LOCUS       NZ_LDHL01000015        10312 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Bacillus mycoides strain G25-104 G25-104_contig000015, whole genome
            shotgun sequence.
ACCESSION   NZ_LDHL01000015
VERSION     NZ_LDHL01000015.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 1940029)
  AUTHORS   Zheng,Z., Zheng,J., Zhang,Z., Peng,D. and Sun,M.
  TITLE     Nematicidal spore-forming Bacilli share similar virulence factors
            and mechanisms
  JOURNAL   Sci Rep 6, 31341 (2016)
   PUBMED   27539267
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940029)
  AUTHORS   Zheng,Z. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2015) Microbiology, Institute of Life Sciences and
            Technology, Shizishan str., Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.3.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 170.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015845385.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 01:09:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,771
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,404
            CDSs (with protein)               :: 5,404
            Genes (RNA)                       :: 167
            rRNAs                             :: 14, 16, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 16, 15 (5S, 16S, 23S)
            tRNAs                             :: 117
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 85 of 200
            Pseudo Genes (incomplete)         :: 137 of 200
            Pseudo Genes (internal stop)      :: 43 of 200
            Pseudo Genes (multiple problems)  :: 53 of 200
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:34:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 682570
            Orig. end                         :: 692882
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LDHL01000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10312
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10312
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10312
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5312](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5312)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    384..400
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.8"
                     /tool="antismash"
     gene            complement(484..1368)
                     /locus_tag="ABD98_RS06320"
                     /old_locus_tag="ABD98_06270"
     CDS             complement(484..1368)
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526760.1"
                     /locus_tag="ABD98_RS06320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_06270"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_002031966.1"
                     /transl_table=11
                     /translation="MGNVEKMANFLLEEPRREQLFPLFEEVFGITSQTLQDFSEKGYWD
                     YTYKALSFLQDDNAIANVAAFSLPLLINGEKINATGIQSVMTHPNYRRRGLMKQLFSKM
                     IEEIDEQCECAVLFTENPELYKPFGFQIVQEYLMTVSYENKGYKDSQLKKLDFYNEEDR
                     QLIHEIIENGQRLSNKFSTLNYRSSFYLNMYDSTWNENLYYSEKLDALIVFTVENQKLK
                     LFGVFAPVIPILDELCEEISGEFTEIEFYFYPDQLGIDDVQYTELQTSKYLMVRSNKEL
                     DFKGYKFPVLTEF"
     gene            complement(1631..2320)
                     /locus_tag="ABD98_RS06325"
                     /old_locus_tag="ABD98_06275"
     CDS             complement(1631..2320)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002031964.1"
                     /locus_tag="ABD98_RS06325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_06275"
                     /product="MOSC domain-containing protein"
                     /protein_id="WP_002168398.1"
                     /transl_table=11
                     /translation="MTKVYEILSLNLGLPKEVMYGGKLIQTGINKKQVKEPVFLSFLKF
                     NGDGQADLVHHGGVDKAVCVYTGDHYPYWEKELNQELVYGAFGENITVSGMREEDVCIG
                     DTFQLGEAIVQVTQPRQPCFKLAKKYNIPKLPLYFQDTGYTGFYFRVLKEGWILPVDTL
                     KLLKLDPKGVSILFANRIMHKEKQNSEGLKRILEVNALSTSWRKTFEKRVKGEEISTKE
                     RLEGTKE"
     gene            complement(2335..3660)
                     /gene="gabT"
                     /locus_tag="ABD98_RS06330"
                     /old_locus_tag="ABD98_06280"
     CDS             complement(2335..3660)
                     /EC_number="2.6.1.19"
                     /GO_function="GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (34-374). E-value: 4.7e-122.
                     Score: 399.2. Matches aSDomain:
                     nrpspksdomains_ABD98_RS06330_Aminotran_3.1"
                     /codon_start=1
                     /gene="gabT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200502.1"
                     /locus_tag="ABD98_RS06330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_06280"
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_002031963.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.7e-132, bitscore:
                     437.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKSSVLPGSYSQVLQKRYEEAVPTGIYHLTPLYVKEARGAIITD
                     VDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAESFIHPCFHVTPYENYITLAEKINEK
                     VPGTSKKKTMFANSGAEAIENAVKIARKVTGRSKVISFERGFHGRTLMTMSLSTKETPY
                     KAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQVIYQFEKFLEEVSPEKVAAIVMEPV
                     QGEGGFIVPSISFVKRLREICNQYGILMIMDEVQTGFGRTGKWFATEHFHIEPDIMTLS
                     KSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGSPLSCAAGLAVFEIMEEENLVERAQQ
                     IGNRIMSHLHQMQLKYEMIGDVRGIGAMVAMELVTNRITKEPAKELTVKLIEDFWKNGL
                     ISIGAGIFDNVLRFLPPLVISNEEIDRGFEIINESFEKLCRK"
     aSDomain        complement(2539..3558)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ABD98_RS06330_Aminotran_3.1"
                     /evalue="4.70E-122"
                     /label="ABD98_RS06330_Aminotran_3.1"
                     /locus_tag="ABD98_RS06330"
                     /protein_end="374"
                     /protein_start="34"
                     /score="399.2"
                     /tool="antismash"
                     /translation="VKEARGAIITDVDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAE
                     SFIHPCFHVTPYENYITLAEKINEKVPGTSKKKTMFANSGAEAIENAVKIARKVTGRSK
                     VISFERGFHGRTLMTMSLSTKETPYKAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQ
                     VIYQFEKFLEEVSPEKVAAIVMEPVQGEGGFIVPSISFVKRLREICNQYGILMIMDEVQ
                     TGFGRTGKWFATEHFHIEPDIMTLSKSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGS
                     PLSCAAGLAVFEIMEEENLVERAQQIGNRIMSHLHQMQLKYEMIGDVRGIGAMVAMELV
                     "
     gene            3790..4692
                     /locus_tag="ABD98_RS06335"
                     /old_locus_tag="ABD98_06285"
     CDS             3790..4692
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003203974.1"
                     /locus_tag="ABD98_RS06335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_06285"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_087974007.1"
                     /transl_table=11
                     /translation="MISIELRQLEYFLAVSKELHFTKAAEKLNISQPSLSQQIRALEHE
                     VGMPVFDRIGKKISLTEAGRVLLSHSKTIFHEVEQARSAIQDLNGLHHGSLTIGSLLTV
                     VNYLLPPAILDFNKQYPNIELSVLGLRTGEIREKLLQNELDIGITFLPVQDKEIVSIPL
                     YKSELTLVVPTSHSLTERKYVSIEELQDYPLILLPKNFYLTELITSHCQNFNFKPKPIL
                     EISTMESLIQMVAQGMGITVLPKPYIDFLQNEHIQAIKIENPTPTIDIGLIYRRDKYMC
                     AATRKFIEQLKITVHSLQK"
     gene            complement(5001..5312)
                     /locus_tag="ABD98_RS06340"
                     /old_locus_tag="ABD98_06290"
     CDS             complement(5001..5312)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002189588.1"
                     /locus_tag="ABD98_RS06340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_06290"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_063226656.1"
                     /sec_met_domain="TIGR03601 (E-value: 1.8e-34, bitscore:
                     114.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNFQQELQALSLNDYQPGNIVYWDQQNQYPYYYITDDARRCGGC
                     GGGRCGGCGRCGGGRCGGFRCFGCFGCFSCFGCGGCSNCSNCSNCFDGFNGTTVTFEY"
     misc_feature    6008..6025
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.83"
                     /tool="antismash"
     gene            6256..6549
                     /locus_tag="ABD98_RS06345"
                     /old_locus_tag="ABD98_06295"
     CDS             6256..6549
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002168401.1"
                     /locus_tag="ABD98_RS06345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_06295"
                     /product="hypothetical protein"
                     /protein_id="WP_002127353.1"
                     /transl_table=11
                     /translation="MCNSKPIDELNIEDLKQNPIWEWAIDEAENEECDETWVKPVETIN
                     FTEELNGSIVLGELIIHNDEKFPMMCSIDIENNEVLISSIVFITKKKMSILL"
     gene            complement(6796..7839)
                     /locus_tag="ABD98_RS06350"
                     /old_locus_tag="ABD98_06300"
     CDS             complement(6796..7839)
                     /EC_number="1.2.1.11"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1219: N-acetyl-gamma-glutamyl-phosphate reductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200507.1"
                     /locus_tag="ABD98_RS06350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_06300"
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="WP_002127352.1"
                     /transl_table=11
                     /translation="MSEKSYHVAVVGATGAVGQKIIALLERVTKFNIEEITLLSSKRSA
                     GKIVQFKGREIKIQEAKSTSFEGVDIAFFSAGGEVSRQYVNHAVSSGAIVIDNTSEYRM
                     AHDVPLVVPEVNAHTLREHKRIIAVPNCSALQMVTALQPIRKSFGLERIIVSTYQAVSG
                     SGIHAIHELKEQAKSMLTGEEVKSTILPVKKDKKHYPIAFNVLPQVDIFTDNDFTFEEV
                     KMIQETKKILEDPNLKMAATCVRVPVVSGHSESVYIELEKEATVAEIKEVLLNAPGVIL
                     QDNPSEQIYPTPLYAEGKRDTFVGRIRKDPDTSKGFHLWIVSDNLLKGAAWNSVQIAET
                     LVGNGII"
     gene            7969..8841
                     /locus_tag="ABD98_RS06355"
                     /old_locus_tag="ABD98_06305"
     CDS             7969..8841
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016095293.1"
                     /locus_tag="ABD98_RS06355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_06305"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_002127351.1"
                     /transl_table=11
                     /translation="MEMRHVKTFCAIVKYGSFSKAAHALGYAQSTVTAHIKALENDLNI
                     PLFDRLGKKILLTKAGHQFHPYALELLTIYDKAQEIPQNSDHLEGTLAITSNESLAVYR
                     LPQLLRTYKQENPKVNIVLETNTNEQALKKLHEGETDVIFIIGESIEHNDFITCNLCNE
                     TFGWIFPAHFPSNTDPLLLLQDTQFIFTEQSCGYRPMVDRFLRQSGRNPNKTFETSNVE
                     VIKQSVMCELGISILPYIVVQESNQKEQLCFQPIETPAVIQSHVIYHKSRWISPVLQSF
                     LTLLERIEK"
     gene            complement(8947..9552)
                     /locus_tag="ABD98_RS06360"
                     /old_locus_tag="ABD98_06310"
     CDS             complement(8947..9552)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853587.1"
                     /locus_tag="ABD98_RS06360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_06310"
                     /product="PRK06770 family protein"
                     /protein_id="WP_002127350.1"
                     /transl_table=11
                     /translation="MKTLFKIIGIIAGMAVIGVGVTYGMLYYLNNSKPAAKKASPAAPA
                     VEVLADSNVKAEDAKILENGNYSLPNSGFNKNFKWTDENIQTALHEMAHQKAKADQKWG
                     YIFITQERIESLLEIINSNDVAQKSTYVDILERWKQGKYEKVDVDHNKIWKLQSGNLGE
                     GKGVMSEKEQKELINQVFIQKGTYSGSKLIAGEVQANK"
     misc_feature    10227..10243
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.99"
                     /tool="antismash"
ORIGIN
        1 cgttttcaca tatattgtat aatcgccaag ttttcgaata attcgaaaac gtaattgttg
       61 cttagcaaac tcatttggat agtcttgcgt tgaccaatca gaatagccat tattcgttac
      121 gacagcacct aaatgtgatg ggccctcagg aatatattct aaagatgttt ttaaccaaca
      181 cttctcagaa atcataacaa ataaacccgc ttgatcgtat gtcgcatttg gattcatgcg
      241 caattgaacc tcagcctgaa aatctgtcat tatctcatca taaaatacat ggccattcat
      301 tacttcaaaa ccataatggg ttttcagcca aaaatctgtt tcttctttcg aatggacaat
      361 gaatgtatca ttgtcttcaa gtttgtaaaa tttaggtgcg ttcattacag taagttgctc
      421 aatatgattt ggtatatatg atgttttcat aattcctcca taaaaaagca tttaaattaa
      481 tatttaaaac tcagttaata ccgggaattt ataaccttta aaatctaatt ctttattact
      541 tcgaaccatt aaatacttac tagtctgtaa ttctgtatat tgtacatcat caattcctaa
      601 ttgatctgga tagaaataaa attcaatctc cgtaaattcc ccagagattt cctcacataa
      661 ttcatctaaa atcggaatta ccggcgcaaa tactccaaat aattttagtt tttgattttc
      721 tactgtaaaa acaatcagcg catctaattt ctctgaataa taaaggtttt cattccatgt
      781 tgaatcatac atatttaaat aaaacgaaga gcggtaattt aacgttgaaa atttattaga
      841 aagcctttgg ccattttcaa taatctcatg tattaattgt ctatcctctt cattataaaa
      901 atctaacttt ttaagttgag aatctttgta gcctttattt tcgtatgata ctgtcatcaa
      961 atattcttgc acaatttgaa aaccaaatgg tttatatagt tccggatttt cagtaaacaa
     1021 gaccgcacac tcgcattgct catcaatttc ctctatcatc ttactaaaca attgcttcat
     1081 taatcctcgt ctacgataat tcggatgtgt cattactgat tgaatacccg ttgcatttat
     1141 tttttcacca ttaattaaaa gcgggagtga aaatgccgca acatttgcaa tcgcattatc
     1201 atcttgtaaa aatgataatg ctttatatgt ataatcccaa taccctttct ctgaaaaatc
     1261 ctgcaatgtt tggctcgtaa tcccaaacac ttcttcaaat aaaggaaata actgttctct
     1321 tctcggttct tctaatagaa agtttgccat tttttcaaca ttgcccatgt tgtccctcca
     1381 tacgtcgatt ccattaattt agatatactt atatgaccta ctctcaaaaa ataggccttt
     1441 caaatcaata tagtatcata aaacttttaa aaatggttta atttatttta tgttttgatt
     1501 attaaaaaac aagtcgaata aactagtatt aagatttttg aataatatca tgtttattat
     1561 ataacaaatg gtgcttccta cttatctttc cgataataga aatacaccat tttatgcaaa
     1621 tataattcac ctattctttg gttccctcaa gtcgctcctt cgtactaatt tcttctccct
     1681 tcactcgctt ttcaaacgtc tttctccaac tagtagacag tgcatttact tctaaaatcc
     1741 tttttaatcc ttcagagttt tgtttttctt tatgcattat gcgattcgca aacaaaattg
     1801 acacaccttt tggatcaagt ttcaacaatt ttaaagtatc taccggtaat atccaccctt
     1861 cttttaatac tcgaaaataa aaacctgtgt atcctgtatc ttgaaaatat aatggcaact
     1921 ttggaatatt atatttcttg gctaatttaa aacaaggttg tcttggctgc gttacttgta
     1981 caatcgcttc tccgagttga aacgtgtcac caatacaaac atcttcttca cgcattccgc
     2041 ttactgttat attttcaccg aaagccccat atacaagttc ctgattcaac tctttttccc
     2101 agtaaggata gtggtcacca gtataaacac aaaccgcttt atctacccct ccatgatgaa
     2161 ctaaatccgc ctgtccatct ccattaaact ttaaaaatga taaaaaaaca ggttccttta
     2221 cttgcttttt atttatccct gtttgaatta acttcccacc atacataacc tcttttggca
     2281 atcctagatt taaagatagg atttcatata ctttagtcat tcaaatccct cctcttactt
     2341 gcggcacaac ttttcaaaag actcatttat tatttcaaat cctctatcta tttcttcgtt
     2401 tgagataact agaggtggta aaaagcgtag tacattatca aatatccccg ctccaatact
     2461 tataagccca ttcttccaaa aatcttcaat tagctttact gttagttctt ttgcaggttc
     2521 tttcgttatg cgattcgtta caagttccat tgcaaccatc gcaccgattc cacgaacatc
     2581 accaatcatt tcgtacttta attgcatttg atgtagatgt gacattattc tatttccaat
     2641 ttgctgagcc ctttcaacta gattttcttc ttccattatt tcaaatacag caagaccggc
     2701 tgcacatgat aacggactcc cggcaaaagt tccaccaatt tgtcctctcc caggaatgtc
     2761 catcaactcc gcacgaccga caatagcact taatggtaaa ccgccagcaa ttgattttga
     2821 caaagtcatt atgtcaggtt ctatatgaaa atgttccgtt gcaaaccatt tccctgttcg
     2881 gccaaatcca gtttgcactt catccattat cattaaaata ccgtattgat tacaaatttc
     2941 tcgtaatcgt tttacaaatg aaatggaagg aacaataaac ccaccctctc cttgtactgg
     3001 ttccattacg atcgctgcta ctttttcagg tgatacttct tctaaaaact tttcaaactg
     3061 atagatgact tgagaatcca cttcttcagg agaaactcct tcttcagctc tgtaatagta
     3121 cggatacggc aatatatgca tgtctgaaac gaacggacca aatcctgctt tatatggagt
     3181 ttctttggtc gatagagaca ttgtcattaa tgttctccca tgaaatcctc gttcaaacga
     3241 aattacttta ctcctccctg ttactttacg tgcgatcttt acagcatttt caatcgcttc
     3301 cgcaccacta ttggcaaaca tcgttttttt cttacttgta cctggcactt tctcattaat
     3361 tttctccgct aaagtaatat aattttcata tggagttaca tgaaaacaag gatgtataaa
     3421 tgattctgct tgtttttgta ccgctcttac aactttcgga tgacaatgtc caacattttg
     3481 cataccaatt ccaccagcaa aatcaataaa ctgatttcca tctacatctg ttattatcgc
     3541 tccccttgct tcttttacat ataaaggcgt taaatgatat attcctgtcg gtacggcctc
     3601 ctcatacctt ttttgaagta cttgtgaata actgcccggt aatacactag atttcaacaa
     3661 ttcttcttca cctcatctaa ttttttaatc aatccatttc tgataaagtg attatatatc
     3721 ccacaaaacc attgttcaat tcaattattt atataataat gataggtata tcctatgatt
     3781 aaacttgagg tgatttcaat cgaattacga caattagaat actttttagc tgtttcgaaa
     3841 gaattacatt tcacaaaagc agccgagaaa ttaaatattt cacaaccttc actaagccaa
     3901 caaatacgtg cattagaaca tgaagtaggc atgccagttt tcgatcgaat tggtaaaaaa
     3961 atatctttaa ctgaagcagg aagagtttta ttatcgcaca gtaaaacaat ctttcacgaa
     4021 gtcgaacaag ctcgttctgc cattcaagat ttaaatggat tacatcatgg ttccttaaca
     4081 ataggatctt tgttaaccgt agtaaattat ttactaccac cagctatatt agatttcaat
     4141 aagcaatatc caaatatcga actctctgtg ttggggcttc gtacaggaga aattcgtgaa
     4201 aaattattac aaaatgaatt agatattggt attacatttc tcccggtgca agataaagag
     4261 attgtttcta tcccactata taaaagtgaa cttacattag ttgtgccaac cagccattct
     4321 ttaactgaac gtaagtatgt atctattgaa gaattacagg actatccttt aattcttcta
     4381 cctaaaaatt tctatttaac agaactaatt acatctcatt gccaaaactt taactttaaa
     4441 ccaaagccaa ttttagaaat tagtacaatg gaatctttaa ttcaaatggt cgcacaaggt
     4501 atggggatta ccgtgttacc aaaaccgtat atagactttt tgcaaaatga gcacatacaa
     4561 gctattaaaa ttgaaaaccc cactccaaca atagatatag gacttattta tagaagagat
     4621 aaatatatgt gcgctgctac ccggaaattt attgagcaac tcaaaataac agtacattct
     4681 ttacaaaaat aagcataaaa agtgattatc ctatgcagcc gttacacaac gacctattag
     4741 aataatctct ttttataatg atataaaaca ttacatttta tggtttcttt tctaataacc
     4801 tattattcat tataagtgct aaattattat cgtattatta agctacacat cagcgatatt
     4861 ttatgtctat ttcgtatagg tgttagcaag tccgtttgca aacttgttag cacattttta
     4921 taaaaattat ataatgcttc acaaagtagg aatccgtttc gatcaatctt ttttcaaaat
     4981 ggattccttt ttgtctctta tcaatattca aatgttacag tagtaccatt aaaaccatca
     5041 aaacaattag agcaattaga gcaattagag caaccaccac aaccaaaaca actaaagcaa
     5101 ccgaagcaac caaaacaacg gaatccacca caacgaccac cgccacaacg accacaacca
     5161 ccacaacgac caccgccaca gccgccacag cgacgagcat catctgtaat atagtaatat
     5221 ggatattggt tttgctggtc ccaatacaca atatttccag gctgataatc attaaggctt
     5281 aacgcttgta gttcttgttg aaaattattc atcttcatga cctccgttta taaactaaaa
     5341 ccccatcgat actttcctat atttctgttc gttaaatcta agtagaaata gcgctataaa
     5401 gtgaaacttt aatcagtagg ggttttgttc atcccccact gattattagc ccgcaccaat
     5461 cgggcgttta cgggcagcag ggctcccacc taacttcttt gctccagttg aattttgagg
     5521 tgggagtttt actgcccaca aatagcggga taaactaagt tcaatacgta caccaacaaa
     5581 atatgaccta tcactagatg atgcaccttg ttcatatgcc tatttttagg ttatttttac
     5641 tatgggctca tttttataaa gtgaaacttt aatcagaggg ggtttacggg cagttaatgc
     5701 gggctaaatg gacattataa aatatgaatt taaacaataa tatttagatt tatgtcatca
     5761 ttttctagtt ctatagtcgt atattccata attttgtttg gtcatcttcg tatttccaat
     5821 atgcactccc atctttattt ttcttcgctt caatatctgc tatatcagag ctaattttat
     5881 aagtatttat aattagtgat ttctgctaag aaaagtctat ttttatgaat ggcaaatcag
     5941 cgatgttttt tgctatttcc cccatcacaa ccacggtttt ccttttcaaa aggcattatt
     6001 tactgtctag ttaaaacctt gttaagatta ctgtaagaat agacttttaa taggttgtaa
     6061 aaataagttc aacattacaa aactagattt aataatatca aatggataat actctacatc
     6121 gaaacttctt atttgcgatc aactatttat tgaaacgagc aaacttgtat ttataggatt
     6181 gcaaatagcc acattttaca attaatgaat aaatttttta ctcatccatt ttcatggcaa
     6241 aaggagtata tattaatgtg taatagcaaa ccaattgatg aattaaatat tgaggacctg
     6301 aaacaaaacc caatatggga atgggcaata gatgaagcgg aaaatgaaga gtgcgatgaa
     6361 acgtgggtaa aaccagtgga aacaataaat tttacagaag aattaaacgg ttcaatagta
     6421 cttggcgaat taataataca taatgatgag aaattcccta tgatgtgtag tattgatatt
     6481 gaaaataatg aggtattaat tagttccatt gtttttataa cgaaaaagaa gatgagtata
     6541 ttgctataga agatgtagtg aaaaaggttg aaatgccttt atcacttaat attaatctca
     6601 caattaacgg agagcctaga tttttaaaat tctctgcaga taaagttgat atttataaaa
     6661 acactataaa aactaactta aattaaattt taattgaagt aagaggaaaa tactcattaa
     6721 gtaaaaaaca ggaaaccatt ataattaacg gtttcccaaa tatccttcta ttttcaatat
     6781 ttatataaat ccatactaaa taatcccatt tcccaccaat gtctctgcga tttgaactga
     6841 gttccaagca gcacctttta ataaattatc agaaacgatc caaagatgga atccttttga
     6901 tgtatcagga tctttacgta ttctacctac aaatgtatct cttttaccct ctgcatataa
     6961 tggcgttgga taaatttgtt cactaggatt atcttgtaaa ataacacctg gtgcattcag
     7021 tagtacttct ttaatttctg caacggtcgc ttctttttca agttcaatat aaactgattc
     7081 agaatgcccc gatacaactg gaactctgac gcaagtagct gccattttca gatttggatc
     7141 ttctaaaatt ttcttcgtct cttgaatcat ctttacttct tcgaatgtga aatcattgtc
     7201 tgtaaatata tccacttgcg gcaatacatt aaatgcaatt ggataatgtt ttttatcttt
     7261 tttcactggt aatatagtgc tcttcacttc ttcacctgta agcattgact tagcttgttc
     7321 ttttaattca tgaatcgcat gaattcctga gcctgatacg gcttgatacg ttgaaacaat
     7381 aattcgttct aaaccaaatg attttcgaat tggctgaaga gctgttacca tctgtagtgc
     7441 tgagcaattc ggaactgcaa tgatcctttt atgttccctt aaagtatgtg catttacttc
     7501 tggaacgacg agtggtacat cgtgtgccat tcggtattca cttgtgttgt caattacgat
     7561 tgcaccacta gaaactgcat gatttacata ttgtctagaa acttctcccc cagcgctaaa
     7621 aaaggcaatg tctacacctt caaaactagt tgattttgcc tcttgtattt taatctctcg
     7681 tcctttaaat tgtactatct taccagcaga tcgttttgac gagagtaatg taatttcttc
     7741 tatattaaat ttcgttactc tctctaacaa tgcaatgatt ttttgcccta ctgcgcctgt
     7801 agctccaact acagctacat gatagctctt ttcactcatt ttttctcctc caatttcaat
     7861 tgaatgaatt tctccacaac ttaattttaa aagaaattta atgatttgaa aaattgaaat
     7921 acatgatata atcattcaca aatcatgata ataaaagagg tatcatttat ggaaatgaga
     7981 catgttaaaa cattttgtgc cattgttaaa tatggtagct tttctaaagc tgctcatgca
     8041 ttaggttatg cgcaatctac tgtgacagcc catattaaag cattagagaa cgatttaaac
     8101 attcccctat tcgatcgatt aggaaaaaaa atacttctta caaaagcagg tcatcaattt
     8161 catccgtatg cattagaatt acttactatt tatgataaag cgcaagaaat ccctcaaaac
     8221 agtgaccatt tagaaggaac attggctatt acatctaatg aatcattggc agtgtatcgg
     8281 ttaccacaat tattacgtac ttacaaacag gaaaatccga aagtaaatat cgtacttgag
     8341 acaaatacca atgagcaagc attgaaaaaa ttacatgaag gagaaaccga tgttatcttc
     8401 ataattggag aaagtataga gcataatgat tttattacgt gcaatttatg taatgaaaca
     8461 tttggatgga ttttccctgc tcatttccct tcaaacactg atccactcct tttattacaa
     8521 gatactcaat ttatatttac agaacaaagc tgtggctata gaccaatggt agatcgtttt
     8581 ttacgtcaaa gcggaagaaa tcctaataaa acatttgaga cttccaatgt tgaagtcatt
     8641 aaacaatccg ttatgtgtga gttaggaatt tctattctcc cttacatcgt tgtacaagaa
     8701 agtaatcaaa aagaacaact ctgttttcag cccatcgaaa ctccagccgt cattcaaagc
     8761 cacgtaatct atcataaatc tagatggata tccccagttt tgcaatcgtt tcttacatta
     8821 ttagagagaa tagaaaagta agcgagtaat tacaaaaatg agtaactgct ctaaaaagca
     8881 attactcatt tttgttaaaa catttgtgtt cataacaata caggtgtttc aaaatcaata
     8941 tgtatattat ttattagctt gtacctcacc tgcaattaat ttactacctg aatatgtacc
     9001 tttttgtata aaaacttgat taattaattc tttttgctcc ttttctgaca ttactccttt
     9061 cccttctcct aaattcccac tttgtaattt ccaaatcttg ttgtggtcca catcaacctt
     9121 ttcatatttt ccttgtttcc atcgttctaa aatatccaca tatgtacttt tttgtgcaac
     9181 atcattacta tttataattt caagtaaact ttcaatacgt tcttgtgtaa taaaaatata
     9241 gccccatttt tgatcagctt tcgctttttg atgtgccatt tcatgtaatg ctgtctgtat
     9301 gttctcatct gtccatttga agtttttatt aaaaccacta ttcggcaatg aataatttcc
     9361 attttctaat atctttgcat cttcggcttt tacattacta tcagctagca cttctactgc
     9421 cggagcggct ggtgatgcct ttttcgcagc tggtttacta ttattcagat aatacagcat
     9481 accatacgtt acacctaccc cgattaccgc cataccagct ataattccta tgattttaaa
     9541 taatgttttc atcttgcttg tttcctccca aatatctctg ttcttactac ctcattattt
     9601 cttatataaa acagttcctt cattcaaaat agttcatccc atcccctatc atattattta
     9661 tatatatttc cttatattgt atttcttttt tcgtgaaatt gaaagattat tctgttaaaa
     9721 aataaagata ttctacaaaa ttttccttta tatttatatg gaaacaaaaa aacaacgagc
     9781 gccttctaaa acactcgttg tttttacttt atgcacgatt ttcttttaac cactccgtaa
     9841 tagcatctaa cctcgaaaca tcaaaacgtc tttcttcaca tacttttaag ctgtttaaac
     9901 ttttcacaat ttctaattca ttcgacttta tactttttgc ttcaatcaat ttacagtacc
     9961 cgtctgtgac ggtattgaga ttttcataat aacgctgaaa tgctttagat atatgtaaat
    10021 gtttttcaaa gtttcttctt tttttcgtcc catcaaactc tttccgtttt ctaagctctt
    10081 catcacttgt atataaataa aaatattgat ctggaaaacc aatttctcta tttatcatct
    10141 tttctttata aaagttttct attaaagata acggctgatt aaatatatca aaacgaaaac
    10201 accaattata actaagtggc tgatatatat ccccatcgag tattactaat tcatattgct
    10261 gagacttctg tattgctatt ttccagcgct ctacttgttt ttcaaaatac ca
//
