LOCUS       NZ_CP065054            12154 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Streptococcus equi subsp. zooepidemicus strain SEZ13 chromosome,
            complete genome.
ACCESSION   NZ_CP065054
VERSION     NZ_CP065054.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus equi subsp. zooepidemicus
  ORGANISM  Streptococcus equi subsp. zooepidemicus
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2150055)
  AUTHORS   Borlee,G.I., Lakin,S.M., Kapuscinski,M.L., Abdo,Z., Stenglein,M.D.,
            McCue,P.M. and Borlee,B.R.
  TITLE     Complete genomes of eight Streptococcus equi subsp. zooepidemicus
            strains isolated from mares in estrus with endometriti
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2150055)
  AUTHORS   Borlee,G.I., Lakin,S.M., Kapuscinski,M.L., Abdo,Z., Stenglein,M.D.,
            McCue,P.M. and Borlee,B.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2021) Microbiology, Immunology, and Pathology,
            Colorado State University, 2025 Campus Delivery, Fort Collins, CO
            80523, USA
  REMARK    Annotation added by submitter
REFERENCE   3  (bases 1 to 2150055)
  AUTHORS   Borlee,G.I., Lakin,S.M., Kapuscinski,M.L., Abdo,Z., Stenglein,M.D.,
            McCue,P.M. and Borlee,B.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2020) Microbiology, Immunology, and Pathology,
            Colorado State University, 2025 Campus Delivery, Fort Collins, CO
            80523, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Coverage                   :: 246
            Sequencing Technology :: Illumina NextSeq;Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015689395.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 02:47:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 1,970
            Genes (coding)                    :: 1,933
            CDSs (with protein)               :: 1,933
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 16 of 37
            Pseudo Genes (incomplete)         :: 20 of 37
            Pseudo Genes (internal stop)      :: 16 of 37
            Pseudo Genes (multiple problems)  :: 14 of 37
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:11:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 571828
            Orig. end                         :: 583982
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065054.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12154
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12154
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12154
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7154](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7154
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            206..2209
                     /db_xref="GeneID:83704379"
                     /gene="metG"
                     /locus_tag="I2432_RS02795"
                     /old_locus_tag="LJFMMFNO_00560"
     CDS             206..2209
                     /EC_number="6.1.1.10"
                     /GO_function="GO:0000049 - tRNA binding [Evidence IEA]"
                     /GO_function="GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83704379"
                     /gene="metG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006062.1"
                     /locus_tag="I2432_RS02795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00560"
                     /product="methionine--tRNA ligase"
                     /protein_id="WP_043030544.1"
                     /transl_table=11
                     /translation="MTKKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGYDV
                     FYLTGLDEHGQKIQDKAQEAGLSPQAYVDGMAEEVKALWKLLDISYDKFIRTTDDYHEE
                     VVAAVFERLLAQDDIYLGTYSGWYSVSDEEFFTESQLAEVYRDEAGKVIGGVAPSGHEV
                     EWVSEESYFLRLSKYADRLVAFFKAQPDFIQPDGRMNEMLKNFIEPGLEDLAVSRTTFT
                     WGVPVPSDPKHVVYVWIDALLNYATALGYGQKDHANFDKFWSGRVFHMVGKDILRFHSI
                     YWPILLMMLDLPMPERLIAHGWFVMKDGKMSKSKGNVIYPEMLVERFGLDPLRYYLMRS
                     LPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGRVPAYVKNVTAFDADL
                     EQVVADHISEYHKQMEAVDYPRALEAVWTIISRTNKYIDETAPWVLAKAADSKEQLASV
                     MAHLAASLRVVAHLIQPFMMTTSAAMMEQLGLGAKYHLEELDLADLPTGVTVVAKGEPI
                     FPRLDMEAEINYIKEQMKMSTAKPQEADWDPEKVALKSEKDTITFEAFDAIELRVAEVK
                     EVSRVEGSEKLLRFRLDAGDGEDRQILSGIAAYYPNEQELVGKKVQIVANLKPRKMMKK
                     YISQGMLLSAEHDGKLTVLTVDPSVPNGAIIG"
     misc_feature    2701..2714
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.6"
                     /tool="antismash"
     gene            2767..3660
                     /db_xref="GeneID:83704380"
                     /locus_tag="I2432_RS02800"
                     /old_locus_tag="LJFMMFNO_00561"
     CDS             2767..3660
                     /codon_start=1
                     /db_xref="GeneID:83704380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515165.1"
                     /locus_tag="I2432_RS02800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00561"
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_024273139.1"
                     /transl_table=11
                     /translation="MGDIKIEIGKRIRTARLDKGFTQIDICDDESELTIRQLARIENGQ
                     AMITLPKIIFLSEKLGIPVQDLIDEKIEIPKRYLELKNKLIKSHTYGDKERIRHHESMF
                     NEIYEEFYDNLPEEEQLLVEVLQVRLDVFSSRNSEFGISLLEEYFHQILKKKYHSYNDL
                     LIIDLYLFCCAVGLEDKTYFDDLSQKVLSYIDYSDFERIYVLEKILLSILIQVEPEKYL
                     LYTKVFREIMETTNNFQHKPAIYAFEAKYYLQVRKDVEKTIELYDKSIMFARMLNDSVL
                     VSNLEKEKLEDLGNLT"
     gene            4013..4153
                     /db_xref="GeneID:83704381"
                     /locus_tag="I2432_RS02805"
                     /old_locus_tag="LJFMMFNO_00563"
     CDS             4013..4153
                     /codon_start=1
                     /db_xref="GeneID:83704381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515166.1"
                     /locus_tag="I2432_RS02805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00563"
                     /product="hypothetical protein"
                     /protein_id="WP_012515166.1"
                     /transl_table=11
                     /translation="MEQFKNLSQSELQNTNGGGAWSPIVTVPVPVVLPMKVVKWVAGFF
                     E"
     gene            4266..4424
                     /db_xref="GeneID:83704382"
                     /locus_tag="I2432_RS02810"
                     /old_locus_tag="LJFMMFNO_00564"
     CDS             4266..4424
                     /codon_start=1
                     /db_xref="GeneID:83704382"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2432_RS02810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LJFMMFNO_00564"
                     /product="hypothetical protein"
                     /protein_id="WP_021320463.1"
                     /transl_table=11
                     /translation="MTRFETLDNMNFVSLSEAEMMEMTGGGFDIAAPIKAIFNAGKEFG
                     RALAKWI"
     gene            4438..4584
                     /db_xref="GeneID:83704383"
                     /locus_tag="I2432_RS02815"
                     /old_locus_tag="LJFMMFNO_00565"
     CDS             4438..4584
                     /codon_start=1
                     /db_xref="GeneID:83704383"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2432_RS02815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LJFMMFNO_00565"
                     /product="hypothetical protein"
                     /protein_id="WP_021320464.1"
                     /transl_table=11
                     /translation="MEQLKNLTLYHLIILIVVILLLWYFAPSIINAIFEIGKEFGHALA
                     KWF"
     misc_feature    4470..4483
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 23.01"
                     /tool="antismash"
     gene            4607..4774
                     /db_xref="GeneID:83704384"
                     /locus_tag="I2432_RS02820"
                     /old_locus_tag="LJFMMFNO_00566"
     CDS             4607..4774
                     /codon_start=1
                     /db_xref="GeneID:83704384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622446.1"
                     /locus_tag="I2432_RS02820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00566"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_012515168.1"
                     /transl_table=11
                     /translation="MENFVNLSEQELKEMTGGAVLETAAVIYVTKWVAGGAIALFGAGA
                     FVGYTEGKKK"
     misc_feature    4617..4633
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.66"
                     /tool="antismash"
     gene            5001..7154
                     /db_xref="GeneID:83704385"
                     /gene="comA"
                     /locus_tag="I2432_RS02825"
                     /old_locus_tag="LJFMMFNO_00567"
     CDS             5001..7154
                     /codon_start=1
                     /db_xref="GeneID:83704385"
                     /gene="comA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672141.1"
                     /locus_tag="I2432_RS02825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00567"
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="WP_012515169.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.6e-41, bitscore:
                     137.0, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1132.0,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFRRKHYRAQVDTRDCGVASLAMIYGYYGSYYSLATLREAAKTT
                     QEGTTAFGLVKVSEALGFETRAIRADMSLFDVEGLSYPFIAHVLKKGKFLHYYVVTGQD
                     KKYIHIADPDPTVKLTCLSRQRFASEWTGVTIFIAPTPEYKPHKEKKNSLLDFVPLLAK
                     QKGLITNIVVATLLVTIINIIGSYYLQSIIDTYVPSVMKNTLGIISVGLVIVYALQQVI
                     SYAQEYLLLILGQRLSIDVILSYIKHIFNLPMSFFATRRTGEIVSRFNDANSIIDALAS
                     TILSIFLDVSVIVIISLVLFSQNVNLFVISLLALPIYAVIIFAFMKPFERMNQETMEAN
                     SIMSSSIIEDINGIETIKSLTSEKIRYQKIDKEFVAYLKKSFNYSRAESQQRALKKVAQ
                     LILNVSILWIGANLVMDGKMSLGQLITYNSLLIYFTNPLENIINLQTKLQTAQVANNRL
                     NEVYLVESEFSEKKNVQDLSLTSGDIKFNDVSYRYGYGKDVLSEINLHIKRGSKVAFVG
                     ISGSGKTTLAKMIVSFFEPSKGEISLNNVNLNQIDKHALRQHINYLPQQPYVFNGTILE
                     NLLLGAREGTTQEDIMKAVELAEIKSDIERMPLNYQTELTSDGAGISGGQRQRIALARA
                     LLTDSPVLILDEATSNLDILTEKRIVDNLMALDKTLIFIAHRLTIAERTEHVVVLDKGK
                     IIEQGTHRDLVSQNGFYAHLVNS"
     gene            7169..8536
                     /db_xref="GeneID:83704386"
                     /locus_tag="I2432_RS02830"
                     /old_locus_tag="LJFMMFNO_00568"
     CDS             7169..8536
                     /codon_start=1
                     /db_xref="GeneID:83704386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515170.1"
                     /locus_tag="I2432_RS02830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00568"
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_206156376.1"
                     /transl_table=11
                     /translation="MNNNILESAEFYNRRYHNFSSRIILPTLLLFLFGVFFLAFAKKEI
                     SIISTATVEPNIILSNIQSTSNNTILTNNLKDNKYVKFGDLLIKYDSRKEGIQQETYQT
                     QLNNLQIQKEQLELLKASIEAGNSQFPEKDNYGYYQTFIDYLNQINTLSANVNQQNENV
                     SSQNTAASNQQVEIENAIKGLTSQISDYQSVRSAIQNGTVVDLDNRAYSIYRSYLTQTS
                     SLVDNTDKTAVINQFVAQIDSQINQLESSVAGYRIQHAGSGVQQSYSSTLESQLASLKA
                     QSITKVEQELSALSNQITELEGTNKLQKNTSSKTSIVANEDGVLHLNQEFIGANIVPSG
                     TIFAQVYPEMKKGTVVKITGYLPSTEISSISIGDTARFSTKDDNGETLDLIVKLSSIDN
                     AATKTKNGNYFKVESKLQLKEKEAQLLKYGLEGKFILITGTKSYLNYYIDKFIGEK"
     gene            9045..9281
                     /db_xref="GeneID:83704387"
                     /locus_tag="I2432_RS02835"
                     /old_locus_tag="LJFMMFNO_00569"
     CDS             9045..9281
                     /codon_start=1
                     /db_xref="GeneID:83704387"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2432_RS02835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LJFMMFNO_00569"
                     /product="hypothetical protein"
                     /protein_id="WP_024273149.1"
                     /transl_table=11
                     /translation="MINDIRNVRREVKLTRKANKILEDMLRVAPSDSFSEFIRKRLLNN
                     FPEEKYIENFLENYLKIELLYGIRNISRDIHKI"
     misc_feature    complement(9474..9490)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 25.4"
                     /tool="antismash"
     gene            9739..10752
                     /db_xref="GeneID:83704388"
                     /gene="nrdF"
                     /locus_tag="I2432_RS02840"
                     /old_locus_tag="LJFMMFNO_00570"
     CDS             9739..10752
                     /EC_number="1.17.4.1"
                     /GO_component="GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA]"
                     /GO_function="GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /GO_function="GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83704388"
                     /gene="nrdF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /locus_tag="I2432_RS02840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00570"
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="WP_012515172.1"
                     /transl_table=11
                     /translation="MTQYYYDRSQSPIDYALSQTKKSLRSVNWNQLNDDKDLEVWNRVT
                     QNFWLPEKVPVSNDLNSWRSLGEDWQRLITRTYTGLTLLDTVQATVGDVAQIQHSLTDH
                     EQVIYTNFAFMVAIHARSYGTIFSTLCSSQQIEEAHDWVVSTESLQERARVLIPYYTGD
                     DPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSGYKYQ
                     QKVAALSPEKQAEMKAFVFDLLYQLIDLEKAYLRELYDGFDLADDAIRFSVYNAGKFLQ
                     NLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYVMGVTEETTDDDWEF"
     gene            10754..11161
                     /db_xref="GeneID:83704389"
                     /gene="nrdI"
                     /locus_tag="I2432_RS02845"
                     /old_locus_tag="LJFMMFNO_00571"
     CDS             10754..11161
                     /GO_process="GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83704389"
                     /gene="nrdI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678368.1"
                     /locus_tag="I2432_RS02845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00571"
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="WP_206156919.1"
                     /transl_table=11
                     /translation="MAARIMLVYFSSRSNNTHRFVQKLDAKALRIPISGEPLLVNEDYI
                     LIVPTYAAGGSDSKGAVPKQVIHFLNKANNRRHCKGVISSGNTNFGDTFAIAGPIIAQK
                     LQVPLLHQFELLGTQRDVIKVQAILAGDEAL"
     misc_feature    complement(11328..11343)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 20.67"
                     /tool="antismash"
ORIGIN
        1 tatcctcctc ttatagtttt tggtgactgt ttgtttttta ctattgacta tcaaggggag
       61 gcagcggtta atagggtaag tgatcatttt ttatgctaaa attgtggtat aatgtcagtt
      121 gatagctgac aggactcagc ttgggaatgg gtattgccct gatccctgaa ttagaaaggt
      181 ttcattagcc ttgaaaggac gtaagatgac taagaaaaaa ccattttata ttacaactcc
      241 tatttactac ccgtcaggta agcttcatat tggctccgcc tatactacga ttgcctgtga
      301 tgttcttgct cgctataagc gtatgatggg ttatgatgtt ttttatttga ctggtcttga
      361 tgagcatggt caaaagattc aggataaggc acaagaagcc ggcttatccc ctcaagctta
      421 tgttgatggc atggctgagg aagtcaaggc actatggaag ctccttgata tctcttatga
      481 taagtttatc cgcaccactg acgactatca tgaggaggtg gttgctgctg tatttgagag
      541 gttgctggcg caggatgata tttatttagg gacctattca ggctggtatt ctgtttcaga
      601 tgaggaattt tttacagaaa gtcagttagc tgaggtttat cgcgatgaag ctggcaaggt
      661 cattggtggt gtggcaccat cagggcatga ggtcgaatgg gtctctgaag aatcttattt
      721 tttacgcctg agcaagtatg cggatagatt ggttgccttc tttaaggctc agcctgattt
      781 tattcagccg gacggtcgca tgaatgaaat gcttaaaaac ttcattgagc caggtctgga
      841 ggatctagcg gtgagtcgaa caacctttac ctggggagtt cctgtaccat cagatcctaa
      901 acacgttgtt tatgtctgga tcgatgctct cttaaactat gcaacagcac taggctacgg
      961 tcagaaggat catgctaact tcgataagtt ctggagtggt agggttttcc atatggttgg
     1021 taaggatatt ttgcgtttcc acagcattta ttggccaatt ttgttgatga tgcttgattt
     1081 accaatgcca gagcgtttga ttgctcatgg ctggtttgtc atgaaggatg gtaaaatgtc
     1141 taaatcaaag ggaaatgtga tttaccctga aatgctggtt gagcgttttg gtcttgatcc
     1201 tctgcgttat tacctcatgc gttccttgcc ggttggctct gatggcacct ttaccccaga
     1261 ggattatgtt ggtcggatta attatgagct ggctaatgac cttggtaacc ttttgaaccg
     1321 cacagttgcc atgattaata aatactttga cggcagggtt cctgcttatg tcaaaaacgt
     1381 tacagccttt gatgcggatc tggagcaggt tgtggctgat catatttccg aataccacaa
     1441 gcaaatggag gctgttgatt acccacgtgc cttagaggcc gtatggacga ttatttcacg
     1501 cacgaacaaa tacattgacg agacagctcc ttgggtgctt gccaaagcag ctgatagtaa
     1561 ggagcagcta gccagtgtga tggcacattt agctgcaagt ctgcgcgtgg tggctcattt
     1621 gattcagcca tttatgatga caacctcagc tgctatgatg gagcagctag gtctcggtgc
     1681 caaatatcat ctggaagagc ttgatttagc agatcttccg acaggtgtga cggttgttgc
     1741 caaaggagag ccgatctttc ctcgtcttga tatggaagca gagattaact atatcaaaga
     1801 gcaaatgaaa atgagtacag ctaagccgca agaagcagac tgggatcctg aaaaggtagc
     1861 ccttaagtct gaaaaggata cgattacttt tgaggccttc gatgctatag agcttcgtgt
     1921 agcagaggta aaagaggtat cacgagttga aggctctgaa aaactgctgc gctttagatt
     1981 agatgctggt gatggcgaag accgtcaaat cctgtcaggt attgcagcct attacccaaa
     2041 tgaacaagag ctcgtcggca agaaggtgca aattgttgcc aacctcaagc cgcgaaaaat
     2101 gatgaaaaaa tatatcagtc agggcatgct tttgtcagca gagcatgatg gcaagctgac
     2161 agtgctgacg gttgacccga gtgtgccaaa tggtgcgatt attggctgat tgggctttta
     2221 gatgacaata tcccgtatgg gtcttgctaa tagattgatt agcaggagca tacgggattt
     2281 ttggtgtctt aaaaacgaaa taaacaacaa tgctttagac agaagtgctc tttcaaaaat
     2341 ggtatataat gattaaaaag aaagaaatgg atatgtgaat caagtgacgt ttttgactga
     2401 tatctcttta aactgctact gcgccacagc cttgtatgac gatgctagtg ttaacgtgtg
     2461 aacgaacaat gaatcagatt ggctagttta ttttgaaaga tgctcaacaa agctattaga
     2521 gttatttgag gtgtcgcctt gattttattt actttattac cttctgagag gaagatgcta
     2581 agggagatgg catctaagtc tcaaatccgt atgtcggatt cattatttgt ggcttccaat
     2641 ttcacccaga agacctatga agagagtagt gaaagtaggt aattactata tcatttcatt
     2701 attttttaaa aataaaaaat tttgttataa tttagaatat atgaaagtat aggagaattt
     2761 attttagtgg gagatattaa gattgagatt ggcaaaagga ttcgtacagc acgcttagat
     2821 aaaggattca ctcagataga tatttgtgat gatgaatctg aattgacaat acgacagttg
     2881 gcgcgtattg agaatggaca agctatgatt acattaccta agataatctt tctgtcagag
     2941 aagcttggta ttccagttca ggacttaatt gatgaaaaaa tagagattcc taaacgctat
     3001 ttggaattga aaaataaact cataaaatct catacttatg gcgataaaga gagaattcgt
     3061 caccatgagt caatgttcaa tgaaatttat gaagagtttt atgataattt accagaagag
     3121 gaacagctat tagtagaggt tttacaagtt agattagatg ttttttcgag tagaaattct
     3181 gagtttggaa tcagtttact tgaggaatat tttcatcaga ttctaaaaaa gaagtatcat
     3241 agctataatg accttttgat tattgactta tatctctttt gttgtgcggt tggtttggag
     3301 gataaaactt attttgatga tttatcacaa aaagttctgt cgtacattga ctatagtgat
     3361 tttgagcgca tatatgtatt agaaaaaata ttgctttcta tactgataca agtcgaacca
     3421 gagaagtatc ttttgtatac gaaagtcttt agagaaatta tggaaacaac gaataatttt
     3481 cagcataagc cggctatcta tgcttttgaa gcaaagtatt atttacaagt gcggaaagat
     3541 gttgaaaaga cgatagagtt atacgataag tcgattatgt tcgcaagaat gcttaatgat
     3601 tcagtattag taagtaatct agaaaaagaa aaactagagg acttaggcaa cctgacttag
     3661 aagtcatgtt gcttttttta tgttttgaat ataataagat tataaaaaac gagacaaatt
     3721 aaaaagaaag gagcaaaggt atgattctag caattatact tacagctttg atttggattg
     3781 caggatgata caatcatcta tttgttatca aatcaaaaaa atttttatcg agcataaaga
     3841 gattaccata aaaaataact ctgactttga taggttatgt agggttgcta tcaatcatga
     3901 actgttcaag attatatatt taactcttag agcagaagag gtaatcaaat atacaggtgg
     3961 agtaatccga catatagaag tttcacgtat agaaattgaa gaaggagaat agatggaaca
     4021 atttaaaaat ttaagtcaat ctgaattaca aaacaccaat ggaggaggtg cttggtctcc
     4081 aattgtgaca gtaccggtac cagttgtttt acctatgaag gtagttaaat gggtagctgg
     4141 tttctttgaa tagcatatta aactaaaaat aaaaggagga agctataaaa gatattcgag
     4201 tctcaaatag ataatagaga tactgtaaca cacagataaa atttaaatat aaggagaaaa
     4261 ataatatgac tagatttgaa acactagaca acatgaattt tgtatcatta tctgaagcag
     4321 aaatgatgga aatgactgga ggtggctttg atattgcagc tcctatcaaa gctattttta
     4381 acgctggtaa agaatttggc cgtgcattgg caaagtggat ttaaagggta gaaaattatg
     4441 gagcaactaa aaaatttaac gttatatcat ttaattattt taattgtagt tatcttgtta
     4501 ctatggtatt ttgctccaag tattataaat gccatttttg aaataggtaa agaatttgga
     4561 catgcattag caaaatggtt ttaacataat aaaagggaga aaaagtatgg aaaattttgt
     4621 aaatttatca gaacaagagt tgaaggaaat gactggaggt gcggtattgg agactgctgc
     4681 agttatttat gtcacaaaat gggtagcggg tggtgcgatt gccctttttg gagcaggagc
     4741 ttttgttgga tacactgaag ggaagaaaaa ataatttcta agtacgaaac aattgcaact
     4801 atcgtgttta gagtctataa acgtactata tttcaaaagt taggagcaaa atctaatgct
     4861 caaataagaa gagcttggga caagccaccc caagctcttc ttatttgagc tgaaactaat
     4921 tttgtctaga attattttgg cgattttcta aatatataaa attataatag aaaataaaat
     4981 ataacaattt aggagattct atgaaattta gaagaaaaca ctaccgtgct caggttgata
     5041 ctcgcgattg tggcgtagct tctctggcca tgatttacgg ttattatgga tcgtattatt
     5101 cgctagcaac gttacgtgaa gctgcaaaga caacgcagga agggactaca gcttttggtt
     5161 tagtcaaagt ttctgaggcg cttgggtttg aaacgagagc tattagggct gacatgagtt
     5221 tatttgatgt ggaaggatta tcttatcctt ttattgccca tgttctaaaa aaaggtaagt
     5281 tccttcatta ttatgtggta acaggacaag ataaaaaata tattcatatt gcagatccag
     5341 atccaactgt aaagttgact tgtctttctc gtcagcgatt tgcttcagag tggacaggtg
     5401 tgacaatttt tatcgctcct actcctgaat acaaacctca caaggaaaag aaaaatagtc
     5461 tgctagattt tgtgcctctc ctagcaaagc aaaaaggatt gattactaat atcgttgtgg
     5521 caacgctttt ggtaacgatt atcaatatta ttggttcgta ttatcttcaa tcaatcattg
     5581 atacctatgt tcctagtgtg atgaagaata cactaggtat tatttccgta ggtttggtta
     5641 ttgtctatgc ccttcaacaa gtgatctcat atgcgcaaga gtatttattg ctgatattag
     5701 ggcaacgttt atctattgat gtaattcttt cttatatcaa acatattttc aaccttccta
     5761 tgtctttttt cgcgacgaga aggaccgggg aaattgtttc tcgttttaat gatgctaatt
     5821 caatcattga tgctttagca agtacaattt tatctatttt ccttgatgtt tctgtcattg
     5881 taattatatc tcttgttctt ttttcgcaga atgtaaatct atttgttatc agtttgcttg
     5941 ctctgcctat ctatgcagtg attatctttg ccttcatgaa gccatttgaa cggatgaatc
     6001 aagagacaat ggaagcaaat tcaatcatgt catcttctat tattgaagat ataaacggta
     6061 ttgagactat taaatcatta actagcgaaa agataagata ccaaaaaata gataaagaat
     6121 ttgtagctta tcttaagaaa tctttcaact atagtagggc agaaagccaa caacgagcat
     6181 tgaaaaaagt agctcagctt atcctaaacg tgagcatact ctggatagga gccaatctgg
     6241 ttatggacgg aaaaatgagt ttaggtcaac tgattaccta taactctctc ctaatctact
     6301 ttacaaatcc tctggagaat attatcaatc ttcaaacgaa acttcaaacc gctcaagtcg
     6361 ctaataatcg attgaatgag gtatatctag ttgaatccga gttctctgaa aagaagaatg
     6421 ttcaagactt atctttgacc tctggtgaca taaaattcaa tgatgttagc tatcgatatg
     6481 gatatggaaa agatgtttta tcagaaataa atttgcatat taaacgagga agcaaggttg
     6541 cttttgttgg gatttcaggt tctggaaaaa cgactttagc taagatgatt gtcagtttct
     6601 ttgaaccaag caaaggcgaa atatcattaa ataatgtgaa tctaaatcaa attgacaaac
     6661 atgctcttcg ccagcacatt aactatcttc ctcaacaacc ttacgtattt aatgggacaa
     6721 tccttgagaa tttacttctt ggagcacgtg aagggacgac tcaagaagac attatgaagg
     6781 cagtagaatt ggctgaaatc aagtctgata ttgaacgtat gcctttgaat tatcaaacag
     6841 agttaacctc agatggtgca gggatttctg gaggtcaacg tcaacgtatt gccttagcaa
     6901 gggcattatt gacagattct cctgttttga tattagacga agctacaagt aacttggata
     6961 ttttgactga gaagcgaatt gttgataacc tgatggcttt agataaaacc ttgatattta
     7021 ttgctcaccg tttaactatt gcagagcgta cagagcatgt cgtcgttttg gataaaggaa
     7081 agatcataga gcaaggaaca cacagagacc ttgtttctca gaatggcttt tatgctcact
     7141 tggtcaatag ttaggaggat ttgataaaat gaataacaat atattagaaa gtgcggaatt
     7201 ttataaccgt cgttaccaca acttttctag tcgtatcatt ctacctactt tgctgctttt
     7261 cttgtttggt gttttcttcc tagcatttgc aaagaaagaa atttctatta tttcaactgc
     7321 aacagttgaa ccaaatataa ttctttccaa tattcaatct acaagtaata atacaatatt
     7381 aaccaataat ctaaaagata ataaatatgt caaatttgga gacttattga taaaatatga
     7441 ttcaagaaaa gaaggtattc aacaagagac ctatcaaact caattaaata atttacaaat
     7501 ccaaaaagaa cagttagagc tattaaaagc aagtattgaa gctggaaaca gtcaatttcc
     7561 agaaaaagat aattatggat attatcaaac atttattgac tacttaaatc agataaacac
     7621 cttatcagcc aatgtaaatc agcaaaatga aaatgtctct tcacaaaata ctgcagctag
     7681 caatcagcaa gtagaaattg agaatgccat caaaggtctc acaagtcaaa tttcagatta
     7741 tcaatctgtt cgctcggcga ttcaaaatgg aacagtggta gatcttgata atcgggctta
     7801 ttctatctat aggagttatc taactcaaac atcgagtttg gtagataata ctgataaaac
     7861 cgctgttatt aatcaatttg ttgctcaaat tgatagtcag atcaatcaac tagagagtag
     7921 tgtagctggt tatcggattc agcatgctgg ttcaggagtg caacaatctt actcttcaac
     7981 tctagagagt caactggctt ccttaaaagc gcaaagcata accaaagttg agcaagagtt
     8041 gtctgctttg tcgaatcaaa ttacagaatt agagggaaca aataaacttc agaagaacac
     8101 atcatctaag acctctatag ttgccaatga agatggtgtg ttgcatctta atcaggaatt
     8161 tattggtgca aatattgttc cttcaggaac aatatttgct caagtttatc cagaaatgaa
     8221 aaaaggaact gttgtaaaaa tcacaggtta tttaccttcg acggaaatat caagtatttc
     8281 tattggagat acagcacgat tttccacgaa agatgataat ggcgaaactc ttgatttaat
     8341 agttaaatta tctagtattg acaatgctgc aactaaaaca aaaaatggga attattttaa
     8401 agttgaatct aagttacagc ttaaagaaaa agaagctcaa ttattgaaat atgggcttga
     8461 gggtaaattt attttaatta cgggaacaaa aagttacttg aattactata ttgataaatt
     8521 tatcggtgag aagtaaactg gatcatcgtt ttaatatcag gaagctatct taagttttta
     8581 gtaatgaaga atatttatct actctaggtt ctgagggaca ctgaaatagt ggaattattt
     8641 ctgggattta tcacgctaaa aatattttat gattacaaga aatacacatc aactttttga
     8701 ccgactatta ttgtcggtct tttttatgaa tacaacagtt acctcactag aaaagtttta
     8761 aagccatata aagaactact ttcgtttgtt ttttgtgata gaaaaaataa gaataattgt
     8821 ttaaactatt tttaggaggg atagagtctc caagctttta atattatcag tcaaagtgaa
     8881 ataacaattt caaagaggct gatgacatta tttttgaggt attgtgacca caatacctga
     8941 gctcaccaca acccaaagat caagtttagg ttcattaatt cctagtcata tagtgtacgg
     9001 cgtacagttg tttattgtac gactaggaat catgaagagt tatcgtgata aatgacataa
     9061 gaaatgttag acgagaagta aaacttactc gtaaagcaaa taagattttg gaagatatgc
     9121 tgagagttgc cccatcagat agtttttcag agtttattcg taaaagactt ttaaataatt
     9181 ttcctgaaga aaagtatatt gaaaactttc tagaaaatta tttaaaaatc gagttgttgt
     9241 atgggattcg gaacatcagt agagatattc ataaaatcta attttacgct actcaaggac
     9301 agtaagcagc agctttgctg atgcttaatc tggatatgag ctgtctgcta gttgagggga
     9361 ttggtgagca tacagacagg gctttcgtta aggtgctgct agctcctttt atgatttctt
     9421 tttcataaat taatctatat attgtatcaa aaaatattgc aaagcactat atattgtgtt
     9481 attataacaa acataagaat taagtatact taattcagtc ggagaaaaaa gcgggcttag
     9541 ccttgcttac aaagcagttg ctgactggtt agggatcttc agcaagaaat tatcattagc
     9601 ctgtaggatt gccctttaga gcaagtcttt ttgtgttgcc taaatgtggc acttagcagt
     9661 cttccctagt cctaaagctg tagaaggctt ggcagctgaa ggattgatat tgcgactatc
     9721 aagcttgaag gagaaaacat gacccaatat tattacgacc gatcacagtc acccattgac
     9781 tatgccttgt ctcaaaccaa aaaaagtttg cgctctgtca attggaatca gcttaatgac
     9841 gacaaggacc tggaggtctg gaaccgagtg acgcaaaatt tttggctgcc tgagaaggtt
     9901 cctgtttcta atgaccttaa ttcttggcgt tctttggggg aggattggca aaggttaatt
     9961 acaagaacct ataccggctt gaccttgcta gatacggttc aagcaacagt tggagatgtg
    10021 gcacaaattc aacattcttt gaccgatcat gagcaggtga tttatactaa ttttgccttt
    10081 atggttgcta ttcacgctag gtcctatggg acaatctttt cgaccctgtg cagcagtcag
    10141 cagatcgaag aggcccatga ttgggtggtg tcaacagaga gcttacagga gcgagcgcgt
    10201 gtgttgattc cgtattatac tggtgatgac cctctgaagt ctaaggtcgc agcagctatg
    10261 atgccaggat ttttgctgta tggcggcttt taccttcctt tttacctgtc agctagaggc
    10321 aaaatgccca atacctcaga tattatccga ctaatcctga gagataaggt cattcacaat
    10381 tattacagtg gctacaagta tcaacaaaag gtggcagccc taagtcctga aaagcaggct
    10441 gagatgaagg cctttgtgtt tgatctgctt tatcagctga ttgatttgga gaaggcttac
    10501 ctgagggaat tgtatgacgg ctttgatctt gctgacgatg cgattcgctt tagcgtctat
    10561 aatgctggaa aatttttgca aaatctaggg tatgattctc cctttactga ggaggaaaca
    10621 cgtatttctc ctgaggtatt tgctcagcta tcagcgcgag cagatgagaa ccatgacttt
    10681 ttctcaggaa atggctcttc ttacgtgatg ggagttactg aggaaaccac tgatgacgat
    10741 tgggagtttt aagatggctg caaggataat gcttgtttat ttttcatcgc gatctaacaa
    10801 cactcaccgc tttgtgcaaa agctagatgc aaaggccctc aggattccaa ttagtgggga
    10861 gcctctcttg gttaatgagg actatattct tattgtcccc acctatgctg ctggtggctc
    10921 agacagcaag ggagctgttc ccaagcaggt gattcatttt ctaaacaagg ctaacaatcg
    10981 aaggcactgt aagggggtta tttcctctgg caataccaac tttggtgata cctttgccat
    11041 tgcaggaccc attattgccc aaaaattgca ggtgccgctt ttgcaccaat ttgaattgct
    11101 ggggacacaa agagatgtca tcaaggtgca ggcgatttta gcaggtgatg aagccttata
    11161 actaatgata gaaaaacgat cacacattgt aacgaaagga aaaaagaatg tcacaaaaag
    11221 agtcctacct ctcattaaat gcccttacac gctttaaacg agcagatggt acctatcact
    11281 ttgacagtga taaggaggct gttcagcgct atttagagga gcatgtctac ccacaaagcc
    11341 tatcctttga tagtttggag gagaaatggc gttatttggt ggcgcatgat tattatgatc
    11401 aagagatttt aaaggcttat tcggctgaat ttatcactga agcctttacc tacgcttacc
    11461 agcaaaagtt ctcatttttg agcttaatgg gagccatgaa attttaccag tcctatgcgc
    11521 ttaaaagcct agatgggtca caatacctag agcattttga ggatcgtgct gttatgaatg
    11581 ccttgttttt ggctaatggt gatcctgatt tggtgcggga tttgattgat cagatactta
    11641 gcaggcgctt tcaaccggca acccctacct ttttaaatgc aggtaaaaag cgtcgtggcg
    11701 aatacatttc ctgttatttg ctgcgaattg aagacaacat ggaatctatt tcgcgtgcga
    11761 ttggtacgag cctgcagctc tcaaaaagag gtggtggcgt tgctttgtgc ttaactaacc
    11821 taagagaggt tggctctccc ataaaaggca ttgaaaatca ggcgacaggc attgtgccgg
    11881 tgatgaagct gctggaggat tccttttcct atgccaatca gctagggcaa aggcaggggg
    11941 caggtgcggt ttacctcaat gtgcaccacc cagagattct gaccttttta gatacgaaga
    12001 gagaaaatgc agacgaaaaa atccgtatca agtcactggc gttaggcgtt gtcattcctg
    12061 atattacctt tcagctggcg cgggaaaata aggacatggc actattttct ccctatgaca
    12121 ttaagcgagc ttatggtaag gacatgtcag atat
//
