LOCUS       NZ_CP065920            11614 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Staphylococcus pseudintermedius strain SP_11306-1A chromosome,
            complete genome.
ACCESSION   NZ_CP065920
VERSION     NZ_CP065920.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 2605463)
  AUTHORS   Byukusenge,M., Banovich,F., Li,L., Kuchipudi,S.V., Jayarao,B.M. and
            Naikare,H.K.
  TITLE     Complete genome sequences of Staphylococcus pseudintermedius strains
            isolated from canine superficial pyoderma lesions in Georgia, USA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2605463)
  AUTHORS   Byukusenge,M., Banovich,F., Li,L., Kuchipudi,S.V., Jayarao,B.M. and
            Naikare,H.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2020) Veterinary and Biomedical Sciences,
            Pennsylvania State University, Wiley Ln, University Park, PA 16802,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 233x
            Sequencing Technology             :: Illumina & Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016126755.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 13:13:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,469
            CDSs (total)                      :: 2,387
            Genes (coding)                    :: 2,345
            CDSs (with protein)               :: 2,345
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 42
            CDSs (without protein)            :: 42
            Pseudo Genes (ambiguous residues) :: 0 of 42
            Pseudo Genes (frameshifted)       :: 13 of 42
            Pseudo Genes (incomplete)         :: 29 of 42
            Pseudo Genes (internal stop)      :: 14 of 42
            Pseudo Genes (multiple problems)  :: 12 of 42
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:44:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2098572
            Orig. end                         :: 2110186
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065920.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11614
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11614
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11614
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6614](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6614)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(149..1930)
                     /gene="recQ"
                     /locus_tag="JC281_RS09890"
                     /old_locus_tag="JC281_09890"
     CDS             complement(149..1930)
                     /EC_number="3.6.4.12"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recQ"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424488.1"
                     /locus_tag="JC281_RS09890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JC281_09890"
                     /product="DNA helicase RecQ"
                     /protein_id="WP_143951510.1"
                     /transl_table=11
                     /translation="MEQTLSHYFGYKSFRPGQKEIIERVLNHHHTLGVLPTGGGKSLCY
                     QVPGLMFKGTTIVISPLISLMKDQVDQLTAMGISAAYLNSSLSTQAQKEIETQLVNGEL
                     KFLYIAPERFEQTYFEMLLRRVDISLVAFDEAHCISKWGHDFRPSYQAVIQRVLTMPQQ
                     FSIVALTATATTEVQQDIMERLMISRQHLVKTSIRRRNLKFQVNGTYQRQKFVLDYIQK
                     HSQKAGIVYCSTRKQVEALYEALEDADIPVSYYHAGMTAKQRDKAQNDFLYDRTRVVVA
                     TNAFGMGIDKSNVRYVIHYNMPGDLESYYQEAGRAGRDGLDSDCILLYSDRDIALHQYF
                     IGASKADDDYKERMGEKLTKMIQYTKTTKCLEATLVHYFEPNEKLEECHQCSNCIDKNK
                     TYNMTKEAKMIISGVARLRQQEGYQTVIQVLRGEVSDYIRHQGYENLSTYGLMKDYTTG
                     ELHHLIDELRFKGFLNEHDEILMCDASVEKLLSEDTQVFTRPFKRKSKETVHINTVEGV
                     DRTLFEKLVEVRREMSERLNMPPTNIFTDYTLEAFAKRKPVTKQEMIQIDGVGSYKLKH
                     YCPEFLEAIQEYKMHSS"
     gene            complement(1940..3817)
                     /locus_tag="JC281_RS09895"
                     /old_locus_tag="JC281_09895"
     CDS             complement(1940..3817)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166146.1"
                     /locus_tag="JC281_RS09895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JC281_09895"
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="WP_015728743.1"
                     /transl_table=11
                     /translation="MEAYKIERLNKSYADKVIFDDLQLSISNQERIGLVGINGTGKSSL
                     LKVIAGMDEDFDAVQSHPKQYRIRYAAQNHNLEPTLTVYEAVYSADSKELQVIKRYEEA
                     TAQYAEQQTDKAFDAMMAAQADMDAYSAWDYSAEIKTILSKLGIHDTTRRMSELSGGQQ
                     KRVVLARTLIEKPDLLLLDEPTNHLDFESIHWLIQFVKSYPKTVVFVTHDRYFLNEVAT
                     RIVELRNGKLDTYPGNYEAYIAMRAEREEIEVRQQTKQRALFKQELAWMRAGVKARTTK
                     QQARQDRFHDLKEQVQSHQVQGKGSLNLAYSRLGKQVFELENVTKRIDGRTLFENVSTL
                     IQKGVQIGIVGPNGVGKTTMLNILAGEDQHFEGVLKIGQTVKVAYFKQTDERLNRDVRV
                     IDFLREESELARQKDGTTVSITQLLERFLFPSVTHGKKVYKLSGGEQKRLYLLKLLVHQ
                     PNVLLLDEPTNDLDTETLTILEDYIQTFGGTVITVSHDRYFLDKVVDCYWYIHDGQIDM
                     ILGQFEDYLAYQKQLEQAATQVASVKQQPTLQPKKKKGLSYNEKREYDMLLVRIEETES
                     RLAEIDAEMIEANADYAKIKALNEEKETLEAQYDTDMTRWSELEEILEQ"
     gene            3929..4744
                     /locus_tag="JC281_RS09900"
                     /old_locus_tag="JC281_09900"
     CDS             3929..4744
                     /GO_function="GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019168536.1"
                     /locus_tag="JC281_RS09900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JC281_09900"
                     /product="ZIP family metal transporter"
                     /protein_id="WP_015728742.1"
                     /transl_table=11
                     /translation="MLDYISNMPPFLQALLAGVITWLLTALGAATVFVFKSVNEKVLNS
                     MQGFAAGIMIAASFWSLLQPAIESSSGSTVPWLPAAIGFLLGGVFIRSLDYVIPHMHRN
                     ATDESQHQEGVETSLNKNTLLLLAITLHNIPEGLAIGVAFGGIATGNEHATLLGALGLA
                     IGIGIQNIPEGAALSLPIHASGQSKWKAFNYGQASALVEPVFATVGAAAVLVVTPILPY
                     ALAFAAGAMIFVVVEELIPDSQAGKNTDLATLSLMVGFTIMMILDVALG"
     gene            complement(5001..6614)
                     /locus_tag="JC281_RS09905"
                     /old_locus_tag="JC281_09905"
     CDS             complement(5001..6614)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /locus_tag="JC281_RS09905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JC281_09905"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_272914040.1"
                     /sec_met_domain="TIGR01193 (E-value: 1.3e-203, bitscore:
                     675.4, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINILGSYFMKSIIDFYIPQKLKTWVSMISMALIASYIMQQILSY
                     LQKYFLILLVKTYSINILSPYIKHLFRLPITFFETGRAGDILSRLNDANSITDALASTM
                     LSLFLDVTIIVGISIFLYYQSTILFVLILIIIPIYAVIVFGFMNSFERKRIKSMEANAT
                     LSSYLMENITGMETIKALTCEKKCYKQIKYNFSNYCEKTFLLNKLNIKQNVLKKVVQLI
                     LNVSILWVGANLVIDNQISIGTLLVFNSLMIYFMNPIENIMNLQLKLQIAGVANNRLNE
                     IFTVKSEFEKNKLDIKLNQNSSKLIFSNISYGYVYEKEILTDINITIEHNSKVAFIGES
                     GSGKTTLAKLMVSFFEPSKGKITLGGLDITNINKSVLRQQIIYIPQQTYIFNGTILENL
                     LLGVVKEVTQKDIDSAIKMVELKSFIDSLPLGLDSVLSSDFSALSGGQRQKIALARALL
                     TNSSILIMDEATSNIDILTERKIINNLMALNKTIIFISHNLNITQKVDKIFVLAKGKII
                     EEGSHEFLLKAKGYYSNLIN"
     gene            complement(6571..7146)
                     /locus_tag="JC281_RS12560"
                     /old_locus_tag="JC281_09910"
     CDS             complement(6571..7146)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /locus_tag="JC281_RS12560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JC281_09910"
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_187530240.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 9.9e-41, bitscore:
                     135.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFSKRFYRAQVDSQDCGAAALAMILEFYGSHYSLDFLRRKLRTT
                     VNGTTAYGLVQVADKLGFETVPIKADISLFEIDDLQYPFIVHVKKNNNHFHYYVVIRCD
                     KKYIYIADPVVNLTKMPYREFKEEWTGVTILLKPTSLYKPIKQDKVRLTYFIPLLLKEK
                     KIDYKNNNHFHFSQYDKYFRFIFYEVNY"
     misc_feature    7325..7338
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: strong, score: 21.85"
                     /tool="antismash"
     misc_feature    7352..7367
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 24.05"
                     /tool="antismash"
     gene            complement(7521..7829)
                     /locus_tag="JC281_RS09915"
                     /old_locus_tag="JC281_09915"
     CDS             complement(7521..7829)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024424.4"
                     /locus_tag="JC281_RS09915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JC281_09915"
                     /product="DUF3884 family protein"
                     /protein_id="WP_063284913.1"
                     /transl_table=11
                     /translation="MIKISLKKILCQLSQKKLYEKTFQSIYIVDFSLLDRAPLFKDEFK
                     VIGTWYSYSGKRWICHTELSTEQFKKMITKNIDHKDLEKVKFYLDYLPFSITNEIPF"
     gene            complement(7904..9157)
                     /locus_tag="JC281_RS09920"
                     /old_locus_tag="JC281_09920"
     CDS             complement(7904..9157)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /locus_tag="JC281_RS09920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="JC281_09920"
                     /product="glycosyltransferase"
                     /protein_id="WP_143951511.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 5.7e-19,
                     bitscore: 65.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRKNKAFLNISCETYNKIKELNKFIESKDWESKRVKNDTELIEK
                     INKLEIISEYTTISVVYIVKNERDYIIKSLLSILDLADEIIIVDTGSEDNTLDLIKKMC
                     DKKIKIFTFNWCDDFSKARNFANAKATCDWIMILDADEIVRKNDNLKFYLTYLRIFDDF
                     ENTAFNFKVIRDEEVYKTSKLIRNTNTLTYKGRVHETFFSLNNSVSYANLNVEVLGIRR
                     GSQKKTNYYNKLLLKTIIDFPKEGRWYYLYLRDNIEDINYNQMLKFCKKIIFKRDAISG
                     IEINNNYYSVRIIFLLMFKLLEESNHERFTYYMELLEKDFNHTQDFIFLKYSYYIKKLN
                     IDATNLLKSFLENYDLLNEGDVIFSKECTHSLLGASLFWGGYLKESKKVFEELEINSKD
                     TVCFYNKKFIYDCLSTLS"
     misc_feature    10189..10202
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.13"
                     /tool="antismash"
ORIGIN
        1 attcattgta aaaataacac aatttgcatc taaaaaattt aaatcatcta ctatcgacac
       61 gtctcacgtt tccattcttt accactcaat aagaaatcgt ttatttgtaa aaaaggagcc
      121 aggaattccc ccagctccat ctgatgtttt acgaactatg cattttatat tcttgaatcg
      181 cttctaaaaa ttcaggacaa tagtgcttca gcttataact accaacacca tcaatctgaa
      241 tcatttcttg ctttgtgaca ggctttcttt tcgcaaaagc ttctaacgta tagtctgtaa
      301 aaatattcgt cggtggcatg tttaatcgtt cgctcatttc acgtctgact tcgacaagct
      361 tttcaaataa cgtgcggtcg accccttcta cagtgttaat atgcactgtt tctttcgact
      421 tacgcttgaa cggccttgta aacacttgtg tatcctcact caataacttt tctactgagg
      481 catcacacat gagtatttcg tcatgttcgt tgagaaagcc tttaaagcgg agttcatcga
      541 ttaaatgatg taactcgcca gtcgtatagt ctttcatcaa accatacgtc gaaagatttt
      601 catagccttg atgacgaatg tagtccgaaa cctcgccacg caaaacttga atgaccgttt
      661 gatatccttc ttgttgacgt aaacgtgcga caccgctaat gatcattttc gcttctttcg
      721 tcatattgta cgttttattt ttatcaatac aattactaca ttggtgacat tcttcaagtt
      781 tttcattcgg ctcaaaataa tggactaatg ttgcttctag acatttcgtc gttttcgtat
      841 attgaatcat tttcgtcaat ttttcgccca tacgttcctt gtaatcatcg tccgctttcg
      901 aggcaccgat aaaatactga tgtaaagcaa tatctcgatc actgtacaat aaaatacaat
      961 cactatctaa tccgtctcga cctgcacgtc ctgcttcttg ataatacgat tccaaatcac
     1021 ccggcatatt gtaatgaatg acataacgga cattcgattt atcaattccc ataccgaatg
     1081 cgttggtcgc aacgactaca cgagtacggt catataaaaa gtcattctgg gctttatctc
     1141 tttgtttcgc tgtcattcct gcatgatagt aggatacagg gatatctgcg tcttctaaag
     1201 cttcatacaa ggcttctact tgtttacgcg ttgaacaata aacaatcccc gctttctgac
     1261 tatgtttttg aatataatcc aatacaaact tttgacgttg atacgtccca ttcacctgga
     1321 acttcaaatt acgtcgacga atactagttt tgactaagtg ttgtcgcgaa atcattaacc
     1381 gttccataat atcttgttgc acttcagtcg tcgctgttgc agtcaatgcg acaatcgaaa
     1441 actgttgcgg catagtcaat acgcgttgta tgactgcttg ataactcggc cgaaaatcat
     1501 gtccccattt tgaaatacag tgtgcttcgt caaatgcgac gagtgaaata tcgacacgtc
     1561 gcaacaacat ttcaaaataa gtttgctcaa aacgttccgg tgcgatgtat aaaaatttca
     1621 actcaccatt gaccaattgc gtttctatct ctttttgtgc ttgcgtactt aaactactat
     1681 tcaaataagc cgcagatatt cccattgcgg tcaattggtc cacttggtct ttcattaacg
     1741 aaattaaagg actaatgaca atggtcgttc ctttaaacat caatcccggt acttgataac
     1801 ataacgattt acccccacct gtgggtaaca cccctaacgt atgatgatga ttcaatacgc
     1861 gttcaataat ttctttttga ccaggacgaa aagatttgta tccaaaataa tgcgacaatg
     1921 tttgctccat gctatccctc tattgttcta atatttcttc tagttcactc caccgcgtca
     1981 tgtcggtatc atattgagct tcgagcgttt ctttttcttc atttaatgct ttgattttgg
     2041 cataatctgc gttcgcttcg atcatttcgg cgtcaatttc agccagacgc gactctgttt
     2101 cctcgatgcg cactaataac atgtcatatt ctcgtttttc gttgtatgat aatccttttt
     2161 tcttttttgg ttgtaatgtc ggttgttgtt tcacggacgc tacttgagtt gctgcttgtt
     2221 ccaattgttt ttggtatgct aaataatctt caaactgccc taaaatcatg tcaatttgac
     2281 cgtcgtgaat ataccaataa caatccacga ctttatctaa aaagtaacgg tcatggctca
     2341 cagtaatgac ggtaccacca aatgtttgaa tgtagtcttc taaaatcgtt aatgtttctg
     2401 tatccaaatc atttgttggt tcgtctaaca acaacacgtt cggctgatgc actaacaact
     2461 tcaataaata caaacgcttt tgttcgccac ccgagagttt atacactttc ttaccatgcg
     2521 tcacactggg gaataaaaag cgttccaaca attgcgtaat cgaaacggtc gtcccatctt
     2581 tttgacgcgc caactcactt tcttcacgca agaaatcaat gacacggaca tcacgattca
     2641 atcgttcatc cgtttgttta aaatatgcca cttttaccgt ttgacctatc tttaatacac
     2701 cttcaaaatg ctgatcttca cctgctaaaa tgttcagcat cgtcgttttg ccgacaccgt
     2761 ttgggcccac aataccgatt tgaacacctt tttgaatcaa tgtcgacacg ttttcaaata
     2821 atgtacggcc atcaatacgt ttcgtcacat tttcaagttc aaagacttgt ttgcctaagc
     2881 gcgaataagc cagattcaat gaccccttcc cttgcacttg atgcgattgt acttgttctt
     2941 tcaagtcatg aaaacgatct tgccttgctt gttgcttcgt agttcgcgct ttgacaccgg
     3001 cacgcatcca cgctaactct tgtttaaaaa gtgcacgttg tttcgtttgt tgacgcactt
     3061 cgatttcttc ccgctctgct cgcattgcaa tatacgcttc atagttacct ggatatgtat
     3121 ctagcttgcc atttcgcaat tctacaatac gcgtcgccac ttcatttaaa aagtaacggt
     3181 catgcgtgac aaaaacgact gttttcggat aagactttac aaattgaatt aaccaatgaa
     3241 tcgattcgaa atctaaatgg ttcgtcggtt cgtctaataa taataaatct ggtttttcaa
     3301 tcaacgtacg cgctaacact acgcgctttt gttggccacc tgagagttca ctcattcggc
     3361 gtgtcgtatc atggatgcca agtttcgata aaatcgtttt aatctcagca ctataatccc
     3421 aagcactgta agcatccata tcagcttgtg ctgccatcat tgcatcaaac gctttgtcag
     3481 tctgttgttc tgcgtattgt gcagtagctt cttcataacg tttaatgact tgcaactctt
     3541 tggaatctgc actataaacc gcttcataaa ccgttaacgt tggctccaaa ttatgatttt
     3601 gtgccgcata acgaatacga tattgtttcg gatgcgattg aacggcatca aaatcttcat
     3661 ccatgcctgc gatgactttg agcaatgaac ttttccccgt tccattaata ccaactaatc
     3721 cgattcgctc ttgattggaa atggataact gtaaatcatc aaaaatgact ttatccgcat
     3781 aagatttgtt gaggcgttca attttatacg cttccacaac acatacaccc tttttcttat
     3841 agatttactc gcatttatct gttaacctta aatcagttct atatattata cacttttttt
     3901 ggaacaaata atacaaggag ctatttctat gttggattat atttcaaaca tgccgccctt
     3961 tttacaagca ttacttgcag gtgtcatcac gtggttatta accgcattag gggctgcaac
     4021 cgtatttgta tttaaaagtg tcaacgaaaa agtgttgaat tcgatgcaag gctttgctgc
     4081 tggtattatg atcgccgcaa gtttttggtc attactccaa ccggccattg aaagcagttc
     4141 gggcagtact gtaccgtggc tccctgctgc aattggcttt ttactcggcg gggtattcat
     4201 ccgttcttta gactatgtta ttccacacat gcatcgaaac gcaactgatg aatcacaaca
     4261 tcaagaaggt gttgagacga gtctaaataa aaacacactt ttattacttg cgattacatt
     4321 gcataacatt cctgaagggc tcgcaatcgg ggttgccttt ggtggtattg cgacaggaaa
     4381 tgaacacgca actttgttag gggcactcgg acttgcaatt gggattggga ttcaaaacat
     4441 tcctgaaggg gctgcattat cattaccgat acatgcttca ggacagtcca aatggaaagc
     4501 cttcaactat ggacaagctt cagcacttgt agaacctgtt tttgctacag tgggcgcagc
     4561 tgctgtgttg gtcgttactc cgatactacc atacgcactc gcttttgctg caggggcgat
     4621 gatttttgtc gtcgttgaag aattaattcc tgattcacaa gccggtaaaa acacagatct
     4681 tgcgacacta agcttgatgg ttggctttac catcatgatg atattagatg ttgctttagg
     4741 gtaaattcaa aaaaccgttc aatgtgatga aacactgagc ggttttttga tgtgtacata
     4801 tatcgataca ttaaaaatac tctttaacaa ctcacaacaa agatttcaca ctgttgacta
     4861 tataaaatat caaccaatat accaaaatat aataattaac ttaagttata attgtaaaat
     4921 tcagaaaata aaaggtgtga aaaaacagta ccttcatttc gttcttcaca ttggttgtgt
     4981 taatacaaaa aataccatta ttaattaatt aagttagaat agtaaccttt tgcttttaat
     5041 aaaaattcat gtgagccctc ttctataatt tttccttttg ctaaaacaaa tattttatct
     5101 actttttgag ttatatttag attatgggag ataaatataa tagttttatt taacgccatc
     5161 aaattattaa taatttttct ctccgtgagt atatcaatat tactcgttgc ttcatccatt
     5221 atcaatatag aagaatttgt taatagtgct cttgctaaag ctatcttctg ccgctgacct
     5281 cctgacaaag cagaaaaatc agaacttaat acactatcaa gtccaagtgg taagctatca
     5341 ataaaggatt ttagttcaac cattttaata gcactatcta tgtccttttg cgtaacctct
     5401 ttaactaccc ctaataaaag attttcaagt atagttccat taaaaatata agtttgttgt
     5461 ggaatgtata taatctgctg tcgtaaaaca gatttattga tatttgttat gtccaatcca
     5521 cctagtgtta ttttaccctt tgaaggttca aaaaaactaa ccatcaattt cgccaaagtt
     5581 gttttacctg atccagactc tccaataaag gctactttac tattatgttc tattgttata
     5641 ttaatatctg taaggatttc tttttcgtac acatatccat atgaaatatt tgaaaatata
     5701 agtttacttg agttttggtt caatttgata tccaatttat tcttttcaaa ttctgattta
     5761 actgtgaata tttcattcaa tcgattatta gccacacctg ctatttgtaa tttaagctgt
     5821 aaattcataa tattttctat tggattcata aagtaaatca ttaatgaatt aaaaacaagc
     5881 aaagttccaa ttgagatttg attatctata actaggttag cgcctaccca taaaatcgat
     5941 acatttaata ttaattgaac aacctttttc aaaacgtttt gctttatatt tagtttgttt
     6001 aacaagaatg ttttttcgca ataattcgaa aaattatatt ttatctgctt atagcatttc
     6061 ttttcacatg tcagtgcttt tatcgtctcc atacctgtta tattttccat caaataagaa
     6121 gacaaagtag catttgcctc catagatttt attcttttcc gttcaaaaga gttcataaaa
     6181 ccaaaaacaa ttacggcata aataggaatg ataattaata ttaatacaaa taaaatagta
     6241 ctttgataat acaggaagat agatatgcca actattattg taacatctaa aaacaatgat
     6301 agcatggtac tagctaaagc atcagtaatt gaatttgcat cattaagtct tgatagtatg
     6361 tctcctgctc ttcccgtttc aaaaaaagta ataggtaatc taaacaaatg tttaatgtat
     6421 ggtgaaagta tatttataga atatgtcttt actaataaaa tcaaaaaata tttctgcaaa
     6481 taactaagaa tttgttgcat aatgtaagaa gcaattaaag ccatggaaat catactaacc
     6541 catgttttta acttttgggg aatataaaaa tcaataattg acttcataaa atatgaacct
     6601 aaaatattta tcatattgac taaaatggaa atgattatta tttttataat caatcttttt
     6661 ttcttttaat aataaaggta taaaataagt taaacgaact ttatcttgct ttataggctt
     6721 atataaagat gtaggtttta ataagattgt aactccagtc cactcttctt taaattccct
     6781 ataaggcatc tttgttaaat taacaacagg gtcagctata tagatatact ttttatcaca
     6841 tctaattaca acataatagt gaaaatgatt gttatttttt ttaacatgaa cgataaaagg
     6901 atactgtaaa tcatcaattt caaacaaaga tatatctgct ttaataggaa ctgtctcaaa
     6961 acctaactta tctgctacct gaaccaaacc atatgctgta gtgccattta ctgttgttct
     7021 taactttctt ctcagaaaat ccaaagaata gtgagaaccg taaaattcta atatcatagc
     7081 gagcgctgcc gcgccacaat cttgagagtc tacttgtgca cgataaaatc gttttgaaaa
     7141 tttcattcga cacttctact ccttcccacc actttttaga acaacattta aatattcaaa
     7201 aagcgggcgg taattcccac ccacttaata tcatgtcaaa attcaatcct attattaaaa
     7261 gctttaatgg ttattcatct ttacatattt ttttcatata atcacatgca ctctctggat
     7321 cagatgtacc gaatctaacc aatatactgt cgtttataca tgtaatctga gcccatttac
     7381 aacgggacat cccaccactt aagtctttca gcacctgatt tgatatcact ttaaatttat
     7441 caatactttt tatatctatc cctcctaaat agaacaattt aataatacta ttaaaaatca
     7501 attaaatgta tgatttattt tcaaaacgga atctcgttag ttatagaaaa tgggagataa
     7561 tccaaataaa atttgacttt ttctaaatct ttatggtcta tattttttgt tatcattttc
     7621 ttgaattgct cggttgataa ttccgtatgg catatccaac gcttaccact atagctatac
     7681 caagttccta ttactttaaa ttcgtcttta aatagaggag ccctatctaa aagactaaaa
     7741 tcaactatat aaatactttg aaatgttttt tcataaagtt ttttttgaga taattgacat
     7801 aaaattttct ttaaagatat tttaatcata gatttctcac tcctttacac tacctgttat
     7861 cttaatgcgg tagattcctg agctataata atatcaatat ttattagctt aaagtcgata
     7921 gacaatcata aataaatttt ttattataaa aacaaactgt atctttacta tttatttcta
     7981 attcttcaaa tactttttta ctttccttta aatatcctcc ccaaaataaa gaggctccta
     8041 ataacgaatg agtgcattct ttcgaaaaaa ttacatctcc ctcatttaac aaatcataat
     8101 tctctaagaa acttttcaat aaatttgtag catctatatt aagtttcttt atatagtatg
     8161 aatatttcaa aaaaataaag tcttgagtat gattaaaatc tttctctaac agctccatat
     8221 agtaagtaaa acgttcatga ttggattctt ctaataactt aaacatcaaa agaaaaataa
     8281 ttctaacaga gtaataattg ttgtttattt cgattccaga tatcgcatct cttttaaata
     8341 taattttttt acaaaatttt aacatttgat tataatttat atcttctata ttatccctca
     8401 aatataaata ataccaacga ccttcttttg ggaaatctat aattgtttta agaagcaatt
     8461 tattataata atttgttttc ttttgacttc ctcttctaat tcctaaaact tctacattta
     8521 aattagcata agatacagaa ttatttaaag aaaagaatgt ttcgtgtact ctacctttgt
     8581 acgtcaaagt attagtattt cttatcaact tacttgtttt atatacttct tcatctctaa
     8641 ttactttaaa attaaaggcc gtattttcaa agtcgtcgaa aattcttaaa tacgttaagt
     8701 aaaactttaa attatcattt tttctaacaa tttcgtcagc atctaaaatc ataatccagt
     8761 cacaagttgc tttagcattt gcaaagtttc ttgcttttga aaaatcatca caccaattaa
     8821 atgtaaatat ttttattttt ttatcacaca tttttttaat taaatctaaa gtgttgtctt
     8881 cagaaccagt gtcaacaata atgatttcat cggctaaatc taaaatactc aaaaggcttt
     8941 ttataatata atctctttcg tttttaacaa tatacactac agaaattgta gtatattcac
     9001 ttataatctc taatttattt attttttcaa tcaattctgt atcgttttta actcttttgg
     9061 actcccaatc ttttgattct ataaatttat ttaattcttt tattttgttg tatgtttcac
     9121 aagaaatatt aagaaatgcc ttattttttc taaccaatct tcatcaaata tagggtatat
     9181 gtttttcaaa gttaatcaca ccccctcaaa aaaaaataat aaaacaaaat atttttaaaa
     9241 gttttgcaag actgagaggc gaattattat gataaatgcg taattctact atcacactta
     9301 caaattgcta tgctttaatt ataaataatt gtattacttt ttaaatatag tgtccatatt
     9361 tatttatatt aggaagtata atttctcaca tatactttaa ttacagaagt acatgcagtg
     9421 tagatgacgg ttataaaatg taggaaaatg gcgaaatgaa aatgctgctt tttttatata
     9481 aacgccaaga taaatatgga caagtttatt ctttttatga aaggaaatgt tttaatctat
     9541 aatactttcc aacgatagag gcgatcgaga cgggttcttt gtcaacgtcg gttggtgaga
     9601 caataacggc actgagacat tatggtttcc taatctctgc ttctatttat agactgatat
     9661 cgattaataa tgattgatcc ttcgaaaata aggggtgaat gtgtataaaa aatgatgcca
     9721 ttgttttctg gatttcataa tcttgcactc agtttcgctg tattgatata ggattaccca
     9781 gatggctgag acgcctgagg gggaacgtgc atactgttga cagtcgcctc tttactttaa
     9841 tggtgggagg cgtttatcgc agtagcgggc tgacttctca gtgttttcac ttttgagaag
     9901 tctaatcagc catgcaaggc agtacaacga aatcttgttg catatgagat ttctgtactg
     9961 ctcctaaaat ccaatttggc ttcaatcaag ccgaattgag ttgaagggcc ggctctagga
    10021 aagcgaaacc atgatgacaa ttaaaaccac aaatcgtaat gcggacaagt ttaataaaaa
    10081 tattacaaca atagcatcaa aaatttttaa gacccattcc gttttttata tgcacttaca
    10141 agtatcttag aaatgattaa gattccagaa atttctataa ctataagaca cacgtttgtt
    10201 ttattattaa tcctttcaat cgggccattt gttaaatgag gatgatgcat agtatttgag
    10261 gttcagggca aatatttgat gaatgtttgc aacgattagg taaaatgtcg atgatatcat
    10321 gggttacact atcacagtaa ataaagctca ttggtccttc gatatctttc gttgatttga
    10381 attcgtcaaa agctaagtgc tcaggtaaag catcatgtac gtatgtcttt acaaattact
    10441 cacctctttt aaatggcaat gaacagttga aggtgataca catagacttt tagcgacatc
    10501 tttttcagat gtgaatttag ttagcttccg cgtcatcaag cgtttgacct tatttgagac
    10561 ttcggggttg aatatagggt gttttagtta taaaggtttg ttgacaagct ttaaagtaaa
    10621 aacgatgctt cttcaaattt aaataaacag gtctttcaaa attaaaaaac gggcagtaaa
    10681 tctctcacca accgttaaaa atacacaacc aaatatatac aacctaaaaa acagggaaat
    10741 gcactgattt gtacacttcc ctatcataac attttatttt tgatttgcct ttggacctgt
    10801 cggatattta taatctgctg ggttcaattt gttgaaatca ggatttttat agaatcggaa
    10861 taagtctcca ttcaagactt tatcactcat ttctaagtca tgttgtacct gctttttgtc
    10921 cttatcaaga tctgctggcg gtgtttctaa cggttgattg tctctgttac tgtaggcttt
    10981 accgttgaca tatttataat ttctcgtcac gaaatcacca ttacggaatg gtacaatttc
    11041 tttatggtcc ttggataaca tatctgtacc aaacatgaga taatttttcg tatcaatacc
    11101 taacaagtga agtaatgttg gcatcacatc aacttgacct gcatacgtgt cgtccacttt
    11161 aggctcaacc cctggtgctt ttaaccagaa acccgttgca ttcaagtcat taaacttcgc
    11221 tggtgtaatc tcttcaccta ataactgtga catcgctttg ttatgatttt ctgagatacc
    11281 ataatggtca ccgtaaatca taatgactga atcatcgtac agaccacgct ctttcaagtc
    11341 attaatgaac tgttctaacg actcatctaa gtaacgcgct gtttgaatgt aaccatcgac
    11401 cgttgagtta cctgtgtcac cttttgcaat cgttgcgtct tcatcactta atgtaaaagg
    11461 ataatggttc gtcaacgtaa ttaaatgtga atagaacggt tgtttttctt tagataagta
    11521 atccgccgcc tctttgaaaa actctttatc ttttaatcca aggttttcta agttttctgg
    11581 agacatgtca aaatatgttg catcataaaa ctta
//
