LOCUS       NZ_CP065054            10252 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Streptococcus equi subsp. zooepidemicus strain SEZ13 chromosome,
            complete genome.
ACCESSION   NZ_CP065054
VERSION     NZ_CP065054.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus equi subsp. zooepidemicus
  ORGANISM  Streptococcus equi subsp. zooepidemicus
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2150055)
  AUTHORS   Borlee,G.I., Lakin,S.M., Kapuscinski,M.L., Abdo,Z., Stenglein,M.D.,
            McCue,P.M. and Borlee,B.R.
  TITLE     Complete genomes of eight Streptococcus equi subsp. zooepidemicus
            strains isolated from mares in estrus with endometriti
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2150055)
  AUTHORS   Borlee,G.I., Lakin,S.M., Kapuscinski,M.L., Abdo,Z., Stenglein,M.D.,
            McCue,P.M. and Borlee,B.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2021) Microbiology, Immunology, and Pathology,
            Colorado State University, 2025 Campus Delivery, Fort Collins, CO
            80523, USA
  REMARK    Annotation added by submitter
REFERENCE   3  (bases 1 to 2150055)
  AUTHORS   Borlee,G.I., Lakin,S.M., Kapuscinski,M.L., Abdo,Z., Stenglein,M.D.,
            McCue,P.M. and Borlee,B.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2020) Microbiology, Immunology, and Pathology,
            Colorado State University, 2025 Campus Delivery, Fort Collins, CO
            80523, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.1
            Genome Coverage                   :: 246
            Sequencing Technology :: Illumina NextSeq;Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015689395.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 02:47:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,059
            CDSs (total)                      :: 1,970
            Genes (coding)                    :: 1,933
            CDSs (with protein)               :: 1,933
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 16 of 37
            Pseudo Genes (incomplete)         :: 20 of 37
            Pseudo Genes (internal stop)      :: 16 of 37
            Pseudo Genes (multiple problems)  :: 14 of 37
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:11:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 130030
            Orig. end                         :: 140282
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP065054.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10252
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10252
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10252
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5252](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5252)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            182..505
                     /db_xref="GeneID:83703981"
                     /gene="comGC"
                     /locus_tag="I2432_RS00760"
                     /old_locus_tag="LJFMMFNO_00151"
     CDS             182..505
                     /codon_start=1
                     /db_xref="GeneID:83703981"
                     /gene="comGC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622142.1"
                     /locus_tag="I2432_RS00760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00151"
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="WP_206158899.1"
                     /transl_table=11
                     /translation="MKMRLSTLKDQAVQAFTLLEMLLVLLIISVLMLLFVPNLSKQKDK
                     VTDTGNAAVVKLVENQAELYELSHGEKPSLKQLQEDGSISDKQQKAYQDYYAKHKDETK
                     KLN"
     gene            483..908
                     /db_xref="GeneID:83703982"
                     /gene="comGD"
                     /locus_tag="I2432_RS00765"
                     /old_locus_tag="LJFMMFNO_00152"
     CDS             483..908
                     /codon_start=1
                     /db_xref="GeneID:83703982"
                     /gene="comGD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678812.1"
                     /locus_tag="I2432_RS00765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00152"
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="WP_012514783.1"
                     /transl_table=11
                     /translation="MKLRSSIKAFTLLEGLLTLFLISFMLLCLSLPVSDSYARVEEHLF
                     FMRFEQAYRHLQKLSILRQKEHVLVLKPHYIKAEEAILPLPKQVKLKSTQTLTIDKLGG
                     NHSLARVVFENRDRQITYQFYLGSGNYQKTSQSLHRS"
     gene            865..1155
                     /db_xref="GeneID:83703983"
                     /gene="comGE"
                     /locus_tag="I2432_RS00770"
                     /old_locus_tag="LJFMMFNO_00153"
     CDS             865..1155
                     /codon_start=1
                     /db_xref="GeneID:83703983"
                     /gene="comGE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678813.1"
                     /locus_tag="I2432_RS00770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00153"
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="WP_206158900.1"
                     /transl_table=11
                     /translation="MVIIKKQVKAYIALEGLIATGLILVIVTLILSALHYSQQELAACR
                     QKQELLNTAVMAMQTKQRVLSLNGCHIKLLSHQKGIAIVEKDQTIIRIERQ"
     gene            1142..1576
                     /db_xref="GeneID:83703984"
                     /gene="comGF"
                     /locus_tag="I2432_RS00775"
                     /old_locus_tag="LJFMMFNO_00154"
     CDS             1142..1576
                     /codon_start=1
                     /db_xref="GeneID:83703984"
                     /gene="comGF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677237.1"
                     /locus_tag="I2432_RS00775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00154"
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="WP_012677237.1"
                     /transl_table=11
                     /translation="MKDSRLKAFTLIECLIALLVISGSLLVYQALTKSLMVSERYLAAN
                     DQDNWLLFSQQLRAELSGTTLQGVSNNRLYVEKDKKTLSFGQVKSHDFRKAAGNGRGYQ
                     PMLFGLSSSQITAVGQQVIIKLKWQSGLERTFIYAFQEKG"
     gene            1554..1916
                     /db_xref="GeneID:83703985"
                     /gene="comGG"
                     /locus_tag="I2432_RS00780"
                     /old_locus_tag="LJFMMFNO_00155"
     CDS             1554..1916
                     /codon_start=1
                     /db_xref="GeneID:83703985"
                     /gene="comGG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678815.1"
                     /locus_tag="I2432_RS00780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00155"
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="WP_206152302.1"
                     /transl_table=11
                     /translation="MHFKRRVKAGVLLYALLMASVFLLLLQAYLSHISQLHQEYQAQMD
                     YAKAQLMAEMVYQEQPPSDGQIAFNCGQASYKSHKQHFIIEIKLPHQQLYQFSYPKVQQ
                     VSQTSKKEPELRQSLP"
     gene            1978..2931
                     /db_xref="GeneID:83703986"
                     /locus_tag="I2432_RS00785"
                     /old_locus_tag="LJFMMFNO_00156"
     CDS             1978..2931
                     /codon_start=1
                     /db_xref="GeneID:83703986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678816.1"
                     /locus_tag="I2432_RS00785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00156"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_037580627.1"
                     /transl_table=11
                     /translation="MNFEKIEQAYELILENSQLIENDLKTHIYDAIVEQNSFYLGAQGA
                     SPQVAKNIETLKALQLTKEEWRQAYQFVLIKAGKTEPLQANHQFTPDAIGFIMLYILET
                     LSSQESLDVLEIGSGTGNLAHTILNHSHQAIHYLGIELDDLLIDLSASIAEIMGSSAQF
                     IQEDAVRPQLLKESDMIISDLPVGFYPNDDIASRYQVASSDEHTYAHHLLMEQALKYLK
                     KDGFAIFLAPVNLLSSPQSHLLKQWLKGYAQVAALITLPEAVFGNPANAKSIIVLCKQS
                     NRFAETFVYPIRDLKSVDNVRDFMENFKNWKRDNVI"
     misc_feature    2965..2976
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 19.87"
                     /tool="antismash"
     gene            2991..4190
                     /db_xref="GeneID:83703987"
                     /locus_tag="I2432_RS00790"
                     /old_locus_tag="LJFMMFNO_00157"
     CDS             2991..4190
                     /EC_number="2.7.2.1"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008776 - acetate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83703987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678817.1"
                     /locus_tag="I2432_RS00790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00157"
                     /product="acetate kinase"
                     /protein_id="WP_165627047.1"
                     /transl_table=11
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEKVLAQGIIERIGLTDSISTVK
                     FDGKKEEHILDIPDHTEAVKRLLNDLIHFGIIGTYDEITGVGHRIVAGGEYFKESVVVD
                     DKVVEQVEELAALAPLHNPGAAAGIRAFRKILPDITSVCVFDTSFHTTMQKHTYLYPIP
                     QKYYTDYKVRKYGAHGTSHKYVAEEAAKMLGRPLDELKLITAHVGNGVSITANYHGQSV
                     DTSMGFTPLAGPMMGTRSGDIDPAIIPYLIAQDPELKDAADVVNMLNKQSGLGGVSGIS
                     SDMRDIEAGLQANNPDAVLAYNIFIDRIKKFIGQYFAVLNGADALVFTAGMGENAPLMR
                     QDVVNGLSWFGMEIDPEKNVFGYRGDISTAASKVKVLVISTDEELCIARDVERLKQTIS
                     "
     gene            complement(4801..4983)
                     /db_xref="GeneID:83703988"
                     /locus_tag="I2432_RS00795"
                     /old_locus_tag="LJFMMFNO_00158"
     CDS             complement(4801..4983)
                     /codon_start=1
                     /db_xref="GeneID:83703988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987564.1"
                     /locus_tag="I2432_RS00795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00158"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_206158901.1"
                     /transl_table=11
                     /translation="MNNFLELQDQALTNVYGGGGNIGSAIGGCIGGVLLAAAGGPITGG
                     AAAFVCIASGIATGL"
     gene            complement(5001..5252)
                     /db_xref="GeneID:83703989"
                     /locus_tag="I2432_RS00800"
                     /old_locus_tag="LJFMMFNO_00159"
     CDS             complement(5001..5252)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83703989"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858211.1"
                     /locus_tag="I2432_RS00800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00159"
                     /product="bacteriocin class II family protein"
                     /protein_id="WP_206158902.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 6.2e-16,
                     bitscore: 55.4, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKRSKKLTSEELADIIGGYNATDCRNHLIGGISGGAITGGVGSG
                     LVTLGVGGPAGAFVGAHIGAIAGGITCIGGMIGNNIKF"
     misc_feature    complement(5726..5737)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: strong, score: 19.22"
                     /tool="antismash"
     gene            5766..7808
                     /db_xref="GeneID:83703990"
                     /locus_tag="I2432_RS00805"
                     /old_locus_tag="LJFMMFNO_00160"
     CDS             5766..7808
                     /codon_start=1
                     /db_xref="GeneID:83703990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677242.1"
                     /locus_tag="I2432_RS00805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00160"
                     /product="BglG family transcription antiterminator"
                     /protein_id="WP_115250224.1"
                     /transl_table=11
                     /translation="MVDSKAVVILMTLMTYQDLSLYELSVKTKFTIREIKDRIDELNDF
                     LAQHELSELQTTRSGYRISQQLKAESDHLFTLINAEQVYLSQAERINLIYLYTFCRRDF
                     VSNSHYQDFLKVSKNTALTDIKALRQKLADVDLELRYTRASGYTLVGDELDKHRLALAM
                     IATLLKSPIGLWALDYILASWHYQVSFEALEKRVSHFYQTFQMSPILDRLQACLYAFIF
                     ILCRYQRRVDRVKQRPVVVSEQLDQLTALLVSEIMQQLSLRYQLFEADEAYFQLLLAGC
                     FEGEGTVSDDFFESLTAAIVDQMQGVALLSFSQSDQLKEDLKRHLIPAYYRLRYGLPSD
                     NDYTLRVKDSYSELFDLVKQSLEPLAKELDSPIPDSEIAYFVLHFGGYLKQQGKDFHRQ
                     AYRAVIVCPNGVSSSLMIKEHLKLLFPDIAFRGLSRLDQLGQLDESGYDLVFSTIRLET
                     DKPFYLVPMIMTDEQAFQLVSLVNRDFPDIGYRLEVEELLHLISSYAVIHQKEKLRFAL
                     QQYLRQDVKRKDIRPVLQDLITKKTYQSTSEQLGWREAIYLAAQPLLESGQIQASYQEA
                     MIAKVEEFGPFINLGKGIAIPHARPEDGVNQVGMSMLVLEHPIYLLDDPKQEIRLLICI
                     AAIDNETHLKALSRLTTILRDNEQVKALLASKSFEDIKTIIAEEA"
     gene            7810..8097
                     /db_xref="GeneID:83703991"
                     /locus_tag="I2432_RS00810"
                     /old_locus_tag="LJFMMFNO_00161"
     CDS             7810..8097
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83703991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678822.1"
                     /locus_tag="I2432_RS00810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00161"
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="WP_012514792.1"
                     /transl_table=11
                     /translation="MALRIGTACGSGLGSSFMVQMNIESVLKDLGVTGVEVEHYDLGGA
                     DPTAADVWIVGRDLADSAGHLGDVRILNSIIDMDELTSLVKAICQEKGLV"
     gene            8110..9453
                     /db_xref="GeneID:83703992"
                     /locus_tag="I2432_RS00815"
                     /old_locus_tag="LJFMMFNO_00162"
     CDS             8110..9453
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:83703992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677244.1"
                     /locus_tag="I2432_RS00815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LJFMMFNO_00162"
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="WP_206158903.1"
                     /transl_table=11
                     /translation="MTFLLDIVSTPAILVALIAIVGLLLQKKPAPDIVKGGIKTFVGFL
                     VVSGGAGIVQGSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFTGMIFN
                     ILIARFTRFKYIFLTGHHTLYMACMIAVIMSVAGFTSVSLIVFGGLALGIIMSISPALV
                     QRYMIQLTGHDKVALGHFSSLGYWLSGFVGGLVGDKTKSTEDINFPKSLAFLRDSTVSI
                     TLSMAIIYLIVAIFAGPAWISKELSSGTHGLVFALQLAGQFAAGVFVILAGVRLILGEI
                     VPAFKGISEKLVPHSKPALDCPIVYPYAPNAVLIGFVSSFIGGLVSMAVMIASGTTVIL
                     PGVVPHFFCGATAGVIGNASGGVRGATVGAFLQGVLISYLPIFLMPVLGGLGFEGSTFS
                     DADFGLSGILLGTLRQMGGVTAIVIGIFVILAGLVGLSVVGKSAAKEE"
     misc_feature    9846..9859
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.54"
                     /tool="antismash"
ORIGIN
        1 aaagcttggt cgggagctag atatctatgc ccaagaaagc tgggagacct tttttagtca
       61 gctcaatcgg gcaacacaat ggatccagcc gattattttt ttagtggttg ctgtggttat
      121 tgtgatgatt tatgctgcta ttttattacc aatttatcaa catatgggag atgtttttta
      181 aatgaaaatg agattatcaa ccttaaaaga tcaagctgtt caggccttca ctttactgga
      241 aatgcttctg gttttgttaa tcattagtgt tttgatgctg ttatttgttc caaatttaag
      301 taaacaaaag gataaggtga cagatacagg caatgctgcg gtggtaaagc tagttgaaaa
      361 tcaggcagag ctttatgaat tatcacatgg ggaaaagcca agcctgaagc aactgcaaga
      421 ggatggaagc atcagtgaca agcaacaaaa ggcttatcag gattattatg caaagcataa
      481 agatgaaact aagaagctca attaaagctt ttaccctctt agagggctta ctgacgcttt
      541 ttttgattag ctttatgctg ctgtgcttgt cattaccggt atcagatagc tatgctaggg
      601 ttgaggagca tctctttttc atgcgctttg aacaggccta taggcaccta cagaagctat
      661 cgatattacg tcaaaaagag catgtgctgg tcttaaagcc acattatatt aaggccgaag
      721 aagctatttt gcctctgcca aagcaggtca agctaaaatc tacccagacc ttaaccattg
      781 ataaactggg cggcaatcat tcgctggcaa gggttgtctt tgaaaataga gatcggcaga
      841 ttacttatca attttattta gggagtggta attatcaaaa aacaagtcaa agcctacatc
      901 gctcttgaag gcttgatagc aacaggactt attcttgtta ttgtaacact gatcctgtca
      961 gccctgcatt atagccagca agagctagca gcctgtcgtc aaaagcaaga gctattgaat
     1021 acagctgtga tggctatgca gacaaaacaa agggtattaa gccttaatgg ttgtcatatt
     1081 aagctattat cacatcagaa gggtatcgct attgttgaaa aagaccaaac gattattagg
     1141 attgaaagac agtaggttaa aggctttcac cttgatagag tgccttattg ccttgcttgt
     1201 catctcaggc tctttattag tttatcaggc cttaaccaag agccttatgg tgagtgagag
     1261 gtatctagca gcaaatgatc aggacaactg gcttttgttt tcccagcaat tgcgagcaga
     1321 gctttcaggt actaccttac agggtgtctc caataatagg ctatatgttg agaaagacaa
     1381 gaaaactctg tcctttggac aggtcaaaag ccatgatttt agaaaagcag ctggcaatgg
     1441 tcgaggctat cagcccatgc tgtttggctt gtcaagtagc caaataacag cagtaggtca
     1501 gcaggttatc atcaagctga agtggcaaag cggcttagaa aggaccttta tttatgcatt
     1561 tcaagagaag ggttaaggca ggagtgcttt tgtatgcttt gctgatggca tcagtctttt
     1621 tattactgtt acaggcttat cttagtcata ttagccagct acatcaggaa tatcaggctc
     1681 agatggacta cgcaaaggct cagctaatgg cagaaatggt ctatcaggag cagcctccta
     1741 gtgatggtca gattgccttt aattgtggtc aggctagcta taagtcgcat aagcagcatt
     1801 ttataattga gatcaaatta ccacatcagc agttatatca attcagctat cctaaagttc
     1861 agcaggtctc tcagacttct aaaaaggagc ctgagcttag gcaatcactc ccataactaa
     1921 gggagcctgg ctagtggtat tcttttcatt tcagccttat tttgatatga tagacgtatg
     1981 aattttgaaa agattgaaca agcctatgag cttatattag aaaatagcca gctgattgaa
     2041 aatgatctaa aaacgcatat ctatgatgct attgttgagc agaattcttt ttatctggga
     2101 gcccaaggag caagtcctca ggttgctaaa aatattgaga cgttgaaggc cttgcagtta
     2161 accaaggagg agtggcgcca ggcttatcag tttgttttga tcaaggctgg gaaaacagag
     2221 ccattacagg ccaaccacca atttacccca gacgcgatcg gctttatcat gctttatatt
     2281 ttggagacct tgagctcaca agagtcactt gatgtgcttg agattggcag tgggacaggc
     2341 aatctggctc acaccatttt aaaccattca caccaagcga ttcattattt aggcattgag
     2401 cttgatgatt tattaattga tctatcagct agtattgctg aaatcatggg gtcgtcagcc
     2461 cagtttattc aagaggatgc tgtcagacct cagttgttaa aagaaagtga tatgatcatt
     2521 agtgatttgc cagttggctt ttatcctaac gatgatattg ccagcaggta tcaggtggct
     2581 agctcagatg agcataccta tgcccatcat ttactgatgg agcaggcctt aaagtacctg
     2641 aaaaaagatg gctttgctat tttcttagca ccggtaaatc ttttgagcag cccacaaagt
     2701 cacctcttaa aacaatggct gaagggctat gctcaggttg cggccttgat tactttgcct
     2761 gaggctgtgt ttggaaatcc agctaatgca aaatcgatta ttgttctttg taagcaatcg
     2821 aatcgctttg cagaaacctt tgtttacccc attagggatt tgaaatctgt tgataatgtt
     2881 cgtgatttta tggaaaactt caaaaattgg aaacgggata atgttattta acggaatttt
     2941 ttgatataat agaaaagcat tgcagaaaac gatttcaaga gaggtaaaac atgtcaaaga
     3001 caattgcaat taatgcagga agctccagct taaaatggca gctatatcaa atgccagagg
     3061 aaaaggtatt agcacaggga attatcgaac gtatcggtct gacagactca atctctactg
     3121 ttaaattcga tggcaaaaaa gaagagcata ttcttgatat tccagatcat acagaggctg
     3181 ttaagcgctt gttgaatgat ttgattcatt ttggcattat tggtacgtat gacgagatca
     3241 caggtgttgg gcaccgtatt gttgcaggtg gtgaatactt taaggaatct gtggttgttg
     3301 atgataaggt tgttgagcag gttgaggagt tagcagcctt ggcacccttg cacaatccgg
     3361 gggcagcagc aggtattcgt gcctttcgta agattttgcc ggacattacg agtgtttgtg
     3421 tgtttgatac gtcgtttcat accacaatgc aaaagcatac ctacctttat ccaattcctc
     3481 aaaaatatta tacagattat aaggttcgca agtacggggc gcatgggact agccataaat
     3541 atgttgctga ggaagcagca aaaatgctgg gtcgccctct agacgaatta aagctcatta
     3601 cagcccatgt tggtaatggt gtgtctatca cagccaatta tcatggtcag tcagtggata
     3661 cttctatggg ctttacaccg cttgctggtc cgatgatggg gacacgctct ggtgatattg
     3721 atcctgctat tattccttat ttgattgctc aggatcctga gctaaaggac gcagcagatg
     3781 tggtcaatat gcttaacaag caatctggat taggtggtgt gtctggtatt tccagtgata
     3841 tgcgtgatat tgaggctggc ttgcaggcta acaatccaga tgccgtctta gcttacaata
     3901 tctttattga tcgtatcaag aaatttatcg gacaatactt tgcggtatta aatggtgcag
     3961 atgccttggt ctttaccgca ggtatgggtg agaatgcgcc cttgatgcgc caagatgtcg
     4021 ttaatggctt gtcatggttt ggtatggaaa ttgatccaga gaaaaatgtg tttggttatc
     4081 gtggtgatat ttcaacagca gcttcaaagg tgaaggtgct tgtcatctca actgatgaag
     4141 agctgtgtat tgcgcgtgac gttgaacgct tgaaacagac tatatcataa tctgataata
     4201 taatcttagc tcagctgatc acttttaaaa gggttgagtt tggggcaaaa gactaacgat
     4261 caatctaaaa aaacacgaac aaaagtatat ttctagtcaa taggtttata cttttgttcg
     4321 ttttgctatg ttaatcactg tggatcgagg tttcataaaa agagtctgag acaaaaagtc
     4381 atctcagtcg agaaaaatag gcaattattt ttcgtgtaag ttcctatttt agtattgaat
     4441 cattttttta ttgtacttga taagagtatt agctataaga actaatctca tatcagtttt
     4501 aatccgctgt ttatctatta aatattcttt gatattaacg atatgatctt agataataag
     4561 tttaaaagca aaatgcttac taaaaataaa gaggtaatag cattaaagca atattacctc
     4621 aaagattcta cagatatgtt ttggttattt atttaaaaat caattttatg aatatataaa
     4681 ctaatattgt gatagcaatg accttaccag catttgcctt taccttttga tctttcaaaa
     4741 agacttgtga taatataaga caaattaatc cagatataat agttatcgcc atatgattaa
     4801 ctacaagcca gtcgcaattc ctgaagcaat acaaacaaat gctgctgcac cacctgttat
     4861 tggacctcca gctgctgcaa gtaatacacc acctatacaa ccacctatag cagagccaat
     4921 gttcccacct ccgccgtata cattagttaa cgcttgatct tgtaattcta aaaaattatt
     4981 catatacttt tacctttata ttagaatttt atattatttc ctatcatgcc acctatacaa
     5041 gttattccac ctgcaatcgc cccaatatga gctccaacaa atgctccagc aggccctcct
     5101 acacctaatg taaccagacc tgaaccaact cctccagtta tagcaccacc actaatacca
     5161 ccaattaaat gatttctaca gtcagtagca ttatagcctc ctataatatc ggctaattct
     5221 tcacttgtta attttttact tcgtttaaac atatttattt aaaaagcctc ccattctata
     5281 tttgtcaaac tgctgcaatc taacaaacta aatgtagcac atcctatcat gatttgtcaa
     5341 tttagaagcg gttacattag agtaaataga gtttttcttt ttacctaaat taattttaat
     5401 ttaagtaaaa agaatattat gttactattt gacctatggt attattttcg ttttatacct
     5461 agagttatca catgctgatg aggtcaatca gtttttttga ttatttggaa catttttgac
     5521 ggttattact ccaaagagtt taagctttgg cttggacctg gaaaatcatg aggtaactgc
     5581 ttagcggtag gggtggattt gtttgccctt tattgatagt tagaggagct agtagcctgt
     5641 ttagattggc tggtctgcaa tcttgagccc aagggctgtt gatggtgctg gataagaaat
     5701 tgtgcccaat gggggctagt ttattgtaat cgttttcgat agaatgactt tagaaaggaa
     5761 atcaaatggt tgatagcaaa gcagttgtca ttttgatgac tttgatgact tatcaagact
     5821 tatcacttta cgagctgagt gttaagacaa aattcaccat tagagagatt aaggaccgta
     5881 ttgatgagct gaatgatttt ttggctcagc atgagctctc agagctgcag acaacaagat
     5941 ctggttatcg gatcagtcag cagcttaagg ctgagtcaga tcatttgttt acgttgatta
     6001 atgctgagca ggtctatctt tctcaggcag agcggatcaa tcttatctat ctgtatacct
     6061 tttgccgtag ggattttgtt tcaaatagtc attatcagga ctttttaaag gtcagcaaga
     6121 acacagcatt gacagatatt aaggccttgc ggcagaagct ggcagatgtt gacttggaat
     6181 taaggtatac cagagcaagt ggctatactc tagttggtga tgagcttgat aagcaccgat
     6241 tagctttggc tatgattgct actctgctta aatctcctat tggcttgtgg gccttggatt
     6301 atattttggc ttcttggcac tatcaggtgt cgtttgaagc gcttgaaaaa cgtgtgagcc
     6361 atttttatca gacctttcag atgtctccaa tccttgatcg tctgcaggcc tgtttgtacg
     6421 ctttcatctt tattttatgt cgttaccaaa gacgggtgga tagagtcaag caaagaccgg
     6481 tagtagtttc tgagcagctg gatcagctaa cagccctttt ggtgtcagaa ataatgcagc
     6541 aattgtctct taggtatcag ctatttgaag ctgatgaggc ctattttcaa ttgctgttag
     6601 cgggctgctt tgagggtgag gggactgtca gtgatgattt ctttgaatca ctaaccgctg
     6661 cgattgttga tcaaatgcag ggggttgctt tactaagctt cagccaatca gatcagctca
     6721 aggaggattt gaagaggcat ttgatcccag cctattaccg tttgcgctat ggtttgccaa
     6781 gtgataatga ttataccttg cgggttaagg atagttattc tgagctcttt gacttggtaa
     6841 agcagtctct ggagccttta gccaaggagc tggatagtcc cataccagat agtgagattg
     6901 cttattttgt tcttcatttt ggaggctatt taaagcagca gggaaaagat tttcatcgtc
     6961 aagcctatcg ggctgtgatt gtctgtccta atggtgttag ttcttcttta atgatcaagg
     7021 agcatttaaa gcttttattt cctgatattg cttttcgggg actttcaagg cttgatcagc
     7081 ttgggcaatt agatgagagc ggctatgatt tggttttttc aacgatacga ttagagactg
     7141 ataagccctt ttatttggta cctatgataa tgactgatga gcaggctttt cagctggtta
     7201 gcttggttaa tcgtgatttt ccagatattg gctatcggct agaggttgaa gagctgctcc
     7261 atcttatcag ctcatatgcg gtcattcatc aaaaagaaaa gctgcgcttt gctttgcagc
     7321 aatatttaag acaagatgtg aaaagaaagg atattcgacc agtgttacaa gacttaatta
     7381 ccaagaaaac ctatcaatca acctcagagc aattggggtg gagagaggct atttatttgg
     7441 cggctcagcc cttgttagag agtgggcaga ttcaagcgag ctaccaggag gctatgattg
     7501 ctaaggttga ggagtttggt ccttttatta atctaggtaa ggggattgct atcccacatg
     7561 cgaggccaga ggatggtgtg aatcaggtag gtatgtcgat gcttgtttta gagcacccga
     7621 tttatttgtt agatgatcct aagcaggaga ttcgtttgtt aatttgtatt gctgctattg
     7681 ataatgaaac acatctaaag gccctgtctc ggctgacgac cattttaaga gacaatgagc
     7741 aggtaaaggc tttacttgcg tctaaatcat ttgaggatat taaaactatt attgcagagg
     7801 aggcataaaa tggcattgag aattggtaca gcctgtggtt caggcttagg gtctagcttt
     7861 atggttcaga tgaatatcga atccgtatta aaggacttgg gagtaaccgg tgttgaggtg
     7921 gagcattatg atcttggcgg cgcggacccc acagcggcag atgtgtggat tgtagggcgt
     7981 gatttggcag actcggcagg gcatctggga gatgtgagaa ttttaaatag tatcattgat
     8041 atggacgaat tgactagtct ggtaaaggct atttgccagg aaaaggggct tgtctagaag
     8101 gaggataaga tgacattttt acttgatatt gttagcacac cagctatttt ggttgccttg
     8161 attgctattg ttggtctctt gcttcaaaaa aagcctgctc ctgacattgt taagggaggc
     8221 attaagacct ttgttgggtt tttagtggtt tcaggtggtg caggtattgt gcaaggctca
     8281 ctcaatcctt ttggaacgat gtttgagcat gcctttcatc tgtctggggt tgtgccaaat
     8341 aatgaggcta ttgttgcagt tgctttgacg acctatgggt cagcgacagc tctcattatg
     8401 tttacaggaa tgatctttaa tattttgatt gctcgcttta cccgctttaa gtatatcttc
     8461 ttgacggggc atcatacgct ttacatggcc tgtatgattg ctgtgattat gtctgttgca
     8521 ggctttacat cagtgtcctt gattgtgttt ggtgggctgg ccttagggat tatcatgagt
     8581 atttcaccag ctcttgtgca aagatacatg attcagctga cgggacatga caaggtcgct
     8641 ttggggcatt ttagctctct tggttattgg ttaagcggtt ttgtgggagg tcttgttgga
     8701 gacaagacca agtcaacaga ggacatcaat tttccaaaga gccttgcctt tttgcgcgat
     8761 agtacggtaa gcattaccct gtcaatggca atcatttatt tgattgtagc tatttttgca
     8821 ggtcctgctt ggattagcaa ggaattatcc tctggcacac atggcttggt ttttgcttta
     8881 caattggcag gacaatttgc tgcaggtgtc tttgtgatat tagctggtgt tcggctgatc
     8941 ctaggtgaaa ttgtgccagc cttcaagggc atttctgaaa aattagtgcc acattctaag
     9001 ccagccttgg attgtcctat tgtttaccct tatgccccaa atgcagtgct aattggcttt
     9061 gtatctagct ttattggtgg cttggtgagt atggctgtga tgatagcaag tggaaccacg
     9121 gtgattttgc ctggtgttgt gcctcatttc ttctgtggtg caacagctgg tgttattggt
     9181 aatgcctctg gaggtgtgcg cggtgcaact gtgggagcct tcttacaggg tgttcttatt
     9241 agttatcttc ctattttctt gatgcctgtt ttaggtggtc taggctttga gggctcaaca
     9301 ttctcagatg ccgattttgg cttatcaggt attctattag ggacacttcg tcagatgggc
     9361 ggtgtgacag ctattgtgat tggcattttt gtgatattag ctggacttgt tgggctttct
     9421 gttgttggaa aatcagcagc taaggaggaa taagtatggc tttgagtgat gagcagctaa
     9481 cagatatgaa gcgatttgca gcagagattc gcctaaatac cttggagact cttaatcacc
     9541 ttggctttgg tcattatggt ggtagcttgt ctattgtaga attgctggct gtgctttatg
     9601 gagaaatcat gcctatgaca cctgagctct ttgcccagtc agatagagat tacttggtct
     9661 tatcaaaggg acatgcgggt cctgccctct atagcactct ctatctcaag ggattctttg
     9721 ataaggcctt tttacagtct cttaatacca atggaacaag cctgccctcg cacccagatc
     9781 gtctgctaac gcctggtatt gatatgacga caggctctct tggtcaggga attagcgtag
     9841 caacgggcat tgcctatgct caagcgcttg agcagtcacc atattatacc tatactatcg
     9901 ttggtgatgg ggaattaaac gagggccaat gctgggaggc tattcagctt gcggcccatc
     9961 agcagttaag tcatttcttt gtctttgttg atgacaataa gaagcaatta gatggaacaa
    10021 ccaaggcaat ctgtcgtcca ggtgattttg ttgaaaagtt tacggcattt ggctttgaaa
    10081 gtttgcgtgt agatggtaga gatgttcagg ctatttttga agccatcagg gctttgcagg
    10141 cgtcatcgag tcataagccc aagtgtatcg tcctagatac tgttaaaggt caaggggtca
    10201 gtgagctaga gtcaatggca gctaaccacc atctaagacc aacagcagcc ga
//
