LOCUS       NZ_CP065054            10294 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Streptococcus equi subsp. zooepidemicus strain SEZ13 chromosome,
            complete genome.
ACCESSION   NZ_CP065054
VERSION     NZ_CP065054.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus equi subsp. zooepidemicus
  ORGANISM  Streptococcus equi subsp. zooepidemicus
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2150055)
  AUTHORS   Borlee,G.I., Lakin,S.M., Kapuscinski,M.L., Abdo,Z., Stenglein,M.D.,
            McCue,P.M. and Borlee,B.R.
  TITLE     Complete genomes of eight Streptococcus equi subsp. zooepidemicus
            strains isolated from mares in estrus with endometriti
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2150055)
  AUTHORS   Borlee,G.I., Lakin,S.M., Kapuscinski,M.L., Abdo,Z., Stenglein,M.D.,
            McCue,P.M. and Borlee,B.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2021) Microbiology, Immunology, and Pathology,
            Colorado State University, 2025 Campus Delivery, Fort Collins, CO
            80523, USA
  REMARK    Annotation added by submitter
REFERENCE   3  (bases 1 to 2150055)
  AUTHORS   Borlee,G.I., Lakin,S.M., Kapuscinski,M.L., Abdo,Z., Stenglein,M.D.,
            McCue,P.M. and Borlee,B.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2020) Microbiology, Immunology, and Pathology,
            Colorado State University, 2025 Campus Delivery, Fort Collins, CO
            80523, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Coverage                   :: 246
            Sequencing Technology :: Illumina NextSeq;Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015689395.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 02:47:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 1,970
            Genes (coding)                    :: 1,933
            CDSs (with protein)               :: 1,933
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 16 of 37
            Pseudo Genes (incomplete)         :: 20 of 37
            Pseudo Genes (internal stop)      :: 16 of 37
            Pseudo Genes (multiple problems)  :: 14 of 37
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:11:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 155032
            Orig. end                         :: 165326
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065054.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10294
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10294
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10294
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5294](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5294
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(179..197)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 23.84"
                     /tool="antismash"
     gene            191..481
                     /db_xref="GeneID:83704008"
                     /locus_tag="I2432_RS00895"
                     /old_locus_tag="LJFMMFNO_00177"
     CDS             191..481
                     /codon_start=1
                     /db_xref="GeneID:83704008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677258.1"
                     /locus_tag="I2432_RS00895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00177"
                     /product="hypothetical protein"
                     /protein_id="WP_206158912.1"
                     /transl_table=11
                     /translation="MLIPINEYYIEEEYAYALRLMRQMLERQRIATVARLVNSQGEAIK
                     KAFKDQIKATASQTKESLQIMEGQLDTAIKGAVRDQLEQVLKKKLPKYNKL"
     gene            780..1067
                     /db_xref="GeneID:83704009"
                     /gene="groES"
                     /locus_tag="I2432_RS00900"
                     /old_locus_tag="LJFMMFNO_00178"
     CDS             780..1067
                     /codon_start=1
                     /db_xref="GeneID:83704009"
                     /gene="groES"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514806.1"
                     /locus_tag="I2432_RS00900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00178"
                     /product="co-chaperone GroES"
                     /protein_id="WP_012514806.1"
                     /transl_table=11
                     /translation="MLKPLGDRVVLKFEAEKEQTVGGFVLAASHKEATKVGTVVAVSET
                     GIRTITGDIVPPSVAVGDKVLVEYGSGLEVKDGDQELVICREADILAVLA"
     gene            1135..2760
                     /db_xref="GeneID:83704010"
                     /gene="groL"
                     /locus_tag="I2432_RS00905"
                     /old_locus_tag="LJFMMFNO_00179"
     CDS             1135..2760
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83704010"
                     /gene="groL"
                     /gene_functions="other (smcogs) SMCOG1299: chaperonin
                     GroEL"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /locus_tag="I2432_RS00905"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES; misfolded
                     proteins enter the barrel where they are refolded when
                     GroES binds; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00179"
                     /product="chaperonin GroEL"
                     /protein_id="WP_206158913.1"
                     /transl_table=11
                     /translation="MAKDIKFSADARESMVRGVDILADTVKVTLGPKGRNVVLEKAFGS
                     PLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREGLK
                     NVTAGANPIGIRRGIEAATTTAVEALKAVAQPVSGKEAIAQVAAVSSRSEKVGDYISEA
                     MERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFILITD
                     KKISNIQDILPLLEEVLKTSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKAPGFGD
                     RRKAMLEDIAVLTGGTVITEDLGLELKDATMAALGQAAKVTVDKDNTVIVEGAGSSEAI
                     SNRVSLIKSQLETTTSEFDREKLQERLAKLAGGVAVIKVGAATETELKEMKLRIEDALN
                     ATRAAVEEGIVAGGGTALINVMDKVAALDLDGDAATGRNIVLRALEEPVRQIAYNAGYE
                     GSVIIDKLKNSAAGIGFNAATGEWVDMIATGIIDPVKVTRSALQNAASVAGLILTTEAV
                     VATKPEPAAPAMPQGMDPGMMGGF"
     gene            complement(3095..3565)
                     /db_xref="GeneID:83704011"
                     /locus_tag="I2432_RS00910"
                     /old_locus_tag="LJFMMFNO_00180"
     CDS             complement(3095..3565)
                     /codon_start=1
                     /db_xref="GeneID:83704011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920900.1"
                     /locus_tag="I2432_RS00910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00180"
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="WP_206158914.1"
                     /transl_table=11
                     /translation="MLEVIKNWVNICVKEGDKILLLNRQHDDFPGWIQPGGKVNISESF
                     FEAAVRELKEETGLTALNLQLKGISGFTNPDKPERYVYYDFLCEDFEGKLLTESREGLP
                     QWHAIADLDKLDMQEDIRQRLPLYWRQGSFERLHYWSDKEKKVVKTVTHLYD"
     gene            3801..4664
                     /db_xref="GeneID:83704012"
                     /locus_tag="I2432_RS00915"
                     /old_locus_tag="LJFMMFNO_00181"
     CDS             3801..4664
                     /codon_start=1
                     /db_xref="GeneID:83704012"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR01716"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622164.1"
                     /locus_tag="I2432_RS00915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00181"
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="WP_206158915.1"
                     /sec_met_domain="TIGR01716 (E-value: 4.3e-52, bitscore:
                     173.7, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNTDLMSLGELYKELRIARGLRLKDVARDNLSVSQLSKFENGQS
                     MLAADKLLLAISGIHMTFAEFGHAVNGYKVSDFFQLGTKVAELQTASDIDGLKELLSQY
                     KGDDIFSVYNRLNELIIKSAVYSLDPNVKIEESDKQLLTEYLYSIEEWTEYELYIFGGC
                     LAILSDEDMIFLGKAFIERDKIFLSLPQHRKAAHITLLNIIMTLLERRQLFYIDFFIKK
                     LEELIIYQDMFTIVSLNFFKKIHTYLSEESSHKSDIEDYINLVKQLGNPQFVQILHRTL
                     SVILDS"
     gene            5001..5294
                     /db_xref="GeneID:83704013"
                     /locus_tag="I2432_RS00920"
                     /old_locus_tag="LJFMMFNO_00182"
     CDS             5001..5294
                     /codon_start=1
                     /db_xref="GeneID:83704013"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514809.1"
                     /locus_tag="I2432_RS00920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00182"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_042669851.1"
                     /sec_met_domain="TIGR03651 (E-value: 5.4e-13, bitscore:
                     45.9, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATLERHHKMLLLSMALGAASWLALGLISLPDLAGALGISTSNAS
                     TTVNLLSAYSTVSSVIAIVGAITGVGSIGAGMTATVYTLSNTKERLLLFSGS"
     gene            5304..6443
                     /db_xref="GeneID:83704014"
                     /locus_tag="I2432_RS00925"
                     /old_locus_tag="LJFMMFNO_00183"
     CDS             5304..6443
                     /codon_start=1
                     /db_xref="GeneID:83704014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514810.1"
                     /locus_tag="I2432_RS00925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00183"
                     /product="hypothetical protein"
                     /protein_id="WP_206158916.1"
                     /transl_table=11
                     /translation="MKHVFKYHNGNILGYLNIVGFTLLFYGSVIGMIMFRNSLTYDGAG
                     SLISTFILVSGTYHSVKSTFPYEGKRHPTFILTPYYLLKHLLLSVNKVYALQIRVVSIG
                     VICLAIAFPEQLAWIALSLLAVFMYWLSLSLATIFQVAGRILNLLMIYGMYLHQESLVI
                     ACLCLNAVILVLNITIDFRYPYQWLAARAKKTENTITLLRLVVNMITDHLALMILFIIF
                     CIATTYFTQRLLLLFDYSSFSIPVFFLSATTYITLLEVMVGKDIKELELDRGLVVLHFL
                     NKDISVYNAYRNSQFLLSAHILAIIHTGCVIGLLPFLLNGQAALFLSNLVMIPFIYFIS
                     FCYFVKSIKMVADDLSVFRWVILVLYFILVVIAMVGSRL"
     gene            6440..6922
                     /db_xref="GeneID:83704015"
                     /locus_tag="I2432_RS00930"
                     /old_locus_tag="LJFMMFNO_00184"
     CDS             6440..6922
                     /codon_start=1
                     /db_xref="GeneID:83704015"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /locus_tag="I2432_RS00930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00184"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_111692112.1"
                     /transl_table=11
                     /translation="MTMRKFLRYYISFIIFSIVIGILIGLLITSDTVILSKPERGWDFT
                     LEVLKNNSNNFLSYIFLFFLSPALQLIDLVSVVIQITLGMRKSGFLITALGLFPHGLLE
                     IPNFLFYQGLSQYMLWTVLTEKSMTSFLERERRYVRYYLISYCVLLIAGIMEGLLG"
     gene            6919..7506
                     /db_xref="GeneID:83704016"
                     /locus_tag="I2432_RS00935"
                     /old_locus_tag="LJFMMFNO_00185"
     CDS             6919..7506
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83704016"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622167.1"
                     /locus_tag="I2432_RS00935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00185"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_012678903.1"
                     /transl_table=11
                     /translation="MKIVNESKSFSNRKILNHISFDFSKGLYKVEGNNGSGKTTFLRLL
                     AGLERFDSGHRLQHKGDILYLDTNAIGVVPLTIEDNLTLLWKTFNASPDEETLSVINQF
                     FGERLLDHYLTASVGTKAKVGLSLLFVKDWDYIFIDEALSTLDGFSLDMVAGRLLALKE
                     KATVFYVSHNLSHQQLLSESQVIYLNGKGEIY"
     gene            7503..7985
                     /db_xref="GeneID:83704017"
                     /locus_tag="I2432_RS00940"
                     /old_locus_tag="LJFMMFNO_00186"
     CDS             7503..7985
                     /codon_start=1
                     /db_xref="GeneID:83704017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678904.1"
                     /locus_tag="I2432_RS00940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00186"
                     /product="hypothetical protein"
                     /protein_id="WP_206158917.1"
                     /transl_table=11
                     /translation="MKGNPFNRNLSWLIAYVLLLSCYVFILVDGGFLKNSVQELEVLLG
                     QTPLAIKLFLMVISIAFSIISLLVEYFLSKFLVLLFLEAGIVSLNDVVIAKSVTLVIHL
                     VALLLKLSPSPLFQFVVNALGIVIMYGLSIKRQGNRTTALLFCLPFTLDILATLLL"
     gene            8010..8525
                     /db_xref="GeneID:83704018"
                     /locus_tag="I2432_RS00945"
                     /old_locus_tag="LJFMMFNO_00187"
     CDS             8010..8525
                     /codon_start=1
                     /db_xref="GeneID:83704018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622170.1"
                     /locus_tag="I2432_RS00945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00187"
                     /product="hypothetical protein"
                     /protein_id="WP_043983904.1"
                     /transl_table=11
                     /translation="MKKILYLSVLLGTSILATLIKIYNTFFAKLKASSGHSTGNIEIDA
                     LLSESRLVLERQHAFTTNGINKLFVMMMSLMLLLALYYLIKKDLKAVYSYLLYLVLTLV
                     HAIYSFFSLSPLARLYSDDMLRATIQTGEKAKLIFSVVLFLLYLLIVVANNRVKSNVEL
                     TSSVGIDV"
     misc_feature    9168..9181
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.52"
                     /tool="antismash"
     misc_feature    9169..9182
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.7"
                     /tool="antismash"
ORIGIN
        1 aagcgtttag acctatctgt caacgctgcg tcagattttt tcaaggaact ggatcgctta
       61 aaggatcaat ccaaggcaga gtttcacctc catcgggcag ctcttgaaga ggagcgagca
      121 cagtcaacca agacttttcg tcaagagcag gctcaaagag agacagagtt atcagatatg
      181 aggagagctt atgctaatac caataaatga gtactatatc gaagaagaat acgcctatgc
      241 cctgaggcta atgcgtcaaa tgctggagcg tcaaaggatt gccacagtcg ctaggcttgt
      301 aaacagccaa ggtgaagcga tcaaaaaggc gtttaaagac cagatcaaag cgactgccag
      361 ccaaaccaag gaaagccttc agatcatgga agggcagcta gatacggcta ttaaaggagc
      421 tgttagagat cagctggaac aggtattgaa gaagaagctg ccaaaataca acaagctata
      481 gtcaaatggt gtcacctcta agaaaccata gggggtcaat caatacccac caagcaagta
      541 ttatcaaaag aggttatcag ccgccagagc ttttgtcctg ttattttgaa aggtgatagt
      601 tgtatacagg gtgactgata gccaaagatg ggagaggacc agctgggtct tttctttttt
      661 cctagacttt ttttcttgac tatttttgac caagtgataa aatagagaag aaagttagca
      721 ctcataaaac aagagtgcta acaaaataga taatctagtc attattggag gtactattca
      781 tgttaaaacc gttaggagat cgtgtggtcc taaaatttga agcagaaaaa gagcagaccg
      841 tcggtggctt tgttcttgct gctagtcaca aagaagccac taaagtaggg acagttgtag
      901 ctgttagtga gacaggtatt cgtactatta ctggagacat tgttccacca agtgtggctg
      961 taggtgacaa ggttttggtt gaatatggat caggcttaga ggttaaagat ggcgatcaag
     1021 agcttgtcat ctgtcgtgaa gctgacattc ttgcagtttt agcttaattg tgaatcccta
     1081 tcatttcagg taggattaac agatatacat tcaaatagga atagaggaaa taagatggca
     1141 aaagatataa aattttcagc agatgctcgt gaaagcatgg tacgtggtgt tgatattttg
     1201 gcggatactg ttaaggtaac ccttggccct aaagggcgta atgtagtcct tgaaaaggcc
     1261 tttggttctc ctttgatcac aaatgatggt gtaacgattg ccaaggagat cgaattagaa
     1321 gatcattttg aaaatatggg ggctaagctt gtatcagagg tggcttcaaa gacaaatgat
     1381 attgccggtg acgggacaac aacagctact gttttgactc aggctattgt gcgtgaaggg
     1441 ctgaaaaatg taacagcagg tgctaaccca attggtattc gtcgtgggat tgaagcagcg
     1501 acaacgacag ctgttgaggc tttaaaggct gttgctcaac cagtatctgg aaaagaagcg
     1561 attgctcaag tagcggcggt gtcatcacgt tctgaaaagg ttggtgacta tatttctgaa
     1621 gctatggagc gcgtgggaaa tgatggtgtt atcactattg aggaatcacg tggtatggaa
     1681 acagagcttg aggttgttga gggaatgcag tttgaccgcg gctatttgtc acaatacatg
     1741 gttactgaca atgaaaaaat ggttgctgat cttgaaaacc catttatcct gattactgat
     1801 aagaaaattt ctaatatcca ggatattctt cctttgcttg aggaggtgtt gaaaacaagc
     1861 cgtccattgc tgatcattgc tgatgatgtt gatggcgaag cccttccgac cttagtgctt
     1921 aataaaattc gtggtacctt caatgttgta gcggttaaag cgcctggctt tggagatcgt
     1981 cgaaaagcga tgcttgaaga cattgctgtc ttaactggtg gaacagttat cactgaggat
     2041 cttggtcttg aattgaagga cgctacaatg gctgccctcg gacaagcggc taaagtaact
     2101 gttgacaagg ataatacagt gattgtagaa ggtgcaggca gctcagaggc tatttcaaac
     2161 cgcgttagcc tgatcaaatc acaattagaa acaaccacat ctgaatttga tcgtgaaaaa
     2221 ttacaagagc gcttagcaaa attagcaggt ggtgttgcag ttatcaaggt gggtgcagct
     2281 actgaaaccg agctaaaaga aatgaagctt cgtattgagg atgcgctaaa tgcaacacgc
     2341 gctgcagttg aagaaggcat cgttgcaggt ggtggtactg ctcttatcaa tgtcatggat
     2401 aaggttgcag cccttgatct tgatggtgat gcagcaactg gtcgcaatat cgtgcttcgt
     2461 gcgcttgaag agcctgtgcg tcaaattgct tacaacgctg gctatgaagg ttcagtgatt
     2521 attgataagc tgaaaaatag tgctgctggt attggtttta atgcagcaac tggtgaatgg
     2581 gttgatatga ttgctacagg tatcattgac ccagtcaagg taacacgctc agcacttcaa
     2641 aatgcagcct ctgttgcagg tttgatccta accactgaag ctgttgttgc tactaagcca
     2701 gagcctgctg caccagcaat gccacaagga atggacccag gcatgatggg tggcttctaa
     2761 caaaaagcct gttaggatag gctagttgtt ctatcatagg atatcaaaat aattgataaa
     2821 gactcccctg acgttaaggt gatatgatta tccctaaaac gcccagggga gcttttttat
     2881 tgcttacctt agcacgctta acggacatgt gacttgtctt tgttttaaat cattgacaac
     2941 aattaaaatg accagtgtga attagtgctt ttctgaagac aaaaaagcaa aagccctgag
     3001 aaatcaaggc ttttgcagga gatttatgaa aaagaaaaat ttaggataat gtaagtataa
     3061 gctagtttcc ttaaggaaag cttaactgaa gcaattaatc gtagagatgg gtgactgttt
     3121 tgacgacttt tttctccttg tcagaccaat aatgaagacg ttcaaaagat ccttgtcgcc
     3181 agtagagcgg tagcctttgt cgaatatcct cttgcatgtc tagcttatct agatcagcaa
     3241 tagcatgcca ttgtggtagg ccctctcgtg attcagttag cagcttgcct tcaaaatctt
     3301 cgcaaagaaa gtcataataa acatagcgct caggcttatc aggattggta aagcctgaga
     3361 ttccctttaa ttgcaggttt aaggcggtta agccggtctc ctcctttagc tcacgcactg
     3421 ccgcttcaaa aaaggattct gagatgttaa ccttgccacc cggttgaatc cagccaggaa
     3481 agtcgtcatg ctggcggttg aggagtagga tcttgtcgcc ctccttgaca cagatattga
     3541 cccaattttt gatgacttca agcattagct acctcgtttt tagttttatt atatcaaaaa
     3601 aagatcagta tgaatagctg agagatttat tgatatggtc ataatggctt cagatagttt
     3661 tactgctgct ttagccaaag cttgattaag atctgtataa ataacataaa aatactggtt
     3721 cacatcatta tttaatagat ttattaccgt attaatggta taattatgta aatattagaa
     3781 ctataggcag agagaatgta atgaataata cagatttgat gagcttggga gagctctata
     3841 aggagcttcg tattgctcgt ggacttaggc taaaggacgt tgctagagac aatttatcag
     3901 tttcacagct atctaagttt gaaaatggcc agagcatgct ggcagctgac aaactactat
     3961 tagctatttc aggaatccat atgacatttg ctgaatttgg tcatgctgta aatgggtata
     4021 aagtcagcga tttttttcag ttaggcacta aggttgccga gctacaaaca gcatcagata
     4081 tagatggatt gaaggagctt ctgagccaat ataagggtga tgacattttt tctgtttata
     4141 atagattaaa tgaattaatc attaaaagtg ctgtctactc acttgatcca aatgttaaaa
     4201 ttgaagagtc agacaagcaa ttattgacgg aataccttta tagtattgaa gagtggacag
     4261 aatatgagct atacattttt ggtggctgtt tggccatttt gtctgatgag gatatgattt
     4321 ttttgggaaa agcatttatc gaacgagata agattttcct ttctcttcca caacatagaa
     4381 aggcagctca catcactcta ttaaacatta tcatgacttt actggaaaga aggcagcttt
     4441 tttatattga cttttttatc aaaaagctag aggagttgat tatctatcaa gatatgttca
     4501 ctatagtgag cttaaatttt ttcaagaaaa tacacactta tttaagtgaa gaaagcagtc
     4561 ataagagtga tattgaggat tatattaacc ttgttaaaca gcttggcaac cctcagtttg
     4621 tccaaattct acatcggacg ctatcagtga ttctagatag ctaatactaa aaaaagaggc
     4681 ccaatcgttt ggtagttaga gcggccaaag ccaccaaatg aataaaaggg ttggtgagaa
     4741 aataaaaact tttcgcataa tgtctaacct ccttggtaag gatattatac agaggggaag
     4801 cctcaatctc agcaattcgc aaatatgaaa atattaaaaa aagtatcatc atatttgcga
     4861 atatcgagaa tatgatgata gtcgtgatat aactactgtg ttaatgacct agctaagtat
     4921 taataagtct aaatataagc tacagaaagg tcgctcctta tactagtttg agggctaatg
     4981 gtctggttag gaggaagcgt atggcgacat tagaacgaca tcataaaatg ttattattgt
     5041 caatggcatt aggggcagca agctggttag cactagggct cattagcttg ccggatcttg
     5101 caggtgcttt aggaataagt acttctaatg ctagtactac agttaattta ttgagtgctt
     5161 acagtacagt ttctagcgtt attgcaatag ttggtgctat tacgggcgta ggatctattg
     5221 gagctggaat gacagctact gtttatacct tatcaaacac aaaggaaagg ttattgctat
     5281 tttctggcag ttaatgatct aatatgaaac atgtttttaa ataccataac ggtaacattt
     5341 tagggtatct caatattgtt gggtttaccc ttttgtttta tggctcagtg ataggtatga
     5401 taatgtttag gaattctttg acctatgatg gagctggttc tctaatatcg acctttattc
     5461 tggtatcagg aacctatcat tctgtgaaat caacatttcc atatgagggg aagcgtcatc
     5521 caacgtttat actaacaccc tattatctac ttaagcatct attgctttca gttaataagg
     5581 tttatgcttt gcaaattcga gtggttagca taggagtcat ctgtttagct atagcctttc
     5641 cggagcagct tgcttggata gccttgtctt tgctggcagt atttatgtat tggttatcgc
     5701 tttcactagc gactattttt caagtagctg gaagaatcct gaatcttcta atgatttatg
     5761 gcatgtacct ccatcaggag tcgctggtca ttgcttgtct ttgtttaaat gcggttattc
     5821 ttgtgcttaa tattactatt gatttccgtt atccctacca atggctggca gcaagagcta
     5881 agaagacgga aaacacaatt actctattaa ggcttgttgt caatatgata acagatcatt
     5941 tagctttaat gattctgttt atcatcttct gtatagctac gacctatttc acgcaaaggc
     6001 tattgttgct gtttgattat tctagcttta gcataccagt ttttttctta tctgctacta
     6061 cctatatcac gttactagag gttatggtag gtaaagacat taaggagctt gagttagacc
     6121 gaggcttggt tgttttacat tttttgaata aggatatcag tgtttacaac gcttatcgta
     6181 acagtcaatt cttgctttct gctcacatct tagctattat tcatacggga tgtgtcatag
     6241 gcttactacc atttttacta aatggtcaag ctgctctttt tctatctaac ctagtgatga
     6301 tccccttcat atactttata tcattttgct atttcgtaaa atcaataaaa atggttgctg
     6361 atgatttgtc agtgtttagg tgggttattt tggttttgta ttttattcta gtggtcattg
     6421 caatggtagg tagtcgttta tgacaatgag gaaattttta aggtactata tcagttttat
     6481 tattttctca atagtgattg ggattttaat tggtttactg attaccagtg atactgtcat
     6541 attatctaag ccagagcggg gctgggattt tactctggag gttttaaaga ataatagtaa
     6601 taattttttg tcttatattt tcctcttctt tttgtcccca gccttgcaat tgattgattt
     6661 ggtatcggtt gttatacaaa ttactttagg aatgaggaag tctggatttc taataactgc
     6721 cttgggtttg tttccgcatg gtttgctgga gatccctaat tttctctttt atcaaggctt
     6781 gagtcaatat atgctatgga ctgttttgac tgaaaaaagc atgacgtcct tcttagaaag
     6841 agaaagaaga tatgtaaggt actatcttat atcgtattgt gttttgttaa ttgctggtat
     6901 tatggaaggg ttactcggat gaaaatagta aatgaaagca agtcattttc aaacagaaaa
     6961 atcttaaatc acatatcctt tgactttagt aaagggctgt ataaggttga agggaataat
     7021 ggtagtggta aaaccacttt tttgcgcttg ttagcaggct tagaacggtt tgatagcggc
     7081 catcgcttac agcataaggg agatattcta tatcttgata ctaatgctat cggtgtagtt
     7141 cccttaacta ttgaagacaa tctgacatta ttatggaaaa cctttaatgc ctctcctgat
     7201 gaggagacct tgtctgtgat taatcagttc tttggagaac ggttactaga tcattatctg
     7261 acggcttctg taggaacaaa ggctaaggtg ggcttatcct tgctttttgt aaaggattgg
     7321 gattatattt tcatagacga agccctatca acgcttgatg gctttagtct tgatatggtt
     7381 gcaggaagat tattagcatt aaaagaaaag gctactgttt tttacgtttc tcataatcta
     7441 tcacaccagc agctattgtc agaatcacaa gtgatctatc ttaatggaaa aggagaaatc
     7501 tattgaaagg taatcctttt aatcgtaatc tttcttggtt aatagcctat gtgctattac
     7561 tttcttgtta tgtattcata ttagtagatg gtggtttttt aaaaaacagc gttcaagaat
     7621 tggaagtcct gctcggccaa acaccactag ctataaagct gtttttgatg gtgatatcaa
     7681 tagcttttag catcatatct ttattggttg agtatttttt aagcaaattt ttagtgctgc
     7741 tttttcttga agctggcatc gtttctttaa atgatgttgt tattgctaaa tcagtgacgt
     7801 tagtgatcca tttagttgct ttgctattga agctatctcc ctctccactt tttcagtttg
     7861 tagtgaatgc tttagggatt gtgatcatgt atggattgag tatcaagaga caaggaaata
     7921 ggacaactgc tcttttgttt tgtttaccct ttacactgga tattctagca acattgttgc
     7981 tttagtgatt ttgagttgga ggaaaagtaa tgaaaaaaat cttatattta tcagtacttt
     8041 taggaacgtc aattctagca acattgatta aaatttataa cacttttttt gcaaagctaa
     8101 aggcttcatc aggtcattca actgggaata tagagattga tgccctttta tctgagtcta
     8161 gactagtgtt ggaaaggcag catgccttca caacgaacgg aatcaataag ctattcgtta
     8221 tgatgatgag tctaatgctg ttactagcct tatactatct gataaaaaag gatttgaagg
     8281 ctgtctatag ttatttgctt tacctggtgt taaccttggt tcatgcgatt tatagcttct
     8341 tttcgttaag tcccttggca aggctttatt ctgatgatat gctgagggct acaatccaga
     8401 cgggagagaa agctaaattg attttttcag tggttctttt tcttctttat ctgcttattg
     8461 tggtggccaa taatagagta aaatctaatg tagagctgac ttcatctgtt gggatagatg
     8521 tgtaatacct ttcacattgt gacctttaaa gccttttagc ttctctaatt ttcagagtaa
     8581 ctaggaggct gtttgtgacg ctcttagcgt tttttcgttt ctatcaaagt ttatctgcgt
     8641 taatgctatc tatttccgta gctgagatac aagatattat gatatgtgtt gtcataccat
     8701 aaaaaaacac cttacaagat atttgatgat caatcctcct tgaattaagc tttctttaag
     8761 caagtgtgtg tatactaact gtaatcctaa ataattggtt acctaatgat aactaatttt
     8821 ttcataacct ccttcctaaa aacgatgtca gggcagtcgg gattgattgc tgttatggca
     8881 tcgtttttgt tgcgctttga tagcaaatct agctgtatgt caaatagctt atcgggattg
     8941 atgaagatga tcagcagggg tctcttggct ctaggttatg ctgctgtttc atgagaaaca
     9001 gctcttgatg atcaatttag gtagtattag tggtggtcat cttagccttt taggctttga
     9061 gctgctttat gtttgctcat ttttaatgtg ttagtgttta cacaaataat tctcattttt
     9121 tgatataata gaaaaggtaa ggaagggtag gagataagga tgacaagatt ttttcaaaaa
     9181 aaacaggcca aaagcttagc aattggtttt gtgagcttgt ttgctgtgct cattgggtat
     9241 ggagcttttc actattctaa ggccagtcgt atcaatgcct atctacaagc aaggtcatca
     9301 ggctctgggc aggtgcttga aaatatcaag gaatacctcg tatgggctga taccaatgag
     9361 cagctgacca atgatcaagc aagatacacc agctttaaga gatatagtaa ggcagagcta
     9421 gtaagtaagc gtcatcagct gcaaacagca actgcagctg atgatatgta tgtcaaatcg
     9481 gttggtcgca aatttttgat tttccctgat tatcgcatag cagtgaagcc tgtggccctg
     9541 actatcaaga ccaatgtgcc aaatgttgat atcttgttga accataaaaa gcttgctgta
     9601 tcacagtcag aggattttag cgcaacgatt gaaaggcttc cggctgctga ttataaggcg
     9661 agtatcaatg gctattataa ggatcgtaag attaaggtta gcaagaccta tgatggcaag
     9721 aacaatacac tggatttgag ggttaccttt aagacattta cggtaaccag taatgtcaga
     9781 gacggcgagc tttatgttga tggtgagcgc gtggaaactt tgaaggatgg gcagcttcag
     9841 ctggaagact atcctgtgac agaaaccgca aaggcctata ttaaaaagac ctttcctgat
     9901 ggcgagctaa cctccagcaa gcatgccctt gctcctgttg ctgagggctc ccagctagag
     9961 attaacattg agcatctctt gactgaggaa caggcaggac aggtgttggt ggcagccttt
    10021 gatcaattgc ttacttatct caatcaagga caggatccag cgagcctttc aaccgttttt
    10081 gagcaggggg ctaataatgc tttttacaaa ggcttgaagg attccatcaa ggcaaaattt
    10141 caaactgatc ccagaaaggc ttctcgttta agcattccgt ctattttgtt aagcaagttg
    10201 actcaaattg gtaagcagtc ttatctagta gactttacag ctgtttatga ttttgtttac
    10261 gataaggaaa ctgatcctga aaaaggcacc tcag
//
