LOCUS       NZ_CP059991            11440 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptomyces gardneri strain ATCC 15439 chromosome, complete genome.
ACCESSION   NZ_CP059991
VERSION     NZ_CP059991.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces gardneri
  ORGANISM  Streptomyces gardneri
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9054805)
  AUTHORS   Kim,W., Hwang,S. and Lee,N.
  TITLE     Genome-scale analysis on the transcription unit architecture of
            Streptomyces venezuelae ATCC 15439
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9054805)
  AUTHORS   Kim,W., Hwang,S. and Lee,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2020) Biological Sciences, KAIST, 291 Daehak-ro,
            Yuseong-gu, Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 6.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP013129
            Genome Coverage                   :: 187.8x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015710995.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 17:12:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,199
            CDSs (total)                      :: 8,101
            Genes (coding)                    :: 7,918
            CDSs (with protein)               :: 7,918
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 70 of 184
            Pseudo Genes (incomplete)         :: 147 of 184
            Pseudo Genes (internal stop)      :: 16 of 184
            Pseudo Genes (multiple problems)  :: 48 of 184
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:23:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6699792
            Orig. end                         :: 6711232
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059991.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11440
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="23"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11440
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11440
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6440](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6440)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            678..2153
                     /db_xref="GeneID:95692919"
                     /gene="hemG"
                     /locus_tag="H4W23_RS30310"
                     /old_locus_tag="H4W23_30290"
     CDS             678..2153
                     /EC_number="1.3.3.4"
                     /GO_function="GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95692919"
                     /gene="hemG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238123.1"
                     /locus_tag="H4W23_RS30310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_30290"
                     /product="protoporphyrinogen oxidase"
                     /protein_id="WP_055640859.1"
                     /sec_met_domain="DAO (E-value: 4e-11, bitscore: 42.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNADTTGPHVVVAGGGIAGLAAAHRLATAGHRVTLLEADDRLGGK
                     LRAGEIAGAPVDLGAESLLARRPEAVALARTVGLGDRLQAPATTTASVWSRGTLTPMPK
                     GHVMGVPGDGRAVEGLLSEEGVRRIGLDRELPPTEIGDDIAIGAYVSERMGHEVVDRLV
                     EPLLGGVYAGDAYRISMKAAVPALFEAARRHPTLTAAVHAVQQAGATVPADAAGAATGL
                     GGSVFLGVEGGVGTLPGAVADAIRAAGGEILTRTRVTALRRTGPAGWEVVAGDRVLTAD
                     AVVLATPAGPAAALLADHAPAASAELAAIEYASMALITLAFRRADLPGLPGSGFLVPPV
                     DGHTIKASTFSSQKWRWVADGAPDLFVLRTSVGRHGEEEQVHREDTDLVAASLKDLAAA
                     TGLAARPVATTVTRWTGGLPQYPVGHLARVARLREAVAALPGLRVAGAAYDGVGIPACI
                     ASAHRAADEITAEFGDEIIATSKRTDPGAGHSS"
     gene            2171..2884
                     /db_xref="GeneID:95692920"
                     /gene="hemQ"
                     /locus_tag="H4W23_RS30315"
                     /old_locus_tag="H4W23_30295"
     CDS             2171..2884
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /db_xref="GeneID:95692920"
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962204.1"
                     /locus_tag="H4W23_RS30315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_30295"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_055640858.1"
                     /transl_table=11
                     /translation="MSAPEKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPEDRAGYADE
                     VQELFDQLAAKDITVRGTYDLSGLRADADVMIWWHAETSDELQEAYSLFRRTKLGRALE
                     PVWSNMALHRPAEFNKSHIPAFLADENPRDYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGYPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRASEARMHVREEVPFY
                     TGRRKSVADLVAGLA"
     gene            complement(3175..3927)
                     /db_xref="GeneID:95692921"
                     /locus_tag="H4W23_RS30320"
                     /old_locus_tag="H4W23_30300"
     CDS             complement(3175..3927)
                     /codon_start=1
                     /db_xref="GeneID:95692921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238122.1"
                     /locus_tag="H4W23_RS30320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_30300"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_055640857.1"
                     /transl_table=11
                     /translation="MVWVPLLLVACVMAGIGCARLCRASLAAARPTAPSAPEDSELTLL
                     EAAYLSGGPLRVTDLTLVAMHRERRLLLAHTGWATVVDSSTGSRDEWESAVLGAIGPGG
                     QSPIPAVRPAVAAADSVRSLGRRLVDRGLAVPDAGRREIAAGVRAVRAAFLLCLALGTA
                     AALLVPGAERPLVVAAFSLPLLASGLCLLIGRIEVYPYTRWASPAGQRLLSGLSPADPL
                     TALATRGPAVLEPELRAALRGHAPARLP"
     gene            complement(3976..4998)
                     /db_xref="GeneID:95692922"
                     /locus_tag="H4W23_RS30325"
                     /old_locus_tag="H4W23_30305"
     CDS             complement(3976..4998)
                     /codon_start=1
                     /db_xref="GeneID:95692922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238121.1"
                     /locus_tag="H4W23_RS30325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_30305"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_055640856.1"
                     /transl_table=11
                     /translation="MNTLVVVYYVAAAAGIALLITRVVRSRGGPGGPVHDRYEVAFLNG
                     GPARVVDTALAALQYDGRIAIGGPGIVAVVRPVAHDPVERAVLQEHAAAPHGALHHLRL
                     AVMRHPAVQEIGDGLAARGLVVAPSRRRTTQGWCLGLGLTAFFLFPVSVALTMVDLSTA
                     PEMSVPFVVKVLPALFATGVTAIACGAITGRRISPAGQGAVASYGRAHGHLADAGHLVA
                     LYGLRGLPDPVLREQLLAAARVYAPPRGRGRRPAASRHHTTDHAAAAVALEWCADNSFC
                     GGGSSSSDGGSGSCSSGSCSGGGSGCGGGGGSSCSSSSGSSCSSSSSSGSSCSSSSSSG
                     "
     gene            complement(5001..6440)
                     /db_xref="GeneID:95692923"
                     /locus_tag="H4W23_RS30330"
                     /old_locus_tag="H4W23_30310"
     CDS             complement(5001..6440)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95692923"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037016.1"
                     /locus_tag="H4W23_RS30330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_30310"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_055640855.1"
                     /sec_met_domain="DUF692 (E-value: 1.5e-94, bitscore: 315.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTELGIGIGWRPEIAEAVEALPGVDWVEVVAENICADHLPDSLTR
                     LRERGVTVVPHGVSLGLGGADRPDPARLAALGEKAVALGAPLITEHIAFVRAGGPLTAS
                     PLLEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALIAWPGEELTEGQFLA
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAKALAELPVEAIAYVHVAGGVERDGVWHDTH
                     AHPVPGAVLDVLADLASRVSPPGVLLERDDDFPPAEELAAELTVIRQTVAAGGTARPRG
                     ARPSVRVSRRAPLLIGAGARVSGGPMPGAPAGQDLAQKPVSVAEPVSVPGQAPAPVHES
                     APRDGARQRVGLAQAALLSALVAGTPAPEGFDSRRLGVQSRALAAKRAGVVAKVAPELP
                     EILGKGFRPAFLAYARTRPMTGGYRRDALDFAEHLLLADRPEDPAARRALTEWWQDRSG
                     SRPPGRALRFARAARHALARR"
     gene            complement(6561..7358)
                     /db_xref="GeneID:95692924"
                     /locus_tag="H4W23_RS30335"
                     /old_locus_tag="H4W23_30315"
     CDS             complement(6561..7358)
                     /codon_start=1
                     /db_xref="GeneID:95692924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007268528.1"
                     /locus_tag="H4W23_RS30335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_30315"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_055640854.1"
                     /transl_table=11
                     /translation="MADIRTPVQHDEIPVRVLGGPTALFEYGGLRFLTDPTFDAPRDYD
                     VPGARLTKTASASAAPDGLGRIDVVLLSHDEHPDNLDVSGRALLADVPLTLTTPGGGQR
                     LGAGARGLADWESVDLDRPDGGTVTVTGVPALHGPGTREEVEALTGQVVGFVLTGQDLP
                     TLYVSGDNASLDLVREIAERFGPIDTAVLFAGAPRFPQLFGGRLIVLDSAQAAEAAEIL
                     GARRVVPVHCDSWAHFTESRDDVVAAFTKAGLADRLDPDAVPV"
     gene            7444..8043
                     /db_xref="GeneID:95692925"
                     /locus_tag="H4W23_RS30340"
                     /old_locus_tag="H4W23_30320"
     CDS             7444..8043
                     /codon_start=1
                     /db_xref="GeneID:95692925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677186.1"
                     /locus_tag="H4W23_RS30340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_30320"
                     /product="ABATE domain-containing protein"
                     /protein_id="WP_055640853.1"
                     /transl_table=11
                     /translation="MSETSTAESVPPPAPGADEYVALAFVNSAVALPGGHFMDLLGTPA
                     ATNRWLTEHGLAPADAGVQEMCAAQLRSLREHLRSLCAARVAGLPALPTALSAVNDALS
                     KAPTAALLYWDEKNGPYRAAPCPTDQILGRALATLAANAADLLAGPDADRLTSCGSTPC
                     NRYLLRHGRRHWCSTRCGDRARAARAYARRTRPQTD"
     gene            complement(8086..8475)
                     /db_xref="GeneID:95692926"
                     /locus_tag="H4W23_RS30345"
                     /old_locus_tag="H4W23_30325"
     CDS             complement(8086..8475)
                     /codon_start=1
                     /db_xref="GeneID:95692926"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H4W23_RS30345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H4W23_30325"
                     /product="hypothetical protein"
                     /protein_id="WP_055640852.1"
                     /transl_table=11
                     /translation="MSLLFLFLLAGVGARAGRPGRRKPRRIATSDEVRIERERAEHELR
                     ARAEEIGRTSPWGPAVRTRLVDTCARGGGRNHLARLPEPRSARRAVFGTCVSDPSDVTL
                     AALRERPGTLFAWTLSTMYHTVPGA"
     gene            complement(8537..9250)
                     /db_xref="GeneID:95692927"
                     /locus_tag="H4W23_RS30350"
                     /old_locus_tag="H4W23_30330"
     CDS             complement(8537..9250)
                     /codon_start=1
                     /db_xref="GeneID:95692927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049034.1"
                     /locus_tag="H4W23_RS30350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_30330"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_055640851.1"
                     /transl_table=11
                     /translation="MSSEETDVRDEKPQFVLPLVVRMERDAPPTRTDALETAARAVMEI
                     LTDDRSLGEGEWAQAMTDWEDARIRKVVRRARGAEWRRAGTLPGIAVTGAEAEVRVFPP
                     VPLDGWPKELAKLQVSGTDLDDPAEPGPVPDGAVVLWINPGVDMSAGKTMAQAGHGAQL
                     LWWAMPDADRKAWQEAGFPLAVRTATPERWAALAASGSGLPVVRDAGFTEIAPGKTVVS
                     EGSHRFAPHLRLRQA"
     gene            9396..10244
                     /db_xref="GeneID:95692928"
                     /locus_tag="H4W23_RS30355"
                     /old_locus_tag="H4W23_30335"
     CDS             9396..10244
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95692928"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020477442.1"
                     /locus_tag="H4W23_RS30355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_30335"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_055640850.1"
                     /transl_table=11
                     /translation="MRLERVGRRYGPRGPWVLRGADLDLPGSALVRVVGTNGTGKSTLL
                     RLVAGIDAPSEGRITGRPPRTAYVPERFPAALPFTATDYLVHLGRVHGLGRATARARAG
                     EWLERFGAGEHARTELAELSKGTGQKVAVAQALLADPDLLVLDEAWTGLDTTARTELDR
                     AVRERVAAGATAVFVDHDPRRLAGEADAVYGVESGTVRAVAATARDVPKEPRVRITASG
                     GKPLPEGLPGTPAVEPPDPDDPARPLVLTVDAAHSDALLGALLAASWHIHHLGTEEGVR
                     V"
     gene            10241..10891
                     /db_xref="GeneID:95692929"
                     /locus_tag="H4W23_RS30360"
                     /old_locus_tag="H4W23_30340"
     CDS             10241..10891
                     /codon_start=1
                     /db_xref="GeneID:95692929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238116.1"
                     /locus_tag="H4W23_RS30360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_30340"
                     /product="hypothetical protein"
                     /protein_id="WP_055640849.1"
                     /transl_table=11
                     /translation="MTALLRYQSGLLLRSQRWLAPLLLYAAVLAVGVRAGEPVLGALGF
                     SAAALVPVTAWCVRICLTQEPPAARDVVAAAAGRGRAHLAALLTGTALPGLLGTVAVLG
                     LTAIGDRRGVGALAATGAGLLSTAACVLTGAAVGALAARPFLKAAGWSVTALVTGSLLA
                     LVTTGSPAQHALTALVTGSRTTTAHLPWLACAGAVLLAAATAALACRATARLE"
     gene            10921..11112
                     /db_xref="GeneID:95692930"
                     /locus_tag="H4W23_RS30365"
                     /old_locus_tag="H4W23_30345"
     CDS             10921..11112
                     /codon_start=1
                     /db_xref="GeneID:95692930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019762318.1"
                     /locus_tag="H4W23_RS30365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_30345"
                     /product="hypothetical protein"
                     /protein_id="WP_055640848.1"
                     /transl_table=11
                     /translation="MTDATSYCGIPEPAGPKPEPAAEGPFASCVLCQEPTEYPESTKGI
                     TLCPVCEWQEAQRTACSG"
ORIGIN
        1 cccgctgggt ggcgatccgg ctctccacgt ccaccacctg ctcggtgacg tccttggcgt
       61 cggccttgcg ggcgaggagc ttcccggtgc ccgccagctc ggtgagcacc gcctcgtact
      121 gctcctgcgg cacccgcagc accacgcgcg agacgacgta cgtgtcgtcg atgcgctccg
      181 tcgactcgtt ctccacccgc ccgccggcgc ccgccgccac cctgcgggcc gtgaccaggg
      241 ccttcgccgc gtcctcgacc tccacgctga gcgaggcggt acggatgacg tgctgctgcc
      301 gcacggcggg cttgcccggc gtcgcggggg cgcgctcggc ggccgaggcg gcgtccgccg
      361 ggccctgcgc ggccttggcc ccgcccgcgt cggcgggctg cgccgccaga tcgcgcttgc
      421 tgtcgccgcc gtggtccgcc gagccgccgg cgcagccggt caggacgagc gaggccgtca
      481 gcagcgcggc ggcggccgcc gtccgacccc gtgacgccgt gcgtgttccg ttcatgagtt
      541 ccccccaggg aatggtggcg agtcgcgtgc cccttggacg tgggagtcct cgatacgggt
      601 ttccctccgg cggtagcgat gcggtcacgg tccggcctcg gaacggaggc gcgtgggcga
      661 tttgagagag tggtcccatg aacgcggaca ccacgggacc ccacgtcgtc gtcgccggag
      721 gcggaatcgc cggactcgcg gccgcccacc ggctcgccac cgccggccac cgggtgaccc
      781 tcctcgaagc ggacgaccgc ctcggcggca aactccgcgc cggggagatc gcgggcgcgc
      841 ccgtggacct cggcgccgag tcgctgctcg cccgccgccc cgaggccgtc gccctcgccc
      901 ggaccgtcgg cctcggcgac cggctccagg caccggccac gaccaccgcc tccgtctggt
      961 cccgcggcac gctcaccccc atgcccaagg gccatgtgat gggcgtcccc ggcgacggcc
     1021 gcgcggtcga ggggctcctc tccgaggagg gcgtgcgccg gatcggcctc gaccgcgagc
     1081 tccccccgac cgagatcggc gacgacatcg ccatcggcgc gtacgtctcc gagcgcatgg
     1141 gccacgaggt cgtcgaccgc ctcgtcgaac ccctcctcgg cggcgtctac gccggtgacg
     1201 cctaccgcat ctcgatgaag gccgctgtcc ccgccctctt cgaggccgcc cgccgccacc
     1261 ccacgctcac cgccgccgtc cacgccgtcc agcaggccgg ggccaccgtc ccggccgacg
     1321 cggcgggcgc cgccaccggc ctcggcggct ccgtcttcct cggcgtcgag ggcggtgtcg
     1381 gcaccctgcc cggcgccgtc gccgacgcga tccgcgccgc cggcggcgag atcctcaccc
     1441 gtacccgcgt caccgccctg cgccgcaccg gaccggccgg ctgggaggtc gtcgccgggg
     1501 accgggtcct gaccgccgac gccgtcgtcc tcgccacccc cgccgggccc gccgcagcgc
     1561 tcctcgccga ccacgcgccc gccgcctccg ccgagctcgc cgccatcgag tacgcctcca
     1621 tggcgctgat cacgctcgcc ttccgccgcg ccgacctgcc cggcctgccg ggctcgggtt
     1681 tcctcgttcc ccccgtcgac ggccacacca tcaaggcctc caccttctcc agccagaagt
     1741 ggcggtgggt cgccgacggc gcccccgacc tcttcgtgct gcgcacctcc gtcggccgcc
     1801 acggcgagga ggagcaggtc caccgcgagg acaccgacct cgtcgccgcc tccctcaagg
     1861 acctcgccgc cgccaccggg ctcgccgcgc gccccgtcgc caccacggtc acccgctgga
     1921 ccggcggcct gccccagtac ccggtcggcc atctcgcccg ggtcgcccgc ctccgcgagg
     1981 ccgtcgccgc cctgcccgga ctgcgggtcg cgggcgccgc gtacgacggc gtcggcatcc
     2041 ccgcgtgcat cgcctcggcg caccgcgcgg ccgacgagat caccgccgag ttcggggacg
     2101 agatcatcgc cacgtcgaaa aggaccgacc ccggtgcggg gcactcctcg tgacgggcga
     2161 gaataggcgt atgagtgcgc ccgaaaagat cccgaacgcc ggtaagaagg cgaaggacct
     2221 caacgaggtc atccgctaca ccctgtggtc tgtgttcaag ctgcgcgatg ttctgcccga
     2281 ggaccgcgcc ggctacgccg acgaggtcca ggagctgttc gaccagctcg ccgccaagga
     2341 catcaccgtc cgcggcacgt acgacctctc cggtctgcgc gccgacgccg acgtcatgat
     2401 ctggtggcac gccgagacga gcgacgagct ccaggaggcg tacagcctct tccgccgtac
     2461 caagctgggc cgcgccctgg agccggtctg gtccaacatg gcgctgcacc gccccgccga
     2521 gttcaacaag tcgcacatcc cggccttcct ggccgacgag aacccgcgcg actacgtctc
     2581 ggtctacccc ttcgtgcgct cctacgactg gtacctgctg ccggacgagg accgtcgccg
     2641 catgctcgcc gaccacggca agatggcccg cggctacccc gacgtccgcg ccaacacggt
     2701 cgcctccttc tcgctcggcg actacgagtg gatcctcgcc ttcgaggccg acgagctgca
     2761 ccgcatcgtc gacctcatgc gccacctgcg tgcctccgag gcccggatgc acgtccgcga
     2821 ggaggtcccg ttctacaccg gtcgccgcaa gagcgtcgcc gacctggtgg cgggcctggc
     2881 ctgacacggc cgtacgaacc tggtggcggc ccggctcccg gccgggcgcg ccaccccttg
     2941 acccggaaga tcagacggcg ggcgtcttca ctgccctccg ctccagcgac accgggtccg
     3001 gctccgggtg cggcgcgcac gacgcgcgcc gcaccggcgt catttccggg cccgtgacga
     3061 ctccgggtac cgcccccgtg agcagagctc ccgcgtacgg ggccgccgcg tcccgccccg
     3121 ccgccaggat cagcgaagag atcgtcggcg cggcggagga gcgaagtgaa ggatctacgg
     3181 gaggcgcgcg ggagcgtgtc cccgaagtgc cgcgcggagc tcgggctcca ggacggcggg
     3241 gccgcgcgtc gccagcgccg tgagcggatc ggcgggggag aggccggaga gcagccgctg
     3301 ccctgcgggg gacgcccagc gggtgtacgg gtacacctcg atccggccga tcagcagaca
     3361 gagccccgag gcgagcagcg gcagcgagaa cgcggcgacg accaggggcc gttcggcgcc
     3421 gggcacgagg agtgccgccg ccgtcccgag cgccaggcag agcaggaagg cggcccgcac
     3481 cgcccgtacc ccggctgcga tctcccggcg gcccgcgtcg gggacggcga gcccccggtc
     3541 gacgagccgc cggccgagcg accgcactga atcggcggcg gccaccgcgg gacgtaccgc
     3601 ggggatcggg gactggccgc cgggcccgat ggcgccgagc acggcgctct cccactcgtc
     3661 ccgggagccg gtggaggagt ccacgaccgt cgcccagccg gtgtgggcga ggagcagccg
     3721 ccgctcccgg tgcatcgcga cgagggtgag gtcggtgacg cggagcggac cgccggagag
     3781 ataggcggct tcgaggaggg tgagctcgga gtcctccggg gcggacgggg ccgtcggccg
     3841 ggccgccgcg agggaggccc ggcacagccg tgcgcagccg atgccggcca tgacgcacgc
     3901 cacgaggaga agcggaaccc agaccatgtc cgagttctac gacaggacgg gcgggcccgc
     3961 cactcgaacg ggtgatcagc cgctggaact ggagctggag cacgaactcc cgctggaact
     4021 ggagctggag cacgagctgc cgctggagct cgaacaggaa ctgccgcctc cgcctccgca
     4081 gcccgacccg ccgcccgagc acgaaccgga ggagcaggaa ccggagcctc cgtcggaaga
     4141 ggaggagccg ccgccgcaga agctgttgtc cgcgcaccat tcgagggcca ccgcggcggc
     4201 ggcgtggtcc gtggtgtggt gacgcgaggc cgcgggccgc cgcccacgcc cccgcggagg
     4261 cgcgtacacc cgggcggcgg cgaggagttg ctcccgcagc accgggtcgg gcagcccccg
     4321 cagcccgtac agggccacga ggtgaccggc atccgcgagg tgaccatggg cccgtccgta
     4381 cgaggcgacg gccccctgtc cggccgggct gatccgccgt ccggtgatcg ccccgcacgc
     4441 gatggcggtg accccggtgg cgaagagggc gggcagcacc ttcacgacga acggcaccga
     4501 catctccggt gcggtggaga gatcgaccat cgtgagggcg accgagacgg ggaagaggaa
     4561 gaaggcggtg agaccgagcc cgaggcacca gccctgggtg gtgcgccgtc tcgacggcgc
     4621 gacgacgagt ccgcgtgccg cgagcccgtc accgatctcc tgcaccgcgg ggtgccgcat
     4681 cacggcgagc cgcaggtggt gcagggcccc gtgcggggcc gccgcgtgct cctggagcac
     4741 ggcccgctcg accgggtcgt gcgcgacggg ccggacgacc gcgacgatgc cggggccgcc
     4801 gatcgcgatc ctgccgtcgt actggagggc ggcgagggcg gtgtcgacga cccgggccgg
     4861 gccgccgttc aggaaggcga cctcgtaccg gtcgtggacc ggtccgccgg ggccgccgcg
     4921 cgaccggacg acccgggtga tcagcagcgc gatgccggct gcggcggcca cgtagtagac
     4981 gacgaccaag gtgttcacgg tcacctcctg gcgagggcgt ggcgggcggc tcgggcgaac
     5041 cggagggcgc ggccgggcgg acggctgccg gagcggtcct gccaccactc cgtgagcgcc
     5101 cgtcgggccg ccgggtcctc ggggcggtcg gcgagcagca ggtgctcggc gaagtcgagg
     5161 gcgtcacggc ggtagccgcc ggtcatgggg cgggtcctgg cgtacgcgag gaacgcgggc
     5221 cggaagccct tgccgaggat ctccggcagc tcgggggcga ccttcgcgac gaccccggcc
     5281 cgcttggcgg cgagggcccg gctctgcacc ccgaggcgcc ggctgtcgaa cccctcgggc
     5341 gcgggcgtcc cggcgacgag cgccgacagc agcgcggcct gcgcgagccc gacccgctgc
     5401 ctggcgccgt cccgcggcgc ggactcgtgt acgggagcgg gcgcctgccc gggcacggac
     5461 acgggctccg ccacggacac gggcttctgc gcgaggtcct gcccggccgg agcgcccggc
     5521 atcggcccgc ccgacacacg ggcaccggcg ccgatgagca gcggcgcccg tcgggacacg
     5581 cggacggacg gacgggcgcc gcgcgggcgg gccgtccccc cggcggccac cgtctgccgg
     5641 atcaccgtca gctccgccgc cagctcctcc gccggaggga agtcgtcgtc ccgctccagc
     5701 agcacccccg gtggcgagac ccgcgacgcg aggtccgcca ggacgtcgag aacggccccc
     5761 ggcaccgggt gcgcgtgggt gtcgtgccag accccgtccc gctcgacacc gcccgccacg
     5821 tgcacgtacg cgatggcctc caccggcagc tcggccaggg ccttcgccgg gtcctcgccg
     5881 cggttgacgt ggttggtgtg caggttggcg acgtcgatga ggagccgtac ccccgtacgc
     5941 tccaccagct ccgccaggaa ctgcccctcc gtcagctcct caccgggcca ggcgatcagg
     6001 gccgcgatgt tctccagggc gagcggtacg ggcagcgcgt cctgggcgat gcgcacgttc
     6061 tcgcagagca cgtcgagcgc gtcccgcgtc cggggcacgg gcagcaggtg gcccgcttcg
     6121 agcagcggcg aggcggtgag cggccccccg gcccgtacga acgcgatgtg ctccgtgatc
     6181 agcggcgctc ccagcgccac ggccttctcc ccgagcgcgg cgagccgcgc cgggtccggc
     6241 cggtccgcgc cgccgagccc cagcgacacc ccgtgcggga cgaccgtcac gccgcgctcg
     6301 cgcagccggg tcagcgagtc gggcaggtgg tcggcgcaga tgttctccgc gaccacctcg
     6361 acccagtcga cgccgggcag cgcctccacc gcctccgcga tctcgggacg ccagccgatg
     6421 ccgatgccga gttccgtcat gtccccctcc ttcggtcctc agtcatccac gtgatgaccc
     6481 gtggagggac gttcgaatcc ggagaggggg acgttcagag ctacatttga ggttccggcc
     6541 tcagatggct tcagatggcc tcagacgggg accgcatcgg ggtccagccg gtcggccagc
     6601 ccggccttcg tgaacgcggc gaccacgtcg tcacggctct cggtgaagtg cgcccagctg
     6661 tcgcagtgga cgggaaccac gcgccgggcg ccgaggatct ctgccgcctc cgcggcctgt
     6721 gcgctgtcga ggacgatcag ccggccgccg aagagctggg ggaagcgcgg cgctccggcg
     6781 aagagcacgg cggtgtcgat cgggccgaac cgttcggcga tctcccggac caggtcgagc
     6841 gaggcgttgt cgccgctgac gtagagggtg ggcaggtcct gcccggtcag gacgaagccg
     6901 acgacctggc ccgtgagggc ctcgacctcc tcgcgggtgc cggggccgtg gagggcgggc
     6961 acgccggtga cggtgacggt gccgccgtcg gggcggtcca ggtcgaccga ctcccagtcg
     7021 gccagccccc tggcccccgc ccccaggcgc tgtccgccgc cgggcgtggt cagggtcagc
     7081 gggacgtccg cgagcagggc ccggccggac acgtcgaggt tgtcggggtg ctcgtcgtgg
     7141 gagagcagga ccacgtcgat acggccgagg ccgtcggggg cggcggaggc ggaggccgtc
     7201 ttggtcagcc gggcgccggg cacgtcgtag tcgcggggcg cgtcgaaggt gggatcggtc
     7261 aggaagcgca ggccgccgta ctcgaagagg gccgtcgggc ctccgaggac gcggacgggg
     7321 atctcgtcgt gctgtacggg cgtgcggatg tcagccatca gaacctcacg aataggtagt
     7381 ggtctatccg tgaggaccat agcccatgac tcacggatga gcgcaagccg taccatgaca
     7441 accatgagtg agacctcgac cgccgaatcc gtgccgccgc ccgcaccggg ggcggacgag
     7501 tacgttgccc tcgccttcgt caacagtgcc gtcgcgctgc ccggggggca cttcatggat
     7561 ctcctcggga cccccgcggc cacgaaccgg tggctcaccg agcacggcct cgcgccggcc
     7621 gacgccgggg tgcaggagat gtgcgcggcg cagctgcgct ccctgcgcga acacctcagg
     7681 tcgttgtgcg cagcccgggt cgcggggctg cccgccctgc ccacggcgct ctccgcggtc
     7741 aacgacgcgc tgagcaaggc ccccacggcc gccctgctgt actgggacga gaagaacggc
     7801 ccgtaccgtg cggcgccctg ccccaccgac cagatcctcg gccgggctct cgcgactctc
     7861 gcggccaacg ccgccgacct gctcgccggc cccgatgccg accgcctcac cagctgcggc
     7921 tccaccccct gcaaccgcta cctgctgcgc cacggccgcc ggcactggtg ctccacccgc
     7981 tgcggcgacc gcgcgcgcgc agcccgcgcc tacgcccgcc gcacccggcc gcagacggac
     8041 tgacggtgac ggtggccgtg acggggacct gccggatcgc ccccgctacg cgcccgggac
     8101 ggtgtggtac atcgtggaca gcgtccacgc gaacaaggtg cccgggcgct cccgcagcgc
     8161 ggcgagggtg acgtcggagg ggtcggacac gcaggtgccg aagaccgccc tgcgcgcgga
     8221 gcgcggctcc gggagcctcg cgaggtggtt cctgcccccg ccccgggcac acgtgtcgac
     8281 cagcctggtg cgcacggccg gaccccacgg cgaagtgcgg ccgatctcct cggcgcgcgc
     8341 ccgcagttcg tgctcggcgc gctcacgctc gatccggacc tcgtccgacg tggcgatcct
     8401 cctgggcttc cgcctgccgg gccggccggc gcgggcaccg accccggcca ggaggaagag
     8461 gaacagcagg gacacggccc ggtccgctcg ccggccgacc cgccggaccg cacgcggcgt
     8521 aatgcgccga gcccccctac gcctgccgca gccgcaggtg aggagcgaag cggtggctcc
     8581 cctcggacac caccgtcttt cccggcgcga tctccgtgaa gccggcgtcc cgcacgacgg
     8641 gcagcccgct gccgctcgcc gcgagcgcgg cccagcgctc gggcgtcgcg gtgcgcacgg
     8701 cgagcgggaa gcccgcctcc tgccaggcct tccggtccgc gtccggcatc gcccaccaca
     8761 gcagctgcgc gccgtgcccc gcctgcgcca tcgtcttgcc cgccgacatg tcgactccgg
     8821 gattgatcca gagcaccacg gccccgtccg gcacgggacc gggctcggcc gggtcgtcga
     8881 ggtcggtgcc cgacacctgg agcttggcca gctccttggg ccagccgtcg agcggcaccg
     8941 gcgggaagac ccgtacctcc gcctccgcgc cggtcacggc gatccccggg agcgtcccgg
     9001 cccgccgcca ctcggcaccg cgcgcccggc gcacgacctt gcggatccgg gcgtcctccc
     9061 agtccgtcat ggcctgcgcc cactcgccct caccgaggga acggtcgtcg gtgaggatct
     9121 ccatgacggc ccgcgcggcg gtctccaggg cgtcggtgcg ggtcgggggc gcgtcgcgct
     9181 ccatccgcac caccaagggg aggacgaact ggggcttctc atcgcggacg tcggtctctt
     9241 cgctgctcac ccggcaagtc tgccaccgcg gcccggagcg aatcttgtgg gaaccatcgc
     9301 ttccgggtca ggatgcccgg catgacgagc gatcttcccg ccggcgaaca ccccgcaggg
     9361 acggccggct tcccgggcac gaccctccgg aaccggtgag gctggaacgg gtcgggcgcc
     9421 ggtacggtcc gcgcgggccg tgggtcctgc gcggggccga cctcgacctg cccggctccg
     9481 cgctcgtccg ggtcgtcggc accaacggca ccggcaagtc gacgctgctc cgcctggtcg
     9541 ccgggatcga cgccccgagc gagggccgga tcaccggccg cccgccccgc acggcgtacg
     9601 tccccgagcg cttccccgcg gccctgccct tcacggccac cgactacctg gtccacctgg
     9661 gccgcgtcca cggcctcggc cgggcgacgg ccagggcccg ggcgggggag tggctggagc
     9721 gcttcggggc cggcgagcac gcccgtacgg aactggcgga actctccaag ggcaccggcc
     9781 agaaagtcgc cgtcgcccag gccctcctcg ccgaccccga cctcctcgtc ctcgacgagg
     9841 cctggaccgg cctcgacacc acggcccgca ccgaactcga ccgcgccgtc cgggaacgcg
     9901 tcgcggccgg cgccaccgcc gtcttcgtcg accacgaccc gcgccgcctc gcgggggagg
     9961 cggacgcggt gtacggcgtg gagtcgggga cggtacgggc cgtcgcggcc acggcgcggg
    10021 acgtaccgaa ggagccgcgg gtccggatca ccgcctccgg cggcaagccg ctcccggagg
    10081 gcctgccggg cacccccgcc gtcgagccgc cggacccgga cgaccccgcc cgccctctcg
    10141 tcctgaccgt ggacgcggcg cactccgacg ccctgctcgg ggcgctcctc gcggcctcct
    10201 ggcacatcca ccacctcggc acggaagagg gcgtacgggt atgacggcgc tcctgcgcta
    10261 ccagtcgggt ctgctgctcc gctcgcagcg ctggctcgca cccctcctgc tctacgcggc
    10321 cgtcctcgcc gtcggcgtcc gcgcgggcga acccgtgctc ggcgccctcg gcttctcggc
    10381 ggcggccctg gtgccggtga cggcctggtg cgtacggatc tgcctcaccc aggaaccgcc
    10441 cgccgcccgc gacgtggtcg cggcggccgc cggccggggc cgcgcccacc tcgcggcgct
    10501 cctcaccggg accgcgctgc cgggactcct cggcacggtc gccgtcctcg gtctcaccgc
    10561 gatcggcgac cggcgcgggg tcggcgcgct cgccgccacg ggcgccgggc tgctctcgac
    10621 ggcggcctgt gtcctgacgg gcgccgcggt cggcgcgctg gccgcccgcc cgttcctcaa
    10681 ggccgccggc tggtcggtca cggccctcgt caccggctcg ctcctcgccc tcgtcaccac
    10741 gggctccccc gcgcagcacg ccctgacggc cctggtcacc ggctcccgca cgacgaccgc
    10801 gcacctgccc tggctcgcct gcgcgggtgc cgtgctcctc gcggcggcga cagctgccct
    10861 cgcctgccgg gccacggcac gcctggagtg agcggaaccg aggggcgtac gctcgaagac
    10921 atgaccgatg cgacgtcgta ctgcggcatc cccgagcccg ccggcccgaa gcccgaaccg
    10981 gcggccgagg gccccttcgc ctcctgcgtg ctgtgccagg agccgaccga gtacccggag
    11041 tcgaccaagg ggatcacgct ctgcccggtc tgcgagtggc aggaggccca gcgcacggcc
    11101 tgctcgggct gaagggcccg gcgttccagc cgttccccat cagctcggag acatcagctc
    11161 ggagaccttc acgaagcggt agccgcgcgc ccgcagctcg gggacgatcc ggcggatcgc
    11221 ctgctcggtg acgggggcgg cgctgcgcgt gcagtgcatg acgaccagcg aaccgggctt
    11281 caccccgtcg aggacctgct cggcgacggc gtccgcgtcc ttggcgaagg cgtcgccgct
    11341 gaccacgtcc cactggaccg cggtcacccc ggcggggccg agcgcgcgca gggccgcgtc
    11401 gtcgtagcac ccgccgggga agcggaagta cgggacgacg
//
