LOCUS       NZ_CP059991            10420 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptomyces gardneri strain ATCC 15439 chromosome, complete genome.
ACCESSION   NZ_CP059991
VERSION     NZ_CP059991.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces gardneri
  ORGANISM  Streptomyces gardneri
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9054805)
  AUTHORS   Kim,W., Hwang,S. and Lee,N.
  TITLE     Genome-scale analysis on the transcription unit architecture of
            Streptomyces venezuelae ATCC 15439
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9054805)
  AUTHORS   Kim,W., Hwang,S. and Lee,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2020) Biological Sciences, KAIST, 291 Daehak-ro,
            Yuseong-gu, Daejeon 34141, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 6.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP013129
            Genome Coverage                   :: 187.8x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015710995.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 17:12:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,199
            CDSs (total)                      :: 8,101
            Genes (coding)                    :: 7,918
            CDSs (with protein)               :: 7,918
            Genes (RNA)                       :: 98
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 70 of 184
            Pseudo Genes (incomplete)         :: 147 of 184
            Pseudo Genes (internal stop)      :: 16 of 184
            Pseudo Genes (multiple problems)  :: 48 of 184
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:23:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 638776
            Orig. end                         :: 649196
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP059991.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10420
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="4"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10420
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10420
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5420](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5420
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    116..129
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.01"
                     /tool="antismash"
     gene            1258..1722
                     /db_xref="GeneID:95687524"
                     /locus_tag="H4W23_RS03075"
                     /old_locus_tag="H4W23_03065"
     CDS             1258..1722
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95687524"
                     /gene_functions="regulatory (smcogs) SMCOG1120: AsnC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031431.1"
                     /locus_tag="H4W23_RS03075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_03065"
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="WP_024757018.1"
                     /transl_table=11
                     /translation="MDRIDLHILRELQNDGRLSNQELAQRVGLSPSPCLRRVRQLEQDG
                     VIQGYRAIIDPEAVGRGFEVLVSVEVRRDRETVEAFEEALQDVPDVIEAYRLFGSPGCL
                     LRIAVADLAAYERLWIERLTTLAGVTEVNSQIIMKRIKEPKGMPVDVRGA"
     misc_feature    1259..1272
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    1745..1759
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.45"
                     /tool="antismash"
     misc_feature    complement(1832..1847)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.49"
                     /tool="antismash"
     gene            1910..2713
                     /db_xref="GeneID:95687525"
                     /locus_tag="H4W23_RS03080"
                     /old_locus_tag="H4W23_03070"
     CDS             1910..2713
                     /codon_start=1
                     /db_xref="GeneID:95687525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031432.1"
                     /locus_tag="H4W23_RS03080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_03070"
                     /product="endonuclease I family protein"
                     /protein_id="WP_055645269.1"
                     /transl_table=11
                     /translation="MSVVRIGRWKAWAAALAATLMSVTLPAITATPASATTTAYDSTYY
                     KNAVGKTGTSLKSSLHTIISSQTRISYDAVWNALKVTDQDPANTNNVVLLYSGVSRSKS
                     LNGGAVGDWNREHVWAQSHGNFGTSAGPGTDLHHLRACDVQVNSTRGNKDWDNGGSAVS
                     GAAGSYTDSNSFEPRNADKGDVARMILYMAVRYEGDDAWADLEPNESTSNGSVPWHGRL
                     SILKQWNEQDPPSAFEERRNDIIHSTYQGNRNPFIDHPEWVEAIW"
     gene            3078..3590
                     /db_xref="GeneID:95687526"
                     /gene="ectA"
                     /locus_tag="H4W23_RS03085"
                     /old_locus_tag="H4W23_03075"
     CDS             3078..3590
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95687526"
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006375120.1"
                     /locus_tag="H4W23_RS03085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_03075"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_055645268.1"
                     /transl_table=11
                     /translation="MTTATTARTTPPTIADGAELWRIAHGSGELDLNSPYSYLLWCRDF
                     ADTTSVARDTSGRPIGFVTGYLRPDAPKTLFVWQIAVEGSHRGSGVAGTLLDALTARVA
                     TEHGIDRVEATVTPGNVASDRLFRSYARRQGADVTREVLFPSAAFPAAGHDSEVLYRIG
                     PIGPPAS"
     gene            3678..4949
                     /db_xref="GeneID:95687527"
                     /gene="ectB"
                     /locus_tag="H4W23_RS03090"
                     /old_locus_tag="H4W23_03080"
     CDS             3678..4949
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (30-366). E-value: 1.8e-74.
                     Score: 242.7. Matches aSDomain:
                     nrpspksdomains_H4W23_RS03090_Aminotran_3.1"
                     /codon_start=1
                     /db_xref="GeneID:95687527"
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031434.1"
                     /locus_tag="H4W23_RS03090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_03080"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_196479240.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.7e-77, bitscore:
                     259.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATTDIAAPSVFETLESEVRSYCRAWPVVFARATGSRLYDEQGRP
                     YLDFFAGAGSLNYGHNNPALKRALLDYLADDGITHGLDMSTTAKRDFLETFHSTVLEPR
                     GLPYKVMFPGPTGTNAVEAALKLARKVTGRQTVVSFTNAFHGMSLGSLAVTGNAAKRAG
                     AGVPLHHAIRMPFDDYLDGHLPDFLWFERLLDDAGSGLDHPAAVIVETVQGEGGINVAR
                     AEWLRALAELCRRHDMLLIADDIQMGCGRTGDFFSFEEAGITPDIVTLSKSISGYGLPM
                     ALCLFRPELDVWQPGEHNGTFRGNNPAFVTATAALDAYWHDAALRERTLGRADRVERAL
                     LDLCGGDERTGLSVRGRGLVWGLEFKDGERADAVCRRAFETGLLVETSGPRGEVVKLLP
                     PLTVTDDELDEGLGILARSVRHTA"
     aSDomain        3768..4775
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_H4W23_RS03090_Aminotran_3.1"
                     /evalue="1.80E-74"
                     /label="H4W23_RS03090_Aminotran_3.1"
                     /locus_tag="H4W23_RS03090"
                     /protein_end="366"
                     /protein_start="30"
                     /score="242.7"
                     /tool="antismash"
                     /translation="ARATGSRLYDEQGRPYLDFFAGAGSLNYGHNNPALKRALLDYLAD
                     DGITHGLDMSTTAKRDFLETFHSTVLEPRGLPYKVMFPGPTGTNAVEAALKLARKVTGR
                     QTVVSFTNAFHGMSLGSLAVTGNAAKRAGAGVPLHHAIRMPFDDYLDGHLPDFLWFERL
                     LDDAGSGLDHPAAVIVETVQGEGGINVARAEWLRALAELCRRHDMLLIADDIQMGCGRT
                     GDFFSFEEAGITPDIVTLSKSISGYGLPMALCLFRPELDVWQPGEHNGTFRGNNPAFVT
                     ATAALDAYWHDAALRERTLGRADRVERALLDLCGGDERTGLSVRGRGLVWGLEFK"
     gene            5001..5420
                     /db_xref="GeneID:95687528"
                     /locus_tag="H4W23_RS03095"
                     /old_locus_tag="H4W23_03085"
     CDS             5001..5420
                     /codon_start=1
                     /db_xref="GeneID:95687528"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031435.1"
                     /locus_tag="H4W23_RS03095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_03085"
                     /product="ectoine synthase"
                     /protein_id="WP_055645267.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.1e-51, bitscore:
                     171.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFDELENTERHVRAASGTWESKRIVLARERVGFSLHETVLY
                     AGTETSMWYAHHVEAVVCTAGEAELTDLETGRSYTIVPGTMYLLDGHERHTLKVKRDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEPGRAAGHDV"
     gene            5506..6402
                     /db_xref="GeneID:95687529"
                     /gene="thpD"
                     /locus_tag="H4W23_RS03100"
                     /old_locus_tag="H4W23_03090"
     CDS             5506..6402
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95687529"
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031436.1"
                     /locus_tag="H4W23_RS03100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_03090"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_055646539.1"
                     /sec_met_domain="PhyH (E-value: 1.2e-35, bitscore: 122.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASAPTRPVDLYPTRGPKEELIGRKDPVVWSEPGTAGPFWARELA
                     EYDRDGFVTVDELVTPAEVDELRAELDRLVTDPAVRADERAIVEPRSQEIRSVFEVHRL
                     SEVFGRLAEDPRVVGRARQILGSDVYVHQSRVNVKPGFGASGFFWHSDFETWHAEDGLP
                     RMRTVSVSIALTANHTTNGSLMIMPGSHRTFLGCAGATPRDNYKQSLRMQDAGTPSHEA
                     LTTFADACGIRHFTGPAGSATWFDCNALHGSGDNITPYPRSNVFLVFNSVENQPEAPFA
                     APVRRPSFVAARPGGRS"
     gene            6399..7316
                     /db_xref="GeneID:95687530"
                     /gene="ehuB"
                     /locus_tag="H4W23_RS03105"
                     /old_locus_tag="H4W23_03095"
     CDS             6399..7316
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95687530"
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356096.1"
                     /locus_tag="H4W23_RS03105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_03095"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_055645266.1"
                     /transl_table=11
                     /translation="MTGPAPLDRRRFLGRTALLGGLALTPGLLAACTRTQVGTGAPEDD
                     GALLARLRKQGFVRVGFAGEAPYGFQDGAELAGEAPTLHREIFTALGVRELRPTLTEFG
                     ALIPGLLADRFDVVSAGMSITPERCAKVIFSEPEFVSPTALMVRAGNPKGLSDLASCAA
                     KGATVGVLTAAVEASFAKAAGVPEGSVKTLAKQQDGLDALLAGRIDAFALTAISLRWLA
                     RTNRGASVEVAEPFVPVVDGVRQLGAGGAVFRPGATGLRDAFDTELRKITGAPDRFVEL
                     IGRYGFTTREVPPRSLRTADLCTG"
     gene            7329..8003
                     /db_xref="GeneID:95687531"
                     /gene="ehuC"
                     /locus_tag="H4W23_RS03110"
                     /old_locus_tag="H4W23_03100"
     CDS             7329..8003
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95687531"
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455303.1"
                     /locus_tag="H4W23_RS03110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_03100"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_055645265.1"
                     /transl_table=11
                     /translation="MADFLPELGRAAPRLADGLLVTVEATVLGAALALVLAYALGLLSR
                     SARRPVRGAARLVVEFFRGTSLYVQLFWLFFALPMLGFRLEPLACGVLALGLNFGAYGS
                     EVVRGAVEAVPRAQTEAAIALGMGPALQLRRVVLPQAHALMVAPFKNLLVQLLKATPLL
                     SLVTVPDLTFQIDQLRSSTGATAASYLLLLGLYFGLAVVLSLAMNALERAAKARAGQEG
                     GV"
     gene            8003..8638
                     /db_xref="GeneID:95687532"
                     /gene="ehuD"
                     /locus_tag="H4W23_RS03115"
                     /old_locus_tag="H4W23_03105"
     CDS             8003..8638
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95687532"
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563990.1"
                     /locus_tag="H4W23_RS03115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_03105"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_055645264.1"
                     /transl_table=11
                     /translation="MWDGEAALAALPAVLEGFGVTLVATVPAFALAAVLGLALALVQRA
                     GPAWLALPVRGAAGFVRSTPLLVQLFAAWVLVPGLDAPVLGVLVLGVHYATYLSEVYRT
                     GIDAVPKGQWEACTALSLPRRRVWRAVVLPQAVRNVLPPLGNYAVSMFKETPLLSVITV
                     HEMVHEANTYGSTHFAYLESFTLAAAVFLLASWPTSALLRRLEARLAH"
     gene            8628..9407
                     /db_xref="GeneID:95687533"
                     /gene="ehuA"
                     /locus_tag="H4W23_RS03120"
                     /old_locus_tag="H4W23_03110"
     CDS             8628..9407
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95687533"
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009068407.1"
                     /locus_tag="H4W23_RS03120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_03110"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_055645263.1"
                     /transl_table=11
                     /translation="MPTEDPAVRFDRVTKRYGDHTVLDGLDLEVARGERVTLIGPSGSG
                     KTTILRLLMTLERVTDGVIHVAGQPFSHMPGRTPGKLVPASERHLAGRRRRIGMVFQQF
                     NLFPHMSVLDNVVEAPVHVLGESRDEAAHRARDLLDLVGLADRVDARPTRLSGGQQQRV
                     AIARALAMRPEILLLDEVTSALDPELVAEVLDVLRDVARRTDITLLCVTHEMGFARDVS
                     DRILMFDRGRIVESGPADALLDAPAEERTRAFLGSVR"
     gene            complement(9431..10303)
                     /db_xref="GeneID:95687534"
                     /locus_tag="H4W23_RS03125"
                     /old_locus_tag="H4W23_03115"
     CDS             complement(9431..10303)
                     /codon_start=1
                     /db_xref="GeneID:95687534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015031437.1"
                     /locus_tag="H4W23_RS03125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H4W23_03115"
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="WP_055645262.1"
                     /transl_table=11
                     /translation="MRTDPAEATTTYATNGVGEDLPFPEVPHEVPPRDARALSKVFFRE
                     LAAREEGTAGHQYVRNTLIEMNMSLVVYAAGRFRTGGPEREDILQVGMVGLIKAIDRFD
                     LSREVEFTTFAVPYIVGEIKRFFRDTTWAVHVPRRLHEARVELAKATEELSSRLGRAPR
                     IAELAPAMDLSEDEVIEAQVAANGYHSTSLDATFAGGHGEDEDAALADLIGEEDPALAL
                     FEEFHTLAPLVEALPPRDRLLLHLRFVEELTQTQIGERLGVSQMHVSRLLARSLDRLRD
                     GMIEEGGEG"
ORIGIN
        1 tgacgacggc cgaggccgac cgggagccgc cggtggcgag cgcgggctcc gcccggaccg
       61 gtccctcaag gaggcggcgc aggagagcgg tgcggcgcgc ggggtccgtg gcgccgtcac
      121 cctccgcgag gagcgcggcg aggacggcga tccgcgcctg ggcggcccgg agcgtaccgg
      181 tgagcagcgc gccggcggcg acgaggtcga ccgctccgcc gccggtgtag atctcggcga
      241 gcgggcccgc gggcacgcgg gtgctcacgg cgacgaggac gccctgggcg accgcgtccg
      301 cgacggcggc cgtgatctcg ggggtggcgt tgcccgcgcc ggtcgcttcg aggacgatgc
      361 cccgggctcc ggcggccagt gcggcccgga tgaagagcgg gtcgccgtcg gcgtggtgcg
      421 tgacgatgtc gacccgaggc agcggggtcg cgccggggtc ggtggcaccg ggggtcacct
      481 tcagcgcggc gtcggggagc gacagcgggg cggggcgctc cgggaggcgc tcgacgtcga
      541 cgcgggagaa gccgacgcgg ccgaggcgct cggcggaggg gtccgagaag gcgtcggcgg
      601 cgagggtctg ggtcttgacc gtgccgcggg ccgcgtggac gcgtccgtcg aagacgacga
      661 ggacgcccag ctcgcggacg gaggcggcga cctggagggc gtcgtacagg ttcccggggc
      721 cgtcgccgtc gcctgtaccg aaggggcgct gggcgccggt caggacgacc ggacgggcgt
      781 cgctgtggtg gaggtccagg aggaaggcgg tctcctccag cgtgtcggtg ccgtgggtga
      841 ccacgatgcc gtcgacgccg gggtcggcga acgtctcgtg gacggtccgc aggagggcga
      901 gctggtgggc cgaggtcatg cgcgagctgt tcacgttgaa caggtcgacg acctcgacgg
      961 tgacgccctc ggggaggggt gcggtggcca ggacgtcgct gccggaggca tcggccgcgt
     1021 agcccgtgcc ctgccagcgg ctggcgatcg tgccgccggt gctgatgacg acgatccggc
     1081 gcggtcctcc gtcgttcgtc cgcatcattg cctgccgtcc ttactcgtac cgaagccacg
     1141 actcgcgccg aggccacgaa ccggaatcac cctgctccgc aacgatagga caggaggcac
     1201 gcaagccgat tgcgaaatgg gccgcaggaa tgtttcatcg tggtagataa ttgcgccatg
     1261 gaccgaatcg atctccacat cttgcgcgag ctccagaacg acggccgcct gagcaaccag
     1321 gagctggccc agcgggtcgg cctgagcccc tccccctgtc tgcgccgggt gcgccagctg
     1381 gagcaggacg gggtgatcca gggctaccgg gcgatcatcg acccggaggc ggtggggcgg
     1441 ggcttcgagg tgctcgtctc ggtcgaggtg cggcgggacc gcgagacggt ggaggccttc
     1501 gaggaagcgc tccaggacgt gccggacgtc atcgaggcgt accgactgtt cggcagtccc
     1561 ggttgcctgc tgcgcatcgc ggtggccgac ctcgcggcgt acgagcggtt gtggatcgag
     1621 cggctgacga cgctggccgg ggtcaccgag gtcaactcgc agatcatcat gaagcgcatc
     1681 aaggagccga aggggatgcc tgtggacgtc cggggcgcgt gaggacgctt cggcgccgtt
     1741 cggttcctcc atatgcgaga acgtcacgga acagcctctt ccccgttaca tgacaggtac
     1801 ctgacctgtg tcaccctccc gatgacacga agtcaacacg cgtagacagc ggaagccttg
     1861 agccggtttc cgccgcaccg cgcgtatcgt ccccgggaaa gggcccccca tgtccgttgt
     1921 gcggatcggt agatggaagg cgtgggcggc ggccctcgcc gccaccctca tgagcgtcac
     1981 gctccccgcg atcaccgcga cgcccgccag tgccacgacg accgcgtacg acagcacgta
     2041 ctacaagaac gcggtcggca agaccggcac gagcctcaag tcctcgctgc acacgatcat
     2101 cagcagccag acgaggatct cgtacgacgc cgtctggaac gcgctgaagg tgaccgacca
     2161 ggatcccgcc aacaccaaca acgtggtcct tctctacagc ggcgtctcgc gcagcaagtc
     2221 gctcaacggc ggcgccgtgg gcgactggaa ccgcgagcac gtgtgggcgc agtcccacgg
     2281 caacttcggc acctcggccg ggccgggcac ggacctgcac cacctgcggg cgtgtgacgt
     2341 ccaggtcaac agcacccgcg ggaacaagga ctgggacaac ggcggcagcg cggtcagcgg
     2401 cgccgccggc agctacaccg actccaactc cttcgagccg cgcaacgccg acaagggcga
     2461 cgtggcccgc atgatcctct acatggccgt ccgctacgag ggcgacgacg cctgggccga
     2521 cctggagccc aacgagtcga cctccaacgg cagcgtcccc tggcacggcc gcctctcgat
     2581 cctgaagcag tggaacgagc aggacccgcc gagcgccttc gaggagcgcc gcaacgacat
     2641 catccacagc acctaccagg gcaaccgcaa ccccttcatc gaccaccccg agtgggtcga
     2701 ggcgatctgg taggtccagc gcgaccgaac gaacggagcc gggccgggct cgtgacaccg
     2761 gcccggctcc tgcgcgttcc gggggtgttc accgcggggg cgcacacgga tcgacccgta
     2821 cgcctgaccg ttcccggcgg cttcaaacgc gaaatcccgg atcttctcac gctcttcgcg
     2881 cacgcgtcag gggtgcgtga gagacgttgc acatccgtta taaacggtga tttcgccccg
     2941 ctacgaccct gttttccgtg cgccgaagca tccattcccg ccacaccgac ctccggcgca
     3001 tctatcacgt tcctcctgtc tcgaacaagg cctccggacc aggaagttcc gacttccact
     3061 gggtagcttc ggcgcccatg accacagcga ccactgcccg cacgacaccg ccgacgatcg
     3121 cggacggcgc cgaactctgg cggatcgccc acggctcggg ggaactggac ctcaactcgc
     3181 cgtacagcta tctgctgtgg tgccgcgact tcgccgacac gacctccgtc gcccgtgaca
     3241 cctccgggcg cccgatcgga ttcgtcaccg gctacctgcg gccggacgcg ccgaagaccc
     3301 tcttcgtctg gcagatcgcc gtcgagggtt cgcaccgcgg aagcggggta gccggaaccc
     3361 tgctggacgc cctgaccgca cgtgtggcga ccgagcacgg aatcgaccgg gtcgaggcga
     3421 ccgtcacccc gggaaacgtg gcgtccgacc gcctgttccg gtcctacgcg cgccgacagg
     3481 gcgcggacgt gacgagggag gtcctcttcc cgtcggccgc cttccccgcg gcgggccacg
     3541 actccgaggt gctgtaccgc atcggcccga tcggtccccc ggcgtcctga gcgctcgccc
     3601 gtcgcccccg tacgccccgc cgctcgcgta cgccccgccc ccccctctgg agaagagact
     3661 tcgtgaccct gaccgacatc gccacgaccg acatcgccgc cccctccgtc ttcgagaccc
     3721 tcgaatcgga ggtccgcagc tactgccgcg cctggcccgt tgtcttcgcg cgcgccaccg
     3781 gcagccggct gtacgacgag caggggcgcc cgtacctcga cttcttcgcc ggcgcgggct
     3841 ccctgaacta cggccacaac aatccggcgc tcaagcgggc cctcctcgac tacctcgccg
     3901 acgacgggat cacccacggt ctcgacatgt cgacgaccgc caaacgggac ttcctggaga
     3961 ccttccactc cacggtcctg gagccccgcg gcctgcccta caaggtgatg ttccccggcc
     4021 ccacgggcac caacgccgtg gaggccgcgc tgaagctcgc ccggaaggtc accgggcgac
     4081 agacggtcgt ctccttcacc aacgccttcc acgggatgtc gctcggctcg ctcgccgtca
     4141 ccggcaacgc cgccaagcgc gccggtgcgg gcgtccccct ccaccacgcc atccgcatgc
     4201 ccttcgacga ctacctcgac gggcacctcc ccgacttcct ctggttcgag cggctcctgg
     4261 acgacgccgg ctccggcctc gaccacccgg cggccgtgat cgtcgagacg gtccaggggg
     4321 aaggcggtat caacgtcgcc cgggccgagt ggctgcgcgc gctcgcggag ctctgccgcc
     4381 ggcacgacat gctcctcatc gcggacgaca tccagatggg ctgcgggcgc accggtgact
     4441 tcttctcctt cgaggaggcg ggcatcacgc ccgacatcgt caccctgtcc aagtccatca
     4501 gcggctacgg cctgcccatg gccctctgcc tcttccggcc cgagctcgac gtgtggcagc
     4561 ccggcgaaca caacggcacc ttccgcggca acaaccccgc cttcgtcacc gccacggccg
     4621 ccctcgacgc ctactggcac gacgccgccc tgcgcgagcg caccctgggc cgtgccgacc
     4681 gggtcgagcg cgcgctcctc gacctgtgcg ggggcgacga gcgcaccggc ctctcggtcc
     4741 ggggccgcgg gctggtgtgg ggcctggagt tcaaggacgg ggagcgcgcc gacgcggtgt
     4801 gccgccgcgc cttcgagacc ggcctgctcg tggagacctc ggggccgcgc ggcgaggtcg
     4861 tcaagctcct gccgccgctg accgtgacgg acgacgaact cgacgagggc ctcggcatcc
     4921 tggcccgctc cgtccgtcac accgcctgac cctcgccgcg cttccaccgg caacagcacg
     4981 catccgaaag gcgacacccc atgatcgtcc gttccttcga cgagctggag aacaccgagc
     5041 ggcacgtgag ggccgcgtcg ggcacctggg agagcaagcg catcgtcctg gcccgcgagc
     5101 gggtcggctt ctccctccac gagacggtgc tgtacgccgg caccgagacc tccatgtggt
     5161 acgcgcacca cgtcgaggcc gtcgtgtgca cggccggcga ggccgagctc accgacctgg
     5221 agacgggccg ctcgtacacg atcgtgcccg gaacgatgta cctgctcgac gggcacgagc
     5281 gccacaccct caaggtcaag cgggacttcc gctgcctgtg cgtcttcaac ccacctgtca
     5341 cgggccgcga ggaccacgac gagaacggcg tctaccccct gctcaccgaa cccggccggg
     5401 cggccgggca cgacgtctga gccacggcac gaaccccacg cgagatccgt acgcaccccc
     5461 gagagatccg tacgcatgac cgagagaaag gcgacacgct ccctcatggc ctccgcaccc
     5521 acccgccccg tcgacctcta ccccacccgt ggccccaagg aggaactgat cggccgcaag
     5581 gaccccgtcg tgtggtccga gcccggcacg gcgggcccct tctgggcccg tgaactggcg
     5641 gagtacgacc gcgacggctt cgtcacggtg gacgagctcg tcactccggc cgaggtcgac
     5701 gagctccggg cggagctgga ccggctcgtc accgaccccg ccgtgcgggc ggacgagcgc
     5761 gcgatcgtcg agccccgctc ccaggagatc cggtccgtct tcgaggtgca ccggctcagc
     5821 gaggtcttcg gccggcttgc cgaggacccc cgggtcgtcg gccgcgcccg ccagatcctc
     5881 ggctccgacg tctacgtcca ccagtcgcgg gtcaacgtga agcccggctt cggcgccagc
     5941 ggcttcttct ggcactccga cttcgagacc tggcacgcgg aggacggcct gccgaggatg
     6001 cggaccgtct cggtgtcgat cgcgctcacg gcgaaccaca cgaccaacgg cagcctgatg
     6061 atcatgccgg gctcgcatcg cacgttcctc ggctgcgccg gtgccacgcc ccgggacaac
     6121 tacaagcagt cgctccgcat gcaggacgcc ggcaccccgt cccacgaggc gctgaccacc
     6181 ttcgccgacg cctgcggcat ccgccacttc accggtccgg cgggctcggc cacctggttc
     6241 gactgcaacg ccctgcacgg ctcgggcgac aacatcaccc cgtacccccg cagcaatgtg
     6301 ttccttgtct tcaacagcgt cgagaaccag cccgaggcgc ccttcgccgc cccggtccgg
     6361 cggccctcct tcgtcgccgc ccgccccggg ggccggtcat gaccggcccc gcgcccctcg
     6421 accgccgccg cttcctcggc agaacggcgc tcctgggcgg actcgccctg acgcccggcc
     6481 tcctggcggc gtgcacccgg acccaggtgg ggacgggggc gcccgaggac gacggcgcgc
     6541 tcctggcgag gctgcggaag cagggcttcg tgcgggtcgg cttcgccggc gaggcgccgt
     6601 acggcttcca ggacggcgcc gagctcgccg gcgaggcccc gacgctccac cgcgagatct
     6661 tcacggccct cggagtacgc gaactgcgcc cgacgctgac cgagttcggg gcgctgatcc
     6721 ccgggctcct ggccgaccgg ttcgacgtgg tgagcgcggg catgtcgatc acgcccgagc
     6781 gctgcgcgaa ggtgatcttc tccgagccgg agttcgtctc ccccaccgcc ctgatggtcc
     6841 gcgcgggcaa tccgaaggga ctgagcgacc tcgcgtcctg cgcggcgaag ggcgccaccg
     6901 tcggcgtgct gaccgccgcc gtggaggcgt ccttcgcgaa ggcggcggga gtcccggaag
     6961 ggtcggtgaa gaccctggcc aagcagcagg acgggctcga cgccctcctg gccgggcgga
     7021 tcgacgcctt cgcgctgacc gccatctccc tgcgctggct cgcgcggacg aaccggggtg
     7081 cctcggtcga ggtggccgag ccgttcgtcc cggtggtcga cggcgtacgg caactcggcg
     7141 cgggaggagc ggtgttccgg cccggggcga ccgggctgcg ggacgccttc gacaccgagc
     7201 tccggaagat caccggggcc ccggaccggt tcgtggagct catcgggcgc tacggcttca
     7261 ccacgcgcga ggtgcctccc cgttccctgc ggaccgccga cctgtgcaca gggtgagggg
     7321 cggtcggcat ggctgacttc ctccccgagc tcggccgggc ggcgccccgg ctcgccgacg
     7381 gactgctcgt cacggtcgag gccacggtgc tcggcgccgc gctcgccctg gtcctcgcgt
     7441 acgccctcgg cctgctgtcc cggtcggcgc ggcggccggt gcgcggagcg gcccggctcg
     7501 tcgtcgagtt cttccgcgga acgtcgctgt acgtgcagct cttctggctg ttcttcgcgc
     7561 tgccgatgct cgggttccgg ctggagccgc tggcctgcgg cgtgctcgcg ctcggcctca
     7621 acttcggggc gtacggctcc gaggtggtgc ggggcgcggt cgaggcggtg ccccgcgccc
     7681 agaccgaggc ggcgatcgcg ctgggcatgg gcccggcact gcagctgcgc cgggtcgtgc
     7741 tgccgcaggc gcacgccctg atggtcgcgc cgttcaagaa cctgctcgtc cagctgctga
     7801 aggcgacgcc gctgctctcc ctggtcacgg tccccgacct gaccttccag atcgaccagc
     7861 tgcgctcgtc gacgggggcg acggccgcct cgtacctgct gctcctgggg ctgtacttcg
     7921 gcctggccgt cgtgctgagc ctcgccatga acgccctgga gcgggccgcg aaggcccgtg
     7981 ccgggcagga aggaggcgtg tgatgtggga cggggaggcg gccctggccg ctctgccggc
     8041 cgtgctcgaa gggttcggcg tcaccctggt cgcgaccgtg ccggccttcg ccctcgcggc
     8101 ggtcctgggt ctcgcgctcg cgctcgtcca gcgcgccggt ccggcctggc tggccctgcc
     8161 ggtgcgcggg gcggccgggt tcgtccgctc gaccccgctc ctggtgcagc tgttcgcggc
     8221 ctgggtgctg gtgccgggcc tcgacgcgcc ggtgctcggc gtgctggtcc tgggcgtgca
     8281 ctacgccacg tacctctccg aggtgtaccg gacgggcatc gacgccgtac cgaaggggca
     8341 gtgggaggcc tgtacggccc tgtcgctgcc gcgccggcgg gtgtggcggg cggtggtgct
     8401 gccgcaggcc gtgcgcaacg tgctgccgcc gctggggaac tacgcggtgt ccatgttcaa
     8461 ggagacgccg ctgctgtcgg tgatcacggt gcacgagatg gtccacgagg cgaacacgta
     8521 cggcagcacc cacttcgcct acctggagag cttcaccctg gccgccgcgg tgttcctgct
     8581 cgcgagctgg ccgacgtccg cgctgctacg acgactggag gcacgccttg cccactgagg
     8641 acccggcggt ccgcttcgac cgcgtcacga agcggtacgg cgaccacacc gtgctcgacg
     8701 gcctcgacct ggaggtcgcc cgcggggagc gggtgaccct catcggcccg agcggctccg
     8761 ggaagaccac gatcctccgg ctgctcatga cgctggaacg ggtcacggac ggggtcatcc
     8821 acgtggccgg acagcccttc tcccacatgc cgggccgaac gcccgggaag ctggtgccgg
     8881 cgagcgaacg ccatctggcc gggcgacggc ggcggatcgg catggtcttc cagcagttca
     8941 acctcttccc gcacatgagc gtcctcgaca acgtcgtcga ggcgcccgtg cacgtcctcg
     9001 gcgagagcag ggacgaggcc gcgcaccggg cgcgggacct cctcgacctg gtcgggctcg
     9061 ccgacagggt cgacgcgcgg ccgacccggc tctccggcgg ccagcagcag cgggtggcca
     9121 tcgcccgcgc gctcgcgatg cgccccgaga tcctgctcct ggacgaggtg acgtccgcgc
     9181 tcgacccgga gctggtggcg gaggtgctcg acgtgctgcg ggacgtcgcc cggcggaccg
     9241 acatcaccct cctgtgcgtg acgcacgaga tgggcttcgc gcgggacgtc tcggaccgga
     9301 tcctgatgtt cgaccgcggc cggatcgtcg agtcgggacc ggcggacgcg ctcctggacg
     9361 cgcccgccga ggagcgcacc cgcgcgttcc tgggctcggt ccgctgagat caccggaccc
     9421 ggaccgtcct tcagccctca ccgccctcct cgatcatgcc gtcccgcaac cggtcgaggc
     9481 tccgggcgag cagccgtgag acgtgcatct gggagacgcc gagccgctca ccgatctgcg
     9541 tctgggtgag ctcctccacg aagcgcaggt gcagcagcag ccggtcgcgg ggcgggaggg
     9601 cctccacgag cggggcgagg gtgtggaact cctcgaagag cgcgagcgcc gggtcctcct
     9661 cgccgatgag gtcggccagg gccgcgtcct cgtcctcgcc gtggccgccg gcgaacgtgg
     9721 cgtccaggga cgtggagtgg tagccgttgg cggcgacctg ggcctcgatc acctcgtcct
     9781 cggacaggtc catggccggc gccagctccg cgatgcgcgg ggcccggccg agccgtgagc
     9841 tcagctcctc ggtcgccttg gccagctcca cccgcgcctc atggaggcgg cgcgggacgt
     9901 gcacggccca ggtggtgtcg cggaagaagc gcttgatctc acccacgatg tacggcacgg
     9961 cgaaggtggt gaactccacc tcgcgcgaca ggtcgaagcg gtcgatcgcc ttgatgagac
    10021 cgaccatgcc gacctggagg atgtcctccc gctcgggacc gcccgttcgg aaccggcccg
    10081 ccgcgtacac caccagtgac atgttcatct cgatgagcgt gttccggacg tactggtggc
    10141 ccgcggtgcc ctcctcccgc gccgccagtt cgcggaagaa caccttcgac agggcacgtg
    10201 cgtcccgggg tggcacctcg tgcggcacct cgggaaaggg caggtcctcg cccacgccgt
    10261 tcgtcgcata cgtcgtcgtc gcctcggccg gatcggtccg cacggggaca acctcctctg
    10321 gctcgcggtc cgttcagcgg tcccggttct cgtacccccg cttcccgaag gcactcctcg
    10381 tccgatcccg accgattccg cccggaagat tccacccgga
//
