LOCUS       NZ_CP066763            10399 bp    DNA     circular CON 30-NOV-2024
DEFINITION  Burkholderia ambifaria strain BJQ0010 chromosome 2, complete
            sequence.
ACCESSION   NZ_CP066763
VERSION     NZ_CP066763.1
KEYWORDS    RefSeq.
SOURCE      Burkholderia ambifaria
  ORGANISM  Burkholderia ambifaria
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales; Burkholderiaceae; Burkholderia; Burkholderia
            cepacia complex.
REFERENCE   1  (bases 1 to 2704441)
  AUTHORS   Xu,Y.
  TITLE     Complete genome sequence of Burkholderia ambifaria BJQ0010
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2704441)
  AUTHORS   Xu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2020) School of Food and Chemical Engineering,
            Beijing Technology and Business University, No. 11, Fucheng Road,
            Haidian District, Beijing 100048, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016458785.1-RS_2024_11_30
            Annotation Date                   :: 11/30/2024 03:04:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,675
            Genes (coding)                    :: 5,537
            CDSs (with protein)               :: 5,537
            Genes (RNA)                       :: 85
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 88 of 138
            Pseudo Genes (incomplete)         :: 78 of 138
            Pseudo Genes (internal stop)      :: 12 of 138
            Pseudo Genes (multiple problems)  :: 34 of 138
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:03:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 591212
            Orig. end                         :: 601611
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066763.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(289..303)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.87"
                     /tool="antismash"
     misc_feature    complement(327..338)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    526..542
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.23"
                     /tool="antismash"
     gene            complement(1003..1140)
                     /locus_tag="JG536_RS18750"
                     /old_locus_tag="JG536_18750"
     CDS             complement(1003..1140)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JG536_RS18750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JG536_18750"
                     /product="hypothetical protein"
                     /protein_id="WP_158380540.1"
                     /transl_table=11
                     /translation="MAKLLGYSAPALKRRNASRGPPYAAFRDFPPKIVEQFRPILTSSN
                     "
     gene            1139..1981
                     /locus_tag="JG536_RS18755"
                     /old_locus_tag="JG536_18755"
     CDS             1139..1981
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366217.1"
                     /locus_tag="JG536_RS18755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_18755"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_175667547.1"
                     /transl_table=11
                     /translation="MSARFPLRHFLRGALVPFAFACSLAAAAPLQTVRPGTLLVGSDLT
                     YPPYAYLEAGKPAGFDAEFARLLARHLKLEPVFLDTRFPDLILGMKARRFDVVASALYV
                     TPDRVKQVDFVPYLKTGASLLVAKGSAFQPKTPQDLCGKRVGSIKGASWTPKLRAVSQD
                     TCKAAGHGEIAILEFPTSPEATSALMSKAVDVQIEDAAVAQGIPKQTNGRVDVSSTTLL
                     YPIVIGLGMTKGDSGLQGALERALNDAKRSGEYGSLVKKYGLQEPTPADVANALGTAK"
     gene            2068..3570
                     /locus_tag="JG536_RS18760"
                     /old_locus_tag="JG536_18760"
     CDS             2068..3570
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396887.1"
                     /locus_tag="JG536_RS18760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_18760"
                     /product="amino acid ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="WP_175667548.1"
                     /transl_table=11
                     /translation="MQFDWHYAFQLLWDKDFWRACVVVVELSVVAWLLGVLLGFPVALA
                     RQSHRPLLKRAAAVYIWFFRSLPLLVLLVFIYNLPQVFPATSAVLSDPFTAGLIAMVLS
                     ETAYFAEIHRGGILSVPRGQLEAGRALGIRFAGIQRLIVMPQAIRIALPALANEFITIV
                     KLTSLVSVISLAEILLVGQRLYTQNFLVFETMLAVAFYYVLIVTVFSRLLGYLERHLDV
                     SRRTPVQADDIDLALPPSAPAVAPRQRANAEFALRLKGVHQRYGRHEVLKDIHLDVVPG
                     QVVSIIGPSGSGKTSLIRTINGLAPVNAGEIDLYGEPFIRASGSHPPRVSHERILDIGM
                     VFQSFNLFPHRTALQNVMMAPCYHRQADAAAIRLQALSLLSKVGMLEHANKYPHQLSGG
                     QQQRVAIARALATRPSIMLFDEPTSALDPELVGEVLKVVETLAKEGMTMIIVTHEMNFA
                     FKVSDRIVFMESGRILYDVPPERIRGSGDARVKDFLKHVHIA"
     gene            3594..4934
                     /locus_tag="JG536_RS18765"
                     /old_locus_tag="JG536_18765"
     CDS             3594..4934
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1105: amidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658988.1"
                     /locus_tag="JG536_RS18765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_18765"
                     /product="amidase family protein"
                     /protein_id="WP_175668368.1"
                     /transl_table=11
                     /translation="MDPMGFRLGDMNARSCVERLSASLARAEAYPGDIGTAWLARRDAA
                     ALADARAWDAALRGGGVDAPLAGAVLTVKACFDCEGWVTHAGSRVLADEPPARTDAAVV
                     SMLRRAGAVLVAQTAMTEFAYGALGVNRAYGTPTTPLDARRERVAGGSSSGAAVSVALG
                     AADLSLGSDTSGSARIPAAFCGVTGFKPSRGRYASDGMRFLSTSFDVPGLLAATVELCR
                     QADVALHGRDARRRRPATIRDLQFVVPEPFATDDVDPVVGRAFDAWLSRLSAHGACIRR
                     TRLDCVAEAGAVARAAGIIAAEAFMLHRARLATAADRYDPLVGARIAAGEQVRAHDYAA
                     ALLRLTALADTYHAELGDADAVLTPTVPMLPPRVADLANEAAYLEQNARAFRLTEFANR
                     LDLPSISVPGDLHNRQPVGLLITGRRREDARLLDVAAMVERALTDST"
     misc_feature    3620..3631
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     gene            5001..5399
                     /locus_tag="JG536_RS18770"
                     /old_locus_tag="JG536_18770"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756328.1"
                     /locus_tag="JG536_RS18770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_18770"
                     /product="ectoine synthase"
                     /protein_id="WP_175667549.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.8e-39, bitscore:
                     130.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVVKSTDIANTERHARGPGWDSKRMIVKQDGVGYSVHETRVREG
                     AELHLHYKNHFETNYCVAGEGEVVDTATGDVHRITPGTIYALNLNDPHILRATRGDLHL
                     VCVFNPPLTGLETHREDGSYALPTEQQA"
     gene            complement(5452..6126)
                     /locus_tag="JG536_RS18775"
                     /old_locus_tag="JG536_18775"
     CDS             complement(5452..6126)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216888.1"
                     /locus_tag="JG536_RS18775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_18775"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_105831181.1"
                     /transl_table=11
                     /translation="MASEKARGQSVADRISHQLREHIISGKLPPGTALIEMDLAAEYDT
                     SRNSLREVLHQLGREGLVTFVRHKGVVVRTLNRQDVRDLYVARRTLELHALNTAELAQP
                     ALLEKMQSAIRAAERELAKENWQAVGTHSLRFHQHIVALQKSALLDEFFRTISAQLRLV
                     FAADPDEKRVQTPDWIQREYQIYDLLTQNRRSEAVTALARYLDDSERMLVDAIKRSHRE
                     AA"
     gene            complement(6212..7072)
                     /locus_tag="JG536_RS18780"
                     /old_locus_tag="JG536_18780"
     CDS             complement(6212..7072)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216889.1"
                     /locus_tag="JG536_RS18780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_18780"
                     /product="DUF1989 domain-containing protein"
                     /protein_id="WP_175667550.1"
                     /transl_table=11
                     /translation="MSNYPAAYQVTQGSVLNVDKAFYSAIARRHDARERIASHVVPIRS
                     GFAWTVPAGHVFRIVTLEGPQVADFNMWNLHNPRERMWASRTRQLQAAHVTTFDRLWST
                     LPYLRPMATITDDTLAGYGVDGDGGRVHDLLGTRCDPYVNKLLTGEDFHFHCHSNLVRA
                     VAPYGLTEFDVHDVLNVFQCTGLNDDDKYFMKACPAQPGDYLEFFAEIDVLCAMSTCPG
                     GDLSVPMWGPDARDPIDVCRPLGVEVYRLDPSLLEGWASPPVAPYRGLHGMQPPHADWQ
                     HDKTA"
     misc_feature    7162..7175
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.38"
                     /tool="antismash"
     gene            complement(7365..7901)
                     /locus_tag="JG536_RS18785"
                     /old_locus_tag="JG536_18785"
     CDS             complement(7365..7901)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488701.1"
                     /locus_tag="JG536_RS18785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_18785"
                     /product="hypothetical protein"
                     /protein_id="WP_175667551.1"
                     /transl_table=11
                     /translation="MRFRSYMAAGTLALMPVLALAGQADAAPAAQPMFVNVNGQAVPVK
                     TDTRVVQTAVGPMQVRTWSWHSPHGGASFEMQTSSSGSMPPTAARQMQAAQYQMQAAQA
                     QMVAMQQQMMALQHVALANAFAMPMPQPVGFAMPMWAMPQPVVVIVPARPSTRALAPAP
                     STPARPAARGPEIKA"
     gene            8259..9506
                     /locus_tag="JG536_RS18790"
                     /old_locus_tag="JG536_18790"
     CDS             8259..9506
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756332.1"
                     /locus_tag="JG536_RS18790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_18790"
                     /product="multidrug effflux MFS transporter"
                     /protein_id="WP_175667552.1"
                     /transl_table=11
                     /translation="MNASLTPARESNSPRFLLFLICLFSSAGQLAIDIYVPALPDMARS
                     FATTPQAIQSSVSGYMAAYALGQLIFGPVADAYGRKRVLAFGLVIYTIGCLLSLGAPNL
                     ETFILARCLQGFGIATTNLLAKAIITDSFSGQALMHAFTYMSIAWGLAPIIAPVIGAHL
                     QEWFGWRACLVFLLVYSLVMWALWWHYRETLPKPVHLEPRTLIANAGKVLGSPVFQSCF
                     LAQGLCYSILLVFNIVGPFMVQTTLHKPPTFFGYLALGIGLMYFLGGLSNRIHGRGLPS
                     AEQRLRIGARVMAVAAVVMLVLALTVGLRVWTLATPVLVMGLCAGAMYPTLMAKGNSLF
                     PHIAGLTSAILGCALLLVSSAMMGLAGFVSVQVLTPLAVFFVALSFIVVWMVTKLLRYL
                     TQQQAASVACGSGEAA"
     gene            complement(9542..10234)
                     /locus_tag="JG536_RS18795"
                     /old_locus_tag="JG536_18795"
     CDS             complement(9542..10234)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366209.1"
                     /locus_tag="JG536_RS18795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JG536_18795"
                     /product="hypothetical protein"
                     /protein_id="WP_175667553.1"
                     /transl_table=11
                     /translation="MNIRPPHFTRPALGALCVAALATLAGCNGEACFGVDACFNDNNTQ
                     TVALSGTAATGGALASASVTVSCAQGSATTLTDGGGNYRVTVNAVLPCVISVASGGTSL
                     HSLAYAGGTFNTTPETELMLVYLAAQLGTNTAGLIGNFQGSRRFQQVMGDPGTVQAAQS
                     AVVTNLQQRYSVTLATPAFLTTPFVVGQPGVDADLGALAKAGAIDSNGMPDPVAVSLLT
                     QAGAAHPL"
     misc_feature    10365..10376
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.1"
                     /tool="antismash"
ORIGIN
        1 tgaaggcgcc gaacctgacg gtgaaggaaa tcccaattac gtcgcagaac cggattccgc
       61 tgctgcagaa cggcacgatc gacttcgagt gcgggtcgac gaccaacacg ctggagcggc
      121 agaagcaggc cgcgttctcg aatagcatct tcctgtacgg gatccgcttc agcacgcgca
      181 aggattcggg cgtgaaggac ttcgcggacc tggccggcaa gacggtcgcg acgacggccg
      241 gcacgtcgga cgagcgcctg ctgcgcaagc tcaacgagga gaagggcatg aacatgacga
      301 tcatcagcgc gaaggaccat gccgaagcgt tcatgaacgt cacgacgggg cgcgcggtcg
      361 cgttcgtgat ggacgagccg ctgctgtacg gcgagatcgc gaaggaccgc aacccgggcg
      421 catacacggt agcgggcacg ccgctcgtcc atgaaaacta cgcctgcatg atgcgcaagg
      481 atgaccctgc gttcaagcac gtggtcgacg gcgtgatcgc gaagctgcag acttccggcg
      541 cggccgagaa gctctacgac cagtggttca tgcagccgat tccgccgaag ggcgtgagcc
      601 tcaactatcc gctgtcgccg gagatgaagc agctgttcag gaatccgaca gatcaggcgc
      661 agtactgagc agaacgtcga gttggcgtgc gggccgacac gtagcgtgct cggccctgca
      721 cgatcgaatc tgtccggcct gcgggccggc gtcgcggtgc gcatcctgcg gccgtagtag
      781 ggcccgtgcg catcgcgccg gatgctgaaa cccgccctct gatccctcac cgcacgcgtc
      841 gcgcgtgccg tgccgcactt cgaagggcct cgatccgggg cccgtctcca ccggtctttt
      901 ccctccttcc ccaaacgcat gctcgtgccg acgcgtggtg cgcgtgtgcg tgccgactcg
      961 cgtccggtga tgtcgtctgc cgcgtaaacc cgaattcaga tttcaatttg aactggtcag
     1021 aatcggccga aattgttcaa caattttcgg cggaaaatcc cggaaagccg catagggcgg
     1081 cccgcgggag gcgttgcgac gtttcagcgc gggagccgaa taacccagga gcttcgccat
     1141 gtccgcccgt ttcccgcttc gacatttcct ccgcggcgcg ctcgtgccgt tcgcattcgc
     1201 gtgctcgctc gcggccgcgg cgccgctgca gacggtgcgg cccggcacct tgctggtggg
     1261 ctccgacctc acgtatccgc cttacgcgta cctggaagcc ggcaagcccg ccggcttcga
     1321 cgccgaattc gcgcggctgc tcgcacgtca cctgaagctg gagccggtgt tcctcgatac
     1381 gcggtttccg gacctgatcc tcggcatgaa ggcgcgccgt ttcgacgtcg ttgcatcggc
     1441 gctgtacgtg acgcccgatc gcgtgaagca ggtcgatttc gtgccgtatc tgaagacggg
     1501 cgcatcgctg ctcgtcgcga agggcagcgc gttccagccg aagacgccgc aggacctgtg
     1561 cggcaagcgg gtcggctcga tcaagggtgc gtcgtggacg ccgaagctgc gtgcggtgtc
     1621 gcaggacacg tgcaaggcgg ccgggcacgg cgagatcgcg atcctcgagt tcccgacgtc
     1681 gccggaagcg acgtcggcgc tgatgtcgaa ggcggtcgat gtccagatcg aggacgcggc
     1741 cgtcgcgcag gggattccga aacagacgaa cgggcgtgtc gacgtcagtt cgacgacgct
     1801 gttgtatccg atcgtcatcg gcctcggcat gacgaagggc gatagcggcc tgcagggtgc
     1861 gctcgagcgc gcattgaacg atgcgaagcg cagcggcgag tacggcagcc tcgtgaagaa
     1921 atacggattg caggagccga cgcccgccga cgtcgccaat gcgctcggta ctgcgaagta
     1981 ggcacgcagc aaggctgtag caagcacgac ccgaccaccg cgcgcgccgc gcggccgcat
     2041 cttccatccg cggttgagga gtcatcgatg cagttcgatt ggcactatgc gtttcagctc
     2101 ttgtgggaca aggatttctg gcgcgcctgc gtcgtggtcg tcgaactcag cgtcgtcgcg
     2161 tggctgctcg gcgtgttgct cgggtttccg gtggcgctcg cgagacagtc gcaccggccg
     2221 ctgctgaagc gggcggcggc cgtctacatc tggttcttcc gcagcctgcc gctgctcgtg
     2281 ctgctggtct tcatctacaa cctgccgcag gtctttcccg caaccagcgc ggtgctgtcc
     2341 gatccgttca cggccgggct gatcgcgatg gtgctgagcg agaccgcgta tttcgcggaa
     2401 atccatcgcg gcggaatcct gtcggtgccg cgcgggcagc tcgaggccgg ccgcgcgctg
     2461 ggcatccgct tcgccggcat ccagcggttg atcgtgatgc cgcaggcgat ccgcatcgcg
     2521 ctgcccgcgc tcgcgaacga gttcatcacg atcgtgaagc tcacgtcgct ggtgtcggtg
     2581 atttcgctgg ccgagattct gctcgtcgga cagcggctgt acacgcagaa tttcctcgtg
     2641 ttcgaaacga tgctggccgt ggcgttctac tacgtgttga tcgtgaccgt gttcagccgg
     2701 ctgctcggct atctcgagcg gcacctcgac gtcagtcggc gcacgcccgt ccaggccgac
     2761 gacatcgatc ttgcgctgcc gccgtcggcg ccggccgtcg cgccgaggca gcgggcgaac
     2821 gcggaattcg cgcttcgcct gaaaggcgtg caccagcgct acggccgcca cgaagtgttg
     2881 aaggatattc atctcgacgt cgtgccgggg caggtcgtgt cgatcatcgg cccgtccggc
     2941 tcgggcaaga cgtcgctgat tcgcaccatc aacgggctcg cgccggtgaa cgcgggcgag
     3001 atcgacctgt acggcgagcc gttcatccgc gcgtcgggtt cgcatccgcc gcgcgtgtcg
     3061 cacgagcgca tcctcgatat cggcatggtg ttccagagct tcaacctgtt cccgcaccgc
     3121 accgcgctgc agaacgtgat gatggcgccg tgctatcacc ggcaggccga tgcggcggcg
     3181 atccggctgc aggcgctgtc gctgctctcg aaggtcggga tgctcgaaca tgcgaacaag
     3241 tatccgcacc agctttccgg tggccagcag cagcgtgtgg ccatcgcgcg cgcactcgcg
     3301 acgcggccgt cgatcatgct gttcgacgag ccgacctccg cgctcgatcc cgaactcgtc
     3361 ggcgaagtat tgaaggtcgt cgagacgctg gcgaaggaag ggatgacgat gatcatcgtg
     3421 acgcacgaaa tgaacttcgc gttcaaggtc tccgaccgga tcgtgttcat ggagagcggg
     3481 cgcatcctgt acgacgtgcc gccggagcgg attcgcggca gcggcgacgc gcgcgtgaag
     3541 gattttctga aacacgttca catcgcatga cgaacgactg gaccggagca cgaatggacc
     3601 cgatgggctt tcggctgggc gacatgaacg cacgctcgtg tgtggagcgg ctgagcgcga
     3661 gtctcgcacg tgccgaagcg tatcccggcg acatcggaac cgcgtggctc gcgcggcgcg
     3721 acgccgcggc gcttgccgac gcgcgtgcat gggatgcagc gctgcgcggc ggcggggtcg
     3781 atgcgccgct ggccggcgcc gtgctgacgg tgaaggcctg tttcgattgc gaaggctggg
     3841 tcacgcatgc cggctcgcgt gtgctggccg atgagccgcc ggcgcggacc gacgccgcgg
     3901 tggtgtcgat gctgcgccgg gccggcgcgg tgctggtcgc gcagacggcg atgaccgagt
     3961 tcgcgtacgg cgcgctcggc gtgaatcgtg catacggtac gccgacgaca ccgctcgatg
     4021 cgcggcgcga gcgggtggcc ggcggatcga gttcgggcgc ggcggtgtcg gtggcgctcg
     4081 gcgcggccga cctgtcgctg ggttccgata cgagcgggtc ggcacgcatt ccggccgcat
     4141 tctgcggagt gaccggcttc aagccgtcgc gtgggcgcta tgcgagcgac ggcatgcggt
     4201 tcctgtcgac gagcttcgac gtgccgggcc tgctggccgc gacggtcgag ctgtgccggc
     4261 aggccgacgt tgcgttgcat ggccgcgatg cgcggcggcg acgaccggcg acgatccgcg
     4321 atctgcagtt cgtcgtgccc gaaccattcg ccaccgacga cgtggatccg gtcgtcggac
     4381 gcgcattcga cgcgtggctg tcccgactgt ccgcgcacgg cgcgtgcatc cggcgcacgc
     4441 ggctcgattg cgtggccgag gccggcgcgg tcgcccgcgc ggccggcatc atcgcggccg
     4501 aggcgttcat gctgcatcgt gcgcggctgg cgacggcggc tgaccgttac gatccgctgg
     4561 tcggcgcgcg catcgccgcg ggcgagcagg tgcgcgcgca cgattacgcg gcggcgctgc
     4621 tgcgtctgac tgcactggcc gacacgtacc acgccgaact cggcgacgcg gacgcggtgc
     4681 tgacgccgac ggtgccgatg ttgccgccgc gcgtggccga tctggccaac gaagccgcgt
     4741 acctcgagca gaacgcgcgt gcgttccggc tgaccgagtt cgcgaaccgg ctcgacctgc
     4801 cgagcatcag cgtgccgggc gacctgcaca accggcaacc cgtcggcttg ctgatcaccg
     4861 ggcgtcggcg cgaagacgcg cggctgctcg atgtcgcggc gatggtcgag cgcgcgctga
     4921 ccgattccac atgacaggtt tcccgcggca gggatcgcct tgccattttc accattactc
     4981 gttactcatc agaggtgctt atgatcgtcg tgaaatcgac cgatatcgcc aataccgaac
     5041 gccacgcgcg cggcccgggc tgggacagca agcgcatgat cgtgaagcag gacggcgtcg
     5101 gctattccgt ccacgagacg cgtgtgcgtg aaggcgcgga actgcatctg cactacaaga
     5161 atcacttcga gaccaactac tgcgtcgctg gcgaagggga agtcgtcgat acggccaccg
     5221 gcgacgtgca ccggatcacg cccggcacga tctatgcgct gaacctgaac gatccgcata
     5281 tcctgcgcgc gacgcgcggc gacctgcatc tcgtatgcgt gttcaacccg ccgttgacgg
     5341 ggctcgagac acatcgggaa gacggcagtt atgcgctgcc gacggaacag caagcgtgac
     5401 gggccgttgc gcgccgcatc atgcggcgcg ataggggcac cgtgttcggg ttcaggccgc
     5461 ctcgcggtgg ctgcgcttga tcgcgtcgac gagcatccgc tcggaatcgt cgagatagcg
     5521 cgcgagcgcg gtgacagctt cgctgcgacg gttctgcgtg agcaggtcgt agatctggta
     5581 ttcgcgctga atccagtcgg gcgtttgcac gcgcttctcg tcgggatcgg cggcgaagac
     5641 gaggcgcagc tgtgccgaga tggtgcggaa gaactcgtcg agcagcgccg acttctgtag
     5701 cgcgacgatg tgctggtgga atcgcaggct gtgcgtgccg acggcctgcc agttttcctt
     5761 ggcgagctcg cgttcggccg cgcgaatcgc gctctgcatt ttctcgagca gcgccggctg
     5821 cgcgagttcc gccgtgttca acgcatgcag ttcgagcgtg cggcgcgcga cgtagagatc
     5881 gcgcacgtcc tggcgattca gcgtgcgcac gaccacgccc ttgtgccgca cgaacgtgac
     5941 gagcccttca cgaccgagct gatgcaggac ttcgcgaagc gagttgcgcg aggtgtcgta
     6001 ttcggccgcg aggtccatct cgatcagcgc ggtgccgggc ggcagcttgc cgctgatgat
     6061 gtgttcgcgc agttgatggc tgatccggtc ggcgaccgac tgtcctcgcg ctttttcgct
     6121 tgccattcct ttcgttcgct cctctcctga tcgtgcgccg ccggcgtggc cggccggcgg
     6181 cgtttcgcca ttctacgggc tatgggcgtc gttacgcggt cttgtcgtgc tgccaatcgg
     6241 cgtgcggcgg ctgcatcccg tgcaggccgc ggtacggcgc gacgggcggc gacgcccagc
     6301 cttcgagcag gctcgggtcg agccggtaca cctcgacgcc gagcggccgg cacacgtcga
     6361 tcgggtcgcg tgcgtcgggc ccccacatcg ggacggacag atcgccgccc ggacacgtcg
     6421 acatcgcgca cagtacatcg atttccgcga agaactcgag gtagtcgccc ggttgcgcgg
     6481 ggcaggcctt catgaagtac ttgtcgtcgt cgttcaggcc cgtgcactgg aatacgttga
     6541 gcacgtcgtg cacgtcgaat tcggtcagcc cgtacggcgc gaccgcgcgc acgaggttcg
     6601 aatgacagtg gaaatgaaaa tcctcgccgg tcagcagctt gttcacgtac gggtcgcagc
     6661 gcgtgccgag caagtcgtgc acgcggccac cgtcgccgtc gacgccgtag cccgcgagtg
     6721 tgtcgtcggt gatcgtggcc atcggccgga ggtacggcaa ggtcgaccag agccggtcga
     6781 acgtcgtcac gtgggcggcc tgcaactggc gcgtgcgcga tgcccacatg cgttcgcgcg
     6841 gattgtgcag gttccacatg ttgaagtcgg cgacttgcgg gccttcgagc gtgacgatcc
     6901 ggaacacgtg accggccggc accgtccatg cgaagccgct gcggatcggc acgacgtgcg
     6961 acgcgatccg ttcgcgcgcg tcgtgacgtc gggcgatcgc ggaataaaag gctttgtcga
     7021 cattcaggac ggaaccctgg gtgacctggt aggcggcggg atagttcgac atgacgcggt
     7081 actctcggga aaggttgtca ggtgcggcga tccggctgta gccctttgtg gtggccgctc
     7141 acgcgtcaat gttcatgcta gattgttcaa caatatattg gcgcttcgcg gagtataggg
     7201 tgaaggcggg gcggtgtgtc aatcggtgga aagcaccgag tgtcggggag gcggcgcgat
     7261 cgcggcggcg cgggcgaacg tccggcgccg ccaatgaaaa aaccgccggc acatgccggc
     7321 ggtttcggtt ttcggcccgg tcgctcgcgc gacggaaccg tgcgtcaggc cttgatttcc
     7381 ggcccgcgtg cggccggccg ggccggtgtc gacggtgccg gcgccagcgc acgcgtggac
     7441 ggccgcgccg gcacgatcac gacgaccggt tgcggcatgg cccacatcgg catcgcgaac
     7501 ccgaccggtt gcggcatcgg catcgcgaac gcgttggcga gcgcgacgtg ctgcagcgcc
     7561 atcatctgct gctgcatggc gaccatttgc gcctgggccg cctgcatctg atactgcgcc
     7621 gcctgcatct gccgcgcggc cgtgggcggc atgctgcccg acgacgacgt ctgcatctcg
     7681 aagctcgcgc cgccatgcgg gctgtgccag ctccacgtgc ggacctgcat tgggccgacg
     7741 gccgtctgga cgacgcgcgt atcggttttc acgggcacgg cctgcccatt gacattgacg
     7801 aacatcggtt gtgctgccgg agcggcgtcg gcctgcccgg ccagggcgag cacgggcatc
     7861 aacgccagcg tgcccgccgc catatacgaa cggaatcgca tgttcggatc tccatcaaac
     7921 gagactgatc gaatcaacaa tgcacgaatc gtgcggctca cggactgagc cacgaaggac
     7981 tatgcgagcg ccggcttaaa tctggcttaa aaccggggcg ggaccgcgga gcgagggccg
     8041 ggtggggcgc tcgggcgatt cgccgacgcc cgtgaacagc ccgttttgcg cgtttcgaga
     8101 tatgccgcaa tcggcatttc aacctcgccc gcgccatggt attgccgtcg cgccgcgcca
     8161 caacaggcac aatacgcgct tcgcttcgcc gcgcaccgct gttcgagatt cgaacaagcg
     8221 ggcgcgtgtc cccgctgtcg aaatccttcc cggttgtcat gaacgcctcg ctcactcccg
     8281 cgcgcgaatc gaactcgccg cgcttcctgc tgtttctgat ttgcctgttc tcgtccgccg
     8341 gccagctcgc gatcgacatc tacgtgcccg cgctgcccga catggcgcgc tcgttcgcga
     8401 ccacgccgca ggcgatccag tcgagcgtgt ccggctatat ggccgcctat gcgctcggcc
     8461 agctgatctt cggcccggtg gccgatgcgt acggacgcaa gcgcgtgctc gcgttcgggc
     8521 tcgtgatcta cacgatcggc tgcctgctgt cgctcggcgc gccgaacctc gagacgttca
     8581 tactggcgcg ctgcctgcag ggcttcggga tcgcgacgac gaacctgctc gcgaaggcga
     8641 tcatcaccga ttcgttctcg ggccaggcgc tgatgcatgc gttcacctac atgtcgatcg
     8701 cgtgggggct cgcgccgatc atcgcgccgg tgatcggcgc gcacctgcag gaatggttcg
     8761 gctggcgcgc gtgcctcgtg ttcctgctgg tgtattcgct ggtgatgtgg gcgctgtggt
     8821 ggcattaccg ggaaaccttg ccgaaaccgg tccatctcga gccgcgcacg ctgatcgcga
     8881 acgcgggcaa ggtgctcggg agcccggtgt tccagagctg cttcctcgcc caggggctgt
     8941 gctacagcat cctgctggtg ttcaacatcg tcgggccgtt catggtgcag accacgctgc
     9001 acaagccgcc gaccttcttc ggctatctcg cgctcgggat cgggctgatg tatttcctcg
     9061 gtgggctgtc gaaccggatt cacgggcgcg ggctgccgag cgcggagcag cggctgcgca
     9121 tcggcgcgcg cgtgatggcg gtcgcggcgg tcgtgatgct cgtgctggcg ctgaccgtcg
     9181 gcctgcgtgt gtggacgctc gcgacgccgg tgctcgtgat gggcctctgc gcgggtgcga
     9241 tgtatccgac gctgatggcg aagggcaatt cgctgtttcc gcatatcgcg gggctgacga
     9301 gcgcgatcct cgggtgcgcg ctgctgctcg tatcgtccgc gatgatgggg ctggccggct
     9361 tcgtgtcggt gcaggtgctg acgccgctcg cggtgttctt cgtcgcgctg tcgttcatcg
     9421 tcgtatggat ggtgacgaag ctgctgcgct acctgacgca gcagcaagcc gcgagcgtcg
     9481 cttgcggaag cggggaggcg gcgtaagccg aatcaaccgg taccgcgcag gagccttgcg
     9541 tttatagcgg atgcgcggcg ccggcctgcg tcagcagcga aacggcaacc ggatccggca
     9601 tcccgttcga atcgatcgcg ccggccttgg ccagtgcccc aaggtctgcg tcgacgcccg
     9661 gttgcccgac cacgaacggc gtggtcagaa acgccggcgt cgcgagcgtg accgagtagc
     9721 gctgctggag attcgtcacg acggccgact gcgcggcctg cacggtgccc ggatcgccca
     9781 tcacctgctg gaagcgccga cttccctgga aattgccgat cagcccggcc gtgttcgtgc
     9841 cgagctgcgc ggcgagatag acgagcatca gctcggtttc gggcgtcgtg ttgaaggtgc
     9901 cgcccgcgta ggcgagcgaa tgcaggctcg tgccgcccga cgcgacgctg atcacgcacg
     9961 gcagcacggc gttgacggtc acgcggtagt tgccgccgcc gtcggtcagc gtcgtcgcgg
    10021 agccctgcgc gcaactcacc gtcaccgatg cgctcgcgag cgcgccgccg gtggcggccg
    10081 tgccggacag cgcgacggtc tgcgtgttgt tgtcgttgaa gcaggcatcg acgccgaagc
    10141 aggcctcgcc gttgcagccc gccagtgtcg cgagagcggc gacgcaaagt gcgcccagcg
    10201 cggggcgggt gaagtgcggc gggcgaatgt tcatggtcgc gggcgatcgg gggaagaaga
    10261 caaccggtga gcgcagggca tacggccctg ctcacattct aggtgccgaa tcggcgcgtt
    10321 gcttgtccgt ttgacgcagg cgcgcgaatt ttcgaagcgc accggcaatc gatttcccgc
    10381 ccgcccgccg aaggctaac
//
