcgttgagca
      361 ggctgtgtat gaaacccact cggtgcacag cgacgagcta gcagagatta accagcgcct
      421 cgctcgcatc gaggccgaac tcgactcgaa acccgctacg accgagcgct gacccgatca
      481 accaccgacg gctcgcgggc acccggccgt gggccgaccg ccgggcggag tcgtctggcc
      541 gatcaccggc catggcaacc tgtgctcggc cttgactggc aggccaggtg tggtcaggga
      601 gctgactgta aatcagccgc gcaagcttca aaggttcgaa tcctctaccc ggcacaccag
      661 ccacgacgcc ccggtgacct gccgaaacgg tcaccgggtc attgtgcgct gtccgacaag
      721 gaaatccgct gtgatgggtc tcgcgcgtaa acccgctgca cgtccgttga gcgtcgtcct
      781 gtccggtgtg gccgtgttcg tcgcgggact cgtgtggtcc ttatcggtcc acactcccgg
      841 cacgggggtt cacttcggag ccgcaacact gatcacgatg ggcgaactcg ccatcctcat
      901 cgggatcgtg atcggaatca gagcgttcat cacgtcacgc cgccgcaagg acaccgacga
      961 cccctgactc cttcgccggg agttctggtg gcacggggtc cgatcagcag gctgcgggag
     1021 gctcctgcac cgctctcgcg aacggattac tcgctgatta gcgagctctc gcaccccgag
     1081 gcggggagca tggtcgcggt gagatgccgg gaggtcggcg aaatcgggcg ccgcctcggc
     1141 gtcgagttga aacactcgac gccatgatgt cgccgctggc ccccggtcgt tacctcgaca
     1201 gcatcgccgt ggacggcgcc gagatcggcc ggctgtgccg gttgcaccct gcgcagaccg
     1261 tgccgagctg tccggactgg accggcgcgg atctgctcgc gcacctgacc ggtttcaccc
     1321 gctggctgcg cggcttgtac gacgggaagg gcgacatcgc ggctccggtt cccgttatcg
     1381 atcccggggc ttgcacgctg gagtgggatc ggactctcga ggagctgctc tcgctgctgc
     1441 gcgggaccga tccgcaggca gcggtgccga actggtcgat cgggccgcag accgcgaagt
     1501 tttggctgcg ccgctgcgcg caggacgtgg cgattcaccg ctgggacgcg gcgcggctga
     1561 tcagtcgcag tccggacccg gtgcctgccg acatcgcgct cgacggcgtc gatgagtacc
     1621 tgcacgtgtt catcggcacc gcgctcgccg gtggcgcgac acccgaggcg gaggccacga
     1681 tcgtcctcga aatgaccgat ctcgaccggc acgtccgccg cgatctgccg cgcccgggcc
     1741 cggtgaccac cctgcgcggc gctgcttccg acctgctgct cggactgtgg caccggcgtg
     1801 acccgctggg cctgttcgtc agcggcgacc gggagctcgt cgccgcgtgg ccgcgcattt
     1861 gattcggacg ggcgccgtca gcttggactc gtatcttgcc ggcaaggtct cgacctgcgc
     1921 cgcgggtgaa tcggtcattg tggccggtgt cggtgcagct ggggaacagc gcgcctgccg
     1981 gcgccatttc agcagtgctc gcgttccttt ccgccgtggt gaacgtgtgc aagcacgaga
     2041 acggctcgag ctacgtgtac ttgatcggtg cgccgccgtt cacgccggtg gtctgcaatc
     2101 cgttcgggca acggcgctat gagtcatccg gtcaaacaac ggatcgcggt cggcgtgctg
     2161 ctcgtgctct gcggcggtgt tgtggcgttg gctgcgctga acctgttcga cttgatcaga
     2221 acaccgaact tggtcgttgc gggcgcggca atcgtcgtga gcctcgtggt gctgggcctg
     2281 gtcatgggat gggtgccaac tcggcgacgc cgcaattgat cagcaccccc gagacgtcgg
     2341 ctagaactag ctcagcgaag ctgtgcattg tctgattcgt cggtgttcgg ggcggtgcag
     2401 cgctgggtgc gctgcagtcg gtcacggggt gccatcactc gcgtgacctg cgagaggact
     2461 ccgcaggttg gcccaacgag tgggtgacaa ccggacgccg ccgttgctac gcccggtggt
     2521 gaggttcacc gccagctggg tggacacgtc ctcggcggag ctcggagact gggaaccaag
     2581 acagccgaca gcgacctcca ccgcgaccga ctgtctcttc ttttgagcaa agcctgtatc
     2641 gattcagcaa tggcgctgcc tcatcactcc gcttcccccg agaggagaac tgtgcccata
     2701 gcgctgctcg cactcgccct cggcgcattc ggtatcggca cgaccgaatt cgtcatgatg
     2761 ggcgtgctgc cgcaggccgc cgccgacctc ggcgtttcga tatccactgc cgggaacctg
     2821 atcaccggct acgcgctggg ggtcgtgatc ggggcgccga ccctcacgct gctgggcaac
     2881 cggctaccca ggaagaccgt cctgatggga accatggggc tgttcaccgc gggcaatgcg
     2941 ctgttcgcgc tggcgccgag cgccggagtg ggcatggcgt tccgcttcgt ggccgggctg
     3001 ccgcacggcg ccttcttcgg cgtcggggcc gtggtcgcag ccggcctcgt gcgcaaggcc
     3061 gaccgggcca aggccgtctc gatgatgttc atgggcctga cgctggccaa cgtcgtcggc
     3121 gtcccgctgg gcacgctgct gggccagcag gtcggctggc gcgccacgtt cgccgtcgtc
     3181 gccgcggtcg gcgtcctcgc catcgccgcc atcgcccgct tgctgcccgc catgggcagg
     3241 ccggggccgg aggagacctc ggtgcgcggc cagctcgccg ccttccgcaa gccgcaggtg
     3301 tggctggcgc tggcgatcgt caccttcggt ctgggcggca cgttcgcgtg cctgagctac
     3361 ctgacgccca tcctcaccgg cgtgtccggt tactccgaga ccggcgcgac ggtgctgctg
     3421 atggtggccg ggctcggcat gacggtcggc aacgtcctcg gcggccgggt ggccgacaag
     3481 gcgctggtgc ccggcctcta cgccgcgctg atcggcgtcg cggcggtgct cggtgcgttc
     3541 acggtcacat cgggcaacca ggtggccgcc gcggtgacgg tcttcctgat cggtgtcggc
     3601 ggcttcatgg tcgcgccgat catgcaagcg atgatcatgg agaaggccgg tggaacgccg
     3661 ctgctggtct cggcggccgt gcagtccgcc ttcaacatcg cgaactccat cggcgcctgc
     3721 ctgggcggtc tcgccatcgc ccacgggctc gggctgaccg gtcccagcat ggtcgggtcc
     3781 gcgttgacca tgctcgggct ggggctggcc gtggtgtcgg cgagcgtgga ccgcaaggtt
     3841 cagcgcaagg tccccgcccg gtcggcgtcc gacgagtcgg tgtccgagga ggccgcgtcc
     3901 gaggagacgg tggccgaggg gtccgggacc gacgagctcg cgccggcccg gccgcagcgc
     3961 gaaccggagc tggccgcggt ctgaccggcg aattcacgaa tggcgggcgg ctgcggtcgc
     4021 ccgccattcg gctgttcgcg cccggtgcag cccgtttccg tttccccgga tcagccctct
     4081 ctgcgatcgg cttggttgtt ctaaagtgcg gtgcagctcg ggggatgcca tgtcgagggg
     4141 gaacgcgtgg acagtccgtg gggactgaac ggcccggatt acctgctggc gtacgcgctg
     4201 gtcctggcca tgttgtgcgt atgcctgttc gcggttcgcg cgcaggcgaa ggagaagcag
     4261 gtctgggcgg gcgagaaaac ggatcccggt ctctacgcca gggcatacct caacggcgcg
     4321 tcgggacggg tggccgacac cgcgttggcg ggattgatcg aactcggggc ggttcgggtg
     4381 aaccggcgcc gcgagctcta cagcaccggc cgcaggcccg ccgacgacgt gcagcgccaa
     4441 ctgctggagg ggctcggtga ccgcagcacc gtcagccgag ttcgcgcgca gttcaaggag
     4501 agcgagtact gcgactcgct gctgcacctg ctgcgcgaag accgcctgat cgtctccgcc
     4561 cgccatcgca cgatgttgct ggtcctgctg tggggctttc cggtgttgat gctgatcaac
     4621 gtggtgcggg cggtcaacgg ggcagagctc ggcttcccga tcggcggcct gatcatcgag
     4681 ttggcgatca cgcttttcgt gtggctgttc gcggccggga aatgcgggca cccgatgccc
     4741 acggacgccg gggacgccgt tgtgcggccg agcacgggca agcacgccga gagcggcacg
     4801 caggaccacg cttcccggtt gccggaggat gacgccgcca accaggtcgc cgcgtacggc
     4861 ctgagcgcct acccggaccg gacgatcgcg gagctgctgg agccgccgaa ctccgctcgc
     4921 ggcggacgag cgggcggcgt cggtggtggc ggtggctgcg gcggtggcgg tggtggtggc
     4981 tgcggcggag gaggcggcgg ttgaccgcct cggaatcacc ggtgctgggt gtcgggatcg
     5041 gctggcgggc cgaaatcgat ctcaccgtgg aacggctgcg gaccgatttc gtcgagttca
     5101 tcgccgagga cgtcgacccg cgccgcatcc cggagtcgct gcgcctgctg ctgcaacggg
     5161 gaacgcccgc cttgccgcac gccgtgtcgc tgtcgctggc cggagcggaa ccgctgcgga
     5221 ccgagcgggt gcggcacctg gcgcgagtgg ccgcggcggt cgacgcgccg ctggtcagcg
     5281 accacgtcgc attcacccgc gccggcgagt gggaagccgg gcacatcctc ccgccaccgc
     5341 gcacccgcga atcgctggac gtgctggtcg acaacgtgcg gcgcgcccag gacgagctcc
     5401 cggtcccgct ggccctggag cacgtggccg cgctggtgga atggccgggt gccgagttcg
     5461 gcgaagcgga gttcctcgcc aggctcgtcg agcgcaccgg ctgcctgctg atcgtggacg
     5521 tcgccaacct ctacgccaac gcgcacaacc tcggcaccga ccccgctgcg ttcctggaca
     5581 ccgtgccgct ggaacgagtc gcttacgtgc acgtcggtgg cggtgtctgg cgcgacggca
     5641 cctaccacga cacgcacgct cacccggtgt tgccggaagt gctggaactg ctctgcgagc
     5701 tgggtgcgcg cacccggccg cgcggggtgc tgctggaacg ggacgaccgg ttccccgatg
     5761 acgacgagct ggccgccgag ctcgccgcga ttcgccgggc ggtggcgcgg tgagccggga
     5821 gcggctggcg gcccagcaag cggaactgct gggcgcgttg ctcggtcagc gccaggaccc
     5881 gccggggttc gaccgcgcgc agttgcgggt tcagcagcag gcgttgctgg acaagcggcg
     5941 gcgagtcgtc gcgcggctgc gtccggactt gccggaactg ctcggcgacc ggttccggtc
     6001 gttgttcgag cgctacgccg cggagcagcc gcgcgcggcc ggttccaaag ctcgcgcgga
     6061 tgccgcggaa ttcgccggtt ggctttcccg gcgcggggag ctgcgccgcc ggaggtggct
     6121 gccgcgccgc tgaccgcggt cttcgtgcgg ccgcggcggc gtgcgcgcgt ggctgcgcgc
     6181 gcacgccgcc gggaagcggt tcacctgcgc cggagcgctc tggcgatcag tcggccgatg
     6241 accaggtaga ccagcgccgc caccccgtag ttgagcacga cgcccagcag cgcgtcgttc
     6301 ggcgtgaaca cgtcgcccaa gcccagcacc agcgtcttgg ccagggtgta gacggcggag
     6361 acgaattggt ttccctggtt cgcgtcgaaa acgacgaaca ggatgtgcag cacgaaagcc
     6421 agcacgatca cgctggtgag gacgtcgacg atgttggcga gcgtattggc gccggagtcg
     6481 ccgcggtcgc cgcgcccgtc cctggagcgg ccttccggtg gccggtcgta atagccgtcc
     6541 ggcggcgcac cgtgatcgta ctggtgcggc gggtaaggat tctggtcgta cccgtaatgc
     6601 ggtccgtgcc cgggcggggg cggatttccg tactgcgggt actgattagg gccgcgctgg
     6661 ggatggttca tgggcggcga ttgtgcggac cgctgacagg cggcaacaac cccgcgccgg
     6721 tcgcgcgcga agtgggcggg acggcgcagt aacgcgcgac gaacgcgaaa atcgttcggc
     6781 acccccgacc gtgccagcgg aaacgattgt tgaaatccgc aacggattag tcgatgaatg
     6841 caacgagtta ttcctgcgtg atggccgcgt ttgcgcatcg ttgcttttcc gtgtggtttt
     6901 cgtcctaacc cggcacgcgc attttggcca tgcttgcgcg ccgcacggca caggacaacg
     6961 gtgcgccgct gggcgcgaat tcgagggcgg aatgacttac acagcgcgac agccgaatga
     7021 cgcaccgccg cggcgaactc gccggaaacg gcgtaccgga cgaaacgtcc tgatcgtcct
     7081 cgtcgtgctg ctgctggccc tggccggctt cggcctgtac gtggacatgt cgttgaagcg
     7141 ggaggacgcg ttgcccgcgg gcggcgaacg gcccgctgcc ggtcccggca cgaactggct
     7201 gctggtgggt tcggacagcc gcgaagggct ggacgccgag cggcaggccg aactgggcac
     7261 cggcgatgcg gccggtaggc gcaccgacac gatgatgctg gtgcacatcc cggaaagcgg
     7321 cggggagccg acgatggtga gcctgccgcg cgactcctac gtgcggatcc cgggcaaggg
     7381 caaggacaag ctcaacgcag ccttcgccta cggcgggccg gagctgctca gccgcaccgt
     7441 cgagggcgcc accggagtgc gcatcgatca ctacgcggag atcggcctcg gcggcttcgc
     7501 cgacatcacc gacgccgtcg gcggcgtcga cctgtgcgtc aaggagcgga tgaccgaccc
     7561 gaaggcgagc ttggacctgc agcccggttg ccagacggtg aacggcccgc aggcgctggg
     7621 ctacgtgcgc agccgcgcgt cctcccgcgg cgacctcgac cgcgtcgagc accagcggga
     7681 attcctcgcc gcgctgaccg agcgggcgtc gagtccgcag gtgctgctca acccgttccg
     7741 gttgttcccg ctgatcaccg acacctccgg ggcgttcctg gtcggtcgcg acgagcacgt
     7801 gtggcatctg ggcgccctgg cgttcgcgat gaacgggatc gcctccggca agggcatcac
     7861 gaccacggtg ccgatgagcg gtttcggcga gagcagcgac ggcgcgtcgg tgatcaagtg
     7921 ggatgcggag aaggccgccc agctgttcgg cgcgctggcg aaggacgagc cggtgcccgc
     7981 gggcgcggcg ggcgccgggt tcggcggctg aacccgcggc cgggcccgcg tcgtcgcgcg
     8041 ggcccggcct cagtcgtcgc gcgcaccggt gtcgagcacc gcgaccgccg cgttcgtgac
     8101 cagctcgcgg tcctgctcgg aaaggcgcgg accgacgtcg gcctccggcg gtcggttcag
     8161 cgccagcgag tcgatgtcgc cggtggagat gatcgcgccg cgcaccgtgc cgatcgcgca
     8221 ccgggccagc agcaggtcgg ccgggtcgtc ggaggctgcc aaggcgttct ccagcgcccg
     8281 gacgtcggcg aacatgtcgt ccttggccag cagcacgctg cgcgccgaag cgtcgtagag
     8341 ggtgccgcgg atgatgtgcc ggtggtcgtc gaagaggtcg acgacccggc gcagcagccg
     8401 ccgcgaggat tcgctgccgt cctcggccgc ccgcacgcac gcgccgagct cgtcgacgac
     8461 cggcgcgacg agctcgcgca gcaggtcctc cttggccggg aagtggtagt acagcgcggc
     8521 tttcgtcatc tccaggcgct cggcgatgtc ccggatcgac gtgcccgcgt agctgcgctc
     8581 ggtgaacagc tcgcgggccg cttgcagcac ccgcgcccgg gtgcgggccg cggagctacc
     8641 ggtcgttcgt gccatttcgg acctcctcga ggggcagagg ctagcggaca gggtcagctt
     8701 gacggccgga gtaatctacc gaccgtccga ttaatacagc cagggagcga tgcatgacca
     8761 atcggtccgg agccggaatc gacatcggaa gcgtcgactt cgagtccttc taccgaggtg
     8821 aaggcgggtt cgccgacatc gagttcgacc gcgcgccgtg ggacatcggc gagccccagc
     8881 cggtgctcgt gcagctggag cgggacggcg gcatcaccgg ccgcgtgctc gacgccggat
     8941 gcgggcccgg cgacaacgcg atcttcctcg ccgagcgcgg ctattccgtg accggtgtgg
     9001 acggatcggc gacggcgctc ggaacggcgc gtgaacgcgc ggccgcccgc ggggtccggg
     9061 ccgacttcca ggtcgccgac gcgaccacca tggaaggggt tccgcaggag ttcgacaccg
     9121 tcctggacag cgccctctac cactgcctgc ccgcggagtc gcgcgccgcg tacggcgcag
     9181 cgctgcaccg ggtcaccaaa cccggcgcca agctgcacgt gctctgcttc gccgacgtcg
     9241 aagacgcacc ggcgcccgcg acggtcagcc aggacgacct gcgcacgaac ctcggcgagc
     9301 actggcggat caacgcgatc gagcaggtcc gctactccgg ggcgttcgac ccggaaaccg
     9361 cggcgcgcca tttcgagcaa ttcatgccct tcgacgccgg tgcggcgatc accgacgaca
     9421 acggccggtt caccttcccg atgtggcact tgcaggccga acgggcctga cttccggcga
     9481 gaagcggcgc accgcgccgg cccggccggg catccgcgat tccgcgggtg cccggccgtt
     9541 tcgcgtgcga ccccgcgcac gaatgggctc agcgggtgaa aaccgcacgg aagcggcttc
     9601 cctgatcgtc gacgtcacca caattgcgcg acatcgactg ccacgggggt ccgattgaac
     9661 aacgggacaa cgccgtcggc cgcggtcacc gcggacgacg cagccgggct ggcgcggcag
     9721 cgggagcgga acacgcagcg gctggccacg gccacgggac atgtccccaa gctttcggac
     9781 gcggtgatcg gtgcctacct gcgcagtccc cagcaggcgc tgccgcccga accgggagtg
     9841 gacgcggccg cggtgctggg ccattcactg gccgcccggt accggcgcgg cctccgcgac
     9901 ctggtgctgc tggtgttcag cctggtcatg ggcgtggcgc tgtactacgc ggatgcgggg
     9961 ctcgtgtggt tctggctcgg tttcatcgcc gtttggctgc tcgtgcggct gctggcgcgg
    10021 gttttccggc gcaggcgcgt gccggagcgg ttacgcgccg gggtggcgca cgtgatcgtc
    10081 gcgttcctgc tgtggtcact gctggcgtcg gagttggaag gcccgctgga cgacgcgttc
    10141 gacatcgacg actcgctgca cgcgctgccc gattcgccgc tgtcgtcgtt gctcgtgatc
    10201 gcttggctgg cgggtttgtt cgccgtgctg gtgagtgatc agctgctcgt ctcgggcggc
    10261 accgactcgc cggtcgcgcg gctgggcaag gccgtgcgaa cggtggggac gcggcgtcac
    10321 ggccgcctgc tgggtgagat gtccgcccgc ccggattccg ggaacgtcct ggtgcaatcc
    10381 gggtggagcg ccttcccggg acatggcgac cggctgaacc agtggtcgat ggtgatcccg
    10441 ctgcacaagg aggggctgcc ggaaggcgaa gccggtggtg ccgacgtgcg gccgctcgac
    10501 ctgtaccgcg cggtcgccgc ggaactgcgc cagatgcact cgtccgacct gctggttccg
    10561 ggttcgcggc tgcgcgggct caccgtcgac ccggtggtcg cggtgcacgc gggaacgatg
    10621 tcggcccacg ccgagagccc gctcggccgg acgctgctgc cggaccgggg cgggcgcccg
    10681 gtgcccgcgg ttgctccgga gctggtcgag cagatcgccg accgcgggcc ggagtgggcg
    10741 cggcacttcc agcgcagctc ggtcgccggg tgggaatcgg acgtcgtggt gtccaccttc
    10801 ttccacgtcg gct
//
