LOCUS       NZ_CP059991            10414 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptomyces gardneri strain ATCC 15439 chromosome, complete genome.
ACCESSION   NZ_CP059991
VERSION     NZ_CP059991.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces gardneri
  ORGANISM  Streptomyces gardneri
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9054805)
  AUTHORS   Kim,W., Hwang,S. and Lee,N.
  TITLE     Genome-scale analysis on the transcription unit architecture of
            Streptomyces venezuelae ATCC 15439
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9054805)
  AUTHORS   Kim,W., Hwang,S. and Lee,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2020) Biological Sciences, KAIST, 291 Daehak-ro,
            Yuseong-gu, Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 6.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP013129
            Genome Coverage                   :: 187.8x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015710995.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 17:12:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,199
            CDSs (total)                      :: 8,101
            Genes (coding)                    :: 7,918
            CDSs (with protein)               :: 7,918
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 70 of 184
            Pseudo Genes (incomplete)         :: 147 of 184
            Pseudo Genes (internal stop)      :: 16 of 184
            Pseudo Genes (multiple problems)  :: 48 of 184
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:23:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5666586
            Orig. end                         :: 5677000
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059991.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10414
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="19"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10414
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10414
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5414](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5414)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    complement(65..78)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.42"
                     /tool="antismash"
     misc_feature    84..95
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 16.02"
                     /tool="antismash"
     gene            304..543
                     /db_xref="GeneID:95692017"
                     /locus_tag="H4W23_RS25770"
                     /old_locus_tag="H4W23_25750"
     CDS             304..543
                     /codon_start=1
                     /db_xref="GeneID:95692017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="H4W23_RS25770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_25750"
                     /product="chaplin"
                     /protein_id="WP_055646045.1"
                     /transl_table=11
                     /translation="MSRIAKAVVLTVGAAAAVAGAAGVAAADSIAEGAAVKSPGVGSGN
                     VVQVPVHIPVNVCGNTVSVIGALNPTFGNFCANV"
     gene            820..1773
                     /db_xref="GeneID:95692018"
                     /locus_tag="H4W23_RS25775"
                     /old_locus_tag="H4W23_25755"
     CDS             820..1773
                     /codon_start=1
                     /db_xref="GeneID:95692018"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H4W23_RS25775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H4W23_25755"
                     /product="hypothetical protein"
                     /protein_id="WP_055641597.1"
                     /transl_table=11
                     /translation="MRPLATRRITALAISAAITLGAAGPAVAGEHHPSSAADRAAHAPL
                     PEAAALLTQAEALGDLGSVTTPVTELVTAALKADAGQLPAADADALKTKIAAAVEQAKE
                     TAPAPLPDTPAPDLPAPDLPSPDLPAPDLPSPDLPAPDLPAPDLPAPDLPAPDLPAPDL
                     PAPDLPAPDLPAPDLPAPDLPVKPPVSLPVAAGAKAASAAPADVVDDALATVEKAVAGL
                     LAAVTSGDAAGVVPQVTATLTSLVNLAVATVLGSGLPAPDLAGLPALPALPVAVPELPV
                     DPPELPVDPPELPVDAPVKPPVGLPDLPVKPPLPVR"
     gene            1998..2264
                     /db_xref="GeneID:95692019"
                     /locus_tag="H4W23_RS25780"
                     /old_locus_tag="H4W23_25760"
     CDS             1998..2264
                     /codon_start=1
                     /db_xref="GeneID:95692019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035834.1"
                     /locus_tag="H4W23_RS25780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_25760"
                     /product="hypothetical protein"
                     /protein_id="WP_055641596.1"
                     /transl_table=11
                     /translation="MKSTKVAAIVAGSLVALGAAAPAMATEALTPTSLNGGLDAIAANG
                     LKTDMLSSTTDGSPVKTATDTLGQLNEAGKGGSALLGGLPLGG"
     gene            2424..2876
                     /db_xref="GeneID:95692020"
                     /locus_tag="H4W23_RS25785"
                     /old_locus_tag="H4W23_25765"
     CDS             2424..2876
                     /codon_start=1
                     /db_xref="GeneID:95692020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H4W23_RS25785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H4W23_25765"
                     /product="hypothetical protein"
                     /protein_id="WP_057950504.1"
                     /transl_table=11
                     /translation="MRSIISRKTLTAAAAAGVLSLTGGFALAANSHAGTGDSPGPGSAG
                     HVQGPLPADVCGKDGELGELIAAAVGDLCVEAGEPGGYGDEPTHPPTTQPPTTHPPTTQ
                     PPTTTPPELPDTGGDSALMGAAAISAALIIGGTVVYLRGGRISRRH"
     gene            2995..3507
                     /db_xref="GeneID:95692021"
                     /locus_tag="H4W23_RS25790"
                     /old_locus_tag="H4W23_25770"
     CDS             2995..3507
                     /codon_start=1
                     /db_xref="GeneID:95692021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035837.1"
                     /locus_tag="H4W23_RS25790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_25770"
                     /product="hypothetical protein"
                     /protein_id="WP_055641595.1"
                     /transl_table=11
                     /translation="MTLRSHAGMGILMTALASAALAAPASAVEAPVIVPLQGLEPVLPM
                     DAPTVATGVPVPVPGAPTGFQKGLGSLPDLALPSVPLTGTLPKTVVDAPLPEVLDGSDP
                     GRALLSSPHSEMEAATPGAVVGNPVEAPRGNGLAGLPDLAKPQVGVLTPVLSGALDPQL
                     GLVPTSR"
     gene            3797..4084
                     /db_xref="GeneID:95692022"
                     /locus_tag="H4W23_RS25795"
                     /old_locus_tag="H4W23_25775"
     CDS             3797..4084
                     /codon_start=1
                     /db_xref="GeneID:95692022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035838.1"
                     /locus_tag="H4W23_RS25795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_25775"
                     /product="chaplin"
                     /protein_id="WP_055641594.1"
                     /transl_table=11
                     /translation="MSRIAKAAAVLAGTGAVALSGAGLAVADSGAEAVAAHSPGVASGN
                     VVQVPVHVPVNLCGNTVNVIGLLNPAFGNTCINADGHGHGHDNGDGGYGG"
     gene            complement(4151..5017)
                     /db_xref="GeneID:95692023"
                     /locus_tag="H4W23_RS25800"
                     /old_locus_tag="H4W23_25780"
     CDS             complement(4151..5017)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95692023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239814.1"
                     /locus_tag="H4W23_RS25800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_25780"
                     /product="tyrosinase family protein"
                     /protein_id="WP_055641593.1"
                     /transl_table=11
                     /translation="MVYTRKNQRDLTGAERRKFVAAVLELKRTGAYDEFVRTHIDHYTA
                     DGEGRLRTAHMAPSFLPWHRRFLLEFEKALRRIDGTVSVPYWDWTRDNSPASSIWGEDF
                     MGGNGRRADLQVMTGPFAHSSGRWNITYAMSETRFLTRDFGRPRDPVALPTQAQLEEVM
                     RERVYDVAPWNSTSASGFRNRLEGWASGQGNERFRIHNRVHRWVGGLMLGGGSVNDPVF
                     WLHHSFVDLVWSRWQQRNPSAAYLPAEPPPLGDAQYRKVVARHQPMAPWGVTPAELFDH
                     SRIYRYA"
     gene            complement(5001..5414)
                     /db_xref="GeneID:95692024"
                     /locus_tag="H4W23_RS25805"
                     /old_locus_tag="H4W23_25785"
     CDS             complement(5001..5414)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95692024"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239815.1"
                     /locus_tag="H4W23_RS25805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_25785"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_055641592.1"
                     /sec_met_domain="melC (E-value: 9.4e-41, bitscore: 138.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVPRRVALRSLFALAVTAFTGGALGRIATRTPPGAPSGAPHDPL
                     AFDEMYGGRRIRGFSGPGGRPVALVDGRPLHLMRCADGGYVSPIDHYESCPTPLAATRA
                     AVDELGTAPLSRFAAGHGVHTEPTGGSPRGVHA"
     gene            5564..6067
                     /db_xref="GeneID:95692025"
                     /locus_tag="H4W23_RS25810"
                     /old_locus_tag="H4W23_25790"
     CDS             5564..6067
                     /codon_start=1
                     /db_xref="GeneID:95692025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889043.1"
                     /locus_tag="H4W23_RS25810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_25790"
                     /product="DUF5949 family protein"
                     /protein_id="WP_230883108.1"
                     /transl_table=11
                     /translation="MTTSNVVSSARSASPLGTLTVIPWSSEPSADEAGTPFLMAYSLGD
                     GRDGPEAGQQAMRTALESMGLSVGDRLFDLEKDAGINATLLVEGGSAVLNLPFLKVQCP
                     VPDEWQAAARQRGQVYLICSVRPWPEAVPGQPVDEARLRTFVSDEEMLAESAHVLMPVR
                     RIQG"
     gene            6095..6745
                     /db_xref="GeneID:95692026"
                     /locus_tag="H4W23_RS25815"
                     /old_locus_tag="H4W23_25795"
     CDS             6095..6745
                     /codon_start=1
                     /db_xref="GeneID:95692026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239817.1"
                     /locus_tag="H4W23_RS25815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_25795"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_055641590.1"
                     /transl_table=11
                     /translation="MATVNVQGGVPGQRQEESAPGAAGTGLGTGRAFAWMLMVTGAAGV
                     LAGWVITIDKFKLLEDPGFQPGCSLNPVVSCGNIMKSEQAAVFGFPNPMLGLVTYAVVV
                     AVGAGLLSGARYRGWFWLGLNAGMLFGVGFCTWLMYQSLYEINALCLWCCLAWTATIVM
                     FWYVTAHNVRAGLLPAPAGVRTFLGEFAFALPVLHIGIIGMLILTRWWDFWTS"
     gene            6747..7763
                     /db_xref="GeneID:95692027"
                     /locus_tag="H4W23_RS25820"
                     /old_locus_tag="H4W23_25800"
     CDS             6747..7763
                     /codon_start=1
                     /db_xref="GeneID:95692027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015035843.1"
                     /locus_tag="H4W23_RS25820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_25800"
                     /product="glycoside hydrolase family 26 protein"
                     /protein_id="WP_055641589.1"
                     /transl_table=11
                     /translation="MNPFLLWPRAALIVLLAAVLVLLLSHDGIAPGGRHTGSRAPVAPE
                     PLPGVPSGFFTGSDEAGVRRIAQVQAWLGGETLTVGHTYLPGDRWSNIEGHPALFEPWA
                     RWKEERPGRLFVLNVPLLDRNEAGLPDTEVRAGLRRGAGGAFDAHFRVLGERLVEHGLD
                     DAVLVLGWEMNGTTYSHRCGPDPTAWKAYWRRVVGVLRDVAGQRFRFDFTPSRGLDAVA
                     WPLCYPGDDVVDIIGMDAYDQPAGVSFEEQVTEEYGLEHHVRFAAAHGKPVSYPEWGLF
                     RNGDNPVYVRGMLDWFAAHRPVYQTVTDYCPHGVWECADNPESARIVRGALGAPARP"
     gene            complement(7780..8472)
                     /db_xref="GeneID:95692028"
                     /locus_tag="H4W23_RS25825"
                     /old_locus_tag="H4W23_25805"
     CDS             complement(7780..8472)
                     /codon_start=1
                     /db_xref="GeneID:95692028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239820.1"
                     /locus_tag="H4W23_RS25825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_25805"
                     /product="hemolysin III family protein"
                     /protein_id="WP_079037430.1"
                     /transl_table=11
                     /translation="MPEVTPTDAPGSVENPLSLPPKPRLRGWLHAGMFPAVIVAGLVLV
                     AFSDSPRARVACGIYVLTACLLFGISALYHRGNWGPRGEAVLRRLDHANIFLIIAGTYT
                     PLTLLLLPDSTAKPLMWAVWAAALAGIAFRVFWVGAPRWLYTPCYIAMGWAAVFFLPDF
                     MHAGGIAVLVLVIVGGLLYSAGGVIYGMKRPNPSPRWFGFHEVFHSLTLAAFIVHYVGI
                     SLVAYQHA"
     misc_feature    8812..8827
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.27"
                     /tool="antismash"
     misc_feature    complement(9103..9118)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.5"
                     /tool="antismash"
ORIGIN
        1 tgggtgaacg cgcggccggc ccggaagcaa cgttcgggaa cgttataacc ccgacgcggg
       61 gcgatagtaa gcattagatg ctcggaaacg ctaactcccg tatctgcgcg gcaggttcct
      121 cgttgagcgt gttgcgccat tccacgccca gtcgctcgaa tggccctctt atggcccaaa
      181 gatcggttga acgtcacccg ttcgggagag caaccgatga aagcccgatg cgttgttgag
      241 ggagccgggc cgaacagtcc gcgcagtccg agttggatga attcgatcga ggagctcttc
      301 tccatgtccc gtatcgccaa ggccgttgtc ctgaccgtgg gtgccgccgc cgccgtcgcc
      361 ggtgccgccg gtgtcgccgc tgccgactcc atcgccgagg gtgcggccgt gaagtccccg
      421 ggcgtcggct ccggcaacgt cgtccaggtc ccggtccaca tcccggtcaa cgtctgcggc
      481 aacaccgtca gcgtcatcgg cgcgctgaac ccgaccttcg gcaacttctg cgccaacgtc
      541 tgatctcgca cagcggatcg gtccttccga agccggtgcg cccgccccca tcggggtggg
      601 cgcaccggct ttttccttgc ggggactggt gggctactcg ttcggccgca ccgtcgtgcg
      661 ccgttcggtg caacggcgta cgccccttcg aggggcagtc cgggcggggg attcactcga
      721 acggagacgt ggcgctcagt agttccccgg ttgcggaggg ctatacccgc aggcacggtg
      781 ggagggaagt tgtccgacgc atcaggaaaa ggaacacaag tgcgtcccct ggctacacgc
      841 cgaatcaccg ccctggcgat atccgccgcc atcaccctcg gcgcggcggg acccgccgtc
      901 gccggcgaac accatccgtc ctccgcggcc gaccgggccg cccacgcgcc cctccccgaa
      961 gccgccgccc tgctgaccca ggccgaggcg ctcggagacc tcggctccgt gacgaccccc
     1021 gtgaccgaac tcgtcacggc cgccctcaag gccgacgcgg gccagctgcc cgcggccgac
     1081 gccgacgccc tcaagacgaa gatcgcggcg gccgtcgagc aggccaagga aacggccccg
     1141 gcccccctgc cggacacccc cgccccggat ctcccggccc cggatctccc gtccccggat
     1201 ctcccggccc cggatctccc gtccccggat ctcccggccc cggaccttcc ggccccggac
     1261 cttccggccc cggaccttcc ggccccggac cttccggccc cggaccttcc ggccccggat
     1321 ctcccggccc cggatctccc ggctccggac ctccccgctc cggaccttcc cgtgaagccg
     1381 ccggtgagcc tgcccgtcgc cgccggtgcg aaggcggcct ccgccgcgcc cgctgacgtc
     1441 gtggacgacg ccctcgccac ggtcgagaag gccgtcgccg gcctgctcgc cgccgtcacc
     1501 tcgggcgacg ccgccggagt cgtaccccag gtgaccgcga cgctgacctc gctggtcaac
     1561 ctggccgtcg ccaccgtgct cggcagcggt ctgcccgcgc ccgacctggc cggtctgccc
     1621 gccctcccgg cgctcccggt cgccgtgccg gagcttccgg tcgatccgcc ggagctgccc
     1681 gtcgacccgc ccgagctgcc ggtcgacgcg cccgtgaaac cgccggtcgg cctccccgac
     1741 ctgccggtca agcccccgct gcccgtcagg tagtccccgc gaaggcccgt accggtccgg
     1801 cttccgccga ccggtacggg ccttcgcgcg cgacaaatag caccggtcgg catcgatcgg
     1861 gtgcagtgtc gagaaatcct tccctccgga gtttccgggg agcgcacctc tcgttacaga
     1921 aagcaggaaa aggcacagga ttcggtcaag aagcctgtgt ggaacgtgac ttgaaagaac
     1981 agcggaagga ttcccccatg aagtccacca aggtcgccgc gatcgtcgcc ggttcgctcg
     2041 tggcgctggg tgccgccgcg cccgccatgg ccacggaggc gctgaccccc accagcctga
     2101 acggcgggct ggacgcgatc gccgccaacg gcctgaagac ggacatgctg agcagcacca
     2161 ccgacggctc cccggtgaag accgccacgg acaccctggg tcagctcaac gaggccggca
     2221 agggcggttc cgcactgctc ggcggcctgc cgctcggcgg ctgatcgaat gattttctga
     2281 tcggccgccg tgtcgaccct gcacgacgcg gggcaccgct ccctactgtc gggtccatcc
     2341 cacgcgatcg tcgacgtgga ctgaccggtc ggagcgaggg gggatcctcg ccggtctctc
     2401 cctttctcca gaggaagagc ggtatgcggt ccatcatcag caggaaaact ctcaccgcag
     2461 cggcggcggc cggcgtcctg tcgctgaccg gcggattcgc cctcgccgcc aactcccatg
     2521 ccggtacggg tgattccccc ggacctggtt cggcgggcca tgtccagggc cccctccccg
     2581 ccgacgtctg cggcaaggac ggggagctcg gcgagctcat cgcggcagcc gtcggagacc
     2641 tctgcgtcga ggcaggggaa cccggcggct acggcgacga gccgacgcac cctccgacga
     2701 ctcagccgcc gacgacgcat cccccgacga ctcagccgcc gacgaccacc ccgcccgaac
     2761 tccccgacac cggtggtgac tccgcgctca tgggcgcggc cgccatcagc gcggcgctga
     2821 tcatcggcgg caccgtcgtc tacctccggg gcggacggat ctcccgccgt cactgacgcc
     2881 gtcggctgac gagacgtcaa gcgaggccct cgccttcacc ggtgagggcc tcgggcgttg
     2941 ctgcacacgg gggtattccg ccacgtttcc cccgaacgcc tcgttctaca gagcatgact
     3001 ctgcgctctc acgccggcat gggaatcctc atgaccgcgc tcgcctccgc cgccctggcc
     3061 gccccggcct cagccgtcga ggcaccggtg atcgtcccgc tccagggcct ggagccggtg
     3121 ctcccgatgg acgcccccac ggtcgccacc ggtgtgcccg tcccggtgcc cggcgctccc
     3181 accggattcc agaagggcct cggctccctg ccggacctgg ccctgcccag cgtgccgctc
     3241 acgggcaccc tcccgaagac cgtcgtggac gcaccgctgc ccgaggtcct ggacggcagc
     3301 gacccgggcc gggccctcct cagcagcccg cactcggaga tggaggcggc cacccccggc
     3361 gccgtcgtcg gcaacccggt ggaggcccct cgcggcaacg ggctcgccgg actccccgac
     3421 ctggcgaagc ctcaggtcgg tgtcctcacg cccgtgttga gcggagccct cgacccccag
     3481 ctcggtctgg tgccgacctc gcgctgatat cacctcgccc cccgcgcgag tgaagacacg
     3541 attcttcccc gccaccacgg ggatagttga tcgttacaag tgaaggctcg tcgcatggcc
     3601 gcggcccgtg gaccgaggcc cgtcccgtcg tatccggcgg cgggcgcctc cggtccacgg
     3661 gccaaaccct tttcggatct tttcggattt ctttcgtacc gttcggcgca atgaccgtga
     3721 ccagtcttcg tgacgggcgt tgaaggggag tcggccgtga ttcctgcggt cgctttccac
     3781 gtccaaggag ttcttgatgt ctcgcatcgc gaaggctgcc gctgttctcg ccggcacggg
     3841 tgccgtggcc ctcagcggag ccggcctggc cgtcgccgac tcgggcgccg aggccgtcgc
     3901 cgcccactcg cccggtgtcg cctccggcaa cgtcgtccag gtcccggtcc acgtcccggt
     3961 caacctctgc ggcaacaccg tcaacgtcat cggcctgctc aacccggcct tcggcaacac
     4021 ctgcatcaac gccgacggcc acggccacgg ccacgacaac ggtgacggcg gctacggcgg
     4081 ctgatccccc cgttccacca cgtgtgtccc cccggcccgc tcggccgggg ggacacatgc
     4141 gtgtgtgcgg tcaggcgtac cggtagatcc ggctgtggtc gaacagctcc gccggggtga
     4201 ccccccaggg cgccatcggc tggtgccggg cgacgacctt gcggtactgc gcgtccccca
     4261 gcggcggcgg ctccgccggc aggtacgcgg cggaggggtt gcgctgctgc caccgcgacc
     4321 acaccaggtc gacgaaggag tggtgcagcc agaagaccgg gtcgttgacg gaaccgccgc
     4381 cgagcatcag cccgccgacc caacggtgca cccggttgtg gatccggaac cgctcgttgc
     4441 cctggcccga ggcccagccc tccagccggt tgcggaagcc ggaggcgctc gtcgagttcc
     4501 agggcgccac gtcgtacacc cgctcccgca tcacctcttc gagctgggcc tgcgtcggga
     4561 gtgcgacggg gtcgcgtggc cggccgaagt ccctggtcag gaagcgggtc tcggacatcg
     4621 cgtacgtgat gttccaccgc ccggaggagt gggcgaaggg gccggtcatc acctggaggt
     4681 cggcgcggcg gccgttgccg cccatgaagt cctcgcccca gatcgaggag gccgggctgt
     4741 tgtcccgggt ccagtcccag tacgggacgg agaccgtgcc gtcgatccgg cgcagggcct
     4801 tctcgaactc cagcaggaac ctgcggtgcc agggcaggaa gctcggcgcc atgtgggcgg
     4861 tcctgagccg cccctccccg tcggccgtgt agtggtcgat gtgggtgcgg acgaactcgt
     4921 cgtacgcgcc ggtccgcttg agctccagga cggcggcgac gaacttccgg cgctcggcgc
     4981 cggtcaggtc gcgctggttc ttacgcgtgt acaccacgcg ggctccctcc cgtcggttcg
     5041 gtgtggacgc cgtggccggc ggcgaaccgg gacagcggag ccgtccccag ctcgtccacg
     5101 gcggcgcggg tggccgccag cggcgtcggg caggactcgt agtggtcgat cggcgagacg
     5161 taacctccgt cggcgcagcg catcagatgc agcggccggc cgtcgacgag ggcgaccggc
     5221 ctgccgccgg ggccggagaa gccccggatg cggcggcccc cgtacatctc gtcgaaggcg
     5281 agcgggtcgt gcggggcccc ggacggcgct cccggcgggg tgcgggtcgc gatgcggccg
     5341 agcgcgcccc cggtgaaggc ggtgaccgcc agcgcgaaga gggaccgcag tgcgacgcgg
     5401 cgcgggacga tcatgagtga tctcctcggg tcggcaattg ccctctgggc acaacgatga
     5461 aaacccgggt tcgttgcgtc ggaaatgcca gcggcgtgaa tatcgaccgt tcgggtgaaa
     5521 tcggcgcggg ggaagggtgg ttgggccact agcatcccta ttcatgacca cttccaatgt
     5581 cgtttcctcg gcgcgcagcg cctctccgct gggaactctc acggtgattc cgtggtccag
     5641 cgagccttcc gccgacgagg cgggcacgcc gttcctcatg gcgtattcgc tcggcgacgg
     5701 ccgggacgga ccggaggccg gtcagcaggc gatgcggacg gccctggagt cgatgggact
     5761 ctcggtcggc gaccggctct tcgacctgga gaaggacgcc gggatcaacg ccacgctgct
     5821 cgtcgagggc ggctccgccg tgctcaacct gcccttcctg aaggtgcagt gcccggtgcc
     5881 cgacgagtgg caggccgccg cccgccagcg cggccaggtc tacctcatct gctccgtgcg
     5941 cccctggccc gaggcggtcc ccgggcagcc cgtcgacgag gcgcggctgc gcaccttcgt
     6001 ctccgacgag gagatgctcg cggagagcgc ccacgtcctg atgccggtcc ggcgcatcca
     6061 gggctgagcc ggagaacgga agggggtcgc ggcgatggcg acggtgaacg tacagggtgg
     6121 ggtgccgggg cagcggcagg aggagtccgc gccgggggcc gcggggacgg ggctcggcac
     6181 gggccgggcc ttcgcctgga tgctgatggt caccggggcg gcgggagtgc tggccggctg
     6241 ggtgatcacg atcgacaagt tcaagctcct ggaggacccc ggcttccagc ccgggtgcag
     6301 cctgaacccg gtcgtctcct gcggcaacat catgaagagc gagcaggccg cggtcttcgg
     6361 cttccccaac ccgatgctcg ggctcgtcac gtacgccgtc gtcgtcgccg tcggtgccgg
     6421 gctgctctcc ggggcccgct accggggctg gttctggctc ggcctcaacg ccgggatgct
     6481 cttcggggtc ggcttctgca cctggctcat gtaccagtcg ctgtacgaga tcaacgcgct
     6541 ctgcctgtgg tgctgcctgg cctggaccgc gaccatcgtc atgttctggt acgtgaccgc
     6601 gcacaacgtc cgagccgggc tgctgcccgc cccggccggg gtgcggacct tcctcggcga
     6661 gttcgccttc gccctgcccg tcctgcacat cgggatcatc gggatgctga tcctgacccg
     6721 ctggtgggac ttctggacca gctgacgtga accccttcct cctctggccg cgggccgcac
     6781 tgatcgtgct gctcgcggcc gttctcgtgc tcctgctgtc ccacgacggg atcgcgcccg
     6841 gcgggcggca caccgggagc cgggccccgg tggcgccgga gccgctgccc ggggtgccct
     6901 cgggcttctt caccggctcc gacgaggccg gggtgcggcg gatcgcgcag gtgcaggcct
     6961 ggctcggcgg cgagacgctc accgtcgggc acacgtacct gccgggcgac cgctggtcca
     7021 acatcgaggg gcatccggcg ctcttcgagc cctgggcgcg ctggaaggag gagcggccgg
     7081 gacggctctt cgtcctgaac gtgccgctgc tcgaccgcaa cgaggcggga ctgccggaca
     7141 cggaggtgcg cgcggggctg cggcgcggcg cgggcggcgc cttcgacgcg cacttccggg
     7201 tgctcgggga gcggctcgtg gagcacgggc tcgacgacgc ggtgctcgtg ctcggctggg
     7261 agatgaacgg caccacgtac agccatcgct gcggcccgga cccgacggcc tggaaggcgt
     7321 actggcggcg ggtcgtgggg gtgctgcggg acgtggcggg gcagcggttc cgcttcgact
     7381 tcaccccgag ccgggggctc gacgccgtgg cgtggccgct ctgctacccc ggggacgacg
     7441 tcgtcgacat catcgggatg gacgcctacg accagccggc cggggtctcc ttcgaggagc
     7501 aggtgaccga ggagtacggc ctcgagcacc acgtgcggtt cgcggcggcg cacgggaagc
     7561 ccgtctcgta cccggagtgg gggctcttca ggaacgggga caaccccgtc tacgtgcgcg
     7621 ggatgctcga ctggttcgcg gcgcaccgac ccgtctacca gacggtgacg gactactgtc
     7681 cgcacggagt gtgggagtgc gcggacaatc cggagtcggc gcggatcgtc aggggcgcgc
     7741 tgggggctcc cgcccgcccc tgatcgtcgt cgtcgcaggt caggcgtgct ggtaggcgac
     7801 cagcgagatg ccgacgtagt ggacgatgaa ggccgcgagg gtcagggagt ggaagacctc
     7861 gtggaagccg aaccagcggg gtgaggggtt cgggcgcttc atgccgtaga tgaccccgcc
     7921 cgcgctgtag agcagaccgc cgacgatgac gaggacgagg acggcgatgc cgcccgcgtg
     7981 catgaagtcc ggcaggaaga agacggcggc ccagcccatc gcgatgtagc agggcgtgta
     8041 cagccagcgc ggtgcgccga cccagaagac gcggaaggcg atgccggcga gggccgccgc
     8101 ccagaccgcc cacatgagcg gcttcgccgt ggagtcgggg agcaggagca gggtgagcgg
     8161 ggtgtaggtg cccgcgatga tcaggaagat gttggcgtgg tcgaggcggc gcaggacggc
     8221 ctcgccgcgc ggtccccagt tcccccggtg gtagagcgcg ctgatgccga agagcaggca
     8281 tgcggtgagg acgtagatcc cgcaggcgac gcgggcgcgg ggcgagtcgg agaaggcgac
     8341 gaggacgagg cccgcgacga tcacggcggg gaacatgccg gcgtgcagcc agcctctgag
     8401 tcgaggcttc ggcgggagcg acagggggtt ctcgaccgac cctggtgcat cggtcggggt
     8461 cacttccggc atggctgcag tcatgagacc gcatcgtacc tacgcgcccg taggtcccgg
     8521 atatgagtgg cgatgctcac gtgtgcggcc ctctggacat atgggcgcta acatcggatg
     8581 atcaaatgag tgcggtcggc accggatgag cagctacgaa gcatccgggt cgcggccccc
     8641 acggggcata caccaaaaaa acccctcaac aaggagcaat cgtggcgcgc gacaacgcgg
     8701 ctcccaccac cgtcccgacc cagcaccagg agctcgtctc ctgggtgaac gacatcgccg
     8761 agatcaccca gccggacaac atcgtctggt gcgacggatc cgaggcggag tacgagcgtc
     8821 tgtgcgagga gctcgtcgcc aaggggacct tcacgaagct cgacccggtc aagcgtccga
     8881 actcgtacta cgccgcttcc gacccctccg acgtcgcgcg cgtcgaggac cgtacgtaca
     8941 tctgctccga gaaggaggag gacgcgggcc cgacgaacca ctggaaggcc ccggccgaga
     9001 tgaaggagct cttcgcgggc tccgaaggca tcttccgcgg ctcgatgcgt ggccgcacca
     9061 tgtacgtcgt gcccttctgc atgggccccg tcggatcccc gctctccgcc atcggcgtcg
     9121 agatcacgga ctccgcgtac gtcgccgtct ccatgcgcac catgacccgc atgggacagc
     9181 ccgtcctgga cgagctcggc accgacggct tcttcgtcaa ggccgtccac accctcggcg
     9241 ccccgctgga ggagggccag gaggacgtgc cgtggccctg caacaccacc aagtacatct
     9301 cgcacttccc ggagacccgc gagatctggt cgtacggctc gggctacggc ggcaacgccc
     9361 tgctcggcaa gaagtgctac gcgctccgca tcgcctccgt catggcgcgt gacgagggct
     9421 ggctcgccga gcacatgctc atcctcaagc tgaccccgcc gcagggcgag tcgaagtacg
     9481 tggccgcggc cttcccgtcg gcctgtggca agaccaacct ggccatgctg gagcccacga
     9541 tctccggctg gaccgtcgag accatcggcg acgacatcgc ctggatgcgc ttcggcgagg
     9601 acggtcgcct ctacgcgatc aaccccgagg ccggtttctt cggcgtcgcg cccggcaccg
     9661 gcgagcacac caacgccaac gccatgaaga ccatgtgggg caactccgtc ttcacgaacg
     9721 tcgccctcac ggacgacggc gacgtgtggt gggaggggat gaccgagacg gcccccgcgc
     9781 acctcacgga ctggaagggc aacgactgga cgcccgcgtc cgagacgccg gccgcccacc
     9841 cgaacgcccg cttcaccgtc ccggccgggc agtgcccgat catcgcgccg gagtgggagg
     9901 acccgaaggg cgtgccgatc tcggcgatcc tcttcggtgg ccgccgcgcc tccgccgtcc
     9961 cgctggtcac cgagtccttc gactggaacc acggcgtctt cctcggtgcg aacgtcgcct
    10021 ccgagaagac cgccgccgcc gagggcaagg tcggcgagct gcgccgcgac cccttcgcca
    10081 tgctgccgtt ctgcggctac aacatgggcg actacatggc ccactgggtc aaggtcggcg
    10141 ccaacgccga tgcggcgaag ctgccgaaga tctactacgt gaactggttc cgcaagaacg
    10201 acgcgggcaa gttcgtgtgg cccggcttcg gcgagaacag ccgcgtcctg aagtggatcg
    10261 tggagcgcct ggagggcaag gccgagggcg tcgagacccc gatcggcatc ctgccgaccc
    10321 cggagtcgct cgacaccgag ggcctggacc tgccggccgc ggacctggag ttcctcctca
    10381 aggtcgaccg tgacgtgtgg cgcgaagagg ccgc
//
