LOCUS       NZ_JADDUE010000001     10390 bp    DNA     linear   CON 14-SEP-2024
DEFINITION  Saccharopolyspora sp. HNM0986
            NODE_1_length_979558_cov_78.761708_cov_78.761708, whole genome
            shotgun sequence.
ACCESSION   NZ_JADDUE010000001
VERSION     NZ_JADDUE010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Saccharopolyspora sp. HNM0986
  ORGANISM  Saccharopolyspora sp. HNM0986
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Saccharopolyspora.
REFERENCE   1  (bases 1 to 979558)
  AUTHORS   Li,F.
  TITLE     Diversity and distribution of actinomycetes associated with coral in
            the coast of Hainan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 979558)
  AUTHORS   Li,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2020) chool of Life and Pharmaceutical Sciences,
            Hainan University, 58 Renmin Avenue, Meilan District, Haikou, Hainan
            570208, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 232.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016526145.1-RS_2024_09_14
            Annotation Date                   :: 09/14/2024 02:15:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,899
            CDSs (total)                      :: 5,838
            Genes (coding)                    :: 5,784
            CDSs (with protein)               :: 5,784
            Genes (RNA)                       :: 61
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (5S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 7 of 54
            Pseudo Genes (incomplete)         :: 49 of 54
            Pseudo Genes (internal stop)      :: 0 of 54
            Pseudo Genes (multiple problems)  :: 2 of 54
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:09:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 534089
            Orig. end                         :: 544479
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADDUE010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5390](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5390
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     tRNA            504..576
                     /anticodon=(pos:534626..534628,aa:Thr,seq:tgt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="INP57_RS02430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="INP57_02430"
                     /product="tRNA-Thr"
     gene            504..576
                     /locus_tag="INP57_RS02430"
                     /old_locus_tag="INP57_02430"
     gene            complement(664..1446)
                     /locus_tag="INP57_RS02435"
                     /old_locus_tag="INP57_02435"
     CDS             complement(664..1446)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037170.5"
                     /locus_tag="INP57_RS02435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_02435"
                     /product="VOC family protein"
                     /protein_id="WP_200068318.1"
                     /transl_table=11
                     /translation="MEITEYRPGTPCWVDLGVPDPSTTSEFYAELFGWTVHDTGDEGGG
                     YRMCLRHGKPVAGIGPHSGGSSGPRSWTTYICVRDADAAAEAVRRAAGQVQLEPMDVLD
                     AGRMAVFTDPSGAAIAAWQPNEHIGSAVLDEPGAPCWHELATRDPESAVEFYSAVFGWE
                     AANEQIGAEDYTAWRLEGRTIAGMIQMTASWPTELPAHWTVYFTVADTDAAIEHLDRLG
                     GTVLAGPTDIEPGRFAVVHDPAGGLFGIIAINPAAAPG"
     misc_feature    1591..1606
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.92"
                     /tool="antismash"
     gene            1691..2431
                     /locus_tag="INP57_RS02440"
                     /old_locus_tag="INP57_02440"
     CDS             1691..2431
                     /EC_number="3.4.21.-"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947391.1"
                     /locus_tag="INP57_RS02440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_02440"
                     /product="trypsin-like serine protease"
                     /protein_id="WP_307849861.1"
                     /transl_table=11
                     /translation="MSLVRVLTGAAGLALAAAAFAVPAQAAAPAAPAPPSDLQPMIIDG
                     EDATNAPWAARLLNNGQEACTATAISPEWVLTAQHCVEGAEDGAISFRVGSLNQHEGEE
                     FKAKSGGVHIHDSVDLALVNVDRPMNVEFAPLGETGDVANGDTVQTYGWGATCTDKPES
                     ECQATNLKVADVKVTDIACTDYRGGTAVCAERGTGIPAGGDSGGPMFSNGKQVGVASTS
                     DRATSTAYNNITEYRDWIKEVAGV"
     misc_feature    complement(2759..2770)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            2809..3408
                     /gene="ectA"
                     /locus_tag="INP57_RS02445"
                     /old_locus_tag="INP57_02445"
     CDS             2809..3408
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682485.1"
                     /locus_tag="INP57_RS02445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_02445"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_200068320.1"
                     /transl_table=11
                     /translation="MTGDKPSHNGYGPNGGRNGGSDSPDAEIGIPVVADGPALYRITSD
                     SGVLDVNSSYAYLMWCRDFAGTSVVAREDGEAVGFITGYLRPDAPDVLMVWQVAVDASQ
                     RGRGLAGKLLTHLVDRLVPRGVRYLETTITADNTASIKLFSALARDRGTELARSELFTA
                     DLFPDAHEDEDLYRIGPFPAPGVVPADSAAAPRPAD"
     gene            3649..4896
                     /gene="ectB"
                     /locus_tag="INP57_RS02450"
                     /old_locus_tag="INP57_02450"
     CDS             3649..4896
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (25-353). E-value: 1.1e-70.
                     Score: 230.3. Matches aSDomain:
                     nrpspksdomains_INP57_RS02450_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947388.1"
                     /locus_tag="INP57_RS02450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_02450"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_200068321.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.4e-74, bitscore:
                     246.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDIFASLESEVRSYSRNWPTTFDVARGSWLYGEDGTPYLDFFAG
                     AGALNYGHNNPALKRKMLEYLERDGVTHGLDQATVAKREFLETLDEKLLKPRGLDYKVQ
                     FPGPTGTNSVESALKLARKITGRESIISFTNAFHGMTLGSLSVTGNSMKRGSAGIPLVH
                     ATPMPYDNYFEGQVPDFLYFERLLEDSGSGLNEPAAVIVETLQGEGGINNSRPEWLRGL
                     RELCDKHGMLLIIDDVQMGCGRTGPFFSFEEAGIVPDIVCLSKSIGGYGMPLALTLIKS
                     DLDVWEPGEHNGTFRGNNPAFVTGSEALRLYWSDDALQRGTIAKGERIGQVIGEIADKY
                     EGAQAKGRGLARGLGFDDPETAGKISKAAFDRKMILETSGPQSEVIKIMPPLTVSDDEL
                     EQGLQIIVDSVQAVLA"
     aSDomain        3724..4707
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_INP57_RS02450_Aminotran_3.1"
                     /evalue="1.10E-70"
                     /label="INP57_RS02450_Aminotran_3.1"
                     /locus_tag="INP57_RS02450"
                     /protein_end="353"
                     /protein_start="25"
                     /score="230.3"
                     /tool="antismash"
                     /translation="ARGSWLYGEDGTPYLDFFAGAGALNYGHNNPALKRKMLEYLERDG
                     VTHGLDQATVAKREFLETLDEKLLKPRGLDYKVQFPGPTGTNSVESALKLARKITGRES
                     IISFTNAFHGMTLGSLSVTGNSMKRGSAGIPLVHATPMPYDNYFEGQVPDFLYFERLLE
                     DSGSGLNEPAAVIVETLQGEGGINNSRPEWLRGLRELCDKHGMLLIIDDVQMGCGRTGP
                     FFSFEEAGIVPDIVCLSKSIGGYGMPLALTLIKSDLDVWEPGEHNGTFRGNNPAFVTGS
                     EALRLYWSDDALQRGTIAKGERIGQVIGEIADKYEGAQAKGRGLARG"
     gene            5001..5390
                     /locus_tag="INP57_RS02455"
                     /old_locus_tag="INP57_02455"
     CDS             5001..5390
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441528.1"
                     /locus_tag="INP57_RS02455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_02455"
                     /product="ectoine synthase"
                     /protein_id="WP_200068322.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.2e-56, bitscore:
                     184.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFVRSLEDVEDTDDDIKTENWRSKRIVLAKQKVGFSVHETTLYAG
                     TVNDFWYANHIEAVFVYEGEGEVEDKATGEVHQLKPGTVYMLNDHDKHQVRPKTDMRTV
                     CVFNPPVTGREVHDENGVYPLVVED"
     gene            5694..6596
                     /gene="thpD"
                     /locus_tag="INP57_RS02460"
                     /old_locus_tag="INP57_02460"
     CDS             5694..6596
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="INP57_RS02460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_02460"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_200068323.1"
                     /sec_met_domain="PhyH (E-value: 1e-32, bitscore: 109.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVADVRTQDRYPTRRGAESALLERTDPVVWPGVDSGPAGTGELA
                     EHQDKGFHVLPGLLSPAEVQEHWQELERLVRDPDVRADERTVVEKESNEVRSLFEVHRT
                     SEMVAELIRQPRILDRARQILGSDVYVHQSRINYMPGFRGNGFYWHSDFETWHAEDGMP
                     APRAVSLSIALTDNFPFNGGLMVMPGAHRTYVSCAGKTPDDNYKSSLQEQKVGVPAEDD
                     IRRLGYEHGIEQFTGAAGAALWFDSNTMHGSGNNITPYPRSNIFVVFNSVQNTLVDPFA
                     AKQRRPEFVAATDFTPLGR"
     gene            complement(6643..7680)
                     /locus_tag="INP57_RS02465"
                     /old_locus_tag="INP57_02465"
     CDS             complement(6643..7680)
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873074.1"
                     /locus_tag="INP57_RS02465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="INP57_02465"
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="WP_200068324.1"
                     /transl_table=11
                     /translation="MPTPSAVVDRAVGCLLGVALGDSLGVPVEFQQLDGIRAEFGEQGV
                     AEPPMPALLGDATQMTLFTGEGYLSAWTRGNNGGQWNPVESAAAAYRRWLSTQQEDKPP
                     TGATGLLAEGELYANRSPGLTSLRALGEPQLGSPDRPRNSSKGCGGLDRTAPLGFAPSS
                     ALAYGLGCRFAALTHGGAGGWASAGALSLVVHLIAVQDRRLTEAVDQASGRVLRDDPET
                     AGLLAEASTLARLGGSVGHVERLGQGWIGPEALAIGVYCALALPKAEQFTAALRLAANH
                     SGHSDTTAAITGAILGARHGTAALPRNWLSRLELADVVERVGHDVGASCSGEKFNERRY
                     LGLDT"
     misc_feature    complement(8956..8970)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     misc_feature    9343..9358
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.79"
                     /tool="antismash"
     misc_feature    10241..10255
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 tagcaggaaa ccgtgatcga cgtgtagatc acgtcgacct cgcagtgcgg cagaccgtag
       61 gcgctgacca cgccgatgat cgtggccgtc acgtcggagg cgcccgcacc gctggccatc
      121 tggacctcgc cgatgcgcag tcctaggtcc atgaccaagt ggacggtcga ttcgtcgggc
      181 agtgccggcc cgtaaacggc gttgcgggtc ccgaccgtct cggccggttc gcgccgccgc
      241 agcacaccac gggcgcgctg cgtgattccc accgctgaaa ccaccttcct gctcaccgct
      301 cccggcggct cgccttgatc gtcacctcgc ccatcggcgc cgttgccgtt gctgttgcac
      361 ggacctcggg tgtcctcggt cacgagcctg acacttcgcc tcgcgcgccc gcgtgcgagg
      421 gtcctgcgcg accgcctgct cgcggcgggc gtgcggtgat tacgatgtgt ccgcgtggtc
      481 gccggcatcg ccggttgctg tgcgccgctg tagctcagta ggcagagcgc ccgccttgta
      541 agcggatggt caggggttcg aatcccctca gcggctcccg gttcgagcag cctaagcgcg
      601 tcctgccctt cggcgacagg acccgatcga ggcattgatc gcccccggcc gcgagccgcg
      661 gcgtcaaccc ggcgcggccg cggggttgat cgcgatgatg ccgaacagcc cgcccgccgg
      721 gtcgtgcacc acggcgaacc ggcccggctc gatgtcggtg gggcccgcga gcacggttcc
      781 gccgagccgg tcgaggtgct cgatcgcggc gtcggtgtcg gcgacggtga agtagaccgt
      841 ccagtgcgcg ggcagctcgg tgggccagct cgcggtcatc tggatcatgc ccgcgatcgt
      901 gcgcccctcc agccgccagg cggtgtagtc ctccgcgccg atctgctcgt tggccgcctc
      961 ccagccgaac acggcggagt agaactccac cgcggattcc gggtcgcggg tggcgagttc
     1021 gtgccagcag ggggcgccgg gttcgtccag cacggccgag ccgatgtgct cgttgggctg
     1081 ccacgccgcg atggccgctc ccgaggggtc ggtgaacacc gccatgcggc cagcgtccag
     1141 cacgtccatc ggctccagct gcacctgccc cgccgccctg cgcaccgctt ccgctgcggc
     1201 gtcggcgtcg cggacgcaga tgtaggtggt ccacgaccgc gggccgctgc tgccgccgga
     1261 gtgcgggccg atgcccgcga cgggtttgcc gtggcgcagg cacatccggt agccgccgcc
     1321 ttcgtcgccg gtgtcgtgca cggtccagcc gaacagctcg gcgtagaact cggatgtcgt
     1381 gctggggtcg ggcacgccga ggtccaccca gcacggggtg cccgggcggt attcggtgat
     1441 ctccatgggt gtccccgctg ctcatcgagg tgcggccgcc gaggtgcggg agacgtccga
     1501 ccggcgccat agtgggcacc cgattgttgg ttgagtccgt cgatcggcgc atctcgatca
     1561 gaactcagct cgcagtcgcg gtggcgaata gtgaccaatt ggttgaccca ttacccgttc
     1621 gggtgagggt atggagcgtg ttcgaagccg ctccgtacag cgttgccgct gctacccaga
     1681 ggagatcagc gtgtctctgg tccgagttct gaccggcgct gcgggcttgg ccctggccgc
     1741 cgccgcgttc gccgtcccag cccaagccgc ggctcccgcg gcgcccgctc cgccgtccga
     1801 cctgcagccg atgatcatcg acggggagga cgccacgaac gctccctggg cggcccgcct
     1861 gctcaacaac ggccaggagg cgtgcaccgc cacggccatc tcgccggaat gggtgctgac
     1921 cgcccagcac tgcgtggaag gcgccgagga cggagccatc agcttccgcg tcggcagcct
     1981 caaccagcac gagggcgagg agttcaaggc caagtccggc ggcgtgcaca tccacgacag
     2041 cgtggacctc gcgctggtca acgtggaccg cccgatgaac gtcgagttcg cgccgctcgg
     2101 cgaaaccggc gacgtcgcca acggcgacac cgtgcagacc tacggctggg gcgcgacctg
     2161 caccgacaag cccgaatccg agtgccaggc gaccaacctg aaggtcgccg acgtcaaggt
     2221 caccgacatc gcctgcacgg actaccgcgg cggcaccgcg gtctgcgccg agcgcggcac
     2281 cggcatcccg gcgggcggcg actccggcgg gccgatgttc tccaacggca agcaggtcgg
     2341 ggtggcctcg accagtgacc gggccacctc cacggcctac aacaacatca ccgaataccg
     2401 ggactggatc aaggaggtcg ccggcgtctg atcgacgccg gatgccgtgc gtccggggcc
     2461 gccgcactcg cggccccgga cgcacgtgtt cggtcgcggc cgcctgccgt gcgcggccgc
     2521 tcgcgcggcg ccgctgaaga tcgcaattcc gcgcgttatc cgaaatatcg gcggatgcaa
     2581 tccgcgggct gcgggcgaac agcttgtgaa gcgggcgttt tcgctggtca cgcccgcact
     2641 ggggaatttc gatcactagt tccgacacat cgttaacact gcggagatgc gcagcgcaac
     2701 gatcatgtcg tgctcatcac gtgtagaatc gggtatcgcc tggtagtgcc gccaggaaac
     2761 gttcatgtac ctgacggctt aggcgatgtt ctgttaagtt ggcaaagcat gaccggcgac
     2821 aaacccagcc ataacggcta cggaccgaat ggcggacgga atggcggaag cgactctccc
     2881 gatgcggaga tcggcattcc ggtcgtcgcg gacggacccg cactctaccg gatcacgagc
     2941 gattcgggag tgctcgatgt gaactcgtcc tacgcctacc tgatgtggtg ccgcgacttc
     3001 gcgggcacca gcgtggtggc gcgggaggac ggcgaagcgg tcggcttcat caccggatac
     3061 ctgcggccgg acgcgccgga cgtgctgatg gtctggcagg tcgcggtcga cgcatcccag
     3121 cgcgggcgcg gcctcgcggg caaactgctc acgcacctcg tcgaccgcct ggtgccgcgg
     3181 ggggtgcgct atctggaaac gacgatcacc gcggacaaca ccgcttcgat caagttgttc
     3241 tcggcgctgg cgcgggaccg cggcaccgaa ctcgcccgca gcgagctgtt caccgcggac
     3301 cttttccccg atgcgcacga ggacgaggac ctttaccgaa tcgggccgtt cccggctccg
     3361 ggcgtcgtgc ccgcggactc ggcggcggcg cccaggccgg ccgattagcc gaggcgtacc
     3421 gatcagcaca ggcagaccga tcagcccggc ccggcccccg tggtccagcg ggccgatcgg
     3481 cacacgcggt gagcgcgggc cggggcgatc gcgaagcagc ccggcggccg tgccggatcg
     3541 cacaaccgca ggttcccggc gcaccaacgg cggtctcccc gccgggagac gagaagacct
     3601 actgcacagc cggtggccgc caacggtcgc cgcgccgaac ggaggatcgt gaacgacatc
     3661 ttcgcaagcc tcgaatccga ggtgcgcagc tacagcagga actggccgac cacgttcgac
     3721 gtggcccgcg gcagctggct ctacggcgag gacggcaccc cctacctgga cttcttcgcc
     3781 ggtgcggggg cgctcaacta cgggcacaac aaccccgcgc tgaaacgcaa gatgctggag
     3841 tacctggagc gcgacggggt cacgcacggc ctggaccagg ccaccgtggc caagcgggag
     3901 ttcctggaga cgctcgacga gaagctgctc aagccgcgcg ggctggacta caaggtccag
     3961 ttcccggggc cgacgggcac gaactcggtg gagtccgcgc tcaagctggc ccgcaagatc
     4021 accggccgcg agtcgatcat cagcttcacc aacgcgttcc acggcatgac gctgggttcg
     4081 ttgtcggtga ccggcaactc gatgaagcgc ggcagcgcgg gcatcccgct ggtgcacgcc
     4141 acgccgatgc cctacgacaa ctacttcgag ggccaggtcc cggacttcct gtacttcgag
     4201 cggctgctgg aggacagcgg atccggcctg aacgagcccg ccgcggtcat cgtcgagacg
     4261 ctgcagggcg agggcggcat caacaactcc cgcccggagt ggctgcgcgg cctgcgcgaa
     4321 ctgtgcgaca agcacgggat gctgctgatc atcgacgacg tgcagatggg ctgcgggcgc
     4381 accggcccgt tcttcagctt cgaggaagcg ggcatcgtgc cggacatcgt gtgcctgtcg
     4441 aagtccatcg gcggctacgg gatgccgctg gcgctgaccc tgatcaagtc cgacctggac
     4501 gtgtgggagc ccggtgagca caacggcacc ttccgcggca acaaccccgc gttcgtgacg
     4561 gggtccgagg cgctgcggct gtactggagc gacgacgcgc tgcagcgcgg cacgatcgcc
     4621 aagggcgagc ggatcggcca ggttatcggc gagatcgcgg acaagtacga gggcgcgcag
     4681 gccaagggcc gcggcctggc ccgcgggctc ggcttcgacg acccggagac cgcgggcaag
     4741 atctcgaagg ccgcgttcga ccggaagatg atcctggaga cctccgggcc gcagagcgag
     4801 gtcatcaaga tcatgccgcc gctgaccgtc tccgacgatg aactcgaaca ggggcttcaa
     4861 atcatcgtgg attcggtgca ggctgtgctt gcctgaatcc agcgcacacc ggccggcccg
     4921 gctgtctccg ggccggccgc tcgcgtccgc actaccgaag ctggcaaacc cgaagccggc
     4981 ccaccgaagg agggaaagca gtgttcgtcc gcagtctcga agacgtcgag gacaccgacg
     5041 acgacatcaa gaccgagaac tggcgcagca agcgcatcgt gctggccaag cagaaagtcg
     5101 gcttctccgt gcacgagacc acgctgtacg ccggcacggt gaacgacttc tggtacgcca
     5161 accacatcga ggcggtcttc gtctacgagg gcgaaggcga ggtcgaggac aaggccaccg
     5221 gtgaggtgca ccagctcaag ccgggcaccg tctacatgct gaacgaccac gacaagcacc
     5281 aggtgcggcc gaagacggac atgcgcacgg tgtgcgtgtt caacccgccg gtgaccgggc
     5341 gcgaagtgca cgacgagaac ggcgtttacc cgctggtcgt cgaagactga ccgacccccc
     5401 cgggcgaatc ttgcagccca cgggccgctg cggcggctcg tgggctgttt cgcgttcggg
     5461 cgctgatcgc atttcgccct tcgtgatcga tcccaccggc cgccgggctc gaagactccg
     5521 ctgcgcggat gcgtaatgcc gagtgccgag gggtatccgg cgagtgtcag gttgcctgcc
     5581 gcggtgcgcg gccccgcatt cggggaaacg ccaccgaacg aggaaccgag aaatggcggc
     5641 ggtgcgtgcg gcaccggccg ctccggcggg aattcgcgaa ggaggcgcaa ctgatgaccg
     5701 tcgcagacgt gcggacgcag gaccgctatc cgacccggcg cggagccgag tccgcgctgc
     5761 tggaacgcac cgatccggtg gtctggcccg gcgtggattc cgggcctgcg ggcaccggcg
     5821 aactggccga gcaccaggac aagggcttcc acgtgctgcc gggcctgctc tcgccggcgg
     5881 aggtgcagga gcactggcag gagctggagc ggctcgtccg cgacccggac gtccgcgccg
     5941 acgagcgcac cgtggtggag aaggaatcca acgaggtccg ctcgctgttc gaagtgcacc
     6001 gcaccagcga aatggtggcg gaactgatcc gccagccgcg cattctcgat cgcgcgcggc
     6061 agatcctcgg gtccgatgtc tacgtgcacc agagccggat caactacatg ccgggattcc
     6121 gcggaaacgg tttctactgg cattccgatt tcgagacctg gcacgccgag gacggcatgc
     6181 ccgcgccgcg cgcggtgagc ctgtcgatcg cgctgaccga caatttcccg ttcaacggcg
     6241 ggttgatggt gatgccgggc gcgcaccgca cctatgtttc ctgcgcggga aagacgccgg
     6301 acgacaacta caagtcgtcg ctgcaggagc agaaggtcgg cgtccccgcc gaggacgaca
     6361 tccggcggct cgggtacgag cacgggatcg agcagttcac cggggccgcg ggcgcggcgt
     6421 tgtggttcga ctcgaacacg atgcacgggt cgggcaacaa catcaccccg tacccgcgct
     6481 cgaacatctt cgtcgtgttc aacagcgtgc agaacacgct ggtcgatccg ttcgcggcga
     6541 agcagcgcag gccggagttc gtcgccgcca cggacttcac cccgctcggc aggtgagcgc
     6601 ctccgcggtc cggcccccgc gagggggccg gacctgcgtt gctcaggtgt ccaggcccag
     6661 gtagcggcgt tcgttgaact tctccccgga gcacgacgcg ccgacgtcgt gccccacccg
     6721 ctcgaccacg tcggccagtt ccaaccggga cagccagttc cgcggcaacg cggcggtgcc
     6781 gtgccgggcg ccgaggatcg cgccggtgat cgcggcggtg gtgtcgctgt gcccggagtg
     6841 gttcgccgcc agtcgcagcg ccgcggtgaa ctgctccgcc ttgggcagcg ccagcgcgca
     6901 gtacaccccg atggccagcg cctccgggcc gatccagccc tgaccgagcc gctccacgtg
     6961 gccgaccgag ccgccgagcc gggccagcgt ggaagcctcc gcgagcaggc ccgccgtctc
     7021 cgggtcgtcg cgcagcacgc gcccggaggc ctggtcgacg gcctcggtca gccgccggtc
     7081 ctggaccgcg atcaagtgca cgaccagcga caacgcacct gcggacgccc agccgcccgc
     7141 gccgccgtgg gtcagcgccg cgaaccggca gcccagtccg taggccagcg cggaactggg
     7201 cgcgaaaccg agcggggcgg tgcggtccag cccgccgcat cccttcgagc tgttccgcgg
     7261 ccggtccggg ctgcccagct gcggttcgcc cagcgcgcgc aggctggtca ggcccgggga
     7321 gcggttggcg tagagctcgc cttcggcgag cagtccggtc gcgcccgtcg gcggtttgtc
     7381 ctcctgctgc gtggacaacc agcgccggta ggccgccgcg gccgattcga ccgggttcca
     7441 ctggccgccg ttgttgccgc gggtccacgc gctcaggtag ccctcgccgg tgaacagcgt
     7501 catctgggtc gcgtcgccga gcagcgcggg catgggtggc tcggcgacgc cctgttcgcc
     7561 gaactccgcg cggatgccgt cgagctgctg gaactcgacc ggcacgccga gcgagtcgcc
     7621 gagcgcgacg ccgagcaggc agcccacggc gcgatcgacc actgcggacg gggtgggcac
     7681 gcggcgggct ccttcgggtg gtgctcgacg atgatcgatc tggtgcgagg gtactgggca
     7741 cctccgcgaa ccgggagccg atcggagcag cgccggcgcc gtccgggggt gagtccgggc
     7801 acggtgcgcc actgctgcga cccttcggcc gcgtgctgag ctgcaccgga accggagatc
     7861 caccgcgggc ggccgcgccg ctcgctgggc cgatcgggtg agctagtcgg cggtgccgca
     7921 ggtgctcgcc gcgccgctcg ctagcgtgtg tcggtttcgc cgctagtgga ctcgccgccg
     7981 cgcggtgccc gaaactgacc aggaccggat caagcgcagg aacggcaggt gtgcacggga
     8041 tgccagggca ggacgcctcg ctcggcggag ggggcagcgg attcaccttc cgcgtgctcg
     8101 gcccgctgga ggtgcgcctg gacggcgtcc cgctgcgggt ggggtcggcc gcggcgcgcg
     8161 gcgcgctcgc ctgcctgctg ctggagccga accacgtcgt gccgatggac cggttgatcg
     8221 acatcctctg gggggaagca ccgcccgcga ccgcgcggac catcgtgcac ggctacatcg
     8281 cgcgcatccg ccggatgctg gaacagcagc acgaggacga gcccggcagg cccgcggtgc
     8341 tgacccggtc gcccggctac ctgctgcggg tggacaacga ccggatcgac gcgcaccggg
     8401 cgcgcacgct ggtcaaccag gcgcacgggc tggaaccggc gaaccgcgcc aagctgctca
     8461 acgaggcgct cgacctgtgg cgcggaccgg tgctgtccga tgtgggcgcc gaacggctgc
     8521 accagatggt cgggccgaac ctcgacgagc tgcggctgct ggcgctggaa gaacgcatct
     8581 cggcggacct ggagctgggc aagcaccacc ggctcgtggt cgaactggcg aacctggtcg
     8641 acgagcaccc gctgcgggaa cggctgacct acctgctgat gctcgccgcg taccgcgccg
     8701 ggaaccgcgc cgaggcgcaa gcctcctacc acgcgctgcg gcaacggctc tccggcgaac
     8761 tcggcatcga ccccggcccg gaggtgcgca ccctctacga acggctgctg cgcgatgacg
     8821 agacgctgct gggcggtgtg cggccgagca ccggccggga ctcggctccg atccggccgg
     8881 tgcccgccga gctgccgccc gcggtcgccg gcttcgtcgg ccgggagagc gaactcggcg
     8941 tgctggaccg gatgctggcc gaacgggacc ggggcggcgc caccatgctc ggcgtgctca
     9001 ccggcaccgc gggggtgggc aagagcgccg tggcgatcac ctgggcgcac cgggtggccg
     9061 gttcgttccc ggagggacag ctctacgcct cgttgcgcgg tttcgactcc gagcgggcgc
     9121 cgctgtcccc cggcgaagcg ctgaccagca tgttgaaggc gctcggcgtg gccgccgacg
     9181 cgatcccgct ggacgtcgac gaccgggccg cgctgtaccg ctcgctgctg gccgaccggc
     9241 gggtgctggt gctgctggac aacgcgcggg actccgacca ggtccggccg ctgctgcccg
     9301 gcagctcgac cgcgctggtg ctggtcacca gcaggcgcag gctggacggg ctggtcgtgc
     9361 gcagcggggc gcgagtgctg ccgctggaga cgctgccgac cggatccgcc gtcgcgctgc
     9421 tggaccgggc cggcggtacc gagggttccg cggccgagcc ggacgctgcc cgcgaactcg
     9481 ccgaactctg cggcgggctg ccgctggcgc tgcgcatcgc ggccgcccgg ctggccgcga
     9541 acccggcgcg cggcgtggcc gacctggtgc acgagctcac cgacgagagc aaccggttgc
     9601 acgggctgga catcgacgac gccgacacca gcgtgcggcg ggcgttcgac atctcctacc
     9661 ggaacctgca tccggtgcac gccagcacgt tccggctgct cgggctggtg cccgggcaaa
     9721 cgttcaccgc gcaagccgtg gcagcgctgt gcgagaccga ctcggcgggc gcctaccgca
     9781 ggttgcgcgc gctggcgctg gcgcacctgg tcaccgagac cgagccggac cggttcggga
     9841 tgcacgacct gctgcggctg tacgcgcggg aactgctcaa cactccggag gagcggacgg
     9901 gcgagcggga cggcggcgcg ttgcagcggt tgctgcacta ctacctggtg gtctccgacc
     9961 acgcccgcag gttcctgcgg cccgcgcggg acgacgtcga cttctccggc gactccacct
    10021 tcgagcagcc cgccatccgt aaccgcaccg aagcgctggc ctggttcgac gcggaatggc
    10081 cgaacctggt cgccgcggtg cgcaccgcga aggcccaggg cctgcaccag cacgcctggc
    10141 agctggtgcg ctgccagttc aactacctgg tggtgcgctg cccctgggag gactggatcc
    10201 aggtctacgg ggacgggctg gaatccgccc ggctcgccgc cgacccgatc ggccaggtgc
    10261 tgatgcccgc cgggctcggc gtcgcctacg cccgcagcgg gcggcacgag gaggcgctgg
    10321 tgcactacgc ggagtcctac cgctgcgcgg tcgccaccgg cagccccagc tggctggcga
    10381 tggcgcaggt
//
