LOCUS       NZ_CP066711            12151 bp    DNA     circular CON 06-APR-2024
DEFINITION  Staphylococcus pseudintermedius strain DSP030 chromosome, complete
            genome.
ACCESSION   NZ_CP066711
VERSION     NZ_CP066711.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 2612059)
  AUTHORS   Ferrer,L., Perez,D., Fonticoba,R., Vines,J., Fabregas,N.,
            Madronero,S., Meroni,G., Martino,P., Martinez,S., Cusco,A.,
            Migura,L. and Francino,O.
  TITLE     Whole genome sequencing and de novo assembly of Staphylococcus
            pseudintermedius: a novel pangenome approach to unravel pathogenesis
            of canine pyoderma
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2612059)
  AUTHORS   Ferrer,L., Perez,D., Fonticoba,R., Vines,J., Fabregas,N.,
            Madronero,S., Cusco,A., Migura,L. and Francino,O.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2020) Servei Veterinari de Genetica Molecular,
            Universitat Autonoma de Barcelona, Edifici V, Travessera dels
            Turons, Bellaterra, Barcelona 08193, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUL-2020
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 164x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016455265.1-RS_2024_04_06
            Annotation Date                   :: 04/06/2024 05:27:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,396
            Genes (coding)                    :: 2,343
            CDSs (with protein)               :: 2,343
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 31 of 53
            Pseudo Genes (incomplete)         :: 19 of 53
            Pseudo Genes (internal stop)      :: 7 of 53
            Pseudo Genes (multiple problems)  :: 3 of 53
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:24:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2145243
            Orig. end                         :: 2157394
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066711.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12151
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12151
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12151
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7151](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7151)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(149..1930)
                     /gene="recQ"
                     /locus_tag="JGZ56_RS10110"
                     /old_locus_tag="JGZ56_10110"
     CDS             complement(149..1930)
                     /EC_number="3.6.4.12"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recQ"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424488.1"
                     /locus_tag="JGZ56_RS10110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_10110"
                     /product="DNA helicase RecQ"
                     /protein_id="WP_100006801.1"
                     /transl_table=11
                     /translation="MEQTLSHYFGYKSFRPGQKEIIERVLNHHHTLGVLPTGGGKSLCY
                     QVPGLMFKGTTIVISPLISLMKDQVDQLTAMGISAAYLNSSLSTQAQKEIETQLVNGEL
                     KFLYIAPERFEQTYFEMLLRRVDISLVAFDEAHCISKWGHDFRPSYQAVIQRVLTMPQQ
                     FSIVALTATATTEVQQDIMERLMISRQHLVKTSIRRRNLKFQVNGTYQRQKFVLDYIQK
                     HSQKAGIVYCSTRKQVEALYEALEDADIPVSYYHAGMTAKQRDEAQNDFLYDRTRVVVA
                     TNAFGMGIDKSNVRYVIHYNMPGDLESYYQEAGRAGRDGLDSDCILLYSDRDIALHQYF
                     IGASKADDDYKERMGEKLTKMIQYTKTTKCLEATLVHYFEPNEKLEECHQCSNCIDKNK
                     TYNMTKEAKMIISGVARLRQQEGYQTVIQVLRGEVSDYIRHQGYENLSTYGLMKDYTTG
                     ELHHLIDELRFKGFLNEHDEILMCDASVEKLLSEDTQVFTRPFKRKSKETVHINTVEGV
                     DRTLFEKLAEVRREMSERLNMPPTNIFTDYTLEAFAKRKPVTKQEMIQIDGVGSYKLKH
                     YCPEFLEAIQEYKMHRS"
     gene            complement(1940..3817)
                     /locus_tag="JGZ56_RS10115"
                     /old_locus_tag="JGZ56_10115"
     CDS             complement(1940..3817)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166146.1"
                     /locus_tag="JGZ56_RS10115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_10115"
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="WP_015728743.1"
                     /transl_table=11
                     /translation="MEAYKIERLNKSYADKVIFDDLQLSISNQERIGLVGINGTGKSSL
                     LKVIAGMDEDFDAVQSHPKQYRIRYAAQNHNLEPTLTVYEAVYSADSKELQVIKRYEEA
                     TAQYAEQQTDKAFDAMMAAQADMDAYSAWDYSAEIKTILSKLGIHDTTRRMSELSGGQQ
                     KRVVLARTLIEKPDLLLLDEPTNHLDFESIHWLIQFVKSYPKTVVFVTHDRYFLNEVAT
                     RIVELRNGKLDTYPGNYEAYIAMRAEREEIEVRQQTKQRALFKQELAWMRAGVKARTTK
                     QQARQDRFHDLKEQVQSHQVQGKGSLNLAYSRLGKQVFELENVTKRIDGRTLFENVSTL
                     IQKGVQIGIVGPNGVGKTTMLNILAGEDQHFEGVLKIGQTVKVAYFKQTDERLNRDVRV
                     IDFLREESELARQKDGTTVSITQLLERFLFPSVTHGKKVYKLSGGEQKRLYLLKLLVHQ
                     PNVLLLDEPTNDLDTETLTILEDYIQTFGGTVITVSHDRYFLDKVVDCYWYIHDGQIDM
                     ILGQFEDYLAYQKQLEQAATQVASVKQQPTLQPKKKKGLSYNEKREYDMLLVRIEETES
                     RLAEIDAEMIEANADYAKIKALNEEKETLEAQYDTDMTRWSELEEILEQ"
     gene            3929..4744
                     /locus_tag="JGZ56_RS10120"
                     /old_locus_tag="JGZ56_10120"
     CDS             3929..4744
                     /GO_function="GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019168536.1"
                     /locus_tag="JGZ56_RS10120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_10120"
                     /product="ZIP family metal transporter"
                     /protein_id="WP_100006803.1"
                     /transl_table=11
                     /translation="MLDYISNMPPFLQALLAGVITWLLTALGAATVFVFKSVNEKVLNL
                     MQGFAAGIMIAASFWSLLQPAIESSSGSTVPWLPAAIGFLLGGVFIRSLDYVIPHMHRN
                     ATDESQHQEGVETSLNKNTLLLLAITLHNIPEGLAIGVAFGGIATGNEHATLLGALGLA
                     IGIGIQNIPEGAALSLPIHASGQSKWKAFNYGQASALVEPVFATVGAAAVLVVTPILPY
                     ALAFAAGAMIFVVVEELIPDSQAGKNTDLATLSLMVGFTIMMILDVALG"
     gene            complement(5001..7151)
                     /locus_tag="JGZ56_RS10125"
                     /old_locus_tag="JGZ56_10125"
     CDS             complement(5001..7151)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /locus_tag="JGZ56_RS10125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_10125"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_015728741.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2e-38, bitscore:
                     128.4, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 4.5e-271, bitscore:
                     898.5, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFSKRFYRAQVDSQDCGAAALAMILEFYGSHYSLDFLRRKLRTT
                     VNGTTAYGLVQVADKLGFETVPIKADISLFEIDDVQYPFIVHVKKNNNHFHYYVVIRCD
                     KKYIYIADPDPVVNLTKMTYREFKEEWTGVTILLKPTSLYKPIKQDKVRLTSFIPLLLK
                     EKRLIIKIIIISILVNMINILGSYFMKSIIDFYIPQKLKTWVSMISMALIASYIIQQIL
                     SYLQKYFLILLVKTYSINILSPYIKHLFRLPITFFETRRAGDILSRLNDANSITDALAS
                     TMLSLFLDVTIIVGISIFLYYQSTILFVLILIIIPIYAVIVFGFMNSFERKRIKSMEAN
                     ATLSSYLMENITGMETIKALTCEKKCYKQIKYNFSNYYEKTFLLNKLNIKQNVLKKVVQ
                     LILNVSILWVGANLVIDNQISIGTLLVFNSLVIYFMNPIENIMNLQLKLQIAGVANNRL
                     NEIFTVKSEFEKNKLDIKLNQNSSKLIFSNISYGYGYEKEILTDINITIEHNSKVAFIG
                     ESGSGKTTLAKMMVSFFEPSKGKITLGGLDITNINKSVLRQQIIYIPQQTYIFNGTILE
                     NLLLGVVKEVTQKDIDSAIKMAELKSFIDSLPLGLDSVLSSDFSALSGGQRQKIALARA
                     LLTNSSILIMDEATSNIDILTERKIINNLMALNKTIIFISHNLNITQKVDKIFVLAKGK
                     IIEEGSHEFLLKAKGYYSNLIN"
     gene            complement(7276..7458)
                     /locus_tag="JGZ56_RS10130"
                     /old_locus_tag="JGZ56_10130"
     CDS             complement(7276..7458)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JGZ56_RS10130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JGZ56_10130"
                     /product="hypothetical protein"
                     /protein_id="WP_037543780.1"
                     /transl_table=11
                     /translation="MKNIDKFKVISNEVLKDLSGGMSRCKWAQIACINDSMLVRFGTSD
                     PESACDYMKKICKDE"
     gene            complement(7530..7838)
                     /locus_tag="JGZ56_RS10135"
                     /old_locus_tag="JGZ56_10135"
     CDS             complement(7530..7838)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024424.4"
                     /locus_tag="JGZ56_RS10135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_10135"
                     /product="DUF3884 family protein"
                     /protein_id="WP_049770161.1"
                     /transl_table=11
                     /translation="MIKISLKKILCQLSQKKLYEKTFQSIYIVDFSLLDRVPLFKDEFK
                     VIGTWYSYSGKRWICHTELSTEQFKKMITKNIDHKDLKKVKFYLDYLPFSITNEIPF"
     gene            complement(7910..9043)
                     /locus_tag="JGZ56_RS10140"
                     /old_locus_tag="JGZ56_10140"
     CDS             complement(7910..9043)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /locus_tag="JGZ56_RS10140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_10140"
                     /product="glycosyltransferase"
                     /protein_id="WP_187536068.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.7e-18,
                     bitscore: 64.2, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEKINKLEIISEYTTISVVYIVKNERDYIIKSLLSILDLADEII
                     IVDTGSEDNTLDLIKKMCDKKIKIFTFNWCDDFSKARNFANAKATCDWIMILDADEIVR
                     KNDNLKFYLTYLRIFDDFENTAFNFKVIRDEEVYKTSKLIRNTNTLTYKGRVHETFFSL
                     NNSVSYANLNVEVLGIRRGSQKKTNYYNKLLLKTIIDFPKEGRWYYLYLRDNIENMNYN
                     QMLKFCKKIIFKRDAISGIEINNNYYSVRIIFLLMFKLLEESNHEGFIYYMELLEKDFN
                     HTQDFIFLKYSYYIKKLNIDATNLLKSFLENYDLLDEGDVIFSKECTHSLLGAYLFWGG
                     YLKESKKVFEELEVNGKDTVCFYNKKFIYDCLSTLSK"
     misc_feature    10186..10199
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.13"
                     /tool="antismash"
ORIGIN
        1 attcattgta aaaataacac aatttgcatc taaaaaattt aaatcatcta ctatcgacac
       61 gtctcacttt tccattcttt accactcaat aagaaatcgt ttatttgtaa aaaaggagcc
      121 aggaattccc ccagctccat ctgatgtttt acgaacgatg cattttatat tcttgaatcg
      181 cttctaaaaa ttcaggacaa tagtgcttca gcttataact accaacacca tcaatctgaa
      241 tcatttcttg ctttgtgaca ggctttcttt tcgcaaaagc ttctaacgta tagtctgtaa
      301 aaatattcgt cggtggcatg tttaatcgtt cgctcatttc acgtctgact tcggcaagct
      361 tttcaaataa cgtgcggtcg accccttcta cagtgttaat atgcactgtt tctttcgact
      421 tacgcttgaa cggccttgta aacacttgtg tatcctcact caataacttt tctactgagg
      481 catcacacat gagtatttcg tcatgttcgt tgagaaagcc tttaaagcgg agttcatcga
      541 ttaaatgatg taactcgcca gtcgtatagt ctttcatcaa accatacgtc gaaagatttt
      601 catagccttg atgacgaatg tagtccgaaa cctcgccacg caaaacttga atgaccgttt
      661 gatatccttc ttgttgacgt aaacgtgcga caccgctaat gatcattttc gcttctttcg
      721 tcatattgta cgttttattt ttatcaatac aattactaca ttggtgacat tcttcaagtt
      781 tttcattcgg ctcaaaataa tggactaatg ttgcttctag acatttcgtc gttttcgtat
      841 attgaatcat ttttgtcaat ttttcgccca tacgttcctt gtaatcatcg tccgctttcg
      901 aggcaccgat aaaatactga tgtaaagcaa tatctcgatc actgtacaat aaaatacaat
      961 cactatctaa tccgtctcga cctgcacgtc ctgcttcttg ataatacgat tccaaatcac
     1021 ccggcatatt gtaatgaatg acataacgga cattcgattt atcaattccc ataccgaatg
     1081 cgttggtcgc aacgactaca cgagtacggt catataaaaa gtcattctgg gcttcatctc
     1141 tttgtttcgc tgtcattcct gcatgatagt aggatacagg gatatctgcg tcttctaaag
     1201 cttcatacaa ggcttctact tgtttacgcg ttgaacaata aacaatcccc gctttctgac
     1261 tatgtttttg aatataatcc aatacaaact tttgacgttg atacgtccca ttcacctgga
     1321 acttcaaatt acgtcgacga atactagttt tgactaagtg ttgtcgcgaa atcattaacc
     1381 gttccataat atcttgttgc acttcagtcg tcgctgttgc agtcaatgcg acaatcgaaa
     1441 actgttgcgg catggtcaat acgcgttgta tgactgcttg ataactcggc cgaaaatcat
     1501 gtccccattt tgaaatacag tgtgcttcgt caaatgcgac gagtgaaata tcgacacgtc
     1561 gcaacaacat ttcaaaataa gtttgctcaa aacgttccgg tgcgatgtat aaaaatttca
     1621 actcaccatt gaccaattgc gtttctatct ctttttgtgc ttgcgtactt aaactactat
     1681 tcaaataagc cgcagatatt cccattgcgg tcaattggtc cacttggtct ttcattaacg
     1741 aaattaaagg actaatgaca atggtcgttc ctttaaacat caatcccggt acttgataac
     1801 ataacgattt acccccacct gtgggtaaca cccctaacgt atgatgatga ttcaatacgc
     1861 gttcaataat ttctttttga ccaggacgaa aagatttgta tccaaaataa tgcgacaatg
     1921 tttgctccat gctatccctc tattgttcta atatttcttc tagttcactc caccgcgtca
     1981 tgtcggtatc atattgagct tcgagcgttt ctttttcttc atttaatgct ttgattttgg
     2041 cataatctgc gttcgcttcg atcatttcgg cgtcaatttc agccagacgc gactctgttt
     2101 cctcgatgcg cactaataac atgtcatatt ctcgtttttc gttgtatgat aatccttttt
     2161 tcttttttgg ttgtaatgtc ggttgttgtt tcacggacgc tacttgagtt gctgcttgtt
     2221 ccaattgttt ttggtatgct aaataatctt caaactgccc taaaatcatg tcaatttgac
     2281 cgtcgtgaat ataccaataa caatccacga ctttatctaa aaagtaacgg tcatggctca
     2341 cagtaatgac ggtaccacca aatgtttgaa tgtagtcttc taaaatcgtt aacgtttctg
     2401 tatccaaatc atttgttggt tcgtctaaca acaacacgtt cggctgatgc actaacaact
     2461 tcaataaata caaacgcttt tgttcgccac ccgagagttt atacactttc ttaccatgcg
     2521 tcacactggg gaataaaaag cgttccaaca attgcgtaat cgaaacggtc gtcccatctt
     2581 tttgacgcgc caactcactt tcttcacgca agaaatcaat gacacggaca tcacgattca
     2641 atcgttcatc tgtttgttta aaatatgcca cttttaccgt ttgacctatc tttaatacac
     2701 cttcaaaatg ctgatcttca cctgctaaaa tgttcagcat cgtcgttttg ccgacaccgt
     2761 ttgggcccac aataccgatt tgaacacctt tttgaatcaa tgtcgacacg ttttcaaata
     2821 atgtacggcc atcaatacgt ttcgtcacat tttcaagttc aaagacttgt ttgcctaaac
     2881 gcgaataagc cagattcaat gaccctttcc cttgcacttg atgcgattgt acttgttctt
     2941 tcaagtcatg aaaacgatct tgccttgctt gttgcttcgt agttcgcgct ttgacaccgg
     3001 cacgcatcca cgctaactct tgtttaaaaa gtgcacgttg tttcgtttgt tgacgcactt
     3061 cgatttcttc ccgctctgct cgcattgcaa tatacgcttc atagttacct ggatatgtat
     3121 ctagcttgcc atttcgcaat tctacaatac gcgtcgccac ttcatttaaa aagtaacggt
     3181 catgcgtgac aaaaacgact gttttcggat aagactttac aaattgaatt aaccaatgaa
     3241 tcgattcgaa atctaaatgg ttcgtcggtt cgtctaataa taataaatct ggtttttcaa
     3301 tcaacgtacg cgctaacact acgcgctttt gttggccacc tgagagttca ctcattcggc
     3361 gtgtcgtatc atggatgcca agtttcgata aaatcgtttt aatttcagca ctataatccc
     3421 aagcactgta agcatccata tcagcttgtg ctgccatcat tgcatcaaac gctttgtcag
     3481 tctgttgctc tgcgtattgt gcagtagctt cttcataacg tttaatgact tgcaactctt
     3541 tggaatctgc actataaacc gcttcataaa ccgttaacgt tggctccaaa ttatgatttt
     3601 gtgccgcata acgaatacga tattgtttcg gatgcgattg aacggcatca aaatcttcat
     3661 ccatgcctgc gatgactttg agcaatgaac ttttccccgt tccattaata ccaactaatc
     3721 cgattcgctc ttgattggaa atggataact gtaaatcatc aaaaatgact ttatccgcat
     3781 aagatttgtt gaggcgttca attttatacg cttccacaac acatacaccc tttttcttat
     3841 agatttactc gcatttatct gttaacctta aatcagttct atatattata cacttttttt
     3901 ggaacaaatg atacaaggag ctatttctat gttggattat atttcaaaca tgccaccctt
     3961 tttacaagca ttacttgcag gtgtcatcac gtggttatta accgcattag gggctgcaac
     4021 cgtatttgta tttaaaagtg tcaacgaaaa agtgttgaat ttgatgcaag gctttgctgc
     4081 tggtattatg atcgccgcaa gtttttggtc attactccaa ccggccattg aaagcagttc
     4141 gggcagtact gtaccgtggc tccctgctgc aattggcttt ttactcggcg gggtattcat
     4201 ccgttcttta gactatgtta ttccacacat gcatcgaaac gcaactgatg aatcacaaca
     4261 tcaagaaggt gttgagacga gtctaaataa aaacacactt ttattacttg cgattacatt
     4321 gcataacatt cctgaagggc tcgcaatcgg ggttgccttt ggtggtattg cgacaggaaa
     4381 tgaacacgca actttgttag gggcactcgg acttgcaatt ggtattggga ttcaaaacat
     4441 tcctgaaggg gctgcattat cattaccgat acatgcttca ggacagtcca aatggaaagc
     4501 cttcaactat ggacaagctt cagcacttgt agaacctgtt tttgctacag tgggcgcagc
     4561 tgctgtgttg gtcgttactc cgatactacc atacgcactc gcttttgctg caggggcgat
     4621 gatttttgtc gtcgttgaag aattaattcc tgattcacaa gccggtaaaa acacagatct
     4681 tgcgacacta agcttgatgg ttggctttac catcatgatg atattagatg ttgctttagg
     4741 gtaaattcaa aaaaccgttc aatgtgatga aacactgagc ggttttttga tgtgtacata
     4801 tatcgataca ttaaaaatac tctttaacaa ctcacaacaa agatttcaca ctgttgacta
     4861 tataaaatat caaccaatat accaaaatat aataattaac ttaagttata attgtaaaat
     4921 tcagaaaata aaaggtgtga aaaaacagta ccttcatttc gttcttcaca ttggttgtgt
     4981 taatacaaaa aataccatta ttaattaatt aagttagaat agtaaccttt tgcttttaat
     5041 aaaaattcat gtgagccctc ttctataatt tttccttttg ctaaaacaaa tattttatct
     5101 actttttgag ttatatttag attatgggag ataaatataa tagttttatt taacgccatc
     5161 aaattattaa taatttttct ctccgtgagt atatcaatat tactcgttgc ttcatccatt
     5221 atcaatatag aagaatttgt taatagtgct cttgctaaag ctatcttctg ccgctgacct
     5281 cctgacaaag cagaaaaatc agaacttaat acactatcaa gtccaagtgg taagctatca
     5341 ataaaggatt ttagttcagc cattttaata gcactatcta tgtccttttg cgtaacctct
     5401 ttaactaccc ctaataaaag attttcaagt atagttccat taaaaatata agtttgttgt
     5461 ggaatgtata taatctgctg tcgtaaaaca gatttattga tatttgttat gtccaatcca
     5521 cctagtgtta ttttaccctt tgaaggttca aaaaaactaa ccatcatttt cgccaaagtt
     5581 gttttacctg atccagactc tccaataaag gctactttac tattatgttc tattgttata
     5641 ttaatatctg taaggatttc tttttcgtac ccatatccat atgaaatatt tgaaaatata
     5701 agtttacttg agttttggtt caatttgata tccaatttat tcttttcaaa ttctgattta
     5761 actgtgaata tttcattcaa tcgattatta gccacacctg ctatttgtaa tttaagctgt
     5821 aaattcataa tattttctat tggattcata aagtaaatca ctaatgaatt aaaaacaagc
     5881 aaagttccaa ttgagatttg attatctata actaggttag cgcctaccca taaaatcgat
     5941 acatttaata ttaattgaac aacctttttc aaaacatttt gctttatatt tagtttgttt
     6001 aacaagaatg ttttttcgta ataattcgaa aaattatatt ttatctgctt atagcatttc
     6061 ttttcacatg tcagtgcttt tatcgtctcc atacctgtta tattttccat caaataagaa
     6121 gacaaagtag catttgcctc catagatttt attcttttcc gttcaaaaga gttcataaaa
     6181 ccaaaaacaa ttacggcata aataggaatg ataattaata ttaatacaaa taaaatagta
     6241 ctttgataat acaggaagat agatattcca actattattg taacatctaa aaacaatgat
     6301 agcatggtac tagctaaagc gtcagtaatt gaatttgcat cattaagtct tgatagtatg
     6361 tctcctgctc ttctcgtttc aaaaaaagta ataggtaatc taaacaaatg tttaatgtat
     6421 ggtgaaagta tatttataga atatgtcttt actaataaaa tcaaaaaata tttctgcaaa
     6481 taactaagaa tttgttgtat aatgtaagaa gcaattaaag ccatggaaat catactaacc
     6541 catgttttta acttttgcgg aatataaaaa tcaataattg acttcataaa atatgaacct
     6601 aaaatattta tcatattgac taaaatggaa ataattatta tttttataat caaccttttt
     6661 tcttttaata ataaaggtat aaaagaagtt aaacgaactt tatcttgctt tataggttta
     6721 tataaagatg taggttttaa taagattgta actccagtcc actcttcttt aaattcccta
     6781 taagtcatct ttgttaaatt aacaacaggg tcagggtcag ctatatagat atacttttta
     6841 tcacatctaa ttacaacata atagtgaaaa tgattgttat tttttttaac atgaacgata
     6901 aaaggatact gtacatcatc aatttcaaac aaagatatat ctgctttaat aggaactgtc
     6961 tcaaaaccta acttatctgc tacctgaacc aaaccatatg ctgtagtgcc atttactgtt
     7021 gttcttaact ttcttctcag aaaatccaaa gaatagtgag aaccgtaaaa ttctaatatc
     7081 atagcgagcg ctgccgcgcc acaatcttga gaatctactt gtgcacgata aaatcgtttt
     7141 gaaaatttca ttcgacactt ctactccttc ccaccacttt ttagaacaac atttaaatat
     7201 tcaaaaagcg ggcggtaatt cccacccact taatatcatg tcaaaattca atcctattat
     7261 taaaagcttt aatggttatt catctttaca tatttttttc atataatcac atgcactctc
     7321 tggatcagat gtaccgaatc taaccaacat actgtcgttt atacatgcaa tctgagccca
     7381 tttacaacgg gacatcccac cacttaagtc tttcagtacc tcatttgata tcactttaaa
     7441 tttatcaata tttttcatat ctattcctcc taaacaaaac aatttaataa taatattaaa
     7501 aattaattca ttaaatatat aatttatttt caaaacggaa tctcgttagt tatagaaaat
     7561 gggagataat ccaaataaaa tttgactttt tttaaatctt tatggtctat attttttgtt
     7621 atcattttct tgaattgctc ggttgataat tccgtatggc atatccaacg cttaccacta
     7681 tagctatacc aagttcctat tactttaaat tcatctttaa atagaggaac cctatctaaa
     7741 agactaaaat caactatata aatactttga aatgtttttt cataaagttt tttttgagat
     7801 aattgacata aaattttctt taaagatatt ttaatcatag atttctcact ccttcacact
     7861 acctgttacc ttaatgcggt agattcctga gctataataa tatcaatatt tatttgctta
     7921 aagtcgatag acaatcataa ataaattttt tattataaaa acaaactgta tctttaccat
     7981 ttacttctaa ttcttcaaat acttttttac tttcctttaa atatcctccc caaaataaat
     8041 aggctcctaa taacgaatga gtgcattctt tcgaaaaaat tacatctccc tcatctaaca
     8101 aatcataatt ctctaagaaa cttttcaata aatttgtagc atctatatta agtttcttta
     8161 tatagtatga atatttcaaa aaaataaagt cttgagtatg attaaaatct ttctctaaca
     8221 actccatata gtaaataaaa ccttcatgat tggattcttc taataactta aacatcaaaa
     8281 ggaaaataat tctaacagag taataattgt tgtttatttc gattccagat atcgcatctc
     8341 ttttaaatat aattttttta caaaatttta acatttgatt ataattcata ttttctatat
     8401 tatctctcaa atataaataa taccaacgac cttcttttgg gaaatctata attgttttaa
     8461 gaagcaattt attataataa tttgttttct tttgacttcc tcttctaatt cctaaaactt
     8521 ctacatttaa attagcataa gatacagaat tatttaaaga aaagaatgtt tcgtgtactc
     8581 tacctttgta cgtcaaagta ttagtatttc ttatcaactt acttgtttta tatacttctt
     8641 catctctaat tactttaaaa ttaaaggccg tattttcaaa gtcgtcgaaa attcttaaat
     8701 acgttaagta aaactttaaa ttatcatttt ttctaacaat ttcgtcagca tctaaaatca
     8761 taatccagtc acaagttgct ttagcatttg caaagtttct tgcttttgaa aaatcatcac
     8821 accaattaaa tgtaaatatt tttatttttt tatcacacat ttttttaatt aaatctaaag
     8881 tgttgtcttc agaaccagtg tcaacaataa tgatttcatc ggctaaatct aaaatactca
     8941 aaaggctttt tataatataa tctctttcgt ttttaacaat atacactaca gaaattgtag
     9001 tatattcact tataatctct aatttattta ttttttcaat caattctgta tcgtttttaa
     9061 ctcttttaga ctcccaatct tttgattcta taaatttatt ttgttgtatg tttcacaaga
     9121 aatgttaaga aatgccttat ttttttctaa ccaatcttca tcaaatatag ggtatatgtt
     9181 tttcaaagtt aatcacaccc cctcaaaaaa aaataataaa acaaaatatt tttaaaagtt
     9241 ttgcaagact gggaggcgaa ttattatgat aaatacgtaa ttctactatc acatttacaa
     9301 attgctatgc tttaattata aataattgta ttactttgta aatatagtgt caatatttat
     9361 ttatattcgg aagtataatt tctcacatat actttaatta cagaagtaca tgcagtgtag
     9421 atgacggtta taaaatgtag gaaaatggcg aaatgaaaat gctgcatttc atatataaac
     9481 gccaagataa atatggacaa gtttattctt tttatgaaag gaaatgtttt aatctaaaat
     9541 actttccaac gatagaggcg attgagacgg gttctttgtc aacgtcggtt ggtgagacaa
     9601 taacggcact gagacattat ggtttcctaa tctctgcttc tatttataga ctgatatcga
     9661 ttaataatga ttgatcattc gaaaataagg ggtgaatgtg tataaaaaat gatgccattg
     9721 ttttctggat ttcataatct tgcactcagt ttcgctgtat tgatatagga ttacccaaat
     9781 ggctgagacg cctgaggggg aacgtgcata ctgttgacag tcgcctcttt actttaatgg
     9841 tgggaggcgt ttatcgcagt agcgggctga cttctcagtg ttttcacttt cgagaagtct
     9901 aatcagccat gcaaagcagt acaacgaaat cttgttgcat atgagatttc tgtactgctc
     9961 ctaaaatcca atttggcttc aatcaagccg aattgagttg aagggccggc tctaggaaag
    10021 cgaaaccatg atgacaatta aaaccacaaa tcgtaatgcg gacaagttta ataaaaatat
    10081 tacaacaata gcatcaaaaa tttttaagac ccattccgtt ttttatattc acttacaagt
    10141 atcttagaaa tgattaagat tccagaaatt tctataacta taagacacac gtttgtttta
    10201 ttattaatcc tttcaatcgg gccatttgtt aaatgaggat gatgcatagt atttgaggtt
    10261 cagggcaaat atttgatgaa tgtttgcaac gattaggtaa aatgtcgatg atatcatggg
    10321 ttacactatc acagtaaata aagctcattg gtccttcgat atctttcgtt gatttgaatt
    10381 cgtcaaaagc taagtgctca ggtaaagcat catgtgcgta tgtctttaca aattactcac
    10441 ctcttttaaa tggcaatgaa cagttgaagg tgatacacat agacttttag cgacatcttt
    10501 ttcagatgtg aatttagtta gcttccgcgt catcaagcgt ttgaccttat ttgagacttc
    10561 ggggttgaat atagggtgtt ttagttataa aggtttgttg acaagcttta aagtaaaaac
    10621 gatgcttctt caaatttaaa taaacaggtc tttcaaaatt aaaaaacggg cagtaaatct
    10681 ctcaccaacc gttaaaaata cacaaccaaa tatatacaac ctaaaaaaca gggaaatgca
    10741 ctgatttgta cacttcccta tcataacatt ttatttttga tttgcctttg gacctgtcgg
    10801 atatttataa tctgctgggt tcaatttgtt gaaatcagga tttttataga atcggaataa
    10861 gtctccattc aagactttat cactcatttc taagtcatgt tgtacctgct ttttgtcctt
    10921 atcaagatct gctggcggtg tttctaacgg ttgattgtct ctgttactgt aggctttacc
    10981 gttgacatat ttataatttc tcgtcacgaa atcaccatta cggaatggta caatttcttt
    11041 atggtccttg gataacatat ctgtaccaaa catgagataa tttttcgtat caatacctaa
    11101 caagtgaagt aatgttggca tcacatcaac ttgacctgca tacgtgtcgt ccactttagg
    11161 ctcaacccct ggtgctttta accagaaacc cgttgcattc aagtcattaa acttcgctgg
    11221 tgtaatctct tcacctaata actgtgacat cgctttgtta tgattttctg agataccata
    11281 atggtcaccg taaatcataa tgactgaatc atcgtacaga ccacgctctt tcaagtcatt
    11341 aatgaactgt tctaacgact catctaagta acgcgctgtt tgaatgtaac catcgaccgt
    11401 tgagttacct gtgtcacctt ttgcaatcgt tgcgtcttca tcacttaatg taaaaggata
    11461 atggttcgtc aacgtaatta aatgtgaata gaacggttgt ttttctttag ataagtaatc
    11521 cgccgcctct ttgaaaaact ctttatcttt taatccaagg ttttctaagt tttctggaga
    11581 catgtcaaaa tatgttgcat cataaaactt atcgatacca aaatgtctgt atacttggtc
    11641 acggttccaa aatgttttat agtcaccgtg catgacattt gttgtatagc cttgttgttg
    11701 atgcataata gctggtaaag attgaaaagt attgtcacct tttaatgaaa aggccgaacc
    11761 ttgaggtaaa ccatacatac tgttgtccat tgtgaactca gcgtctgacg ttttaccttg
    11821 tccagtttgg tgatagaaat gtgggtaata tctaaagtcc tcattacctg acgacaattt
    11881 atttaaaaac ggcgtcactt cctcaccatg aattttttta ttaattagga atgtttggaa
    11941 gctttctaaa tggattttaa tgacattttt acctttagct tttccatagt attccatatt
    12001 cggtgtagct tgtttttgtt ttgtataatt caatacttct gttaaatcat cttcagaagc
    12061 gagtgccttt tgttgattgt tctgtactgt tttgacacca tcatatactg taaagttgta
    12121 aggtcctaag tatttcacaa gatatttatg g
//
