LOCUS       NZ_CP048882            10402 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Streptomyces bathyalis strain ASO4wet chromosome, complete genome.
ACCESSION   NZ_CP048882
VERSION     NZ_CP048882.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces bathyalis
  ORGANISM  Streptomyces bathyalis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7377472)
  AUTHORS   Risdian,C., Landwehr,W., Rohde,M., Schumann,P., Hahnke,R.L.,
            Sproer,C., Bunk,B., Kampfer,P., Schupp,P.J. and Wink,J.
  TITLE     Streptomyces bathyalis sp. nov., an actinobacterium isolated from
            the sponge in a deep sea
  JOURNAL   Antonie Van Leeuwenhoek 114 (4), 425-435 (2021)
   PUBMED   33595745
REFERENCE   2  (bases 1 to 7377472)
  AUTHORS   Risdian,C., Landwehr,W., Schupp,P. and Wink,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2020) Microbial Strain Collection, Helmholtz
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig,
            Niedersachsen 38124, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-FEB-2019
            Assembly Method                   :: HGAP v. v3
            Assembly Name :: Streptomyces_DSM106605_HGAP3_1SC_10M
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117.0x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910445.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 04:35:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,467
            CDSs (total)                      :: 6,387
            Genes (coding)                    :: 6,276
            CDSs (with protein)               :: 6,276
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 37 of 111
            Pseudo Genes (incomplete)         :: 90 of 111
            Pseudo Genes (internal stop)      :: 12 of 111
            Pseudo Genes (multiple problems)  :: 25 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:44:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6288945
            Orig. end                         :: 6299347
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from MISG, Helmholtz Centre for
            Infection Research, Germany.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10402
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="23"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10402
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10402
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5402](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5402)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    131..144
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.5"
                     /tool="antismash"
     gene            complement(484..1254)
                     /locus_tag="G4Z16_RS27225"
                     /old_locus_tag="G4Z16_27195"
     CDS             complement(484..1254)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381154.1"
                     /locus_tag="G4Z16_RS27225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_27195"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_028435560.1"
                     /transl_table=11
                     /translation="MSAGETGASQVKSAVRTVELLEYFAGRPGMHSLADVQEAVGYPKS
                     SLYMLLRTLVDLGWVETDSTGTRYGIGVRALLVGTSYIDGDEAVAAARPTLDRLSDDTT
                     ETIHMARLDGTNVVYLATRQSQHYLRPFTRVGRRLPAHSTSLGKALLATYDDEQVRKML
                     PETLPSLTGNTLTDREALIEELALVREQGYAVDREENTLGLRCFGVAIPYRTPARDAIS
                     CSVPVARLTPAHEQMIKDALFDARDRLTLATRRL"
     gene            1375..2907
                     /locus_tag="G4Z16_RS27230"
                     /old_locus_tag="G4Z16_27200"
     CDS             1375..2907
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987789.1"
                     /locus_tag="G4Z16_RS27230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_27200"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_197353266.1"
                     /sec_met_domain="Aldedh (E-value: 2.1e-44, bitscore: 150.4,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAAPVWSVDPRTGKQREQVGTEATAGEVDAAVRAAHAAREPLAD
                     RTVRAALLRAAAGQLDAASEEIIVTADAETALGRPRLTGELARTQAQLRAFADEIEDGA
                     YLGITINHKDADATPPCPDLRRYKIPLGVVAVYAASNFPLAFSVPGGDTASALAAGCPV
                     VVKAHPDHPATSELTGAALRRAAAEVGLPEEVLSVVHGFDAGVELVKHPLVSAVGFTGS
                     VRGGRALFDAATARPTPIPFHGELGSLNPVVVTGAAAAERAEEIGEGLAGSMTMGTGQF
                     CTKPGFVLAPQGEAGDRMLEALRVAVSKAAQGPLLDSRMREAFLAGVGARTELEGVEAP
                     VLAGAGDDEQSVGAGFLTVPAEKLGQPQYELLLEECFGPVTVVARYQDDDEVVSALSRL
                     PGNLTATLHLGAAEAAGDGGQGARLLATLTPLAGRVLVNGWPTGVAVAPAQHHGGPYPA
                     TTSTSTSVGATAIERWLRPVAYQDAPEALLPPELRDANPLGLPRRVDGRREN"
     gene            complement(3010..3858)
                     /locus_tag="G4Z16_RS27235"
                     /old_locus_tag="G4Z16_27205"
     CDS             complement(3010..3858)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090397.1"
                     /locus_tag="G4Z16_RS27235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_27205"
                     /product="urea carboxylase-associated family protein"
                     /protein_id="WP_197353267.1"
                     /transl_table=11
                     /translation="MASTAAAYQSDEGGPLDVDRAFYDRVAAGPREPVERFTVPIRSGR
                     AWEVPAGHLCRLVTVDGPQVADLNVWNRHDPRERLWASRTRQLQRAHVSVYDRLWSTLP
                     FLRPLLTVTADTLASYGRDAEGGRVHDLLGTRCDPYVNRMLTGEDFDFHCHSNLVRAVL
                     PYGLTEFDVHDVLNVFQCTGLNEDDQYFMKDCPARPGDHFEFFAEIDLLCAISTCPGGD
                     LSVPMWGPGSDVDPVEFCHPVGVEVHRADPALLTDWAPPEPAAYRGMHGMAFPLAPGGR
                     S"
     gene            complement(3995..4894)
                     /gene="thpD"
                     /locus_tag="G4Z16_RS27240"
                     /old_locus_tag="G4Z16_27210"
     CDS             complement(3995..4894)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="G4Z16_RS27240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_27210"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_197353268.1"
                     /sec_met_domain="PhyH (E-value: 1.3e-38, bitscore: 131.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGATEVATPRMDPVVWSEPGTPGPIEAAVLA
                     EYERDGFLAIDQLITPDEVDMYSRELERLTTDAEIRADERSIIEPQSDEIRTVFEVHKI
                     SEVFADLVRDPRVVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHAEDGLP
                     AMRTVSVSIALTRNYDTNGGLMIMPGSHKTFLGCAGETPKDNYKQSLQMQDAGTPSDES
                     LAKFADAHGIKLFTGEAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENKAVEPFA
                     APVRRPEFIGARDFTPVR"
     gene            complement(5001..5402)
                     /locus_tag="G4Z16_RS27245"
                     /old_locus_tag="G4Z16_27215"
     CDS             complement(5001..5402)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907220.1"
                     /locus_tag="G4Z16_RS27245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_27215"
                     /product="ectoine synthase"
                     /protein_id="WP_197353269.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.4e-51, bitscore:
                     172.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEDTDRHIKSASGTWESKRIVLAKERAGFSLHETVLY
                     AGTETSMWYANHIEAVLCVEGESELTNDETGEKHLITPGTMYLLDGHEKHTMRPITDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPDD"
     gene            complement(5481..6749)
                     /gene="ectB"
                     /locus_tag="G4Z16_RS27250"
                     /old_locus_tag="G4Z16_27220"
     CDS             complement(5481..6749)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-365). E-value: 5e-75.
                     Score: 244.5. Matches aSDomain:
                     nrpspksdomains_G4Z16_RS27250_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="G4Z16_RS27250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_27220"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_197353270.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.9e-78, bitscore:
                     260.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIIQPDLSVFETVESEVRSYCRGWPTIFDRAQGSHMYDEDGHTY
                     LDFFAGAGTLNYGHNNPVLKRALLDYLERDGVVHGLDMSTTAKRAFLETFKEKILEPRG
                     LNHKVMFPGPTGTNAVEAALKLARKVKERESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDDYLGGTTEDFVWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRDLSDLCERHDMLLIVDDIQMGCGRTGAFFSFEEAGIKPDIITLSKSIGGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTATAAFETYWTDDQTEKQTLSRGEQVEAVLR
                     AISEEHSEDVADFRGRGLVWGLEFHDKDRADAVAKRCFELGLLIETSGPQSEVVKLLPA
                     LTITAEELDEGLRVLARAVRETA"
     aSDomain        complement(5655..6662)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G4Z16_RS27250_Aminotran_3.1"
                     /evalue="5.00E-75"
                     /label="G4Z16_RS27250_Aminotran_3.1"
                     /locus_tag="G4Z16_RS27250"
                     /protein_end="365"
                     /protein_start="29"
                     /score="244.5"
                     /tool="antismash"
                     /translation="DRAQGSHMYDEDGHTYLDFFAGAGTLNYGHNNPVLKRALLDYLER
                     DGVVHGLDMSTTAKRAFLETFKEKILEPRGLNHKVMFPGPTGTNAVEAALKLARKVKER
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDDYLGGTTEDFVWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRDLSDLCERHDMLLIVDDIQMGCGRT
                     GAFFSFEEAGIKPDIITLSKSIGGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFVT
                     ATAAFETYWTDDQTEKQTLSRGEQVEAVLRAISEEHSEDVADFRGRGLVWGLEFH"
     gene            complement(6876..7454)
                     /gene="ectA"
                     /locus_tag="G4Z16_RS27255"
                     /old_locus_tag="G4Z16_27225"
     CDS             complement(6876..7454)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064039.1"
                     /locus_tag="G4Z16_RS27255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_27225"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_197353271.1"
                     /transl_table=11
                     /translation="MTAAQADRARAHSDFREMPEGLTLDAPQLEDGAALWRIARDSRTL
                     DLNSSYSYLLWCRDFAATSVVARDEGGEPVGFITGYVRPARPRTLVVWQVAVDEAARGR
                     GLAAAMLDGLTARTARELGIDGIETTVTPDNAPSNRLFTSFAERHGAATEKEVLFDAGV
                     FPESGHEPEVLYRIGPVASLKDRAPLTVH"
     misc_feature    complement(7455..7471)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     misc_feature    complement(7610..7625)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7620..7635
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7620..7637)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.31"
                     /tool="antismash"
     gene            7891..8982
                     /locus_tag="G4Z16_RS27260"
                     /old_locus_tag="G4Z16_27230"
     CDS             7891..8982
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468153.1"
                     /locus_tag="G4Z16_RS27260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_27230"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_197353272.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.5e-16, bitscore:
                     59.1, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDRGNLHIKGRVLAGPEDIRDELWVVDGHVTYERPAGSDRDTVR
                     AEGWVLPGLVDAHCHVGLDQQGPVDESTTEKQALTEREAGALMLRDAGSPSDTRWIDDR
                     EDLPRIIRAGRHIARTKRYIRNYAHEIEPGDLAAYVRLEARRGDGWVKLVGDWIDRGEG
                     DLAPCWPRWALEEAIAAAHEEGARVTAHCFAENSLRDLVEAGIDCIEHATGLTEETIPL
                     FAERGVAIVPTLVNTATFPQLAEGGTKYPRWSEHMVRLYERRYETVRAAFDAGIPVYTG
                     TDAGGSLAHGLVPVEVAELVTAGIPPADAISAATWGARRWLGRPGLEEGAPADLLVFAE
                     DPRADVGVLTDPRHVVLRGRVIR"
     misc_feature    9830..9839
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     misc_feature    complement(9931..9940)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
ORIGIN
        1 cgctgtacaa catctttgcg acctgcggca gttgatgccg cagcgagcag tggatgaagc
       61 cgacctcttc gagggtgcgg ccacgcgtgg acatctcgta cgtgccggcc gcgcgtgcgg
      121 cctcccacga ggaacgctcc gtgatgtgca gcaactcagc catgaggcaa cggtagccgt
      181 cgcaagctct caccggggtt cgcggacagg cccccgggcc ggaggcactg cacatacgcg
      241 ccgcccgccc cggcatcgcc atcccggagc cggagccgga gcagactccc tgccctgtga
      301 gttgcccggg tcaacgcccg ccgggggtga cgaagcccgg ctcgtaggcg gcgatcacgg
      361 ccgtccccat gggcgggtga gggcgagaac gagcacgacg acgaggcgtc caccacgatc
      421 gtcagcgccc tccgccgcgc ggcgcgggcg tcctcgtccc ggggcccgcg ccgcggcccg
      481 gctctacagc ctgcgggtgg cgagcgtcag ccggtcgcgg gcgtcgaaga gcgcgtcctt
      541 gatcatctgc tcgtgcgcgg gcgtgagcct ggccaccggg accgagcagc tgatggcgtc
      601 ccgcgcgggc gtccggtagg ggatcgcgac gccgaagcag cgcagcccga gagtgttctc
      661 ctcgcggtcg acggcgtagc cctgctcgcg caccagggcc agctcctcga tcagcgcctc
      721 gcggtcggtg agggtgttcc cggtgagcga gggaagcgtc tccggcagca tcttgcgcac
      781 ctgctcgtcg tcgtacgtcg cgagcagcgc cttgcccagg gacgtcgagt gcgccggcag
      841 ccgccgcccg acgcgcgtga acggacgcag atagtgctgc gactggcggg tggcgaggta
      901 gacgacgttc gtgccgtcca gccgcgccat atgaatggtc tccgtggtgt cgtcggagag
      961 ccggtcgagg gtgggccggg ccgccgccac cgcctcgtcg ccgtcgatgt acgaggtgcc
     1021 gaccaggagg gcgcgaacgc cgatgccgta gcgtgtgccg gtggagtccg tctccaccca
     1081 gcccagatcg acgagggtgc gaagcagcat gtagagactg gacttggggt accccaccgc
     1141 ctcttggaca tcggcgaggg agtgcatccc gggccgaccc gcgaagtact cgagcagctc
     1201 cacagtgcgc acggcggact tgacctgcga tgcacccgtc tcgccagctg acatcgtcct
     1261 tgacccctct gttgcggcgc tcataggctc cccggcaggt tcactgacag ggatggtgtt
     1321 cagcatatcg aacgccgtct ctgcggagac aagtacgtgg caggaggaac cgctgtggca
     1381 gcagcaccag tgtggagtgt cgatccccgg accggaaagc agcgtgaaca ggtcggcacc
     1441 gaggccacgg ccggtgaggt ggacgcggcc gtcagggccg ctcacgccgc cagggagccg
     1501 ctcgccgacc ggaccgtgcg cgccgccctg ctccgggcag ccgccggaca gctcgacgcg
     1561 gcgagcgagg agatcatcgt caccgcggac gccgagaccg ccctgggacg cccgcggctg
     1621 accggcgagc tggcccgaac ccaggcccaa ctgcgcgcct tcgccgacga gatcgaggac
     1681 ggcgcgtacc tcggcatcac catcaaccac aaggacgccg acgcgacgcc gccgtgcccc
     1741 gacctgcgcc gctacaagat tccgctcggt gtcgtggccg tctacgccgc gtccaacttc
     1801 ccgctggcct tctccgtgcc cggcggcgac accgcgagcg cactcgcggc gggctgcccc
     1861 gtcgtggtca aggcgcaccc ggaccacccg gcgacgtccg agctgacggg cgccgcgctg
     1921 cgccgcgccg ccgccgaagt gggcctgccg gaagaggtgt tgagcgtcgt gcacggcttc
     1981 gacgccggcg tggaactggt caagcacccg ctggtctcgg ccgtcggctt caccggctcc
     2041 gtacggggcg ggcgcgccct gttcgacgcc gccaccgccc ggcccacgcc cattcccttc
     2101 cacggcgaac tcgggtccct caaccctgtc gtcgtcaccg gcgccgccgc cgccgagcgc
     2161 gcggaggaga tcggtgaggg gctcgccggg tcgatgacga tgggcaccgg ccagttctgc
     2221 accaagccgg gcttcgtgct cgcaccccag ggcgaggcag gcgaccgcat gctggaggcg
     2281 ctgcgggtcg cggtgagcaa ggccgcgcag ggaccgctgc tggacagcag gatgcgggag
     2341 gcgttcctcg cgggggtcgg ggcacgcacg gagctggaag gagtggaggc gcccgtgctc
     2401 gccggtgcgg gtgacgacga gcagtcggtc ggcgccggct tcctcacggt cccggcggag
     2461 aagctcggac agccgcagta cgaactgctc ctggaggagt gcttcggccc cgtcaccgtc
     2521 gtggcgcgct accaggacga cgacgaggtc gtctcggctc tctcgaggct gcccggcaac
     2581 ctcaccgcca cgctgcacct cggcgccgcc gaagcggcgg gagacggcgg ccagggagcg
     2641 cggctgctcg ccacgctgac gccgctggcc ggccgcgttc tcgtcaacgg ctggcccacc
     2701 ggcgtcgccg tcgcccccgc gcagcaccac ggcgggccgt acccggccac gacgtcgacc
     2761 tcgacgtccg tcggcgccac ggccatcgag cgctggctgc gccccgtcgc ttaccaggac
     2821 gcgcccgagg cgctgctgcc gcccgagctg cgtgatgcga acccactggg tctgccgcgc
     2881 cgggtggatg gccgcaggga gaactgaacg gcagccgtgc gtccctgagt ccggcggagc
     2941 gccctcaact ccgaggagaa ccagcggagt tcagggcccg gactcagggg ggggcggcgg
     3001 ctcaggcgtt caggagcgcc ccccgggggc cagggggaag gccatgccgt gcatgccgcg
     3061 gtacgcggcc ggctccggtg gtgcccagtc cgtcagcagg gcggggtcgg cgcggtggac
     3121 ctccacgccg acgggatggc agaactccac ggggtccacg tccgaaccgg gcccccacat
     3181 cggcacggac agatcgccgc ccggacatgt cgagatcgcg cacagcaggt cgatctcggc
     3241 gaagaactcg aagtggtcgc cggggcgcgc cgggcagtcc ttcatgaagt actggtcgtc
     3301 ctcgttgagc ccggtgcact ggaagacgtt cagcacgtcg tgcacgtcga actcggtgag
     3361 cccgtacggc agtacggccc gcaccaggtt cgagtggcag tggaagtcga agtcctcgcc
     3421 ggtcagcatg cggttgacgt acgggtcgca gcgcgtgccc agcaggtcgt gcaccctgcc
     3481 gccctcggcg tcacgcccgt aggaggccag cgtgtccgcg gtgacggtca gcaggggacg
     3541 caggaacggc agcgtggacc acagccggtc gtacacgctc acgtgtgcgc gctgcaactg
     3601 ccgtgtacgg gaggcccaca ggcgctcgcg cggatcgtgc cggttccaca cgttcaggtc
     3661 cgcgacctgg gggccgtcga ccgtcaccag gcggcacaga tgccctgcgg gcacctccca
     3721 cgcgcggcct gagcgtatgg gcacggtgaa gcgttcgaca ggttcccggg ggccggcggc
     3781 gaccctgtcg tagaaggcgc ggtcgacgtc gagcgggccg ccctcgtcgg actggtaggc
     3841 ggcggcggtg ctggccatgc gcgcggactc tccctcggtc ggactcccgg actgtgcagg
     3901 gagatcgcag cagaaagcgc ccggacggaa aaggggggag gccgtcatgc ggtctccccc
     3961 ctcatccacc gggctcagtg cgccgcgtaa cgcgtcacct caccggggtg aagtcgcgcg
     4021 ctccgatgaa ttcgggacgg cggaccggtg ccgcgaaggg ctccacggcc ttgttctcca
     4081 cgctgttgaa gacgatgaac acgttgctcc gcgggtacgg agtgatgttg tcaccgctcc
     4141 cgtgcatgca gttgcagtcg aaccaggtcg ccgagccggc ctcgcccgtg aagagcttga
     4201 tgccgtgcgc gtcggcgaac ttggccagtg attcgtcgga cggagtgccc gcgtcctgca
     4261 tctgcagcga ctgcttgtag ttgtccttcg gcgtctcgcc cgcacaaccg aggaaggtct
     4321 tgtgtgaccc cggcatgatc atcaggccgc cgttggtgtc gtagttccgg gtcagcgcga
     4381 tcgagaccga gacggtccgc atcgccggaa gcccgtcctc cgcgtgccag gtctcgaagt
     4441 cggagtgcca gtagaagcca gtggcaccga agcccggctt gacgttgatc ctggactggt
     4501 ggacgtagac gtccgagccc aggatctgtc gcgcccggcc cactacgcgc gggtcccgca
     4561 ccaggtcggc gaagacctcg ctgatcttgt gcacctcgaa cacggtccgg atctcgtccg
     4621 actgcggctc gatgatcgag cgctcgtcgg cacggatctc cgcatccgtg gtgagccgtt
     4681 ccagttccct gctgtacatg tcgacctcat caggggtgat gagctggtcg atcgcaagga
     4741 acccgtcccg ctcgtattcc gccagaaccg cggcctcgat gggccccggc gtacccggct
     4801 ccgaccacac gacggggtcc atccgtgggg tggccacctc ggtggctccg cgagtcgggt
     4861 acaggtcggc ggtgcgctcg ggtgcggtgg tcatggtgtt gccttcctct cctctcgtac
     4921 ggccctgttc ggtgggcgag cggccgtagc cgcggacggc agaagccgcc gtactggcct
     4981 gtgcaggccg agctctcggc tcagtcgtca ggctcggtga gaagcgggta gacgccgttc
     5041 tcgtcgtgat cctcccggcc cgtcacgggc ggattgaaga cacaaatgca ccggaagtcc
     5101 gtaatgggcc gcattgtgtg cttctcgtgc ccgtcgagca gatacatggt gccgggagtg
     5161 atcagatgct tctcgccggt ctcatcgttg gtgagttcgg actcgccctc gacgcagagt
     5221 acggcttcga tgtggtttgc gtaccacatc gatgtctccg tccccgcgta cagcacggtc
     5281 tcgtgaaggg agaagccggc acgctccttc gccagcacga tccgcttgct ttcccaggta
     5341 ccggatgcgg atttgatgtg acggtcggtg tcctcgatgt ccttgaaaga acgaacaatc
     5401 acgttgtggc gtacctttct ctattcatta cggggtcggc ccgccccgga cgcaaccggg
     5461 gcgggccgtg agcggatcag ttacgcggtc tcgcgcaccg ctcgggccag cacgcggagc
     5521 ccttcgtcga gctcctcggc cgtgatcgtg agagcaggca gcagcttcac gacctcgctc
     5581 tggggccccg aggtctcgat gagcaacccg agctcgaagc accgcttggc gacggcgtcg
     5641 gcacggtcct tgtcgtggaa ctccagtccc cacaccagac cgcgcccgcg gaagtctgcg
     5701 acatcctcgc tgtgttcctc ggagatggcg cgcaaaaccg cctcgacctg ctcaccacgg
     5761 gagagagtct gcttctccgt ctggtcgtcg gtccagtacg tctcgaacgc ggccgtggcc
     5821 gtgacgaacg ccgggttgtt gccgcggaag gtgccgttgt gctcgcccgg ctcccagatg
     5881 tccagctcgg gcttgaacag cgtgagagcc agcggcagtc cgtagccgcc gatggacttg
     5941 gacagcgtga tgatgtccgg cttgatgccc gcctcctcga aggagaagaa ggcgccggtg
     6001 cggccgcagc ccatctggat gtcgtcgacg atgagcagca tgtcgtggcg ctcgcacagg
     6061 tcggagaggt cacgcagcca ctcggcgcgc gcgacgttga tgccgccctc gccctgcacg
     6121 gtctccacga tcacggccgc cggcttgttg aggccggatc cctggtcctc gagcagccgc
     6181 tcgaaccaga cgaagtcctc cgtggtgccg cccaggtagt cgtcgaacgg catcggcgtg
     6241 ccgtgcacga gcgggatgcc cgcaccggcg cgcttgaagg cgttgccggt gacggcgagc
     6301 gagcccagcg acatgccgtg gaaggcgttg gtgaaagaga cgatggactc gcgctccttg
     6361 accttgcggg ccagcttcag cgccgcctcg acggcgttgg tgcccgtcgg gcccgggaac
     6421 atgaccttgt ggttcaggcc gcggggctcg aggatcttct ccttgaaggt ctccaggaag
     6481 gcacgcttgg ccgtggtgct catgtcgagc ccgtggacga ccccgtcacg ctccaggtag
     6541 tccagcaggg cccgtttgag cacggggttg ttgtggccgt agttgagcgt gccggctccg
     6601 gcgaagaagt ccaggtacgt atgaccgtcc tcgtcgtaca tgtgtgagcc ctgggcccgg
     6661 tcgaagatcg tgggccagcc acggcagtag ctgcggactt ccgactcgac ggtctcgaag
     6721 acactgaggt cgggctggat gatggtcacg gcgtgctcct gggagtgagt tacatgcggt
     6781 cgaagtggtc gggatgcgga tcggatcgcg ggactgcggg gatcgctgac ggttcggaga
     6841 tgtggggcac gaagcgccgg cggaacgcgg cagtctcagt gcacggtcag tggagccctg
     6901 tccttcaggg acgccaccgg gccgatccgg tagaggactt cgggctcgtg gccgctctcg
     6961 gggaacactc cggcatcgaa gagcacctcc ttctccgtcg ccgcgccgtg gcgctccgcg
     7021 aaggaggtga agaggcggtt cgagggagcg ttgtcggggg tgacggtggt ctcgatcccg
     7081 tcgatcccca gttcccgggc cgtacgggcg gtcagcccgt cgagcatcgc cgccgccagt
     7141 ccccggccgc gcgccgcctc gtcgacggcc acctgccaga cgaccagtgt cctcgggcgt
     7201 gcggggcgca cgtagccggt gatgaatccg accggctcac cgccctcgtc acgggccacc
     7261 acggaggtgg cggcgaagtc gcgacaccac aggaggtagc tgtaggagga gttgaggtcg
     7321 agcgtccgcg agtcgcgggc gatgcgccag agcgccgctc cgtcctcgag ctgcggagcg
     7381 tccaacgtca gcccctccgg catttcccgg aaatcactgt gggcacgtgc acggtctgct
     7441 tgcgcggcgg tcatgggtat taaatttacc cagcagaatc cgaaaacgca cggcggggtg
     7501 gggttacaca agaggggggt cgtgtgttat cgcgcgcagg cgcacacgtg agtcaggggt
     7561 aggggttcgc cctgatattt ccgcattttc cagggccaac caggccgcct gtggagtccg
     7621 tcacattgcc gtaacgacct tgaaccctgg gcgaattccg cctcgataac cctgtgaaaa
     7681 atcctggcgt ttaaagaaac ggaaagcggg aagaagaata cgggaagcta cgctcattat
     7741 agatgcggaa atgcattctc ccgcaacggc tcggatgtcg catcttttct tcccggactc
     7801 gcgggaatga ccagcgtgtg catgcctgcc accgtagcgc ccgtgccggg cgggtgcagg
     7861 aacaccggtg cgggctcata gggtgcgggc atgagcgacc gcggcaatct ccacatcaag
     7921 ggacgcgtac tggcagggcc cgaggacata cgggacgagc tgtgggtggt ggacgggcac
     7981 gtcacctacg agcgccccgc cggcagcgac cgcgacaccg tcagggccga gggctgggtg
     8041 cttcccggac tcgtcgacgc gcactgccac gtcggcctcg accagcaggg cccggtggac
     8101 gagtccacca ccgagaagca ggccctgacc gagcgcgaag ccggggccct gatgctccgc
     8161 gacgcgggct cgccgtccga cacccggtgg atcgacgacc gcgaggacct gccgcgcatc
     8221 atccgcgccg ggcgccacat cgcccgtacc aagcgctaca tccgcaacta cgcccatgag
     8281 atcgagcccg gcgacctggc ggcatacgtc cggctcgagg cccggcgcgg cgacggctgg
     8341 gtgaagctcg tgggcgactg gatcgaccgc ggcgagggcg atctggctcc ctgctggccc
     8401 cgctgggcgc tggaggaggc catcgccgcc gcacacgagg agggggcccg cgtcaccgcg
     8461 cactgcttcg ccgagaactc gctccgtgac ctggtcgagg ccggcatcga ctgcatcgag
     8521 cacgccaccg gactcaccga ggagacgatc ccgctgttcg ccgagcgagg tgtcgcgatc
     8581 gtccccacgc tcgtcaacac cgcgacgttc ccgcagctgg cggagggcgg caccaagtat
     8641 ccgcgctggt cggagcacat ggtgcggctc tacgagcgcc gctacgagac ggtgcgcgcc
     8701 gccttcgacg cgggtatccc cgtatacacg ggcacggacg ccgggggctc cctggcgcac
     8761 gggctcgtcc ccgtcgaggt cgcggagctg gtcacggccg ggattccgcc cgcggacgcg
     8821 atctcggcgg ccacctgggg cgcgcgccgc tggctcggac ggccggggct ggaggagggc
     8881 gcgccggcgg acctgctggt cttcgccgag gacccgcgtg ccgacgtagg ggtgctgacc
     8941 gatccgcgcc acgtggtgct gcgggggcgg gtgatccgtt aggcgctgcg gcgggggagc
     9001 gggccgcgtg cggcaccgcg ctcggtgagc accgggagga ccggcaggga cggcgaagag
     9061 gagggagggg agccacgtga aggagatcgt ttcgtgggac gcgatcgagc tgtcccgtgc
     9121 catcgcggac cgcgatgtct cgtgcgtcga ggtcatggag gcgtacctgg agcacatcgg
     9181 cacggtcaat ccgcacgtca acgccatcgt cgccacgcag gacccggaga tctccctgcg
     9241 gcaggcgcgt gagcgcgacg agcagctgcg gcagggccgg cacctgggct ggatgcacgg
     9301 gttcccgcac gccgtgaagg atctgaccga ggccgacggg ctgccctgga ccgagggttc
     9361 acccgtgcac aaggaccgcg tggcgcagca ggacgcgctg ttcgtgaagc gcatcaagtc
     9421 tgcgggcgcc gtggtgatcg gcaagacgaa cacccccgaa ttcgggctgg gttcgcatac
     9481 gtacaacagc gtctggggga cgacgggcaa cgcctacgac ccctcccgca cggcaggcgg
     9541 cagcagcggc ggtgcggcgt cggcgctcgc gctgcggatg ctgccggtgg cggacggaag
     9601 cgactacatg gggtcgctgc gcaatcccgc ggccttcaac aacgtgctgg gcatgcgccc
     9661 gagttggggc ctggtgccct cgcccggctt ctccacgcag ctgagcaccg ccgggcccat
     9721 ggcgcgtacg gccacggacc tggcgtggct gctgtccgtc atggcggggc ccgacgaggg
     9781 cgcccccctg ggcggcgccg aggaaggcgg acggttccgg gagagcctcg aaggcggcga
     9841 cttccgcggc gcccgcatcg cctgggtcgg cgactggggc ggccggctgg cgacggagcc
     9901 cggcgtgctg gaggtgtgcc gctcgtcctt cgccgccttc gaggccctgg ggtgcgaagt
     9961 caccgaggct cggcccgact tcccgctgga ggagatctgg cgcgcgttcc tgacctggcg
    10021 cgggctcgcg aacctcgagc ggcacagctt gtacgggaac ccggagaccc gcgggcgcat
    10081 gaagccggag gcggtctggg agatcgaacg cgcgctggag ctcggtgtcc tcgacgtcgc
    10141 cgccgcggcc gactcgcggg accggtggtt cgcggcgctg accgagttcc tcgcgggcca
    10201 cgactacatc ctcgccccgg ccgcgcaggt gttcccgttc gacaaggaag tccactggcc
    10261 cgagcgggtc ggcggtcggc ggatggacac ctaccaccgg tggatggaga ccgtggtgcc
    10321 ctggacgctc gccggggtgc ccgcgatcgc catgcccgcc gggttcggtg cggacgggct
    10381 gccgatgggc gtccagctca tc
//
