LOCUS       NZ_CP066711            11044 bp    DNA     circular CON 06-APR-2024
DEFINITION  Staphylococcus pseudintermedius strain DSP030 chromosome, complete
            genome.
ACCESSION   NZ_CP066711
VERSION     NZ_CP066711.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 2612059)
  AUTHORS   Ferrer,L., Perez,D., Fonticoba,R., Vines,J., Fabregas,N.,
            Madronero,S., Meroni,G., Martino,P., Martinez,S., Cusco,A.,
            Migura,L. and Francino,O.
  TITLE     Whole genome sequencing and de novo assembly of Staphylococcus
            pseudintermedius: a novel pangenome approach to unravel pathogenesis
            of canine pyoderma
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2612059)
  AUTHORS   Ferrer,L., Perez,D., Fonticoba,R., Vines,J., Fabregas,N.,
            Madronero,S., Cusco,A., Migura,L. and Francino,O.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2020) Servei Veterinari de Genetica Molecular,
            Universitat Autonoma de Barcelona, Edifici V, Travessera dels
            Turons, Bellaterra, Barcelona 08193, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUL-2020
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 164x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016455265.1-RS_2024_04_06
            Annotation Date                   :: 04/06/2024 05:27:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,478
            CDSs (total)                      :: 2,396
            Genes (coding)                    :: 2,343
            CDSs (with protein)               :: 2,343
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 31 of 53
            Pseudo Genes (incomplete)         :: 19 of 53
            Pseudo Genes (internal stop)      :: 7 of 53
            Pseudo Genes (multiple problems)  :: 3 of 53
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:24:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 913139
            Orig. end                         :: 924183
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066711.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11044
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6044](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6044
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(320..334)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 23.65"
                     /tool="antismash"
     misc_feature    321..332
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.25"
                     /tool="antismash"
     misc_feature    816..825
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            1416..2348
                     /locus_tag="JGZ56_RS04045"
                     /old_locus_tag="JGZ56_04045"
     CDS             1416..2348
                     /EC_number="2.7.1.107"
                     /GO_function="GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166450.1"
                     /locus_tag="JGZ56_RS04045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_04045"
                     /product="diacylglycerol kinase"
                     /protein_id="WP_014613461.1"
                     /transl_table=11
                     /translation="MRKRARIIYNPTSGKEVFKRALPDVLIKMEQAGYETSAYATQKVG
                     DATEEAARAIEAQYDLLIVAGGDGTLNEVVNGIAEKPNRPKLGLIPMGTVNDFGRALHL
                     PTDIFEAVDVILDGKTVQVDIGKMNSRYFINLAGGGKITEVSYEAPSKLKSIVGPFAYY
                     IKGFEMLPQMHAVDVRIEFDSQVFEGEIMLFLLGLTNSMAGFEKLVPDAKLDDGMFTLL
                     IVEKANIAELGHIMTLASRGEHIKHPKVHYHKAQSVNISSFSDMPLNVDGEYGGQLPAN
                     FLNLVRHIEVFSPAQEDNALLIDEPTQSE"
     gene            2520..3884
                     /gene="rlmD"
                     /locus_tag="JGZ56_RS04050"
                     /old_locus_tag="JGZ56_04050"
     CDS             2520..3884
                     /EC_number="2.1.1.190"
                     /GO_function="GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rlmD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613462.1"
                     /locus_tag="JGZ56_RS04050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_04050"
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="WP_096548478.1"
                     /transl_table=11
                     /translation="MVLVKKNEVYEGKVVDLTHEGHGVIKQDRYPIFIPQTLIDEEVQY
                     KVIKVKKNFAIGKLMNVKTASPDRVTPPCDYYQQCGGCQLQHLSYRAQLEMKRKQVINL
                     FHHKGKMTEVPIHDTIGMDDPWRYRNKSQIPVGTNRNGQIQMGFYRQRSHDIIDMDHCL
                     IQNDDQNDMMNVIKSLLEKYHIPAYNEHIHQGLVRHVVIRKGYYTNEVMVIFVLNGKKL
                     PHHNDIVTALTSQFQQIVSIKLNFQTNKTNVIMGHTSKTIFGKDIIEDTLDDLTFEIGD
                     LSFYQINVTQTQKLYRQALQYANLTGNEVVLDAYCGIGTISLFMAPHAQHVYGVEVVPE
                     AIEDAKRNARYNQLNNTTFVAGAAEDVILKWQQDGIQPDVVTVDPPRKGCDATFIETLN
                     ALAPKRIVYVSCNPSTQLRDIEMLSAQYELKEMTPVDMFPHTTHVETVALLVRKY"
     misc_feature    4034..4052
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.21"
                     /tool="antismash"
     misc_feature    4114..4128
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 26.38"
                     /tool="antismash"
     misc_feature    complement(4139..4154)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 23.45"
                     /tool="antismash"
     gene            4199..4948
                     /locus_tag="JGZ56_RS04055"
                     /old_locus_tag="JGZ56_04055"
     CDS             4199..4948
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JGZ56_RS04055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JGZ56_04055"
                     /product="hypothetical protein"
                     /protein_id="WP_037542633.1"
                     /transl_table=11
                     /translation="MGGINCGLSNNRVVKNDMYRLEYALYTKYVFKCFENFCNANTWVE
                     EFTLLEIDDNDNALVAYKKALFAIQEAITNLQSAKNALINSYNEIAPLFKFSQLKNGSI
                     NQYKKYDHSFAKGVFEKAGVVTNETKVWESVIHSLQNGGEVDYLNDQQNKLIVFENTLQ
                     NLFEEYQKLEKYIRQGVSHVAIRDIEVDITPLTAMALTKISELMSSLTYLCLVEYSAHT
                     SITGKTIDVLDLLPKTNKNSIQHLKAN"
     gene            5001..6044
                     /locus_tag="JGZ56_RS04060"
                     /old_locus_tag="JGZ56_04060"
     CDS             5001..6044
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="JGZ56_RS04060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_04060"
                     /product="YcaO-like family protein"
                     /protein_id="WP_014613464.1"
                     /sec_met_domain="YcaO (E-value: 2.5e-22, bitscore: 77.2,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLENVSEHGVSGFYTYHFTEGNSFRPCATRIIEKEAIKATKDEFM
                     ELMLISSKSFPFVFFGGKSLGNFKLPIHLHPFMWQKRTIQDVNWYNEKNVEYIPFYNYK
                     DGSLKYIPKELSVYYEKAKGIGLSFSPQEGYSYDRDIVLAKVKAVMELIEKDIVTLWWH
                     RETSSRIIVIDDDTDENIKIIRNQLLNQGIDITLLDIENDLGVYVVVCILKQDDFPSIT
                     YGSAAHFEIKGAVKHAIFEAISCIVGLRYEFIHFKKIRTSFTYPDFISDSPLTNLSDYK
                     EILNLKTAINKYDIYYSYISADEGYLVKAYCYELQPTLYSATVPLTKRFFRVNKKEVRI
                     KEDFPFI"
     gene            6055..7245
                     /locus_tag="JGZ56_RS04065"
                     /old_locus_tag="JGZ56_04065"
     CDS             6055..7245
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="JGZ56_RS04065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_04065"
                     /product="MFS transporter"
                     /protein_id="WP_014613465.1"
                     /transl_table=11
                     /translation="MKTKRIIASDFLTTMMSSALTIYIYWFAYQYFSSQLIISIIGFGQ
                     LSGVFLSSLGGGISDSMNKLFFIKTLNFFKVGLLFMVLILENVVNMEILLPLFMFASTV
                     IGGLQSPTLESLVPFLASSDEALFRINAVVSSSTQVASIMAIVLSAVYISFLSFVTIVF
                     VTLFITAIATILLVGLTVETPLKSTSVVNNMKHGASYIFKTPYIRNLIPVALVMNFSFW
                     SIFLLLPKIANDSFSFLKTSYSGMELSFALGAGLGGILFAKFFYDVKDKYRLFKRSLFS
                     QSYVLLLLGIVLLIPNSVLAYTGMLLCWFLYALINTIFSILYFGKIQLNVPREMIGSVF
                     GAILTLFSLVNPIAAIMSGVLVSHFKIPLLIILLSSLMLLAAWSIRFLADVETVFD"
     gene            7259..8626
                     /locus_tag="JGZ56_RS04070"
                     /old_locus_tag="JGZ56_04070"
     CDS             7259..8626
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="JGZ56_RS04070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_04070"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_172426195.1"
                     /transl_table=11
                     /translation="MSILTLKNIRCEMDGKLLFEADGLTIEEGDVIGLIGKNGAGKTCL
                     LKILAGELSDYAGYISGNRLTYFSELCITEDMTQSGGELSISSFLNALRQSVPLYLLDE
                     PTTYLDQHHLKKVVRTIRRSPSSFCIASHDRAFLDAVATKIWLLEQGKITEYNGSFSDY
                     VLARSMQLSQYEQELKQYQKEKKKIKQSLRMMKEKQEQKKGKPKKMSTSEYRIAGIKTK
                     YGVKQKKLQKRITRQQKKLEQLKQPYQVEDKYDISFLSQLHCLPKRKIIIPSHEAKIGQ
                     KSLWHTPTIHLNSGDKLGIIGRNGAGKTTYLNYLVSVLPSTYKICYFRQNHFQYLDPKM
                     TVYEAIVEATNGYLSEHHIRTLLALLDFKRDKVFRLIGDLSRGEHVKVSLLSLLVSESD
                     ILILDEMTNFLDIKAIEAVEKVLATYSGILIFVSHDQYFVQRLATSILDIDTYKIM"
     misc_feature    8818..8832
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 24.36"
                     /tool="antismash"
     misc_feature    complement(9006..9020)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.13"
                     /tool="antismash"
     gene            <9159..9389
                     /locus_tag="JGZ56_RS04075"
                     /old_locus_tag="JGZ56_04075"
                     /pseudo=""
     CDS             <9159..9389
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613462.1"
                     /locus_tag="JGZ56_RS04075"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_04075"
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /pseudo=""
                     /transl_table=11
                     /translation="DVVTVGPPRKGCDATFIETLNALAPKRIVYVSCNPSTQLRDIEML
                     SAQYELKEMTPVDMFPHTTHVETVALLVRKY"
     gene            9641..10816
                     /locus_tag="JGZ56_RS04080"
                     /old_locus_tag="JGZ56_04080"
     CDS             9641..10816
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF040899.1"
                     /locus_tag="JGZ56_RS04080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ56_04080"
                     /product="radical SAM/SPASM ryptide class RiPP maturase"
                     /protein_id="WP_242058976.1"
                     /sec_met_domain="Fer4_12 (E-value: 8.5e-13, bitscore: 45.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 7.2e-08, bitscore: 29.3,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.2e-07, bitscore:
                     29.1, seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFDYRLREWLDTPSSNKNQFSSDTLYNELLLSESKKKIQISLAI
                     TYSCNLRCSYCFQQNYDHLVRKPITIEALEKILDKISLILYQNPEVDVSIGLFGGEPLL
                     PQNEKIIDRVFQYCVENKLKIDITTNGSFLPYFAKKIVIHRSIISVIAITINTLPDKYN
                     ETIKITKVANNVEELLAVTDLLLHYHIVVDVGTNFDRNNLSELLPIYHYFFDKGYFSKW
                     NFHWNIGRVDDRLFDTGYDEYIVSETDILLQLMKIPKPVPSNLHAGFIQTCKNLTDKLN
                     LSFNESQIKGTYHYCWNVSPYDRVFYIDNELNLFRCTVTVGRPQYILGNLKDIDLMNYQ
                     HETKSFLDYKDCQICHIGGFCSGGCKLSADIDFVKQCEMEKRNLKSLWRPF"
ORIGIN
        1 caagaagaag aattgttaaa tggtggcgaa attttacaag aaacacgtcg ttttgatgaa
       61 tccacaggta aaacgatttt aatgcgtgtt aaggaagcgt cagatgacta ccgttacttc
      121 cctgaacctg acattgtacc tttatatatc gatgaagcat ggaaagcgcg tgttcgtgaa
      181 tcgattcctg aattaccaga tgcacgtaaa gaaaaatacg taaaagaatt cggtttacct
      241 gcatatgatg cacatgtttt aacattaaca aaagagatgt cagatttctt tgaagcggct
      301 gttgaagaag gcgcagatgt gaaaatgatt tcgaactggt taatgggtgg cgtaaacgaa
      361 tatctcaata aaaatcaaat tgagttacaa gatacaggcc ttacaccagc aaacttagca
      421 ggtatgatta aattaattga agatggcaca atgagtagta aaattgcgaa aaaagtgttc
      481 ccagaattag cgcaaaatgg tggggacgca aagcaaatta tgaaagataa aggtcttgtt
      541 caaatttctg atgaaggtgc tgtgttagaa tttgtacaac aagcaattgc tgaaaatcca
      601 caatctgttg aagattataa gaatgggaaa ggtaaggcga tgggcttctt agttggacaa
      661 attatgaaat tgtcgaaagg tcaagcgaat ccacagcttg caaatcagtt gttgaagcaa
      721 gagttagata aacaataggt tgttttgaat agaggcgagt agatggaatg caatggtgat
      781 gattgtattt cacactcgtc ttttttgtga cattttgttt tttgagtatg acgtaagatg
      841 tcgtattgat tataacacct tgcaatcgca gaactagggc tcgctttcat aatgtgaata
      901 tctgtatgga ttgcctttta gggtttgctt tcctagggct gttcccaagt aatcaacgct
      961 taactgaaac tgttataagg tggaggcgtt gatggatttc cggcgcatct tttccctcag
     1021 gagtcgcgcc ctgaatggta atcagttgcg taacaaggaa tgaaagcgag cttgttctag
     1081 caatcttagt tgcggtgctc aaacgagttg aaatgacgaa taactcttac caaacagacg
     1141 gcgcatgtat taatcatacg catgcatttt ttcactcatt ttatttatat gattattaat
     1201 tgatcttagg tgtagtggtg aaagaatgat gaagtaactc aatttctttg cttggctagg
     1261 aaagttgaaa tgtcaacata tgacgcaatc gccttttgaa aacgggattg acgttatttt
     1321 cttatcattt ttacgctttt ggttgttttt agctttcatt acatctttgt ttactataat
     1381 ataagttgtg agtactgaaa aattgaggga tataaatgag aaaacgtgcg agaataatct
     1441 ataatcctac atcgggtaag gaagtgttta aacgtgcatt acctgatgta ctaattaaaa
     1501 tggaacaagc tggttatgag acgagtgcct atgcgactca aaaggttggc gatgcgacag
     1561 aagaggcggc gcgtgcgatt gaggcccaat acgatttgtt aattgtcgct ggtggagacg
     1621 gtacattgaa tgaagtcgtg aatggtattg ctgaaaaacc gaatcgtcct aagttaggtt
     1681 taattccaat ggggacggtg aatgatttcg gtcgagcact acatctccca actgatattt
     1741 ttgaagccgt ggatgtcatt ttagatggta aaactgttca agttgatatt ggtaaaatga
     1801 atagccgtta ttttattaat ttagctggcg gtggtaaaat tacggaagtt tcgtatgaag
     1861 caccgagtaa attaaaatcg atagtcggac cttttgcgta ctatattaaa ggatttgaaa
     1921 tgttacctca aatgcatgca gtcgatgtgc ggattgagtt tgattcgcaa gtctttgaag
     1981 gtgaaatcat gttgttctta ctcggcttaa ccaattcaat ggctggtttt gagaagttag
     2041 taccagatgc gaagttggat gacggtatgt ttacactgtt gattgttgaa aaggcaaata
     2101 ttgcagaatt gggacatatt atgacacttg cctcacgcgg tgaacatatt aaacatccga
     2161 aagtacatta ccataaagcg caatcggtaa atatttcttc ctttagcgat atgccgttaa
     2221 acgtagatgg tgaatatggg ggtcaattac cagcgaactt tttaaattta gttcgtcata
     2281 ttgaagtttt ttctccggcg caagaagata atgcgttgtt aattgatgag cctacgcaat
     2341 ctgagtaacg atccagaggt gtgctgaaaa taaataggta gtgtacgcaa aaccgcatga
     2401 tgtaatgaca ttactctcgt gggagcggga cgatgaaatt tgactcagat gttaagtttt
     2461 tggtcctgct cctttccctt tgaagtagat tctgtttata aaaaagtgag atgaaattga
     2521 tggttttagt aaagaaaaat gaagtgtatg agggaaaagt ggttgatttg acacatgaag
     2581 ggcatggtgt gattaaacaa gatcgctatc cgatatttat ccctcaaacg ttaattgatg
     2641 aagaagtgca atataaagtg attaaagtga aaaagaattt cgcgattggt aagctgatga
     2701 atgtgaaaac ggcaagtcca gatcgtgtta caccgccatg tgattattac caacagtgtg
     2761 ggggatgcca acttcaacat ttgtcgtatc gtgcgcaatt agagatgaag cgtaaacaag
     2821 tgattaattt gtttcatcat aaaggaaaaa tgacagaggt gccgattcat gacacgattg
     2881 gaatggatga tccttggcgt tatcgaaaca agtctcaaat tccggttggg acgaatcgca
     2941 atggtcagat ccaaatgggc ttttatcgtc aacgcagcca tgatattatc gatatggacc
     3001 attgtctcat acaaaatgat gatcaaaacg acatgatgaa tgtgattaaa agtttactgg
     3061 aaaaatatca cattccagct tataacgaac acatacatca aggtctcgta cgtcatgtcg
     3121 tgattcgtaa aggctattat acgaatgaag tgatggttat ttttgtgctc aacggtaaaa
     3181 agttaccaca tcataacgat attgtgaccg ctctaacatc acagtttcag caaattgtga
     3241 gtatcaagtt gaatttccaa acgaacaaaa cgaatgtcat tatggggcat acgtccaaaa
     3301 cgattttcgg taaagacatt attgaggata cattagatga tttgactttc gaaattggcg
     3361 atttgtcctt ttatcaaatc aatgtgacac aaacgcaaaa gttgtatcgt caagcgttac
     3421 agtatgcgaa tttaacgggt aatgaagtcg tattagatgc gtattgtggt attgggacga
     3481 tcagtttatt tatggcaccc cacgcccaac atgtatatgg tgtagaagtt gtccctgaag
     3541 cgatagaaga tgcgaaaaga aatgcgcgct acaatcaatt gaacaataca acttttgtcg
     3601 ctggtgctgc tgaagacgtg attttaaaat ggcaacaaga cgggattcag cctgatgtgg
     3661 tgacagttga cccaccgcgc aaagggtgtg acgcgacatt tattgaaact ttaaacgcgt
     3721 tagccccaaa acgtatcgtc tatgtgtcat gcaatccaag tacacaatta cgagatattg
     3781 aaatgctaag cgcgcaatat gaactaaaag aaatgacacc tgtcgatatg tttccgcata
     3841 cgacacatgt cgagacagtt gcgttattag taagaaaata ttgatataga ttaatcactc
     3901 aagggttttt tgagaacatt tttgagaatg tgacttaaaa cccttttttg ttttggtgct
     3961 attttaggag cgttgatact ggaactgatt tgacaaatag gtttgtcgtt aatagacatt
     4021 agctttttta aagtattgtt tagaaacaca gttgtgaaat tgtaaaaata ttgagattag
     4081 acattttgtg tttattaata tctttctaga attaatattc agaaaatttg gaaaaatagt
     4141 ttacatttgt gtatatttat gctacattca tgatggaata ttaagaggag gtgccattat
     4201 gggtggtatt aattgtggac tttccaataa tagagtcgta aagaatgata tgtatcgatt
     4261 agaatatgct ttatacacaa aatatgtgtt taaatgtttt gaaaattttt gtaatgcaaa
     4321 tacatgggtt gaagagttta cacttttgga aattgatgat aatgataacg cactagttgc
     4381 ttataaaaaa gcattatttg ccattcaaga ggcgattact aatttacaga gtgctaaaaa
     4441 tgcactaatc aatagttaca atgagattgc gcctttattt aaatttagtc aattaaaaaa
     4501 tggttcaata aaccaatata aaaagtatga ccattccttt gctaaagggg tatttgaaaa
     4561 ggcaggagtt gtgacgaacg agacaaaggt ttgggagtca gttattcatt ctctgcaaaa
     4621 tggaggagaa gtagattatt taaatgatca acaaaataag ttgattgttt ttgaaaatac
     4681 tttgcaaaat ctatttgagg aatatcaaaa attagaaaag tatatacgac aaggtgtatc
     4741 acatgttgca ataagagata ttgaagttga tattacgccg ttaactgcaa tggccttgac
     4801 taaaatcagt gaattgatga gttcattaac atacttatgt ttagttgaat attcagccca
     4861 tacaagtatc actggcaaaa cgattgatgt attggattta ctaccaaaaa caaacaaaaa
     4921 tagtatacag catctaaaag caaattgatt tttatatact gtgtctatac ggtacagttt
     4981 ttttatgtag ggggattaat atgttagaga atgtttctga acatggggtt tcaggatttt
     5041 acacatatca ctttacagag ggaaactcat ttagaccatg tgctacaaga attattgaaa
     5101 aggaagctat aaaagctact aaagatgagt ttatggagtt aatgcttatt tcaagtaaat
     5161 ctttcccgtt tgtatttttc ggagggaaat ccttaggtaa ttttaaacta cccattcatc
     5221 ttcatccgtt tatgtggcaa aaacgaacta tccaagatgt gaattggtat aatgaaaaga
     5281 atgttgagta tatccctttt tataactaca aagacggatc tttgaagtat ataccgaaag
     5341 aattaagcgt ctattatgaa aaagcgaaag ggattgggct atcttttagt cctcaagagg
     5401 gttattccta tgatcgagat attgtacttg ctaaagtcaa ggcggtcatg gaattaatag
     5461 aaaaagatat tgttactttg tggtggcata gagaaacatc aagtagaatt attgtgattg
     5521 atgatgatac tgatgagaat ataaaaatta ttagaaatca attattaaat caaggtatag
     5581 atatcacgtt attagatatt gaaaatgatt taggtgttta tgtggttgtg tgtatcctca
     5641 agcaagatga ctttccatca atcacttatg gtagtgcagc gcattttgaa attaaaggtg
     5701 cagttaaaca tgcaattttt gaagcgattt catgtattgt gggtttgagg tatgagttca
     5761 ttcattttaa aaaaatccgt actagtttta cgtatcctga ttttattagt gatagtccat
     5821 taactaattt atccgattat aaagagatat tgaacttaaa aacagccatt aataaatatg
     5881 atatatatta ttcttatatt tctgctgatg aaggatactt agtgaaagcg tactgttatg
     5941 aattacaacc caccttgtat agtgcaacgg ttcctttaac taaacgtttc tttagagtaa
     6001 acaagaagga agtcaggatt aaagaagatt ttccgttcat atagaaggtg agttatgaaa
     6061 actaaaagaa ttattgcgtc agatttttta acaacgatga tgtcttcagc cttaacgatt
     6121 tatatatatt ggtttgctta tcaatacttc tctagtcaac tcatcatatc aatcatcggt
     6181 tttgggcaat taagtggtgt gtttctctca agtcttggtg gtggcatctc agacagtatg
     6241 aacaagctgt ttttcatcaa aactctgaac ttctttaaag tcggattact ttttatggtg
     6301 ttgatattag agaacgttgt gaacatggaa attttgttgc cactttttat gtttgcttca
     6361 acagtgatag gaggtttaca gtcacctacg ttagaatcat tggttccatt tttagccagt
     6421 agcgatgagg cgctttttcg tattaacgct gtggtttcga gttcaacaca ggttgcatct
     6481 attatggcga ttgttttatc tgcagtctat atttcatttc tgtcttttgt aacgattgtt
     6541 tttgtgaccc tttttatcac tgccatcgca acgattttac ttgtaggact gacggtggaa
     6601 acgccattga agtctacttc agttgttaat aatatgaagc atggggcgag ttatatcttt
     6661 aaaacgcctt atattcgtaa ccttattcct gtcgcattgg tcatgaattt ctcgttttgg
     6721 agtattttct tgctcttacc taaaatcgct aatgatagtt tttcattttt aaaaacctct
     6781 tatagtggca tggagctctc ctttgcttta ggagcgggct taggcgggat cttatttgcg
     6841 aaattcttct atgatgttaa agataaatat cgtttattta agaggagttt gttttcacag
     6901 agttatgttt tgttgttatt aggaatcgtt ttacttattc ctaacagtgt tcttgcctat
     6961 accgggatgc tgttgtgctg gtttttgtat gctttgatca acacgatttt ctctattctc
     7021 tattttggaa agattcaatt gaatgtccct agagaaatga ttggcagtgt ttttggagca
     7081 attttaacgc tcttttcatt agtcaatccc atcgcagcca tcatgtcggg agttctcgtt
     7141 tcgcatttta aaatacctct gttaattata ttgttgtctt cactcatgtt gttagctgcc
     7201 tggagtattc gtttcctagc agatgttgaa acggtatttg attaaagggg ggagggatat
     7261 gtctatatta acacttaaaa acatcaggtg tgagatggat gggaaattat tatttgaagc
     7321 ggatggttta acgattgagg aaggcgatgt cattggcctt atcggaaaga acggtgctgg
     7381 aaagacgtgt ttgttaaaaa tattagcagg agagttaagc gattatgctg gctatatatc
     7441 gggcaatcgt ctgacttatt ttagtgaact ctgtataacg gaggatatga ctcaaagtgg
     7501 tggagaacta agcatttcaa gttttctcaa cgcattaaga cagtcggtgc ccctctattt
     7561 gttggacgaa cctacaacgt atttagatca acatcatttg aagaaagtag taagaacgat
     7621 tagacgctcc ccatcttctt tttgtatagc gagccatgat cgggcttttt tagatgcagt
     7681 tgcgactaaa atatggttgt tagagcaagg aaaaataaca gaatataatg gttctttttc
     7741 agattatgtg ttagcacgtt caatgcagtt atcacaatat gagcaagagt tgaaacaata
     7801 tcagaaggaa aaaaagaaaa taaaacaatc acttcgaatg atgaaagaaa agcaagaaca
     7861 gaaaaaggga aaaccaaaga agatgagtac ttcagaatat cgtattgcgg gtatcaaaac
     7921 taagtacggt gtgaaacaaa aaaagttaca gaagcgtatt acacgacaac aaaaaaagtt
     7981 agaacagtta aaacaaccat atcaagttga ggacaaatat gatatttcat ttttatcaca
     8041 acttcattgt ttacccaaaa gaaaaattat cattcctagt cacgaagcga aaatagggca
     8101 aaaaagcttg tggcataccc caactatcca tttaaatagt ggtgataaat taggaattat
     8161 tggaagaaat ggtgcaggca aaacaactta tttgaattac ttagtttcag ttttaccttc
     8221 tacatataaa atttgttatt ttcgccaaaa tcattttcag tacttagacc caaagatgac
     8281 agtatatgaa gcgattgtcg aagctacgaa tgggtattta agcgaacatc acattagaac
     8341 tttgttggcg ttattagatt ttaaaagaga caaggtattt cgattaatag gagatttaag
     8401 ccgaggggag cacgtgaagg tttcattatt atctctctta gtttctgagt ctgatatttt
     8461 gattttagat gaaatgacca actttttaga tataaaggct atcgaagcag tggaaaaggt
     8521 gcttgctaca tattcaggca ttctgatatt tgtgagtcat gatcagtatt ttgtacaacg
     8581 tttagctact tcgattctag atattgatac ctataagata atgtaggatg gacaattaca
     8641 aatattttgg agtgaaagaa tctccctcca tcaagcatct atcaaatagt gatagtataa
     8701 aaaggacgtc cgtttgatta ggtaccctgt tttgatgaca gtttaacaaa aacaataaaa
     8761 gttttaaggt acagactact agctgtaatg ctatattatt gacgcagaga tgtgatgttc
     8821 tcatttttaa aagttgtgta gcctccgtga tcaagttaaa ggtgagtgtt caatatccat
     8881 tattaaagaa agttatttta tttttggaag gtaaaccaat gtcatcaaaa ataaaaatga
     8941 aaccatgcaa tgataattcg taatttctgt gtgaaacgta ggtaacatat gaaaaatcac
     9001 gctataaatt tcagaataat aagaaattac aaaggggtta cgcttaagga aatttctaaa
     9061 agagggatat catcattcca actctcacgt tttgataaag gggagactca cgactgcaag
     9121 aatgctacag tagttgattt ttcgaatcag cgtagaatga tgtggtgaca gttggcccac
     9181 cgcgcaaagg gtgtgacgcg acatttattg aaactttaaa cgcgttagcc ccaaaacgta
     9241 tcgtctatgt gtcatgcaat ccaagtacac aattacgaga tattgaaatg ctaagcgcgc
     9301 aatatgaact aaaagaaatg acacctgtcg atatgtttcc gcatacgaca catgtcgaga
     9361 cagttgcgtt attagtaaga aaatattgat atagattaat cactcaaggg ttttttgaga
     9421 acatttttga gaatgtgact tagaaccctt ttttgttttg gtgctatttt aggagcgttg
     9481 atactggaac tgatttgaca gtgttaatct ctagagaatg gcgagtaatg aagttttgaa
     9541 gttattctaa atataaaata aaatcattgt tctgaatatt caaaatacac atttacatat
     9601 tctgtattca tacgctatat taaagatata agattaagaa atggaatttg attatagatt
     9661 acgtgaatgg ttggatacac cttcttctaa taaaaatcaa ttttcttctg atacacttta
     9721 taatgaactt ttgttatcag agtcaaaaaa gaaaatacaa atatctttag caattactta
     9781 ttcttgtaat ttaagatgta gttattgttt tcagcagaac tatgatcatc tcgttagaaa
     9841 accaataact atagaagcac ttgaaaaaat tttagataag atttcattaa tcctttatca
     9901 aaatccagaa gttgatgttt ctattgggtt atttggtggg gaaccactat tgccacaaaa
     9961 tgaaaaaatt attgatagag tttttcaata ttgtgttgaa aacaaattga agatagacat
    10021 tactactaac ggaagctttt tgccttattt tgccaaaaag atagtgatac atagaagtat
    10081 tatatctgtt atagcaatta ctattaatac gttaccggac aaatataatg agacaattaa
    10141 aataacgaaa gttgcaaaca atgttgagga attattagct gttactgatt tattattaca
    10201 ctatcatata gttgtagatg tggggacaaa ttttgataga aataatttaa gtgaactgtt
    10261 gccaatctat cactattttt tcgacaaagg gtatttttca aagtggaatt ttcattggaa
    10321 tatcggaagg gttgacgatc gcctttttga tactggttat gatgaatata ttgtttctga
    10381 gactgatata cttctacaat taatgaaaat acctaaacca gtaccatcaa accttcatgc
    10441 aggctttatt caaacgtgca agaacttaac tgataaattg aacttgtctt ttaatgaatc
    10501 tcaaattaaa ggcacatatc attactgctg gaacgtgtct ccctatgata gagttttcta
    10561 tattgacaat gaacttaatt tgtttagatg tactgtaaca gtagggcgcc ctcaatatat
    10621 tttaggaaac ttaaaagata ttgatttaat gaactaccaa catgaaacaa aaagcttctt
    10681 agattataaa gattgtcaga tttgtcatat aggcggtttt tgttctggtg ggtgtaagtt
    10741 atctgctgac attgattttg ttaaacaatg tgagatggaa aaaaggaatt tgaaaagttt
    10801 gtggaggcca ttttaattcc acaagtaagg gagaaactga acagaaatga ctaaggcaat
    10861 caaacaatta ttgatttctc aagcagttag taatttagct gatgtgtttc ttagagttgt
    10921 agtcattgca aatgtattca ccatcacaaa gtcagtcatt gcgacatcaa tggtgcctat
    10981 tctgataggt ttatcttctt ttattgcaag ttttttagtc cctttagtta ccaaaaaaat
    11041 gtta
//
