LOCUS       NZ_CP048882            10204 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Streptomyces bathyalis strain ASO4wet chromosome, complete genome.
ACCESSION   NZ_CP048882
VERSION     NZ_CP048882.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces bathyalis
  ORGANISM  Streptomyces bathyalis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7377472)
  AUTHORS   Risdian,C., Landwehr,W., Rohde,M., Schumann,P., Hahnke,R.L.,
            Sproer,C., Bunk,B., Kampfer,P., Schupp,P.J. and Wink,J.
  TITLE     Streptomyces bathyalis sp. nov., an actinobacterium isolated from
            the sponge in a deep sea
  JOURNAL   Antonie Van Leeuwenhoek 114 (4), 425-435 (2021)
   PUBMED   33595745
REFERENCE   2  (bases 1 to 7377472)
  AUTHORS   Risdian,C., Landwehr,W., Schupp,P. and Wink,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2020) Microbial Strain Collection, Helmholtz
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig,
            Niedersachsen 38124, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-FEB-2019
            Assembly Method                   :: HGAP v. v3
            Assembly Name :: Streptomyces_DSM106605_HGAP3_1SC_10M
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117.0x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910445.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 04:35:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,467
            CDSs (total)                      :: 6,387
            Genes (coding)                    :: 6,276
            CDSs (with protein)               :: 6,276
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 37 of 111
            Pseudo Genes (incomplete)         :: 90 of 111
            Pseudo Genes (internal stop)      :: 12 of 111
            Pseudo Genes (multiple problems)  :: 25 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:44:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4551156
            Orig. end                         :: 4561360
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from MISG, Helmholtz Centre for
            Infection Research, Germany.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10204
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="17"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10204
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10204
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5204](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5204)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1309..3426)
                     /locus_tag="G4Z16_RS19835"
                     /old_locus_tag="G4Z16_19810"
     CDS             complement(1309..3426)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576114.1"
                     /locus_tag="G4Z16_RS19835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_19810"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_246531333.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.5e-35, bitscore:
                     120.4, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEAVECGAAALSMVLGYFGRHVPLEQLRIACGVSRDGSRASNLLK
                     AARSYGLTAKGMQMEPAALAEVPGPAVLFWEFNHYVVYEGMGRRFGKRGVHINDPDKGR
                     RFVPLDEFDTSFTGVVLTFEPGEDFRRGGKRPSAMSAMPARLRRTTGTMLAALLASLLL
                     VGVGAAVPALSRTYIDMFLIGDQTSLLGVLFASMGAMVVLTAALTALQQANLLRGRIIS
                     STLSSARFFRHLLRLPVTFFAQRSPADLVQRLQSNDTVAETLSRDLAAAGVDAIVVILY
                     AVLLWTYDPQLTVIGVLIALLNLVAMRVVIRLRATGTQKLRADTARLTNTSYTGLQLIE
                     TMKATGGENGYFRRWSGQHATTLEEQQKLGVPTAWLAVVAPTLATLNSAMILWIGGMRA
                     VEGHISIGLLVAFQALVTRFTAPITRLNGVAGRIQDFAADVARLKDVENFPVDSLYSRR
                     EPIADTRRLKGHVSLESITFGYSPLDKPLLTDFSLSVGPGRQVALVGGSGSGKSTVSRL
                     ISGLYSPWEGVIRIDGQRLEDISRSALAASVSFVDQDVFLFEGTVRDNVALWDPSISDE
                     AVTAALRDAAVLDDFTRRPGGIHSRVEQDGRNLSGGQRQRLEIARALVRRPSILVLDEV
                     TSALDAQTEQVIIDNLRRRGCACVVIAHRLSTVRDSDEIVMLDHGSIVERGRHEELVAR
                     GGPYAELVKEH"
     gene            complement(3915..4721)
                     /locus_tag="G4Z16_RS19840"
                     /old_locus_tag="G4Z16_19815"
     CDS             complement(3915..4721)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="G4Z16_RS19840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_19815"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_197352063.1"
                     /transl_table=11
                     /translation="MQFRQKAFSKLQSPEELDLPVRYARPQGWLVLLVTVLVMAGAGYW
                     AVTGKVSGKLDAPGVLTHAKGSYVLQSPVAGQVVQVLAEEGRMLPANTPVLKVQTDRGY
                     RAVRTVSAGRMTSLTAKIGSVIAPGKDVATVERVERADEPLVAMLYVPGSSGSTVSPGA
                     AVDLTVQSAPTDEYGVLRGRVKAVGRAAQTRQQVTSFLGNPQLGAQFTKDGQPIAIMVT
                     LDKSATTKSGYRWSSEKGPPYKIDSMTIAEGAIHQPSERPIDWLLP"
     misc_feature    4696..4707
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.66"
                     /tool="antismash"
     gene            complement(5001..5204)
                     /locus_tag="G4Z16_RS19845"
                     /old_locus_tag="G4Z16_19820"
     CDS             complement(5001..5204)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412458.1"
                     /locus_tag="G4Z16_RS19845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_19820"
                     /product="hypothetical protein"
                     /protein_id="WP_028436646.1"
                     /sec_met_domain="strepbact (E-value: 1.9e-19, bitscore:
                     68.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFIETREISDNDLDNVSGGVLGSVVGTAVSTADSVAPVSETTDA
                     VNGVASGLTGVDTSQVTGLAGL"
     gene            6415..7713
                     /locus_tag="G4Z16_RS19850"
                     /old_locus_tag="G4Z16_19825"
     CDS             6415..7713
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="G4Z16_RS19850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_19825"
                     /product="MFS transporter"
                     /protein_id="WP_197352064.1"
                     /transl_table=11
                     /translation="MRATSPPAPPPSVRRARAAVSAVFFVHGAVFASWAARVPAVQDQL
                     QLSAGMLGLVMAGPGLGALAGSQAGGLLVHRLGSRAVSASAPVSLCVPLALVPAAPSEW
                     TLMAVLVLLGAADGCTSVAMNAQAVVVQGRYGRTVLNSMHAIRSIGAVAGGLAGVATVS
                     LGLSLAAQFAVMAAVLAVLSAVAACGLLPDGGPVEPAGEGAGERREGGAEEGRTHLAPA
                     PAAEERQGRPVKSSGARRRSATVLLLAVTTFLAALVEDAPASWSGVYLRHIGADAATAA
                     AGYAAFSAGSVVTRLLNDRLVNRLGWVRLIRTGTLCCAAVLAAALLLGTPAVALAAFVV
                     AGAGISAVFPGAFTAAGALRDPAPAMGQIGFAGNLGWLLVPPLIGGLATLVGLPAALGV
                     LVLASLAIAALAPVVRADTVRTTALNGVTGELR"
     gene            7819..8295
                     /locus_tag="G4Z16_RS19855"
                     /old_locus_tag="G4Z16_19830"
     CDS             7819..8295
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546554.1"
                     /locus_tag="G4Z16_RS19855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_19830"
                     /product="phospholipase"
                     /protein_id="WP_197352065.1"
                     /transl_table=11
                     /translation="MRRRLAAGVVVSSASLLAVIGTATTAVADPADKPQVLASWTQTDA
                     GSQNAFLAARADQGSWAAYNFDWSTDVCSSSPDNPFGFPFENGCIRHDFGYRNYKAQGT
                     FEANKSRVDSAFYEDLKRVCAGYSGATKESCNALAWTYYQAVVILGVKKGSPVG"
     gene            complement(8471..8704)
                     /locus_tag="G4Z16_RS19860"
                     /old_locus_tag="G4Z16_19835"
     CDS             complement(8471..8704)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187919.1"
                     /locus_tag="G4Z16_RS19860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_19835"
                     /product="hypothetical protein"
                     /protein_id="WP_197352066.1"
                     /transl_table=11
                     /translation="MAVCEVCGNDYEYTFEVRAAGAVHTFDSFECAAQKMAPICENCGC
                     RILGHGLQAEGRFFCCASCARMQGRMELADSV"
     gene            complement(8853..9272)
                     /locus_tag="G4Z16_RS19865"
                     /old_locus_tag="G4Z16_19840"
     CDS             complement(8853..9272)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077214.1"
                     /locus_tag="G4Z16_RS19865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_19840"
                     /product="CBS domain-containing protein"
                     /protein_id="WP_197352067.1"
                     /transl_table=11
                     /translation="MTTARELMSPGADCVQTDQTAADAARIMTEAGVGSLPICGQDDKI
                     KGVVTDRDIVVKVIGKGRDPQAFPAGSLNQEEAVTIGADDDTEDILATMTRHRVRRLPV
                     IDGTRLVGTVAVADVARSLPNATTGELVEALSADH"
     gene            9525..10127
                     /locus_tag="G4Z16_RS19870"
                     /old_locus_tag="G4Z16_19845"
     CDS             9525..10127
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="G4Z16_RS19870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="G4Z16_19845"
                     /product="hypothetical protein"
                     /protein_id="WP_197352068.1"
                     /transl_table=11
                     /translation="MREEMKWAFGLGLAAAIAASGAIDAAMQDDGASDTAAKAGSTARA
                     SAEPSPTPSSEPTATPSPTPTQTSAAADDGPDEICYPLATLKPLPRLTAVVDPRTGLKT
                     FKKTYEETEPPEPVCIPVHQPSYSGYPYSYTPPYSSQVLPTNAPGYAPGFGGAGSGSAT
                     GTEKDRRVPITTDRGGMPDFAPTVPRAPAPRVPAFIP"
ORIGIN
        1 ccctgcgcgg gagcggacag ctcgatgcct gccgcgccgg cggccttgcg ggcgacgacg
       61 agtgtcgcgt cgtcgttcgc ggacgaggag cctgaggagc ccttgccggt ggtgttgatg
      121 gaggccagca gcgcctggtc ggcctgggcg cgcacggtct cgccggcctc gatgcccgcg
      181 gccgtacggt cctcgtgcgc acgctcgagc cgttcgatcc accggtcgag ggcggagagc
      241 agccggaact gctggtcgac catgcgctgc cacactgcgc cgtcgacgag cagttgggac
      301 gccgcctcct ggctgaactc ggccccgtac tggacgctgc ggggatccac gtgcatccag
      361 aggatctcgt cgtcgtcctg gccgtaaccg gcgcccgggt ggacggcgtc cggggtgacc
      421 tcgccgtcga gcggcgcctc gaagagcacc cggaggctgc gtccgatgcc cgtggcgaag
      481 gcattgtcga gcggggtgtg gggcagtgcc gcgatgccgt cggccggggt ctgggtctgg
      541 tcccaggcag cgtggccctg gaagtgggcg gagacactgc tgtactcggg cctgtgcagt
      601 tcgcggagtt cgatgcggcg cagcccgcac tcccgcagcg gccttccgac cagcgtgtgc
      661 tgagtgccct cgatcgggcc gaggagcagg gttccgggct ccagcctgcc gaggaagtgc
      721 cagtggccct gctgaaccac gtcgaccgcg aacaggtcca tggctccggc ggtcaccagc
      781 cacagcacat gggggccttc gagggggagg cttcgcatgc cgacgcagtc gactccgtcg
      841 ccgagcatgc cgaaggcggc ggtcaccggg tcctgggggt gcgtcgcgac ggtgctgtgc
      901 ccgccggtgt cgtgggcggg ggagcggtgc tctcccgtgt cctgcatcgg gacccggtgc
      961 tcgccggtgt cgtaggcggg agccgggtgt tcggcggtgt cgtacgagtt cgtgccgtac
     1021 gggggagtgc cgtgcgcgcc tgtggcgtac gcgcctgtgt cgtacgtgcc cgtgtcgtac
     1081 gtgcccgtgt cgtacggagt gccgtacgag ccgccgccct ggcccacggc ctgttgcgta
     1141 ccggtgtcgt gcgtgccgtt ctgcggactc ggggtgccgt acggcggtgt gtgctcgctc
     1201 tcctcggccg tgccgaagga gcccgggtgc cactggcccg cctgccacga gccggtgtcc
     1261 tgcccggtcg tgcgcgggtc ccgtgcggag ccgtcgccgg atgccacgtc agtgctcctt
     1321 gaccagttcg gcgtacgggc cgccgcgggc gaccagttcc tcgtggcgcc cgcgttcgac
     1381 gatactgccg tggtccagca tgacgatctc gtcgctgtcg cggaccgtgc tcagcctgtg
     1441 agcgatgacg acgcacgcgc agccgcgccg ccgcaggttg tcgatgatga cctgctcggt
     1501 ctgggcgtcc agcgcgctcg tcacctcgtc cagcacgagg atgctcggcc ggcgcaccag
     1561 cgcgcgggcg atctcgaggc gctgcctctg cccgccggag aggttgcggc cgtcctgctc
     1621 gacgcggctg tggatgccgc cgggacggcg cgtgaagtcg tcgagcacgg cggcgtcccg
     1681 caaggcggcc gtgacggcct cgtcggaaat ggacgggtcc cacagcgcca cgttgtcccg
     1741 caccgttccc tcgaagagga agacgtcctg gtccacgaag gagacggagg cggccagcgc
     1801 gctgcgggag atgtcctcca gccgctgccc gtcgatgcgg atcactccct cccacgggct
     1861 gtagagaccc gaaatcagcc tggagacggt ggacttgccg ctgccggagc cgccgacgag
     1921 cgcgacctgc cggcccggtc cgaccgacag ggagaagtcc gtgagcagcg gcttgtcgag
     1981 cgggctgtag ccgaaggtga tgctctccag cgacacatgg cccttgaggc ggcgggtgtc
     2041 ggcgatgggc tcgcgccgcg aatagagcga gtcgacgggg aagttctcca cgtccttcag
     2101 cctggccacg tcggcggcga agtcctggat gcgtcccgcg acgccgttga gccgcgtgat
     2161 gggagcggtg aagcgcgtca cgagcgcctg gaaggcgacc agcagaccga tggagatgtg
     2221 cccctcgacc gcgcgcatgc cgccgatcca gaggatcatc gcgctgttga gcgtcgcgag
     2281 cgtgggcgcg acgacggcga gccaggccgt gggcacgccg agcttctgct gctcctccag
     2341 ggtcgtggcg tgctgcccgg accagcggcg gaagtacccg ttctcgccgc cggtggcctt
     2401 catcgtctcg atcaactgca ggccggtgta agaggtgttg gtgaggcgcg cggtgtccgc
     2461 gcggagcttc tgcgtgccgg tggcgcgcag ccggatcacc acgcgcatcg cgacgaggtt
     2521 caacagcgcg atgagcacac cgatgacggt cagctggggg tcgtacgtcc acagcagcac
     2581 cgcgtagagg atgacgacga tcgcgtccac gcccgcggcg gcgaggtcgc gggagagcgt
     2641 ctcggcgacg gtgtcgttgg actgcagtcg ctgcacgagg tcggcggggc tgcgctgcgc
     2701 gaagaacgtg accggcagcc ggagcagatg ccggaagaag cgtgcgctgc tgagcgtcga
     2761 ggagatgatg cggccgcgca gcagattcgc ctgctggagc gcggtcagcg cggcggtcag
     2821 cacgaccatg gcgcccatcg aggcgaacag cacgcccagc agcgacgtct ggtccccgat
     2881 gaggaacatg tcgatgtacg tgcggctcag cgccggcacc gcggcgccca ccccgacgag
     2941 caggaggctg gccagcagcg cggccagcat cgtgccggtg gtgcggcgaa gcctcgccgg
     3001 catggcgctc atggcactgg gacgcttgcc gccgcggcgg aagtcctcac cgggttcgaa
     3061 ggtgaggacg acgccggtga agctggtgtc gaactcgtcc agcgggacga aacgccggcc
     3121 cttgtcgggg tcgttgatgt gcacgccgcg cttgccgaag cggcgtccca tcccctcgta
     3181 gacgacgtag tggttgaact cccagaacag gacggcgggt ccgggcacct ccgcgagcgc
     3241 cgcgggctcc atctgcatgc ccttggcggt caggccgtaa ctgcgggccg ccttcaggag
     3301 gttgctggcg cgcgagccgt cgcgggagac gccgcaggcg atgcgcagct gttcgagggg
     3361 gacgtgccgg ccgaagtagc cgaggaccat ggacagggcg gcggcgccgc actccacggc
     3421 ctccatctgc agcacggtcg gtgtgcgtac cgacttgtac cggggcttcg gtatctgctg
     3481 cggcgacgta cgccgggcgg cgcccgtacg gtgcttgccg ctgctcttcg cgccggactt
     3541 gccgctcttg ccggtctcgg ggcggtgttt cctgcggccg gggggcggca gccggttctc
     3601 gccggtgtcg tccggctcgg gggcgcgtgc ctcctcggtg cgtgccttct tgggacgcgc
     3661 gtccttcttg gtgcgtgcct tccttggagg tgcgtccttg gtgcgtgcct tctgggaacg
     3721 cggcgcctcg gtgcgtgact tcgggaagcg cggtggttcg gaacgcggtg gttcggaacg
     3781 cggcggttcc gaacgcggtg gttccgaacg cggtggttcg gtacgtgcct tctggggacg
     3841 cgcggcctcc gaacgcgccg ctttcaggcg cgcggcgctc gtgcgcggct ccttggcggc
     3901 cttctgccgc cggctcacgg gagcagccaa tcgatggggc gctccgaggg ctgatgtatc
     3961 gcgccttcgg cgatcgtcat ggagtcgatc ttgtagggcg ggcccttctc cgaggaccat
     4021 ctgtaaccgg acttcgtcgt cgccgacttg tccagcgtca ccatgatcgc gatgggctgg
     4081 ccgtccttgg tgaactgcgc gccgagctgg gggttaccga ggaagctggt gacctgctgg
     4141 cgggtctggg cggccctgcc gacggccttg acgcggcccc gcaggacgcc gtactcgtcg
     4201 gtcggcgccg actggacggt caggtcgacc gcggctccgg gggagaccgt ggaaccgctg
     4261 ctgcccggta cgtacagcat ggccacgagc ggctcgtccg cccgctccac acgctcgacg
     4321 gttgccacgt ccttgccggg ggcgatcacg gagccgatct tcgcggtcag cgatgtcatg
     4381 cggcctgcgg acacggtacg aactgccctg tacccgcggt ccgtctgcac cttcagcacc
     4441 ggagtgttgg ccggcagcat gcggccctcc tcggccagca cctgcacgac ctggcccgcg
     4501 acggggctct gcagcacgta gctgcccttg gcgtgggtga ggacaccggg cgcgtccagc
     4561 tttccggaga ccttgccggt gacagcccaa tagcccgctc ccgccatgac gagaacggtc
     4621 acgagcagca cgagccagcc ctgcgggcgc gcgtagcgca caggcaggtc gagttcctcg
     4681 ggggactgca gcttggaaaa cgccttctgg cggaactgca cggggctatt ccctcaactg
     4741 cgttcgtcgc tttcaggcat gacgcttcct tcggggcggc gggcggtaca ccgggttcgt
     4801 cggtcgagcg gtccggcgct cggccgccga cgccaattcg gggacaaggg ccacgagtgt
     4861 tccacaactg tccccggacg catccgtaag ccccggaaca agggggttcc ggggctcacg
     4921 ttcaccccac tgggattcgg atgctccgag cgggacgggc tgtgcactgc tggcccggcc
     4981 gctcggtcgc gaccatcgcc tcagagaccg gcgaggcccg tgacctggga ggtgtcgaca
     5041 ccggtcaggc cggaggcaac gccgttcacg gcgtcggtgg tctcggaaac cggggcgacg
     5101 gagtcggcgg tgctcacagc ggtgccgacg accgagccca ggacgccacc cgaaacgttg
     5161 tcgaggtcgt tgtcggaaat ctcgcgggtc tcaatgaagt ccatgagttg agcttccctt
     5221 cgcttgcttt ggtgttctca atagcaacgg ccggctcggg gaaagccgtc cggccgtacg
     5281 agcggtcgtc tgatgcccga cgtcgggaac cattcgttca acttccgcgt cagggcccga
     5341 tcaaagcatg cgtacatgcc gcccggccac tcaaccgtgt gctcgcccgg cgccgatacg
     5401 gccactcaac ttcgttgcgg tgtagtcgcg tccacgaatt cgtggcgatc tcttcacttg
     5461 tcactcctgg cgtgtccgca cggggcggtg tctgacgcga cctcaaatgg acattccctc
     5521 atcaacctca ccggccccgg tgtggccggg tgcacatccc aggaggcatg tgccgtccct
     5581 aaggaccagt tgtgcaggtt cgctgaattc agtttcggtg ggcagcactt gctccggcct
     5641 gcgcaggtct gacctgggaa tggggcctct gtcgaggcga tccacggaag aggtgactcg
     5701 tggtaatgga agaaatccgc accggagtcc tcccgtaata cgagcgccta aggagtggta
     5761 agggcgaaat gactcgttca aatgctgaga catgcacgcg gcgtgcacat ccgaatcatg
     5821 gcccgacctc ggaagccgtt cctgccgtgg aatccataag tgtcctgcga tcgccgcaac
     5881 atgacggcaa gtcagccaac tgctccggtc cgtacggaaa tggggctttt cgacgcctgg
     5941 gaagaggtgg tcgcgtccgg tgggtgaatg cccggcacgc gccctcatgg aagtcccggg
     6001 gcgggcgggt ggacggccgc ggccgagagg agtgggcggg tgacggcacc ggcatctgcc
     6061 ggacctggcc cggccggtga cgggctgtgc ggtcctggcc cggccggtga cgggctgtgc
     6121 ggtcctggcc cgcgatcgcc ccggggtgaa ggtgaccggt cctatatccc gcgagtggga
     6181 caaaatccag gaaatcggag agccgtgcag aaccgtgacg aatccaggcc gccctcgttg
     6241 aatgtgtgca ggctggtcgt gggccttccc cacgttggcg atcgaggccc cgggctcctc
     6301 ccccggggcc tcggtctttc ctgaacggat ccgcccgcca agggtcgtcc ggagaatggc
     6361 cccgtgtgta ctggggaggg tcagccagtg ccgtcatgga agcggagccg cccggtgcga
     6421 gcgaccagtc ctccggcgcc gcccccgtcc gtgcgccggg cacgtgccgc cgtctcggcc
     6481 gtcttcttcg tgcacggcgc ggtgttcgcg tcgtgggccg cccgcgtgcc cgccgtccag
     6541 gaccaactcc agctgtcggc aggcatgttg ggtctcgtga tggccggtcc cggcctgggt
     6601 gccctggccg gcagtcaggc gggcgggctg ctggtgcacc ggctgggcag ccgcgcggtc
     6661 agcgcctcgg caccggtgtc gctgtgcgtg cccctggccc tggtcccggc cgcgccctcg
     6721 gagtggaccc tgatggcggt gctggtgctg ctgggtgccg ccgacgggtg cacatcagtg
     6781 gcgatgaacg cccaagcggt cgtcgtgcag ggccggtacg ggcgcacggt gctcaactcc
     6841 atgcatgcca tccgcagcat cggagccgtc gcgggagggc tggccggagt cgccaccgtc
     6901 tccctcggcc tgtcgctcgc cgcgcagttc gccgtcatgg ccgccgttct cgccgtgctc
     6961 tcggcggtgg cggcgtgcgg gctgctgccg gacgggggac cggtcgagcc tgcgggtgaa
     7021 ggggccggcg aacgccgcga gggcggcgcc gaagagggcc gcacgcacct ggccccggct
     7081 ccggccgccg aggaacggca ggggaggccg gtgaagtcct cgggcgcgcg gcgcaggtcg
     7141 gccaccgtgc tgctgctcgc cgtgacgacc ttcctcgccg cactcgtgga ggacgccccc
     7201 gcgtcctgga gcggtgtgta tctgcgtcac atcggtgccg acgcggccac ggccgccgcc
     7261 gggtacgccg ccttcagcgc gggcagcgtc gtgacgcggc tcctcaacga ccggctcgtg
     7321 aaccgcctcg ggtgggtgcg gctgatccgg accggcacgc tgtgctgcgc cgccgtactg
     7381 gccgcggccc tgctcctcgg gactcccgcg gtggcgctcg ccgcgttcgt ggtcgcgggc
     7441 gccgggatca gcgcggtctt ccccggtgcg ttcaccgccg cgggtgcgct tcgcgatcct
     7501 gcgccggcga tgggtcagat cgggttcgcc ggcaatctcg gctggctgct cgtcccgcct
     7561 ctgataggcg ggctggcgac gctcgtcggg cttccggccg ctctcggagt gctggtgctc
     7621 gcgtccctcg ccatcgccgc gctcgcaccg gtggtacgtg ctgacaccgt ccgtaccaca
     7681 gccctcaacg gcgttaccgg cgagttgcgt tgaacgcaaa acttctgcgc acgcgcctat
     7741 ttggcaagcg cttgttctgt catgctcatg cttcaacagt tccgatgttg ctgcacctct
     7801 cccccacgga ggtaagaaat gcgtcgaaga ctcgctgcgg gcgtcgttgt ttcgtccgca
     7861 agtctcctcg ccgtcatcgg caccgccaca accgctgtcg cggacccggc cgacaaaccc
     7921 caggtcctcg cgagctggac ccagaccgac gccgggagcc agaacgcctt cctggccgcc
     7981 agggccgacc agggctcatg ggccgcctac aacttcgact ggtcgacgga cgtctgctcc
     8041 tcgtcccctg acaacccgtt cggcttcccc ttcgagaacg gctgcatccg gcacgacttc
     8101 ggctaccgca actacaaggc tcagggcacc ttcgaggcca acaagtcccg cgtcgacagc
     8161 gccttctacg aagatctcaa gcgcgtctgc gccggataca gcggcgcgac gaaggagtcc
     8221 tgcaacgctc tcgcatggac ctactaccag gccgtcgtga tcctcggcgt caagaagggc
     8281 agcccggtcg gctgacgcac cgccggacgg ggcacagtga cgaagtaccg cgaagaggta
     8341 cctcgatgag gcacggcgca acagtgcgcg ggacgccgct cggtggctgc ggcgtcccgc
     8401 gcactctcgt catccggtca ctgcccgcac gtcccgcgcg gcgaccgtga gcatccctgt
     8461 gccgggcgga tcagacgctg tccgccagtt ccatgcgccc ctgcatgcgg gcgcagctgg
     8521 cgcagcagaa gaagcgcccc tcggcctgca ggccgtgccc gagaatgcgg cacccgcagt
     8581 tctcgcagat gggggccatc ttctgcgcgg cacactcgaa gctgtcgaag gtgtgcaccg
     8641 cgcctgccgc ccggacttcg aaggtgtact cgtagtcgtt cccgcagacc tcgcatacgg
     8701 ccatggcatg gctcctcggg tgatgtggat ggcttgacgg tgtggagaag cggacggacc
     8761 gcttcgaagc ggaggccgtc ggccggtgtg cggccgtgcg gcctccgcgc ctgtgcgggt
     8821 cctgtgagca gtggtcggtg gtgccggtgt cgtcagtggt cggcgctgag cgcctccacc
     8881 agttcaccgg tcgtggcgtt cggcagcgac cgggccacgt cggcgacggc caccgtgccg
     8941 acgagacggg tcccgtcgat caccggaagt cggcgcaccc tgtggcgcgt catcgtggcc
     9001 aggatgtcct cggtgtcgtc gtccgcgccg atcgtgaccg cctcttcctg gttgaggctg
     9061 cccgccggga acgcctgcgg gtcgcggccc ttgccgatca ccttcacgac gatgtcccgg
     9121 tccgtgacaa cacccttgat cttgtcgtcc tggccgcaga tgggcaggga accgacaccg
     9181 gcctcggtca ttattcgcgc ggcgtcggcg gccgtctgat ccgtctggac gcagtcggcg
     9241 ccggggctca tcagttctcg tgccgtagtc atgtgaaact cctggacgac tgaattcgtg
     9301 cggcggcgcg ggtccctcga gtccggccgt caatggccgg aggaaccgcg ccgtcgccga
     9361 tcgggtagca cgtccccgcg gactcaaacc ccggcgtacg gggggcgcgt ggcagccggg
     9421 agccgcccgt cgccccgcgg aaggtggccg gaacgggacg gaaacgcgtg ttgccggtgc
     9481 gtcgcgtccc ataccgtggt tcaggcatac gggggtgagc gcgaatgcgc gaggagatga
     9541 agtgggcctt cgggctcggg ctggcggccg cgatcgcggc cagtggggcc atcgacgcgg
     9601 cgatgcagga cgacggggcg tcagacacgg cggcgaaggc cggttccacg gcacgagcct
     9661 ccgcggagcc gtcacccacg cccagttcgg agccgaccgc cactccgtcc cccacgccca
     9721 cgcagacctc ggccgccgct gacgacgggc cggacgagat ctgctacccg ctcgcgaccc
     9781 tcaagcccct gcccaggctc accgccgtgg tggacccgcg gacagggctg aagaccttca
     9841 agaagacgta cgaggagacg gagccgcccg agcccgtctg catccccgtc catcagccct
     9901 cgtacagcgg ctatccgtac tcgtacacgc ccccctactc ctcgcaggtc ctgccgacga
     9961 acgcacccgg ctacgcccct gggttcggcg gcgcggggtc cggttccgca accggcacgg
    10021 agaaggacag gcgggtcccc atcacgacgg accgcggagg gatgcccgac ttcgccccca
    10081 ccgtgccgcg ggcgcccgcg ccgcgtgtgc ccgcgttcat cccctaggcg ggccccggcg
    10141 cagcgccggc atccccaccc ccatccccat tcacattccg attcccatcc acatttgctt
    10201 ccgc
//
