LOCUS       NZ_CP066713            11866 bp    DNA     circular CON 14-APR-2024
DEFINITION  Staphylococcus pseudintermedius strain DSP026 chromosome, complete
            genome.
ACCESSION   NZ_CP066713
VERSION     NZ_CP066713.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 2567628)
  AUTHORS   Ferrer,L., Perez,D., Fonticoba,R., Vines,J., Fabregas,N.,
            Madronero,S., Meroni,G., Martino,P., Martinez,S., Cusco,A.,
            Migura,L. and Francino,O.
  TITLE     Whole genome sequencing and de novo assembly of Staphylococcus
            pseudintermedius: a novel pangenome approach to unravel pathogenesis
            of canine pyoderma
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2567628)
  AUTHORS   Ferrer,L., Perez,D., Fonticoba,R., Vines,J., Fabregas,N.,
            Madronero,S., Cusco,A., Migura,L. and Francino,O.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2020) Servei Veterinari de Genetica Molecular,
            Universitat Autonoma de Barcelona, Edifici V, Travessera dels
            Turons, Bellaterra, Barcelona 08193, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUL-2020
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 303x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016455085.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:32:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,471
            CDSs (total)                      :: 2,390
            Genes (coding)                    :: 2,301
            CDSs (with protein)               :: 2,301
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 63 of 89
            Pseudo Genes (incomplete)         :: 28 of 89
            Pseudo Genes (internal stop)      :: 9 of 89
            Pseudo Genes (multiple problems)  :: 10 of 89
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:05:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2081194
            Orig. end                         :: >2093060
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066713.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..>11866
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..>11866
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..>11866
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:>6866](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..>6866)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(149..1930)
                     /gene="recQ"
                     /locus_tag="JGZ50_RS09950"
                     /old_locus_tag="JGZ50_09950"
     CDS             complement(149..1930)
                     /EC_number="3.6.4.12"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recQ"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424488.1"
                     /locus_tag="JGZ50_RS09950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_09950"
                     /product="DNA helicase RecQ"
                     /protein_id="WP_015728744.1"
                     /transl_table=11
                     /translation="MEQTLSHYFGYKSFRPGQKEIIERVLNHHHTLGVLPTGGGKSLCY
                     QVPGLMFKGTTIVISPLISLMKDQVDQLTAMGISAAYLNSSLSTQAQKEIETQLVNGEL
                     KFLYIAPERFEQTYFEMLLRRVDISLVAFDEAHCISKWGHDFRPSYQAVIQRVLTMPQQ
                     FSIVALTATATTEVQQDIMERLMISRQHLVKTSIRRRNLKFQVNGTYQRQKFVLDYIQK
                     HSQKAGIVYCSTRKQVEALYEALEDADIPVSYYHAGMTAKQRDEAQNDFLYDRTRVVVA
                     TNAFGMGIDKSNVRYVIHYNMPGDLESYYQEAGRAGRDGLDSDCILLYSDRDIALHQYF
                     IGASKADDDYKERMGEKLTKMIQYTKTTKCLEATLVHYFEPNEKLEECHQCSNCIDKNK
                     TYNMTKEAKMIISGVARLRQQEGYQTVIQVLRGEVSDYIRHQGYENLSTYGLMKDYTTG
                     ELHHLIDELRFKGFLNEHDEILMCDASVEKLLSEDTQVFTRPFKRKSKETVHINTVEGV
                     DRTLFEKLVEVRREMSERLNMPPTNIFTDYTLEAFAKRKPVTKQEMIQIDGVGSYKLKH
                     YCPEFLEAIQEYKMHRS"
     gene            complement(1940..3817)
                     /locus_tag="JGZ50_RS09955"
                     /old_locus_tag="JGZ50_09955"
     CDS             complement(1940..3817)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166146.1"
                     /locus_tag="JGZ50_RS09955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_09955"
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="WP_103863357.1"
                     /transl_table=11
                     /translation="MEAYKIERLNKSYADKVIFDDLQLSISNQERIGLVGINGTGKSSL
                     LKVIAGMDEDFDAVQSHPKQYRIRYAAQNHNLEPTLTVYEAVYSADSKELQVIKRYEEA
                     TAQYAEQQTDKAFDAMMAAQADMDAYSAWDYSAEIKTILSKLGIHDTTRRMSELSGGQQ
                     KRVVLARTLIEKPDLLLLDEPTNHLDFESIHWLIQFVKSYPKTVVFVTHDRYFLNEVAT
                     RIVELRNGKLDTYPGNYEAYIAMRAEREEIEARQQTKQRALFKQELAWMRAGVKARTTK
                     QQARQDRFHDLKEQVQSHQVQGKGSLNLAYSRLGKQVFELENVTKRIDGRTLFENVSTL
                     IQKGVQIGIVGPNGVGKTTMLNILAGEDQHFEGVLKIGQTVKVAYFKQTDERLNRDVRV
                     IDFLREESELARQKDGTTVSITQLLERFLFPSVTHGKKVYKLSGGEQKRLYLLKLLVHQ
                     PNVLLLDEPTNDLDTETLTILEDYIQTFGGTVITVSHDRYFLDKVVDCYWYIHDGQIDM
                     ILGQFEDYLAYQKQLEQAATQVASVKQQPTLQPKKKKGLSYNEKREYDMLLVRIEETES
                     RLAEIDAEMIEANADYAKIKALNEEKETLEAQYDTDMTRWSELEEILEQ"
     gene            3929..4744
                     /locus_tag="JGZ50_RS09960"
                     /old_locus_tag="JGZ50_09960"
     CDS             3929..4744
                     /GO_function="GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019168536.1"
                     /locus_tag="JGZ50_RS09960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_09960"
                     /product="ZIP family metal transporter"
                     /protein_id="WP_015728742.1"
                     /transl_table=11
                     /translation="MLDYISNMPPFLQALLAGVITWLLTALGAATVFVFKSVNEKVLNS
                     MQGFAAGIMIAASFWSLLQPAIESSSGSTVPWLPAAIGFLLGGVFIRSLDYVIPHMHRN
                     ATDESQHQEGVETSLNKNTLLLLAITLHNIPEGLAIGVAFGGIATGNEHATLLGALGLA
                     IGIGIQNIPEGAALSLPIHASGQSKWKAFNYGQASALVEPVFATVGAAAVLVVTPILPY
                     ALAFAAGAMIFVVVEELIPDSQAGKNTDLATLSLMVGFTIMMILDVALG"
     gene            complement(5001..>6866)
                     /locus_tag="JGZ50_RS09965"
                     /old_locus_tag="JGZ50_09965"
                     /pseudo=""
     CDS             complement(5001..>6866)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /locus_tag="JGZ50_RS09965"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_09965"
                     /product="peptide cleavage/export ABC transporter"
                     /pseudo=""
                     /sec_met_domain="TIGR01193 (E-value: 6.4e-231, bitscore:
                     765.7, seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_C39 (E-value: 7.1e-10, bitscore:
                     36.0, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="HFHYYVVIRCDKKYIYIADPVVNLTKMTYREFKEEWTGVTILLKP
                     TSLYKPIKQDKVRLTSFIPLLLKEKRLIIKIIIISILVNMINILGSYFMKSIIDFYIPQ
                     KLKTWVSMISMALIASYIMQQILSYLQKYFLILLVKTYSINILSPYIKHLFRLPITFFE
                     TGRAGDILSRLNDANSITDALASTMLSLFLDVTIIVGISIFLYYQSTILFVLILIIIPI
                     YAVIVFGFMNSFERKRIKSMEANATLSSYLMENITGMETIKALTCEKKCYKQIKYNFSN
                     YYEKTFLLNKLNIKQNVLKKVVQLILNVSILWVGANLVIDNQISIGTLLVFNSLMIYFM
                     NPIENIMNLQLKLQIAGVANNRLNEIFTVKSEFEKNKLDIKLNQNSSKLIFSNISYGYV
                     YEKEILTDINITIEHNSKVAFIGESGSGKTTLAKLMVSFFEPSKGKITLGGLDITNINK
                     SVLRQQIIYIPQQTYIFNGTILENLLLGVVKEVTQKDIDSAIKMVELKSFIDSLPLGLD
                     SVLSSDFSALSGGQRQKIALARALLTNSSILIMDEATSNIDILTERKIINNLMALNKTI
                     IFISHNLNITQKVDKIFVLAKGKIIEEGSHEFLLKAKGYYSNLIN"
     gene            complement(6845..7042)
                     /locus_tag="JGZ50_RS12625"
                     /old_locus_tag="JGZ50_09970"
     CDS             complement(6845..7042)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /locus_tag="JGZ50_RS12625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_09970"
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_197737723.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.2e-11, bitscore:
                     39.7, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFLRRKLRTTVNGTTAYGLVQVADKLGFETVPIKADISLFEIDD
                     LQYPFIVHVKKITIIFTIML"
     gene            complement(6966..7157)
                     /locus_tag="JGZ50_RS12630"
                     /old_locus_tag="JGZ50_09975"
     CDS             complement(6966..7157)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /locus_tag="JGZ50_RS12630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_09975"
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_197737724.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.7e-08, bitscore:
                     29.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFSKRFYRAQVDSQDCGAAALAMILEFYGSHYSFSLFFGFSEKK
                     VKNNSKWHYSIWFGSGSR"
     misc_feature    7336..7349
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: strong, score: 21.85"
                     /tool="antismash"
     misc_feature    7363..7378
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 24.05"
                     /tool="antismash"
     gene            complement(7532..7840)
                     /locus_tag="JGZ50_RS09980"
                     /old_locus_tag="JGZ50_09980"
     CDS             complement(7532..7840)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024424.4"
                     /locus_tag="JGZ50_RS09980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_09980"
                     /product="DUF3884 family protein"
                     /protein_id="WP_063284913.1"
                     /transl_table=11
                     /translation="MIKISLKKILCQLSQKKLYEKTFQSIYIVDFSLLDRAPLFKDEFK
                     VIGTWYSYSGKRWICHTELSTEQFKKMITKNIDHKDLEKVKFYLDYLPFSITNEIPF"
     gene            complement(7915..8277)
                     /locus_tag="JGZ50_RS09985"
                     /old_locus_tag="JGZ50_09985"
     CDS             complement(7915..8277)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JGZ50_RS09985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JGZ50_09985"
                     /product="hypothetical protein"
                     /protein_id="WP_131090790.1"
                     /transl_table=11
                     /translation="MFKLLEESNHERFTYYMELLEKDFNHTQDFIFLKYSYYIKKLNID
                     ATNLLKSFLENYDLLNEGDVIFSKECTHSLLGASLFWGGYLKESKKVFEELEINSKDTV
                     CFYNKKFIYDCLSTLS"
     gene            complement(8334..9044)
                     /locus_tag="JGZ50_RS09990"
                     /old_locus_tag="JGZ50_09990"
     CDS             complement(8334..9044)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /locus_tag="JGZ50_RS09990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_09990"
                     /product="glycosyltransferase"
                     /protein_id="WP_199274729.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.2e-18,
                     bitscore: 64.7, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEKINKLEIISEYTTISVVYIVKNERDYIIKSLLNILDLADEII
                     IVDTGSEDNTLDLIKKMCDKKIKIFTFNWCDDFSKARNFANAKATCDWIMILDADEIVR
                     KNDNLKFYLTYLRIFDDFENTAFNFKVIRDEEVYKTSKLIRNTNTLTYKGRVHETFFSL
                     NNSVSYANLNVEVLGIRRGSQKKTNYYNKLLLKTIIDFPKEGRWYYLYLRYNIEDINYN
                     KMLKFCKKLYLKEM"
     misc_feature    10187..10200
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.13"
                     /tool="antismash"
ORIGIN
        1 attcattgta aaaataacac aatttgcatc taaaaaattt aaatcatcta ctatcgacac
       61 gtctcacttt tccattcttt accactcaat aagaaatcgt ttatttgtaa aaaaggagcc
      121 aggaattccc ccagctccat ctgatgtttt acgaacgatg cattttatat tcttgaatcg
      181 cttctaaaaa ttcaggacaa tagtgcttca gcttataact accaacacca tcaatctgaa
      241 tcatttcttg ctttgtgaca ggctttcttt tcgcaaaagc ttctaacgta tagtctgtaa
      301 aaatattcgt cggtggcatg tttaatcgtt cgctcatttc acgtctgact tcgacaagct
      361 tttcaaataa cgtgcggtcg accccttcta cagtgttaat atgcactgtt tctttcgact
      421 tacgcttgaa cggccttgta aacacttgtg tatcctcact caataacttt tctactgagg
      481 catcacacat gagtatttcg tcatgttcgt tgagaaagcc tttaaagcgg agttcatcga
      541 ttaaatgatg taactcgcca gtcgtatagt ctttcatcaa accatacgtc gaaagatttt
      601 catagccttg atgacgaatg tagtccgaaa cctcgccacg caaaacttga atgaccgttt
      661 gatatccttc ttgttgacgt aaacgtgcga caccgctaat gatcattttc gcttctttcg
      721 tcatattgta cgttttattt ttatcaatac aattactaca ttggtgacat tcttcaagtt
      781 tttcattcgg ctcaaaataa tggactaatg ttgcttctag acatttcgtc gttttcgtat
      841 attgaatcat tttcgtcaat ttttcgccca tacgttcctt gtaatcatcg tccgctttcg
      901 aggcaccgat aaaatactga tgtaaagcaa tatctcgatc actgtacaat aaaatacaat
      961 cactatctaa tccgtctcga cctgcacgtc ctgcttcttg ataatacgat tccaaatcac
     1021 ccggcatatt gtaatgaatg acataacgga cattcgattt atcaattccc ataccgaatg
     1081 cgttggtcgc aacgactaca cgagtacggt catataaaaa gtcattctgg gcttcatctc
     1141 tttgtttcgc tgtcattcct gcatgatagt aggatacagg gatatctgcg tcttctaaag
     1201 cttcatacaa ggcttctact tgtttacgcg ttgaacaata aacaatcccc gctttctgac
     1261 tatgtttttg aatataatcc aatacaaact tttgacgttg atacgtccca ttcacctgga
     1321 acttcaaatt acgtcgacga atactagttt tgactaagtg ttgtcgcgaa atcattaacc
     1381 gttccataat atcttgttgc acttcagtcg tcgctgttgc agtcaatgcg acaatcgaaa
     1441 actgttgcgg catagtcaat acgcgttgta tgactgcttg ataactcggc cgaaaatcat
     1501 gtccccattt tgaaatacag tgtgcttcgt caaatgcgac gagtgaaata tcgacacgtc
     1561 gcaacaacat ttcaaaataa gtttgctcaa aacgttccgg tgcgatgtat aaaaatttca
     1621 actcaccatt gaccaattgc gtttctatct ctttttgtgc ttgcgtactt aaactactat
     1681 tcaaataagc cgcagatatt cccattgcgg tcaattggtc cacttggtct ttcattaacg
     1741 aaattaaagg actaatgaca atggtcgttc ctttaaacat caatcccggt acttgataac
     1801 ataacgattt acccccacct gtgggtaaca cccctaacgt atgatgatga ttcaatacgc
     1861 gttcaataat ttctttttga ccaggacgaa aagatttgta tccaaaataa tgcgacaatg
     1921 tttgctccat gctatccctc tattgttcta atatttcttc tagttcactc caccgcgtca
     1981 tgtcggtatc atattgagct tcgagcgttt ctttttcttc atttaatgct ttgattttgg
     2041 cataatctgc gttcgcttcg atcatttcgg cgtcaatttc agccagacgc gactctgttt
     2101 cctcgatgcg cactaataac atgtcatatt ctcgtttttc gttgtatgat aatccttttt
     2161 tcttttttgg ttgtaatgtc ggttgttgtt tcacggacgc tacttgagtt gctgcttgtt
     2221 ccaattgttt ttggtatgct aaataatctt caaactgccc taaaatcatg tcaatttgac
     2281 cgtcgtgaat ataccaataa caatccacga ctttatctaa aaagtaacgg tcatggctca
     2341 cagtaatgac ggtaccgcca aatgtttgaa tgtagtcttc taaaatcgtt aacgtttctg
     2401 tatccaaatc atttgtcggt tcgtctaaca acaacacgtt cggctgatgc actaacaact
     2461 tcaataaata caaacgcttt tgttcgccac ctgagagttt atacactttc ttaccatgcg
     2521 tcacactggg gaataaaaag cgttccaaca attgcgtaat cgaaacggtc gtcccatctt
     2581 tttgacgcgc caactcactt tcttcacgca agaaatcaat gacacggaca tcacgattca
     2641 atcgttcatc tgtttgttta aaatatgcca cttttaccgt ttgacctatc tttaatacac
     2701 cttcaaaatg ctgatcttca cctgctaaaa tgttcagcat cgtcgttttg ccgacaccgt
     2761 ttggtcccac aataccgatt tgaacacctt tttgaatcaa tgtcgacacg ttttcaaata
     2821 atgtacggcc atcaatacgt ttcgtcacat tttcaagttc aaagacttgt ttgcctaaac
     2881 gcgaataagc cagattcaat gaccccttcc cttgcacttg atgcgattgt acttgttctt
     2941 ttaagtcatg aaaacgatct tgccttgctt gttgcttcgt agtccgcgct ttgacaccgg
     3001 cacgcatcca cgctaactct tgtttaaaaa gtgcacgttg tttcgtttgt tgacgcgctt
     3061 cgatttcttc ccgctctgct cgcattgcaa tatacgcttc atagttacct ggatatgtat
     3121 ctagcttgcc atttcgcaat tctacaatac gcgtcgccac ttcatttaaa aagtaacggt
     3181 catgcgtgac aaaaacgact gttttcggat aagactttac aaattgaatt aaccaatgaa
     3241 tcgattcgaa atctaaatgg ttcgtcggtt cgtctaataa taataaatct ggtttttcaa
     3301 tcaacgtacg cgctaacact acacgctttt gttggccacc tgagagttca ctcattcggc
     3361 gtgtcgtatc atggatgcca agtttcgata aaatcgtttt aatttcagca ctataatccc
     3421 aagcactgta agcatccata tcagcttgag ctgccatcat tgcatcaaac gctttgtcag
     3481 tctgttgttc tgcgtattgt gcagtagctt cttcataacg tttaatgact tgcaactctt
     3541 tggaatctgc actataaacc gcttcataaa ccgttaacgt tggctccaaa ttatgatttt
     3601 gtgccgcata acgaatacga tattgtttcg gatgcgattg aacggcatca aaatcttcat
     3661 ccatgcctgc gatgactttg agcaatgaac ttttccccgt tccattaata ccaactaatc
     3721 cgattcgctc ttgattggaa atggataact gtaaatcatc aaaaatgact ttatccgcat
     3781 aagatttgtt gaggcgttca attttatacg cttccacaac acatacaccc tttttcttat
     3841 agatttactc gcatttatct gttaacctta aatcagttct atatattata cacttttttt
     3901 ggaacaaatg atacaaggag ctatttctat gttggattat atttcaaaca tgccgccctt
     3961 tttacaagca ttacttgcag gtgtcatcac gtggttatta accgcattag gggctgcaac
     4021 cgtatttgta tttaaaagtg tcaacgaaaa agtgttgaat tcgatgcaag gctttgctgc
     4081 tggtattatg atcgccgcaa gtttttggtc attactccaa ccggccattg aaagcagttc
     4141 gggcagtact gtaccgtggc tccctgctgc aattggcttt ttactcggcg gggtattcat
     4201 ccgttcttta gactatgtta ttccacacat gcatcgaaac gcaactgatg aatcacaaca
     4261 tcaagaaggt gttgagacga gtctaaataa aaacacactt ttattacttg cgattacatt
     4321 gcataacatt cctgaagggc tcgcaatcgg ggttgccttt ggtggtattg cgacaggaaa
     4381 tgaacacgca actttgttag gggcactcgg acttgcaatt gggattggga ttcaaaacat
     4441 tcctgaaggg gctgcattat cattaccgat acatgcttca ggacagtcca aatggaaagc
     4501 cttcaactat ggacaagctt cagcacttgt agaacctgtt tttgctacag tgggcgcagc
     4561 tgctgtgttg gtcgttactc cgatactacc atacgcactc gcttttgctg caggggcgat
     4621 gatttttgtc gtcgttgaag aattaattcc tgattcacaa gccggtaaaa acacagatct
     4681 tgcgacacta agcttgatgg ttggctttac catcatgatg atattagatg ttgctttagg
     4741 gtaaattcaa aaaaccgttc aatgtgatga aacactgagc ggttttttga tgtgtacata
     4801 tatcgataca ttaaaaatac tctttaacaa ctcacaacaa agatttcaca ctgttgacta
     4861 tataaaatat caaccaatat accaaaatat aataattaac ttaagttata attgtaaaat
     4921 tcagaaaata aaaggtgtga aaaaacagta ccttcatttc gttcttcaca ttggttgtgt
     4981 taatacaaaa aataccatta ttaattaatt aagttagaat agtaaccttt tgcttttaat
     5041 aaaaattcat gtgagccctc ttctataatt tttccttttg ctaaaacaaa tattttatct
     5101 actttttgag ttatatttag attatgggag ataaatataa tagttttatt taacgccatc
     5161 aaattattaa taatttttct ctccgtgagt atatcaatat tactcgttgc ttcatccatt
     5221 atcaatatag aagaatttgt taatagtgct cttgctaaag ctatcttctg ccgctgacct
     5281 cctgacaaag cagaaaaatc agaacttaat acactatcaa gtccaagtgg taagctatca
     5341 ataaaggatt ttagttcaac cattttaata gcactatcta tgtccttttg cgtaacctct
     5401 ttaactaccc ctaataaaag attttcaagt atagttccat taaaaatata agtttgttgt
     5461 ggaatgtata taatctgctg tcgtaaaaca gatttattga tatttgttat gtccaatcca
     5521 cctagtgtta ttttaccctt tgaaggttca aaaaaactaa ccatcaattt cgccaaagtt
     5581 gttttacctg atccagactc tccaataaag gctactttac tattatgttc tattgttata
     5641 ttaatatctg taaggatttc tttttcgtac acatatccat atgaaatatt tgaaaatata
     5701 agtttacttg agttttggtt caatttgata tccaatttat tcttttcaaa ttctgattta
     5761 actgtgaata tttcattcaa tcgattatta gccacacctg ctatttgtaa tttaagctgt
     5821 aaattcataa tattttctat tggattcata aagtaaatca ttaatgaatt aaaaacaagc
     5881 aaagttccaa ttgagatttg attatctata actaggttag cgcctaccca taaaatcgat
     5941 acatttaata ttaattgaac aacctttttc aaaacgtttt gctttatatt tagtttgttt
     6001 aacaagaatg ttttttcgta ataattcgaa aaattatatt ttatctgctt atagcatttc
     6061 ttttcacatg tcagtgcttt tatcgtctcc atacctgtta tattttccat caaataagaa
     6121 gacaaagtag catttgcctc catagatttt attcttttcc gttcaaaaga gttcataaaa
     6181 ccaaaaacaa ttacggcata aataggaatg ataattaata ttaatacaaa taaaatagta
     6241 ctttgataat acaggaagat agatatgcca actattattg taacatctaa aaacaatgat
     6301 agcatggtac tagctaaagc atcagtaatt gaatttgcat cattaagtct tgatagtatg
     6361 tctcctgctc ttcccgtttc aaaaaaagta ataggtaatc taaacaaatg tttaatgtat
     6421 ggtgaaagta tatttataga atatgtcttt actaataaaa tcaaaaaata tttctgcaaa
     6481 taactaagaa tttgttgcat aatgtaagaa gcaattaaag ccatggaaat catactaacc
     6541 catgttttta acttttgggg aatataaaaa tcaataattg acttcataaa atatgaacct
     6601 aaaatattta tcatattgac taaaatggaa atgattatta tttttataat caatcttttt
     6661 tcttttaata ataaaggtat aaaagaagtt aaacgaactt tatcttgctt tataggctta
     6721 tataaagatg taggttttaa taagattgta actccagtcc actcttcttt aaattcccta
     6781 taagtcatct ttgttaaatt aacaacaggg tcagctatat agatatactt tttatcacat
     6841 ctaattacaa cataatagtg aaaatgattg ttattttttt aacatgaacg ataaaaggat
     6901 actgtaaatc atcaatttca aacaaagata tatctgcttt aataggaact gtctcaaaac
     6961 ctaacttatc tgctacctga accaaaccat atgctgtagt gccatttact gttgttctta
     7021 actttcttct cagaaaatcc aaagaatagt gagaaagaat agtgagaacc gtaaaattct
     7081 aatatcatag cgagcgctgc cgcgccacaa tcttgagaat ctacttgtgc acgataaaat
     7141 cgttttgaaa atttcattcg acacttctac tccttcccac cactttttag aacaacattt
     7201 aaatattcaa aaagcgggcg gtaattccca cccacttaat atcatgtcaa aattcaatcc
     7261 tattattaaa agctttaatg gttattcatc tttacatatt tttttcatat aatcacatgc
     7321 actctctgga tcagatgtac cgaatctaac caatatactg tcgtttatac atgtaatctg
     7381 agcccattta caacgggaca tcccaccact taagtctttc agcacctgat ttgatatcac
     7441 tttaaattta tcaatacttt ttatatctat ccctcctaaa tagaacaatt taataatact
     7501 attaaaaatc aattaaatgt atgatttatt ttcaaaacgg aatctcgtta gttatagaaa
     7561 atgggagata atccaaataa aatttgactt tttctaaatc tttatggtct atattttttg
     7621 ttatcatttt cttgaattgc tcggttgata attccgtatg gcatatccaa cgcttaccac
     7681 tatagctata ccaagttcct attactttaa attcgtcttt aaatagagga gccctatcta
     7741 aaagactaaa atcaactata taaatacttt gaaatgtttt ttcataaagt tttttttgag
     7801 ataattgaca taaaattttc tttaaagata ttttaatcat agatttctca ctcctttaca
     7861 ctacctgtta tcttaatgcg gtagattcct gagctataat aatatcaata tttattagct
     7921 taaagtcgat agacaatcat aaataaattt tttattataa aaacaaactg tatctttact
     7981 atttatttct aattcttcaa atactttttt actttccttt aaatatcctc cccaaaataa
     8041 agaggctcct aataacgaat gagtgcattc tttcgaaaaa attacatctc cctcatttaa
     8101 caaatcataa ttctctaaga aacttttcaa taaatttgta gcatctatat taagtttctt
     8161 tatatagtat gaatatttca aaaaaataaa gtcttgagta tgattaaaat ctttctctaa
     8221 cagctccata tagtaagtaa aacgttcatg attggattct tctaataact taaacatcaa
     8281 aagaaaaata attctaacag agtaataatt gttgtttatt tcgattccag atatcacatc
     8341 tcttttaaat ataatttttt acaaaatttt aacattttat tataatttat atcttctata
     8401 ttatacctca aatataaata ataccaacga ccttcttttg ggaaatctat aattgtttta
     8461 agaagcaatt tattataata atttgttttc ttttgacttc ctcttctaat tcctaaaact
     8521 tctacattta aattagcata agatacagaa ttatttaaag aaaagaatgt ttcgtgtact
     8581 ctacctttgt acgtcaaagt attagtattt cttatcagct tacttgtttt atatacttct
     8641 tcatctctaa ttactttaaa attaaaggcc gtattttcaa agtcgtcgaa aattcttaaa
     8701 tacgttaagt aaaactttaa attatcattt tttctaacaa tttcgtcagc atctaaaatc
     8761 ataatccagt cacaagttgc tttagcattt gcaaagtttc ttgcttttga aaaatcatca
     8821 caccaattaa atgtaaatat ttttattttt ttatcacaca tttttttaat taaatctaaa
     8881 gtgttgtctt cagaaccagt gtcaacaata atgatttcat cggctaaatc taaaatattc
     8941 aaaaggcttt ttataatata atctctttcg tttttaacaa tatacactac agaaattgta
     9001 gtatattcac ttataatctc taatttattt attttttcaa tcaattctgc atcgttttta
     9061 actcttttag actcccaatc ttttgattct ataaatttat tttgttgtat gtttcacaag
     9121 aaatattaag aaatgcctta tttttttcta accaatcttc atcaaatata gggtatatgt
     9181 ttttcaaagt taatcacacc ccctcaaaaa aaaataataa aacaaaatat ttttaaaagt
     9241 tttgcaagac tgagaggcga attattatga taaatgcgta attctactat cacacttaca
     9301 aattgctatg ctttaattat aaataattgt attactttgt aaatatagtg tccatattta
     9361 tttatattag gaagtataat ttctcacata tactttaatt acagaagtac atgcagtgta
     9421 gatgacggtt ataaaatgta ggaaaatggc gaaatgaaaa tgctgctttt tttatataaa
     9481 cgccaagata aatatggaca agtttattct ttttatgaaa ggaaatgttt taatctataa
     9541 tactttccaa cgatagaggc gatcgagacg ggttctttgt caacgtcggt tggtgagaca
     9601 ataacggcac tgagacatta tggtttccta atctctgctt ctatttatag actgatatcg
     9661 attaataatg attgatcctt cgaaaataag gggtgaatgt gtataaaaaa tgatgccatt
     9721 gttttctgga tttcataatc ttgcactcag tttcgctgta ttgatatagg attacccaga
     9781 tggctgagac gcctgagggg gaacgtgcat actgttgaca gtcgcctctt tactttaatg
     9841 gtgggaggcg tttatcgcag tagcgggctg acttctcagt gttttcactt tcgagaagtc
     9901 taatcagcca tgcaaagcag tacaacgaaa tcttgttgca tatgagattt ctgtactgct
     9961 cctaaaatcc aatttggctt caatcaagcc gaattgagtt gaagggccgg ctctaggaaa
    10021 gcgaaaccat gatgacaatt aaaaccacaa atcgtaatgc ggacaagttt aataaaaata
    10081 ttacaacaat agcatcaaaa atttttaaga cccattccgt tttttatatg cacttacaag
    10141 tatcttagaa atgattaaga ttccagaaat ttctataact ataagacaca cgtttgtttt
    10201 attattaatc ctttcaatcg ggccatttgt taaatgagga tgatgcatag tatttgaggt
    10261 tcagggcaaa tatttgatga atgtttgcaa cgattaggta aaatgtcgat gatatcatgg
    10321 gttacactat cacagtaaat aaagctcatt ggtccttcga tatctttcgt tgatttgaat
    10381 tcgtcaaaag ctaagtgctc aggtaaagca tcatgtgcgt atgtctttac aaattactca
    10441 cctcttttaa atggcaatga acagttgaag gtgatacaca tagactttta gcgacatctt
    10501 tttcagatgt gaatttagtt agcttccgcg tcatcaagcg tttgacctta tttgagactt
    10561 cggggttgaa tatagggtgt tttagttata aaggtttgtt gacaagcttt aaagtaaaaa
    10621 cgatgcttct tcaaatttaa ataaacaggt ctttcaaaat taaaaaacgg gcagtaaatc
    10681 tctcaccaac cgttaaaaat acacaaccaa atatatacaa cctaaaaaac agggaaatgc
    10741 actgatttgt acacttccct atcataacat tttatttttg atttgccttt ggacctgtcg
    10801 gatatttata atctgctggg ttcaatttgt tgaaatcagg atttttatag aatcggaata
    10861 agtctccatt caagacttta tcactcattt ctaagtcatg ttgtacctgc tttttgtcct
    10921 tatcaagatc tgctggcggt gtttctaacg gttgattgtc tctgttactg taggctttac
    10981 cgttgacata tttataattt ctcgtcacga aatcaccatt acggaatggt acaatttctt
    11041 tatggtcctt ggataacata tctgtaccaa acatgagata gtttttcgta tcaataccta
    11101 acaagtgaag taatgttggc atcacatcaa cttgacctgc atacgtgtcg tccactttag
    11161 gctcaacccc tggtgctttt aaccagaaac ccgttgcatt caagtcatta aacttcgctg
    11221 gtgtaatctc ttcacctaat aactgtgaca tcgctttgtt atgattttct gagataccat
    11281 aatggtcacc gtaaatcata atgactgaat catcgtacag accacgctct ttcaagtcat
    11341 taatgaactg ttctaacgac tcatctaagt aacgcgctgt ttgaatgtaa ccatcgaccg
    11401 ttgagttacc tgtgtcacct tttgcaatcg ttgcgtcttc atcacttaat gtaaaaggat
    11461 aatggttcgt caacgtaatc aaatgtgaat agaacggttg tttttcttta gataagtaat
    11521 ccgccgcctc tttgaaaaac tctttatctt ttaatccaag gttttctaag ttttctggag
    11581 acatgtcaaa atatgttgca tcataaaact tatcgatacc aaaatgtctg tatacttggt
    11641 cacggttcca aaatgtttta tagtcaccgt gcatgacatt tgttgtatag ccttgttgtt
    11701 gatgcataat agctggtaaa gattgaaaag tattgtcacc ttttaatgaa aaggccgaac
    11761 cttgaggtaa accatacata ctgttgtcca ttgtgaactc agcgtctgac gttttacctt
    11821 gtccagtttg gtgatagaaa tgtgggtaat atctaaagtc ctcatt
//
