LOCUS       NZ_LDHL01000015        10270 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Bacillus mycoides strain G25-104 G25-104_contig000015, whole genome
            shotgun sequence.
ACCESSION   NZ_LDHL01000015
VERSION     NZ_LDHL01000015.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 1940029)
  AUTHORS   Zheng,Z., Zheng,J., Zhang,Z., Peng,D. and Sun,M.
  TITLE     Nematicidal spore-forming Bacilli share similar virulence factors
            and mechanisms
  JOURNAL   Sci Rep 6, 31341 (2016)
   PUBMED   27539267
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940029)
  AUTHORS   Zheng,Z. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2015) Microbiology, Institute of Life Sciences and
            Technology, Shizishan str., Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.3.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 170.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015845385.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 01:09:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,771
            CDSs (total)                      :: 5,604
            Genes (coding)                    :: 5,404
            CDSs (with protein)               :: 5,404
            Genes (RNA)                       :: 167
            rRNAs                             :: 14, 16, 15 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 16, 15 (5S, 16S, 23S)
            tRNAs                             :: 117
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 85 of 200
            Pseudo Genes (incomplete)         :: 137 of 200
            Pseudo Genes (internal stop)      :: 43 of 200
            Pseudo Genes (multiple problems)  :: 53 of 200
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:34:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 417715
            Orig. end                         :: 427985
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LDHL01000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10270
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10270
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10270
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5270](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5270
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            585..1481
                     /locus_tag="ABD98_RS05130"
                     /old_locus_tag="ABD98_05085"
     CDS             585..1481
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136580.1"
                     /locus_tag="ABD98_RS05130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_05085"
                     /product="DMT family transporter"
                     /protein_id="WP_197236022.1"
                     /transl_table=11
                     /translation="MHNKKWDFRIVCAHAFTILIWGTAFPGIRMGLEAYTPEHLTLLRL
                     LIASLILFLFSLIYKLRLPDLKDIPVIFIFGALGFTIYHIALNYGEKSVNAGPASLIVS
                     VTPILTAILASVFLNERMKLNGWIGGVISFVGIAFLSLSQGDSIQLNSGGLLILLAAIS
                     ESLFFVFQRPYLKKYGFLPFTIYTIWSGTVFMLIFLPGIYQEIVTAPIEITLSVIYLGV
                     FPTVLPYITSHASASEATSSLYLTPVTACIIAWIWLGEVPTFVSIIGGGITILGVVIAH
                     LTFNKDDYKQIESEKVW"
     misc_feature    complement(1499..1515)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.88"
                     /tool="antismash"
     gene            1765..2688
                     /locus_tag="ABD98_RS05135"
                     /old_locus_tag="ABD98_05090"
     CDS             1765..2688
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="ABD98_RS05135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_05090"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_002127616.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATGPLTNEITTYYPEIHGPEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGEDLTIVDVGRSTSLAIAFNLWNELMLNIKGIYFMGGVFLEAGNVTPLA
                     EANVYGDPIASQIVFQQAKNITLFPLNVTNKTVLTPSVFTYILVNTQNPFKSIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVLAISGLVNPTFLNYTSRKVTVDICGETKGQTFADFRPH
                     SKSEGARIALQLDEEKFIQDFMKIML"
     gene            2766..3230
                     /locus_tag="ABD98_RS05140"
                     /old_locus_tag="ABD98_05095"
     CDS             2766..3230
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136577.1"
                     /locus_tag="ABD98_RS05140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_05095"
                     /product="N-acetyltransferase"
                     /protein_id="WP_002168287.1"
                     /transl_table=11
                     /translation="MNVQLKIVTRENWEDALKLRVKENQIHLVPAVAVSLAKVYIKPDG
                     ENVEYIPFAIYDGDLIVGFIMHAFVRESTDMYWINGFIIDEKEQGKGYGKAALQEIINL
                     IKNNFKECEEIRLTVHKDNISAKKLYECYDFKPLGNVYDGEEVYRLLLLK"
     gene            3414..3983
                     /locus_tag="ABD98_RS05145"
                     /old_locus_tag="ABD98_05100"
     CDS             3414..3983
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002065694.1"
                     /locus_tag="ABD98_RS05145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_05100"
                     /product="DUF4256 domain-containing protein"
                     /protein_id="WP_063226187.1"
                     /transl_table=11
                     /translation="MKESNKKELSLEQREELLQTLKDRFENNMNRHKGLEWANVQERLD
                     ANTEKLWSLNEMERTGGEPDVVDFGKEKGEYIFYDCSAESPKGRRSVCYDLEALESRKK
                     HKPENNAIDMATAMGIELLTEEEYRTLHNLENFDVKTSSWVQTPSDIRELGGALFCDYR
                     FGHVFVYHNGAESYYAARGFRGSLRV"
     gene            4356..4801
                     /locus_tag="ABD98_RS05150"
                     /old_locus_tag="ABD98_05105"
                     /pseudo=""
     CDS             4356..4801
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="ABD98_RS05150"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_05105"
                     /product="NUDIX domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKSKFHHIVRAIMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVTYSIKQFLGVIENQWQAKETLHHEINHIFEIDSNELKSDFTPISKESH
                     LAFHWIDFNKENINSYTIMPTPSVKELLEKN"
     misc_feature    complement(4972..4987)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 26.71"
                     /tool="antismash"
     gene            5001..5270
                     /locus_tag="ABD98_RS05155"
                     /old_locus_tag="ABD98_05110"
     CDS             5001..5270
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001869.1"
                     /locus_tag="ABD98_RS05155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_05110"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_078203291.1"
                     /sec_met_domain="TIGR03601 (E-value: 7e-43, bitscore:
                     141.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEFQQELQALNLNAYQPGNVVYWDQQQNQYPYYYIQDDARRCGG
                     CGGRCGGCGGRCGGCGGGRCGGCVGCVGCVGCFSCSNCWNWWFI"
     gene            5384..6412
                     /locus_tag="ABD98_RS05160"
                     /old_locus_tag="ABD98_05115"
     CDS             5384..6412
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002085304.1"
                     /locus_tag="ABD98_RS05160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_05115"
                     /product="serine hydrolase"
                     /protein_id="WP_016127063.1"
                     /transl_table=11
                     /translation="MYKYEKLISWVESIKETNKSSAAALCIIKDNKIVLEHYSGTHSNT
                     PTSKKVTISSQFNVASARKSYLGLMVAYAVYEGKIKSLDDEAVNYFKEYDPSLLGKTTI
                     RHLVTHSHGLNETDDGTAFREFEPGQAWAYRDINVRMVTQLIYQLYNKSFPELLKERVF
                     TPADFKKTGWRIKQDDNLVKVVVNPDEDAISSVGAVDDGSEKNLFVSAREFAFWGNLHL
                     NKGFLNGKQIVPKEVIALATSLQSPEYTNKELPQNGLFWFVQNEPALFSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSSSKNTMV
                     RV"
     gene            6444..6587
                     /locus_tag="ABD98_RS05165"
                     /old_locus_tag="ABD98_05120"
     CDS             6444..6587
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="ABD98_RS05165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_05120"
                     /product="hypothetical protein"
                     /protein_id="WP_002013225.1"
                     /transl_table=11
                     /translation="MKNEVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFEEI
                     EY"
     gene            6866..8074
                     /locus_tag="ABD98_RS05170"
                     /old_locus_tag="ABD98_05125"
     CDS             6866..8074
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="ABD98_RS05170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_05125"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_002127613.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAGKLQQKFGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLINSLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNTATETLHNDYTPREMMKTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTIGRIILGTLSDKVGRLKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLCVISFVISIVIRPPNAEKKKEIKSLNR
                     KIA"
     gene            8255..8671
                     /locus_tag="ABD98_RS05175"
                     /old_locus_tag="ABD98_05130"
     CDS             8255..8671
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="ABD98_RS05175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_05130"
                     /product="N-acetyltransferase"
                     /protein_id="WP_002085307.1"
                     /transl_table=11
                     /translation="MQIVKQWVQEDSDYIREKVIEYNQKQLSNEEKTPSEKISFIVKNE
                     KEEIVGGITAITFWHHVYVDFLWVSEEYRHEGYGSNLMNLIEEFAIKKECRLIKLDTFS
                     FQAPNFYKKHGYKVIGVSEDYPKGHNHYYLEKRL"
     gene            8856..9299
                     /locus_tag="ABD98_RS05180"
                     /old_locus_tag="ABD98_05135"
     CDS             8856..9299
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="ABD98_RS05180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABD98_05135"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002085308.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDETHEKLLMVKNKGENGSYYTLPGGAVKFGETL
                     EEAAIREVKEETGLDISVKGVCSISEAFFEERGHHAIFFNFLGEITGGEICISRPKEIE
                     EITWMELDSAEPYLRIPEHLKSLLQKKETVPYIFNGTIIHQSS"
     misc_feature    complement(10238..10252)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.81"
                     /tool="antismash"
ORIGIN
        1 ttcctcacat acgttacgca taatgaattg catagtttct aacgggaaaa gatgaggtgc
       61 aagttctcct ttactaagat gaatcaattt tttatttgct tctgcttcat ttatttctcg
      121 taccattaac ttgcttggtt tatgaatacc agcctttaca tattcactcc aatcgggcgg
      181 gggattggtg gctaataatg accatgtatt attcgttaca attgttcctg acccgatttt
      241 cccttgaatt aagccatctg ccataagttc ttctagtgca attattacag tactacgatt
      301 tacatgaaat aatttcgcta actgccgctg gctaggaacc ttacttccaa tcggccattc
      361 tccgtattca attttttctt tcatatagtc tattatctgc cgatatttag gcagtttttt
      421 atcagtagac atattaaaaa ctccctcatt cattttaaaa atgcaaagtg gttggttttt
      481 tcgaggatta actggttgaa gacattgtat caactattaa atatgatgaa aagaaataat
      541 tggtaattag aaaatggctg gtttattgga gagggagaga atatatgcat aataaaaaat
      601 gggattttcg tattgtatgt gctcacgctt ttacgattct tatatgggga acagcttttc
      661 caggaattcg gatggggctt gaagcttaca cccctgaaca ccttactttg ctacgattat
      721 taatcgcttc tttgattctt tttctgtttt cacttatata caagttacgt ttacctgatt
      781 tgaaagatat tccagtaatt tttatatttg gtgctcttgg atttacaatt tatcatattg
      841 cactgaacta cggtgaaaag agtgtgaatg ctggacctgc tagtttaatt gtatcagtaa
      901 cacctatatt aacggcaatt cttgcttctg tttttttgaa tgaaagaatg aaattaaatg
      961 gttggatcgg tggtgtaatt agttttgtag gaattgcttt tttatcgtta agtcaaggag
     1021 attccattca attgaatagt gggggattac taatattatt ggcggcaatt tcagaaagtc
     1081 tatttttcgt ttttcaaagg ccatacttaa aaaagtacgg tttcttgcca tttacaatat
     1141 atacaatttg gtctggtact gtatttatgc ttattttttt gccagggatt tatcaagaaa
     1201 tcgtaacagc tcctatagaa attactttga gtgttatata tttaggtgtg ttcccgacag
     1261 tactgccgta tattacatcc catgccagtg cttctgaggc aacaagttct ctatatttaa
     1321 caccagtaac tgcatgtatt atcgcttgga tatggttagg tgaggtgcca acctttgttt
     1381 cgataattgg agggggaatt actatacttg gagttgtaat tgctcattta acatttaata
     1441 aagatgatta taagcaaatt gagagtgaaa aggtatggta agacatccct acgagtattt
     1501 gcaatttgaa tgcaaaattt cggattaata acaggttttc aattctataa atatagtgaa
     1561 aaaaatctat attaaaatca gttagtccaa tatttgtacg agatttaatt aaactggtag
     1621 attatttatc tgcaaaagaa ctagcataat gctagttctt tgttatcaat acattactca
     1681 ccattaatat ttgcttagaa ctgtatagat ttaatttttt tccacatata cataattatc
     1741 ttacctatga aaggagtgca tttcttggag aaggttttat tttttggaga cccaggtatt
     1801 gatgattcgt ttgcaattat gtatggctta ctgcatcctg aaattgaaat tgtgggtatc
     1861 gtaactggat atggaaatgt tgaacatata catgccgctc ataatgccgc atatatttta
     1921 cagttagcaa acagacaaga tattcctgta attagcggtg caacaggccc acttacaaat
     1981 gaaattacaa cgtattaccc tgaaatccat ggaccagaag gattagggcc catccatgta
     2041 ccggaaagtg tgttatctat tcctatatac aatttcggta gtattgctgc gattcttgta
     2101 aagtatggag aagatttaac aattgtcgat gtaggaagat ctacctcgtt agcgattgct
     2161 tttaatttat ggaacgaatt gatgttaaat ataaaaggaa tttattttat gggtggtgtc
     2221 tttttagaag cagggaatgt tactccatta gcagaggcaa acgtgtacgg agatcctata
     2281 gcaagtcaaa ttgtatttca gcaagcgaaa aatataacgt tattcccttt aaatgtaaca
     2341 aataaaacgg ttttaacccc aagtgtattt acttacattt tagttaatac acaaaatcca
     2401 tttaagtcga ttatgaaacc catgtacgat tattatttat cagcatacca aaaactcaat
     2461 ccatctattg aagggccatt attacacgat gtacttgcta taagcggttt agtgaatcct
     2521 acatttttaa attatacatc ccgaaaagtg actgtggata tatgtggcga aacgaaggga
     2581 caaacttttg ctgattttcg cccacactct aaatctgaag gggcgcgtat agcgttacaa
     2641 ttagatgagg aaaaatttat tcaggatttt atgaaaatca tgttataagt tgatgtcgaa
     2701 aaagggaatc atttcactcc gttgaatata aaagataaat tctcttttta aaggagctta
     2761 aaatagtgaa cgttcaatta aaaattgtta cgagagaaaa ttgggaagac gcattaaagt
     2821 tacgagttaa agaaaatcaa atccatcttg ttcccgcagt ggcagtatca cttgctaaag
     2881 tatacataaa accagatggt gaaaatgtcg aatatattcc attcgctata tatgatggtg
     2941 accttatagt tggttttatt atgcatgctt ttgtacgaga gtcaacagat atgtattgga
     3001 ttaacggatt tatcattgat gaaaaagagc aaggaaaagg atatggaaaa gcagcattac
     3061 aggaaatcat taacttaata aaaaataact ttaaagagtg tgaggaaatt agattaactg
     3121 tccataaaga taatatttct gcaaagaaac tatatgaatg ttatgatttt aaaccattag
     3181 gaaatgtata tgacggtgag gaagtatatc gcttattgtt gttgaaataa agatgtactg
     3241 ttttgaaaca ataagttgtt ccgttaaagg gtcatattgc ggagaattgg ttgtactaat
     3301 tcatataaat tatcgcaaac tcaattcacg caattattta aacattttat tataaaacag
     3361 tatttatata tacttaaact atcattaata aaaatatcaa tggaggaact aatatgaaag
     3421 aaagcaataa aaaagagttg tcactagagc aacgtgaaga attactacaa acattgaaag
     3481 atcgttttga gaataatatg aaccgtcata aaggtttaga atgggctaat gttcaagaaa
     3541 ggctggatgc taatactgaa aagttgtggt cgctcaacga aatggaacga actggtggcg
     3601 aacctgatgt tgttgatttt ggtaaagaaa aaggcgaata tattttttat gattgttcag
     3661 cagaaagtcc taaaggtcgt agaagtgttt gttacgatct tgaagcgcta gaatcaagaa
     3721 agaaacataa accagaaaat aacgctattg atatggcaac tgccatgggt attgaactat
     3781 taacggaaga agaatatcgt acattacata atcttgaaaa tttcgatgtg aagacgtcga
     3841 gttgggtgca aacaccttct gatattagag aacttggcgg tgcccttttt tgcgattatc
     3901 gctttggaca tgtctttgtg tatcacaatg gagcagaatc ctactatgcc gcaagaggtt
     3961 ttcgtggttc gttaagagtc taaaatttgt acaggcaggt aaatttgaag tagagaatag
     4021 atttgcttag ggaatagtag tgattgcata ctttagctac ggcttcgatt ttgaggccgc
     4081 aaagaatagg gctaatcaaa ccttattgta aaagaatgaa tcaactaaaa atattaagag
     4141 tatgttttga taattttctt cataaacata ctctttttta gaatctttta tcaaaattat
     4201 tagttttaat taacgtataa acgagaactt gcaaaaggta ctcttgcaat tatcaatgaa
     4261 attcacccat atgaaacacc tatgatttat attatcccgc ttttaaatga aaattttcga
     4321 gcaagtataa agtgaatgat taggaggaat acaaaatgaa aagtaaattc catcatattg
     4381 tacgagctat catgataaaa gatgaaaaat tactagttgc tgaatacata ggccatcact
     4441 attttttacc cggtggtcat gttgaaattg gggaatcagc agagaatgca ctaataagag
     4501 agttacgaga agaacttgga gtaacttaca gtataaaaca atttttaggc gtcatagaaa
     4561 atcaatggca agccaaagaa accctccacc atgaaattaa ccacattttt gaaatagatt
     4621 caaatgagtt aaaatccgat ttcactccaa tatctaaaga gtcccattta gcatttcact
     4681 ggattgattt taataaagaa aatataaatt cctatacaat aatgccgaca ccttcagtaa
     4741 aagaattact agaaaaaaat taagtgatga actattaact tgctggatta gtaattttta
     4801 attgtctcta tttatattaa ttccgtccac taaaagaata taaagaaaat aggctcataa
     4861 acaaggtaca tcacctagtg taaagtcata ttttgttgat gtacgcattt agtctatagc
     4921 cttatttcta ctttattttt aaaagtaaat aaactcaaaa atttggacgg aatttacatt
     4981 tgtaaatgga gggtttcata atgaatgagt ttcaacaaga actacaagcg ttaaacctta
     5041 atgcttatca acctggtaat gttgtgtatt gggatcagca acaaaatcaa tatccatatt
     5101 actacattca agacgatgca cgtcgttgtg gcggatgcgg tggacgttgt ggcggttgtg
     5161 gtggtcgctg tggtggttgt ggcggcggac gttgtggcgg ttgcgtaggt tgtgtaggct
     5221 gtgttggttg tttcagctgt tctaattgct ggaactggtg gtttatttaa gtattataga
     5281 tctataatat ttcacatgaa agaaaagtgc agatgcaaag ttataatgta ttgaaacttt
     5341 tctttcatgc tgttattttt atatgtttta gggggatatt tatttgtaca aatatgaaaa
     5401 attaatttca tgggtagaaa gcattaaaga gaccaataaa agttctgcgg cagcactttg
     5461 tattattaaa gataataaga ttgtactaga gcattatagt ggaacgcatt caaatactcc
     5521 tactagtaaa aaagtaacta tatcttcaca attcaatgtc gcttccgcaa gaaaaagcta
     5581 tttaggatta atggtagctt atgcagttta tgaggggaaa ataaaatctc ttgatgacga
     5641 ggctgtaaat tattttaaag aatatgatcc ttcattactt ggaaagacaa cgataagaca
     5701 tttagtaact cattcacacg gtttaaatga aaccgatgat gggacagctt ttcgtgaatt
     5761 tgagccggga caagcttggg cgtacagaga tattaatgta agaatggtga cgcagcttat
     5821 ttatcaactg tataataaga gttttcctga actattaaag gagcgtgtat tcacacctgc
     5881 tgatttcaaa aaaactggat ggagaataaa acaagatgat aatttagtta aggttgttgt
     5941 aaacccagat gaagatgcaa taagtagtgt tggtgcagta gatgacggct ccgagaaaaa
     6001 tctttttgtc tctgcgagag aatttgcatt ttggggtaac cttcatttaa ataaagggtt
     6061 tttaaatggt aaacaaattg tgccaaaaga agttatagca cttgctacga gcttacaaag
     6121 tccagagtat acaaataaag aactacctca aaacgggttg ttttggttcg ttcaaaatga
     6181 acctgcttta tttagcgaac ttggtgagcg agttcctaaa ggttcctatc aaatattagg
     6241 aattacagga ccaacgatat tagtcattcc agaatataat gtcgtcgttg ctaaaatgta
     6301 caataaaaga tacaactatg gtggggataa ttatttatat tatttacgtg aatttagcaa
     6361 tttagtcgct gatacattta gtagcagtaa aaatactatg gttagggtat aactactcct
     6421 ataataaaaa ggagtgaact ttaatgaaaa atgaagtgaa taaaaataaa aagcaaaata
     6481 taaaggatgt aaacacagaa caaaacgcga tttatagcga tccgaaagat gcagctaata
     6541 tgcaaactgt accgcagccg aaagactttg aggaaattga atattaattt tctctccgac
     6601 ttatttatga aagaaaaata cgtcttaagt ctcatcactt tcagcaaatt atttgataac
     6661 atatatacat aagcattcga aaatgtctta tcttaacaaa gctttcttta aatgctgtat
     6721 ataacctaca aaaaggcact tcttgatgaa gtgtcttttt gtgtaagcta actcaatttc
     6781 cattttccgt acagtatctt gcaatttatc atggatatac taactgtgaa aattcatgtc
     6841 atgataaaag aaggggacag ttagcatgaa acaaacatct ataaatccat tgctaattgt
     6901 tctaggtaca atcattgttc aaattggcct aggaacaatt tacacatgga gtttatttaa
     6961 tcaacccctt gtaagtaagt ttggatggaa cctcaattca gtcgcgatca cgttctcaat
     7021 tacgagtttt tctttatcat tctcaactct atttgcagga aagctacaac aaaaatttgg
     7081 acttcgtaaa cttatcgcta ctgcaggaat tgttttagga ctcggtttaa tacttagttc
     7141 acaagtttcc tcattaccat tactttattt attagccggt gttgtcgttg gatacgctga
     7201 tggaacagca tatattacat cactatctaa tttaattaaa tggtttccaa accgtaaagg
     7261 acttatttct ggtatatctg tttcggcata tggaatggga agcttaatct ttagatatat
     7321 aaacggaaat cttatcaaca gtcttggagt atcacaagca tttttatatt ggggtattat
     7381 cgtcttacta ttagtgttga taggatcatt cttcttacgt gaagcaattg taagtaacac
     7441 tgcaactgaa acattacaca atgattatac gccgcgtgaa atgatgaaaa cgaaacaagt
     7501 atatctctta tttttcatgt tattcacatc atgtatgggc ggactgtatt taatcggtat
     7561 ggtaaaagac atcggggtgc agctcgttgg tcttagtgca gcgacggccg ctaatgccgt
     7621 cgcaatgatt gcaatcttta atacaatagg tagaattatt cttggaacgt tatcagataa
     7681 agtaggccga ttaaaaatcg tctcggctac atttattatt attggattat ccgtcttcac
     7741 tttaagtttt attccactaa attacggaat ttattttgct tgtgtagcaa gtgttgcctt
     7801 ttgctttggt ggtaacatca ctatcttccc agcaattgtg ggagacttct tcggattaaa
     7861 aaatcatagt acaaactacg ggattgttta ccaaggtttc ggatttggtg cacttgcagg
     7921 atcattcatt ggagcactac ttggcggatt tcagccaacc ttcattacaa tcggtgttct
     7981 atgtgttata tctttcgtta tctctatagt aatccgccct ccaaatgcag aaaagaagaa
     8041 ggaaatcaaa tcgttaaatc gaaaaatagc ttaatgctaa gaaagagtcc ttatactagg
     8101 gctcttttgc tttacaaaaa aatgtataaa caatgaatga cattcaaaaa atctaatttt
     8161 ttccctgtac ttatttcata atctaacatt aaagaagata ggaaattccg attattaaat
     8221 tgaataaata atgtaaaggg gcgtgatagt taaaatgcaa atagtaaagc aatgggtaca
     8281 agaagatagt gattacataa gagaaaaagt aattgaatat aaccaaaaac aactttcaaa
     8341 tgaagaaaaa acaccttcag aaaagattag ttttatagtg aagaatgaaa aagaagaaat
     8401 agtaggaggg ataacggcaa tcacattttg gcaccatgta tatgttgact tcctatgggt
     8461 ttctgaagaa tacaggcatg aaggttatgg aagcaattta atgaatctta ttgaagaatt
     8521 tgcaataaaa aaagaatgta ggctaatcaa attagatact tttagtttcc aagcccctaa
     8581 tttttataag aagcatggat ataaagtgat aggggtaagt gaagattatc ctaaagggca
     8641 taatcattac tatttagaaa aaaggttata aatgaatcat taaagagtgt attatgatta
     8701 atctttttca aaatatgctc ttttcttttg tgtgtctatg aacacgatat cgtttcgtat
     8761 tagttattta ctcaaattac tttttatgaa tcaaaacaat ttaatgatag aataaaataa
     8821 agacagaaaa ttttcaccat tcaagggggt tacatatgaa aaaagtaaat gttacatacg
     8881 cacttctata tgatgaaact catgaaaagc tattaatggt taaaaacaaa ggggaaaatg
     8941 gttcttatta cactttacca ggtggagcag ttaaattcgg ggaaacgtta gaagaggcag
     9001 caattcgtga agtgaaagaa gaaacagggc tagacatatc cgtaaaaggg gtttgttcca
     9061 ttagtgaagc cttttttgag gagagaggac atcatgcaat cttctttaat tttttaggag
     9121 aaataaccgg aggggaaatc tgtatatcac ggccaaaaga gattgaggag attacctgga
     9181 tggaattaga ttcagcagaa ccttatttac gtataccaga acacttaaaa agccttttac
     9241 aaaagaaaga aacagttcct tatattttta atggaactat tattcatcaa tcttcataaa
     9301 gatagtattt catttaaggc gggggaaaaa tggagtttat cgttaatcac aaacaattta
     9361 cacaagctct ttcagaagta agtaaagcta tctcaacaaa aactttaatt cctatattat
     9421 ccggaataaa gataacagca gatcaatctg gaattacttt aatcgcaagt aattcgaata
     9481 ttttcattga aaaatttata cctatttcaa ttgaagacga acaaattaca actattttaa
     9541 aagcaggaag tattgttgta ccagcaaagt atttcattga aattattaag aaaatgccaa
     9601 gtgagatttt aataaaaagt atgaatgagc agttgattac aattcaatca gatgaaatta
     9661 ctttaaactt aaatggactc tcggcaaatg aatttcctaa tgtaccattt ttagactcac
     9721 atacagaaat acaagtagaa acggaacagc tgattgaggt attcaaacaa actgtctttg
     9781 caactgctaa aaatgaatct agaccagttc ttactggcgt acatttcgtt attgatcata
     9841 acaaattaat ttgtgctgct actgattcgc acagactggc tttacgtgaa atacccattt
     9901 cttcacatgc gaaattaaat tgcattgtac caagttccac tattagtgaa ctactaaaat
     9961 taatgagcag taattcaaat ttagtataca tatatctttc agagagtcac attattttta
    10021 aattcggtac aattacgttg tattcaaggc ttattgaagg gaaatatcct aatatatccg
    10081 gcctaattcc aaatgaatcc caaacgatta ttaatataga tagaaaaaag attttacaag
    10141 gtgtagatcg gtcaagttta ttggctagtg aatgggcaaa caacaacgtt aacttagaaa
    10201 tcatcgacga atgcacaata caaatttctt ccaacgcttc tcaaattggt aaaatatccg
    10261 agacacaaca
//
