LOCUS       NZ_JADPFA010000004     12097 bp    DNA     linear   CON 28-JAN-2024
DEFINITION  Streptococcus salivarius strain D43t1_170807_H4
            NODE_4_length_215930_cov_33.4937, whole genome shotgun sequence.
ACCESSION   NZ_JADPFA010000004
VERSION     NZ_JADPFA010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus salivarius
  ORGANISM  Streptococcus salivarius
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 215930)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Quantification of discrete gut bacterial strains following fecal
            transplantation for recurrent Clostridioides difficile infection
            demonstrates long-term stable engraftment in non-relapsing
            recipients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 215930)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, 1470 Madison Ave, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 27.95x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 10:18:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,053
            CDSs (total)                      :: 2,002
            Genes (coding)                    :: 1,964
            CDSs (with protein)               :: 1,964
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 13 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 12 of 38
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:50:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 75153
            Orig. end                         :: 87250
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADPFA010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12097
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12097
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12097
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7097](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7097)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    80..98
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.58"
                     /tool="antismash"
     gene            complement(801..1703)
                     /locus_tag="I4U27_RS06610"
     CDS             complement(801..1703)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607957.1"
                     /locus_tag="I4U27_RS06610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="WP_014633605.1"
                     /transl_table=11
                     /translation="MKLKKILGITGLAVASTVALAACGAGGNKSAKDDKTLTVGIMTLD
                     DTTEPLWDKVKELAKDKGVTIELKEFTDYNQPNEALKNGEIDVNAFQHIYFLNNWNKEN
                     KGDVVAAADTLLSPIHLFSGTENGKAKYKDVKELPEGATISVPNDGTNESRALTLLQTA
                     GLIKLDVKDGELATIKNISENPKKLDIKEISAEQAAQTLSSVDAAVVNNSYAQQQNVNY
                     DTTLFQEEPSQDLKDWVNIIAANKDWEKSNKADAIKTLIKAYQNDEVAQIIYDASNKVD
                     LPAWKGAPTQDQLKANSKK"
     misc_feature    complement(1771..1786)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 23.45"
                     /tool="antismash"
     misc_feature    complement(1837..1855)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.78"
                     /tool="antismash"
     gene            complement(1863..2711)
                     /locus_tag="I4U27_RS06615"
     CDS             complement(1863..2711)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011225460.1"
                     /locus_tag="I4U27_RS06615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="WP_014633606.1"
                     /transl_table=11
                     /translation="MKLKKILGITGVAIASVALLAACSSKSSKEASKSSGAKETINFAT
                     VGTTAPFSYEENGELTGYDVEVAKAVFKDSDKYEVKFQKTEWSSVFTGLDSAKYQMAGN
                     NISYSEERDQKYLFSYPIGTTPAVLTVPKDSNIKKYDDIAGHSTQVVQGTSTATQLTEF
                     NDKHSDKPVELNYTNENITQMLTSLNEGKYDFKIFDAPTVNAIIKNNKLSNLKTIELKS
                     DQQPYIYFLFADNQKDLQKFVNKRLEELQKDGTLAKLAEKFLGNKDYIPTKDALKVPSK
                     K"
     misc_feature    complement(2918..2938)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 27.09"
                     /tool="antismash"
     misc_feature    complement(3024..3041)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.94"
                     /tool="antismash"
     gene            3098..4492
                     /locus_tag="I4U27_RS06620"
     CDS             3098..4492
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883799.1"
                     /locus_tag="I4U27_RS06620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4153 domain-containing protein"
                     /protein_id="WP_230311014.1"
                     /transl_table=11
                     /translation="MMETNNYTTDLQLVSQEYSDEKTSLQTKTKKFLEHFSTKDIWKIK
                     GLIVACLVSCFLYVTYLTHFSFALPATLVLISTIELGCRLLGGKAKGLRLESGIFMVTS
                     LLQGLQISLWGLHDNWGSAQILCLHASILLYLMARTGQSSQDRLGIMVWTDLIYTSCIF
                     PLKYFFIGAWTLLYKTAPKQELSEEEKHVIKNHKWMILISIMIALVLTVFAASQLSQVL
                     PAFGQLWRQFFQTLTSLLDFTLTFKDNGLLFSRLISSIPLALGLFAQLAATYLAKEKPL
                     SQKRFEGHLKATRQFPKVAAYIVLGSLCFTYALFILTATSQMGELLSLNQASIPAHQAS
                     HIAVQGFWQLVRVSTLNLGILAGLYIFSKENFFTHKGLRFLSTLLFTFASLFALIAGWK
                     LFGVYMALYGVTPRRLLSGWFVTVLFIWCVLTLIRLYKPIQATRLAIFYATISFTLVNL
                     LGNFIL"
     gene            complement(4544..4876)
                     /locus_tag="I4U27_RS06625"
     CDS             complement(4544..4876)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097990.1"
                     /locus_tag="I4U27_RS06625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF805 domain-containing protein"
                     /protein_id="WP_014633880.1"
                     /transl_table=11
                     /translation="MLEAYKNFWLGYIDFYGRTKRSDYWWVVLINGVIGTLIFLLMDFL
                     GETKAAGFTLTAFILFIAATALPTISMQIRRLRDANFNVAWIVLKATPLLVVLWVMYAF
                     PTRDSK"
     misc_feature    complement(4838..4858)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 25.1"
                     /tool="antismash"
     gene            complement(5001..7097)
                     /locus_tag="I4U27_RS06630"
     CDS             complement(5001..7097)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535846.1"
                     /locus_tag="I4U27_RS06630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_195914714.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.9e-44, bitscore:
                     144.4, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 4e-260, bitscore:
                     858.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKYPYISQVDQRDCGVAALAMILKHYGSSYSLAYLRELAQTSRE
                     GTSALGLVEAAKQLGLETQAIRADLDLFKQESLSYPFIAHVVKEGGLQHYYTVFGQIKG
                     KLVIGDPDPSKKVIKIPLEEFAKEWTGVALFFVPGETYTKYKEDVPGLLSFLPILFRRK
                     GLIAVIVLLSFLVTLVNIIGSYYLQSIIDRLIPQEAYTLLTMISLGLCISYLAQQVFTF
                     FKDYLLHRLGNYLSIAVILPYIKHVLSLPISFFSSRRTGEITSRFGDANTIIDALASTI
                     LSIFLDVSIVMTLAVALIVQNQSLFVMTLTVVPLYVLIILAFYKLFEKENYQLMEANSQ
                     VNTAVIDDLRGIETLKSLRVEERRYREIEVKFHDYLKKSLSKAKWQLTQDGLKTGVQLV
                     SNVFILWYGAQLVMEGQLSAGQLITYNMLLNYFTTPLINIINLQSKIQQAKVANNRLQE
                     VYVVDKEEKGKLKELSFKQLAFKGVSHRFSYQQETLSKIDLTIHKGEKIALMGKSGSGK
                     TTLAKMLSGYYTKSSGHVTLDKDAIGHAELRQMVTYVPQQTYVFTGTILENLLLGFEGE
                     VDEKELLKVCQQADILEDIQKMPLGFQTQVSEDGGLSGGQKQRLAIARALLSKQPILIF
                     DEATSGLDSDTESRVMANLTKIKRTMIFIAHRNSVRQHVSRVVTMVSGQIESDSPNFNP
                     FQFI"
     misc_feature    complement(7248..7263)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     gene            complement(7272..8171)
                     /locus_tag="I4U27_RS06635"
     CDS             complement(7272..8171)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883753.1"
                     /locus_tag="I4U27_RS06635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_195187773.1"
                     /transl_table=11
                     /translation="MNIKDSIGLRIKTERECQQMSREVLCLDGAELTVRQLIRIEKGES
                     LPSLDKLSYIAKRLGKNMTDLLDHDNITIPDEYYEMKNRLIKFPTYRNPERIKAKLALI
                     EEVYEKFFELLPEEELLTLDILENILSFTSWEESPKVEEIYEDLFEQVKRKRKFSTNDL
                     LVIDYYFFHLYGRKQYDKKLFERIVKRVLNQEIWTDDVYNIVLFNDLMAIAALKIFHNS
                     FSDFLTVVDKALAVIEKSQFYSYKPSVFVLKAKYELLHKGNKKKAAENYDKAIMFASVL
                     EDSVLEESIRAGKAADGL"
     misc_feature    complement(8315..8324)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            complement(8349..8999)
                     /locus_tag="I4U27_RS06640"
     CDS             complement(8349..8999)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535842.1"
                     /locus_tag="I4U27_RS06640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphatase PAP2 family protein"
                     /protein_id="WP_195187772.1"
                     /transl_table=11
                     /translation="MKHKHTHYIYASFAFLLFVALGYMVKFYPEALTGFDSNIQTWVRG
                     DLPGGLTAFFKMITTLGNTSVQAVIAILAVIWLYLRQYKAEAIFVGTSGVLASTMIMSL
                     KYIYQRPRPSITHLVHAGGYSFPSGHSLGTFMILGAIAIVLTQRLVKKAAKTAVYGITG
                     LLIFLVGLSRIYVGVHYPTDVLAGFTLAFGLLNAIYPTYDRIRFEWRFQSKQK"
     gene            complement(9002..9559)
                     /locus_tag="I4U27_RS06645"
     CDS             complement(9002..9559)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946140.1"
                     /locus_tag="I4U27_RS06645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ECF transporter S component"
                     /protein_id="WP_195187771.1"
                     /transl_table=11
                     /translation="MTNTRKLAYIAILSAVSFLLLYFSFPLIPAADFLKVDFSILPVLI
                     ALVIFDFKSAIGVLLLRSLLKLLLNNGGPGSMIGLPMNFVALGVFVWGLSYFWKKNQTS
                     KNYILGSVWGTVLLTVAMVALNYIYAVPLYAKFANFDIGQFIGLYKYLFAMVVPFNLLE
                     GLIFSIAFALIYAPLKSILVKL"
     regulatory      complement(9640..9762)
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            complement(9849..10358)
                     /locus_tag="I4U27_RS06650"
     CDS             complement(9849..10358)
                     /EC_number="2.1.1.207"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949522.1"
                     /locus_tag="I4U27_RS06650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="WP_195187770.1"
                     /transl_table=11
                     /translation="MSRNHIVLFEPRIPQNTGNIARTCAATNSPLHIIRPMGFPIDDKK
                     MKRAGLDYWDKLDITYYDNLADFMSKNQDGRLHLVTKFADKTYSEEAYADGEDHYFMFG
                     REDTGLPEDFMREHPEKAIRIPMNDEHVRSLNVSNTVCMIVYEALRQQQFAGLELSHRY
                     QNDKLK"
     gene            10636..11985
                     /gene="trkA"
                     /locus_tag="I4U27_RS06655"
     CDS             10636..11985
                     /codon_start=1
                     /gene="trkA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /locus_tag="I4U27_RS06655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="WP_195914715.1"
                     /transl_table=11
                     /translation="MRIIVVGAGKVGTALCRSLVEEKHDVILIEEKEEVLKRLSKRYDV
                     MGFAGNGANFKILEQAEVNNCDVFIAMTDKDEVNMISAVLAKQMGAKETIVRVRNPEYS
                     NAYFKDKNFLGFSLVVNPELLTARYIANTVEFPNALSVETFANGRVILMAFKVTENSQL
                     SGMTMEQFRRKFGNILVCTIDRQGELIIPDGNATMQIGDKIYVTGKRVDMALFHSYIKP
                     KSIKKLLLIGAGRISYYLLNILKNNRIKVKLIEQKMDRAQTFSQVFPEVSVILGDGTAK
                     DLLLEESAASYDAVATLTGVDEENIITSMFLESLGIHKNITKVNRTSLLEIIDPRETTT
                     IVTPKSIAVDTVMHFVRGRFNAKDSSLDALHHVANGRIETLQFQIKENNKIAGKKLSEL
                     ELANGVLIAAIIRNGKSIFPTGEDRLAIGDKIVVVTLLQNVTHIYDLLKR"
ORIGIN
        1 agaatacgtc cctgatcatc tctcatgctt gaaatagcat tgagcaagta ccatgaagcc
       61 gattcaatca cggcaccaaa tttagaatga atatcaacgt cagcactctc gacagacaaa
      121 tcaaaggtaa taattccctt gttgccccct gtgatttcca cttgaccttt actatttcga
      181 tttccttgct cccaaaccaa taggtctgct ggaagaaggt catctcggta tttttccaaa
      241 tatttttcaa ggtcggttga agcagattct tcagctcctt ccatcataaa cgtaatattt
      301 acaggaagat cgccaacctc tcgaatatat ttccgaagag ctgtcaaacg agccgtgatg
      361 tggcccttat catcatcaac accacgacca tacatgtagc ccttacgaac tgtcagacgg
      421 aaaggttcat ctgtccatgg ctgatcatta tccgcaggaa ccgtatcata gtgttgatag
      481 aaaataattg ttttcgcatc tggatttgga ctcttgaatt ttgccaagac aaaaggtgcc
      541 gtataggtct cgtcaattgt cacctctgcg ccaacaccag agaaaatctc accaaggtaa
      601 gcagccacat catagagacc aatattttga gcaaaaatag attttttaga aatcaaagtc
      661 cgcaagactt caaaatagtg ctgagcaatt tcatcgttac ggaattttcg taactgctct
      721 tcttcacttg aaaatgccat caaatcgtct ccttcataca cgacaaaatg gcgtgctctc
      781 acacgccctc tgtctttagc ttatttcttt gaattagctt tcaattgatc ttgtgttggg
      841 gcacctttcc atgctggaag gtcaaccttg ttagatgcat catagatgat ttgtgctact
      901 tcatcgtttt ggtaggcttt aatcaaagtc ttgatagcat cagctttatt tgatttttcc
      961 caatctttat tagctgcaat gatgttaacc caatctttca aatcttgact tggttcttct
     1021 tggaagagag ttgtatcata gttaacgttt tgttgttgtg cgtatgagtt attaacaacc
     1081 gctgcatcta ctgatgaaag cgtttgggca gcttgttcag ctgagatttc tttgatgtcc
     1141 aatttctttg gattctcaga aatgttcttg atagttgcca attcaccatc tttaacatcc
     1201 aatttaatca aaccagctgt ttgaagaagt gtcaaagcac ggctttcgtt tgtaccatcg
     1261 ttaggtactg agattgtagc tccttctgga agctctttaa catctttata cttagctttt
     1321 ccgttttcag tacctgagaa aaggtggatt gggctaagaa gtgtgtcagc tgccgcaaca
     1381 acatcacctt tattttcttt gttccagttg ttcaagaaat aaatgtgttg gaaagcatta
     1441 acatcgattt caccgttttt aagggcttca tttggttggt tgtagtctgt gaactctttc
     1501 aactcgatag taacaccctt gtctttagcc aattctttga ctttatccca caatggctca
     1561 gttgtatcat ccaaagtcat gataccaact gtcaaagttt tatcatcttt agctgattta
     1621 ttaccaccag caccacaagc agccaaagct actgttgaag cgactgccaa tcccgtaatt
     1681 ccaagaattt ttttaagttt cataacgaac tccctttaat tgttttaagt gtaaaattat
     1741 tatcccatag gttgagaatt gtagcaaatt gtttataatt gtatatctct atagttttta
     1801 actataacgt gtcctagatt ttaattatat aaaaaagctg agaatcattc ccagctcctt
     1861 tcttattttt tgcttggaac tttcaaagcg tctttagttg gaatataatc tttatttcca
     1921 aggaattttt cagccaactt agccaaagta ccatcttttt ggagttcttc caaacgtttg
     1981 tttacaaatt tttgaaggtc tttttggtta tctgcgaaca agaagtagat gtatggttgt
     2041 tgatcagatt taagctcaat agtcttcaag ttgctcaatt tattattttt gatgattgca
     2101 ttaacagttg gtgcatcaaa aatcttgaag tcgtacttac cttcattcaa acttgtcaac
     2161 atttgtgtaa tattttcatt tgtatagtta agctcaactg gcttgtctga atgtttatcg
     2221 ttaaattcag tcaattgtgt cgctgtcgat gtcccttgaa caacttgcgt tgagtgtcca
     2281 gcgatatcat catatttctt aatattacta tcttttggaa cagtcaaaac agctggtgtg
     2341 gtaccaattg gatatgagaa gaggtatttt tggtcacgtt cttctgaata agagatattg
     2401 tttccagcca tttggtattt agctgagtca agacctgtaa atactgaaga ccattctgtt
     2461 ttttggaatt tcacttcata tttgtcagag tccttgaaga ctgctttagc cacttccaca
     2521 tcgtaaccag tcaattcacc gttttcttca tatgagaacg gtgctgttgt cccaacagtg
     2581 gcaaagttaa ttgtttcttt agcacctgaa gatttagaag cttcttttga tgatttagat
     2641 gaacatgcag caagcaatgc tactgaagca atggctacac ccgtaattcc aagaattttt
     2701 ttaagtttca taatactctc ctttgagatt gattgttttt attgtaacag aattcactaa
     2761 gcttaactaa tacttaaaca taatcaaggt gataagaaaa tactatcacc tcaaaatatc
     2821 aattgtttga caaagaaaaa gtcatttcca atatctaaga atggacacaa accgttaatc
     2881 tataaaaagc tttctttagt atttgactgt ttctgacaat atagcctgta caatctatca
     2941 ttcaatgtga gcataatatt ttttctcgcc tatttttcca actcattctc cagacctaag
     3001 tctatggact tatttttagt aattttacag ttgggtaaca aagtcaaacg ttttttatga
     3061 taaagtcttt tttggactaa catccaaaag gagaattatg atggaaacaa ataactacac
     3121 tactgattta cagctagtct cccaggaata ttccgatgaa aagactagtc ttcaaaccaa
     3181 aaccaagaaa ttcttggaac atttttcaac aaaagacatt tggaaaatta agggactgat
     3241 tgtcgcctgt cttgtgtcct gcttcctgta tgtcacttac ttgacccatt tttcctttgc
     3301 ccttccagca acccttgtct tgatcagtac gattgagcta ggctgtcgcc tattaggtgg
     3361 aaaagcaaag ggattgcgac ttgaatcggg aatatttatg gtcactagtc tcctccaagg
     3421 cttgcaaatt agtctctggg gtctgcacga caactggggc agcgctcaga ttctttgcct
     3481 tcatgcaagt atcctccttt acttaatggc acgtaccgga caaagtagtc aggaccgttt
     3541 gggaattatg gtttggacag atctcattta taccagttgc atcttcccgc taaaatattt
     3601 ctttatcgga gcctggacac tcctatataa aacagcacct aaacaagaac tctccgaaga
     3661 agaaaaacac gtaatcaaga accacaaatg gatgattctc attagtatca tgattgcctt
     3721 ggtactaact gtattcgctg ctagtcagct tagtcaggtc ttacctgctt tcggacaact
     3781 ttggcgacag ttcttccaga cactaacttc tctacttgat ttcaccctca ccttcaagga
     3841 caacggatta ttgttctctc gtttaataag tagtatccct ctggctctag gactttttgc
     3901 tcaactcgct gcaacctatt tggcaaaaga aaagccactc agtcaaaaac gatttgaagg
     3961 ccatcttaaa gcgacccgtc agttcccaaa agtagcagct tatatcgtac tcggaagcct
     4021 ctgcttcact tatgccctat tcatattaac tgccactagt caaatgggag aactcctttc
     4081 tctcaaccaa gccagcattc cagctcatca agcctcacat attgcggttc aaggcttctg
     4141 gcaattggtt cgagtttcta ccctcaactt aggaatccta gctggacttt acattttttc
     4201 taaagaaaat ttcttcaccc acaaagggct tcgtttctta agcaccctac tctttacttt
     4261 tgctagcctc tttgccctta tcgctggctg gaaactcttt ggcgtttata tggcactcta
     4321 tggtgtgacg ccacgtcgct tattgtcagg ttggtttgtc acagtactct ttatttggtg
     4381 tgtcctaaca cttattcgac tctataaacc tattcaggca actcgtctcg ctatatttta
     4441 tgcaactatc agctttaccc tcgttaacct cttaggtaac tttattttat aagaaaaaaa
     4501 gagtggaact agattaacta gcttcactct ttttatattg gagttatttt gaatctcttg
     4561 taggaaaggc atacatcacc caaagcacta ccagtagtgg tgtcgctttt aatacaatcc
     4621 atgcaacatt gaaattggca tcacgtagac gacgaatctg catagaaatg gtaggaagcg
     4681 ccgttgctgc tataaataaa atgaaagctg ttaatgtaaa gccagcagcc tttgtttctc
     4741 caaggaagtc catcagtagg aaaattaaag taccaataac tccattaatc aagacaaccc
     4801 accaatagtc tgatcgtttt gtacgaccat aaaaatcaat atagcctaac caaaaatttt
     4861 tatatgcctc taacatgttt acacctctta atagtatgct accagtatac tatattaagg
     4921 agataggtgc aagatgtgaa tatttagact acttaagttt tctacatata taattctaca
     4981 tatataatag gaagattatt ttaaataaac tggaatggat tgaaattagg actgtccgac
     5041 tcaatctggc cactcaccat ggtcaccaca cgactgacat gttgacggac actattgcgg
     5101 tgagcaataa aaatcattgt tcgtttaatc ttagtaagat tagccatgac cctgctttca
     5161 gtatcactat cgagaccact ggtagcttcg tcaaaaatca agataggctg cttcgacaag
     5221 agggccctcg ctatcgccaa gcgttgtttt tgaccaccag atagaccacc atcctcagat
     5281 acttgggtct gaaaacctag tggcattttt tgaatatcct ctaagatatc agcttgttgg
     5341 caaaccttaa gaagctcttt ctcgtcaact tctccttcaa atcctaacag cagattttct
     5401 aaaatggttc cagtaaacac ataggtctgc tggggaacgt aagtcaccat ttgacgcagc
     5461 tccgcatggc caatagcatc tttatctagg gttacatgac cactagattt tgtgtaataa
     5521 cccgacaaca ttttcgctaa ggtcgttttc ccagatccac ttttacccat gagggcgatt
     5581 ttctcaccct tatgaatcgt taaatcaatc ttactaagcg tctcttgctg ataactaaaa
     5641 cggtgactga ctcctttaaa agcaagttgt ttaaaagata actctttaag ttttcctttt
     5701 tcttccttgt ccacaacata aacttcttga agacgattat tggcgacttt ggcttgctga
     5761 attttagatt ggagattgat gatattaatt aaaggagttg taaaataatt caacaacata
     5821 ttataggtaa tcaactgacc tgcagatagt tgcccttcca tgaccaattg tgccccatac
     5881 cagaggataa agacattgga aactaactga acccctgtct ttagaccatc ctgcgttaat
     5941 tgccacttgg ccttagacaa ggacttcttc aaatagtcat ggaatttcac ctcaatttcc
     6001 cgataacgtc tttcctcaac cctcaaggat tttaaggttt caatgcctcg caaatcatca
     6061 ataacagcgg tattgacctg actatttgcc tccatcaatt gataattttc tttttcaaaa
     6121 agcttataga atgctagaat gattaaaaca taaagaggaa ctaccgtgag agtcatcaca
     6181 aaaagtgact gattttgtac aataagagcc acagctagag tcatcacaat gctcacatct
     6241 aggaaaatcg ataaaatcgt tgaagctaga gcatcgataa tagtgttggc atctccaaaa
     6301 cgtgacgtga tttcaccagt acgacgtgaa ctaaagaaac taataggtag ggaaagaaca
     6361 tgcttaatat aaggaagaat gacagcaatg gacaagtaat tccccaatcg atgtagaaga
     6421 taatctttga aaaaagtaaa tacttgttga gctagataag agatacatag gcctagcgaa
     6481 atcattgtca gaagagtgta ggcctcctga ggaatcaagc ggtcaataat agattgcaaa
     6541 taataagaac caataatatt aacaagggtc actagaaaac tcagtaagac aatgactgca
     6601 atcaatccct tacgacgaaa caaaattggt aaaaatgaaa gaagcccagg cacatcttct
     6661 ttgtatttag tgtaggtttc tcctggaaca aaaaagagag caactcccgt ccactctttg
     6721 gcaaactcct ctaagggtat tttaatgacc ttcttgctag gatctggatc accaataaca
     6781 agcttccctt tgatttgacc aaacactgta taataatgtt gcagtccacc ttctttaacc
     6841 acatgtgcaa taaaaggata agataggctt tcttgtttaa acaagtctaa atccgcacga
     6901 atggcctgag tttcaagacc caactgctta gccgcttcca ccaaaccaag cgcagaagtg
     6961 ccttcacgag aagtctgagc caattcccgc aaataagcta aactataaga tgaaccatag
     7021 tgtttaagaa tcatggccaa ggcagcgaca ccacagtcac gctgatcaac ctgtgaaata
     7081 tatggatatt ttgacatggt aaccccttat taaagacaac ctgtgaaata aggcaagata
     7141 gtgataagta atgagaatag tgtaaataat tttagttttt tcaaaataaa actcctttta
     7201 actgttattt atgtttctat agtaaacatt taaccattaa aaaagtagtg acatttatgt
     7261 caccaccatt tctataaacc atctgccgct ttccctgctc ttatactttc ttctaaaacc
     7321 gagtcttcta aaacggaagc aaacattatg gccttatcgt aattctctgc agcctttttc
     7381 ttatttccct tatgcagaag ttcatattta gctttgagaa caaaaacact aggtttatag
     7441 ctatagaatt gtgatttttc tataacggct aatgctttat ccacaactgt taagaagtct
     7501 gaaaaggaat tgtgaaaaat cttcaaagca gcgatagcca tcaaatcatt aaataaaaca
     7561 atattataga catcatctgt ccaaatttcc tgatttaata ctctctttac gattctttca
     7621 aaaagttttt tatcatactg ttttcttcca taaagatgga agaaatagta gtcaataact
     7681 aataaatcgt tagttgagaa tttcctcttc cttttgactt gttcaaataa gtcttcatat
     7741 atctcctcaa ctttaggact ctcttcccaa ctagtaaaac tcaaaatatt ttccaaaata
     7801 tctagagtta acaattcttc ttctgggaga agttcaaaga atttctcata aacctcctca
     7861 attaaggcaa gtttagcctt tattctttct ggatttctat acgttggaaa cttaatcaga
     7921 cgattcttca tctcgtagta ttcgtcaggg atagtaatat tatcatgatc caataaatcg
     7981 gtcatatttt ttcctagacg ttttgcaata taagataatt tatccaaaga cgggagcgac
     8041 tccccttttt caatgcgaat taactgacga acagtcaatt ccgcaccatc taaacatagc
     8101 acttcacgtg acatctgctg acattcacgc tcagttttga ttcttagtcc aatgctgtct
     8161 tttatgttca agagactctc ctttatttca aaattttaaa aacattatat cataattgta
     8221 atataattaa tatatagtta attcattttt aacagaataa tagtgaaaat aagctatttt
     8281 tatacatttt atataggaag gaccttcctt tgtatcaaaa aacatgcagt tcaaaactac
     8341 atgttttttt atttctgttt actttggaat cgccattcaa agcgaatgcg gtcataagtt
     8401 ggataaatcg cattaagcaa cccaaaggct agggtaaagc cagctaaaac atctgttgga
     8461 tagtgaacac ccacataaat acgcgaaagt ccaacaagga aaatcagaag tccagtaata
     8521 ccataaaccg cagttttagc tgctttttta actagacgtt gggtaagaac aatagcaata
     8581 gcaccaagaa tcataaaagt ccccaaggag tgaccactag ggaaagaata gccaccagca
     8641 tgaaccaaat gggtgattga tgggcgtggg cgctggtaga tatacttcaa actcataatc
     8701 atagtgctag ctaagacgcc actagtccct acaaagatag cttctgcttt atactgacgt
     8761 agataaagcc aaatgaccgc taggattgca ataacagctt gaaccgacgt atttccaagt
     8821 gttgtaatca ttttaaagaa ggctgttagg cctccaggca aatctcctcg aacccaagtt
     8881 tgaatgttgc tatcaaatcc tgtcaaggct tcagggtaga acttaaccat ataaccgaga
     8941 gcaacaaata ataaaaaggc aaaagaagca taaatataat gtgtgtgttt gtgtttcata
     9001 tttataattt tactagaata gacttaagcg gagcataaat aagagcaaag gcaatcgaaa
     9061 aaatcaaacc ttcaagcaaa ttaaatggga caaccattgc aaaaaggtat ttatagagac
     9121 ctatgaattg tcctatatca aaattagcga atttggcata gagtggtact gcataaatat
     9181 agttaagtgc taccatagca actgtaagca agacagtacc ccaaacagat ccaagaatat
     9241 aattcttact cgtttgattc tttttccaaa aatagcttag gccccaaaca aagacgccta
     9301 gagcaacgaa attcatagga agtccaatca ttgatcctgg accaccattg tttagcaaaa
     9361 gcttcaacaa tgaacgaagc aagagaacac caatggcact cttaaaatca aagataacca
     9421 aggcgataag aactggaagg attgaaaaat caaccttcaa aaaatctgct gctggaatca
     9481 atggaaatga aaaataaagc aataagaatg aaactgcgga taaaatggca atataagcca
     9541 atttacgtgt gtttgtcata aaaaaattcc tccaaatttt aacaatttga aagaagttcc
     9601 caatgtattt gcctatgtca aaaaagacta cgcaaaaaga ctggtcttct tccatccaga
     9661 ctgtactgtc ggttgaggaa tcgcacctca tcagcttgcg ctcgcggact tatccataga
     9721 atcaccgccg gtcgggaatt tcaccctgcc ctgaagaccc tttaagatta acacaaaaaa
     9781 gccttgatga caaggctttc gatttataga tttctaatca aatgatagtc aacgactatt
     9841 aatagtctct attttaattt atcattctgg taacgatggc tcaactctaa ccccgcaaac
     9901 tgttgctgac gaagtgcctc ataaacaatc atacaaacag tattggacac atttagacta
     9961 cgcacatgtt catcattcat cggaatacga atagcttttt caggatgctc acgcataaaa
    10021 tcttcaggta ggcctgtatc ctcacgacca aacataaaat agtggtcctc accatcagca
    10081 taggcttctt ctgaataagt cttatccgca aacttggtga ctagatgcaa acgaccatct
    10141 tgatttttgg acatgaaatc tgccaaattg tcataataag tgatgtcaag cttatcccag
    10201 tagtctaaac ctgcacgttt cattttcttg tcatcaattg gaaagcccat aggacggatg
    10261 atatgaagag ggctattagt cgctgcacag gtacgtgcta tatttccggt gttttgtgga
    10321 atacgaggtt caaacaagac aatatgatta cgtgacatct caattctcct agacataaaa
    10381 aataccatgt cgcccggagt caagctcagc agacgacatg gttgtggacg ttcattagct
    10441 taaatcatga caggttagat ttaaaataac gaatctttga atatatttta gcacttctgg
    10501 tctaaaagtc aagactattt aaagaaaaat ttcaccttta ttcacttaaa taggaatagt
    10561 tgataaagct agctgaaaag gatgattttt ccgctaaaat agagtatcat agtaatgatt
    10621 atttggaggt aactcatgcg tattattgtc gtaggtgccg gtaaagtcgg taccgcactc
    10681 tgtcgttctc ttgttgaaga gaagcacgat gttattttaa ttgaagaaaa ggaagaggtc
    10741 ctcaaacgcc tcagcaaacg ttatgatgtt atgggctttg ctggaaatgg ggctaatttt
    10801 aaaattctgg aacaagccga ggttaacaac tgcgatgtct ttatcgcgat gactgataaa
    10861 gatgaagtaa acatgatttc agccgttctg gctaaacaaa tgggtgccaa ggaaactatt
    10921 gttcgcgtgc gtaacccaga atactctaat gcttatttca aagacaaaaa ctttcttgga
    10981 ttctcattag tagttaatcc tgaactttta accgctcgct atattgccaa tacggttgaa
    11041 tttccaaatg ctctatcagt agaaaccttt gctaacggac gcgttattct gatggcattt
    11101 aaagtcactg aaaatagcca attgtctggt atgacaatgg aacaattccg taggaaattt
    11161 ggcaatattc tagtttgtac gattgaccgt cagggtgaac tcatcattcc agatggaaat
    11221 gctaccatgc aaattggaga taagatttat gtcactggta aacgtgttga tatggcactt
    11281 ttccacagtt acatcaagcc aaaaagcatc aaaaaacttt tgttgattgg tgctggacgt
    11341 atctcctact acttgctcaa tatcttgaaa aacaatcgta ttaaggtcaa attgattgag
    11401 caaaaaatgg accgtgcgca aacctttagt caggtcttcc ctgaagtttc tgtgatctta
    11461 ggtgacggta ctgctaaaga ccttcttctc gaggaaagtg cagccagtta tgatgccgta
    11521 gctaccctaa ccggagtaga tgaggaaaat atcattacct ctatgttcct cgaaagcctt
    11581 ggtattcaca agaatattac taaggttaac cgtactagtc tcttagagat tatcgatcca
    11641 cgtgagacaa caacaattgt cactcctaag agtatcgctg ttgacactgt aatgcacttt
    11701 gtacgcgggc gttttaatgc caaagactct agtcttgatg cccttcacca tgtggctaat
    11761 ggtcgtatcg aaaccttgca attccaaatt aaggaaaata acaaaatcgc cggaaagaaa
    11821 ctttctgagc ttgaacttgc taacggtgtc cttatcgctg ctattatccg caatggaaaa
    11881 tctattttcc caacaggaga agacaggctc gctatcgggg ataaaatcgt ggttgttaca
    11941 ctcttacaaa atgtaactca catttacgac ttgttgaaga ggtaagctgc catgaatcgt
    12001 tctattgtcc gttttcttat tgccaagctc ctgctgattg aggctgctct cttggttgtg
    12061 ccacttatcg tcgctcttat ttatcaagaa aatgcta
//
