LOCUS       NZ_CP048882            11315 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Streptomyces bathyalis strain ASO4wet chromosome, complete genome.
ACCESSION   NZ_CP048882
VERSION     NZ_CP048882.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces bathyalis
  ORGANISM  Streptomyces bathyalis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7377472)
  AUTHORS   Risdian,C., Landwehr,W., Rohde,M., Schumann,P., Hahnke,R.L.,
            Sproer,C., Bunk,B., Kampfer,P., Schupp,P.J. and Wink,J.
  TITLE     Streptomyces bathyalis sp. nov., an actinobacterium isolated from
            the sponge in a deep sea
  JOURNAL   Antonie Van Leeuwenhoek 114 (4), 425-435 (2021)
   PUBMED   33595745
REFERENCE   2  (bases 1 to 7377472)
  AUTHORS   Risdian,C., Landwehr,W., Schupp,P. and Wink,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2020) Microbial Strain Collection, Helmholtz
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig,
            Niedersachsen 38124, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-FEB-2019
            Assembly Method                   :: HGAP v. v3
            Assembly Name :: Streptomyces_DSM106605_HGAP3_1SC_10M
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117.0x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910445.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 04:35:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,467
            CDSs (total)                      :: 6,387
            Genes (coding)                    :: 6,276
            CDSs (with protein)               :: 6,276
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 37 of 111
            Pseudo Genes (incomplete)         :: 90 of 111
            Pseudo Genes (internal stop)      :: 12 of 111
            Pseudo Genes (multiple problems)  :: 25 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:44:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1323285
            Orig. end                         :: 1334600
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from MISG, Helmholtz Centre for
            Infection Research, Germany.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11315
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11315
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11315
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6315](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6315
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(157..2364)
                     /locus_tag="G4Z16_RS05835"
                     /old_locus_tag="G4Z16_05830"
     CDS             complement(157..2364)
                     /GO_function="GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1293: aldehyde oxidase and xanthine dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562341.1"
                     /locus_tag="G4Z16_RS05835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_05830"
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="WP_197349530.1"
                     /transl_table=11
                     /translation="MTAAEAEPVVGPGIDRVDGARKVEGAAAYPSDVQVPGMVHAVLVQ
                     STVAAGRIRTLDTGAAEASQGVLAVITHRNAPRLPGGPMTALGATPPAPLQDDRILHHG
                     QHVAVVVAETHEQATAAARMVRVVYEVTDAVLRLDDPRAERLQNPWGLDTDRGATGAAL
                     EAAEVKLSGTYTTADNTNNPLGCFATVAVWERNGLTVHDATQWPTMVRSTLARTFDLPE
                     SAVRVLAPYVGGAFGAGLRAWPHVVLAVLAARMLGRPVKLVLTRPQMFTSIGHRPRGVQ
                     HLSIGSSRDGELVALDHRSTSTVAMEDEDFEPVAALSALSYACPNVITRDSQARLNIPD
                     PTSMRAPAEAQGNFAIESALDELAHALEMDPIELRLRNYAETHPGNGMRWSSKALRECY
                     EAGAELFDWSRRTPEPRSMRDGDWLVGYGMAGVSYPWYAQPCNARATVNRDGSALVRSA
                     ATDIGTGTYTVMTQVSADALGIPVGRVRFDLGDSDMPQAPQAGGSGLTGALAPAVQDAC
                     RGVLQRVLDLVRDDADSPLRGCTLEDVTVRGGRIHRRDRPGEGESYADILERHGHQELS
                     ADGASTPADPSVLGMAPAGAFGAKFVEVRVDAELGIIRVPRVVSAIDGGRILNEKTARS
                     QIIGGTVGGIGQALFEETITDDDTGRIANATFGDYLVPVNADVPQVDVVFVGAPDPATP
                     LGTKGIGEIGLVGIAAAVANAVHHATGTRIRSLPITLDRLL"
     gene            complement(2361..3350)
                     /locus_tag="G4Z16_RS05840"
                     /old_locus_tag="G4Z16_05835"
     CDS             complement(2361..3350)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562342.1"
                     /locus_tag="G4Z16_RS05840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_05835"
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="WP_197349532.1"
                     /transl_table=11
                     /translation="MLPFEYARAADVDEAVNLLAGDPSASYLAGGTTQLDLMKDGVLGP
                     ERLVDITRLPLRGVTRDGDGLHIGALTTMEELAADPTVTDELRIVRQALLLGASTQLRN
                     MATIGGNLLQRARCRYFRDPSVPDCNKREPGTGCAAVNNTARMHAILGVSERCIAVHAS
                     DLAVALVALDAVVHLKGTSGGRSVPLTQFYREAGSSPDVENVLVHGELITAITIPFLPP
                     TAVSGYLKVRDRASYEFALASAGVALVVQDGTINQARVGLGGVGSKPWRSLEAEQVLTG
                     SPAGEETFREAAAAAVQGAWTVSGTAFKVELAQRTLVRTLRTVSGVTS"
     gene            complement(3337..3864)
                     /locus_tag="G4Z16_RS05845"
                     /old_locus_tag="G4Z16_05840"
     CDS             complement(3337..3864)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004943248.1"
                     /locus_tag="G4Z16_RS05845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_05840"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_197349534.1"
                     /transl_table=11
                     /translation="MAKALSQRREARSVPDAPVELHVNGRRHKLHLDVRVTLLDALRDR
                     LGLTGTKKGCDQGTCGACTVHVEGRRVLSCLTLAAQCDEREVTTIEGLTAPDGGSHPLK
                     ESFVRHDAFQCGYCTPGQIMSAAALLEEGRAGSDEDIQEFMSGNLCRCGAYVNIVAAVR
                     DVASTGGADASL"
     gene            4197..4862
                     /locus_tag="G4Z16_RS05850"
                     /old_locus_tag="G4Z16_05845"
     CDS             4197..4862
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130509.1"
                     /locus_tag="G4Z16_RS05850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_05845"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_343070695.1"
                     /transl_table=11
                     /translation="MNDAQEYALPLVVRVERAAPPERTDAMETAARAVLTLLSDERSAK
                     DGEWAERVRLWEDSAIRKVVRRARGAEWRRAEALPGVTVTGRTAAVRVYPPVPVDDWPG
                     ELARLQVSGTELTDPEPPPAPPHGVPVLWLAPDLEMSAGKAMAQAGHSAQLAWWQLSGV
                     AREEWREADFALAVRTAGGPGQWAGLVRSGLPVVRDAGFTEVAPGSVTVVADHPALRT"
     gene            5001..6315
                     /locus_tag="G4Z16_RS05855"
                     /old_locus_tag="G4Z16_05850"
                     /pseudo=""
     CDS             5001..6315
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643869.1"
                     /locus_tag="G4Z16_RS05855"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="G4Z16_05850"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 7.3e-94, bitscore: 312.5,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGTARTRTAGTAVRLGTGIGWRPEIADGIEALPGIDWVEVVAENI
                     CPDHLPDALERLRERGTTVVPHGVSLGLGGAEPPDPARLSALAECAEALGAPLVTEHIA
                     FVRAGGAPTGLPRMEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALVEWP
                     GEELTEGAFLAELVERTGVRLLIDVANLHTNHVNRGEEPSVALDELPLDALAYVHVAGG
                     FERDGVWHDSHGHPVPQSVLDVLAQLCARTEPPGVLLERDENFPPVSELAGELDSIRGM
                     VEQGVRARELSTHGD"
     gene            6612..8192
                     /locus_tag="G4Z16_RS05860"
                     /old_locus_tag="G4Z16_05855"
     CDS             6612..8192
                     /NRPS_PKS="Domain: Abhydrolase_1 (107-477). E-value:
                     8.8e-16. Score: 50.2. Matches aSDomain:
                     nrpspksdomains_G4Z16_RS05860_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888542.1"
                     /locus_tag="G4Z16_RS05860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_05855"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_197349535.1"
                     /sec_met_domain="PF00561 (E-value: 7.7e-21, bitscore: 73.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRSAAIHATLGALALTAAAVLPGDITPGGLPLPLRDAREAVGAAK
                     AAEAAEAAGIGWSRCPAAEELPASTECGSVTIPVDYADPDGEKFRLAVSRKRATGPAAL
                     RRGPLLHNPGGPGADGMDFPLHATVPGGVWKKLNRSYDLVGYAPRGVGRSGPLLCADPD
                     EADSGASRSPRVPSEAFKRQMRRQAAAFARACAREHGARLDHFTTADNARDLDVLRAAL
                     HRERTDFIGTSYGSYLGAVYATLFPERVGRLVLDSVVDPRPDRVWYRNGLEQNKAFQRR
                     WGDFMSWVAEHDSAYGLGRTAHTVQRNFDRARDALDHGSVGGWAGDGVGSRQLLRGYLD
                     AGYSDESWPRLARALADFREGDPRALAELAAPGRRASAQRMNSDSVYRAVQCSEGHWPR
                     DWSRWDRDNTALAREAPFQTWENLRANLPCAYWQGRSVRPVDVGATPGELPPVLLLAST
                     RDAATPYEGAVETWKRLPGSSLVTERGSGTHGVAGGSACADRHLAAYLLDGRTPGRAAE
                     CPAPPGPRP"
     aSDomain        6933..8042
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_G4Z16_RS05860_Abhydrolase_1.1"
                     /evalue="8.80E-16"
                     /label="G4Z16_RS05860_Abhydrolase_1.1"
                     /locus_tag="G4Z16_RS05860"
                     /protein_end="477"
                     /protein_start="107"
                     /score="50.2"
                     /tool="antismash"
                     /translation="PLLHNPGGPGADGMDFPLHATVPGGVWKKLNRSYDLVGYAPRGVG
                     RSGPLLCADPDEADSGASRSPRVPSEAFKRQMRRQAAAFARACAREHGARLDHFTTADN
                     ARDLDVLRAALHRERTDFIGTSYGSYLGAVYATLFPERVGRLVLDSVVDPRPDRVWYRN
                     GLEQNKAFQRRWGDFMSWVAEHDSAYGLGRTAHTVQRNFDRARDALDHGSVGGWAGDGV
                     GSRQLLRGYLDAGYSDESWPRLARALADFREGDPRALAELAAPGRRASAQRMNSDSVYR
                     AVQCSEGHWPRDWSRWDRDNTALAREAPFQTWENLRANLPCAYWQGRSVRPVDVGATPG
                     ELPPVLLLASTRDAATPYEGAVETWKRLPG"
     gene            complement(8231..8671)
                     /locus_tag="G4Z16_RS05865"
                     /old_locus_tag="G4Z16_05860"
     CDS             complement(8231..8671)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088692.1"
                     /locus_tag="G4Z16_RS05865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_05860"
                     /product="DUF6010 family protein"
                     /protein_id="WP_197349537.1"
                     /transl_table=11
                     /translation="MNYVWPILVGVVYCLLMCLIREPHRRRFNAIMVGGAGAAYLSGGG
                     FGAWELPVVAVMTYVAYRGLESWTFIGIGWLIHTAWDLAHHLKGNPILPFQHDSSLGCA
                     ICDPVIALWCFRGGPSLITFVRGRLRPRRTADQPPHDAAAPC"
     gene            complement(8668..8823)
                     /locus_tag="G4Z16_RS05870"
                     /old_locus_tag="G4Z16_05865"
     CDS             complement(8668..8823)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="G4Z16_RS05870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="G4Z16_05865"
                     /product="hypothetical protein"
                     /protein_id="WP_197349538.1"
                     /transl_table=11
                     /translation="MHIQVVLLDGSDRLDVTAPGEVPHAPRGGQRHRAGAPVGTVRPEL
                     EEVIPS"
     misc_feature    8813..8826
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.25"
                     /tool="antismash"
     gene            9020..9958
                     /locus_tag="G4Z16_RS05875"
                     /old_locus_tag="G4Z16_05870"
     CDS             9020..9958
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1260: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384835.1"
                     /locus_tag="G4Z16_RS05875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_05870"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_197349539.1"
                     /transl_table=11
                     /translation="MHTVAVIALDQVIPFDLSTPVEVFTRTRLPDGRPGYQIRVCAEDE
                     EIDAGTFTLRAPWGLEGLQDADTIIVPGVADPAAPLSPPVRDALRSAAADGTRIASICT
                     GTFPLAATGLLDGLRATTHWLAAGLLAASHPDIDVDPDVLYVDSGQFLTSAGAAAGMDL
                     CLHMIRRDYGSAVAADAARLSVMPLEREGGQAQFIVHDHAPTPQGSDLETLLTWLRSNL
                     GRDLTLADIAAQSGTSTRTLIRRFREQTGTTPLQWLHRARIRQAQHLLETTEHSVERIG
                     TQVGFGSATTFRDRFKRTTGVSPKTYRRTFS"
     gene            complement(10086..10817)
                     /gene="hemQ"
                     /locus_tag="G4Z16_RS05880"
                     /old_locus_tag="G4Z16_05875"
     CDS             complement(10086..10817)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984746.1"
                     /locus_tag="G4Z16_RS05880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_05875"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_197349540.1"
                     /transl_table=11
                     /translation="MTAAPEPGSPEKTPNAGKKAKDLNEVVRYALWSVFRLRDVLPEDR
                     AGYADEVEELFGQLAAKDVVVRGTYDVSGLRADADLMIWWHAESSDALQDAYNRFRRTR
                     LGRALEPVWSNMALHRPAEFNKSHLPAFVAGEEPRAYVSVYPFVRSYDWYLLPDEDRRR
                     MLADHGKMARGFPDVRANTVPAFSLGDYEWILAFEADELHRIVDLMRHLRGSEARMHVR
                     EEVPFYTGRRKPVAELVAGLV"
ORIGIN
        1 gcccggcacg acgttcgccg tgactggtcc ctggaagcac tgacccgtcg tcgcaccacg
       61 cctcgcagga agggcccggt gtccccgtag ggacgccggg cccttcctgg ctctcagcgg
      121 tggccggtca cagcaggacg gtcaccgcag ggcaggtcac agcagccggt ccagggtgat
      181 cggcagcgac cggatgcgcg ttcccgtggc gtggtggacg gcgttcgcga cggccgcggc
      241 gatgccgacc agcccgatct ccccgatccc cttggtgccg aggggagtcg cagggtcggg
      301 cgctcccacg aagacgacgt cgacctgagg aacgtccgcg ttcacgggga cgaggtagtc
      361 cccgaacgtg gcgttggcga tgcgtccggt gtcgtcgtcc gtgatggtct cctcgaacag
      421 tgcctgcccg atgccgccga cggtcccgcc gatgatctgg ctgcgggccg tcttctcgtt
      481 gaggatccgg ccgccgtcga tcgccgagac cacgcgcggc acccggatga tgccgagttc
      541 ggcgtcgaca cgcacctcga cgaacttggc gccgaacgcc cccgccggag ccatgcccag
      601 cacggaggga tccgccggag tgctggcacc gtccgccgac agctcctgat ggccgtgccg
      661 ctcgaggatg tcggcgtagg actccccctc tccgggccgg tcccgccggt ggatgcggcc
      721 gcccctcacg gtgacgtcct cgagcgtgca cccgcgcagc ggtgaatcgg cgtcgtcccg
      781 caccaggtcg aggacccgct gcagcactcc gcggcacgcg tcctgcacgg caggcgccag
      841 ggcaccggtg agtcccgacc cgccggcctg cggggcctgc ggcatgtcgg agtccccgag
      901 gtcgaagcgg accctgccca ccgggatgcc cagggcgtcg gcggacacct gtgtcatcac
      961 cgtgtacgtg ccggtcccga tgtccgtcgc ggcgctgcgc accagcgcgc tgccgtcccg
     1021 gttgacggtc gcccgcgcat tgcagggctg cgcgtaccag gggtagctga cgccggccat
     1081 gccgtacccg acgagccagt cgccgtcgcg catggaacgc ggctcgggcg tacgccgtga
     1141 ccagtcgaac agctcggccc cggcctcgta gcactcccgg agcgccttgc tcgaccagcg
     1201 catcccgttc cccggatggg tctcggcgta gttgcgcagc cggagttcga tcgggtccat
     1261 ctccagggcg tgggcgagtt cgtcgagcgc cgattcgatg gcgaagttcc cctgcgcctc
     1321 cgcgggcgcg cgcatggacg tggggtcggg gatgttcaga cgtgcctggc tgtcccgggt
     1381 gatcacgttg gggcaggcgt aggagagggc cgacagcgcg gcgaccggct cgaagtcctc
     1441 gtcctccatg gccacggtgg aggtggaacg gtggtcgagg gcgaccagtt cgccgtcccg
     1501 gctgctgccg atcgacagat gctgaacccc gcggggacgg tgcccgatcg aggtgaacat
     1561 ctgaggccgg gtcagcacca gcttcaccgg ccgcccgagc atccgcgccg cgagcaccgc
     1621 cagcacgacg tgcggccacg cccgcagccc ggccccgaac gcgccgccga cgtacggtgc
     1681 cagcactctc acggcgctct cgggcaggtc gaaggtcctc gcgagggtgg agcgcaccat
     1741 cgtcggccac tgcgtggcgt cgtgaacggt caggccgttc cgctcccaga cggccaccgt
     1801 cgcgaagcag cccagtgggt tgttggtgtt gtccgccgtc gtgtaggtgc ccgacagctt
     1861 cacctccgcc gcctcgagcg cggccccggt ggcgccgcgg tcggtgtcga gcccccacgg
     1921 gttctgcagc cgttcggcac gcggatcgtc gagcctgaga acggcgtcgg tcacctcgta
     1981 gacgacgcgc accatccgcg cggcggcggt ggcctgttcg tgcgtttcgg cgaccacgac
     2041 ggcgacgtgc tgaccgtggt ggagaatccg gtcgtcctgg agcggtgccg ggggcgtcgc
     2101 gccgagcgcg gtcatcggcc cgcctggcag ccggggcgcg ttgcggtggg tgatgacggc
     2161 caggacgcct tgcgacgcct ccgccgcgcc cgtgtcgagc gtgcggatgc gcccggcggc
     2221 gacggtgctc tggacgagga cggcgtgcac catccccggt acctggacgt cgctcgggta
     2281 ggccgcggca ccctccacct tgcgggcacc gtcgacgcgg tcgattcccg gcccgacgac
     2341 gggttcggct tccgcggcgg tcacgaggtc acccccgaca cggtccgcag ggtccgcacc
     2401 agggtgcgct gggcgagctc gaccttgaag gccgtgccgg agaccgtcca ggctccctgt
     2461 acggcagcgg ccgcggcttc acggaacgtc tcctcgcccg caggcgaacc ggtgagcacc
     2521 tgctcggcct caagcgaccg ccacggcttc gaaccgacac cgccgagccc gacacgtgcc
     2581 tggttgatcg tgccgtcctg cacgacgagc gcgaccccgg cagacgcgag ggcgaactcg
     2641 taggaggcgc ggtcgcggac cttgaggtag ccggagaccg ccgtgggcgg caggaacggg
     2701 atggtgatcg cggtgatcaa ctcaccgtgc acgagcacgt tctcgacgtc ggggctgctg
     2761 ccggcctccc ggtagaactg tgtgagaggc acgctgcggc cgccggacgt ccccttcaga
     2821 tgcaccacgg cgtcgagggc gaccagggcg acggcgagat cggacgcgtg caccgcgatg
     2881 caccgctcgc tgacgcccag gatcgcgtgc atcctcgcgg tgttgttcac ggccgcgcac
     2941 ccggtgccgg gctcgcgctt gttgcagtcg ggcacgctcg ggtcgcggaa gtagcggcac
     3001 cgcgcccgct gcaggaggtt gccgccgatc gtcgccatgt tgcgcagctg ggtcgatgct
     3061 ccgaggagca gcgcctggcg gacgatgcgc agctcgtcgg tcaccgtcgg gtcggctgcc
     3121 agctcttcca tcgtggtgag ggcgccgatg tgcaggccgt ccccgtcgcg ggtcacgccg
     3181 cggagcggga gccgggtgat gtcgaccagc cgctcggggc cgagcacccc gtccttcatc
     3241 agatcgagct gggtcgtacc cccggccagg taggacgcgg aggggtcccc ggccaggagg
     3301 ttcacggcct cgtcgacgtc tgccgcgcgc gcgtactcaa agggaagcat cggcaccccc
     3361 ggtcgatgcg acgtcgcgca cggcggcgac gatgttgacg taggcgccgc agcggcagag
     3421 gttgccgctc atgaactcct ggatgtcctc gtcggaaccg gctcgtccct cctccagcag
     3481 ggccgccgcc gacatgatct gaccgggcgt gcagtagccg cactggaagg cgtcgtgacg
     3541 tacgaaggac tccttgagcg ggtgcgatcc cccgtcgggt gcggtcaggc cctcgatggt
     3601 ggtgacctcc cgctcgtcgc actgggcggc gagcgtcagg cacgacagga cgcggcggcc
     3661 ctccacgtgc acggtgcacg ccccgcaagt tccctggtcg cagcccttct tcgtccccgt
     3721 cagaccgagc cggtcccgga gcgcgtcgag cagggtcacc cgcacgtcga ggtgcagttt
     3781 gtgccgccgg ccgttgacgt gcagctccac cggggcatcg ggcacggagc gtgcctcgcg
     3841 tcgctgactc agtgctttgg ccatcgcctc tcctcagata tgcgggaacg cgggagaccg
     3901 gcactcgccg gcgagcatgg cgaggggctg gtcgctcccc tcgcgcacgg tcggtacgag
     3961 cggctactcg ctaacggaga accggatgtc atcaaaccgg cattcagcat aattcgggtt
     4021 gatcacacag agaggtgtac gcccgcccgg cgcggctccg gctccgctcc cccggtacgc
     4081 ccgggatccg gacggctttc acccgggctc tgccgtgacg agcagcgggg ccgggcggcc
     4141 gactccgtcc cctcccgcac gagttgaccg tttccgtcgg ctggcagact gaccgcgtga
     4201 acgacgcaca ggagtacgcg ctgccgctgg tcgtccgggt ggagcgggcc gcgcccccgg
     4261 agcgcaccga cgcgatggag acggcggccc gcgcggtgct caccctcctc tccgacgaac
     4321 ggtcggcgaa ggacggcgag tgggccgagc gcgtgcggct gtgggaggac agcgcgatcc
     4381 gcaaggtggt tcggcgggcg cgaggcgcgg agtggcggcg cgcggaagct ctgcccggcg
     4441 tgaccgttac ggggcggacg gcggcggtca gggtctatcc gcccgtgccc gtcgacgact
     4501 ggccggggga actggcgcgg ttgcaggtct ccggcaccga actcaccgac cccgagccgc
     4561 ccccggcccc gcctcacgga gtaccggtgc tgtggctggc cccggatctg gagatgtcgg
     4621 cgggcaaggc catggcacag gccgggcaca gcgcgcagtt ggcctggtgg cagctgtccg
     4681 gcgtggcacg cgaggagtgg cgcgaggcgg acttcgccct cgccgtgcgt acggccggcg
     4741 ggccggggca gtgggccggt ctggtccgca gcgggctccc ggtcgtacgg gatgcgggct
     4801 tcaccgaggt cgcgccgggt tccgtgacgg tggtcgcgga ccatccggct ctgcgaacct
     4861 gactgatccg ggtcgcggcc gccatccgta gcggtcgctt ccggtcgcac gaacctcaat
     4921 tcctcctctg aaagcgggcc ggggtggatt cgcccgcccg ttccgtggca ggagcgagga
     4981 agacgcaggg ggaggctcac atggggacgg cgcggacacg gacggcgggc accgcggtgc
     5041 ggctgggcac cggcatcggc tggcggcccg agatcgcgga cgggatcgag gctctgccgg
     5101 gcatcgactg ggtcgaggtg gtggcggaga acatctgccc ggaccatctg ccggacgccc
     5161 tggagcggct gcgggagcgc ggcacgactg tcgtcccgca cggcgtctcc ctcgggctcg
     5221 gcggagccga accgcccgac ccggcgcggc tgtcggcgct cgccgagtgc gccgaggcgc
     5281 tcggggcgcc gctcgtcacc gagcacatcg cattcgtacg ggctggaggc gcgccgaccg
     5341 ggctccctcg gatggaggcg ggccatctgc tgccggtgcc gcgcacccgg gacgccctcg
     5401 acgtgctgtg cgagaacgtg cggatcgcgc aggacgcgct gcccgtgccg ctcgcgctgg
     5461 agaacatcgc ggcgctcgtc gagtggcccg gcgaggagct gacggagggc gccttcctgg
     5521 ccgagctcgt ggagcgcacc ggtgtgcgcc tgctgatcga cgtcgccaat ctgcacacca
     5581 atcacgtcaa ccgcggcgag gaaccttcgg tcgcgctgga cgaactcccc ctggacgcac
     5641 tggcctacgt gcatgtggcg ggcggcttcg agcgggacgg ggtctggcac gacagccacg
     5701 ggcatcccgt gccgcagtcc gtgctggacg ttctcgccca gctgtgcgca cgtacggagc
     5761 caccgggcgt gctgctggag cgggacgaga acttcccgcc cgtgagcgaa ctcgcgggcg
     5821 agctcgacag catccgcggc atggtcgaac agggcgtgcg cgcaagggag ttgagcaccc
     5881 atggcgactg aggacactgc cggaacggcc ggcgcacggg aacgcctcgc acaggcccag
     5941 gccgggctgc ttgccgcgct cgtcgcgggc gcgcctgcac ccgagggctt cgaccacgag
     6001 cggctggccg tgcagagtcg cgcgctcatc ggaaagcgcg ccggcgtcgt cgagaaggtg
     6061 gcaccggaac tgccggagat catgggcgag tcggccttcc gcacggcctt cgcctcgtac
     6121 gcgcgggcga ggcccatgac cgggggctac cggcgcgacg cgctgcactt cgcggcgcac
     6181 gtactggacg agggcctcgc cggcgaccgc cgcgcacgca ggaagctgcg gtcgtggtgg
     6241 ctggagcgtt cgggcccgga gcccctgccc gaggggacgc tggcccgcac gctgcatctg
     6301 atgcgcgccc gctgacgaga gccgggcccg gcccgccccg cggccgttgc ggtcagtcac
     6361 ggtgatcgcc ggataacgac cactgatcgt ccccgaactc ccttgcgtac gggcggattt
     6421 cgtacggccg cccccgcggt cgccgcttcg ggcgcatccc cgccgacacg taccgccggt
     6481 tctttacttc gccaactccc gcacccagca tgcccgttgt cacacaccgc tcatgggggc
     6541 tcacacacag cacgcggcga acgcatgtct ccggcgcccc gccacagcac caacaggaac
     6601 gaggcaccac cgtgcgctca gccgcgatcc acgccacgct cggtgccctg gccctcacgg
     6661 ccgccgccgt cctcccgggc gacatcaccc ccggcggcct gccccttccg ctccgcgacg
     6721 cgcgtgaggc ggtcggtgcg gcgaaggcgg ccgaagcggc ggaggccgcc ggcatcggct
     6781 ggtccaggtg ccccgcggcc gaggagcttc cggcgtccac cgagtgcggc agcgtcacca
     6841 tccccgtgga ctacgccgat ccggacgggg agaagttccg gctggccgtc agccgcaaac
     6901 gtgcgaccgg tccggcggcc ctgagacgcg gccccttgct gcacaacccc ggcgggccgg
     6961 gcgccgacgg catggacttc ccgctccacg ccacggtccc gggcggcgtg tggaagaagc
     7021 tcaaccgctc ctacgacctg gtcggttacg cgccgcgcgg cgtcggacgc tccggcccgc
     7081 tgctgtgcgc ggacccggac gaggcggaca gcggcgcgtc gcgctcgccc cgtgtgccgt
     7141 ccgaggcgtt caagaggcag atgcgacggc aggccgccgc gttcgccagg gcatgcgctc
     7201 gcgaacacgg tgcgcggctc gaccacttca ccaccgccga caacgcccgc gacctggatg
     7261 tcctgcgcgc cgccctgcac cgggagagga ccgacttcat cggcacctcg tacggcagct
     7321 acctcggcgc cgtgtacgcc acgctcttcc cggagcgcgt gggccgcctc gtcctcgaca
     7381 gcgtcgtcga tccgcgcccg gatagggtct ggtaccgcaa cggcctcgaa cagaacaagg
     7441 cgttccagcg ccgctggggg gacttcatgt cctgggtcgc cgagcacgac tccgcctacg
     7501 gactcggccg cacggcgcac acggtccagc ggaacttcga ccgggcacgc gacgccctcg
     7561 accacgggag cgtgggcggc tgggcgggcg acggcgtcgg ctcacggcag ctgctgcgcg
     7621 gctacctcga cgccggttac agcgacgaga gctggccgcg cctggcccgc gccctcgcgg
     7681 acttccgcga gggcgacccg cgtgcgctgg ccgaactggc cgctcccggc cggcgcgcct
     7741 cggcgcagcg gatgaacagc gactccgtct accgcgccgt ccagtgttcc gaggggcact
     7801 ggccgcggga ctggtcgcgc tgggaccgcg acaacaccgc gctcgcgcgg gaggcgccgt
     7861 tccagacctg ggagaacctg cgggcgaacc ttccgtgcgc ctactggcag ggcaggtccg
     7921 tccggcccgt cgatgtgggc gccacgcccg gcgagttgcc gccggtgctg ctgctcgcgt
     7981 cgacccgtga cgccgcgacc ccatacgagg gcgccgtgga gacctggaag cggctgcccg
     8041 gctcctccct cgtcaccgaa cggggctcgg gcacgcacgg cgtcgccggc ggcagcgcat
     8101 gcgccgaccg gcatctcgcg gcctatctgc tggacggcag gacgccgggg cgcgcggccg
     8161 aatgccccgc gcctcccgga ccgcgcccct gacccctgcc ggtgacgacc cgggattccg
     8221 acgaagtccg ctagcagggt gcagccgcat cgtgcggcgg ttggtccgcc gtccggcggg
     8281 gcctcaaccg gccgcggacg aaggtgatca gcgaggggcc tccgcggaag caccacaggg
     8341 cgatgacggg atcgcagatg gcgcagccca gggaggagtc gtgctggaag ggcaggatcg
     8401 ggttgccctt gaggtggtgg gccaggtccc acgccgtgtg tatcagccag ccgatgccga
     8461 tgaaggtcca ggattccagg ccccggtagg cgacgtaggt catgacggcg acgaccggga
     8521 gctcccaggc gccgaagccg cctccgctca gataggccgc gccggctccc cccaccatga
     8581 tcgcgttgaa gcggcgtcgg tgcggttccc tgatgaggca catcagcagg cagtagacga
     8641 caccgaccag gatcggccac acgtagttca tgacgggatg acctcttcca actcgggccg
     8701 caccgtcccg acgggggcgc cggcccggtg ccgctgccca cctcgcgggg cgtgcggcac
     8761 ctcgccgggc gcggtgacat cgagtcggtc ggagccgtcg agcagaacaa cctggatgtg
     8821 catgaaggtc cttcggatgt cgatctctgt cggacgctcc gaagtcaacg cgctgccgcc
     8881 acattcgccc agtggcacga gtgacacctt tcaacgggat aacgccaccg cgactgtcag
     8941 gtctgagacg tggagtggca tggctcaggg cggacgcccg gcctgaggcg tgaaaccgtc
     9001 gaagaggtac gttcctgcca tgcacaccgt cgccgtcatc gcgctggatc aggtgatccc
     9061 gttcgacctg tcaaccccgg tcgaggtctt cacgcggacc cgtctgcccg atgggcgacc
     9121 tggctaccag atccgtgtgt gcgccgagga cgaggagatc gacgccggca ccttcactct
     9181 tcgtgccccc tggggtctgg agggcctcca ggacgcggac acgatcatcg tgccgggtgt
     9241 cgctgatccc gcggccccgc tctctccccc ggtacgcgac gcgttgcggt cggccgccgc
     9301 cgacggcacg cggatcgcgt cgatctgcac gggcaccttt ccgcttgccg ccaccggcct
     9361 cctcgacggg ctgcgggcaa ccactcactg gcttgcggcc ggactgctgg ccgcaagcca
     9421 tccggacatc gacgtcgacc cggacgtcct ctacgtcgac agcggtcagt tcctcacctc
     9481 agccggcgca gccgcaggca tggacttgtg cctgcacatg atccgccggg actacggctc
     9541 ggccgtcgcc gccgatgccg cccggctgtc ggtgatgcct ctcgaacgag agggcggaca
     9601 ggcgcagttc atcgtccacg accacgctcc gaccccgcag gggtccgacc tcgaaactct
     9661 gctcacatgg ctgcgcagca acctgggccg cgacctcacc ctcgctgaca tcgcggcgca
     9721 gtccgggaca agtacccgga ccctgatccg ccgcttccgc gaacagacgg gcaccacccc
     9781 gctccagtgg ctccatcgag cccgcatccg ccaggcccag cacctgctcg agaccacgga
     9841 gcattccgtc gaacgcatcg gcacccaggt cggattcggc tcggcgacca ctttccgtga
     9901 ccgcttcaaa cgcacgaccg gcgtcagccc gaagacctac cggcgcacgt tcagctgaga
     9961 cgtggtcacc gaaccggatg cagcttcgag gccggaaaaa cggcaacggg cggctgcctt
    10021 gtgacacccg accggagcca cgcgcgcctg cacgacctgc cggaccgacc agccctggac
    10081 cggccctaga ccagcccggc caccagctcc gccacgggct tgcgccgccc cgtgtagaac
    10141 ggcacctctt cccgtacgtg catccgcgcc tccgagcccc gcaggtggcg catcagatcg
    10201 acgatgcggt gcagctcgtc ggcctcgaag gcgaggatcc actcgtagtc gccgagggag
    10261 aaggcgggca ccgtgttggc ccgtacgtcc gggaacccgc gggccatctt cccgtggtcg
    10321 gcgagcatgc ggcggcggtc ctcgtccgga agcagatacc agtcgtagga acgcacgaag
    10381 gggtacacgc tgacgtaggc ccgcggttcc tcaccggcga cgaaggcggg caggtgcgac
    10441 ttgttgaact cggcggggcg gtgcagcgcc atgttcgacc agaccggttc cagggcccgc
    10501 cccaagcggg tgcggcggaa gcggttgtag gcgtcctgca gggcgtcgga ggattccgcg
    10561 tgccaccaga tcatcaggtc ggcgtcggcg cgcagccccg agacgtcgta ggtgccgcgc
    10621 accaccacgt ccttcgcggc gagctgcccg aacagctcct ccacctcgtc cgcgtatccc
    10681 gcgcggtcct cgggcaggac gtcgcgcagc cggaagaccg accacagggc gtatcggacg
    10741 acctcgttga ggtccttggc cttcttgccc gcgttgggcg tcttctcggg gctcccaggc
    10801 tccggtgcag ctgtcatgcg cctattctcg cgcgctgccg gccgtcgccg gcgcctgggt
    10861 ctccctggcc gcacgctcac cgctcgcaat gcacgcgggg atgcccaccc cgtcgtacgc
    10921 ggccccgcac agcgccaggc ccggcacctt ctccacggcc tgacggatgc gggcgacgcg
    10981 cgccctgtgg ccgaccgggt actgcggcag gccgccgctc cagcgggtta ccacgcggtc
    11041 caccggggag gcggacagac ccgtcgcggc agccagatcg cgcagggaca gctcaacgag
    11101 ctcggagtcc tcgcgctcga ggaccgcctc ctcgccgtac cgcccgacgg aggtccgcag
    11161 cacgaacagt tccgggtcct gtgcggcgac ccacccccac ttgttgctgg agaaggtgga
    11221 cgccttgatg gtgcggccgt cgaccggggg cacgaggaag ccgctgccgc ccggcacgtg
    11281 atcgaggtcc gcgcgccgga aggccatggt gacca
//
