LOCUS       NZ_LDGT01000039        10300 bp    DNA     linear   CON 30-AUG-2024
DEFINITION  Bacillus mycoides strain G25-84 G25-84_contig000039, whole genome
            shotgun sequence.
ACCESSION   NZ_LDGT01000039
VERSION     NZ_LDGT01000039.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 352559)
  AUTHORS   Zheng,Z., Zheng,J., Zhang,Z., Peng,D. and Sun,M.
  TITLE     Nematicidal spore-forming Bacilli share similar virulence factors
            and mechanisms
  JOURNAL   Sci Rep 6, 31341 (2016)
   PUBMED   27539267
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352559)
  AUTHORS   Zheng,Z. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2015) Microbiology, Institute of Life Sciences and
            Technology, Shizishan str., Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 1.3.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 170.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015845055.1-RS_2024_08_30
            Annotation Date                   :: 08/30/2024 03:02:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,776
            CDSs (total)                      :: 5,620
            Genes (coding)                    :: 5,416
            CDSs (with protein)               :: 5,416
            Genes (RNA)                       :: 156
            rRNAs                             :: 13, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 1 (5S, 16S)
            partial rRNAs                     :: 13, 14 (16S, 23S)
            tRNAs                             :: 110
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 79 of 204
            Pseudo Genes (incomplete)         :: 129 of 204
            Pseudo Genes (internal stop)      :: 54 of 204
            Pseudo Genes (multiple problems)  :: 50 of 204
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:03:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 51772
            Orig. end                         :: 62072
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LDGT01000039.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10300
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10300
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10300
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5300](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5300
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(70..86)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.99"
                     /tool="antismash"
     gene            761..1366
                     /locus_tag="ABE17_RS16500"
                     /old_locus_tag="ABE17_16175"
     CDS             761..1366
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853587.1"
                     /locus_tag="ABE17_RS16500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_16175"
                     /product="PRK06770 family protein"
                     /protein_id="WP_002127350.1"
                     /transl_table=11
                     /translation="MKTLFKIIGIIAGMAVIGVGVTYGMLYYLNNSKPAAKKASPAAPA
                     VEVLADSNVKAEDAKILENGNYSLPNSGFNKNFKWTDENIQTALHEMAHQKAKADQKWG
                     YIFITQERIESLLEIINSNDVAQKSTYVDILERWKQGKYEKVDVDHNKIWKLQSGNLGE
                     GKGVMSEKEQKELINQVFIQKGTYSGSKLIAGEVQANK"
     gene            complement(1472..2344)
                     /locus_tag="ABE17_RS16505"
                     /old_locus_tag="ABE17_16180"
     CDS             complement(1472..2344)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016095293.1"
                     /locus_tag="ABE17_RS16505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_16180"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_002127351.1"
                     /transl_table=11
                     /translation="MEMRHVKTFCAIVKYGSFSKAAHALGYAQSTVTAHIKALENDLNI
                     PLFDRLGKKILLTKAGHQFHPYALELLTIYDKAQEIPQNSDHLEGTLAITSNESLAVYR
                     LPQLLRTYKQENPKVNIVLETNTNEQALKKLHEGETDVIFIIGESIEHNDFITCNLCNE
                     TFGWIFPAHFPSNTDPLLLLQDTQFIFTEQSCGYRPMVDRFLRQSGRNPNKTFETSNVE
                     VIKQSVMCELGISILPYIVVQESNQKEQLCFQPIETPAVIQSHVIYHKSRWISPVLQSF
                     LTLLERIEK"
     gene            2474..3517
                     /locus_tag="ABE17_RS16510"
                     /old_locus_tag="ABE17_16185"
     CDS             2474..3517
                     /EC_number="1.2.1.11"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1219: N-acetyl-gamma-glutamyl-phosphate reductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200507.1"
                     /locus_tag="ABE17_RS16510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_16185"
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="WP_197219203.1"
                     /transl_table=11
                     /translation="MSEKSYHVAVVGATGAVGQKIIALLERVTKFNIEEITLLSSKRSA
                     GKIVQFKGREIKIQEAKSTSFEGVDIAFFSAGGEVSRQYVNHAVSSGAIVIDNTSEYRM
                     AHDVPLVVPEVNAHTLREHKRIIAVPNCSALQMVTALQPIRKSFGLERIIVSTYQAVSG
                     SGIHAIHELKEQAKSMLTGEEVKSTILPVKKDKKHYPIAFNVLPQVDIFTDNDFTFEEV
                     KMIQETKKILEDPNLKMAATCVRVPVVSGHSESVYIELEKEATVAEIKEVLLNATGVIL
                     QDNPSEQIYPTPLYAEGKRDTFVGRIRKDPDTSKGFHLWIVSDNLLKGAAWNSVQIAET
                     LVENGII"
     gene            complement(3764..4057)
                     /locus_tag="ABE17_RS16515"
                     /old_locus_tag="ABE17_16190"
     CDS             complement(3764..4057)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002168401.1"
                     /locus_tag="ABE17_RS16515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_16190"
                     /product="hypothetical protein"
                     /protein_id="WP_002168401.1"
                     /transl_table=11
                     /translation="MCNSKPIDELTIEDLKQNPIWEWAIDEAENEECDETWVKPVETIN
                     FTEELNGSIVLGELIIHNDEKFPMMCSIDIENNEVLISSIVFITKKKMSILL"
     misc_feature    complement(4288..4305)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.83"
                     /tool="antismash"
     gene            5001..5300
                     /locus_tag="ABE17_RS16520"
                     /old_locus_tag="ABE17_16195"
     CDS             5001..5300
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002189588.1"
                     /locus_tag="ABE17_RS16520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_16195"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_197219204.1"
                     /sec_met_domain="TIGR03601 (E-value: 8.2e-39, bitscore:
                     126.4, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNFQQELQALSLNDYQPGNIVYWDQQNQYPYYYITDDARRCGGG
                     GRCGGRCGGCGGGRCGGFRCFGCFGCFSCFGCGGCSNCSNCFDGFNGTTVTFEY"
     gene            complement(5609..6511)
                     /locus_tag="ABE17_RS16525"
                     /old_locus_tag="ABE17_16200"
     CDS             complement(5609..6511)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003203974.1"
                     /locus_tag="ABE17_RS16525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_16200"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_197219261.1"
                     /transl_table=11
                     /translation="MISIELRQLEYFLAVSKELHFTKAAEKLNISQPSLSQQIRALEHE
                     VGMPLFDRIGKKISLTEAGRVLLSHSKTIFHEVEQARSAIQDLNGLHHGSLTIGSLLTV
                     VNYLLPPAILDFNKQYPNIELSVLGLRTGEIREKLLQNELDMGITFLPVQDKEIVSIPL
                     YKSELTLVVPTSHSLTERKYVSIEELQDYPLILLPKNFYLTELITSHCQNFNFKPKPIL
                     EISTMESLIQMVAQGMGITVLPKPYIDFLQNEHIQAIKIENPTPTINIGLIYRRDKYMC
                     AATRKFIEQLKITVHSLQK"
     gene            6641..7966
                     /gene="gabT"
                     /locus_tag="ABE17_RS16530"
                     /old_locus_tag="ABE17_16205"
     CDS             6641..7966
                     /EC_number="2.6.1.19"
                     /GO_function="GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (34-374). E-value: 4.7e-122.
                     Score: 399.2. Matches aSDomain:
                     nrpspksdomains_ABE17_RS16530_Aminotran_3.1"
                     /codon_start=1
                     /gene="gabT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200502.1"
                     /locus_tag="ABE17_RS16530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_16205"
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_002031963.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6.3e-133, bitscore:
                     437.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKSSVLPGSYSQVLQKRYEEAVPTGIYHLTPLYVKEARGAIITD
                     VDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAESFIHPCFHVTPYENYITLAEKINEK
                     VPGTSKKKTMFANSGAEAIENAVKIARKVTGRSKVISFERGFHGRTLMTMSLSTKETPY
                     KAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQVIYQFEKFLEEVSPEKVAAIVMEPV
                     QGEGGFIVPSISFVKRLREICNQYGILMIMDEVQTGFGRTGKWFATEHFHIEPDIMTLS
                     KSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGSPLSCAAGLAVFEIMEEENLVERAQQ
                     IGNRIMSHLHQMQLKYEMIGDVRGIGAMVAMELVTNRITKEPAKELTVKLIEDFWKNGL
                     ISIGAGIFDNVLRFLPPLVISNEEIDRGFEIINESFEKLCRK"
     aSDomain        6743..7762
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ABE17_RS16530_Aminotran_3.1"
                     /evalue="4.70E-122"
                     /label="ABE17_RS16530_Aminotran_3.1"
                     /locus_tag="ABE17_RS16530"
                     /protein_end="374"
                     /protein_start="34"
                     /score="399.2"
                     /tool="antismash"
                     /translation="VKEARGAIITDVDGNQFIDFAGGIGMQNVGHCHPKVVRAVQKQAE
                     SFIHPCFHVTPYENYITLAEKINEKVPGTSKKKTMFANSGAEAIENAVKIARKVTGRSK
                     VISFERGFHGRTLMTMSLSTKETPYKAGFGPFVSDMHILPYPYYYRAEEGVSPEEVDSQ
                     VIYQFEKFLEEVSPEKVAAIVMEPVQGEGGFIVPSISFVKRLREICNQYGILMIMDEVQ
                     TGFGRTGKWFATEHFHIEPDIMTLSKSIAGGLPLSAIVGRAELMDIPGRGQIGGTFAGS
                     PLSCAAGLAVFEIMEEENLVERAQQIGNRIMSHLHQMQLKYEMIGDVRGIGAMVAMELV
                     "
     gene            7981..8670
                     /locus_tag="ABE17_RS16535"
                     /old_locus_tag="ABE17_16210"
     CDS             7981..8670
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002031964.1"
                     /locus_tag="ABE17_RS16535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_16210"
                     /product="MOSC domain-containing protein"
                     /protein_id="WP_002168398.1"
                     /transl_table=11
                     /translation="MTKVYEILSLNLGLPKEVMYGGKLIQTGINKKQVKEPVFLSFLKF
                     NGDGQADLVHHGGVDKAVCVYTGDHYPYWEKELNQELVYGAFGENITVSGMREEDVCIG
                     DTFQLGEAIVQVTQPRQPCFKLAKKYNIPKLPLYFQDTGYTGFYFRVLKEGWILPVDTL
                     KLLKLDPKGVSILFANRIMHKEKQNSEGLKRILEVNALSTSWRKTFEKRVKGEEISTKE
                     RLEGTKE"
     gene            8933..9817
                     /locus_tag="ABE17_RS16540"
                     /old_locus_tag="ABE17_16215"
     CDS             8933..9817
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526760.1"
                     /locus_tag="ABE17_RS16540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABE17_16215"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_088294179.1"
                     /transl_table=11
                     /translation="MGNVEKMANFLLEEPRREQLFPLFEEVFGITSQTLQDFSEKGYWD
                     YTYKALSFLQDDNAIANVAVFSLPLLINGEKINATGIQSVMTHPNYRRRGLMKQLFSKM
                     IEEIDEQCECAVLFTENPELYKPFGFQIVQEYLMTVSYENKGYKDSQLKKLDFYNEEDR
                     QLIHEIIENGQRLSNKFSTLNYRSSFYLNMYDSTWNENLYYSEKLDALIVFTVENQKLK
                     LFGVFAPVIPILDELCEEISGEFTEIEFYFYPDQLGIDDVQYTELQTSKYLMVRSNKEL
                     DFKGYKFPVLTEF"
     misc_feature    complement(9901..9917)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.8"
                     /tool="antismash"
ORIGIN
        1 tggtattttg aaaaacaagt agagcgctgg aaaatagcaa tacagaagtc tcagcaatat
       61 gaattagtaa tactcgatgg ggatatatat cagccactta gttataattg gtgttttcgt
      121 tttgatatat ttaatcagcc gttatcttta atagaaaact tttataaaga aaagatgata
      181 aatagagaaa ttggttttcc agatcaatat ttttatttat atacaagtga tgaagagctt
      241 agaaaacgga aagagtttga tgggacgaaa aaaagaagaa actttgaaaa acatttacat
      301 atatctaaag catttcagcg ttattatgaa aatctcaata ccgtcacaga cgggtactgt
      361 aaattgattg aagcaaaaag tataaagtcg aatgaattag aaattgtgaa aagtttaaac
      421 agcttaaaag tatgtgaaga aagacgtttt gatgtttcga ggttagatgc tattacggag
      481 tggttaaaag aaaatcgtgc ataaagtaaa aacaacgagt gttttagaag gcgctcgttg
      541 tttttttgtt tccatataaa tataaaggaa aattttgtag aatatcttta ttttttaaca
      601 gaataatctt tcaatttcac gaaaaaagaa atacaatata aggaaatata tataaataat
      661 atgatagggg atgggatgaa ctattttgaa tgaaggaact gttttatata agaaataatg
      721 aggtagtaag aacagagata tttgggagga aacaagcaag atgaaaacat tatttaaaat
      781 cataggaatt atagctggta tggcggtaat cggggtaggt gtaacgtatg gtatgctgta
      841 ttatctgaat aatagtaaac cagctgcgaa aaaggcatca ccagccgctc cggcagtaga
      901 agtgctagct gatagtaatg taaaagccga ggatgcaaag atattagaaa atggaaatta
      961 ttcattgccg aatagtggtt ttaataaaaa cttcaaatgg acagatgaga acatacagac
     1021 agcattacat gaaatggcac atcaaaaagc gaaagctgat caaaaatggg gctatatttt
     1081 tattacacaa gaacgtattg aaagtttact tgaaattata aatagtaatg atgttgcaca
     1141 aaaaagtaca tatgtggata ttttagaacg atggaaacaa ggaaaatatg aaaaggttga
     1201 tgtggaccac aacaagattt ggaaattaca aagtgggaat ttaggagaag ggaaaggagt
     1261 aatgtcagaa aaggagcaaa aagaattaat taatcaagtt tttatacaaa aaggtacata
     1321 ttcaggtagt aaattaattg caggtgaggt acaagctaat aaataatata catattgatt
     1381 ttgaaacacc tgtattgtta tgaacacaaa tgttttaaca aaaatgagta attgcttttt
     1441 agagcagtta ctcatttttg taattactcg cttacttttc tattctctct aataatgtaa
     1501 gaaacgattg caaaactggg gatatccatc tagatttatg atagattacg tggctttgaa
     1561 tgacggctgg agtttcgatg ggctgaaaac agagttgttc tttttgatta ctttcttgta
     1621 caacgatgta agggagaata gaaattccta actcacacat aacggattgt ttaatgactt
     1681 caacattgga agtctcaaat gttttattag gatttcttcc gctttgacgt aaaaaacgat
     1741 ctaccattgg tctatagcca cagctttgtt ctgtaaatat aaattgagta tcttgtaata
     1801 aaaggagtgg atcagtgttt gaagggaaat gagcagggaa aatccatcca aatgtttcat
     1861 tacataaatt gcacgtaata aaatcattat gctctatact ttctccaatt atgaagataa
     1921 catcggtttc tccttcatgt aattttttca atgcttgctc attggtattt gtctcaagta
     1981 cgatatttac tttcggattt tcctgtttgt aagtacgtaa taattgtggt aaccgataca
     2041 ctgccaatga ttcattagat gtaatagcca atgttccttc taaatggtca ctgttttgag
     2101 ggatttcttg cgctttatca taaatagtaa gtaattctaa tgcatacgga tgaaattgat
     2161 gacctgcttt tgtaagaagt attttttttc ctaatcgatc gaatagggga atgtttaaat
     2221 cgttctctaa tgctttaata tgggctgtca cagtagattg cgcataacct aatgcatgag
     2281 cagctttaga aaagctacca tatttaacaa tggcacaaaa tgttttaaca tgtctcattt
     2341 ccataaatga tacctctttt attatcatga tttgtgaatg attatatcat gtatttcaat
     2401 ttttcaaatc attaaatttc ttttaaaatt aagttgtgga gaaattcatt caattgaaat
     2461 tggaggagaa aaaatgagtg aaaagagcta tcatgtagct gtagttggag ctacaggcgc
     2521 agtagggcaa aaaatcattg cattgttaga gagagtaacg aaatttaata tagaagaaat
     2581 tacattactc tcgtcaaaac gatctgctgg taagatagta caatttaaag gacgagagat
     2641 taaaatacaa gaggcaaaat caactagttt tgaaggtgta gacattgcct tttttagcgc
     2701 tgggggagaa gtttctagac aatatgtaaa tcatgcagtt tctagtggtg caatcgtaat
     2761 tgacaacaca agtgaatacc gaatggcaca cgatgtacca ctcgtcgttc cagaagtaaa
     2821 tgcacatact ttaagggaac ataaaaggat cattgcagtt ccgaattgct cagcactaca
     2881 gatggtaaca gctcttcagc caattcgaaa atcatttggt ttagaacgaa ttattgtttc
     2941 aacgtatcaa gccgtatcag gctcaggaat tcatgcgatt catgaattaa aagaacaagc
     3001 taagtcaatg cttacaggtg aagaagtgaa gagcactata ttaccagtga aaaaagataa
     3061 aaaacattat ccaattgcat ttaatgtatt gccgcaagtg gatatattta cagacaatga
     3121 tttcacattc gaagaagtaa agatgattca agagacgaag aaaattttag aagatccaaa
     3181 tctgaaaatg gcagctactt gcgtcagagt tccagttgta tcggggcatt ctgaatcagt
     3241 ttatattgaa cttgaaaaag aagcgaccgt tgcagaaatt aaagaagtac tactgaatgc
     3301 aacaggtgtt attttacaag ataatcctag tgaacaaatt tatccaacgc cattatatgc
     3361 agagggtaaa agagatacat ttgtaggtag aatacgtaaa gatcctgata catcaaaagg
     3421 attccatctt tggatcgttt ctgataattt attaaaaggt gctgcttgga actcagttca
     3481 aatcgcagag acattggtgg aaaatgggat tatttagtat ggatttatat aaatattgaa
     3541 aatagaagga tatttgggaa accgttaatt ataatggttt cctgtttttt acttaatgag
     3601 tattttcctc ttacttcaat taaaatttaa tttaagttag tttttatagt gtttttataa
     3661 atatcaactt tatctgcaga gaattttaaa aatctaggct ctccgttaat tgtgagatta
     3721 atattaagtg ataaaggcat ttcaaccttt ttcactacat cttctatagc aatatactca
     3781 tcttcttttt cgttataaaa acaatggaac taattaatac ctcattattt tcaatatcaa
     3841 tactacacat catagggaat ttctcatcat tatgtattat taattcgcca agtactattg
     3901 aaccgtttaa ttcttctgta aaatttattg tttccactgg ttttacccac gtttcatcgc
     3961 actcttcatt ttccgcttca tctattgccc attcccatat tgggttttgt ttcaggtcct
     4021 caatagttaa ttcatcaatt ggtttgctat tacacattaa tatatactcc ttttgccatg
     4081 aaaatggatg agtaaaaaat ttattcatta attgtaaaat gtggctattt gcaatcctat
     4141 aaatacaagt ttgctcgttt caataaatag ttgatcgcaa ataagaagtt tcgatgtaga
     4201 gtattatcca tttgatatta ttaaatctag ttttgtaatg ttgaacttat ttttacaacc
     4261 tattaaaagt ctattcttac ggtaatctta acaaggtttt aactagacag taaataatgc
     4321 cttttgaaaa ggaaaaccgt ggttgtgatg ggggaaatag caaaaaacat cgctgatttg
     4381 ccattcataa aaatagactt ttcttagcag aaatcactaa ttataaatac ttataaaatt
     4441 agctctgata tagcagatat tgaagcgaag aaaaataaag atgggagtgc atattggaaa
     4501 tacgaagatg accaaacaaa attatggaat atacgactat agaactagaa aatgatgaca
     4561 taaatctaaa tattattgtt taaattcata ttttataatg tccatttagc ccgcattaac
     4621 tgcccgtaaa ccccctctga ttaaagtttc actttataaa aatgagccca tagtaaaaat
     4681 aacctaaaaa taggcatatg aacaaggtgc atcatctagt gataggtcat attttgttgg
     4741 tgtacgtatt gaacttagtt tatcccgcta tttgtgggca gtaaaactcc cacctcaaaa
     4801 ttcaactgga gcaaagaagt taggtgggag ccctgctgcc cgtaaacgcc cgattggtgc
     4861 gggctaataa tcagtggggg atgaacaaaa cccctactga ttaaagtttc actttatagc
     4921 gctatttcta cttagattta acgaacagaa atataggaaa gtatcgatgg ggttttagtt
     4981 tataaacgga ggtcatgaag atgaataatt ttcaacaaga actacaagcg ttaagcctta
     5041 atgattatca gcctggaaat attgtgtatt gggaccagca aaaccaatat ccatattact
     5101 atattacaga tgatgctcgt cgctgtggcg gcggtggtcg ttgtggtggt cgttgtggtg
     5161 gttgtggcgg tggtcgttgt ggtggattcc gttgttttgg ttgcttcggt tgctttagtt
     5221 gttttggttg tggtggttgc tctaattgct ctaattgttt tgatggtttt aatggtacta
     5281 ctgtaacatt tgaatattga taagagacaa aaaggaatcc attttgaaaa aagattgatc
     5341 gaaacggatt cctactttgt gaagcattat ataattttta taaaaatgtg ctaacaagtt
     5401 tgcaaacgga cttgctaaca cctatacgaa atagacataa aatatcgctg atgtgtagct
     5461 taataatacg ataataattt agcacttata atgaataata ggttattaga aaagaaacca
     5521 taaaatgtaa tgttttatat cattataaaa agagattatt ctaataggtc gttgtgtaac
     5581 ggctgcatag gataatctct ttttatgctt atttttgtaa agaatgtact gttattttga
     5641 gttgctcaat aaatttccgg gtagcagcgc acatatattt atctcttcta taaataagcc
     5701 ctatatttat tgttggagtg gggttttcaa ttttaatagc ttgtatgtgc tcattttgca
     5761 aaaagtctat atacggtttt ggtaacacgg taatccccat accttgtgcg accatttgaa
     5821 ttaaagattc cattgtacta atttctaaaa ttggctttgg tttaaagtta aagttttggc
     5881 aatgagatgt aattagttct gttaaataga aatttttagg tagaagaatt aaaggatagt
     5941 cctgtaattc ttcaatagat acatacttac gttcagttaa agaatggctg gttggcacaa
     6001 ctaatgtaag ttcactttta tatagtggga tagaaacaat ctctttatct tgcaccggga
     6061 gaaatgtaat acccatatct aattcatttt gtaataattt ttcacgaatt tctcctgtac
     6121 gaagccccaa cacagagagt tcgatatttg gatattgctt attgaaatct aatatagctg
     6181 gtggtagtaa ataatttact acggttaaca aagatcctat tgttaaggaa ccatgatgta
     6241 atccatttaa atcttgaatg gcagaacgag cttgttcgac ttcgtgaaag attgttttac
     6301 tgtgcgataa taaaactctt cctgcttcag ttaaagatat ttttttacca attcgatcga
     6361 aaagtggcat gcctacttca tgttctaatg cacgtatttg ttggcttagt gaaggttgtg
     6421 aaatatttaa tttctcggct gcttttgtga aatgtaattc tttcgaaaca gctaaaaagt
     6481 attctaattg tcgtaattcg attgaaatca cctcaagttt aatcatagga tatacctatc
     6541 attattatat aaataattga attgaacaat ggttttgtgg gatatataat cactttatca
     6601 gaaatggatt gattaaaaaa ttagatgagg tgaagaagaa ttgttgaaat ctagtgtatt
     6661 accgggcagt tattcacaag tacttcaaaa aaggtatgag gaggccgtac cgacaggaat
     6721 atatcattta acgcctttat atgtaaaaga agcaagggga gcgataataa cagatgtaga
     6781 tggaaatcag tttattgatt ttgctggtgg aattggtatg caaaatgttg gacattgtca
     6841 tccgaaagtt gtaagagcgg tacaaaaaca agcagaatca tttatacatc cttgttttca
     6901 tgtaactcca tatgaaaatt atattacttt agcggagaaa attaatgaga aagtgccagg
     6961 tacaagtaag aaaaaaacga tgtttgccaa tagtggtgcg gaagcgattg aaaatgctgt
     7021 aaagatcgca cgtaaagtaa cagggaggag taaagtaatt tcgtttgaac gaggatttca
     7081 tgggagaaca ttaatgacaa tgtctttatc gaccaaagaa actccatata aagcaggatt
     7141 tggtccgttc gtttcagaca tgcatatatt gccgtatccg tactattaca gagctgaaga
     7201 aggagtttct cctgaagaag tggattctca agtcatctat cagtttgaaa agtttttaga
     7261 agaagtatca cctgaaaaag tagcagcgat cgtaatggaa ccagtacaag gagagggtgg
     7321 gtttattgtt ccttccattt catttgtaaa acgattacga gaaatttgta atcaatacgg
     7381 tattttaatg ataatggatg aagtgcaaac tggatttggc cgaacaggga aatggtttgc
     7441 aacggaacat tttcatatag aacctgacat aatgactttg tcaaaatcaa ttgctggcgg
     7501 tttaccatta agtgctattg tcggtcgtgc ggagttgatg gacattcctg ggagaggaca
     7561 aattggtgga acttttgccg ggagtccgtt atcatgtgca gccggtcttg ctgtatttga
     7621 aataatggaa gaagaaaatc tagttgaaag ggctcagcaa attggaaata gaataatgtc
     7681 acatctacat caaatgcaat taaagtacga aatgattggt gatgttcgtg gaatcggtgc
     7741 gatggttgca atggaacttg taacgaatcg cataacgaaa gaacctgcaa aagaactaac
     7801 agtaaagcta attgaagatt tttggaagaa tgggcttata agtattggag cggggatatt
     7861 tgataatgta ctacgctttt taccacctct agttatctca aacgaagaaa tagatagagg
     7921 atttgaaata ataaatgagt cttttgaaaa gttgtgccgc aagtaagagg agggatttga
     7981 atgactaaag tatatgaaat cctatcttta aatctaggat tgccaaaaga ggttatgtat
     8041 ggtgggaagt taattcaaac agggataaat aaaaagcaag taaaggaacc tgttttttta
     8101 tcatttttaa agtttaatgg agatggacag gcggatttag ttcatcatgg aggggtagat
     8161 aaagcggttt gtgtttatac tggtgaccac tatccttact gggaaaaaga gttgaatcag
     8221 gaacttgtat atggggcttt cggtgaaaat ataacagtaa gcggaatgcg tgaagaagat
     8281 gtttgtattg gtgacacgtt tcaactcgga gaagcgattg tacaagtaac gcagccaaga
     8341 caaccttgtt ttaaattagc caagaaatat aatattccaa agttgccatt atattttcaa
     8401 gatacaggat acacaggttt ttattttcga gtattaaaag aagggtggat attaccggta
     8461 gatactttaa aattgttgaa gcttgatcca aaaggtgtgt caattttgtt tgcgaatcgc
     8521 ataatgcata aagaaaaaca aaactctgaa ggattaaaaa ggattttaga agtaaatgca
     8581 ctgtctacta gttggagaaa gacgtttgaa aagcgagtga agggagaaga aattagtacg
     8641 aaggagcgac ttgagggaac caaagaatag gtgaattata tttgcataaa atggtgtatt
     8701 tctattatcg gaaagataag taggaagcac catttgttat ataataaaca tgatattatt
     8761 caaaaatctt aatactagtt tattcgactt gttttttaat aatcaaaaca taaaataaat
     8821 taaaccattt ttaaaagttt tatgatacta tattgatttg aaaggcctat tttttgaggg
     8881 taggtcatat aagtatatct aaattaatgg aatcgacgta tggagggaca acatgggcaa
     8941 tgttgaaaaa atggcaaact ttctattaga agaaccgaga agagaacagt tatttccttt
     9001 atttgaagaa gtgtttggga ttacgagcca aacattgcag gatttttcag agaaagggta
     9061 ttgggattat acatataaag cattatcatt tttacaagat gataatgcga ttgcaaatgt
     9121 tgcggtattt tcacttccgc ttttaattaa tggtgaaaaa ataaatgcaa cgggtattca
     9181 atcagtaatg acacatccga attatcgtag acgaggatta atgaagcaat tgtttagtaa
     9241 gatgatagag gaaattgatg agcaatgcga gtgtgcggtc ttgtttactg aaaatccgga
     9301 actatataaa ccatttggtt ttcaaattgt gcaagaatat ttgatgacag tatcatacga
     9361 aaataaaggc tacaaagatt ctcaacttaa aaagttagat ttttataatg aagaggatag
     9421 acaattaata catgagatta ttgaaaatgg ccaaaggctt tctaataaat tttcgacgtt
     9481 aaattaccgc tcttcgtttt atttaaatat gtatgattca acatggaatg aaaaccttta
     9541 ttattcagag aaattagatg cgctgattgt ttttacagta gaaaatcaaa aactaaaatt
     9601 atttggagta tttgcgccgg taattccgat tttagatgaa ttatgtgagg aaatctctgg
     9661 ggaatttacg gagattgaat tttatttcta cccagatcaa ttaggaattg atgatgtaca
     9721 atatacagaa ttacagacta gtaagtattt aatggttcga agtaataaag aattagattt
     9781 taaaggttat aaattcccgg tattaactga gttttaaata ttaatttaaa tgctttttta
     9841 tggaggaatt atgaaaacat catatatacc aaatcatatt gagcaactta ctgtaatgaa
     9901 cgcacctaaa ttttacaaac ttgaagacaa tgatacattc attgtccatt cgaaagaaga
     9961 aacagatttt tggctgaaaa cccattatgg ttttgaagta atgaatggcc atgtatttta
    10021 tgatgagata atgacagatt ttcaggctga ggttcaattg cgcatgaatc caaatgcgac
    10081 atacgatcaa gcgggtttat ttgttatgat ttctgagaag tgttggttaa aaacatcttt
    10141 agaatatatt cctgagggcc catcacattt aggtgctgtc gtaacgaata atggctattc
    10201 tgattggtca acgcaagact atccaaatga gtttgctaag caacaattac gttttcgaat
    10261 tattcgaaaa cttggcgatt atacaatata tgtgaaaacg
//
