LOCUS       NZ_CP065054            10195 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Streptococcus equi subsp. zooepidemicus strain SEZ13 chromosome,
            complete genome.
ACCESSION   NZ_CP065054
VERSION     NZ_CP065054.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus equi subsp. zooepidemicus
  ORGANISM  Streptococcus equi subsp. zooepidemicus
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2150055)
  AUTHORS   Borlee,G.I., Lakin,S.M., Kapuscinski,M.L., Abdo,Z., Stenglein,M.D.,
            McCue,P.M. and Borlee,B.R.
  TITLE     Complete genomes of eight Streptococcus equi subsp. zooepidemicus
            strains isolated from mares in estrus with endometriti
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2150055)
  AUTHORS   Borlee,G.I., Lakin,S.M., Kapuscinski,M.L., Abdo,Z., Stenglein,M.D.,
            McCue,P.M. and Borlee,B.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2021) Microbiology, Immunology, and Pathology,
            Colorado State University, 2025 Campus Delivery, Fort Collins, CO
            80523, USA
  REMARK    Annotation added by submitter
REFERENCE   3  (bases 1 to 2150055)
  AUTHORS   Borlee,G.I., Lakin,S.M., Kapuscinski,M.L., Abdo,Z., Stenglein,M.D.,
            McCue,P.M. and Borlee,B.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2020) Microbiology, Immunology, and Pathology,
            Colorado State University, 2025 Campus Delivery, Fort Collins, CO
            80523, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Coverage                   :: 246
            Sequencing Technology :: Illumina NextSeq;Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015689395.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 02:47:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 1,970
            Genes (coding)                    :: 1,933
            CDSs (with protein)               :: 1,933
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 16 of 37
            Pseudo Genes (incomplete)         :: 20 of 37
            Pseudo Genes (internal stop)      :: 16 of 37
            Pseudo Genes (multiple problems)  :: 14 of 37
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:11:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1747094
            Orig. end                         :: 1757289
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065054.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10195
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10195
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10195
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5195](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5195)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(475..486)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.4"
                     /tool="antismash"
     misc_feature    517..530
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.29"
                     /tool="antismash"
     gene            complement(825..1469)
                     /db_xref="GeneID:83705463"
                     /gene="fsa"
                     /locus_tag="I2432_RS08250"
                     /old_locus_tag="LJFMMFNO_01648"
     CDS             complement(825..1469)
                     /GO_function="GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83705463"
                     /gene="fsa"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /locus_tag="I2432_RS08250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_01648"
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="WP_012677466.1"
                     /transl_table=11
                     /translation="MKFFLDTANVEAIRAINELGVVDGVTTNPSIISREGRDFETVIKE
                     ICEIVDGPISAEVTGLTAEEMIAEARSIAKWHDNVVVKIPMTTEGLKATNVLSQEGIKT
                     NVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGADPYQLISDLRGIIDLYGFQAEIIAA
                     SIRTAAHVEAVAQLGAHIATIPDPLFAKMTEHPLTTNGLKTFMEDWASFKQ"
     misc_feature    complement(1520..1538)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 24.22"
                     /tool="antismash"
     gene            complement(1644..3149)
                     /db_xref="GeneID:83705464"
                     /gene="glpK"
                     /locus_tag="I2432_RS08255"
                     /old_locus_tag="LJFMMFNO_01649"
     CDS             complement(1644..3149)
                     /EC_number="2.7.1.30"
                     /GO_function="GO:0004370 - glycerol kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83705464"
                     /gene="glpK"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1210: glycerol kinase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516190.1"
                     /locus_tag="I2432_RS08255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_01649"
                     /product="glycerol kinase GlpK"
                     /protein_id="WP_111678443.1"
                     /transl_table=11
                     /translation="MGTETYIMAIDQGTTSSRAIIFNKKGEALVSSQKEFPQFFPQAGW
                     VEHNANHIWNSVQSVIADAFIESGIKPEQIEAIGITNQRETTVVWDKQTGLPIYNAIVW
                     QSRQTASIAEQLKRDGYTKMIHEKTGLVIDAYFSATKLRWLLDHVPGAQERAERGELLF
                     GTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKELKWDDEILALLNIPKAMLPEVRSNS
                     EIYGKTAPFHFYGGEVAIAGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNTGQEAQ
                     LSSSNLLTTIGYGINGKVYYALEGSIFIAGSAVQWLRDGLRMIKTSFESEQLALASTNA
                     DEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTTKEDFVKATLQSIAYQVRDVIDTMQVD
                     SGITIQQLRVDGGAAMNNMLMQFQADILGIDIARAENLETTALGAAFLAGLAVGYWEDM
                     GTLKQLNATGQLFKASMSNSRREKLYQGWKRAVKATQFFTEEL"
     misc_feature    complement(3284..3299)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 23.65"
                     /tool="antismash"
     gene            complement(3483..4076)
                     /db_xref="GeneID:83705465"
                     /locus_tag="I2432_RS08260"
                     /old_locus_tag="LJFMMFNO_01650"
     CDS             complement(3483..4076)
                     /codon_start=1
                     /db_xref="GeneID:83705465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677464.1"
                     /locus_tag="I2432_RS08260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_01650"
                     /product="PepSY domain-containing protein"
                     /protein_id="WP_111678445.1"
                     /transl_table=11
                     /translation="MIIKKLSVIALTSLSLISLSACRQQNQALGQNGSAQNHRKASVSE
                     DKAKELAFKDASITAADAQRLKLTKETDDGRVVYDIEFDAKDQEYSYTIDANTGEILEK
                     SSEPITEAPASAQAAGGQADKAAGISEDKAKELAFKDASITAADAQRLKLTKETDDGRL
                     VYDIEFDAKDQEYSYTIDANSGDILEKSSEPVYD"
     misc_feature    4288..4303
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.1"
                     /tool="antismash"
     gene            complement(4742..4942)
                     /db_xref="GeneID:83705466"
                     /locus_tag="I2432_RS08265"
                     /old_locus_tag="LJFMMFNO_01651"
     CDS             complement(4742..4942)
                     /codon_start=1
                     /db_xref="GeneID:83705466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /locus_tag="I2432_RS08265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_01651"
                     /product="bacteriocin"
                     /protein_id="WP_043041044.1"
                     /transl_table=11
                     /translation="MEHYRALAINEMQDISGGNAAGNAVIGGLGGLKTGIKFCKIPHPV
                     LKGVCIAGFTAGGAYLAYKAN"
     gene            complement(5001..5195)
                     /db_xref="GeneID:83705467"
                     /locus_tag="I2432_RS08270"
                     /old_locus_tag="LJFMMFNO_01652"
     CDS             complement(5001..5195)
                     /codon_start=1
                     /db_xref="GeneID:83705467"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516193.1"
                     /locus_tag="I2432_RS08270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_01652"
                     /product="bacteriocin"
                     /protein_id="WP_111678447.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.2e-07,
                     bitscore: 28.0, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATEMLASFEREKNNWAANVSGVIGAGSAGAALGFPVCGVACGSI
                     GAKTGVTLWTWATGVTGGF"
     gene            complement(5700..6566)
                     /db_xref="GeneID:83705468"
                     /gene="rsmI"
                     /locus_tag="I2432_RS08275"
                     /old_locus_tag="LJFMMFNO_01653"
     CDS             complement(5700..6566)
                     /EC_number="2.1.1.198"
                     /GO_function="GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83705468"
                     /gene="rsmI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TP_methylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680028.1"
                     /locus_tag="I2432_RS08275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_01653"
                     /product="16S rRNA (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="WP_111678449.1"
                     /sec_met_domain="TP_methylase (E-value: 1.1e-22, bitscore:
                     77.9, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQIQKSFKEEKESGVLYLVPTPIGNLEDMTFRAVNVLKEVDIICA
                     EDTRNTGLLLKHFGIKTKQVSFHEHNAYDKIPDLLDLLLAGRSLAQVSDAGMPSISDPG
                     HELVRAAIDRDITIVALPGASAGITGLIASGLVPQPHLFYGFLPRKAGQQRAFLMTKTD
                     YPETQIFYESPYRVKDTLTNLLACYGDRQVVLVRELTKLFEEYQRGQISEVLTYLADHP
                     LKGECLLIVAGANQADASEPAEDLDLLALVQQAIAAGMKPNQAIKTVAKDYKVNRQELY
                     QQFHGLG"
     gene            complement(6571..6894)
                     /db_xref="GeneID:83705469"
                     /gene="yabA"
                     /locus_tag="I2432_RS08280"
                     /old_locus_tag="LJFMMFNO_01654"
     CDS             complement(6571..6894)
                     /codon_start=1
                     /db_xref="GeneID:83705469"
                     /gene="yabA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /locus_tag="I2432_RS08280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_01654"
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="WP_012680029.1"
                     /transl_table=11
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQGLVEENTILRLEN
                     TKLRERLSQLEHENLAKTSSKQGKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRELL
                     DRK"
     gene            complement(6887..7687)
                     /db_xref="GeneID:83705470"
                     /gene="ricT"
                     /locus_tag="I2432_RS08285"
                     /old_locus_tag="LJFMMFNO_01655"
     CDS             complement(6887..7687)
                     /codon_start=1
                     /db_xref="GeneID:83705470"
                     /gene="ricT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677460.1"
                     /locus_tag="I2432_RS08285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_01655"
                     /product="regulatory iron-sulfur-containing complex subunit
                     RicT"
                     /protein_id="WP_206158726.1"
                     /transl_table=11
                     /translation="MISTIGIKYCDTCSVRYVQTDQAFPVQTKLVISDGKGSRLAMVNS
                     PIKSFCKDDLPKDMPSVLRPANDMDYKAYQQNLQLAVQSKQKVAEMIDRNGLDMTLVDI
                     VFSLERRYVLITFTAQERVDFRQLLKDFAAYFKTRIELRQINSREEARVYGGLGPCGRP
                     LCCSSFLGEFPPVSIKMLKHQGLSLHSAKSLGCCGRLLCCLQYEDDFYQESKKKFPDYG
                     TIVRTAQGPGEVAGIDLFTDTIQVRFKDQLALVTYALEEVKVSE"
     gene            complement(7684..8559)
                     /db_xref="GeneID:83705471"
                     /locus_tag="I2432_RS08290"
                     /old_locus_tag="LJFMMFNO_01656"
     CDS             complement(7684..8559)
                     /EC_number="2.7.7.7"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83705471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516197.1"
                     /locus_tag="I2432_RS08290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_01656"
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="WP_111678453.1"
                     /transl_table=11
                     /translation="MDLAQKAPRVYQAFQEILQKKRLNHAYLFSGDFANFDMALFLAKT
                     LFCEQKRGNAPCHHCRACQLIEHNDFSDVTILEPVGQLIKTDTVKEMMTNFSQTGYEGR
                     RQVFIIKDCDKMHVNAANSILKYIEEPQGDCYMFLLTNDDNQVLPTIKSRTQVFHFPKD
                     EHYLSELAQKKGLLKHQADLLARLAANAQQLTAFSENTRMLELMAQAERFVSIWFQDKE
                     QAYLELNRLVQLAEEKEEQDMALTLLTLILAKDYSRQNAFTYLEAVYQARLMWQSNVSF
                     QNTLEYMVIS"
     gene            complement(8573..9208)
                     /db_xref="GeneID:83705472"
                     /gene="tmk"
                     /locus_tag="I2432_RS08295"
                     /old_locus_tag="LJFMMFNO_01657"
     CDS             complement(8573..9208)
                     /EC_number="2.7.4.9"
                     /GO_function="GO:0004798 - thymidylate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83705472"
                     /gene="tmk"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677458.1"
                     /locus_tag="I2432_RS08295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_01657"
                     /product="dTMP kinase"
                     /protein_id="WP_111678859.1"
                     /transl_table=11
                     /translation="MTRGRLITFEGPDGAGKTTVLERLVPLLQAALGQTIVTTREPGGV
                     AIAEKIRQLILDVSHTTMDDKTELLLYIAARRQHLVEKIVPALEAGHLVLVDRFIDSSV
                     AYQGAGRGLDKQAIQWLNHFATDGVDPDLTLYFDVPSELGLARIAQNTEREVNRLDLEQ
                     LDLHQRVRKGYLELALENPQRIVKIDASQDLESVVAAALAAIITHSQA"
     gene            complement(9523..9807)
                     /db_xref="GeneID:83705473"
                     /locus_tag="I2432_RS08300"
                     /old_locus_tag="LJFMMFNO_01658"
     CDS             complement(9523..9807)
                     /codon_start=1
                     /db_xref="GeneID:83705473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677457.1"
                     /locus_tag="I2432_RS08300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_01658"
                     /product="YlbG family protein"
                     /protein_id="WP_043053379.1"
                     /transl_table=11
                     /translation="MFQKQQRIGLVIYLYYNRDARKLMKYGDLYYHSKRSRYLIIYINK
                     EDMEEKLKDISRLKFVKEVKASAFDDIDCDFVGNLHREPLEIQAQPDQD"
     misc_feature    complement(9831..9844)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 21.45"
                     /tool="antismash"
ORIGIN
        1 atcaaagcca ggcttattga acttagctgc taagtgtgct tctgccatag ccataccaac
       61 tgcattggca attccttgtc ctaaaggccc tgtggttgcc tcaacaccat ctgtgtgatt
      121 gacctctggg tgacctggtg ttttagagcc ccattgacgg aagttcttca gatcctcaac
      181 agacacatca tagcctgcta ggtgcaatag gctgtaaagc atggcactac catgacctgc
      241 tgataggata aagcggtctc tatttgacca acggcggctt gtctttggat tgactgtcat
      301 aaaatggttc catagcacat aagccattgg cgctgccccc atcggcaaac ctgggtgccc
      361 agaatttgct gcttgaatag cgtccattga tagagtacga acagtattga ctgccagctg
      421 atcaattgca tcaaatgtca taagtaaaac ctctctgtgt attacaacta aaaattaaac
      481 gttttccatt gtctattgta acataaaagt aaactgattt ttaacaaaat cctataaagt
      541 ccttaaattc ttatccagcc acaagcattg ctgttcctag ctttttggga ttgacagctg
      601 tcctagccag ggcacctatg ctcataagaa aaagcaagcc tagctgttgc cagctaagca
      661 agcattcctc tttggtacct ccctttggct acagtaagcg ccagctgcct gttaattcta
      721 gcgcaacagc aaagcaccag ctgatcctgc cacctacaaa tagctggttc caaactcact
      781 gctatagcca cattagaaca taaggagagc tctatgctct tagcctattg cttaaaggaa
      841 gcccaatcct ccataaaggt ctttaagcca tttgttgtca atgggtgctc agtcatcttg
      901 gcaaacaatg gatctggaat ggttgcaatg tgtgccccta gctgtgcaac agcttcaaca
      961 tgagcagctg tccgaatgct agctgcgatg atttctgctt ggaagccata caaatcaatg
     1021 atcccacgca agtcactaat gagctgataa gggtctgcac caatatcctc taagcgcccg
     1081 ataaatggac tgatataggt cgcaccagcc ttcatcgcca ttagtccctg actaactgta
     1141 aaaataagcg tcacattcgt tttgatgcct tcttgtgata agacattagt tgcttttaag
     1201 ccctctgtcg tcatcggaat tttcacgacc acattgtcat gccacttggc aatgctccta
     1261 gcctctgcaa tcatttcctc tgcggttaat ccagttacct cggcactgat cgggccatca
     1321 acaatctcac agatttcttt gataactgtc tcaaaatcac gaccctcacg tgaaataatg
     1381 ctaggatttg tggtaacacc gtccacaaca ccaagctcat taatcgcacg aattgcctct
     1441 acatttgctg tgtctaagaa aaatttcatc ttaacctccg ttaaattagt ctgttaccag
     1501 tcattgaaca gcacgacgct gtttaccgtc tacactatca ttataaaagt cactcctagg
     1561 atataaaaga ccaagtatca cacagtctag tgttcaaaac tagacctagc taggggactc
     1621 tgtttcctta aaaggcctga ttcttacagc tcctctgtga aaaactgtgt ggctttgaca
     1681 gctcttttcc agccttgata tagcttttct cgacgggagt tactcatgct tgctttaaat
     1741 agctgacctg tggcatttaa ttgctttagc gtccccatgt cctcccagta gcctacagca
     1801 agcccggcca aaaaggctgc tcctagagct gtggtttcaa gattttcagc acgagcaata
     1861 tcaatcccta aaatatctgc ctgaaactgc atgagcatgt tattcatggc cgctccacca
     1921 tcaacacgta attgctggat ggtgatccct gaatccacct gcatggtatc aatcacatct
     1981 ctgacctgat aagcaataga ctgtagggta gccttaacaa aatcctcttt tgttgttcca
     2041 cgcgttaagc caaataccga gccgcgtgca ttggaatccc aataaggagc gccaagacct
     2101 gtaaaggctg gtacaacata cacctcatca gcattggttg aagccaaggc tagctgttca
     2161 gactcaaaag aggtttttat cattcgcagg ccatctcgca gccattgcac agcagagccg
     2221 gcaataaaga tagacccttc taaggcataa taaaccttgc cattaatacc atatccaatc
     2281 gttgttagca gattgctgct ggataattga gcctcttgac cagtattcat gatgataaaa
     2341 gagcctgtgc cataggtatt ttttaccata ccaggctcaa aggccagctg cccaaatagg
     2401 gcggcctgct gatcacccgc catgccagca atagctacct ctcctccgta aaaatgaaaa
     2461 ggagccgttt taccataaat ttccgaatta gacctgacct caggaagcat ggccttaggg
     2521 atattcagca aggccaatat ctcatcgtcc cattttagct ctttgatatt gtatagcatg
     2581 gtacgtgctg cattagaata atccgtcaca tgtaccgccc cgtccgttag cttccaaacc
     2641 agccaggtgt caatcgtgcc aaataataat tctccccttt cagctcgctc ttgagccccc
     2701 ggaacatgat caagaagcca tctgagcttg gtcgcagaaa aataagcatc tataaccaga
     2761 cctgtttttt catggatcat tttggtatag ccgtcacgct ttagctgctc tgcaatggag
     2821 gctgtttgtc tagactgcca cacaatagca ttatagatgg gcagacctgt ctgtttatcc
     2881 cagactaccg ttgtttcacg ctggttggta atgcctatag cttctatttg ctctggcttt
     2941 ataccagact ctataaaggc gtctgcaata acagactgca ccgaattcca aatgtgattg
     3001 gcgttatgct caacccagcc agcctgtgga aaaaactgtg gaaattcctt ttgactacta
     3061 accagcgctt ctcctttctt attgaaaata atggcacgag agctcgttgt gccttgatca
     3121 atagccataa tataggtttc agttcccatg tgtcctccat tctattgctg ctgagataag
     3181 acctctcaat catagcaagc gctttctatt ctattataca acatttacag cccatagcct
     3241 atccttatag tcataaaagt atcactctac caaatcctaa gctgataatg cctatcattt
     3301 caactaaaca gcccttatca cagccaaata ccagcaacaa aggcgggcta tagactaagc
     3361 gcctaaatgg ctgccaacaa gctccagctt tatcagctaa caaggagcaa caaaaaacct
     3421 aagccctttt agcatttaag ggcttaggag tgggctttta tcgcataagc ggtgatagct
     3481 tcttaatcgt aaacaggctc agaggacttt tctaaaatat ctccactatt agcgtcaata
     3541 gtatagctgt actcctgatc cttggcgtca aactcaatat cgtaaactaa cctaccatcg
     3601 tctgtttctt tcgtcagctt gagcctttga gcgtcagctg ctgtgattga ggcgtcctta
     3661 aaagcaagct ccttggcctt gtcctctgaa atgccagctg ctttatcagc ctgaccacca
     3721 gctgcttgag ctgaagcagg agcctcagtg attggctcag aggatttttc taggatctca
     3781 ccggtattag cgtcaatggt atagctgtac tcctgatcct tagcgtcaaa ctcaatatca
     3841 tagaccactc taccatcgtc tgtttctttc gtcagcttga gcctttgggc gtcagctgct
     3901 gtgattgagg cgtccttaaa ggcaagctcc ttggccttgt cctctgaaac acttgccttc
     3961 ctatgattct gagcgctgcc attttgacct agagcctgat tctgttgacg acaagctgat
     4021 aggctgatta aggatagact cgtaagagct attactgaaa gttttttgat tatcattgta
     4081 ttgaccttcc atctttattc atcttatttc ctctagtata cattaaataa tctgatttta
     4141 caattttaaa gcactaaaac tctcttaacc aggcttactc ccttatatga taccccccaa
     4201 agagcccata aggtaaaggt aatggctttg ctatccctca accaagaata gatcacccag
     4261 ccgaataaaa aaagcaatta ttggtagaat gagaagaatt attttggtct tatttattgc
     4321 cttcaccagc tatcaatcac tatttaatga ccattttggt atgaaccctc actgccactc
     4381 actatctgac accaagctac ttttaagtac cagcatcgat gtctcttaca cctccagtgc
     4441 ctatccatag ccgaccttca gcatgccagc atttctgatt aattacaaat actccctaaa
     4501 ctgttgaaaa agtatctaaa cgaatcatac aaaacttaat ttaccttttt tctatataaa
     4561 tagctaaaaa acatagtaag gtcacaaggg atataagatt tacattgctt atagtagtcg
     4621 ataagaaata aaaaattaaa aaaataaagt aggaagaccc ttctttgccc ctcttagcac
     4681 tgtcaaccag tatatagata aaaattaaag cataaagacc tgaaaaggta atcaataaca
     4741 tctaatttgc cttatatgcc aagtatgctc ctccagcagt aaagccggct atacatactc
     4801 cttttagcac cggatgaggt attttgcaaa acttaattcc tgtcttgaga cctcctaatc
     4861 ccccgataac agcattacct gctgcatttc ctcctgaaat gtcctgcatt tcatttattg
     4921 ctaatgctct ataatgttcc atttctatta tcctcctaaa acaaggttcc aaactcagaa
     4981 tctttctgtt tttctcattt ctaaaagcct cctgtgacac cagtagccca agtccagagt
     5041 gtaactccag tttttgcacc aatagatcca caagctactc cacatactgg aaatccaagt
     5101 gctgcgcctg cacttcctgc tccaatcaca cctgacacat tggcagccca attatttttt
     5161 tctctttcaa agctagcaag catttctgta gccattacgt caaatgattg tattgattgc
     5221 aaatactccc taaaaattga tgttgtccag ttgcaacctt gacatattta gtataaccct
     5281 tttttgtaaa acattcctcc atatcctaaa cagtcgttga aacgtcctaa atgggagcat
     5341 ttttttcgtt taagactgaa aagtaaccat taagggaggt cataaagcgg caagcctgtg
     5401 gctttagaaa ccttaaaaat gttaaaagca agattttcat tgctattaag agcatcaaag
     5461 aaagcaccag tctggtcctc actaggtgcg agttgtcagt caacccacta cagttgacgt
     5521 tgaggcaaaa aaagagaact gactagctct cttagtgtgc agacaaatct atctgcttac
     5581 attaatgaca attctcagaa gtacaaaaga cagctactag cccttgcttg agtacctctc
     5641 ctttacttag cctattagag attctctcag ccaagtgact ggtctaggtt aaaaccagcc
     5701 tagccaagcc catgaaattg ctgatagagc tcttggcgat tcaccttata atctttggcg
     5761 actgtcttga tcgcctgatt aggcttcatg ccagcagcaa tagcctgttg aacaagtgct
     5821 agcagatcca aatcctcagc aggctctgat gcgtcagctt gattggcacc tgccacaatc
     5881 aatagacact cacccttgag tggatggtct gctagatagg tcaagacctc tgaaatctga
     5941 ccacgctggt attcctcaaa aagcttggtt agctctctca ccagcaccac ctggcgatcc
     6001 ccatagcagg ccagcaggtt tgttagcgta tccttaacac gataaggaga ttcataaaag
     6061 atttgagtct ccggatagtc tgtcttagtc atcaaaaatg ccctttgctg cccagctttt
     6121 cttggcaaaa agccataaaa cagatgaggc tggggcacca agccgctagc aatgagaccg
     6181 gtaatgcccg cagaagcacc cggcaacgca acaatagtaa tgtctcgatc aatagctgct
     6241 ctaactagct catgaccagg atctgaaata gagggcatac cagcatcgga gacctgcgct
     6301 aatgaccttc cagccaacaa caggtctagc aaatctggaa ttttatcata agcattatgc
     6361 tcatgaaaac ttacctgctt tgtctttatt ccaaaatgct tgagcaacag accagtattt
     6421 ctcgtatcct ctgcacagat aatatccacc tcttttagaa cattaaccgc tctaaaggtc
     6481 atatcctcta ggttaccaat aggggtaggc accagataga gaacccctga ctctttttct
     6541 tccttgaagc tcttttggat ttgcatgact ctactttcta tctaatagct ctcgacaaaa
     6601 catacactct tcatcatttt cacgacgttg cccatagaaa aaattgcaga tatgaaagcc
     6661 ctcatcataa atgccctcca agtggtcctt gccctgctta gaagaggtct tggccagatt
     6721 ttcgtgctcc aattgactca agcgctcacg taatttggtg ttttcaagcc taaggatagt
     6781 attttcctct acgagtcctt ggacctgctt tttcattgct tcaatttcag ctaaagtcac
     6841 catcaggttt tgtgaaaagc catcaaaagc gtcaaacagt tcttttttat tcactaactt
     6901 taacctcctc taaagcatag gttaccaagg ccagctggtc cttaaagcga acctgaatgg
     6961 tatctgtaaa aagatcaatt cctgcaacct cacctggtcc ctgcgctgtt cttactatag
     7021 taccataatc aggaaatttt ttcttacttt cttgataaaa atcgtcctca tattgtaggc
     7081 agcacagcaa gcgaccgcag cagcccagag actttgccga atgcaaggac agcccttgat
     7141 gttttagcat tttgattgag actggaggaa actctcctaa aaagcttgaa cagcatagtg
     7201 ggcgaccaca gggacccagc cctccataaa cccttgcctc ctcacgacta ttaatctgac
     7261 gcagctcaat ccttgtctta aaataggcag caaaatcctt gaggagctgg cgaaaatcaa
     7321 cacgctcctg agcggtaaag gtaatcaaaa cataacgtct ctcaagggag aaaacaatgt
     7381 caaccaaggt catgtctaag ccatttctgt caatcatctc tgctactttt tgctttgact
     7441 ggactgctag ctgaaggttt tgctggtaag ccttgtagtc catgtcattg gcaggtctca
     7501 aaacagaggg catatccttt ggcaggtcgt ccttacaaaa gctttttatc ggagaattga
     7561 ccattgccaa tcgactgccc ttgccatcag atatgacaag cttggtttgc acaggaaagg
     7621 cctgatcggt ttgaacatac ctgacagagc aggtgtcaca atatttaatt ccaatcgtac
     7681 ttatcatgaa atcaccatat attctaacgt attttgaaag ctgacattgc tttgccacat
     7741 gagtctagcc tgataaacag cctccagata ggtaaaagca ttttgtcgac tgtaatcctt
     7801 cgccaagatt aaggttaaaa gagttagtgc catgtcctgc tcctcttttt cctctgctag
     7861 ctgaaccaag cgattaagtt ccagataagc ctgttcctta tcctgaaacc agatggaaac
     7921 aaaacgctca gcctgcgcca tcagctccaa catcctggtg ttttcagaaa aagcggtaag
     7981 ctgctgggca ttggctgcta atctggctaa gaggtctgct tgatgtttca aaagcccctt
     8041 tttttgggcc agctcagaga ggtagtgctc atcttttgga aagtgaaaaa cctgtgtacg
     8101 gctcttgatc gttggtagga cctgattgtc atcattcgtt agtaaaaaca tatagcaatc
     8161 cccttgtggc tcttctatat attttaaaat gctgttagct gcattgacat gcatcttatc
     8221 acaatcctta atgataaaaa cctgccgcct gccctcatag ccggtctgcg aaaaatttgt
     8281 catcatttct ttgacggtat cagtcttgat aagctgacca acaggctcta agatggtaac
     8341 atctgaaaag tcattgtgct caatcagctg acaagcacga cagtgatgac aaggagcgtt
     8401 gcctcttttt tgttcgcaaa aaagagtctt ggctaaaaaa agagccatgt caaaattggc
     8461 aaaatcacct gaaaagaggt aggcatgatt tagacgtttt ttttgcaaaa tctcttgaaa
     8521 ggcctgatag accctaggag ccttttgagc caaatccatt cctaacctcc tgctatgctt
     8581 gactgtgtgt gataatggca gctaaagccg ctgccacaac gctttctaag tcctgtgatg
     8641 catcaatctt gacaatacgc tggggatttt ccaaagctag ctccagatag cccttacgaa
     8701 cacgctggtg caggtctaac tgctcaagat caagacgatt gacctctcgc tcagtatttt
     8761 gtgcaatcct tgccaatcca agctctgagg gtacatcaaa atacagggtc aaatcagggt
     8821 caacaccatc tgttgcaaaa tgattgagcc attgaatggc ttgtttatcc agccctcttc
     8881 cggcaccctg ataagcaact gagctatcaa taaaacgatc cactaaaacc aggtgtcctg
     8941 cctcaagagc tggcacgatc ttttcaacca aatgctgcct tcttgctgca atatagagca
     9001 gcaactctgt tttatcgtcc atggtcgtgt gactgacatc taagatcagc tgacgaatct
     9061 tctcagcaat cgcaacacct ccaggctctc tcgttgttac aatggtttgt cccagagctg
     9121 cctgtaagag tggcaccagt ctttccagca ccgttgtttt tcccgctccg tctggacctt
     9181 caaacgttat taatcttcct ctagtcatct tatttccttt gtttatcgtt acctctattt
     9241 taccaaaatt tgcctgaaaa agcactgcca attcattgag cagtgccaga tcagaaaaaa
     9301 acctttttgt gctgcctttt agacgatagg gataaaaagc cacctctcaa agcaggcaat
     9361 ctgaggctgt gtctcagacc tcaaagccta tgccacgatt tgacaaatga caggcgtctc
     9421 agcccttatt ttctttatct aagcaagcca gcaaggctga tcaatcatgc tttaggcgat
     9481 tgatgagcta cgacatgcta tgcaagacag gagcaggcct acttagtcct gatcaggctg
     9541 ggcttgaatc tctagaggct cacgatgtaa attcccaaca aaatcgcaat caatgtcatc
     9601 aaaggctgat gccttcacct ctttgacaaa ttttagtcga ctaatgtcct ttagcttttc
     9661 ttccatgtcc tctttattga tgtaaataat aaggtatcga gaacgcttgg aatggtagta
     9721 caggtcacca tatttcatca attttctagc gtctcgattg taatacaagt agataaccag
     9781 cccaattctc tgctgttttt gaaacattta ggtactcttt tctttaatat tttgatacgg
     9841 tccaagctca tgtcgaggtc tatttgtcgc caagggaagc cctgtgtcga caagaatatc
     9901 ctcagacact gcctctgcaa tagctgtcga aatcgtcgcc aaaacctcct gcaaatcaac
     9961 ctgtgccaag cgcagctcag caaccaatgg gtgcagatca agctggcgct tttgatgaag
    10021 taactggcaa cgcaaagccc tagcatctgg acaagactca agcaggccct gcttaagctc
    10081 ataatcccgc ttaagctctt caaataaggc tagctcggtc tgtagggcca aatctgcctc
    10141 aaaagccttt ttagctaagg cataggtctg atactggctg cttgtcctaa tatcc
//
