LOCUS       NZ_JADPFA010000004     12157 bp    DNA     linear   CON 28-JAN-2024
DEFINITION  Streptococcus salivarius strain D43t1_170807_H4
            NODE_4_length_215930_cov_33.4937, whole genome shotgun sequence.
ACCESSION   NZ_JADPFA010000004
VERSION     NZ_JADPFA010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus salivarius
  ORGANISM  Streptococcus salivarius
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 215930)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Quantification of discrete gut bacterial strains following fecal
            transplantation for recurrent Clostridioides difficile infection
            demonstrates long-term stable engraftment in non-relapsing
            recipients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 215930)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, 1470 Madison Ave, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 27.95x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 10:18:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,053
            CDSs (total)                      :: 2,002
            Genes (coding)                    :: 1,964
            CDSs (with protein)               :: 1,964
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 13 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 9 of 38
            Pseudo Genes (multiple problems)  :: 12 of 38
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:50:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 36519
            Orig. end                         :: 48676
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADPFA010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12157
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12157
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12157
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7157](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7157)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            207..1019
                     /locus_tag="I4U27_RS06425"
     CDS             207..1019
                     /EC_number="3.1.3.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1179: hydrolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /locus_tag="I4U27_RS06425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="WP_014633589.1"
                     /transl_table=11
                     /translation="MTKKLIALDLDGTLLRPDGTISEFTQKTIKDVQNKGHQVVIATGR
                     PYRMAIDHYKTLGLETPLITFNGSLTNLPDQDWAYEHSVKLDKKYLISLLQRHDELEMD
                     FLASEYRKHFYITMNHPERIQPQLFGVDKIIEAMRLEPTKITRNPNALLMQTRHEDKYQ
                     LADDIKKDFKEEIEVDSWGGPLNILEFSPKGINKAYALNYLLKAMRKSTDDLIAFGDEH
                     NDTEMLELAKTGYAMKNASSVLLPHANEQIEWTNEEDGVARKLQELFL"
     gene            1754..2491
                     /locus_tag="I4U27_RS06430"
     CDS             1754..2491
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013991119.1"
                     /locus_tag="I4U27_RS06430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_002883766.1"
                     /transl_table=11
                     /translation="MDIYILEDNIIQQGRLEQALDKALRSYGETLDCVQTFEQSNQLLE
                     YIKPANHQQLFFLDIEIKGKEKEGLHLGQTIRNKLPDAHIVFVTSHSEFMPLTFSYQLF
                     ALDYIDKNLDEDSFQSKVAIALSYVAKQSIKKEEKFSFHSGTDHIDLSYDRLLYIETSA
                     KRHYVILHGSHSIFEFRANISDLANANPKLFRCHRSYLINPDKLVRLNKTKREIEMSDG
                     TNCLVSRRHIKAVLAFMYEDKKI"
     gene            2761..3828
                     /locus_tag="I4U27_RS06435"
     CDS             2761..3828
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /locus_tag="I4U27_RS06435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_230311013.1"
                     /transl_table=11
                     /translation="MTITNLSLRMIAFFLYPLFNIGNDFFKRDIVFSNMSMIIPTLLSF
                     LLIGFFKQSFKFLKNQRPNTKSQRTIKTTNFLLIIYFIIQHSLVYVEEFTHSSTLDLRK
                     FIVIIYTILFFLMLIFLDRTTRDIMQEELNLQNNIRLKNLDTYNHKIEELYQTVRGFRH
                     NYRNLLTTLKLGIDQEDMTIVKDIYTSILETSDKRLETKQFDLTQLINIQDNTLKSLLS
                     AKFLQADDQNIDISLEIPSPITLEGMTILDFITVISILIDNAMEATVKAKKTKIMIAYI
                     KQDKYQRFIIKNSTKEESISITKIFQKGFSSKGTDRGIGLASIREILEYYPNVSLNTRS
                     VAYEFTQDLIIRQKT"
     gene            complement(3916..4983)
                     /locus_tag="I4U27_RS06440"
     CDS             complement(3916..4983)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009570339.1"
                     /locus_tag="I4U27_RS06440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_037610607.1"
                     /transl_table=11
                     /translation="MNPNLFKSTEFYHRRYHNFTTLLVFPLVLFVVFLVSFSFWAKKEI
                     VVQAIGTVEATRIIALIQSTSDNPIMANYLTSNKVVKKGETLIQYTETTEISQKDNLQQ
                     KLDLLKRQETQLKILKSSLEQGSNLFQEDDDEFGYQSTFSTYVSQLDELETSVQNNDGG
                     FVTDDTSISSKKLALKNQFLQEVVQQLSTLQSQIIDLEGKVNQAGFQLSSTQIQAPEDG
                     ILHIVQPAKQSTVVATGTELAQLYPNILDTREVLISYYVSSEYVSNLKKGQVVRLFLDK
                     VGNHGVTIKGSIESIDGFATETEKGNVFRITAKAKLTTKEAQNIKYGLQGHVTSVIAKK
                     TYFDYVKDKVLNQLN"
     gene            complement(5001..7157)
                     /locus_tag="I4U27_RS06445"
     CDS             complement(5001..7157)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062135.1"
                     /locus_tag="I4U27_RS06445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_037610609.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.3e-44, bitscore:
                     143.6, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1107.7,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRFRKTFVPQVDMMDCGVAALASISRYYGSDYSLAHLRELTKTT
                     KEGTTALGLVQAAKKMEFETRAIQADMSLFEIKDISYPFIVHVAKDGRLQHYYVVYKNK
                     KNHLIIGDPDPSVRITRMSKKGFEKEWTGVALFFAPEPSYKPYKDKKNGLLALVPLIFK
                     QRSLITNIVLASFLVTLINILGSYYLQVILDEYIPNQMQSTLGIISIGLVVTYILQQMM
                     NFAREYLLTVLSQRLTIDVVLSYIRHIFELPMSFFATRQTGEVTSRFSDANSIIDALAS
                     TILSVFLDVSILMIVGGVLLLQNRNLFWLVLYSIPIYLIIVYTFMKPFEKMNHDVMQSN
                     AMVSSAIIEDINGIETIKSLTSEEVRYQKIDSDFVGYLDNSFKLSRLSILQTSIKQGAQ
                     LILNVLILWTGAQLVMANTISVGQLITFNMLLSYFTTPLENIINLQTKLQSAKVANARL
                     NEVYLVDSEFQTFQENPYQEQLLKGDITVSDLSYKYGFGRDALSDINLTIKQGEKVSFV
                     GVSGSGKTTIAKLLVNFYKPYKGTISLNGQNIDQLDKRSLRQFINYLPQQSYVFSGSLL
                     DNLTLGADPSVVQEDILKACDIAEIRQDIEQMPMGFQTELSDGAGLSGGQKQRIALARA
                     LLTKSRVLILDEATSGLDVLTEKKVVDNLLQLTDKTIIFIAHRLSIAQRTHRIFVMDQG
                     RIVESGSHEELLSQAGFYQRLFQS"
     gene            complement(7630..7851)
                     /locus_tag="I4U27_RS06450"
     CDS             complement(7630..7851)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623337.1"
                     /locus_tag="I4U27_RS06450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="garvicin Q family class II bacteriocin"
                     /protein_id="WP_022495973.1"
                     /transl_table=11
                     /translation="MAAQTLNNFNSLDSEALSTVGGGRGICRYVWSGANGYSCRYPDGR
                     WDYIVTKSPSQAVYDIAVHGWSGFYIAP"
     gene            complement(8217..9179)
                     /locus_tag="I4U27_RS06455"
     CDS             complement(8217..9179)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744512.1"
                     /locus_tag="I4U27_RS06455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thioredoxin family protein"
                     /protein_id="WP_002883806.1"
                     /transl_table=11
                     /translation="MAQKRLKTLLTSSLLLTSLLASVTLADELIDTEYNSTSPVEVVDT
                     TMTNQIATSSEKDVGIATTSQEEPKQQVDTSVSDPDSPIGVALPSLGESEEETADKIPT
                     VTVDEYKHNVTELPKITIEDVHHSFTEDNQKHTIYFGRETCYYCRQFSPELKVLNQLLE
                     GRLEYYDTDQPDFDRSYIFGEIGIPGTPTLLYLENGKVLSGWVGGGSAKEVYDYLSQSS
                     QHFMTSSNAPTEQTSIAIQPYVTEEENKKVGQLHQSEALSSVSVSTQTSKFVQKSEASL
                     PTLGTSDDTVIIILGLVSSAVAFWIIGSKTYNNKEKSLK"
     gene            complement(9945..10073)
                     /locus_tag="I4U27_RS10330"
     CDS             complement(9945..10073)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I4U27_RS10330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_002883737.1"
                     /transl_table=11
                     /translation="MIKSKILIADTTYTKAHYNHKKLQEVFRERSKALHKTIYQCS"
     gene            complement(10092..10301)
                     /locus_tag="I4U27_RS10235"
     CDS             complement(10092..10301)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /locus_tag="I4U27_RS10235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_254388426.1"
                     /transl_table=11
                     /translation="MMFKYLLLKDIYKLSDVEVLEQSFSDMAFKYFLALSPEDSVIEPS
                     SLTKFRKLRIKDERLLDLLIVKSI"
     gene            complement(10550..11281)
                     /locus_tag="I4U27_RS06465"
     CDS             complement(10550..11281)
                     /EC_number="3.5.-.-"
                     /GO_function="GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713635.1"
                     /locus_tag="I4U27_RS06465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cyclase family protein"
                     /protein_id="WP_002883746.1"
                     /transl_table=11
                     /translation="MSDLLSIYNELQSKKWVDLTHKINAESPHFPALPALKQEDLFTLK
                     DGFHVQKFTVVGQYGTHIDAPIHFVEGGRWLDEIALEDLLLPLYVINKSKEVAANPNFI
                     LSKQDILDFEAEHGKITEGAFVAFRSDWSKRWPDQDAMRNLDENGVQQSPGWSREALEF
                     LIHERHVKAVGHETFDTDAGIPAAEHGLVNEYYLLEQNIYQVEVLNQLDQVPAVGALIS
                     IAFPHWDKATGSPVRAVAILP"
     misc_feature    complement(11431..11445)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.16"
                     /tool="antismash"
ORIGIN
        1 aagtttagac tccttaataa aaaatattgt caaaatccga ataatttcgg aacattctca
       61 tgtagaatgc aacattacta ttctacatga aatcatttga cggtgcaagg tttatttcct
      121 gagacttttc ttatgaaaaa ccgaacgttc ttagaaagct ctttcttaat caccaacaaa
      181 ggacagattt ttgctataat aggaacatga caaaaaaatt gattgcacta gatcttgatg
      241 gaacgttatt acgcccagat ggaactattt cagaatttac acaaaaaacg attaaagacg
      301 tacaaaacaa aggacaccaa gtcgttattg ctacaggtcg tccttatcgt atggctattg
      361 accattacaa aacactaggg ttagaaacac cactcatcac cttcaacggc tctttgacca
      421 atcttccaga ccaagactgg gcttatgagc attcggttaa attagataag aaatatctga
      481 ttagcctctt acaacgccat gatgaattag agatggattt tctggctagt gaatatcgaa
      541 aacacttcta tatcactatg aatcacccag agcgtattca accacaactc tttggtgttg
      601 ataaaattat tgaggctatg cgtttagaac caactaaaat cacgcgcaac cctaacgctc
      661 tccttatgca aactcgtcat gaggataagt accaacttgc cgatgacatc aagaaggact
      721 tcaaggaaga aattgaagtt gattcttggg gtggcccgct taatatcttg gaattttcgc
      781 ctaaaggcat aaacaaggcc tacgccctaa actacttact taaggccatg cgcaaatcaa
      841 cggatgatct gattgctttt ggagacgaac acaacgacac agagatgttg gaacttgcta
      901 aaactggtta tgccatgaag aatgctagct ctgtccttct cccccacgct aacgaacaaa
      961 ttgagtggac taatgaagag gatggcgttg ctcgcaaact ccaggaactc tttttataaa
     1021 taacaaaagg catcagagat ttccgagcca ctgaaaaaat catcgctata actcaaataa
     1081 aataataaag ggaggaatct atcttatatt ttggtagatt cctccttttt accaataact
     1141 ttcacaatag tggcttcctc gtgaatgatg aaatccttct tcggtataat tatccttcgg
     1201 agatgagcga aatcatcacc tactgtatta aatagagaaa gtgtattaaa tagagaaagt
     1261 gtattaaata gagaaagcca tttacaatta tattttgttc caccaccatc aacgattcaa
     1321 aactaagaga acctagcctt atgcagtaca gaattaatcc agttttctaa tgctttttgg
     1381 ctaaccatct tgaatcagta caatcttaaa catataatat tcaaggtaat gaataaaaaa
     1441 gaactttcta caattgggtg aacactttat caaaatttcc tttattttac gacgcaatat
     1501 cataccgaga ccaactaata tcatcaaagc aacaatagct gtaactagaa gctttaaaaa
     1561 gataacgtaa tatattataa gtattttgaa tcagctcagc aaaataaatt actcagttta
     1621 aacttgaaaa agcgctcata aacatagctt agctctcatt taattgcaat tagattataa
     1681 ttatattata ttttatgtga ttcttttaat tttattcgta aaaagaatat ataacacaat
     1741 aaaggactaa aatatggata tctacatttt agaggacaac ataatacagc aaggtagatt
     1801 ggaacaggct ctggataagg ctctcagatc ctatggtgag acactagact gtgtccagac
     1861 ctttgaacaa tccaaccaac ttctggaata tataaagcca gccaaccatc aacaactctt
     1921 tttcttggat attgagatta aagggaaaga aaaagaagga ctccatttgg gacaaaccat
     1981 ccgaaacaaa ctaccagatg ctcatattgt ctttgtaacc tcccactcag agtttatgcc
     2041 cttaactttt agttaccaac tctttgccct agactatatc gacaaaaatc tagatgaaga
     2101 ttcttttcaa tcaaaggtcg cgatagccct atcctatgtc gctaagcaaa gtataaaaaa
     2161 agaggaaaag ttctcttttc attctggaac cgaccatatc gatctctcct atgaccgttt
     2221 gctttatatt gaaacatctg ctaaaaggca ctatgttatc ttacacggtt cccatagcat
     2281 ttttgagttt cgagccaata tctcagactt agctaatgcc aatcctaagc tcttccgctg
     2341 tcatcgctcc tacctaatca accctgacaa gttggtacgt ctcaataaga ccaaacgcga
     2401 aatcgaaatg tcagacggca ctaactgtct ggtatctcgt agacatatca aggcagtctt
     2461 agcctttatg tatgaggata agaagatatg atgttaaaac tagttttttt gtttcttata
     2521 aattttagca tagggataga actagcactc ttcctatatc tcattgatcg aaaagtgtca
     2581 gttaggcaat ttctgttttt gctacttctt aaaccactat ttaattttat ttgtaatctg
     2641 acgagatggg gtattctacc ttactttagt gaagaaatat tttatttttc cctttctttt
     2701 ttactattaa gaaagtctac cctttcgtct gaaataaagt tattttatag catattcttt
     2761 ttgacaataa ctaatctcag cttaagaatg atagctttct ttttataccc actatttaat
     2821 attggtaatg acttttttaa aagagatatc gttttttcta atatgtcaat gattattcct
     2881 acattgctgt cttttctttt aattggattc ttcaaacaaa gtttcaaatt tttaaaaaac
     2941 cagcggccta ataccaaatc ccaaagaact attaaaacta cgaatttctt attgataatt
     3001 tattttatca ttcagcactc cctagtctac gttgaggaat ttacccattc atctacacta
     3061 gacctacgca aatttattgt cattatctat accattttgt tcttcttaat gctcatcttt
     3121 cttgaccgaa ctactagaga tataatgcaa gaagaactaa atctacaaaa caatatcagg
     3181 ctaaaaaatc tcgataccta caatcataaa attgaagagc tttatcaaac cgtccgtggt
     3241 tttcgtcata attatagaaa cctacttacc actctgaaac taggcattga tcaggaagac
     3301 atgacaatag tcaaggatat ctacacttcg attttggaaa catctgataa aagactagaa
     3361 accaagcaat ttgacttgac acaactaatc aatatccaag ataatactct aaaaagcctc
     3421 ctatctgcca aatttctcca agcagatgac caaaatattg atatctccct agaaattcca
     3481 agtcctatca cgttagaggg aatgactatt ctggacttta tcactgttat ctctatactg
     3541 atagacaatg ccatggaagc tactgtaaaa gctaaaaaaa ctaagataat gattgcttat
     3601 attaaacagg ataaatatca acgcttcatt atcaaaaata gtaccaaaga agagagtatc
     3661 tctattacca aaatctttca aaaaggtttc tcaagcaaag gaactgaccg tggtattggt
     3721 ctagctagta taagagagat actagagtat tatcccaacg tttctcttaa tactagaagt
     3781 gtggcatatg agtttactca ggacctaatt ataagacaaa aaacttgatc tagcgtcaag
     3841 ttttttgtct tataaatctt tcaacggata cttattcatg ttttcttgtc aatccttgag
     3901 tttttacttc ttggtttaat ttagttgatt aagcacctta tctttgacat aatcaaagta
     3961 agtcttttta gcaatgacgc tcgtaacgtg tccttgcaaa ccatacttga tgttttgagc
     4021 ctccttagtc gttaactttg ctttagctgt gatacgaaaa acatttccct tttcggtttc
     4081 agtcgcaaaa ccgtcaatgc tttctatact tcctttaatt gttacaccat gattgccaac
     4141 cttatcgagg aatagtcgaa ccacttgccc tttcttgaga ttagaaacat attctgagct
     4201 gacatagtaa ctgatcaaca cctccctagt atccaaaata ttaggataaa gttgggctaa
     4261 ttctgtccct gtggcaacta ctgtagattg tttagccggt tgaacaatat ggaggatgcc
     4321 atcttctggt gcttggattt gcgtacttga caattgaaag cctgcttgat tcactttacc
     4381 ttctaaatca ataatctgtg attgtaaagt ggatagttgt tggacgactt cttgcaaaaa
     4441 ttgatttttt aaggccagtt ttttagaaga tatactagta tcatctgtaa caaatcctcc
     4501 atcattattc tgaaccgatg tttctagttc gtccaactga ctaacatagg tactaaacgt
     4561 actttggtag ccaaattcat catcatcttc ttgaaaaagg ttagaacctt gttcaagact
     4621 actctttaaa attttcaact gtgtctcctg tcgctttagt aaatccagtt tctgttgaag
     4681 gttgtctttc tgtgaaatct cagttgtctc cgtgtactga atcaaagtct cacctttttt
     4741 gaccacttta ttggatgtca aataattagc cataatagga ttatcactgg ttgactgaat
     4801 cagcgcaata atacgtgttg cttctaccgt acctatggct tgaacaacaa tctctttttt
     4861 agcccaaaag gaaaaggata ccagaaaaac cacgaataag actaaaggaa aaacaagaag
     4921 cgtcgtaaaa ttatgatacc gcctgtggta aaattctgtg cttttaaata aattcggatt
     4981 catactgttt ttcctcccga ttaactttga aagagacgct ggtaaaagcc tgcttgagac
     5041 aacaattcct catgagaacc actctcaact atgcgacctt gatccataac aaaaatacga
     5101 tgtgttcgct gagcgatact caaacgatgg gctataaaaa ttatcgtctt atctgttagt
     5161 tgcaataagt tatcaacgac ttttttctct gttaatacat ccaatccact cgtcgcttca
     5221 tctaagataa gcactcgaga tttcgttagg agggcacgag ccaaagcaat cctctgcttt
     5281 tgtcctcctg aaagacccgc tccatctgaa agttctgttt gaaatcccat gggcatttgt
     5341 tcaatatctt gacgtatctc tgctatatcg caagctttta aaatatcctc ctgcaccacg
     5401 ctaggatcag ctcccaaagt taggttgtct aatagactac cactaaaaac ataagactgt
     5461 tggggaagat agttgataaa ctgacgtaag gatctcttat ccaactgatc gatattttga
     5521 ccatttaaac taatcgtacc tttgtaaggc ttataaaagt ttactaagag cttggctatg
     5581 gttgtcttac cagacccact aacacccaca aaactaactt tttctccttg tttgatagtt
     5641 agattgatat ctgataaagc atctcgacca aaaccatatt tataagatag gtcagagaca
     5701 gtgatatcac ctttaagtag ctgttcttga tatggatttt cttggaacgt ttgaaactct
     5761 gaatccacta gatatacctc gtttaaacgg gcattggcca cctttgcaga ctgaagtttg
     5821 gtttggagat tgatgatatt ttctaatggt gttgtgaaat aggacaagag catgttaaac
     5881 gtaatcaact gaccaactga tatcgtattt gccataacga gctgagctcc tgtccataga
     5941 atgagcacat ttaaaatcaa ttgtgcccct tgttttatag aggtctgtaa gatagataat
     6001 ctggacaact taaaggagtt atccaaataa cctacaaagt cactatcaat cttttgataa
     6061 cggacctctt cactagttag agatttaatg gtctcaatcc cattgatatc ctcaattatc
     6121 gctgagctca ccatagcatt gctttgcata acatcatggt tcatcttttc aaagggcttc
     6181 ataaaggtat agacaataat caaataaatc ggtatggagt acaagactaa ccaaaataga
     6241 ttcctatttt ggagaagaag gacaccaccc acaatcatca aaattgaaac atctaagaaa
     6301 acagataaga tggtcgatgc taacgcatca ataatcgaat tggcatcact aaaacgtgaa
     6361 gttacctctc ccgtctgtcg cgtcgcaaaa aaggacatgg gaagttcaaa aatatgccga
     6421 atataggata agaccacatc aatagttaga cgttgactca gcacagtcag taagtactcc
     6481 ctggcaaaat tcatcatttg ttgcaaaatg taggtcacaa ccaaaccaat tgaaataatt
     6541 cccaaagttg actgcatctg attcgggata tactcatcca aaattacctg caagtaataa
     6601 gaacctaata tattgatcaa agtaactaaa aaactagcta agacaatatt ggtgataaga
     6661 gatctttgct tgaaaataag tggaacaaga gctaataaac catttttctt atccttgtat
     6721 ggtttatagc tgggttctgg tgcaaaaaat aaggcgacac ctgtccactc cttttcaaac
     6781 ccctttttgg acatcctagt tattctgacg ctagggtccg gatctccaat aatgagatga
     6841 tttttcttat ttttatagac cacataataa tgttgtagtc taccatcttt tgcgacatga
     6901 acgataaaag gataggatat atctttaatc tcaaagagag acatatccgc ttgaatagcc
     6961 cttgtttcaa actccatttt tttagccgcc tgaactaaac caagggctgt tgtgccctcc
     7021 ttggttgttt tggttaattc tctcaaatga gccaaggagt agtctgatcc ataataccta
     7081 gagatagaag caagagctgc cacaccacaa tccatcatat caacctgagg cacaaaagtt
     7141 ttacgaaaac gtaacattac atctcctttc ctatatttct ttcacactcc tcagtataac
     7201 agccctcata attaatagtt gatcctgtca caaatggtcc aaatatggca cgaatggtga
     7261 aaaatcagac tataattata aaagaccaac taaatctacc ttaataggtt aaattcagtt
     7321 gatctttttt gtaattttca ttactaatca tcacaaacat ataaattatc aaaaagacaa
     7381 gtcaattgat gataatcctc ttgataaatt aattttacgt tttgattcat cttgtaatgt
     7441 cgtaagagta ttactcaact cactcctgac ctcagtgata ttcctttaaa aagattaata
     7501 actgctagaa tttgaataca tctccgttct tttgcagttg tttatcaatt tagaagtaag
     7561 attatagata ttatttatga tattcgcaat tgggctgtta ttgctctttt tcaactagat
     7621 tactttctat caaggggcaa tgtaaaatcc actccaacca tgaactgcaa tatcataaac
     7681 cgcttgagaa ggagactttg ttacaatata atcccaacgt ccatctggat aacggcaaga
     7741 atatccgttt gcaccagacc aaacatagcg acatatacct cttccacctc caacagtaga
     7801 gagagcttcg gaatcaagtg agttgaaatt gttcagtgtt tgtgctgcca taagcgacaa
     7861 cctcctttaa atagtttttt gtgatatcat tatcacatgt ctagtataac aagttcaatc
     7921 ccaaaaagat ctttctgtca caaatggtcc atttatggca cgaacagaca aaagaaggcc
     7981 atttgccatt gaccaatctg ttataaaatg attatttaag cgtatcacat gatacactca
     8041 aataatcatt ttataggtcg ctacatgtta tagcagtagg tctttgaacc attaaagttt
     8101 caccctattt tttctgtttt tgaaatggtc tcccaaaggt ccaaccatcc agcggagccg
     8161 acactagtgg attagcagaa actaagatac agtgtcttgc ttagtaaaat cgattatcat
     8221 tttaatgact tctccttatt attatatgtc ttactaccta taatccaaaa agctactgct
     8281 gagcttacaa gacccaaaat aattataaca gtatcatctg aagtgcccaa tgtaggtaga
     8341 gatgcctcag atttctgaac aaacttagaa gtctgagtag aaacggaaac cgatgacaaa
     8401 gcttcagatt ggtgtaattg ccctactttt ttattttctt cctctgttac atatggctgt
     8461 atagcaattg atgtttgttc cgtaggagca ttactacttg tcataaagtg ttggcttgat
     8521 tgcgaaagat agtcataaac ttccttagct gaacctccac caacccaacc tgaaagaact
     8581 tttccattct ctaggtacag aagagttggt gtccctggaa taccaatctc accgaaaata
     8641 tagctcctgt caaaatctgg ttggtcggtg tcatagtatt ctaaacgccc ctctaacaat
     8701 tgattcaaca cttttaattc aggtgagaac tgacggcagt aataacatgt ttcacggcca
     8761 aaataaatag tatgtttctg gttatcctct gtaaaagaat gatgtacatc ttctattgtt
     8821 attttcggca actcagttac attgtgctta tattcgtcaa cagtaactgt cggtatctta
     8881 tccgcagtct cctcctcaga ctctcctaag gaaggtaatg ctactccaat aggagagtct
     8941 ggatcagaaa cactagtatc aacttgttgt tttggttcct cctgactagt cgttgctata
     9001 cctacatctt tctccgatga ggtggcaatc tgattagtca ttgtcgtatc tacgacttca
     9061 accggtgaag tcgaattata ctcagtatct attagctcat cagccaatgt cacactagcc
     9121 aataaactag tgagtaatag cgaactggtc aataatgttt ttagtctttt ttgtgccata
     9181 ataactcctt atttctgata gttttattat aataaccaaa taaacttgat tttcttcctg
     9241 gcacaaatga ccagaatatg gcacaaatgg ctccctcctc aacaacttag tttactaatt
     9301 ctgattatac aaatatgtaa aaacatgacc agtcgtcacg ttttttgtat aacatacttc
     9361 attttaacag tctcaccttt gctctacttg tcttagtcgt tttaattggt gagaacattg
     9421 tttacctaat aatatttcaa tgacttttaa cgtattttct caccttatgg gtagtcatta
     9481 catcactaaa cagtaataaa accataaaat caaataggtg ggatggtttg taatatccta
     9541 tatggttact atcttgaaga tgataacaca aaaggtagat tttttctgtc taacttctgg
     9601 ggtgcagttc actagccaca ccaagatcat atctgtattt aagttcctca ttttccctat
     9661 cttaaagcaa tgtttgtcct tttctttaaa ttctggaatc tcttctgaaa gtggtattca
     9721 ttactattaa gggtaataga ataggttttt gacttagccc cttctgtata ataccccact
     9781 ttttaaagga cagattttac aatacttgat atcaaaataa tagatttcct tagaattata
     9841 tctcaccatt attcttcctg tcctgacttt ctttagctcc atatgtcctt ctggataagc
     9901 aaagagatct acatccttgt tataaaaaat tcttctttga tatcttatga gcattggtaa
     9961 attgttttat gcaaggcttt tgaacgttca cgaaaaacct cttggagttt cttatgattg
    10021 tagtgagctt tagtataggt cgtatccgca atgagaattt tacttttaat caagccatgt
    10081 tccagagcaa tttatataga tttaacgatg agtaaatcga gtaaccgttc atctttgatt
    10141 ctaagtttac gaaatttagt caatgaagaa ggttcaataa cgctatcctc tggagacaga
    10201 gcaaggaagt atttaaaagc catatcagaa aaggactgct ctagcacctc cacatcggat
    10261 aacttataga tgtcttttaa taagagatat ttgaacatca taatcggcga ataagcttta
    10321 cgtccaagcc aggacgataa tttttttcta actcatcgta aataaaggta aaatcacaga
    10381 gttccgttag ctgacgtaaa aaatgatttt ttggaacaac aatatcatct agactggact
    10441 aaagttaact ggttatcaag catacaatcc cttaagtttt gtataacaaa aaagtcatcg
    10501 attgatgact ttttcagtgc gattagattt ccggtatttt ttcttatgat taaggtaaaa
    10561 tagccactgc acgcactggg gaacctgtcg ctttgtccca gtgaggaaat gcaattgaaa
    10621 tcaaagcacc aacagctgga acttgatcca attgattcaa gacttccacc tgataaatgt
    10681 tttgttccaa gaggtagtat tcgttaacca agccatgttc tgctgctgga ataccagcat
    10741 ctgtatcaaa agtctcatga ccgacagcct taacatggcg ttcatgaatc aagaattcta
    10801 gagcttcgcg gctccaacct ggactttgct gaaccccatt ttcatcaagg ttgcgcatag
    10861 catcttgatc tggccaacgc ttcgaccaat cactacgaaa agcgacaaag gccccttcag
    10921 taatttttcc atgctctgct tcaaagtcta agatatcttg tttgctcaag ataaagtttg
    10981 gattcgcagc aacttcttta gacttgttga taacatagag tggtaaaaga agatcttcga
    11041 gggcaatctc atccaaccaa cgaccacctt ctacaaagtg aatcggtgca tcaatatgcg
    11101 ttccgtattg accaaccact gtaaattttt gaacatggaa accatctttt aatgtgaaaa
    11161 gatcttcttg tttcaaagca ggaagggctg ggaaatgtgg actttccgca ttaattttgt
    11221 gggtcaagtc aacccatttc ttagattgca attcattata aatacttagt aaatctgaca
    11281 taaggaggcc ctcttttctt atagatttaa ccttaaatag ttaattgaag aaattatagc
    11341 agccaacacc agctattaca agcttttatc tcattttttc ttaatcctat ttcctctagt
    11401 tagatatagc ttaaaactat atttaacact tattgtctga aaaaaaagta tatttgcaat
    11461 aatttgatgt ttttttcaat tatatgacac ttttgttacg aaaaccattg acaaattcat
    11521 ggtaacgtcc tataattaga gtgttctaat tttcgaggga ctaaaaaaat attgttgtca
    11581 tttcatactg atcgatgcta ataccaataa tcatttttct attcctaaga aacaatatat
    11641 aaaaggagga agaacactaa tgggtatcgg tattattatt gctagccatg gtaaattcgc
    11701 tgaaggtatt caccaatcag gttctatgat ttttggtgaa caagagaaag ttcaagtggt
    11761 tactttcatg ccaaatgaag gtcctgacga cttgtacgct cacttcaaag atgccattgc
    11821 tcaatttgac gctgacgacg aaattctagt ccttgcagac ctttggtctg gatcaccatt
    11881 taaccaggca agccgaatta aggaagaaaa tcctgatcgc aagatggtta tcgtgacagg
    11941 actcaactta ccaatgctta tccaagcata tacggaacgt atggtagctc ctgatgctgg
    12001 ggttgaagag attgtcgcta acatttataa ggaaacaaaa gaaggcgtta aagtccttcc
    12061 agaaggcctt attcctgaag aagacactaa accagcagac gctaaacctt ctattcctaa
    12121 gggtaccatt cctgaaggaa cagtacttgg tgatgga
//
