LOCUS       NZ_CP066713            11044 bp    DNA     circular CON 14-APR-2024
DEFINITION  Staphylococcus pseudintermedius strain DSP026 chromosome, complete
            genome.
ACCESSION   NZ_CP066713
VERSION     NZ_CP066713.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 2567628)
  AUTHORS   Ferrer,L., Perez,D., Fonticoba,R., Vines,J., Fabregas,N.,
            Madronero,S., Meroni,G., Martino,P., Martinez,S., Cusco,A.,
            Migura,L. and Francino,O.
  TITLE     Whole genome sequencing and de novo assembly of Staphylococcus
            pseudintermedius: a novel pangenome approach to unravel pathogenesis
            of canine pyoderma
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2567628)
  AUTHORS   Ferrer,L., Perez,D., Fonticoba,R., Vines,J., Fabregas,N.,
            Madronero,S., Cusco,A., Migura,L. and Francino,O.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2020) Servei Veterinari de Genetica Molecular,
            Universitat Autonoma de Barcelona, Edifici V, Travessera dels
            Turons, Bellaterra, Barcelona 08193, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUL-2020
            Assembly Method                   :: Flye v. 2.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 303x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016455085.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:32:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,471
            CDSs (total)                      :: 2,390
            Genes (coding)                    :: 2,301
            CDSs (with protein)               :: 2,301
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 63 of 89
            Pseudo Genes (incomplete)         :: 28 of 89
            Pseudo Genes (internal stop)      :: 9 of 89
            Pseudo Genes (multiple problems)  :: 10 of 89
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:05:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 879788
            Orig. end                         :: 890832
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP066713.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11044
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6044](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6044
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(321..335)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 23.65"
                     /tool="antismash"
     misc_feature    322..333
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.25"
                     /tool="antismash"
     misc_feature    817..826
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            1417..2349
                     /locus_tag="JGZ50_RS03950"
                     /old_locus_tag="JGZ50_03950"
     CDS             1417..2349
                     /EC_number="2.7.1.107"
                     /GO_function="GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166450.1"
                     /locus_tag="JGZ50_RS03950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_03950"
                     /product="diacylglycerol kinase"
                     /protein_id="WP_014613461.1"
                     /transl_table=11
                     /translation="MRKRARIIYNPTSGKEVFKRALPDVLIKMEQAGYETSAYATQKVG
                     DATEEAARAIEAQYDLLIVAGGDGTLNEVVNGIAEKPNRPKLGLIPMGTVNDFGRALHL
                     PTDIFEAVDVILDGKTVQVDIGKMNSRYFINLAGGGKITEVSYEAPSKLKSIVGPFAYY
                     IKGFEMLPQMHAVDVRIEFDSQVFEGEIMLFLLGLTNSMAGFEKLVPDAKLDDGMFTLL
                     IVEKANIAELGHIMTLASRGEHIKHPKVHYHKAQSVNISSFSDMPLNVDGEYGGQLPAN
                     FLNLVRHIEVFSPAQEDNALLIDEPTQSE"
     gene            2521..3885
                     /gene="rlmD"
                     /locus_tag="JGZ50_RS03955"
                     /old_locus_tag="JGZ50_03955"
     CDS             2521..3885
                     /EC_number="2.1.1.190"
                     /GO_function="GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rlmD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613462.1"
                     /locus_tag="JGZ50_RS03955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_03955"
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="WP_199274919.1"
                     /transl_table=11
                     /translation="MVLVKKNEVYEGKVVDLTHEGHGVIKQDRYPIFIPQTLIDEEVQY
                     KVIKVKKNFAIGKLMNVKTASPDRVTPPCDYYQQCGGCQLQHLSYRAQLEMKRKQVINL
                     FHHKGKMTEVPIHDTIGMDDPWRYRNKSQIPVGTNRNGQIQMGFYRQRSHDIIDMDHCL
                     IQNDDQNDMMNVIKSLLEKYHIPAYNEHIHQGLVRHVVIRKGYYTNEVMVIFVLNGKKL
                     PHHNDIVTALTSQFPQIVSIKLNFQTNKTNIIMGHTSKTIFGKDIIEDTLDDLTFEIGD
                     LSFYQINVTQTQKLYRQALQYANLTGNEVVLDAYCGIGTISLFMAPHAQHVYGVEVVPE
                     AIEDAKRNARYNQLNNTTFVAGAAEDVILKWQQDGIQPDVVTVDPPRKGCDATFIETLN
                     ALAPKRIVYVSCNPSTQLRDIEMLSTQYELKEMTPVDMFPHTTHVETVALLVRKY"
     misc_feature    4035..4053
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.21"
                     /tool="antismash"
     misc_feature    4115..4129
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 26.38"
                     /tool="antismash"
     misc_feature    complement(4140..4155)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 23.45"
                     /tool="antismash"
     gene            4200..4949
                     /locus_tag="JGZ50_RS03960"
                     /old_locus_tag="JGZ50_03960"
     CDS             4200..4949
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="JGZ50_RS03960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="JGZ50_03960"
                     /product="hypothetical protein"
                     /protein_id="WP_037542633.1"
                     /transl_table=11
                     /translation="MGGINCGLSNNRVVKNDMYRLEYALYTKYVFKCFENFCNANTWVE
                     EFTLLEIDDNDNALVAYKKALFAIQEAITNLQSAKNALINSYNEIAPLFKFSQLKNGSI
                     NQYKKYDHSFAKGVFEKAGVVTNETKVWESVIHSLQNGGEVDYLNDQQNKLIVFENTLQ
                     NLFEEYQKLEKYIRQGVSHVAIRDIEVDITPLTAMALTKISELMSSLTYLCLVEYSAHT
                     SITGKTIDVLDLLPKTNKNSIQHLKAN"
     gene            5001..6044
                     /locus_tag="JGZ50_RS03965"
                     /old_locus_tag="JGZ50_03965"
     CDS             5001..6044
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="JGZ50_RS03965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_03965"
                     /product="YcaO-like family protein"
                     /protein_id="WP_014613464.1"
                     /sec_met_domain="YcaO (E-value: 2.5e-22, bitscore: 77.2,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLENVSEHGVSGFYTYHFTEGNSFRPCATRIIEKEAIKATKDEFM
                     ELMLISSKSFPFVFFGGKSLGNFKLPIHLHPFMWQKRTIQDVNWYNEKNVEYIPFYNYK
                     DGSLKYIPKELSVYYEKAKGIGLSFSPQEGYSYDRDIVLAKVKAVMELIEKDIVTLWWH
                     RETSSRIIVIDDDTDENIKIIRNQLLNQGIDITLLDIENDLGVYVVVCILKQDDFPSIT
                     YGSAAHFEIKGAVKHAIFEAISCIVGLRYEFIHFKKIRTSFTYPDFISDSPLTNLSDYK
                     EILNLKTAINKYDIYYSYISADEGYLVKAYCYELQPTLYSATVPLTKRFFRVNKKEVRI
                     KEDFPFI"
     gene            6055..7245
                     /locus_tag="JGZ50_RS03970"
                     /old_locus_tag="JGZ50_03970"
     CDS             6055..7245
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="JGZ50_RS03970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_03970"
                     /product="MFS transporter"
                     /protein_id="WP_014613465.1"
                     /transl_table=11
                     /translation="MKTKRIIASDFLTTMMSSALTIYIYWFAYQYFSSQLIISIIGFGQ
                     LSGVFLSSLGGGISDSMNKLFFIKTLNFFKVGLLFMVLILENVVNMEILLPLFMFASTV
                     IGGLQSPTLESLVPFLASSDEALFRINAVVSSSTQVASIMAIVLSAVYISFLSFVTIVF
                     VTLFITAIATILLVGLTVETPLKSTSVVNNMKHGASYIFKTPYIRNLIPVALVMNFSFW
                     SIFLLLPKIANDSFSFLKTSYSGMELSFALGAGLGGILFAKFFYDVKDKYRLFKRSLFS
                     QSYVLLLLGIVLLIPNSVLAYTGMLLCWFLYALINTIFSILYFGKIQLNVPREMIGSVF
                     GAILTLFSLVNPIAAIMSGVLVSHFKIPLLIILLSSLMLLAAWSIRFLADVETVFD"
     gene            7259..7981
                     /locus_tag="JGZ50_RS03975"
                     /old_locus_tag="JGZ50_03975"
     CDS             7259..7981
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="JGZ50_RS03975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_03975"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_199274920.1"
                     /transl_table=11
                     /translation="MSILTLKNIRCEMDGKLLFEADGLTIEEGDVIGLIGKNGAGKTCL
                     LKILAGELSDYAGYISGNRLTYFSELCITEDMTQSGGELSISSFLNALRQSVPLYLLDE
                     PTTYLDQHHLKKVVRTIRRSPSSFCIASHDRAFLDAVATKIWLLEQGKITEYNGSFSDY
                     VLARSMQLSQYEQELKQYQKEKKKIKQSLRMMKEKQDQKKGKPKKMSTSEYRIAGIKTK
                     HGVKQKKLQKRITRQQKS"
     gene            8107..8625
                     /locus_tag="JGZ50_RS03980"
                     /old_locus_tag="JGZ50_03980"
     CDS             8107..8625
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="JGZ50_RS03980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_03980"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_199274921.1"
                     /transl_table=11
                     /translation="MWHTPTIHLNSGDKLGIIGRNGAGKTTYLNYLISVLPSTYKICYF
                     RQNHFQYLDPKMTVYEAIVEATNGYLSEHHIRTLLALLDFKRDKVFRLIGDLSRGEHVK
                     ISLLSLLVSESDILILDEMTNFLDIKAIEAVEKVLATYSGILIFVSHDQYFVQRLATSI
                     LDIDTYKIM"
     misc_feature    8817..8831
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 24.36"
                     /tool="antismash"
     misc_feature    complement(9005..9019)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.13"
                     /tool="antismash"
     gene            <9158..9388
                     /locus_tag="JGZ50_RS03985"
                     /old_locus_tag="JGZ50_03985"
                     /pseudo=""
     CDS             <9158..9388
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613462.1"
                     /locus_tag="JGZ50_RS03985"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_03985"
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /pseudo=""
                     /transl_table=11
                     /translation="DVVTVDPPRKGCDATFIETLNALAPKRIVYVSCNPSTQLRDIEML
                     SAQYELKEMTPVDMFPHTTHVETVALLVRKY"
     gene            9862..10518
                     /locus_tag="JGZ50_RS03990"
                     /old_locus_tag="JGZ50_03990"
     CDS             9862..10518
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR01716"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /locus_tag="JGZ50_RS03990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JGZ50_03990"
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="WP_199274922.1"
                     /sec_met_domain="TIGR01716 (E-value: 4.1e-26, bitscore:
                     89.1, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNYGKTFRKIRKSRGIKLKDIAKMGISVSQLSRFENGETNLTIS
                     KFILILNEIMMPLDEFMNEAHDFRNNRLNERFEHMIYFTIENDILRMKQLLFDEMKKNT
                     QRSHFYYLNLILIKIRLQVMTGELYYTKDDLEVLIDYLFTTEYWGYYELYLFTNTLDVI
                     NHETMMLLSREIVSQTKALNKLQLYRRMLTAMLLNGYMTCIQRQKMKDAIFLKNV"
     misc_feature    complement(10711..10726)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 26.19"
                     /tool="antismash"
ORIGIN
        1 tcaagaagaa gaattgttaa atggtggcga aattttacaa gaaacacgtc gttttgatga
       61 atccacaggt aaaacgattt taatgcgtgt taaggaagcg tcagatgact accgttactt
      121 ccctgaacct gacatcgtac ctttatatat cgatgaagca tggaaagcgc gtgttcgtga
      181 atcgattcct gaattaccag atgcacgtaa agaaaaatac gtaaaagaat tcggtttacc
      241 tgcatatgat gcacatgttt taacattaac aaaagagatg tcagatttct ttgaagcggc
      301 tgttgaagaa ggcgcagatg tgaaaatgat ttcgaactgg ttaatgggtg gcgtaaacga
      361 atatctcaat aaaaatcaaa ttgagttaca agatacaggc cttacaccag caaacttagc
      421 aggtatgatt aaattaattg aagatggcac aatgagtagt aaaattgcga aaaaagtgtt
      481 cccagaatta gcgcaaaatg gtggggacgc aaagcaaatt atgaaagata aaggtcttgt
      541 tcaaatttct gatgaaggtg ctgtgttaga atttgtacaa caagcaattg ctgaaaatcc
      601 acaatctgtt gaagattata agaatgggaa aggtaaggcg atgggcttct tagttggaca
      661 aattatgaaa ttgtcgaaag gtcaagcgaa tccacagctt gcaaatcagt tgttgaagca
      721 agagttagat aaacaatagg ttgttttgaa tagaggcgag tagatggaat gcaatggtga
      781 tgattgtatt tcacactcgt cttttttgtg acattttgtt ttttgagtat gacgtaagat
      841 gtcgtattga ttataacacc ttgcaatcgc agaactaggg ctcgctttca taatgtgaat
      901 atctgtatgg attgcctttt aggatttgct ttcctagggc tgttcccaag taatcaacgc
      961 ttaactgaaa ctgttataag gtggaggcgt tgatggattt ccggcgcatc ttttccctca
     1021 ggagtcgcgc cctgaatggt aatcagttgc gtaacaagga atgaaagcga gcttgttcta
     1081 gcaatcttag ttgcggtgct caaacgagtt gaaatgacga ataactctta ccaaacagac
     1141 ggcgcatgta ttaatcatac gcatgcattt tttcactcat tttatttata tgattattaa
     1201 ttgatcttag gtgtagtggt gaaagaatga tgaagtaact caatttcttt gcttggctag
     1261 gaaagttgaa atgtcaacat atgacgcaat cgccttttga aaacgggatt gacgttattt
     1321 tcttatcatt tttacgcttt tggttgtttt tagctttcat tacatctttg tttactataa
     1381 tataagttgt gagtactgaa aaattgaggg atataaatga gaaaacgtgc gagaataatc
     1441 tataatccta catcgggtaa ggaagtgttt aaacgtgcat tacctgatgt actaattaaa
     1501 atggaacaag ctggttatga gacgagtgcc tatgcgactc aaaaggttgg cgatgcgaca
     1561 gaagaggcgg cgcgtgcgat tgaggcccaa tacgatttgt taattgtcgc tggtggagac
     1621 ggtacattga atgaagtcgt gaatggtatt gctgaaaaac cgaatcgtcc taagttaggt
     1681 ttaattccaa tggggacggt gaatgatttc ggtcgagcac tacatctccc aactgatatt
     1741 tttgaagccg tggatgtcat tttagatggt aaaactgttc aagttgatat tggtaaaatg
     1801 aatagccgtt attttattaa tttagctggc ggtggtaaaa ttacggaagt ttcgtatgaa
     1861 gcaccgagta aattaaaatc gatagtcgga ccttttgcgt actatattaa aggatttgaa
     1921 atgttacctc aaatgcatgc agtcgatgtg cgtattgagt ttgattcgca agtctttgaa
     1981 ggtgaaatca tgttgttctt actcggctta accaattcaa tggctggttt tgagaagtta
     2041 gtaccagatg cgaagttgga tgacggtatg tttacactgt tgattgttga aaaggcaaat
     2101 attgcagaat tgggacatat tatgacactt gcctcacgcg gtgaacatat taaacatccg
     2161 aaagtacatt accataaagc gcaatcggta aatatttctt cctttagcga tatgccgtta
     2221 aacgtagatg gtgaatacgg gggtcaatta ccagcgaact ttttaaattt agttcgtcat
     2281 attgaagttt tttctccggc gcaagaagat aatgcgttgt taattgatga gcctacgcaa
     2341 tctgagtaac gatccagagg tgtgctgaaa ataaataggt agtgtacgca aaaccgcatg
     2401 atgtaatgac attactctcg tgggagcggg acgatgaaat ttgactcaga tgttaagttt
     2461 ttggtcctgc tcctttccct ttgaagtaga ttctgtttat aaaaaagtga gatgaaattg
     2521 atggttttag taaagaaaaa tgaagtgtat gagggaaaag tggttgattt gacacatgaa
     2581 gggcatggtg tgattaaaca agatcgctat ccgatattta tccctcaaac gttaattgat
     2641 gaagaagtgc aatataaagt gattaaagtg aaaaagaatt tcgcgattgg caagctgatg
     2701 aatgtgaaaa cagcaagtcc agatcgtgtt acaccgccat gtgattatta ccaacagtgt
     2761 gggggatgcc aacttcaaca tttgtcgtat cgtgcgcaat tagagatgaa gcgtaaacaa
     2821 gtgattaatt tgtttcatca taaaggaaaa atgacagagg tgccgattca tgacacgatt
     2881 ggaatggatg atccttggcg ttatcgaaac aagtctcaaa ttccggttgg gacgaatcgc
     2941 aatggtcaga tccaaatggg tttttatcgt caacgcagcc atgatattat cgatatggac
     3001 cattgtctca tacaaaatga tgatcaaaac gacatgatga atgtgattaa aagtttactg
     3061 gaaaaatatc acattccagc ttataacgaa cacatacatc aaggtctcgt acgtcatgtc
     3121 gtgattcgta aaggctatta tacgaatgaa gtgatggtta tttttgtgct caacggtaaa
     3181 aagttaccac atcataacga tattgtgacc gctttaacat cacagtttcc gcaaattgtg
     3241 agtatcaagt tgaatttcca aacgaacaaa acgaatatca ttatggggca tacgtccaaa
     3301 acgattttcg gtaaagacat tattgaggat acattagatg atttgacttt cgaaattggc
     3361 gatttgtcct tttatcaaat caatgtgaca caaacgcaaa agttgtatcg tcaagcgtta
     3421 cagtatgcga atttaacggg taatgaagtc gtgttagatg cgtattgtgg tattgggacg
     3481 atcagtttat ttatggcacc ccacgcccaa catgtatatg gtgtagaagt tgtccctgaa
     3541 gcgatagaag atgcgaaaag aaatgcgcgc tacaatcaat tgaacaatac aacttttgtc
     3601 gctggtgctg ctgaagacgt gattttaaaa tggcaacaag acgggattca gcctgatgtg
     3661 gtgacagttg acccaccgcg caaagggtgt gacgcgacat ttattgaaac tttaaacgcg
     3721 ttagccccaa aacgtatcgt ctatgtgtca tgcaatccaa gtacacaatt acgagatatt
     3781 gaaatgctaa gcacgcaata tgaactaaaa gaaatgacac ctgtcgatat gtttccgcat
     3841 acgacacatg tcgagacagt tgcgttatta gtaagaaaat attgatatag attaatcact
     3901 caagggtttt ttgagaacat ttttgagaat gtgacttaaa accctttttt gttttggtgc
     3961 tattttagga gcgttgatac tggaactgat ttgacaaata ggtttgtcgt taatagacat
     4021 tagctttttt aaagtattgt ttagaaacac agttgtgaaa ttgtaaaaat attgagatta
     4081 gacattttgt gtttattaat atctttctag aattaatatt cagaaaattt ggaaaaatag
     4141 tttacatttg tgtatattta tgctacattc atgatggaat attaagagga ggtgccatta
     4201 tgggtggtat taattgtgga ctttccaata atagagtcgt aaagaatgat atgtatcgat
     4261 tagaatatgc tttatacaca aaatatgtgt ttaaatgttt tgaaaatttt tgtaatgcaa
     4321 atacatgggt tgaagagttt acacttttgg aaattgatga taatgataac gcactagttg
     4381 cttataaaaa agcattattt gccattcaag aggcgattac taatttacag agtgctaaaa
     4441 atgcactaat caatagttac aatgagattg cgcctttatt taaatttagt caattaaaaa
     4501 atggttcaat aaaccaatat aaaaagtatg accattcctt tgctaaaggg gtatttgaaa
     4561 aggcaggagt tgtgacgaac gagacaaagg tttgggagtc agttattcat tctctgcaaa
     4621 atggaggaga agtagattat ttaaatgatc aacaaaataa gttgattgtt tttgaaaata
     4681 ctttgcaaaa tctatttgag gaatatcaaa aattagaaaa gtatatacga caaggtgtat
     4741 cacatgttgc aataagagat attgaagttg atattacgcc gttaactgca atggccttga
     4801 ctaaaatcag tgaattgatg agttcattaa catacttatg tttagttgaa tattcagccc
     4861 atacaagtat cactggcaaa acgattgatg tattggattt actaccaaaa acaaacaaaa
     4921 atagtataca gcatctaaaa gcaaattgat ttttatatac tgtgtctata cggtacagtt
     4981 ttttatgtag ggggattaat atgttagaga atgtttctga acatggggtt tcaggatttt
     5041 acacatatca ctttacagag ggaaactcat ttagaccatg tgctacaaga attattgaaa
     5101 aggaagctat aaaagctact aaagatgagt ttatggagtt aatgcttatt tcaagtaaat
     5161 ctttcccgtt tgtatttttc ggagggaaat ccttaggtaa ttttaaacta cccattcatc
     5221 ttcatccgtt tatgtggcaa aaacgaacta tccaagatgt gaattggtat aatgaaaaga
     5281 atgttgagta tatccctttt tataactaca aagacggatc tttgaagtat ataccgaaag
     5341 aattaagcgt ctattatgaa aaagcgaaag ggattgggct atcttttagt cctcaagagg
     5401 gttattccta tgatcgagat attgtacttg ctaaagtcaa ggcggtcatg gaattaatag
     5461 aaaaagatat tgttactttg tggtggcata gagaaacatc aagtagaatt attgtgattg
     5521 atgatgatac tgatgagaat ataaaaatta ttagaaatca attattaaat caaggtatag
     5581 atatcacgtt attagatatt gaaaatgatt taggtgttta tgtggttgtg tgtatcctca
     5641 agcaagatga ctttccatca atcacttatg gtagtgcagc gcattttgaa attaaaggtg
     5701 cagttaaaca tgcaattttt gaagcgattt catgtattgt gggtttgagg tatgagttca
     5761 ttcattttaa aaaaatccgt actagtttta cgtatcctga ttttattagt gatagtccat
     5821 taactaattt atccgattat aaagagatat tgaacttaaa aacagccatt aataaatatg
     5881 atatatatta ttcttatatt tctgctgatg aaggatactt agtgaaagcg tactgttatg
     5941 aattacaacc caccttgtat agtgcaacgg ttcctttaac taaacgtttc tttagagtaa
     6001 acaagaagga agtcaggatt aaagaagatt ttccgttcat atagaaggtg agttatgaaa
     6061 actaaaagaa ttattgcgtc agatttttta acaacgatga tgtcttcagc cttaacgatt
     6121 tatatatatt ggtttgctta tcaatacttc tctagtcaac tcatcatatc aatcatcggt
     6181 tttgggcaat taagtggtgt gtttctctca agtcttggtg gtggcatctc agacagtatg
     6241 aacaagctgt ttttcatcaa aactctgaac ttctttaaag tcggattact ttttatggtg
     6301 ttgatattag agaacgttgt gaacatggaa attttgttgc cactttttat gtttgcttca
     6361 acagtgatag gaggtttaca gtcacctacg ttagaatcat tggttccatt tttagccagt
     6421 agcgatgagg cgctttttcg tattaacgct gtggtttcga gttcaacaca ggttgcatct
     6481 attatggcga ttgttttatc tgcagtctat atttcatttc tgtcttttgt aacgattgtt
     6541 tttgtgaccc tttttatcac tgccatcgca acgattttac ttgtaggact gacggtggaa
     6601 acgccattga agtctacttc agttgttaat aatatgaagc atggggcgag ttatatcttt
     6661 aaaacgcctt atattcgtaa ccttattcct gtcgcattgg tcatgaattt ctcgttttgg
     6721 agtattttct tgctcttacc taaaatcgct aatgatagtt tttcattttt aaaaacctct
     6781 tatagtggca tggagctctc ctttgcttta ggagcgggct taggcgggat cttatttgcg
     6841 aaattcttct atgatgttaa agataaatat cgtttattta agaggagttt gttttcacag
     6901 agttatgttt tgttgttatt aggaatcgtt ttacttattc ctaacagtgt tcttgcctat
     6961 accgggatgc tgttgtgctg gtttttgtat gctttgatca acacgatttt ctctattctc
     7021 tattttggaa agattcaatt gaatgtccct agagaaatga ttggcagtgt ttttggagca
     7081 attttaacgc tcttttcatt agtcaatccc atcgcagcca tcatgtcggg agttctcgtt
     7141 tcgcatttta aaatacctct gttaattata ttgttgtctt cactcatgtt gttagctgcc
     7201 tggagtattc gtttcctagc agatgttgaa acggtatttg attaaagggg ggagggatat
     7261 gtctatatta acacttaaaa acatcaggtg tgagatggat gggaaattat tatttgaagc
     7321 ggatggttta acgattgagg aaggcgatgt cattggcctt atcggaaaga acggtgctgg
     7381 aaagacgtgt ttgttaaaaa tattagcagg agagttaagc gattatgctg gctatatatc
     7441 gggcaatcgt ctgacttatt ttagtgaact ctgtataacg gaggatatga ctcaaagtgg
     7501 tggagaacta agcatttcaa gttttctcaa cgcattaaga cagtcggtgc ccctctattt
     7561 gttggacgaa cctacaacgt atttagatca acatcatttg aagaaagtag taagaacgat
     7621 tagacgctcc ccatcttctt tttgtatagc gagccatgat cgggcttttt tagatgcagt
     7681 tgcgactaaa atatggttgt tagagcaagg aaaaataaca gaatataatg gttctttttc
     7741 agattatgtg ttagcacgtt caatgcagtt atcacaatat gagcaagagt tgaaacaata
     7801 tcagaaggaa aaaaagaaaa taaaacaatc acttcgaatg atgaaagaaa agcaagatca
     7861 gaaaaaggga aaaccaaaga agatgagtac ttcagaatat cgtattgcgg gtatcaaaac
     7921 taagcacggt gtgaaacaaa aaaagttaca gaagcgtatt acacgacaac aaaaaagtta
     7981 gaacagttaa aacaaccata tcaagttgag gacaaatatg atatttcatt tttatcacaa
     8041 cttcattgtt tacccaaaag aaaaattatc attcctagtc acgaagcgaa aatcgggcaa
     8101 aaaagcttgt ggcatacccc aactatccat ttaaatagtg gtgataaatt aggaattatt
     8161 ggaagaaatg gtgcaggcaa aacaacttat ttgaattact taatttcagt tttaccttct
     8221 acatataaaa tttgttattt tcgccaaaat cattttcagt acttagaccc aaagatgaca
     8281 gtatatgaag cgattgtcga agctacgaat gggtatttaa gcgaacatca cattagaact
     8341 ttgttggcgt tattagattt taaaagagac aaggtatttc gattaatagg agatttaagc
     8401 cgaggggagc acgtgaagat ttcattatta tctctcttag tttctgagtc tgatattttg
     8461 attttagatg aaatgaccaa ctttttagat ataaaggcta tcgaagcagt ggaaaaggtg
     8521 cttgctacat attcaggcat actgatattt gtgagtcatg atcagtattt tgtacaacgt
     8581 ttagctactt cgattctaga tattgatacc tataagataa tgtaggatgg acaattacaa
     8641 atattttgga gtgaaagaat ctccctccat caagcatcta tcaaatagtg atagtataaa
     8701 aaggacgtcc gtttgattag gtaccctgtt ttgatgacag tttaacaaaa acaataaaag
     8761 ttttaaggta cagactacta gctgtaatgc tatattattg acgcagagat gtgatgttct
     8821 catttttaaa agttgtgtag cctccgtgat caagttaaag gtgagtgttc aatatccatt
     8881 attaaagaaa gttattttat ttttggacgg taaaccaatg tcatcaaaaa taaaaatgaa
     8941 accatgcaat gataattcgt aatttctgtg tgaaacgtag gtaacatatg aaaaatcacg
     9001 ctataaattt cagaataata agaaattaca aaggggttac gtttaaggaa atttctaaaa
     9061 gagggatatc atcattccaa ccctcacgtt ttgataaagg ggagactcac gactgcaaga
     9121 atgctacagt agttgatttt tcgaatcagc gtagaatgat gtggtcacag ttgacccacc
     9181 gcgcaaaggg tgtgacgcga catttattga aactttaaac gcgttagccc caaaacgtat
     9241 cgtctatgtg tcatgcaatc caagtacaca attacgagat attgaaatgc taagcgcgca
     9301 atatgaacta aaagaaatga cacctgtcga tatgtttccg catacgacac atgtcgagac
     9361 agttgcgtta ttagtaagaa aatattgata tagatgaatc actcaagggt tttttgagaa
     9421 catttttgag aatgtgactt agaacccttt tttgttttgg tgctatttta ggagcgttga
     9481 tactggaact gatttgacag tgttaatctc tagagaatgg cgagtaatga agttttgaag
     9541 ttattctaaa tataaaataa aatcattgtt ctgaatattc aaaatacaca tttacatatt
     9601 ctgtattcat acgctatatt aaagatataa gattaagaag ttggtaaaat agcttattta
     9661 aattatttag ttttacgtgc tatctataaa atatgttcag ctcagcaaga atatcctcca
     9721 ttttgggttg acatcataaa ctaaaattaa tggcatatta aaaaagtatt tttttacgga
     9781 ggtaaaagca atgtaaaatt taatcatctt gatgcaacat agacatttat tcgtaatttc
     9841 tatatgaatt ggaggtatca tatgaaaaat tacggcaaaa cttttagaaa aataagaaaa
     9901 tcgagaggca taaagcttaa agatattgca aagatgggga tatcagtctc gcagttatct
     9961 cgctttgaaa atggagagac aaacttaacc atttcgaaat ttatattaat attaaatgaa
    10021 attatgatgc ccttagatga atttatgaat gaagcacatg atttccgaaa taatagatta
    10081 aatgaacgat ttgaacatat gatttatttt actattgaga atgacatttt acgtatgaaa
    10141 cagctattat ttgatgaaat gaagaaaaat actcagagaa gtcattttta ttatttaaat
    10201 ttgatattga ttaagattcg tctacaagtt atgacagggg aattgtatta tacaaaagat
    10261 gatttagagg ttttaattga ttaccttttt actaccgaat actggggata ttatgagctg
    10321 tatcttttta caaatacttt agatgttata aatcatgaga cgatgatgct cttatcacga
    10381 gaaatagtat cacaaacaaa ggcgcttaat aaattacaat tgtatcgtcg aatgcttaca
    10441 gcaatgttgt taaatggata tatgacttgc attcaacgac aaaaaatgaa agatgctatt
    10501 tttttgaaaa atgtatagag aggtgtttgt ttactgaaac agaaatttac gaaaagcttg
    10561 tatttcaata tgctaagcat tttttaattt atagtaaatc ctctagtgag gacgctatca
    10621 ataaaatgaa aaaaatcatt gaagttatgc gcttagtaga aagtcatcat attgcatctt
    10681 actatgagaa tcatttaaac catatattat gtgaaaaaaa tgtgcatata tgggacagtt
    10741 tgtaaaaaac attaaattgt atattacaat gtaaatgtag gcttacaata taattagtaa
    10801 tatcagaggt ggataatatg attattgagt ttacaagaga agaattgtta cagatgcaaa
    10861 ttattgatgc aataatgaaa tcctctaata gcgttttaaa tgaaatgaat acaagtaagt
    10921 atattacgcg gcgtcgatat tagtaaaaag gtgatggatt atggtttatg agttagaaga
    10981 aggtgtacta cgaaaaatag aggggagtag taatcctgta ttgattccac ttacaacgat
    11041 ttat
//
