LOCUS       NZ_CP066763            10387 bp    DNA     circular CON 30-NOV-2024
DEFINITION  Burkholderia ambifaria strain BJQ0010 chromosome 2, complete
            sequence.
ACCESSION   NZ_CP066763
VERSION     NZ_CP066763.1
KEYWORDS    RefSeq.
SOURCE      Burkholderia ambifaria
  ORGANISM  Burkholderia ambifaria
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae; Burkholderia; Burkholderia
            cepacia complex.
REFERENCE   1  (bases 1 to 2704441)
  AUTHORS   Xu,Y.
  TITLE     Complete genome sequence of Burkholderia ambifaria BJQ0010
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2704441)
  AUTHORS   Xu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) School of Food and Chemical Engineering,
            Beijing Technology and Business University, No. 11, Fucheng Road,
            Haidian District, Beijing 100048, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016458785.1-RS_2024_11_30
            Annotation Date                   :: 11/30/2024 03:04:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,675
            Genes (coding)                    :: 5,537
            CDSs (with protein)               :: 5,537
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 88 of 138
            Pseudo Genes (incomplete)         :: 78 of 138
            Pseudo Genes (internal stop)      :: 12 of 138
            Pseudo Genes (multiple problems)  :: 34 of 138
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:03:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2011688
            Orig. end                         :: 2022075
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066763.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10387
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="8"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10387
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10387
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5387](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5387
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(353..643)
                     /locus_tag="JG536_RS24985"
                     /old_locus_tag="JG536_24985"
     CDS             complement(353..643)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659968.1"
                     /locus_tag="JG536_RS24985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_24985"
                     /product="putative quinol monooxygenase"
                     /protein_id="WP_175668318.1"
                     /transl_table=11
                     /translation="MSAHVIVALVEAKQGYEAVLVQAQRELVEMARHQPGCLSYELHES
                     LEQPGTVVFFEQWLDRASWEHHMRSPLMDAFRGSAGRWIENFQLLHLRQVA"
     gene            complement(658..1452)
                     /locus_tag="JG536_RS24990"
                     /old_locus_tag="JG536_24990"
     CDS             complement(658..1452)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482610.1"
                     /locus_tag="JG536_RS24990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_24990"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_175668319.1"
                     /transl_table=11
                     /translation="MDLQLQGKTAIVTGATAGIGMAIARTLVGEGVSVTITGRHRDKLD
                     EAVAQITAAVPEGSVRGIVADVSGPRGVAELIDAQPDTDILVNNLGYYEAKPFAEITDD
                     DWLRMIDVNVMSGVRLSRHYFPRMLAKNWGRVIFMSSEVGAFTPPDMVHYGVSKSAQLA
                     VSRGMAELTKGTGVTVNSVLPSATRSEGIVAYLRQTAPRPGMSDAEIESHFFETYRPSS
                     LIARMIEADEIAAMVALLASPLGAASNGAAVRVEGGTFRSIL"
     gene            complement(1510..2343)
                     /locus_tag="JG536_RS24995"
                     /old_locus_tag="JG536_24995"
     CDS             complement(1510..2343)
                     /GO_function="GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494212.1"
                     /locus_tag="JG536_RS24995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_24995"
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="WP_233410200.1"
                     /transl_table=11
                     /translation="MRKLAVAAMLGIAAYSAHAANVLVVLSDSDHLDLKDGKVFATGFY
                     LNELMQPVKALLDAGHQVTFATPEGKAPTMDATSADKMYFNGDEKAMRDYRALLDKLQI
                     TSRAHSPVISLARVEQIGYGHFDAVYVPGGHAPMQDLLTSPAVGRLLADFHARGKTTAL
                     VCHGPIALLSTLPDAQGFTRQLAAGKAGDGAPKWIYAGYRMTVISNAEEEQAKALLGGG
                     AMKFYPQTALQRAGGIYSSNEVPFTAHMVTDRELITGQNPASALDVAGEMLKRLK"
     gene            complement(2374..3087)
                     /locus_tag="JG536_RS25000"
                     /old_locus_tag="JG536_25000"
     CDS             complement(2374..3087)
                     /GO_function="GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006494213.1"
                     /locus_tag="JG536_RS25000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_25000"
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="WP_175668321.1"
                     /transl_table=11
                     /translation="MNRDPRDRQGLTVLFVLTSHATKGATGEPTGFYLGEVTHPLAELD
                     AAGIPVEFASIQGGEPPVDGLDLTDEVNARYWNDSAFRDALRHTLRLGDVDASKYAAVF
                     FAGGHGAMWDFPGNADVQKVTRAVYEAGGVVGAVCHGPAALVDVTLGDGTYLVAGKNLS
                     AFTDEEERAVQLDHVVPFLLASTLTQRGAHHHPAPSWTAKVVVDGRLVTGQNPQSAAGV
                     GAAIRYLLLNRSEQA"
     gene            3209..4117
                     /locus_tag="JG536_RS25005"
                     /old_locus_tag="JG536_25005"
     CDS             3209..4117
                     /GO_function="GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659964.1"
                     /locus_tag="JG536_RS25005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_25005"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_175668322.1"
                     /transl_table=11
                     /translation="MARRFDHLGDVEAFVTVIEKGSMTAGAVALGSTPSVLSRAITRLE
                     SRLGVQLLRRSTRRLSLTEQGRLYLEQSRAAFLLIDDAERAIQGHGGTLTGHVRLSVPT
                     TYGHYRLPPLLRQFTQQHPEVRVELNIANRNVDLVAEGFDLAIRLGELPDSGLVARKLE
                     DAALCLVASPEYVARAGMPENVADLAHHLCLPFMLPSSGRIAPWVFRDGGQDIDWLPPS
                     NVAVSDDVLGVVSMAQSGAGICQTYDFIVQDRVRAGSLVEVLPHLRGRTRAFSVIYAPH
                     RRLSAASRALIDLIVASTGDQ"
     misc_feature    complement(4760..4776)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 22.54"
                     /tool="antismash"
     misc_feature    complement(4879..4895)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 22.04"
                     /tool="antismash"
     misc_feature    4971..4984
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.97"
                     /tool="antismash"
     misc_feature    4972..4985
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.56"
                     /tool="antismash"
     gene            5001..5387
                     /locus_tag="JG536_RS25010"
                     /old_locus_tag="JG536_25010"
     CDS             5001..5387
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659963.1"
                     /locus_tag="JG536_RS25010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_25010"
                     /product="ectoine synthase"
                     /protein_id="WP_165752306.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.2e-31, bitscore:
                     105.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFVRNLRDVEKDGLLVEWGSGTSHRLLTEKDGMGYGVCHTVVRAG
                     TESLLQYRNHLEACYCIAGEGEVEDMNGNVFPIRPGTIYVLDRHDQHYLRGGKNGDLVL
                     VSVFNPPLKGHERHNLGSAEASAY"
     gene            5495..6715
                     /locus_tag="JG536_RS25015"
                     /old_locus_tag="JG536_25015"
     CDS             5495..6715
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659962.1"
                     /locus_tag="JG536_RS25015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_25015"
                     /product="MFS transporter"
                     /protein_id="WP_175668323.1"
                     /transl_table=11
                     /translation="MRAQHQAFFHSLFLARLADQILLFLVPLVIFQMTGRVSWSGIAFS
                     LETLPRYLCFPFLGALCDRYSPTKLMRVSQVLRAAVCVAGVIAYDLVGGIGWLIALSAL
                     CGMLTSQGIVAREVMLPQIFSSHRFEKVLSYSQLADQLGLVLGPMLAAILLSWWRWEFV
                     VVAAAVLFVTADVALAYWQKASAFEFVMPEGTHGTWFTPIKTALSHVIRLPGLKSVIML
                     AAAENLVIGVTLATSAAMVTGFHHESGRYYAGLQTAGACVTIAILLMTARLHVPRRVLG
                     LVGFGSICIGGVIAAASPGIAGYVFGFLLIVGFDKMFNVYIRSTRQKIIPARDYGKTTG
                     VVILLNNMTQPLAGVAVGAFSTRFGPDTVIMVLSVCMGCLGLLVSLAPRIRKGHRPSSA
                     SVSSHEG"
     gene            6705..8228
                     /locus_tag="JG536_RS25020"
                     /old_locus_tag="JG536_25020"
     CDS             6705..8228
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (13-414). E-value: 5.5e-79.
                     Score: 257.6. Matches aSDomain:
                     nrpspksdomains_JG536_RS25020_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.5"
                     /locus_tag="JG536_RS25020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_25020"
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="WP_199569575.1"
                     /sec_met_domain="AMP-binding (E-value: 5.3e-80, bitscore:
                     266.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKADAMIERFPQYFERSASSRRDKVALVVDGRSATYGELLDVMLT
                     IADTLHRLGIPAGARVAIYSEMSVHAVAAAFGILKAGCVLACIRHTIDTAELEQQIADC
                     GASALVASRATSPRIASRYRAVTIDATRGDLSEYVVCFAADGASEAGNSSMDDAATIFY
                     TSGSTGDSKGVLVTHRIMIAAFRSVSGYLANSERDIVLSFSPGLSSDYGFYNTVMPLLF
                     GGRAVVETALPASAAAIVDIIERHGVTGLHVFPPALFRLCESGDLPASRLQSLRYVSTS
                     GQAFPGKYIRLLRRALPAVLIYCNYGSTECKRIAYLPPAELDRRIGSVGKAIPGIRTYL
                     VGNDNALVVRPWEVGELAVAGDLLMEKYWGRESDTARVIREGCFGESRVFMTGDLFAMD
                     EDGFLFFQCRKGDLFDRDGVPVNPRAVERVLLDHETVAEALVVPVSRSDGARVPKAYVV
                     PAATSAANAIELLDHCRRHLDSAAVPASIDFLRALPRTFGGKVSAQGLN"
     aSDomain        6744..7946
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_JG536_RS25020_AMP-binding.1"
                     /evalue="5.50E-79"
                     /label="JG536_RS25020_AMP-binding.1"
                     /locus_tag="JG536_RS25020"
                     /protein_end="414"
                     /protein_start="13"
                     /score="257.6"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FERSASSRRDKVALVVDGRSATYGELLDVMLTIADTLHRLGIPAG
                     ARVAIYSEMSVHAVAAAFGILKAGCVLACIRHTIDTAELEQQIADCGASALVASRATSP
                     RIASRYRAVTIDATRGDLSEYVVCFAADGASEAGNSSMDDAATIFYTSGSTGDSKGVLV
                     THRIMIAAFRSVSGYLANSERDIVLSFSPGLSSDYGFYNTVMPLLFGGRAVVETALPAS
                     AAAIVDIIERHGVTGLHVFPPALFRLCESGDLPASRLQSLRYVSTSGQAFPGKYIRLLR
                     RALPAVLIYCNYGSTECKRIAYLPPAELDRRIGSVGKAIPGIRTYLVGNDNALVVRPWE
                     VGELAVAGDLLMEKYWGRESDTARVIREGCFGESRVFMTGDLFAMDEDGFLFFQCRKGD
                     LF"
     CDS_motif       7179..7238
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JG536_RS25020_0001"
                     /evalue="1.90E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="JG536_RS25020"
                     /protein_end="178"
                     /protein_start="158"
                     /score="23.4"
                     /tool="antismash"
                     /translation="ATIFYTSGSTGDSKGVLVTH"
     CDS_motif       7755..7835
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JG536_RS25020_0002"
                     /evalue="9.70E-06"
                     /label="NRPS-A_a6"
                     /locus_tag="JG536_RS25020"
                     /protein_end="377"
                     /protein_start="350"
                     /score="18.0"
                     /tool="antismash"
                     /translation="PWEVGELAVAGDLLMEKYWGRESDTAR"
     CDS_motif       8025..8075
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_JG536_RS25020_0003"
                     /evalue="5.20E+01"
                     /label="NRPS-A_a3"
                     /locus_tag="JG536_RS25020"
                     /protein_end="457"
                     /protein_start="440"
                     /score="-2.1"
                     /tool="antismash"
                     /translation="VVPVSRSDGARVPKAYV"
     gene            8123..9262
                     /locus_tag="JG536_RS25025"
                     /old_locus_tag="JG536_25025"
     CDS             8123..9262
                     /EC_number="2.1.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659960.1"
                     /locus_tag="JG536_RS25025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_25025"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_233410201.1"
                     /transl_table=11
                     /translation="MSPPSGFRRGSGKHRLPAGAAKNVRRQGVRPGFELIPHATRSTMT
                     SSGIEDGAEQYLESAPMIRMASYFHNLATGATQVPKQSVASRIRHLLRDPDVLAYHDLM
                     VAEAGPLFTHFLASVPAILEEMSRVGVALTRYSERRRQHADQHFEFYEVDAFDGTNGRT
                     LAKRSNGLIRTFTNSPNPANETAFNQFANHDFSTFHPQAFAHVTPRLFREHARLDRFSG
                     TFDYIYETAAFQFYGVDRADQIRHVARLLKPNGLIFFLEKLNCDSLDEYDKRESVKDEL
                     HKSHYFSREEIDWKRNQMLSQMQNGQVTFDTLVASIHEQFEHVYLLWNGTNFHEFVASN
                     DARSIEEFVSLLGEPCIEPVFCFDHPVPRRVAGPRGDAT"
ORIGIN
        1 tggaaagggc gtcagctgcg ccgggccggt cgtcgatcgc gtatccagcc tgctcagcgt
       61 cgccggcgtc gatgccgcga cgaagcgcgg cgtactcacg tatgcggcgc cgcgtgcatc
      121 gaaatgaagc ccggcaatcg gtgcattgac cgagcctgcg aacggcgcgg gatccccacc
      181 gcctgtcgga gtctcccaac ttactccggc atagcttcgc cagcgctcga gccggatatc
      241 ggccgccgca gtcgctgcag tcgcgcaact gatgaaggca aaggcgaacg caattgccgt
      301 aactcgattc gagcgcttga acatgatgag gatttcagtg agtcggatgg ggttatgcga
      361 cttggcgcag atgcagcaac tggaagtttt cgatccaccg accggcgctc ccgcggaatg
      421 cgtccatcag cgggctacgc atgtgatgct cccaggacgc acggtccagc cattgctcga
      481 agaagaccac cgtcccgggc tgctccagcg attcatgcag ttcatagctg agacagccgg
      541 gctgatggcg cgccatttcg accagttccc gctgcgcttg caccagcacc gcctcataac
      601 cttgcttcgc ttcgacgagt gcgacgatga catgggcgga catatcgacg ctcccgttca
      661 gagaatcgac cggaaggtgc cgccttcgac gcgcaccgcc gcgccgttgg acgccgcgcc
      721 caacggactg gccagcaacg ccaccatggc ggcgatctcg tcggcttcga tcatgcgggc
      781 gatcagcgag ctcgggcgat aggtctcgaa gaagtgcgat tcgatttccg catcgctcat
      841 gccggggcgc ggcgcggtct gccgcaaata cgcgacgatg ccttcggaac gcgtggcgga
      901 cggcagcacg ctgttgacgg tcacgcccgt gcccttcgtc agttcggcca tgccgcgcga
      961 aaccgcgagc tgtgccgatt tgctgacgcc gtagtgcacc atgtccggcg gcgtaaacgc
     1021 gccgacctcg ctggacatga agatcacacg cccccagttc ttcgcaagca tgcgaggaaa
     1081 gtaatggcgc gacagccgca cccccgacat cacgttgacg tcgatcattc gcagccagtc
     1141 gtcatcggtg atctctgcga acggcttggc ttcgtagtag ccgagattgt tcacgagaat
     1201 atccgtatcc ggctgcgcgt cgatcagttc ggcaacgcct cgcgggccac tcacgtcggc
     1261 aacgattccg cgtacgctgc cttccgggac tgccgcggta atttgagcga cggcttcgtc
     1321 gagtttgtcg cggtgacgcc ccgtgatcgt gaccgacacg ccctcgccga ccaacgttcg
     1381 cgcgatagcc atgccgatgc cggcggtagc gcccgtgacg atcgcggttt tgccttgcag
     1441 ttgcagatcc atgtgttcgt tctcctttgc atcatcgaag aaccggcgat gccggtccgt
     1501 tgcgtggcgt tacttcagcc gcttcagcat ttcgccggcg acgtcgagtg cggacgccgg
     1561 gttctggccg gtgatcaatt cgcgatcggt caccatgtga gcggtaaagg gcacctcgtt
     1621 gctgctgtaa attccaccgg cgcgttgcaa cgccgtctgc ggatagaact tcatcgcgcc
     1681 gccgccgagc agtgctttcg cctgctcttc ttccgcgttg ctgatcaccg tcattcggta
     1741 gccggcatag atccatttcg gcgcgccatc gccggccttc ccggccgcga gttgccgtgt
     1801 aaagccctgt gcatcgggca gcgtcgacag cagcgcaatc ggcccatgac aaacgagcgc
     1861 cgtggtcttg ccgcgcgcat ggaaatcggc cagcagcctg cccactgcgg ggctagtcag
     1921 aagatcctgc atcggcgcat gaccgccggg gacataaacg gcatcgaagt ggccgtaacc
     1981 gatctgctcg acccgcgcca gactgatcac gggagagtgc gcacgcgacg tgatctgcag
     2041 cttgtcgagc aaagcccggt agtcgcgcat cgccttttcg tcgccgttga agtacatctt
     2101 gtcggcagac gtggcatcca tcgtcggcgc cttgccctcg ggcgtggcga aggtcacctg
     2161 atggcctgca tcaagcaggg ccttcacggg ctgcatcagt tcgttcaggt agaagcccgt
     2221 cgcgaacacc ttgccgtcct tcagatcgag gtgatcggaa tccgacaaca ccaccagcac
     2281 gttggcggcg tgagcgctgt acgcggcaat gccgagcatc gcggctacgg cgagcttgcg
     2341 catcgaatac atggttttct ccatcgggga atgtcaggcc tgttcggatc ggttcagcaa
     2401 caggtagcga atcgccgcgc ccacaccggc agccgactgc gggttctggc cggtcacgag
     2461 acgcccgtcg accacgacct tcgccgtcca gctcggtgcc ggatggtgat gcgcacctcg
     2521 ctgcgtcaac gtgctcgcaa gcaggaaagg cacgacgtgg tcgagctgga ccgcgcgttc
     2581 ctcttcatcc gtgaatgcgc tcaggttctt gccggcgacc aggtaagtac cgtcgccgag
     2641 cgtcacatcg acgagcgccg cggggccatg acataccgct ccgaccacgc cgccagcctc
     2701 gtagaccgcg cgcgtcacct tttgcacgtc tgcattgccc ggaaaatccc acatcgcacc
     2761 gtggccgccc gcaaagaaga cggcggcgta tttcgacgca tcgacatcgc ccagtcgcag
     2821 cgtgtggcgc agcgcgtcgc ggaacgcact gtcgttccag tagcgcgcgt tgacttcatc
     2881 cgtcagatcg agaccatcga caggcggctc gccgccctga atcgacgcga attcgaccgg
     2941 gatgccggcc gcgtcgagtt cggccagcgg atgcgtcact tcgccgaggt aaaaaccggt
     3001 cggctcgccg gtcgcgcctt tcgtcgcgtg gctcgtgagg acgaacagca cggtgaggcc
     3061 ctgccggtcg cgagggtcac gattcatggt ttgctccttg atttcggtga gtgaccggac
     3121 gacgcagtca cgatgcgctc actttatttg gcccattcaa ggagataaac tggcttaaaa
     3181 gtaaatcttc tgttctttgg aggacaagat ggcgcgaaga ttcgaccatc tcggtgacgt
     3241 ggaagcattc gtcacggtga tcgaaaaagg ctcgatgaca gcgggcgcgg tggcgctggg
     3301 cagcacaccg tccgttctca gccgtgccat tacacgtctg gagagccggc tgggcgtgca
     3361 attgctgcgc cgttcgactc gacggttgag cctgacggag caaggcaggc tctatctgga
     3421 gcaatcgcgg gccgcgttct tgctgatcga cgacgccgag cgtgccattc aggggcacgg
     3481 cggcacgttg acaggccatg tccgcctcag cgtaccgacg acgtacggac actatcgtct
     3541 gccgccgctg ctgcggcagt tcacgcagca gcacccggag gttcgcgtcg agttgaacat
     3601 cgcgaaccgg aacgtggatc tggtggccga agggttcgac ctggcgatcc ggctaggtga
     3661 gttgccggac agcggcctgg tcgcacgcaa gctcgaggac gcggcgctgt gccttgtcgc
     3721 gtcgccggag tacgtggcgc gcgcgggcat gccggaaaac gtggcggatc tggcgcacca
     3781 tctctgtttg cctttcatgc tgcccagcag cggccgtatc gcgccctggg tgttccggga
     3841 cggcgggcag gacatcgatt ggctcccgcc gtcgaacgtc gcggtctccg acgacgtgct
     3901 gggggtcgtt tcgatggcgc agagcggcgc cggcatctgc cagacctacg acttcattgt
     3961 tcaggatcgc gtgcgggccg ggagtctcgt cgaagtgctg cctcatcttc gtgggcgcac
     4021 acgggcgttt tcggtgatct atgcgccgca tcgtcggttg tctgcggcgt cacgggcttt
     4081 gattgacttg atcgttgcgt ctactggcga tcagtgaagg gaaaacagaa gggttgggga
     4141 tagactcctg tgggcagaga gtgtcgcgcc gatcgtcgcg gcgcgacgtg gggccgcgcg
     4201 ctcgccgaga agcgacggta cccgtctgga tacgtcgcag ttcggtcgac tttcgcggga
     4261 gcatcgactg taagcagata ctggtcggcc aaggatttcg tttgaaccca aagatttccg
     4321 cgccgttgcc ttcatgacgc gtcttctcgc atcgcttgcc aaccaagctt gcttacatgg
     4381 caagccggat tcgctgcgcg atgatgcagg cagcgcaaat ggttcggccg ggattcatgg
     4441 gcatctgaag ccttggatat tttcaagcag tgcgaataaa tcacacttga ggcagctacg
     4501 gtctggagcc gaatgataaa agaagagaac gatagtcggc cccgcaggga acatcagttg
     4561 tcggcgccct tgtattccat tcgatgccaa tcgctggagg aactccgagt tggcctcggc
     4621 cggccgtgcc cacacgagaa cgtcttggta cgcaccggag atcgctctgc ttgttcgcta
     4681 cgtcgcgatc gcaaggcagt gtcgctgcca gggaaacacc gccactgatt atgtcggcaa
     4741 gccaggcaat ccacaaaaaa tgtagataaa ctacatcacg cctgtccagc cttaccccta
     4801 catctccaag gtagatttac ataatcaccg gaaattcccc catattccgc ccttgaccag
     4861 ccacgccatc agcggttaat gttgaaaaac tacatgattt agccggccat tctgttacgg
     4921 gcactggctt gctttttcgt ggcttcgctt ccgctgagag caggacaata atttcttaca
     4981 aaaaagaaag gagtgcgaat atgttcgtca ggaacctgag agacgtcgaa aaggatggcc
     5041 tgcttgtcga atggggctcc ggcacaagcc atcgcttgtt gacggaaaag gacggcatgg
     5101 gctacggcgt gtgccatacg gtggtgcgtg cgggtacaga atcgctcctg caataccgca
     5161 accatctcga agcctgctat tgcattgcag gcgaaggcga ggtcgaggac atgaacggta
     5221 acgtctttcc gatccgcccg ggcaccatct acgtgctcga tcgacacgac cagcattacc
     5281 tgcgcggcgg caaaaacggg gatctggtgc ttgtcagcgt cttcaatccc ccgctcaagg
     5341 gacacgaacg gcataacctg gggtctgcag aagcgtccgc ttactgacgc tcgatacgct
     5401 cattccggga ggccctgggt accagccctc ccggcaaccg tccgcaggag cgcgcatgca
     5461 tcgcgtgctc cgcgcaaaag aaaggtgact gttcgtgcgc gcccagcatc aagccttttt
     5521 ccattcgctt ttccttgccc gtctcgccga ccagattctg ttgtttctcg tgccgctggt
     5581 catctttcag atgacaggac gcgtgtcctg gtcgggcatc gcgttttcgc tcgaaacact
     5641 gccgagatac ctgtgcttcc cgtttctagg tgcgctttgc gaccggtatt cgcccacgaa
     5701 actgatgcgc gtcagtcagg ttctccgcgc ggcagtatgc gtcgcgggcg tcatcgccta
     5761 cgacctcgtc ggcggtatcg gatggctgat cgcgctgtcc gcgttgtgcg gcatgctgac
     5821 gagtcagggc atcgttgcgc gcgaggtgat gctgccgcag atattcagca gccatcgctt
     5881 cgaaaaagtg ttgtcgtatt cgcaattggc cgaccagctt ggtctggtgc tcggccccat
     5941 gctcgcggcg atcctgctgt cgtggtggcg ctgggaattc gtcgtggtgg ccgcggccgt
     6001 gctgtttgtc acagccgacg tcgcgctcgc ctattggcaa aaggccagcg cgttcgaatt
     6061 cgtgatgccg gaaggcacgc acggcacctg gttcacgccg atcaagacgg ccttgtctca
     6121 cgtcatccgt ctccctggcc tcaagtccgt catcatgttg gccgccgcgg agaacctcgt
     6181 gatcggcgtc acgctcgcga catccgccgc gatggtcacc ggctttcatc acgaaagcgg
     6241 acgctattac gcaggactgc aaacggcagg cgcgtgcgtg acgatcgcga ttctgctgat
     6301 gacggcgagg ttgcatgtcc cgaggcgcgt cctcggcctc gtcggcttcg gttcgatctg
     6361 tatcggcggc gttatcgcgg cagccagccc gggaattgcg gggtacgttt tcggcttcct
     6421 cctgatcgtc ggattcgaca agatgttcaa cgtgtacatc cggagcacgc ggcagaagat
     6481 catccccgcg cgcgactacg gcaagacgac gggagtcgtc atcctcctca acaacatgac
     6541 acagccgctc gcgggcgtcg cggtaggcgc gttctcgacg cgtttcggac cagacacggt
     6601 aatcatggtg ctgtccgtgt gcatgggttg cctcgggttg ctggtctcgc tcgcgccccg
     6661 cattcgaaaa ggccaccgcc cctcgtcggc ctcggtgtcc tcacatgaag gctgatgcca
     6721 tgattgaacg attcccgcaa tacttcgagc gcagcgcgag cagccggcga gacaaagtcg
     6781 cgctggtggt cgacggacgt tcggcaacct acggcgaatt gctggacgtg atgttgacga
     6841 tagccgatac gcttcaccgt ctcggcattc ccgccggcgc gcgcgtcgcg atctattcgg
     6901 agatgtccgt gcatgcggtg gcggccgcat tcggcatcct gaaggccgga tgcgtactgg
     6961 catgcatcag gcacacgatc gacaccgccg aactggagca gcagatcgcg gattgcggcg
     7021 ccagcgcgct cgtggcatcg cgagcgacct cgccccgcat cgcgtcccgt tatcgcgcgg
     7081 tcacgatcga cgcgacccgc ggcgacctgt cggaatatgt cgtctgcttc gcggccgacg
     7141 gcgcatccga agcgggcaat tcctccatgg atgacgcagc aacgatcttc tacacatccg
     7201 gctcgacggg cgattcgaag ggtgtgctcg tcacgcaccg gatcatgatt gccgcgttcc
     7261 gctcggtctc cggctatctc gccaatagcg aacgcgacat cgtgctcagt ttctccccgg
     7321 gcttgagctc ggactacggc ttttacaaca cggtcatgcc gctgctgttc gggggccgtg
     7381 ccgtggtcga gacggcgttg cccgcttcgg ccgccgccat cgtcgacatc atcgagcgac
     7441 acggcgtaac cggcctgcac gtctttccgc cggcgctttt ccgtctgtgc gaatcgggcg
     7501 acctgcctgc ctcgagactg caatcgctgc ggtatgtctc gaccagcgga caggcgtttc
     7561 ccggcaagta cattcgcctg ctcaggcgtg cgctaccggc tgtcctgatc tactgcaact
     7621 acggttcgac cgagtgcaaa cgcatcgcct acctgccgcc cgccgaactc gatcgacgta
     7681 tcggctcggt cggcaaagcc attccgggca tcagaaccta cctcgtcggc aacgacaatg
     7741 cactcgtggt ccggccctgg gaggtcggtg aactggcggt agccggcgat ctgctgatgg
     7801 agaaatactg ggggcgcgaa agcgataccg cccgcgtcat tcgcgaagga tgtttcggtg
     7861 aatcgcgcgt gttcatgacc ggtgatctgt tcgcgatgga cgaggacgga ttcctgttct
     7921 ttcaatgcag gaagggcgac ctgttcgatc gagacggcgt gccggtgaat ccgcgcgccg
     7981 tcgagcgggt attgctcgac cacgaaaccg ttgcggaagc gctcgtcgta ccggtttccc
     8041 ggtcagacgg cgcacgcgtc cccaaagcct atgtcgttcc ggctgcgaca tctgcggcca
     8101 atgcaatcga gctgctcgat cattgtcgcc gccatctgga ttccgccgcg gttccggcaa
     8161 gcatcgactt cctgcgggcg ctgccaagaa cgttcggcgg caaggtgtcc gcccagggtt
     8221 tgaactgata ccacatgcga cgaggtcaac catgacgtca agcggaatcg aggacggcgc
     8281 ggagcaatac ctggaaagcg cgccgatgat ccggatggca agttattttc acaaccttgc
     8341 gacgggcgcg acgcaggtgc cgaagcaatc ggtcgcgagc cgcatccgcc atttgctgcg
     8401 cgatccggac gtgcttgcgt atcacgacct gatggtcgcc gaagccgggc cgctgttcac
     8461 gcattttctt gccagcgtgc cagccatact ggaggaaatg tcgcgcgtcg gcgtcgcgct
     8521 cacgcggtat tccgaacgtc gcaggcagca cgcggatcag cacttcgagt tttacgaagt
     8581 cgatgccttc gacggcacca acggcagaac gctggcaaaa cgatcgaacg gcctgatccg
     8641 cactttcacg aactcgccca atccggccaa tgaaacggcg ttcaaccagt tcgccaatca
     8701 cgacttctcg acgttccacc cgcaggcatt cgcccacgtc acccctcggc tgttccggga
     8761 gcatgcgcgc ctcgaccgct tctccggcac gttcgactac atctacgaga ctgccgcatt
     8821 tcagttctat ggcgtggatc gcgcggacca gattcgtcat gtcgcgcgtc tgctcaagcc
     8881 gaacggattg atcttcttcc tcgagaagct caactgcgat tcgctcgacg agtacgacaa
     8941 gcgcgagagc gtcaaggacg aactgcacaa gagtcattac ttcagccgcg aggaaatcga
     9001 ctggaagcgc aaccagatgc tttcgcagat gcaaaacgga caggtgacgt tcgatacgct
     9061 cgtcgcgtcg atccacgagc agttcgaaca tgtctatctg ctctggaacg ggacgaattt
     9121 tcacgagttc gtcgcctcca acgacgcacg atcgatcgag gaattcgtct cgctgctcgg
     9181 cgaaccctgc atcgagcccg tgttctgctt cgatcacccc gtaccgcgca gagtggccgg
     9241 gccgcgagga gacgcgacgt gatgcagctg catgatgacc tcccgtcaat ggatacgatc
     9301 cggtcatgcg tggacgggct cgaactcgag cgcacacaga tcggccgacg catcgaacaa
     9361 ctgctcccgc gctggcaggc tcgcgcacag gcggccgatc agttcgcgcg cctgtcgccc
     9421 gagacaatgg ccgaactgca cgacgccggg ctcctccacc tgctgacacc gcgccgctac
     9481 ggcggattgc aacaggactt tccgacactc gtcgaagctg cgcggctcgc ggccaccgcg
     9541 tgcgcgtcca cggcctggat gttaagtgtc gtcggcggac atgtgacgac tgcggcgcgc
     9601 ctgcccgccg cctgtcagga catcatcttc gcgaacgggc cgcgacagct gttcgcgacg
     9661 gccacggttc agaacggcac gttcgtcagg gacggcagcg gattccggtt gaacggcatc
     9721 tggcgctaca gctcgggcat cgatcacgca acctgggtga tcgtctcggc acgactcagc
     9781 gggacggacg caccggaggc ccaggagatc ttcaaggtcg tggttcgacg cgaagacgtc
     9841 cgtgcgctcg acacatggca cgtatccggc atgcgcgcga caggttccag ggatattgca
     9901 ttcgacaatc tgtacgtatc ggacgagtgg atcttcgctc gttcgaatta ttttggcccg
     9961 catcccgccg gcgccgacct gcatcccgac gcgtatctgt acgacgtggc gctcatcccg
    10021 tacagcacga cgtggatcat cggaccgatt ctaggctgcg cacagggcgc gtaccggcac
    10081 tgcatcaata cgctccgcag ccgcggtaga agctgcgacg cgatgctttg cgggcacatc
    10141 gcgaaaagcg acgcggagct cacctgcgcg ggtcgtctct acgacgcgct ggtccgcacc
    10201 ttgcacatgg ccggcatcgc gcggcgcgcg gtgtcgcccg ccgagacggt ccgcatcagg
    10261 cgtgatcgcg cattcatcgc gcagctttgc gtcgacgcga tccgacgact gatcggcagc
    10321 atgggcaccg ccgtggcatt cgacgcccat cctgcacaac gtcactggcg cgacctgcag
    10381 gtcatgg
//
