LOCUS       NZ_LDGT01000039        10276 bp    DNA     linear   CON 30-AUG-2024
DEFINITION  Bacillus mycoides strain G25-84 G25-84_contig000039, whole genome
            shotgun sequence.
ACCESSION   NZ_LDGT01000039
VERSION     NZ_LDGT01000039.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 352559)
  AUTHORS   Zheng,Z., Zheng,J., Zhang,Z., Peng,D. and Sun,M.
  TITLE     Nematicidal spore-forming Bacilli share similar virulence factors
            and mechanisms
  JOURNAL   Sci Rep 6, 31341 (2016)
   PUBMED   27539267
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352559)
  AUTHORS   Zheng,Z. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2015) Microbiology, Institute of Life Sciences and
            Technology, Shizishan str., Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.3.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 170.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015845055.1-RS_2024_08_30
            Annotation Date                   :: 08/30/2024 03:02:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,776
            CDSs (total)                      :: 5,620
            Genes (coding)                    :: 5,416
            CDSs (with protein)               :: 5,416
            Genes (RNA)                       :: 156
            rRNAs                             :: 13, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 1 (5S, 16S)
            partial rRNAs                     :: 13, 14 (16S, 23S)
            tRNAs                             :: 110
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 79 of 204
            Pseudo Genes (incomplete)         :: 129 of 204
            Pseudo Genes (internal stop)      :: 54 of 204
            Pseudo Genes (multiple problems)  :: 50 of 204
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:03:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 301811
            Orig. end                         :: 312087
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LDGT01000039.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10276
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10276
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10276
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5276](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5276)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    19..33
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.81"
                     /tool="antismash"
     gene            complement(972..1415)
                     /locus_tag="ABE17_RS17625"
                     /old_locus_tag="ABE17_17295"
     CDS             complement(972..1415)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="ABE17_RS17625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_17295"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002085308.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDETHEKLLMVKNKGENGSYYTLPGGAVKFGETL
                     EEAAIREVKEETGLDISVKGVCSISEAFFEERGHHAIFFNFLGEITGGEICISRPKEIE
                     EITWMELDSAEPYLRIPEHLKSLLQKKETVPYIFNGTIIHQSS"
     gene            complement(1600..2016)
                     /locus_tag="ABE17_RS17630"
                     /old_locus_tag="ABE17_17300"
     CDS             complement(1600..2016)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="ABE17_RS17630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_17300"
                     /product="N-acetyltransferase"
                     /protein_id="WP_002085307.1"
                     /transl_table=11
                     /translation="MQIVKQWVQEDSDYIREKVIEYNQKQLSNEEKTPSEKISFIVKNE
                     KEEIVGGITAITFWHHVYVDFLWVSEEYRHEGYGSNLMNLIEEFAIKKECRLIKLDTFS
                     FQAPNFYKKHGYKVIGVSEDYPKGHNHYYLEKRL"
     gene            complement(2197..3405)
                     /locus_tag="ABE17_RS17635"
                     /old_locus_tag="ABE17_17305"
     CDS             complement(2197..3405)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="ABE17_RS17635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_17305"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_002032385.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAGKLQQKFGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLINSLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNTATETLHNDYTPREMMKTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTIGRIILGTLSDKIGRLKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLCVISFVISIVIRPPNAEKKKEIKSLNR
                     KIA"
     gene            complement(3684..3827)
                     /locus_tag="ABE17_RS17640"
                     /old_locus_tag="ABE17_17310"
     CDS             complement(3684..3827)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="ABE17_RS17640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_17310"
                     /product="hypothetical protein"
                     /protein_id="WP_002013225.1"
                     /transl_table=11
                     /translation="MKNEVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFEEI
                     EY"
     gene            complement(3859..4887)
                     /locus_tag="ABE17_RS17645"
                     /old_locus_tag="ABE17_17315"
     CDS             complement(3859..4887)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002085304.1"
                     /locus_tag="ABE17_RS17645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_17315"
                     /product="serine hydrolase"
                     /protein_id="WP_197219249.1"
                     /transl_table=11
                     /translation="MYKYEKLISWVESIKETNQSSAAALCIIKDNKIVLEHYSGTHSNT
                     PTSKKVTISSQFNVASARKSYLGLMVAYAVYEGKIKSLDDEAVNYFKEYDPSLLGKTTI
                     RHLVTHSHGLNETDDGTAFREFEPGQAWAYRDINVRMVTHLIYQLYNKSFPELLKERVF
                     TPADFKKTGWRIKQDDNLVKVVVNPDEDAISSVGAVDDGSEKNLFVSAREFAFWGNLHL
                     NKGFLNGKQIVPKEVIALATSLQSPEYTNKELPQNGLFWFVQNEPALFSELGERVPKGS
                     YQILGITGPTILVIPKYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSSSKNTMV
                     RV"
     gene            complement(5001..5276)
                     /locus_tag="ABE17_RS17650"
                     /old_locus_tag="ABE17_17320"
     CDS             complement(5001..5276)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001869.1"
                     /locus_tag="ABE17_RS17650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_17320"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001001869.1"
                     /sec_met_domain="TIGR03601 (E-value: 4.7e-40, bitscore:
                     130.3, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEFQQELQALNLNAYQPGNVVYWDQQQNQYPYYYIQDDARRCGG
                     CGGGGRCGGCGGRCGGCGGGRCGGCVGCVGCVGCFSCSNCWNWWFI"
     misc_feature    5290..5305
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 26.71"
                     /tool="antismash"
     gene            complement(5476..5922)
                     /locus_tag="ABE17_RS17655"
                     /old_locus_tag="ABE17_17325"
     CDS             complement(5476..5922)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="ABE17_RS17655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_17325"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_000836819.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAIMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVTYSIKQFLGVIENQWQAKETLHHEINHIFEIDSNELKSDFTPISKESH
                     LAFHWIDFNKENINSYTIMPTPSVKELLEKKLSDELLTCWISNF"
     gene            complement(6295..6864)
                     /locus_tag="ABE17_RS17660"
                     /old_locus_tag="ABE17_17330"
     CDS             complement(6295..6864)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002065694.1"
                     /locus_tag="ABE17_RS17660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_17330"
                     /product="DUF4256 domain-containing protein"
                     /protein_id="WP_197219250.1"
                     /transl_table=11
                     /translation="MKESNKKELSLEQREELLQTLKNRFENNMNRHKGLEWANVQERLD
                     ANTEKLWSLNEMERTGGEPDVVDFGKEKGEYIFYDCSAESPKGRRSVCYDLEALESRKK
                     HKPENNAIDMATAMGIELLTEEEYRTLHNLENFDMKTSSWVQTPSDIRELGGALFCDYR
                     FGHVFVYHNGAESYYAARGFRGSLRV"
     gene            complement(7048..7512)
                     /locus_tag="ABE17_RS17665"
                     /old_locus_tag="ABE17_17335"
     CDS             complement(7048..7512)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136577.1"
                     /locus_tag="ABE17_RS17665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_17335"
                     /product="N-acetyltransferase"
                     /protein_id="WP_002168287.1"
                     /transl_table=11
                     /translation="MNVQLKIVTRENWEDALKLRVKENQIHLVPAVAVSLAKVYIKPDG
                     ENVEYIPFAIYDGDLIVGFIMHAFVRESTDMYWINGFIIDEKEQGKGYGKAALQEIINL
                     IKNNFKECEEIRLTVHKDNISAKKLYECYDFKPLGNVYDGEEVYRLLLLK"
     gene            complement(7590..8513)
                     /locus_tag="ABE17_RS17670"
                     /old_locus_tag="ABE17_17340"
     CDS             complement(7590..8513)
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="ABE17_RS17670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_17340"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_002127616.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATGPLTNEITTYYPEIHGPEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGEDLTIVDVGRSTSLAIAFNLWNELMLNIKGIYFMGGVFLEAGNVTPLA
                     EANVYGDPIASQIVFQQAKNITLFPLNVTNKTVLTPSVFTYILVNTQNPFKSIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVLAISGLVNPTFLNYTSRKVTVDICGETKGQTFADFRPH
                     SKSEGARIALQLDEEKFIQDFMKIML"
     misc_feature    8763..8779
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.88"
                     /tool="antismash"
     gene            complement(8797..9708)
                     /locus_tag="ABE17_RS17675"
                     /old_locus_tag="ABE17_17345"
     CDS             complement(8797..9708)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136580.1"
                     /locus_tag="ABE17_RS17675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_17345"
                     /product="DMT family transporter"
                     /protein_id="WP_002185732.1"
                     /transl_table=11
                     /translation="MHNKKWDFRIVCAHAFTILIWGTAFPGIRMGLEAYTPEHLTLLRL
                     LIASLILFLFSLIYKLRLPDLKDIPVIFIFGALGFTIYHIALNYGEKSVNAGPASLIVS
                     VTPILTAILASVFLNERMKLNGWIGGVISFVGIAFLSLSQGDSIQLNSGGLLILLAAIS
                     ESLFFVFQRPYLKKYGFLPFTIYTIWSGTVFMLIFLPGIYQEIVTAPIEITLSVIYLGV
                     FPTVLPYIALAYITSHASASEATSSLYLTPVTACIIAWIWLGEVPTFVSIIGGGITILG
                     VVIAHLTFNKDDYKQIESEKVW"
ORIGIN
        1 tgttgtgtct cggatatttt accaatttga gaagcgttgg aagaaatttg tattgtgcat
       61 tcgtcgatga tttctaagtt aacgttgttg tttgcccatt cactagccaa taaacttgac
      121 cgatctacac cttgtaaaat cttttttcta tctatattaa taatcgtttg ggattcattt
      181 ggaattaggc cggatatatt aggatatttc ccttcaataa gccttgaata caacgtaatt
      241 gtaccgaatt taaaaataat gtgactctct gaaagatata tgtatactaa atttgaatta
      301 ctgctcatta attttagtag ttcactaata gtggaacttg gtacaatgca atttaatttc
      361 gcatgtgaag aaatgggtat ttcacgtaaa gccagtctgt gcgaatcagt agcagcacaa
      421 attaatttgt tatgatcaat aacgaaatgt acgccagtaa gaactggtct agattcattt
      481 ttagcagttg caaagacagt ttgtttgaat acctcaatca gctgttccgt ttctacttgt
      541 atttctgtat gtgagtctaa aaatggtaca ttaggaaatt catttgccga gagtccattt
      601 aagtttaaag taatttcatc tgattgaatt gtaatcaact gctcattcat actttttatt
      661 aaaatctcac ttggcatttt cttaataatt tcaatgaaat actttgctgg tacaacaata
      721 cttcctgctt ttaaaatagt tgtaatttgt tcgtcttcaa ttgaaatagg tataaatttt
      781 tcaatgaaaa tattcgaatt acttgcgatt aaagtaattc cagattgatc tgctgttatc
      841 tttattccgg ataatatagg aattaaagtt tttgttgaga tagctttact tacttctgaa
      901 agagcttgtg taaattgttt gtgattaacg ataaactcca tttttccccc gccttaaatg
      961 aaatactatc tttatgaaga ttgatgaata atagttccat taaaaatata aggaactgtt
     1021 tctttctttt gtaaaaggct ttttaagtgt tctggtatac gtaaataagg ttctgctgaa
     1081 tctaattcca tccaggtaat ctcctcaatc tcttttggcc gtgatataca gatttcccct
     1141 ccggttattt ctcctaaaaa attaaagaag attgcatgat gtcctctctc ctcaaaaaag
     1201 gcttcactaa tggaacaaac cccttttacg gatatgtcta gccctgtttc ttctttcact
     1261 tcacgaattg ctgcctcttc taacgtttcc ccgaatttaa ctgctccacc tggtaaagtg
     1321 taataagaac cattttcccc tttgttttta accattaata gcttttcatg agtttcatca
     1381 tatagaagtg cgtatgtaac atttactttt ttcatatgta acccccttga atggtgaaaa
     1441 ttttctgtct ttattttatt ctatcattaa attgttttga ttcataaaaa gtaatttgag
     1501 taaataacta atacgaaacg atatcgtgtt catagacaca caaaagaaaa gagcatattt
     1561 tgaaaaagat taatcataat acactcttta atgattcatt tataaccttt tttctaaata
     1621 gtaatgatta tgccctttag gataatcttc acttacccct atcactttat atccatgctt
     1681 cttataaaaa ttaggggctt ggaaactaaa agtatctaat ttgattagcc tacattcttt
     1741 ttttattgca aattcttcaa taagattcat taaattgctt ccataacctt catgcctgta
     1801 ttcttcagaa acccatagga agtcaacata tacatggtgc caaaatgtga ttgccgttat
     1861 ccctcctact atttcttctt tttcattctt cactataaaa ctaatctttt ctgaaggtgt
     1921 tttttcttca tttgaaagtt gtttttggtt atattcaatt actttttctc ttatgtaatc
     1981 actatcttct tgtacccatt gctttactat ttgcatttta actatcacgc ccctttacat
     2041 tatttattca atttaataat cggaatttcc tatcttcttt aatgttagat tatgaaataa
     2101 gtacagggaa aaaattagat tttttgaatg tcattcattg tttatacatt tttttgtaaa
     2161 gcaaaagagc cctagtataa ggactctttc ttagcattaa gctatttttc gatttaacga
     2221 tttgatttcc ttcttctttt ctgcatttgg agggcggatt actatagaga taacgaaaga
     2281 tataacacat agaacaccga ttgtaatgaa ggttggctga aatccgccaa gtagtgctcc
     2341 aatgaatgat cctgcaagtg caccaaatcc gaaaccttgg taaacaatcc cgtagtttgt
     2401 actatgattt tttaatccga agaagtctcc cacaattgct gggaagatag tgatgttacc
     2461 accaaagcaa aaggcaacac ttgctacaca agcaaaataa attccgtaat ttagtggaat
     2521 aaaacttaaa gtgaagacgg ataatccaat aataataaat gtagccgaga cgatttttaa
     2581 tcgacctatt ttatctgata acgttccaag aataattcta cctattgtat taaagattgc
     2641 aatcattgcg acggcattag cggccgtcgc tgcactaaga ccaacgagct gcaccccgat
     2701 gtcttttacc ataccgatta aatacagtcc gcccatacat gatgtgaata acatgaaaaa
     2761 taagagatat acttgttttg ttttcatcat ttcacgcggc gtataatcat tgtgtaatgt
     2821 ttcagttgca gtgttactta caattgcttc acgtaagaag aatgatccta tcaatactaa
     2881 tagtaagacg ataatacccc aatataaaaa tgcttgtgat actccaagac tgttgataag
     2941 atttccgttt atatatctaa agattaagct tcccattcca tatgccgaaa cagatatacc
     3001 agaaataagt cctttacggt ttggaaacca tttaattaaa ttagatagtg atgtaatata
     3061 tgctgttcca tcagcgtatc caacgacaac accggctaat aaataaagta atggtaatga
     3121 ggaaacttgt gaactaagta ttaaaccgag tcctaaaaca attcctgcag tagcgataag
     3181 tttacgaagt ccaaattttt gttgtagctt tcctgcaaat agagttgaga atgataaaga
     3241 aaaactcgta attgagaacg tgatcgcgac tgaattgagg ttccatccaa acttacttac
     3301 aaggggttga ttaaataaac tccatgtgta aattgttcct aggccaattt gaacaatgat
     3361 tgtacctaga acaattagca atggatttat agatgtttgt ttcatgctaa ctgtcccctt
     3421 cttttatcat gacatgaatt ttcacagtta gtatatccat gataaattgc aagatactgt
     3481 acggaaaatg gaaattgagt tagcttacac aaaaagacac ttcatcaaga agtgcctttt
     3541 tgtaggttat atacagcatt taaagaaagc tttgttaaga taagacattt tcgaatgctt
     3601 atgtatatat gttatcaaat aatttgctga aagtgatgag acttaagacg tatttttctt
     3661 tcataaataa gtcggagaga aaattaatat tcaatttcct caaagtcttt cggctgcggt
     3721 acagtttgca tattagctgc atctttcgga tcgctataaa tcgcgttttg ttctgtgttt
     3781 acatccttta tattttgctt tttattttta ttcacttcat ttttcattaa agttcactcc
     3841 tttttattat aggagtagtt ataccctaac catagtattt ttactgctac taaatgtatc
     3901 agcgactaaa ttgctaaatt cacgtaaata atataaataa ttatccccac catagttgta
     3961 tcttttattg tacattttag caacgacgac attatatttt ggaatgacta atatcgttgg
     4021 tcctgtaatt cctaatattt gataggaacc tttaggaact cgctcaccaa gttcgctaaa
     4081 taaagcaggt tcattttgaa cgaaccaaaa caacccgttt tgaggtagtt ctttatttgt
     4141 atactctgga ctttgtaagc tcgtagcaag tgctataact tcttttggca caatttgttt
     4201 accatttaaa aaccctttat ttaaatgaag gttaccccaa aatgcaaatt ctctcgcaga
     4261 gacaaaaaga tttttctcgg agccgtcatc tactgcacca acactactta ttgcatcttc
     4321 atctgggttt acaacaacct taactaaatt atcatcttgt tttattctcc atccagtttt
     4381 tttgaaatca gcaggtgtga atacacgctc ctttaatagt tcaggaaaac tcttattata
     4441 cagttgataa ataagatgcg tcaccattct tacattaata tctctgtacg cccaagcttg
     4501 tcccggctca aattcacgaa aagctgtccc atcatcggtt tcatttaaac cgtgtgaatg
     4561 agttactaaa tgtcttatcg ttgtctttcc aagtaatgaa ggatcatatt ctttaaaata
     4621 atttacagcc tcgtcatcaa gagattttat tttcccctca taaactgcat aagctaccat
     4681 taatcctaaa tagctttttc ttgcggaagc gacattgaat tgtgaagata tagttacttt
     4741 tttactagta ggagtatttg aatgcgttcc actataatgc tctagtacaa tcttattatc
     4801 tttaataata caaagtgctg ccgcagaact ttgattggtc tctttaatgc tttctaccca
     4861 tgaaattaat ttttcatatt tgtacaaata aatatccccc taaaacatat aaaaataaca
     4921 gcatgaaaga aaagtttcaa tacattataa ctttgcatct gcacttttct ttcatgtgaa
     4981 atattataga tctataatac ttaaataaac caccagttcc agcaattaga acagctaaaa
     5041 caaccaacac agcctacaca acctacgcaa ccgccacaac gtccgccgcc acaaccacca
     5101 cagcgaccac cacaaccgcc acaacgtccg ccgccaccgc atccgccaca acgacgtgca
     5161 tcgtcttgaa tgtagtaata tggatattga ttttgttgct gatcccaata cacaacatta
     5221 ccaggttgat aagcattaag gtttaacgct tgtagttctt gttgaaactc attcattatg
     5281 aaaccctcca tttacaaatg taaattccgt ccaaattttc gagtttattt acttttaaaa
     5341 ataaagtaga aataaggcta tagactaaat gcgtacatca acaaaatatg actttacact
     5401 aggtgatgta ccttgtttat gagcctattt tctttatatt cttttagtgg acggaattaa
     5461 tataaataga gacaattaaa aattactaat ccagcaagtt aatagttcat cacttaattt
     5521 tttttctagt aattctttta ctgaaggtgt cggcattatt gtataggaat ttatattttc
     5581 tttattaaaa tcaatccagt gaaatgctaa atgggactct ttagatattg gagtgaaatc
     5641 ggattttaac tcatttgaat ctatttcaaa aatgtggtta atttcatggt ggagggtttc
     5701 tttggcttgc cattgatttt ctatgacgcc taaaaattgt tttatactgt aagttactcc
     5761 aagttcttct cgtaactctc ttattagtgc attctctgct gattccccaa tttcaacatg
     5821 accaccgggt aaaaaatagt gatggcctat gtattcagca actagtaatt tttcatcttt
     5881 tatcatgata gctcgtacaa tatgatggaa tttacttttc attttgtatt cctcctactc
     5941 attcacttta tacttgctcg aaaattttca tttaaaagcg ggataatata aatcataggt
     6001 gtttcatatg ggtgaatttc attgataatt gcaagagtac cttttacaag ttctcgttta
     6061 tacgttaatt aaaactaata attttgataa atgattctaa aaaagagtat gtttatgaag
     6121 aaaattatca aaacatactc ttaatatttt tagttgattc attcttttac aataaggttt
     6181 gattagccct attctttgca gcctcaaaat cgaagccgta gctaaagtat gcaatcacta
     6241 ctattcccta aggaaatcta ttctctactt caaatttacc tgcctgtaca aattttagac
     6301 tcttaacgaa ccacgaaaac ctcttgcggc atagtaggat tctgctccat tgtgatacac
     6361 aaagacatgt ccaaagcgat aatcgcaaaa aagggcaccg ccaagttctc taatatcaga
     6421 aggtgtttgc acccaactcg acgtcttcat atcgaaattt tcaagattat gtaatgtacg
     6481 atattcttct tccgttaata gttcaatacc catggcagtt gccatatcaa tagcgttatt
     6541 ttctggttta tgtttctttc ttgattctag cgcttcaaga tcgtaacaaa cacttctacg
     6601 acctttagga ctttctgctg aacaatcata aaaaatatat tcgccttttt ctttaccaaa
     6661 atcaacaaca tcaggttcgc caccagttcg ttccatttcg ttgagcgacc acaacttttc
     6721 agtattagca tccagccttt cttgaacatt agcccattct aaacctttat gacggttcat
     6781 attattctca aaacgatttt tcaatgtttg tagtaattct tcacgttgct ctagtgacaa
     6841 ctctttttta ttgctttctt tcatattagt tcctccattg atgtttttat taatgatagt
     6901 ttaagtatat ataaatactg ttttataata aaatgtttaa ataattgcgt gaattgagtt
     6961 tgcgataatt tatatgaatt agtacaacca attctccgca atatgaccct ttaacggaac
     7021 aacttattgt ttcaaaacag tacatcttta tttcaacaac aataagcgat atacttcctc
     7081 accgtcatat acatttccta atggtttaaa atcataacat tcatatagtt tctttgcaga
     7141 aatattatct ttatggacag ttaatctaat ttcctcacac tctttaaagt tattttttat
     7201 taagttaatg atttcctgta atgctgcttt tccatatcct tttccttgct ctttttcatc
     7261 aatgataaat ccgttaatcc aatacatatc tgttgactct cgtacaaaag catgcataat
     7321 aaaaccaact ataaggtcac catcatatat agcgaatgga atatattcga cattttcacc
     7381 atctggtttt atgtatactt tagcaagtga tactgccact gcgggaacaa gatggatttg
     7441 attttcttta actcgtaact ttaatgcgtc ttcccaattt tctctcgtaa caatttttaa
     7501 ttgaacgttc actattttaa gctcctttaa aaagagaatt tatcttttat attcaacgga
     7561 gtgaaatgat tccctttttc gacatcaact tataacatga ttttcataaa atcctgaata
     7621 aatttttcct catctaattg taacgctata cgcgcccctt cagatttaga gtgtgggcga
     7681 aaatcagcaa aagtttgtcc cttcgtttcg ccacatatat ccacagtcac ttttcgggat
     7741 gtataattta aaaatgtagg attcactaaa ccgcttatag caagtacatc gtgtaataat
     7801 ggcccttcaa tagatggatt gagtttttgg tatgctgata aataataatc gtacatgggt
     7861 ttcataatcg acttaaatgg attttgtgta ttaactaaaa tgtaagtaaa tacacttggg
     7921 gttaaaaccg ttttatttgt tacatttaaa gggaataacg ttatattttt cgcttgctga
     7981 aatacaattt gacttgctat aggatctccg tacacgtttg cctctgctaa tggagtaaca
     8041 ttccctgctt ctaaaaagac accacccata aaataaattc cttttatatt taacatcaat
     8101 tcgttccata aattaaaagc aatcgctaac gaggtagatc ttcctacatc gacaattgtt
     8161 aaatcttctc catactttac aagaatcgca gcaatactac caaaattgta tataggaata
     8221 gataacacac tttccggtac atggatgggc cctaatcctt ctggtccatg gatttcaggg
     8281 taatacgttg taatttcatt tgtaagtggg cctgttgcac cgctaattac aggaatatct
     8341 tgtctgtttg ctaactgtaa aatatatgcg gcattatgag cggcatgtat atgttcaaca
     8401 tttccatatc cagttacgat accaacaatt tcaatttcag gatgcagtaa gccatacata
     8461 attgcaaacg aatcatcaat acctgggtct ccaaaaaata aaaccttctc caagaaatgc
     8521 actcctttca taggtaagat aattatgtat atgtggaaaa aaattaaatc tatacagttc
     8581 taagcaaata ttaatggtga gtaatgtatt gataaaaaag aactagcatt atgctagttc
     8641 ttttgcagat aaataatcta ccagtttaat taaatctcgt acaaatattg gactaactga
     8701 ttttaatata gatttttttc actatattta tagaattgaa aacctgttat taatccgaaa
     8761 ttttgcattc aaattgcaaa tactcgtagg gatgtcttac catacctttt cactctcaat
     8821 ttgcttataa tcatctttat taaatgttaa atgagcaatc acaactccaa gtatagtaat
     8881 tccccctcca attatcgaaa caaaggttgg cacctcacct aaccatatcc aagcgataat
     8941 acatgcagtt actggtgtta aatatagaga acttgttgcc tcagaagcac tggcatggga
     9001 tgtaatatat gctaatgcaa tatacggcag tactgtcggg aacacaccta aatatataac
     9061 actcaaagta atttctatag gagctgttac gatttcttga taaatccctg gcaaaaaaat
     9121 aagcataaat acagtaccag accaaattgt atatattgta aatggcaaga aaccgtactt
     9181 ttttaagtat ggcctttgaa aaacgaaaaa tagactttct gaaattgccg ccaataatat
     9241 tagtaatccc ccactattca attgaatgga atctccttga cttaacgata aaaaagcaat
     9301 tcctacaaaa ctaattacac caccgatcca accatttaat ttcattcttt cattcaaaaa
     9361 aacagaagca agaattgccg ttaatatagg tgttactgat acaattaaac tagcaggtcc
     9421 agcattcaca ctcttttcac cgtagttcag tgcaatatga taaattgtaa atccaagagc
     9481 accaaatata aaaattactg gaatatcttt caaatcaggt aaacgtaact tgtatataag
     9541 tgaaaacaga aaaagaatca aagaagcgat taataatcgt agcaaagtaa ggtgttcagg
     9601 ggtgtaagct tcaagcccca tccgaattcc tggaaaagct gttccccata taagaatcgt
     9661 aaaagcgtga gcacatacaa tacgaaaatc ccatttttta ttatgcatat attctctccc
     9721 tctccaataa accagccatt ttctaattac caattatttc ttttcatcat atttaatagt
     9781 tgatacaatg tcttcaacca gttaatcctc gaaaaaacca accactttgc atttttaaaa
     9841 tgaatgaggg agtttttaat atgtctactg ataaaaaact gcctaaatat cggcagataa
     9901 tagactatat gaaagaaaaa attgaatacg gagaatggcc gattggaagt aaggttccta
     9961 gccagcggca gttagcgaaa ttatttcatg taaatcgtag tactgtaata attgcactag
    10021 aagaacttat ggcagatggc ttaattcaag ggaaaatcgg gtcaggaaca attgtaacga
    10081 ataatacatg gtcattatta gccagcaatc ccccgcccga ttggagtgaa tatgtaaagg
    10141 ctggtattca taaaccaagc aagttaatgg tacgagaaat aaatgaagca gaagcaaata
    10201 aaaaattgat tcatcttagt aaaggagaac ttgcacctca tcttttcccg ttagaaacta
    10261 tgcaattcat tatgcg
//
