LOCUS       NZ_CP048882            10999 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Streptomyces bathyalis strain ASO4wet chromosome, complete genome.
ACCESSION   NZ_CP048882
VERSION     NZ_CP048882.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces bathyalis
  ORGANISM  Streptomyces bathyalis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7377472)
  AUTHORS   Risdian,C., Landwehr,W., Rohde,M., Schumann,P., Hahnke,R.L.,
            Sproer,C., Bunk,B., Kampfer,P., Schupp,P.J. and Wink,J.
  TITLE     Streptomyces bathyalis sp. nov., an actinobacterium isolated from
            the sponge in a deep sea
  JOURNAL   Antonie Van Leeuwenhoek 114 (4), 425-435 (2021)
   PUBMED   33595745
REFERENCE   2  (bases 1 to 7377472)
  AUTHORS   Risdian,C., Landwehr,W., Schupp,P. and Wink,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2020) Microbial Strain Collection, Helmholtz
            Centre for Infection Research, Inhoffenstrasse 7, Braunschweig,
            Niedersachsen 38124, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-FEB-2019
            Assembly Method                   :: HGAP v. v3
            Assembly Name :: Streptomyces_DSM106605_HGAP3_1SC_10M
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 117.0x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910445.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 04:35:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,467
            CDSs (total)                      :: 6,387
            Genes (coding)                    :: 6,276
            CDSs (with protein)               :: 6,276
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 37 of 111
            Pseudo Genes (incomplete)         :: 90 of 111
            Pseudo Genes (internal stop)      :: 12 of 111
            Pseudo Genes (multiple problems)  :: 25 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:44:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6971
            Orig. end                         :: 17970
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from MISG, Helmholtz Centre for
            Infection Research, Germany.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10999
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10999
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10999
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5999](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5999
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(592..605)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.41"
                     /tool="antismash"
     gene            complement(955..1440)
                     /locus_tag="G4Z16_RS00045"
                     /old_locus_tag="G4Z16_00045"
     CDS             complement(955..1440)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989523.1"
                     /locus_tag="G4Z16_RS00045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_00045"
                     /product="DoxX family protein"
                     /protein_id="WP_197348543.1"
                     /transl_table=11
                     /translation="MNQSPGAPSGSPASASGPAPVPARAARHDRVKHISLLALQVILGL
                     FFVIASAVPKLIAHSSAVEGFEEIGFGMWFMYLIGVLELAGGIALATPWLSGLSALSLI
                     GLMIGAFVMQLTVFDGEYAATPLLLIVPLALIAWGRRDTLTALPGLLRGNGRGATAS"
     gene            complement(1635..2108)
                     /locus_tag="G4Z16_RS00050"
                     /old_locus_tag="G4Z16_00050"
     CDS             complement(1635..2108)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /locus_tag="G4Z16_RS00050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_00050"
                     /product="DoxX family protein"
                     /protein_id="WP_197348544.1"
                     /transl_table=11
                     /translation="MANASGIPPTSHAGSPAARSSRPKGPARIALEVALWAIQLYLAYF
                     MITVGAIPALTADSGAKGTFEDIGFGLWFMYLTGALELLGAIGLITPWFSGLAALGLMF
                     VMSGACLTHLTLYDGKGVETPAMILIPLALVVVGRWSTVKKLGTWLTGGVDRR"
     gene            complement(2447..3121)
                     /locus_tag="G4Z16_RS00055"
                     /old_locus_tag="G4Z16_00055"
     CDS             complement(2447..3121)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF041196.1"
                     /locus_tag="G4Z16_RS00055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_00055"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_197348545.1"
                     /transl_table=11
                     /translation="MRQERAELTRQALTMAAAKSFDRLGYQRTTLFEVSRGAAMSKGAL
                     SFHFGSKEELADTVRREACRRSNQAAELLRAKEQPALQTLIDLTHLVAHQLEHDHLARA
                     AVKLARELDTPPDPEINPLACWQQVFTDVAREAGKDGTLRCEATTEAVAELAFCLASGA
                     HSVQTNGTDDSAVDVHQRLAAHWELLLHGLVTPPGPGELWSAGTEADWALATQGGQPSP
                     AH"
     misc_feature    complement(3213..3233)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.62"
                     /tool="antismash"
     misc_feature    3229..3238
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.0"
                     /tool="antismash"
     gene            complement(3331..4272)
                     /locus_tag="G4Z16_RS00060"
                     /old_locus_tag="G4Z16_00060"
     CDS             complement(3331..4272)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742094.1"
                     /locus_tag="G4Z16_RS00060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_00060"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_246530576.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.6e-14, bitscore:
                     52.2, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALTGASGFIGSAVLRRLARHDVDVRALTRQEHPLPAEVEQVHAD
                     LARPESLDGSCSGARVLIHAASYVGLDEQLATEVNDLGTEALMKEARRAGVERIVQLST
                     TAVYGRGPHRGAAVGELEPDPGSAASRSRLAGEEHALRQGAAVLRAGLITGRGDRWVVP
                     ALAELVRRVPGFWRAGEAQLSLIDVDDLARLVTLTALQLAGHRGSSGPVRGVHHAVHPV
                     PVRNRDLLETLAGLDVLPPVTGELAWDECLALMDENPGRISTSQLTLLAFDHHYATDVW
                     QACDAPPGPGPLAALTEAAPWYRTHLAGTARP"
     gene            complement(4281..4898)
                     /locus_tag="G4Z16_RS00065"
                     /old_locus_tag="G4Z16_00065"
     CDS             complement(4281..4898)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986960.1"
                     /locus_tag="G4Z16_RS00065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_00065"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_197348547.1"
                     /transl_table=11
                     /translation="MQQRAVETRQLILEAAAEVFDEGGYKAARISEIMHRAGITQGALY
                     FHFSSKLDLARTVMLAQRDSVVLPPGPDGLQRLIDTTLLLAHELQTNKLLRAGVRLAVE
                     QEEVGFHDDSPYREWAEVFRGQLAAAAERGQLRSGVDVERLAMLLVGCYTGVQHFSKLS
                     CKRADLPDRIADLWQYMLLGIAPPQVAADMIVSTEAPLLAAG"
     gene            5001..5999
                     /locus_tag="G4Z16_RS00070"
                     /old_locus_tag="G4Z16_00070"
     CDS             5001..5999
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="G4Z16_RS00070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_00070"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_197348548.1"
                     /sec_met_domain="AfsA (E-value: 5.1e-21, bitscore: 73.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAHTPAVPDLPESTAYSWPVPREFVHKTAHAEVLLTGWRRCGERE
                     FEVGAQWPRDHGFWRTDEYGVQDPMLLVETTRQVLPLIAHAAYGVPSGHQLIWDHHHCA
                     FSTPFLPVTGSPAEVVLHLSASEAPDRGMRPRRIGLSMTVRSGDRTLGSASTVYTAHSP
                     QVYGRLRGPKTPAPPDAMAAALTPPPALTPSLVGRESEREVVLAPSADRSMGRRQLRVL
                     TTSPWLFDHPVDHAPGMLLMEAARQSVHLATSPRRPSLTRMEAEFHRYVDLDAASWVEV
                     SSGPDGSGRGDRVGGERTLTVSITQHGEKAFTALVGFTTQAGATATAASAR"
     misc_feature    5328..5330
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6161..7015
                     /locus_tag="G4Z16_RS00075"
                     /old_locus_tag="G4Z16_00075"
     CDS             6161..7015
                     /GO_function="GO:0008276 - protein methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718074.1"
                     /locus_tag="G4Z16_RS00075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_00075"
                     /product="putative protein N(5)-glutamine
                     methyltransferase"
                     /protein_id="WP_197348549.1"
                     /transl_table=11
                     /translation="MTATPPSFPSLPSRSALAARLRDAGCVFAEDEADLMLSAARTAEE
                     LAGMAERRAEGFPLEHVLGHAEFGGLRIVVEPGVFVPRRRTEFLVRRAAALTRPGALVL
                     DLCCGSGALGVALAAAVARSGQEPCGGVELHAADIDPAAVRCARHNVAAVGGSVHEGDL
                     YEGLPPELRGRIEVLIANVPYVPTDSLSLLPSEARVHELRAALDGGRDGLDMLRRVAAS
                     APEWLAPGGHLLMETTEAQVEAATEAVEGSGLTVRVDRCEELGATVLTGTKQRGAQAST
                     CPR"
     gene            complement(7012..8511)
                     /locus_tag="G4Z16_RS00080"
                     /old_locus_tag="G4Z16_00080"
     CDS             complement(7012..8511)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003766.1"
                     /locus_tag="G4Z16_RS00080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_00080"
                     /product="cytosine permease"
                     /protein_id="WP_281393767.1"
                     /transl_table=11
                     /translation="MEANGLDVIADAERKGRPAQLFWPWFGSNVSVLGLSYGSFALGFG
                     IGFWQALAAGIVGIVFSFLLCGFIAVAGKRGSAPAMVLSRAAFGVRGNRLPSAVSWVLT
                     VGWETVLVILATLATATVFDRLGWGGGTGTKIAALVVVSVLTVLAGVIGFDLIMRMQTA
                     ITVVTAALTVVYVVLAADEIHWESVRALPSGSAQEVIGALVFLMTGFGLGWVNAAADYS
                     RYLPRRTRGAAVAGWTAFGASAAPVVLLVFGLLLAGSSKDLSEAIAADPVGALTGALPG
                     WFLVPFALVAVLGLVGGAVLDIYSSGLALLALGVRMPRWAAAAVDGVLMVAGTVYIVFF
                     SEGFLGSFTGFLTTLGVPVAAWCGIMLADIALRSRPYDDGDLFSRNGRYGDVRWLPVAV
                     LAVTTAAGWGLVTNAAAGWLSWQGYLLGPLGLGGTAGGWASANLGVLAALVLAFAVTLL
                     TQRGRIRAQESAPPSLPPGPVREPGEAVDGTGTSASAATGA"
     gene            9132..9416
                     /locus_tag="G4Z16_RS00085"
                     /old_locus_tag="G4Z16_00085"
     CDS             9132..9416
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="G4Z16_RS00085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="G4Z16_00085"
                     /product="hypothetical protein"
                     /protein_id="WP_197348551.1"
                     /transl_table=11
                     /translation="MSGTLCTATLRVLHYSLCARVTDERTQFYLDLNAVQRGDALPTAE
                     LPGLLPPGSRLRFHIVGAHESFRVPLGADARCRFHSDVASAWAEWSRQP"
     gene            complement(9498..9908)
                     /locus_tag="G4Z16_RS00090"
                     /old_locus_tag="G4Z16_00090"
     CDS             complement(9498..9908)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430555.1"
                     /locus_tag="G4Z16_RS00090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_00090"
                     /product="ATP-binding protein"
                     /protein_id="WP_246530577.1"
                     /transl_table=11
                     /translation="MSPRPSRRTARVEYTLPRNAASARTARQLTTGFLTGSRTPPEAAG
                     QVEEATLVVSELVTNAALHGRGRCRLRLSSGEGTVTVEVRDDGGLLPRVRRAGSDSESG
                     RGIAMISRLARRLDVTTAPGGGKTVSAVLDAA"
     gene            complement(10149..10529)
                     /gene="mnhG"
                     /locus_tag="G4Z16_RS00095"
                     /old_locus_tag="G4Z16_00095"
     CDS             complement(10149..10529)
                     /GO_function="GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mnhG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358520.1"
                     /locus_tag="G4Z16_RS00095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_00095"
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="WP_197348553.1"
                     /transl_table=11
                     /translation="MNWNQHNWSEAADVASLVCMLAGAVLCLLAGIGLVRLPDVLTRIH
                     AATKPQVAGLLLVLLGAGLRLRDVADLGTLVLVGLFQLLTVPVAAHMVGRAAYRTGRTD
                     REHLVVDELGTALDKSRGDQGE"
     gene            complement(10526..10921)
                     /locus_tag="G4Z16_RS00100"
                     /old_locus_tag="G4Z16_00100"
     CDS             complement(10526..10921)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358521.1"
                     /locus_tag="G4Z16_RS00100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="G4Z16_00100"
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="WP_197348554.1"
                     /transl_table=11
                     /translation="MEAVLSIVIALLLASGVLVTVRLVRGPSTLDRAVALDALAAVVMA
                     GIGVLTAARQIPYYLPALLVLSFLGFTGSVSIARFLALRDEAGSEDPDLGDGRGGENDG
                     PESAAPDRGRGGGPEQNGGAAEQGSGG"
     misc_feature    10940..10952
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 18.92"
                     /tool="antismash"
ORIGIN
        1 cgcgtgcagg tggacagcgc cgtgctgtac gtggaggacg ggcccgtgct caccagcgcg
       61 ggagcggcgg cggggataga cgcctgcctg cacctggtgc gccgtgagca cggtgcggcc
      121 accgccaacg cactggcgcg gcgcatggtc gtgccggccc atcgcgcggg cgggcaggcg
      181 cagttcgtcg agatgcccct gcccgaggcc cgcgccgggt acggcatgac ggagctggtc
      241 gactggatcc aggcgcatct ggaccggccg ctgaccgtgg aggatctggc cgcgcacgtc
      301 gccatgtcgc cgcgcacgct ggcccggagg ttcaaggagg cgaccggcac gaccccgcac
      361 cggtggatcc tcgaccagcg tctccagctc gccgaggagt tgctggagaa cacgtcgctg
      421 ccggtggagg ccgtggcggc ccgttccggt ttcggcagcg ccgacacctt gcgtcatcat
      481 ttcgccgccc gccgcggcgt cgcccccgct gcgcaccgcc ggacgttcca cgccggcact
      541 ccgccggtga gcggcgagca caaccaggcc cgacgaaagg accggagtat ggagatctgg
      601 atcaaccccg cctgttccaa gtgccgttcg gcgctgtcac tgctggacgc ggagggcgcc
      661 gagtacacgg tccgccgcta cctggaggag ccgccgagcg tgcgggagct ggaagacgta
      721 ctgctgcggc tgggcctgga gccctgggac atcacccgca ccggcgaacc cgccgcgggc
      781 gagctgggca tggagtcctg gcctcgcgat gcggacaacc gcacccggtg gatcgacgcg
      841 ctggcggccc acccctccct gatccagcgg ccgatcatca ccgctgacga cgggagtgcg
      901 gtggtgggcc gctcaccgga gtcggtcaag tccgtactgg gatgaggtct gcgctcaact
      961 ggccgttgcg ccacgtccgt tgccccgcag cagccccggc agtgccgtga gggtgtccct
     1021 gcggccccat gcgatgaggg cgagcggcac gatcagcagc agcggcgtgg cggcgtactc
     1081 gccgtcgaag acggtcagtt gcatgacgaa cgcaccgatc atcaggccga tgagtgagag
     1141 cgcggagaga cctgagagcc acggcgtggc cagggcgatc ccaccggcca gttccagtac
     1201 cccgatcaag tacatgaacc acatgccgaa gccgatctct tcgaagccct cgaccgcgga
     1261 ggaatgggcg atcagcttgg gaacggcact ggcgatgacg aagaacaggc cgagtatcac
     1321 ctgcagcgcg agcagggaga tgtgcttgac gcggtcgtga cgggccgctc gggccgggac
     1381 gggcgccggg cccgaggcgg atgccgggga tccggagggt gctccgggtg actggttcat
     1441 gacggtctcc tctcaggggc gcgtacggga cgtacacacc cggagcagac ccggctgccg
     1501 cacgtttctc atcgcggcga cggcagcctg ttcacacgcg ctcgcgtgcg gcgcggagcg
     1561 tgcccgaaag cctgggaggc gcagctcggc cggtgtccgt tctcgccgga tcccggtcgt
     1621 gctgcgccgt cggctcagcg ccggtccacg cctcccgtca gccacgtgcc cagcttcttc
     1681 accgtcgacc accggccgac gacgacgagg gcgagcggga tgaggatcat ggccggggtc
     1741 tcgacgccct tgccgtcgta gagcgtcagg tgcgtgaggc acgcgcccga catcacgaac
     1801 atcaggccga gcgcggcgag gccagagaac cacggggtga tgagaccgat ggcaccgagc
     1861 agttccagtg ccccggtcag atacatgaac cagaggccga agccgatgtc ctcgaacgtg
     1921 cccttggcgc cggagtcggc ggtcagcgcc ggaatcgccc cgacggtgat catgaagtag
     1981 gcgaggtaga gctggatggc ccacagggcg acttcgaggg cgatgcgggc cggacccttg
     2041 ggacgggagg aacgggcggc cggtgaacct gcgtgggagg tcggcggaat gccgctcgcg
     2101 ttcgccatga tggactcctt ctgcggacgg tgcacaagcg gttgaccggg gcagcccggc
     2161 agctgacctt ctaccaccgg cacacccgcg gcggaactcc ctcgccacag cggaagcgag
     2221 ggctgcaccc gtgcgcggag cgccggggct tgtcatttgc gtatcacgtg cccggtaagc
     2281 agcgcagaca gttgtccatc atggtcaaca tcccggcctg cgggggcgtc cctcgcggcc
     2341 gggccatcgg atcctgcgta ctctccgtga ccgttgaggg cggtcaccgt gactcttcac
     2401 cacgggctcc ccgccgtgtc tcacaggcgg ggcgcgaccg gtcggctcag tgtgcggggc
     2461 tcggctgccc gccctgcgtg gccagcgccc agtccgcctc ggtgcccgca ctccacaact
     2521 cgcccgggcc cggcggcgtc acgagtccat gcagcagcaa ctcccagtgg gcggcgaggc
     2581 gttggtggac atcgacggcc gagtcgtccg tcccgttcgt ctgcaccgag tgcgcacccg
     2641 aggcgaggca gaaggcgagt tcggcgacgg cctccgtggt ggcctcgcag cgcagcgtgc
     2701 cgtccttccc cgcctcacgt gcgacgtcgg tgaagacctg ctgccagcag gccagcgggt
     2761 tgatctcagg gtcgggaggg gtgtcgagtt cgcgcgcgag ctttaccgcg gcccgcgcca
     2821 ggtggtcgtg ctcgagctga tgcgcaacga ggtgcgtcag atcgatgagg gtctgcaggg
     2881 ccggctgttc cttcgcccgc agcagctcgg cggcctggtt cgaccgcctg cacgcctcac
     2941 gccgcacggt gtctgccagt tcctccttgc tcccgaagtg gaaggagaga gcgcccttgc
     3001 tcatcgctgc gccgcggctg acctcgaaca gtgtcgtacg ctgatagccg agccggtcga
     3061 acgacttcgc ggccgccatc gtcagtgcct gacgggtgag ttctgctcgc tcttgcctca
     3121 tcggctgtgt cccttcatgc gttccggtcc gcggggagac gggccgtcag gagcggcggc
     3181 tgtgggggga gatgagagct gccgtcccca caaacataca aagtgttctg tttttttgcg
     3241 ccagaagggc tgactccgtt cgttgacgga gagcgtcaag gggtgcgcta tgtggccagt
     3301 gggacgggtg gtaccggagg ggccgcgccg tcaggggcgg gcagtaccgg cgagatgggt
     3361 gcggtaccag ggggccgctt cggtgagagc tgccaacggc cccggccctg ggggcgcatc
     3421 gcacgcctgc cacacatcag tggcgtagtg gtggtcgaag gcgagcagtg tgagctggct
     3481 cgtgctgatg cgtcccgggt tctcgtccat gagggcgagg cattcgtccc aggccaactc
     3541 ccctgtgacg gggggcagta cgtccaggcc agcgagcgtc tccagcaggt cccggttgcg
     3601 cacggggacg gggtgcacgg cgtggtggac gccccggacc gggccggagg agccgcggtg
     3661 cccggcgagt tggagcgcgg tcagcgtcac gagccgcgcc agatcatcca cgtcgatgag
     3721 cgacagctgt gcctcccctg cccgccagaa ccccggtacg cggcggacga gttcggccag
     3781 cgcggggacg acccagcggt ctccccgacc ggtgatcagg ccggcccgca acaccgcggc
     3841 accctggcgc agggcgtgct cctcgcccgc cagacggctg cggctcgccg cggatccggg
     3901 atcgggttcc agctctccga cggccgcgcc gcggtggggc ccacgcccgt acacggcagt
     3961 ggtggacagc tgcacgatgc gctccacgcc ggcgcgccgc gcctccttca tcagtgcttc
     4021 cgtgcccaag tcgttgacct cggtggccag ttgctcgtcg aggcccacgt aggaagcggc
     4081 gtggatcagg acgcgcgcac ctgaacaact gccatccagg gactcgggac gggccagatc
     4141 ggcgtggacc tgttccacct cggcgggcag cggatgctcc tggcgcgtga gggcccgtac
     4201 gtccacatcg tgccgtgcca gacgccgcag gacggccgaa ccgatgaagc cgctcgcccc
     4261 cgtcagtgcc acacgcaccg tcatccggcg gccagcagcg gggcttcggt gctgacgatc
     4321 atgtccgccg cgacctgcgg tggagcgatg cccaggagca tgtactgcca caggtccgcg
     4381 atgcggtcgg gcaggtctgc ccgcttgcac gagagcttgg agaaatgctg tacgccggtg
     4441 tagcagccga ccaggagcat cgcgaggcgt tccacgtcga ctccgctgcg cagctgccct
     4501 cgttctgcgg cggcggcgag ctggcctcgg aagacctccg cccactcacg gtacggggag
     4561 tcgtcgtgga agccgacttc ctcctgctcc accgccagcc ggacccccgc acgcaggagc
     4621 ttgttggtct gaagttcgtg cgccagaagg agggtcgtgt cgatcaggcg ctggagcccg
     4681 tcgggtccgg gaggcaggac gacggagtcg cgctgcgcga gcatgacggt acgggcaagg
     4741 tccagcttcg aggagaagtg gaagtagagg gcaccctgag tgatgccggc ccggtgcatg
     4801 atctccgaga ttcttgccgc cttgtagcct ccctcgtcga aaacttctgc cgctgcttca
     4861 aggatcaact gccttgtctc gacagctcgt tgctgcatcg gcttggatct tgcttgcacc
     4921 atctgacctc gcccccagaa ggttctatcg ttttcaggca caggtcaccg tcatccttgc
     4981 accgtccccc ggaggaccgc atggctcaca cccccgctgt gcccgatctc ccggagagca
     5041 cagcgtactc gtggccggta ccgcgggagt tcgtacacaa gacggctcac gcggaagtgc
     5101 tcctgacggg ctggcgtcgg tgcggggagc gcgagttcga ggtgggtgcc cagtggccgc
     5161 gggaccacgg cttctggcgg acggacgagt acggcgtaca ggatccgatg ctgctggtcg
     5221 agacgacccg gcaggttctg ccgctgatcg cgcatgccgc ctacggggtg ccgtcgggac
     5281 accagctgat atgggatcac caccactgcg ccttctccac gccgttctta cccgtgaccg
     5341 gctcccctgc cgaggtcgtg ctgcacctgt cggcctcgga ggcccctgac cgcggcatgc
     5401 ggccgcggcg gatagggctg agcatgaccg tccggagtgg cgaccggacc ctgggcagcg
     5461 cctccaccgt gtacaccgcg cacagcccgc aggtctacgg cagactccgc gggcccaaaa
     5521 cacccgcacc cccggacgcc atggccgccg ctctcacacc gccgccggct ctgactccct
     5581 cgctggtggg ccgggagagc gagcgcgagg tggtgctcgc tccgtccgcc gacaggagca
     5641 tggggcgtcg gcagctgcgg gtcctcacaa caagcccctg gctcttcgac caccccgtcg
     5701 accacgctcc cggcatgctg ctgatggagg cagcacggca gtcggtgcac ctggccacat
     5761 caccgcgccg gccgagcctg acccgtatgg aggcggagtt ccaccgctac gtcgatctcg
     5821 acgccgccag ctgggtcgag gtctcatcgg gcccggacgg ctccgggcgc ggcgaccggg
     5881 tcggtgggga gcgcacgctg acggtgagca tcacgcagca cggcgagaag gcattcaccg
     5941 cgctcgtcgg cttcaccacc caggccggcg ccaccgcgac cgccgcgtcg gcccggtagg
     6001 gttgccgacc cggccgcaca catctcccgg ggaggtggga ccgatcaccg ctgtggaagg
     6061 cggtgctcct ctcctctccc cagggcctgc cttcccgctg gtccaggccc tgcggagccg
     6121 gagccggaag cacctccgga atcccgagag gcccgcccgt atgactgcga ctcccccgtc
     6181 cttcccctca ttgccctccc gctccgccct cgccgccagg cttcgcgacg ccggctgtgt
     6241 cttcgccgag gacgaggccg acttgatgct ttccgcggcc cgcacggcgg aggaactcgc
     6301 ggggatggcg gagcgcagag ccgaaggatt cccgctcgaa cacgtcctgg gccacgcgga
     6361 gttcggcggt ctgcggatcg tggtggagcc gggggtcttc gtccctcgcc ggcgcacgga
     6421 gttcctcgtg cggcgcgccg cggcgctcac acgtccaggg gcccttgtcc tcgatctgtg
     6481 ctgcggctcc ggcgcgttgg gtgtggcgct ggcggcggcg gtcgcacggt ccggtcagga
     6541 gccttgcggc ggggtcgagt tgcacgccgc cgacatcgac ccggcggcgg tgcgctgcgc
     6601 gcggcacaac gtcgcggccg tcggcggcag tgtccatgag ggggatctct acgaaggact
     6661 gccgcccgag ctgaggggcc gcatcgaggt gctgatcgcc aacgtcccct acgtaccgac
     6721 ggattcgctg agtctgcttc cgtccgaggc acgcgtccac gagcttcggg cggcgctgga
     6781 cggcggcagg gacggactgg acatgctgcg tcgggtcgcg gcgtcggcgc cggagtggct
     6841 cgccccgggc ggacatctgc tgatggagac gaccgaggcc caggtcgagg cggcgaccga
     6901 agctgtggag ggcagcgggc tgacggtgcg cgtggaccgt tgcgaggagc tgggcgcaac
     6961 ggttctcacc gggacgaagc agcgtggggc gcaggcgtca acctgccctc gctaggcgcc
     7021 tgttgcggcg gaggcggaag taccggtgcc gtccacggct tccccgggct cccttaccgg
     7081 tcccggcggc agggagggtg gtgcgctctc ctgcgcccgg atcctgccgc gttgagtcag
     7141 cagcgtcacc gcgaaggcaa ggacaagggc ggcgagcaca ccgaggttcg cggacgccca
     7201 cccgcccgcc gtgccgccca gccccaaggg ccccagcaga tagccctgcc aggagagcca
     7261 gccggcggcg gcgttggtca ccaggcccca gcccgcggcc gtcgtcacgg ccaggaccgc
     7321 gacgggcagc caacgcacgt cgccataacg cccgttgcgc gagaagaggt cgccgtcgtc
     7381 gtacgggcgg gagcgcaggg cgatgtcggc gagcatgatg ccgcaccatg ccgccaccgg
     7441 cacgccgaga gtggtgagga atccggtgaa ggagccgagg aacccttcgg agaagaacac
     7501 gatgtagacg gtcccggcca ccatcagtac accgtcgaca gcggcggcgg cccagcgcgg
     7561 catccgcact ccgagtgcca gcagcgccag gccggacgag tagatgtcga gtacggcgcc
     7621 gccgacgagt cccagcacgg cgacgagcgc gaacggtacg aggaaccacc ccggaagggc
     7681 gcccgtgagg gcaccgacgg gatctgccgc gatcgcctcg ctgaggtcct tcgaggagcc
     7741 cgcaagcagg agaccgaaga cgagcagaac caccggggcc gcggacgcgc cgaacgcggt
     7801 ccagcccgcg acggccgcgc cccgggtgcg acgcggcagg taacgggagt aatccgccgc
     7861 cgcgttgacc cagccgaggc cgaagcccgt catcaggaac acgagcgcgc cgatgacctc
     7921 ctgcgccgat ccggagggca gcgctcgcac ggactcccag tggatctcgt cggcggccag
     7981 gacgacgtag acgacggtca gcgcggccgt caccacggtg atggccgtct gcatccgcat
     8041 gatcaggtcg aacccgatca ccccggcgag cacggtgagc accgagacga cgaccagggc
     8101 ggcgatcttc gtgcccgtgc ctccgcccca gcccagcctg tcgaagacgg tcgccgtggc
     8161 gagcgtcgcg aggatcacca gcacggtctc ccagccgacg gtcaggaccc aggagaccgc
     8221 ggagggaagc ctgttgcccc gtactccgaa cgcggcgcgg ctgagaacca tcgccggtgc
     8281 ggatccgcgt ttccctgcca ccgcgatgaa tccgcagagc aggaaggaga agacgatgcc
     8341 cacgatgccg gcggccagtg cctgccagaa cccgatgccg aaaccgagcg cgaacgagcc
     8401 gtaactcagg cccagcaccg agacgttgga tccgaaccac ggccagaaca gctgggcggg
     8461 ccggcccttg cgctcggcgt cggcgatgac gtcgaggccg ttggcctcca cggcgatcct
     8521 ccccgaggag ttgtcccgta cctcgccatg ctgtgaatcc gccgcctcag tgccaggcac
     8581 agcgcccccg ttccgccggt tccgtcgcat ggagcctgct ggcgccttcg tgcggcgtca
     8641 agtacgcgcc gaggccgtgc cgttgggcgt ctgcgtcaag ggaagccggg gaagcctgcg
     8701 gctgccgcgt tcactccgga ggcaaccacc aggtgccggt gcctccgcgg cgagttgggc
     8761 attcaccgca accgcgttcc gccatgacca gcggtcctgc ccaaccgcgc cgctgcggca
     8821 gagcggctcg tggccggccc ctgtgcgacc ttcgccggcg gaccagccgg cgggaaccgg
     8881 ggtgttcccg tgaggaaatg cggggagaac gtgccggtcg gtgacccacc tgcccgattg
     8941 ctgtaccgcc cgtgatagtt gtgtgcgcca acgagaacgg cagctacacc gaatgatcag
     9001 tacggtccgg agcgggtacg ggtgttgctc gtcgcgcgcc ggaacgtacc gtctggacaa
     9061 gggctttcat ggggacgccg acgatacagg tccgagtcct tgccgtcgcg ggccgacgtg
     9121 tgtggatggc ggtgagcggc acactgtgca ccgcgacgct cagggtcctg cactacagtc
     9181 tgtgcgcccg cgtcacggat gagcggacgc agttctatct cgacctcaac gcggtgcagc
     9241 gcggcgacgc gctccccacc gccgaacttc ccgggctgct gccgcccggc agccgtctgc
     9301 gcttccacat cgtcggagcg cacgagagtt tccgggtgcc gctcggagcg gacgcgcgat
     9361 gccgcttcca ctcggacgtc gcctccgcgt gggcggagtg gtcgcggcag ccctgagagg
     9421 cgagcaaccg ggcctgaaca ggcccgagtc ggcgggaatc ggccggaggt gcttgaggtc
     9481 cggcagccgg aggagcgcta cgcggcgtcc agtacggcgc tcactgtttt gcctccacca
     9541 ggtgcggtgg tgacgtccag acgcctggcg agccggctga tcatcgcgat gccccgcccg
     9601 ctctcggagt cactccccgc gcggcgaacg cggggcaaca ggccgccgtc gtcgcggact
     9661 tcgacggtga cggttccctc gccactgctc agccgcagcc ggcaccgccc cctgccgtgc
     9721 agcgccgcgt tggtgacgag ttcggagacg accagtgtgg cctcttccac ctgcccggcc
     9781 gcctcgggcg gcgtgcgaga gccggtgagg aatccggtcg tcaactggcg ggcggtgcgg
     9841 gccgacgccg cgttccgcgg cagtgtgtac tcgacgcgtg ccgtgcgccg cgagggacgc
     9901 gggctcacgc gcatgacgct cacttcccct cggttccggc tcccaccccg tgcgagcggg
     9961 gcggccggat gcgcgaatcc agaccttcgg gtgttcttcc cttggggcgc gggagcattc
    10021 tcagggaaac cggctcggac gcatccggtc acggaaaatc caccaatccg tcgcgttccg
    10081 ctcgaaagca ggccgtaccc tccgcactgc cgggaagtgt ccgtccgccg accacccgtt
    10141 cccgtcggct attcgccctg gtctccgcgg gacttgtcca gcgccgtgcc gagttcgtcg
    10201 acgacgagat gctcacggtc cgtcctgccg gtgcggtagg cggcccgtcc gaccatgtgc
    10261 gccgccaccg ggacggtcag caactggaac aggccgacga ggaccagcgt tcccaggtcc
    10321 gccacgtcgc gcagccgcag cccggcaccc aggagcacaa gcagcagacc cgccacctgc
    10381 ggcttggtcg ccgcatggat gcgtgtgagg acgtccggca gccgcaccaa tccgatgccg
    10441 gcgagcaggc agagcacggc gcccgccagc atgcagacca gggacgccac gtccgcggcc
    10501 tccgaccagt tgtgctggtt ccagttcatc cgccgctccc ctgctccgcc gcgccgccgt
    10561 tctgttcggg gccgccgccc cgtccgcggt ccggggcggc actctccggg ccgtcgttct
    10621 ctccgcccct tccgtcgccc aggtcgggat cctcgctccc ggcctcgtcc cgcagcgcga
    10681 ggaagcgggc gatgctcacc gatccggtga agcccaggaa ggagaggacg agcaacgccg
    10741 ggaggtagta cgggatctgc cgcgccgccg tgagcacgcc gatgccggcc atcaccaccg
    10801 cggccagcgc gtcgagggcg acggcgcggt ccagcgtcga gggtccgcga accagccgca
    10861 cggtgacgag caccccggag gcgagcagca gggcgatgac gatgctcagg accgcttcca
    10921 cggaccgctc cccccatcgg tgtcactgtc gccgcctgtg ccgcctccgg ggccaccgcc
    10981 tccggcgttg ccgcctcct
//
