LOCUS       NZ_CP059991            10816 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptomyces gardneri strain ATCC 15439 chromosome, complete genome.
ACCESSION   NZ_CP059991
VERSION     NZ_CP059991.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces gardneri
  ORGANISM  Streptomyces gardneri
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9054805)
  AUTHORS   Kim,W., Hwang,S. and Lee,N.
  TITLE     Genome-scale analysis on the transcription unit architecture of
            Streptomyces venezuelae ATCC 15439
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9054805)
  AUTHORS   Kim,W., Hwang,S. and Lee,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2020) Biological Sciences, KAIST, 291 Daehak-ro,
            Yuseong-gu, Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 6.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP013129
            Genome Coverage                   :: 187.8x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015710995.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 17:12:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,199
            CDSs (total)                      :: 8,101
            Genes (coding)                    :: 7,918
            CDSs (with protein)               :: 7,918
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 70 of 184
            Pseudo Genes (incomplete)         :: 147 of 184
            Pseudo Genes (internal stop)      :: 16 of 184
            Pseudo Genes (multiple problems)  :: 48 of 184
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:23:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1027115
            Orig. end                         :: 1037931
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059991.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10816
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10816
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10816
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5816](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5816)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(351..2048)
                     /db_xref="GeneID:95687848"
                     /locus_tag="H4W23_RS04720"
                     /old_locus_tag="H4W23_04710"
     CDS             complement(351..2048)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95687848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038128.1"
                     /locus_tag="H4W23_RS04720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_04710"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_055644997.1"
                     /transl_table=11
                     /translation="MRRRSVSLLGLAGLLVLPLTSIPQAQAQAPAQPRAQERGEAAAAA
                     DSLVSAGKPVTASSVEGTGFEAGRAVDGNTATRWASLEGVDPQWVRIDLGATHTVSRVK
                     LNWEAAYGKAYRIQTSADGTNWTDVYATTTGDGGVDDLTVSGSGRYLRMHGTSRGTPYG
                     YSLWEFEVYGAPSGGGVPGGPAVPFGSHLKPYAAGTLKPSGAQAVLDQKVVEYYNKWKA
                     AFVRQNCGNGWYQIISPDADHPYVAEAQGYGMVVTATMAGADPDAKKIFDGLVKWKIDH
                     PSSVNPNLLAAEQDVACKSVNGGDGATDGDMDVAYGLLLADKQWGSAGTYNYKDLALKH
                     IAAIKKDELNPTTKLLKLGDWSSSGDQYYFISRTSDWMVDHFRAFRTASGDTTWDAVRS
                     AHQTQISRLQSTYASGTGLLPDFVVDTNTTPKPAPGQVLEDPNDGAYWWNACRTPWRIA
                     DDAVTSGDATSLAAARKLNSWIKTKTGGDPNKIAIGYKLNGTQISAGSEAAFFAPFAVA
                     AMTDPGSQAWLDALWNKMLTTPVDTSSYFSASIQLQVMITASGNHWVP"
     gene            complement(2164..3378)
                     /db_xref="GeneID:95687849"
                     /locus_tag="H4W23_RS04725"
                     /old_locus_tag="H4W23_04715"
     CDS             complement(2164..3378)
                     /EC_number="3.4.-.-"
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95687849"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019066219.1"
                     /locus_tag="H4W23_RS04725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_04715"
                     /product="S8 family peptidase"
                     /protein_id="WP_055644996.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 4.3e-35, bitscore:
                     120.3, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALPQHTRARLALTVSAATAAALLGTFAAVPAQAAPAEGVVLAAG
                     SADAVPGSYIVTLKQGAGFKAASAKGRNLISGYGGTVRKTFGSAINGYTATLDATEARR
                     LAADPAVASVEQNQVFRADATQTNAPWGLDRIDQANLPLNGTYTYPDSAGAGVTAYVID
                     TGVRISHAQFGGRAVNGYDAVDGDTVAQDGNGHGTHVATTIAGSTYGVAKAAKIVAVRV
                     LDNAGSGTTAGVIAGIDWVTSHHAAGAPAVANLSLGGGASTALDNAVKNSIADGVTYAV
                     AAGNSGVNASSSSPARVAAAITVGATSNTDAKASWSNYGSVLDLFAPGVSITAGWHTSD
                     TATNTISGTSMATPHVAGAAAVYLAGHASATPAQVATALVNGSTPNKVTSPGSGSPNRL
                     LRLVP"
     gene            3797..4270
                     /db_xref="GeneID:95687850"
                     /locus_tag="H4W23_RS04730"
                     /old_locus_tag="H4W23_04720"
     CDS             3797..4270
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95687850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007497499.1"
                     /locus_tag="H4W23_RS04730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_04720"
                     /product="GreA/GreB family elongation factor"
                     /protein_id="WP_055644995.1"
                     /transl_table=11
                     /translation="MTGGPEPLSAVARAAFERELADLRTERETVAVTLREGGGDQVGDR
                     ADQADELQRVTELQRLDARIAEIEGRLREGAVAGPPSTDEVGVGSSVTVRFADATETTV
                     QIGEVAEVLDATMVTVDSPLGRALLGHRAGDTVTYDTPEGPTTAVVLSLGDAS"
     gene            complement(4362..4997)
                     /db_xref="GeneID:95687851"
                     /locus_tag="H4W23_RS04735"
                     /old_locus_tag="H4W23_04725"
     CDS             complement(4362..4997)
                     /codon_start=1
                     /db_xref="GeneID:95687851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763512.1"
                     /locus_tag="H4W23_RS04735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_04725"
                     /product="DUF1345 domain-containing protein"
                     /protein_id="WP_055644994.1"
                     /transl_table=11
                     /translation="MHRRPVDAVPRLVAAAVVGAATGALVGLLWDTPLGILAGIATAET
                     LFVVAGWLVLWPMDAAATHRNVRREDFRPVLEELVVVGTAVCGLVGIIVMLMVGHSDLG
                     HAAAATALCGVFMAWAALHLMYAARYAYDYYALHPGGGIDFNTHDPPRYSDFLYFSFNL
                     GMTYQVSDTNVSTSSLRAIVLRHCLLSYVFGASILATTINLVTGIVTG"
     gene            complement(5001..5816)
                     /db_xref="GeneID:95687852"
                     /locus_tag="H4W23_RS04740"
                     /old_locus_tag="H4W23_04730"
     CDS             complement(5001..5816)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95687852"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240918.1"
                     /locus_tag="H4W23_RS04740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_04730"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_055644993.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1.6e-87, bitscore:
                     291.7, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPDVTNNLHRELAPITPAAWAEIEEEARRTFRRHVAGRRVVDV
                     PDPAGPGLAAVGVGHLAEIEPPAPGVSARLREARPIVELRVPFTVSRAAVDDVERGSKD
                     SDWQPVKDAARTMAYVEDRTIFDGYGAAGVEGLRFRSSNPVLRLPAEPREYPDAVSRAL
                     TELRLVGVDGPYALLLGAEEYRAVSETSDHGYPIAAHLGRMLDGSPIWAPALSGGFVLS
                     TRGGDFELLLGQDVAIGYAGHDAHGIDLYFHETLTFLAHTDEAVVVLEP"
     gene            complement(5813..6808)
                     /db_xref="GeneID:95687853"
                     /locus_tag="H4W23_RS04745"
                     /old_locus_tag="H4W23_04735"
     CDS             complement(5813..6808)
                     /codon_start=1
                     /db_xref="GeneID:95687853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240919.1"
                     /locus_tag="H4W23_RS04745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_04735"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_055644992.1"
                     /transl_table=11
                     /translation="MVVAQPVLTPPSQAAVFLVATVAPGGETVVRDALRDLSGLTRSVA
                     FRSPESGLRCVAGMGSEAWDRLVGDPRPRDLHPFPALRGPRHRAPATPGDLLFHLRARR
                     MDLCFELARLILDRLGDAVTVVDEVHGFTYFDERDLLGFVDGSENPSGTAAAEAVHIGD
                     EDPDFRGGSYVVVQKYVHDMTAWDSLTVEEQEHVIGRTKQANVELPDGVKPSDSHVALT
                     TVVDDDGTERQILRQNMPFGRVGSGELGTYFIGYARTPDVTERMLRNMFLGDPPGNTDR
                     ILDFSRAVTGCLFFVPSADLLDDLPAAPSAAATSPGDSLGIGSLKGAPAS"
     gene            7094..9364
                     /db_xref="GeneID:95687854"
                     /locus_tag="H4W23_RS04750"
                     /old_locus_tag="H4W23_04740"
     CDS             7094..9364
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95687854"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1134: flavodoxin"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018843023.1"
                     /locus_tag="H4W23_RS04750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_04740"
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="WP_055644991.1"
                     /transl_table=11
                     /translation="MGPTPQGDPEFHPYHHPAAGWGAAKSVTRFLVAERALVDGPRAIM
                     KMNHENTGFDCPGCAWPDDTKGLHLDICENGIKHVTWEMTSKRVGREFFAAHSVTELAG
                     WSDYDLENQGRLTEPMVYDPDTDHYVPIGWKDAFETVGRTLRELDSPHRAAFYTSGRLG
                     NEATFLYQLMARELGTNNLPDCSNMCHEASGRALQASLGTGKGTVDLKDWEAADALFIL
                     GVNAASNAPRMLTALAEAHHRGAQIVHVNPLVEAAATRTIVPHDFLDMALFKTTRTSTL
                     NLQPRIGGDMALLRGMAKAILEQSETDPKALDREFIERHTSGFDEYRAVCEAASWEQLE
                     KQSGLSRAEILAAARVYGEADRSIVSWCLGVTQHEHGVDTVREIVNLLLLRGNLGREGA
                     GPSPVRGHSNVQGNRTCGIDHRPSDAFLDRLAEACGIEPPREHGLDTVRTITAMHEGDV
                     TVFVGMGGNFALAAPDTPYTYAALANCDLTVQVSTKLNRSHIVHGRRALILPCLGRTEK
                     DHQRKGIQSTSVEDSMSMVHLSVGMKRPASPHLLSEPAIVAGMARAALPGSATPWEWYV
                     EDYDRIRDTMARALDGFEDFNRRVRLPLGFRIKQPARELVFLTPSGRAEFSAAALPDVV
                     PAPGTLALGTMRSHDQWNTTIYSDNDRYRGIRNLRTLVFMNRADMRERGIPDLGSVDIT
                     STAKDGSRRFLRGYVAVPYDIPRGCAAGYMPEMNVLCALGDYSTQSDQPIMKHVKVTIG
                     PAA"
ORIGIN
        1 gcatccgccg gcaggaccgg atcaactggc agatccggtt cgtcgagggc gacctcaccc
       61 cggacgagcg cgagcgcctg tccggggccc tgtccgcgcc cgcccccgac ctcaccgacg
      121 agcagcgcgc cgggtggctc gccgagctga ccggggtggc gttcgcctcg gacggtgcgc
      181 tgcccttccg ggacaacgtc gaccacgcgc gccggcacgg cgtcaccgcg atcgccgaac
      241 ccggcgggtc gatccgctcg accgatgtcg aggacgcctg ccgcgcgcac gggatcgctc
      301 tcgcgcgcac cggactgagg ctcttccacc actgatcggc gagccgcgct ttacgggacc
      361 cagtggttgc ccgaggccgt gatcatgacc tggagctgga tgctggccga gaagtagctg
      421 ctcgtgtcga ccggggtggt cagcatcttg ttccagaggg cgtccagcca ggcctggctg
      481 cccgggtcgg tcatggccgc cacggcgaac ggcgcgaaga acgcggcctc gctgcccgcc
      541 gatatctggg tgccgttgag cttgtagccg atggcgatct tgttcgggtc gccaccggtc
      601 ttcgtcttga tccagctgtt gagcttgcgc gccgccgcga gcgaggtggc gtcgccgctg
      661 gtcaccgcgt cgtccgcgat acgccacggg gtacgacacg cgttccacca gtaggcgccg
      721 tcgttcgggt cctccaggac ctggccgggg gcgggcttgg gcgtggtgtt cgtgtcgacg
      781 acgaagtcgg gcagcaggcc ggtgccggag gcgtacgtcg actggagccg cgagatctgg
      841 gtctggtgcg cgctgcgcac ggcgtcccag gtcgtgtcgc cggaggccgt acggaaggcg
      901 cggaagtggt cgaccatcca gtccgaggtg cgggagatga agtagtactg gtcgccggag
      961 ctgctccagt cgcccagctt gaggagcttg gtggtcgggt tgagctcgtc cttcttgatc
     1021 gcggcgatgt gcttgagcgc gaggtccttg tagttgtagg tgccggcgct gccccactgc
     1081 ttgtcggcga ggagcaggcc gtaggccacg tccatgtcgc cgtcggtggc gccgtcaccg
     1141 ccgttgacgc tcttgcaggc cacgtcctgt tcggccgcga ggaggttcgg gttgaccgag
     1201 gacgggtggt cgatcttcca cttcacgaga ccgtcgaaga tcttcttcgc gtcggggtcg
     1261 gcgcctgcca tcgtcgcggt gacgaccatg ccgtacccct gcgcctcggc gacgtacggg
     1321 tggtcggcgt ccggcgagat gatctggtac cagccgttgc cgcagttctg ccggacgaag
     1381 gcggccttcc acttgttgta gtactcgacg accttctgat cgagcacggc ctgggcgccg
     1441 gagggcttca gggtccccgc cgcgtacggc ttgaggtggc tgccgaaggg gacggccggc
     1501 ccgccgggga cgccgccgcc gctcggggcg ccgtagacct cgaactccca gagggagtag
     1561 ccgtacgggg tgccgcgcga ggtcccgtgc atgcggaggt accggccgct gccggagacc
     1621 gtgaggtcgt cgacgcctcc gtcgccggtc gtggtcgcgt agacgtcggt ccagttcgtg
     1681 ccgtcggccg aggtctggat ccggtacgcc ttgccgtagg cggcctccca gttgagtttc
     1741 acccgggaga cggtgtgcgt cgcgccgagg tcgatcctca cccactgggg gtcgacgccc
     1801 tccagactgg cccagcgcgt ggcggtgttg ccgtccaccg cccggccggc ctcgaagccc
     1861 gtgccctcga cggacgaggc cgtgaccggc tttccggcgg agaccaggct gtcggcggcg
     1921 gccgccgcct ctccccgctc ctgtgcgcgc ggctgcgccg gcgcctgtgc ctgtgcctgc
     1981 gggatcgacg tcaggggcag caccagcagt ccggcgagcc cgagaagcga gacgcttcga
     2041 cgacgcatga caactccctt tccttcgcgg ctcattcgtt aggaagtttt cctaaccatt
     2101 gagccgcgag ggtagagagg gtcgggggtc cgcgcaagac ccccgacacc ggtcgtgtgc
     2161 gtcctacggg acgagccgca gcaggcggtt gggcgaaccg ctgcccgggc tggtcacctt
     2221 gttcggggtc gagccgttca ccagagcggt cgcgacctgc gccggagtgg cggaggcgtg
     2281 tcccgcgagg tagacggcgg cggctcccgc gacgtgcggg gtggccatcg aggtgcccga
     2341 gatcgtgttg gtggccgtgt cgctcgtgtg ccagcccgcg gtgatcgaca cgccgggggc
     2401 gaagaggtcg agcaccgaac cgtagttgga ccagctcgcc ttggcgtccg tgttgctcgt
     2461 cgcgccgacc gtgatggcgg cggcgacccg ggcgggcgac gaggaggacg cgttgacgcc
     2521 ggagttgccc gcggcgacgg cgtaggtgac gccgtccgcg atggagttct tcaccgcgtt
     2581 gtcgagggcg gtggaggcgc cgcccccgag ggagaggttg gcgacggccg gggcccccgc
     2641 cgcgtggtgg gaggtgaccc agtcgatgcc cgcgatgaca ccggcggtgg tgccggagcc
     2701 cgcgttgtcg aggacccgga cggcgacgat cttcgcggcc ttggccacgc cgtacgtcga
     2761 tcccgcgatg gtggtggcga cgtgcgtgcc gtggccgttg ccgtcctggg cgacggtgtc
     2821 gccgtcgacc gcgtcgtagc cgttcacggc ccgcccgccg aactgcgcgt gcgagatgcg
     2881 tacgccggtg tcgatgacgt acgcggtgac gccggcgccc gccgagtcgg ggtacgtgta
     2941 cgtgccgttc agcggcaggt tcgcctggtc gatgcggtcc aggccccaag gggcgttggt
     3001 ctgcgtggcg tcggcgcgga agacctggtt ctgctcgacg gaggcgacgg cggggtcggc
     3061 ggcgaggcgg cgggcctccg tcgcgtcgag ggtggccgtg tagccgttga tggcggagcc
     3121 gaaggtcttg cggacggtgc cgccgtaacc ggatatcagg ttccgaccct tggcggaggc
     3181 ggccttgaag cccgcgccct gcttcagggt gacgatgtag ctgccgggca ccgcgtcggc
     3241 cgatccggcc gcgaggacga caccttcggc gggggccgcc tgggcgggga cggcggcgaa
     3301 ggtgccgagc agggcggccg cggtcgcggc ggagaccgtc agcgcgagcc gggccctggt
     3361 gtgctgaggg agtgccatac gagggagtcc tcctcttcgg cgtcgcgcct cttgtggggc
     3421 ggcgcgacag tggggggcgc gtgtgggtca cacgcacggc cgggagccgt gtggggcgct
     3481 ccgggtgatc agcagactgt ccggtctacg ggcgtagctc aagggagttg ggcgcttgtc
     3541 atgcatctgc cacactctcg caatcgaact cccgtcgaac ccgtggataa cgcccacggg
     3601 cccaccggga ggacgccacc gaaccactcc gggacgcgca ccctgagcca cctcgcggcg
     3661 cacgccgaac caccccggga cgcacctcga accacctgac gaccctggac gaaatgcccc
     3721 gattcgcctg ataccttcgg cttgccgtga ccgatccgcg gcacacatcc gtgccgcccg
     3781 cccgaaggga tcagacatga ccggtggacc cgagcccctc agcgccgtcg cccgagcggc
     3841 gttcgagcgc gaactcgccg acctgcgcac cgagcgcgag acggtcgccg tgaccttgcg
     3901 cgagggcggc ggcgaccagg tgggcgaccg cgccgaccag gccgacgagc tgcagcgcgt
     3961 cacggagctc cagcgtctgg acgcccgtat cgccgagatc gaggggcggc tgcgcgaggg
     4021 cgccgtcgcc gggccgccga gcaccgacga ggtcggcgtc ggcagctccg tgaccgtgcg
     4081 gttcgcggac gccacggaga cgaccgtcca gatcggcgag gtcgccgagg tcctggacgc
     4141 gaccatggtc accgtcgaca gcccgctcgg ccgcgcgctg ctcggtcatc gcgccggcga
     4201 caccgtcacg tacgacacgc ccgagggccc gacgaccgcg gtggtgctgt cgctcggcga
     4261 cgcgtcatag ccccacgtcc cgagccgtcc cggccgaccg agcaggtcct acaggcccta
     4321 caggccgagc tggcacgccc gaccgaacgg gcgccgcccg ctcagcccgt gacgatgccg
     4381 gtcaccagat tgatggtggt ggccaggatg ctggcgccga agacgtacga cagcagacag
     4441 tgccggagga cgatcgcgcg gagggacgac gtcgagacgt tcgtgtcgga gacctggtag
     4501 gtcatgccga ggttgaagct gaagtacagg aagtcgctgt accgcggcgg gtcgtgggtg
     4561 ttgaagtcga tcccgccgcc gggatgcagg gcgtagtagt cgtacgcgta gcgggcggcg
     4621 tacatcaggt ggagcgccgc ccaggccatg aagacgccgc agagggccgt cgcggcggcg
     4681 gcgtggccca ggtccgagtg gccgaccatg agcatcacga tgatgccgac caggccgcac
     4741 acggcggtcc ccacgacgac gagttcctcg aggacgggcc ggaaatcctc ccgccggacg
     4801 ttgcgatggg tggcggccgc gtccatgggc cagagcacga gccaccccgc gacgacgaag
     4861 agggtctccg ccgtggcgat gccggcgagg atgcccagcg gcgtgtccca gagcaggccg
     4921 acgagcgcgc cggtcgccgc cccgacgacc gccgctgcga cgaggcgcgg gacggcgtcg
     4981 accggcctgc ggtgcacggg tcagggctcc aggacgacca cggcttcgtc cgtgtgggcg
     5041 aggaaggtca gcgtctcgtg gaagtacagg tcgatgccgt gcgcgtcgtg acccgcgtag
     5101 ccgatcgcca cgtcctggcc gagcagcagc tcgaagtcgc cgccccgggt ggagaggacg
     5161 aagccgccgc tcagggccgg tgcccagatc ggggagccgt cgagcatccg ccccaggtgg
     5221 gcggcgatcg ggtagccgtg gtcggaggtc tcgctgaccg cccggtactc ctcggcgccg
     5281 agcagcagcg cgtagggccc gtcgacgccg acgagtcgca gctccgtcag ggcgcggctg
     5341 accgcgtcgg ggtactcgcg cggctcggcg ggcagccgca gcaccgggtt ggaggaccgg
     5401 aagcgcagcc cttcgacacc ggcggcaccg tacccgtcga agatcgtccg gtcctcgacg
     5461 tacgccatcg tgcgcgccgc gtccttgacg ggctgccagt cggagtcctt ggagccgcgc
     5521 tcgacgtcgt ccacggcggc ccgggagacg gtgaacggca cgcgcagttc cacgatcggc
     5581 cgtgcctcgc gcagccgcgc cgagacaccg ggagcgggcg gctcgatctc ggcgaggtgg
     5641 ccgacgccga cggctgcgag cccggggccc gccgggtcgg gcacgtcgac gacgcggcgt
     5701 cctgcgacat ggcgccggaa ggtgcggcgc gcctcctcct cgatctccgc ccaggccgcg
     5761 ggggtgatcg gggcgagttc ccggtgcagg ttgttcgtca cgtcgggagt cgtcatgacg
     5821 cgggggctcc tttcagactg ccgatgccca gggagtcccc cgggctcgtg gcggccgcgg
     5881 acggggcggc ggggaggtcg tcgagcaggt cggcgctcgg cacgaagaag aggcagccgg
     5941 tgacggcgcg ggagaagtcg aggatgcggt ccgtgttgcc cggggggtcc ccgaggaaca
     6001 tgttgcgcag catccgctcg gtgacgtcgg gcgtgcgggc gtagccgatg aagtacgtgc
     6061 ccagctcgcc actgccgacc cgcccgaacg gcatgttctg gcggaggatc tgccgctcgg
     6121 tgccgtcgtc gtcgacgacc gtggtgagcg cgacgtggga gtcggacggc ttgacgccgt
     6181 cgggcagttc gacgttggcc tgcttggtgc ggccgatgac gtgctcctgc tcctcgacgg
     6241 tgagggagtc ccaggccgtc atgtcgtgca cgtacttctg cacgacgaca tagctcccgc
     6301 cccggaagtc cgggtcctcg tcgccgatgt gcaccgcctc ggcggcggcc gtgcccgagg
     6361 gattctcgct gccgtcgacg aagccgagca gatcgcgctc gtcgaagtag gtgaagccgt
     6421 ggacctcgtc gacgaccgtc accgcgtcgc ccagccggtc caggatcagc cgggccagtt
     6481 cgaagcagag gtccatgcgc cgggcacgta gatggaacag caggtcgccc ggggtcgccg
     6541 gcgcgcggtg gcgggggccg cgcaaggccg ggaaggggtg caggtcgcgc gggcgcgggt
     6601 cgccgacgag ccggtcccag gcctccgagc ccataccggc gacgcagcgg agccccgatt
     6661 cgggggaccg gaacgccacc gaacgggtca ggcccgacag gtcccggagg gcgtcccgta
     6721 cgaccgtctc cccaccgggg gccacggtgg ccaccaggaa gacggccgcc tgggaggggg
     6781 gtgtgaggac gggctgcgcg acgaccatgg aactcccgcc gaaagggatg aacaggggca
     6841 taccgaagca gtggggcggc gccagcgtat ccgcgcgcgg aggggtccgc cctgccggac
     6901 ggggcgcacg ggaggggccc cggaggtcga gtggcaggcc ccccgcaccg gacctagcct
     6961 gcggaggaga gccgtgtcgc ctccccgtgc ccgcgggccg gaagggtgga gagtcgtgaa
     7021 tgcccacaac gacgaccagg aacgcccgga gcgccaggaa caccctgaac gaccgcagca
     7081 cccggaagcc cgcgtggggc ccactcccca gggcgacccg gagttccacc cgtaccacca
     7141 ccccgcggca ggctggggcg cggcgaagag cgtgacgcgt ttcctcgtcg ccgagcgcgc
     7201 gctggtggac ggaccccggg cgatcatgaa gatgaaccac gagaacaccg ggttcgactg
     7261 ccccggctgc gcgtggccgg acgacaccaa gggcctccac ctcgacatct gcgagaacgg
     7321 catcaagcac gtcacctggg agatgacgag caagcgggtc gggcgcgagt tcttcgccgc
     7381 ccattcggtg accgagctcg ccggctggag cgactacgac ctggagaacc agggtcggct
     7441 gaccgagccg atggtctacg acccggacac ggaccactac gtaccgatcg gctggaagga
     7501 cgccttcgag acggtcggcc gcaccctgcg cgagctggac agcccgcacc gggcggcctt
     7561 ctacacctcc ggccggctcg gcaacgaggc cacgttcctg taccagctga tggcccgcga
     7621 gctcggcacg aacaacctgc cggactgctc caacatgtgc cacgaggcga gcggccgcgc
     7681 cctccaggct tcgctgggca cgggcaaggg gaccgtcgac ctcaaggact gggaggctgc
     7741 cgacgcgctg ttcatcctcg gggtcaacgc cgcctccaac gcgccccgga tgctcaccgc
     7801 cctcgccgag gcgcaccacc gcggcgcgca gatcgtgcac gtcaatccgc tggtcgaggc
     7861 ggccgccacc cgcacgatcg tcccccacga cttcctcgac atggccctgt tcaagacgac
     7921 gaggaccagc acgctgaacc ttcagccgcg catcggcggc gacatggcgc tgctgcgcgg
     7981 catggccaag gcgatcctgg agcagtcgga gaccgacccc aaggccctgg accgggagtt
     8041 catcgagcgt cacacgtccg gcttcgacga gtaccgcgcg gtgtgcgagg ccgcgtcgtg
     8101 ggagcagctg gagaagcagt ccgggctgag ccgcgccgag atcctcgcgg cggcacgggt
     8161 gtacggggag gccgaccgct cgatcgtcag ctggtgtctg ggcgtcaccc agcacgagca
     8221 cggcgtcgac accgtccggg agatcgtcaa cctccttctg ctgcgcggca atctggggcg
     8281 cgagggcgcg ggcccctccc ccgtccgcgg tcacagcaac gtccagggca accgcacctg
     8341 cggcatcgac caccgcccct ccgacgcgtt cctcgaccgt ctcgccgagg cctgcgggat
     8401 cgagccgccc cgcgagcacg gtctggacac cgtccggacg atcacggcga tgcacgaggg
     8461 cgacgtcacg gtgttcgtcg gcatgggcgg caacttcgcc ctcgcggctc cggacacccc
     8521 gtacacctac gccgcactgg ccaactgcga cctcaccgtg caggtgagca ccaagctcaa
     8581 ccgcagccac atcgtccacg gccgccgggc gctcatcctg ccgtgcctgg gccggaccga
     8641 gaaggaccat cagcgcaagg ggatccagag cacctccgtc gaggactcga tgagcatggt
     8701 ccacctgtcc gtgggcatga agcgccccgc ctcgccgcac ctgctctccg agccggccat
     8761 cgtggcgggc atggcgcgcg cggcgctgcc cgggagcgcg accccctggg agtggtacgt
     8821 cgaggactac gaccgcatcc gcgacacgat ggcccgcgcc ctcgacggct tcgaggactt
     8881 caaccggcgg gtccggctgc ccctcggctt ccgtatcaag cagcccgccc gggaactggt
     8941 cttcctcacg ccgtccgggc gcgccgagtt ctccgcggcc gcactgcccg acgtcgtccc
     9001 cgcgcccggc accctggcgc tgggcaccat gcggtcccac gaccagtgga acaccacgat
     9061 ctactccgac aacgaccgct accggggcat caggaacctc cgcacgctgg tcttcatgaa
     9121 ccgggccgac atgcgcgagc gcggcatccc cgacctcggg tccgtcgaca tcacaagcac
     9181 cgcgaaggac ggcagcaggc ggttcctccg cggctacgtc gccgtcccgt acgacatccc
     9241 ccggggctgc gccgccggct acatgccgga gatgaacgtg ctgtgcgcgc tcggcgacta
     9301 cagcacccag agcgaccagc cgatcatgaa gcacgtcaag gtcacgatcg gtccggcggc
     9361 ctgacgagcc gttcgaggag cgcgtacgcg cgctgctccg cggcggccag ttcgacgggg
     9421 tccatctccg cgggccacag ttcgcccgcg gcggcgtcgt cggcctcgcg cagctcgacc
     9481 acggccgcgg cgaggcgcgt ctgcaccagc ggggacgacg cctgagcccc cgcggccagg
     9541 gcgtgttccg cggcgtcggc ggcgtccgtg cacgcggcca gcgcgcgctg cgcccggacc
     9601 gccgcccggt cgtgcaccac gagcagcgcg cagaccaggg cgatgcccat cccgaggacg
     9661 ctggccagcg cccggtcccg gatgagcacg tcggtcgggg agtcggcggc caggtcggtc
     9721 atcagcaggg cgagcggggt caggaagacg acgccgagag cgtagttgcg ggccaccacg
     9781 tattcgagca ggaactccag gagcacgatc accaggacca gcaccacggg ttcggggcgc
     9841 agggccagca cgccgagggc gagggccagc ccgacgaccg tgccgagggt gcgctggacg
     9901 gcgcgctggg tcgccgtgcg gacattgacc gagtgcagga cggcggcggc ggacacggcc
     9961 gcccagtagc cgtggccgag gccgagcagg agggccgcgc cgcccgcgag accggtgccg
    10021 aggaccatcc gcagggcggg gacgagcagg acggaggcgt gcgcggagcg gctgcccgtc
    10081 agcagtcggg cggcgcggag tccggcggcg cgggaggtgg tggggtcggg cgactcccgg
    10141 ccggggacga tgccgtcccg cgccggcacg gatggacggc gggcgggttc ggacggaccg
    10201 gcgggttcgg acggaccggc gacgtcggac aggcgggcgg cgtctgacag gcgggcgagt
    10261 tcgggcagga tccgcagcgc ccgccgacga cggctcacga gcaggcggac ctgggcacgg
    10321 agggcgcgga cctggccggt ggggtcgtcg ggcgggcggc gggcgcagcc gatgagcagc
    10381 gaccaggcga ggtcggcgag gcgcaggcag gtgccgtccc ggtcgccgcg cccggcatcg
    10441 gcggccgtcg ggggcacccg gccgagggtg cggtacgcct ggaggacggc ggcggtcgcg
    10501 cggtggcgcc ggagcccgct cccgtacgcc gccgctcctg aggcctcccg gcggttcggg
    10561 agctccagga ggtccgcgag ctcgcggagc gccgcggcca cggcgaggcg ctccggccgg
    10621 tccgggtgga cgaaccggcc ggacagggcg aggacccagg ccacggccgc gcccgccccg
    10681 gccaggaggg tgtggggcag gacgtcggcc gtggtcgagg agccgttggc cgccacggcg
    10741 aaggagaaga gcagcaggac cgctccgagg ccgctcaggc gggccgcgtc gcaggcgagc
    10801 ttggcgatcc cggcca
//
