LOCUS       NZ_CP058255            17422 bp    DNA     circular CON 30-MAR-2024
DEFINITION  Priestia megaterium strain BHS1 chromosome, complete genome.
ACCESSION   NZ_CP058255
VERSION     NZ_CP058255.1
KEYWORDS    RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 5373548)
  AUTHORS   Vilchez,J.I., Tang,Q., Kaushal,R., Wang,W., Lv,S., He,D., Chu,Z.,
            Zhang,H., Liu,R. and Zhang,H.
  TITLE     Complete Genome Sequence of Bacillus megaterium Strain TG1-E1, a
            Plant Drought Tolerance-Enhancing Bacterium
  JOURNAL   Microbiol Resour Announc 7 (12), e00842-18 (2018)
   PUBMED   30533669
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373548)
  AUTHORS   Abdul Wahhab,B.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2020) Bioscience, UTM, Skudai, Johor, Johor 81300,
            Malaysia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: GS De Novo Assembler v. 2019-10-29
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 18.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015582655.1-RS_2024_03_29
            Annotation Date                   :: 03/29/2024 13:26:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,643
            CDSs (total)                      :: 5,510
            Genes (coding)                    :: 5,455
            CDSs (with protein)               :: 5,455
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 117
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 16 of 55
            Pseudo Genes (incomplete)         :: 30 of 55
            Pseudo Genes (internal stop)      :: 17 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 14:55:19
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4610875
            Orig. end                         :: 4628297
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP058255.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from microbiology Lab of UTM.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..17422
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="phosphonate"
                     /region_number="9"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     cand_cluster    1..17422
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..17422
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:12422]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..12422
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     gene            complement(13..246)
                     /locus_tag="HW369_RS23940"
     CDS             complement(13..246)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="HW369_RS23940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_176536144.1"
                     /transl_table=11
                     /translation="MIGGQDEDSCGKSGIGETGRSLATRRLSGRTRKAKSCTEINRDGT
                     SEPYELMYPIFSSLDWIHLVISQPLFYIDFKL"
     gene            complement(367..1323)
                     /locus_tag="HW369_RS23945"
     CDS             complement(367..1323)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081827.1"
                     /locus_tag="HW369_RS23945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LLM class oxidoreductase"
                     /protein_id="WP_195695306.1"
                     /transl_table=11
                     /translation="MTAFQHHKAYRRMYKENELTLGLHMPLENYQYATPTMEKQVELTQ
                     LSEQYGFTSLWFRDVLLQDPSFGDPATGQIYDMLIYLTYLASQTKEIALSTSAIVLPLR
                     HPLRVAKEMATIDQLFPERLIMGVSSGDRQADFRGLGIPHEERGKRFTEAFHYLEAALN
                     QDFPSIQSTLGSVQGANVVPKPTKRIPTMITGYSQQNMEWFAQYGDGWMYYPRHPHFQA
                     EAITQWRKLVDIYHPGVFKPFTQPMHLDLAEDPNEMPTPIRLGFRAGRNALIELLSIYK
                     EIGVNHLFFALFDSKRPAEEIIHELGEEVLPHFPAHL"
     gene            complement(1381..2580)
                     /locus_tag="HW369_RS23950"
     CDS             complement(1381..2580)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1184: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461498.1"
                     /locus_tag="HW369_RS23950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_028407533.1"
                     /transl_table=11
                     /translation="MNTLSQTQQSKKSGALPLLALAISAFGIGTTEFVPVGLLSSLADD
                     LNISITLAGLLISGYAMGVAFGAPVLTALTSKMSRKSLLMLLMVIFIVGNSVAALSTSF
                     GLLLIARIITAFSHGIFFSIGATIAADLVPEDKRASAIALMFTGLTVATVTGVPLGTFI
                     GQAFGWRATFWGVALLGVIAIIASALLVPKNLKEAPPAKFSDQLKILKNGPLLLSFAIT
                     ALGYGGTFVAYTYLAPILEKLTGYAPSAVSILLLVYGVAVAIGNTIGGKAANKDPLKAL
                     FWMFLIQAVILVILSFTASFKVAGTITIFAMGLLAFMNVPGLQVLVVNLAEKYVPSAVN
                     VASALNIAAFNVGIAIGSFVGGLIVDSIGLVHTPWIGGVMVLGAVLLTAWSRSIEAKAK
                     "
     gene            2910..3278
                     /locus_tag="HW369_RS23955"
     CDS             2910..3278
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461499.1"
                     /locus_tag="HW369_RS23955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_098315079.1"
                     /transl_table=11
                     /translation="MKKYNIPVEAALEVIGGKWKVVILCHLIKGTKRPGELKRIMPRVT
                     QKVLTQQLRELEADGIIVRKMYNEMPPRVEYSLTNYGWSLKSILDSLCSWGEHHIEKTY
                     PDKEAVLMQPVEEVTVSN"
     misc_feature    complement(3284..3299)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.77"
                     /tool="antismash"
     gene            3361..3540
                     /locus_tag="HW369_RS23960"
     CDS             3361..3540
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002015431.1"
                     /locus_tag="HW369_RS23960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_060744848.1"
                     /transl_table=11
                     /translation="MTEQSKKKISLQDAIKQQLANKKNQSSNKAKNSSTNQATKKLTSQ
                     QTKKTNNQRKRTGV"
     gene            3540..3737
                     /locus_tag="HW369_RS23965"
     CDS             3540..3737
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     IEA]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055498.1"
                     /locus_tag="HW369_RS23965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YwbE family protein"
                     /protein_id="WP_028407531.1"
                     /transl_table=11
                     /translation="MNGQNRKDVAPGIKVDIVLKADQRSGKLTHGTVKDILTNSSFHPH
                     GIKVRLTDGQVGRIKEIYPN"
     gene            4607..4771
                     /locus_tag="HW369_RS23970"
     CDS             4607..4771
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461503.1"
                     /locus_tag="HW369_RS23970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="WP_081731992.1"
                     /transl_table=11
                     /translation="MKQPQLEKEIRALQSDIYQLAKKTSSYSQGEILKLSQKLDQKIVS
                     YQKLFNHTK"
     misc_feature    4887..4901
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.86"
                     /tool="antismash"
     gene            5001..6677
                     /gene="alsS"
                     /locus_tag="HW369_RS23975"
     CDS             5001..6677
                     /EC_number="2.2.1.6"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0003984 - acetolactate synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="alsS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561828.1"
                     /locus_tag="HW369_RS23975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetolactate synthase AlsS"
                     /protein_id="WP_195697120.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 4.7e-51, bitscore:
                     171.1, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.2e-43, bitscore:
                     146.9, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNADTTETKKGADLLVESLASQNVPYIFGIPGAKIDGVFDVLKE
                     KGPEVIVCRHEQNAAFMAAAIGRLTGTPGVCLVTSGPGAANLATGLVTATSEGDPVVAL
                     AGAVPRADRLKKTHQSMDNEGLFKSIAKFSVEVEDAANVPEVITDAFREAVSSPSGASF
                     VSLPQDVMDSETSASTLSILKAPTIGTANLDAVAQAIHTIRTAKLPVLLLGMRASKSKA
                     VESIRQLIAEHDLPVVETFQGAGAVSRELETHFFGRVGLFRNQPGDVLLKHADVVLTIG
                     YDPVEYPPSLWNSESQRSIIHLDDLQAQADHHYQPEIELVGDIPSTIDILADKLPHLIR
                     SKESASFLKTLKSKLEQPDDQYEFSSDTHLHPLQLIEALQNFVEDDTTITCDVGSHYIW
                     MARYFRSYEPQHLLFSNGMQTLGIALPWAISAALVRPHKKVLSMSGDGGFLFSAMELET
                     AVRLNSPIVHIIWNDQTYDMVGFQQQIKYGRKSAVEFSSVDFVTLAESFGAKGFRVNSP
                     EEFPDTLASAFRERGPVIIDVPVDYKDNEKLNTQLLPDQIN"
     gene            6694..7407
                     /gene="budA"
                     /locus_tag="HW369_RS23980"
     CDS             6694..7407
                     /EC_number="4.1.1.5"
                     /GO_function="GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006113 - fermentation [Evidence IEA]"
                     /GO_process="GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="budA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016763108.1"
                     /locus_tag="HW369_RS23980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acetolactate decarboxylase"
                     /protein_id="WP_195695307.1"
                     /transl_table=11
                     /translation="MSNKEKLTIHQTSTMIALLEGVFDGVVTYKDLAKKGDFGIGTFNQ
                     LDGEMIAFDGEFYHIADGKAAPISKDAKTPFAIMTTFYEDISYRVEKEMNREQLEILMN
                     ELIPSPNLFYAVRIDGKFKEVKTRTVAKQEKPYPSMVDAAADQPTFTSSDVNGTLAGFY
                     TPEYSSGIGVPGYHLHFIDEYKKEGGHVLDVSVQDVTIQICKKTKLNLNLSETSEFLNA
                     NLEDHDVEEEIATAE"
     gene            complement(7518..7940)
                     /locus_tag="HW369_RS23985"
     CDS             complement(7518..7940)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461506.1"
                     /locus_tag="HW369_RS23985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX hydrolase"
                     /protein_id="WP_195695308.1"
                     /transl_table=11
                     /translation="MNQKLAVAVMVINQDHHVLLVKNHRRGWEFPGGFVEAGESIKDAG
                     IREVKEESGIVIEVTNFLGVEQDVKRSTTVILLKGKAISGEISVSSETQDVGYFTLEEA
                     KNIIKLDNFKDRMARCLKEAEIPFFYVLKDTGSFYV"
     gene            complement(7948..9789)
                     /locus_tag="HW369_RS23990"
     CDS             complement(7948..9789)
                     /EC_number="2.6.1.37"
                     /GO_function="GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_5 (266-535). E-value: 2.3e-29.
                     Score: 94.4. Matches aSDomain:
                     nrpspksdomains_HW369_RS23990_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055494.1"
                     /locus_tag="HW369_RS23990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2-aminoethylphosphonate aminotransferase"
                     /protein_id="WP_195695309.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 2.6e-11, bitscore:
                     42.5, seeds: 297, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 9.7e-24, bitscore:
                     82.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKTAVILAAGMGSRIRKRAGNRPKGFLMIDEKSIIEHSISRLIE
                     AGVKTIFIGTGYMKEEYEKLMVRYPQIKCVYNSRYETTGSLFTLYQFKNYIKEDFLLLE
                     SDVIYEKNALETLVNHSRSDVILASKLNGAGDEVYIEADENNNLKNMSKQKEELNSIYA
                     ELVGLTKLSYATFQRLCDEANTLFQFNQTIDYEYGLVKISEKANMYVHKLDNLAWCEVD
                     DEDHWARATTIVYPIIKAREDITHTVKRNILLNPGPATTTDTVKYAQIVEDICPREKEF
                     GETMEFISAELTKFVGNPEKYTTVLFGGSGTAAVESILSSVIDNGTVVIINNGPYGERM
                     CKIAEIYGLNYLEYKSSAEQAIDMNDLEIFIKKISTNVSYLALVHCETSTGLLNDIEQI
                     GKFCAVKNIEMIVDAMSSYAAVPIDMQKMNISYLAASANKNLQGMAGVSFVIANKARLE
                     KTEQIKPRNLYLHLYDQYRYFQMNKQMRFTPPVQTMYALKQAIIETQWEGIEKRYERYS
                     KSWTTLTDGITRLGLTYLVPEIHHSKIITSIIEPSHKKYNFDIMHDFFYKQGFTIYPGK
                     IDKLETFRIANIGDITYREIERFLHLLEQYLKGLKGE"
     aSDomain        complement(8185..8991)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_HW369_RS23990_Aminotran_5.1"
                     /evalue="2.30E-29"
                     /label="HW369_RS23990_Aminotran_5.1"
                     /locus_tag="HW369_RS23990"
                     /protein_end="535"
                     /protein_start="266"
                     /score="94.4"
                     /tool="antismash"
                     /translation="YAQIVEDICPREKEFGETMEFISAELTKFVGNPEKYTTVLFGGSG
                     TAAVESILSSVIDNGTVVIINNGPYGERMCKIAEIYGLNYLEYKSSAEQAIDMNDLEIF
                     IKKISTNVSYLALVHCETSTGLLNDIEQIGKFCAVKNIEMIVDAMSSYAAVPIDMQKMN
                     ISYLAASANKNLQGMAGVSFVIANKARLEKTEQIKPRNLYLHLYDQYRYFQMNKQMRFT
                     PPVQTMYALKQAIIETQWEGIEKRYERYSKSWTTLTDGITRLGLTYL"
     gene            complement(9782..10912)
                     /gene="aepY"
                     /locus_tag="HW369_RS23995"
     CDS             complement(9782..10912)
                     /EC_number="4.1.1.82"
                     /GO_function="GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="aepY"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081821.1"
                     /locus_tag="HW369_RS23995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="WP_195695310.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 6e-09, bitscore:
                     34.1, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 8.2e-14, bitscore:
                     50.0, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISTSVFGTQLKKLGFQFYSGVPCSYLKHLINYAINECEYVAAAN
                     EGEAVAIAAGASIAGNKAVVLMQNSGLANAVSPLTSLIHPFKIPVLGFVSFRGEPGCPD
                     EPQHELMGEITPALLNTMKIKWEYLSFDFAEVKKQLQRANQAVENNESYFFVVKKGTFE
                     PVKLLEKSIKNVTPKMKSEKITTDELPLRYEALQVVNSLKENDTIQLAATGKTGRELYE
                     IEDARNNFYMVGSMGCVSSIGLGLALSRNEKNIITIDGDGALLMRMGNLATNGYYCPEN
                     LLHILLDNQTHDSTGGQHTVSHNINFVDIASSCGYQNSIHVHNLNELETYIKQWKNDKK
                     LTFLYLKIAKGSKKNLGRPEMKPYEVRERLQVFLDD"
     gene            complement(10905..12422)
                     /gene="aepX"
                     /locus_tag="HW369_RS24000"
     CDS             complement(10905..12422)
                     /EC_number="5.4.2.9"
                     /GO_function="GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="aepX"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) phosphonates-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055492.1"
                     /locus_tag="HW369_RS24000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="WP_195695311.1"
                     /sec_met_domain="phosphonates-like (E-value: 2e-137,
                     bitscore: 455.0, seeds: 13, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 5e-126, bitscore:
                     418.0, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 7.7e-63, bitscore:
                     210.5, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKTKQLRELIESKQLEFIMEAHSALSAKIVEEAGFKGIWASGLS
                     LSAALGVRDNNEASWTQVLDILEFMSDATSVPILLDGDTGYGNFNNARRLVKKLEQRDI
                     PGVCIEDKLFPKTNSFIKGEAQPLADINEFCGKIKAMKDSQNDEDFVVVSRVEAFIAGW
                     GLKEALKRAEAYRQAGTDAVLIHSKRSDMTEIEAFMKEWGNRLPVIIVPTKYYSVPTDK
                     FSDLGISLSIWANHNLRSSITAMQKTSAQIYYDKSLINVEKNVSPLEEVFRLQKAEELD
                     MAEKLYLSSANHSTNAMILVKESANEALITEQINQLKKVGVTNSIKVDSNKAMEDKAFP
                     NKGELYHLYAARDKLGTATLISDSNIVYKTHVFEELIHESGDITIVAGADYELKGNQTS
                     YVTSKLPYSKQLYSKTVDLIQMSCNPNSNNIHGEFIGLWKVSGKGTEVVKKALEELAQR
                     KDFAQLTFSDFFNYINLIHPVAVKYTVGSWIDIETYTKIQKDGGKND"
     gene            complement(12436..13845)
                     /gene="murF"
                     /locus_tag="HW369_RS24005"
     CDS             complement(12436..13845)
                     /EC_number="6.3.2.10"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="murF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013055491.1"
                     /locus_tag="HW369_RS24005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="WP_195695312.1"
                     /transl_table=11
                     /translation="MKHFTLNEILKQTGGDLCYGSGNPVIKHAINYAKKDIEDHTLIFH
                     LDREPIRGKYWKENHSIVVITDHPELCTNLEGNIILVKTDQLEEAYWKFIEYYRGLFDL
                     PVIGVTGTCGKTTTKEMIRQILIEDYKVKATWMSMNSMSVNLRYLTGIDDTTEVAVFEM
                     PVAYPGYLKVACRYFQPQIRILLNIGVHHLADCETPEEYMKAKAEIVEGLDPNSGILIL
                     NADDQNIKKVLDVSHLQNVVYIGKGEDSQFQAKDIKYANGGMAFTLKHQNKEYDAFIPG
                     YGEHNVYNALAAIAAVFYVGVDISVATQRLSLFEQVEEHLEFKRGMNECTVINDTWNSS
                     PLSMATALKVLGDMSKEKKSIALLGYMPQLGEGSYAIKQYEDMGKKAAESKIDILVVVG
                     EEAKAIGTGALKCGMDPNKVYFCNTGDEVYNVLAPYLNGNTIILLKVTHRVMKKPTFKE
                     LRNKLIPDNEE"
     gene            complement(13842..16463)
                     /locus_tag="HW369_RS24010"
     CDS             complement(13842..16463)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081818.1"
                     /locus_tag="HW369_RS24010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YheC/YheD family protein"
                     /protein_id="WP_195695313.1"
                     /transl_table=11
                     /translation="MDPMKEELGILSDKEMTLQTLNLNNIPSVELVDPKTCSYPVIGRK
                     YGHHSGRDIVIVNTKDQAIYEGYDYFTKIYAIDKEYCLEVEGLSVKTVQVVTSEHVVFN
                     EIPIRTQAFGWKLEQINGMDVPEMLMNVAIRALYVTGAKSGFVKMGVLENGECIVTDIN
                     SSENEWIENPLKPSVPFSMGADVEFMLSCDGELLPASTFFSLEGAVGCDERQIEQDSGE
                     YPLVEVRPEKANSSTELFENIQKLIEKASAQVPYENIHFRAGSMPFSGYQCGGHIHFGI
                     PLSLSLLRALDHYLAIPVALIEESKTAKLRRKTNHGGLGRYREKPYGFEYLTLSSWIID
                     PRITLSTLALAQLVATHHHELKSEFLFHPLTQRAYYQGNKTFLKRMWKDIKANLMKTSS
                     YPHYQNELSFLFEMIEKEIPCDESSDIRRNWNVKISKEIYDRGHIIQIPKKLRLKYGLK
                     EGQSTIISAGKAISTATVHSYPFSFRHPNMVQLSKSLRDKLSLPKDWCPKLSASEGIIT
                     LGPIIGILANRPFERQTTYFHHLCRLATEKRMLVYVFEPEDIDWEKKLVKGTTINGEGL
                     FPFPAVIYDRYFIDGRKNILIDEVRAKLQAIYKIPFVNSSNLFQLTGDKWATYELLMKE
                     YEEFLPESRLVQNSADIAEMLDSYGEVYLKPLGGALSKGVMRIVRRPTGIFWFDLNKKE
                     LHQFSNMEELFTLLSPLMKNNPYLVQEGIRRKQHKDKNLEIRVYMQKNEKQIWLRTGMV
                     ARLTGEDVLTEDSETNMRLSKILNSLYPDPTDRRLIINQLAKISKNIVATVEEKVGPFG
                     ELAVDLCIDQYGSIKLLEINAKPDSLFSQIRAYKLRTLAGIRLLNYASSLAGYEEEKED
                     LT"
     gene            16709..17383
                     /locus_tag="HW369_RS24015"
     CDS             16709..17383
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013081817.1"
                     /locus_tag="HW369_RS24015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_195695314.1"
                     /transl_table=11
                     /translation="MALKFIHGNYLKYNKGTGFISCHVDALHAEPPAADLFTDKIVENV
                     VKKTGCAGIISTVSRTVADLNRNLNKTNYEGIQEYRSTIQEIIKSLKIIDSNNFLLTPY
                     LHLSFHGMKDTHYGPHAIEVGTLYGQSCSKEVKDWFQQALSKKFENSFPKIHVIFDNKF
                     IGNESIAFHRQGDNRSYTGYSHNFNTFQIELSRTLRSKHRSAITNIFSEIIHDFQQTFV
                     TT"
ORIGIN
        1 ttcttatata tcctataact tgaaatctat ataaaaaaga ggttgggata taactaaatg
       61 aatccaatct aaagacgaaa aaatgggata catgagctca tatggctcgc ttgttccatc
      121 acggttgatt tccgtgcaag acttcgcttt ccgcgtgcgt ccgctgagcc tcctcgtcgc
      181 taaacttctg ccggtctcac ctattccgct tttcccgcag gagtcttcgt cttgtcctcc
      241 aatcaaccgc tagaagcaac tacatacata aaatctacgt tcgtcctatc aataaaaaaa
      301 accgaacgag ttagattctc cattaagaat ctaactcgtt cggatcttca actaaagtac
      361 ttttagctac agatgagccg gaaaatgcgg aagcacttct tcgccaagct catgtataat
      421 ttcttcagcg ggacgcttac tatcaaaaag agcaaaaaag agatgattaa ctcctatttc
      481 tttataaata gaaagaagct caattaatgc attacgacct gctcgaaatc ctaaacgaat
      541 tggcgttggc atttcatttg gatcttctgc caaatctaga tgcataggtt gcgtaaaagg
      601 tttaaaaacg cctggatgat agatgtcaac cagtttcctc cattgtgtaa tagcttctgc
      661 ttgaaaatga ggatgacgag gataatacat ccagccgtct ccatactggg caaaccactc
      721 catattttgc tgactgtatc ccgtgatcat cgttggaata cgtttcgttg gcttaggaac
      781 cacatttgct ccttgaacag aacccaaagt agactggata gatggaaaat cttgatttaa
      841 cgctgcttct aaataatgaa aagcctctgt aaatcgcttt cctctttctt catgaggaat
      901 acctagtcct cgaaaatctg cttgacggtc tccagaagat acgcccataa tcaaccgctc
      961 aggaaaaagc tgatcaatgg ttgccatttc tttagctacg cgaagcggat gacgcaaagg
     1021 cagcacaata gcagatgtac taagagcaat ttcttttgtt tgactggcaa ggtaagtcaa
     1081 ataaataagc atatcataaa tttgacccgt tgctggatcg ccaaatgatg gatcttgaag
     1141 aaggacatca cggaaccata agcttgtaaa tccatattgt tctgaaagct gcgtgagctc
     1201 tacttgtttt tccatcgtag gcgtggcata ctgataattt tcaagcggca tatgcaaacc
     1261 taacgttaac tcattctcct tgtacatacg acgatacgcc ttatgatgtt gaaatgcagt
     1321 catttgcttc ctctcctttt atagataaga gatcatcgca aaaggcgatg atctcttgtt
     1381 ttattttgct tttgcttcta ttgagcggct ccatgctgtt aataagaccg ctcctaaaac
     1441 cataactcct ccaatccaag gagtatgaac aagaccaatc gaatcaacaa tcaggccccc
     1501 tacaaaggag ccaatagcaa ttccaacgtt aaatgcagca atattaagcg cagacgctac
     1561 attcactgct gaaggaacat acttttcagc taagttcacg accagcactt gcagtccagg
     1621 tacgttcata aatgccaata agcccatggc aaaaatcgta atcgttcccg ctactttaaa
     1681 tgatgcagta aacgataaaa taactaagat aacggcttga attagaaaca tccaaaataa
     1741 tgcttttaac gggtctttat tcgcagcttt tcctccgatt gtatttccga tggctaccgc
     1801 tacaccgtat accagcagta agatactaac cgcgcttggt gcatatcctg ttagtttttc
     1861 aagaataggt gctaaatacg tataggctac aaacgttccg ccatatccta aggcagtaat
     1921 agcaaacgat agtaaaagcg gcccgttttt caaaattttc aattgatcac taaactttgc
     1981 cggcggcgct tcttttaagt ttttaggcac taaaagagca cttgcaataa tggcaataac
     2041 cccaagtaac gctactcccc aaaatgttgc tctccagccg aaagcttgtc cgataaatgt
     2101 accaagcgga acacctgtaa ccgttgcaac tgttaaacct gtaaacatta aagcaatggc
     2161 acttgcccgc ttatcttccg gtaccaaatc agcagcgata gtcgcaccga tagaaaagaa
     2221 aattccgtgt gaaaatgcag taataattcg tgctataagc agcagtccaa agcttgttga
     2281 taaagctgct actgagtttc ctacgataaa aataaccatc aatagcatta acagtgattt
     2341 tctgctcatt ttacttgtta aagctgttaa tacgggagct ccaaatgcca ctcccattgc
     2401 gtatcctgaa attaataacc ctgctagtgt aatggaaata tttaaatcat ctgcaagcga
     2461 tgataataaa cctactggaa cgaactctgt agttccgatc ccaaaggcgc taatagctag
     2521 cgctaatagc ggcaaagcac cgcttttttt actttgttgt gtttgtgaaa gtgtattcac
     2581 tttttaacgc ctcctgttcg ttataataac ttctttataa tagcttcttt tatggctgat
     2641 tagcttaggt acctttcata tattcgctaa ctcgccatca acaattgtta ttatgagcga
     2701 tgtaaaacca aagaaaaagt acgcactttc aagtgatata ggtacctgga ggttacaata
     2761 agcgtaaata ctaaagtcat gaactattgg gtattctaaa aaaatgctta cacctagaaa
     2821 attctgctct atagtggtat gataaacatc agaaaaaatg cgaaaccatg catttaaccg
     2881 ttatctctag aacaaggaga gttgttaacg tgaaaaaata taatattcca gtggaagcgg
     2941 cactagaagt cattggaggc aagtggaaag tcgttattct ttgccactta attaaaggga
     3001 caaagcgccc tggagaatta aaaagaatta tgccgcgcgt tacacaaaaa gtattaacgc
     3061 agcagcttcg tgaacttgaa gcagacggca tcattgttag aaaaatgtac aatgaaatgc
     3121 cgcctcgagt agaatattct ttaacaaatt acggatggtc tttaaaaagc attcttgatt
     3181 cattatgctc ttggggagaa catcatattg aaaaaacgta tcctgataaa gaagctgtac
     3241 ttatgcagcc tgtagaagaa gtcactgttt ctaattaaaa aagctgacta gatagtcagc
     3301 tttttcattg ccttactttc tttgtttttc agacgatgta cgtattcagg aggagcccat
     3361 atgacagaac aatcaaaaaa gaaaattagt cttcaagatg ctattaaaca gcagcttgct
     3421 aataagaaaa accaatcttc aaataaagct aagaacagca gtactaatca agcaaccaaa
     3481 aagctgacaa gtcaacaaac aaaaaaaacg aataatcagc gaaagagaac aggtgtataa
     3541 tgaacggaca aaatagaaaa gacgtagctc ctggaattaa agtcgacatt gttttaaaag
     3601 cagatcagcg ctctggaaaa ctgacacatg gaacggtcaa agacattctg accaattcaa
     3661 gctttcaccc tcacggcatt aaagttcgtt taacagatgg acaagtcgga cgtataaaag
     3721 aaatttaccc aaattaaaaa agcatgtatt ctacatgctt ttttaattta tccttcaaat
     3781 aaatctactt atcatttcct acttttgtta aaataaagaa gcactgtcgt cccctttctt
     3841 tcccttggga attcacgcaa ggaaaaaaag gtggtggata gtctgagaca aatcctcaag
     3901 tcatttttcc tagatgctga gaatgttcag ctcctaacgg cttacgtcga gcttttagga
     3961 gccatagtcg ctcttatttt gctatttttc cagtagcaaa ataagtaatc accgataagt
     4021 atcgtctaaa aggttcttca tccataaggc tcgtgtgctg attgtcttta gggaatggtg
     4081 tttggcggca cttccctata gtaaaaagac agcttccttg cagaaatgga caaggtgtaa
     4141 tcaactacgg ccgattatgg gtgcgggacc tttttcttta ttagtctatg tctttttttc
     4201 ccttcccata cacaaaaata aaaaaacctc cgagttggcg gtctcgaagg ttttttctag
     4261 tgaatcgttt tcgacaaatc ctcatccggg ctaacaaggt tcagatctaa taagattatg
     4321 tgatgaattt cttcacctta caagctaaac ttgtcaaagc taagaattca ttcgcttgtt
     4381 aagaataact gaacgtgcgc tccagattgt catttttcct agcctcagta tatcatactt
     4441 gttgaaaaca ggtaaaaaaa gtgttcgaca aagcttgtca gcagctcttc acctatgtca
     4501 tatttttcaa aacaaaaaac attttacaaa tatgaaggaa tttcatacta ttagataaaa
     4561 gataaaacat agaagataga agtattctga cttggagagg atttcaatga agcaacctca
     4621 gttagaaaaa gaaattcgag ccttacaatc agatatttac caattagcta agaaaacaag
     4681 cagctattct cagggtgaga ttttaaaatt aagtcaaaag ctagatcaaa aaattgttag
     4741 ttaccaaaag ctttttaacc atactaaata agagccgcgt aggactttca cttgctatcc
     4801 ctcaggtacc ataaacctac aaataaaaca gatatgtttt attcgcaata ggtacatctc
     4861 tgcttcccta attaataggg aagtttttta tttagaaaat taaaaccttc tcctttcctt
     4921 tgattagttg tcatacaaat tgggtattca atcagtaggc cttaaaacct atataagaaa
     4981 tataaaggag agaatgcatg atgagtaatg ctgatactac cgaaacaaaa aaaggagctg
     5041 acttactagt agaaagtttg gcaagtcaga atgtgcctta tatttttggc attccaggag
     5101 ccaaaattga tggcgtattt gatgttttga aagaaaaagg gccggaagta attgtttgcc
     5161 gccatgaaca aaatgctgcg tttatggcag ctgctatagg aagactgaca ggtacgccgg
     5221 gcgtttgcct tgtcacatca ggtccaggtg ccgctaattt agccactggc cttgtcactg
     5281 ctacttctga aggtgatcca gtggtagccc tagcaggagc cgttccaaga gcagatcgat
     5341 taaaaaagac gcatcaatca atggataatg aaggattatt taaatctatc gccaaattca
     5401 gcgttgaggt agaagacgct gcaaacgttc cagaagtgat aacagacgcc tttcgcgaag
     5461 ctgtttcttc tccttcagga gcatcttttg tcagcttgcc gcaggatgta atggatagcg
     5521 aaacatcagc ttccactcta tccattttaa aagctcctac gattgggact gctaatttag
     5581 acgctgttgc ccaagctata cacactattc gcacagcaaa actacctgtc ttgcttttag
     5641 gtatgagagc aagtaaatct aaagcagtgg aatcaatccg tcagcttata gcagaacatg
     5701 accttcctgt ggtggaaacg ttccaaggtg cgggagctgt ttcacgagag cttgaaactc
     5761 acttttttgg gcgagtaggt ctttttcgca atcagcctgg agacgtctta ttgaagcatg
     5821 ctgacgtcgt cttaacaatc ggatatgacc cagttgaata ccctccttct ctttggaact
     5881 cagaaagtca aagaagcatt attcatttag atgatttaca agcacaagct gaccaccatt
     5941 atcagccgga aattgagtta gtaggggata ttccaagcac aattgacata ttagcggata
     6001 aactcccaca tttaatacgt tcaaaagaaa gtgcttcttt tttaaaaaca ctaaaaagca
     6061 aacttgaaca acctgacgat cagtacgagt tttctagtga cactcacctt catcccctac
     6121 aattaattga agctctgcag aactttgttg aagatgatac taccattaca tgtgacgttg
     6181 gttcgcatta catatggatg gctcgttatt ttcgttccta tgaacctcag catttactat
     6241 tcagcaacgg tatgcagaca ctaggcattg ctttgccttg ggccatttca gctgcactcg
     6301 ttcgccccca taaaaaagtt ctgtccatgt ctggagacgg cgggttttta ttttcagcta
     6361 tggagctcga gacagccgtt cggttaaact caccaattgt tcacattatc tggaacgatc
     6421 aaacctacga tatggttggc tttcaacagc agattaaata tggccgaaaa tctgcagtag
     6481 agtttagcag tgtagacttt gttacgcttg ctgaaagttt tggtgccaaa ggatttagag
     6541 ttaactcacc cgaagaattc ccggacactc tagcaagcgc ttttcgtgaa cgaggccccg
     6601 ttatcattga tgttcctgta gattataaag ataatgaaaa attaaataca cagctactgc
     6661 cagatcaaat taactgaaag gactgagtaa agaatgagca ataaagaaaa attaacgatt
     6721 caccaaactt ccactatgat cgctttatta gaaggagtat ttgacggtgt tgtcacatac
     6781 aaggatttag ctaagaaagg tgatttcggc atcggtacgt ttaatcaatt ggatggtgaa
     6841 atgattgcct ttgacggaga attttatcat attgctgatg gaaaagccgc acctattagt
     6901 aaagatgcaa aaacaccttt tgccattatg acaacctttt atgaagatat ctcttatcgt
     6961 gtagaaaagg aaatgaatcg tgaacaacta gaaatcttaa tgaacgagct tattcctagc
     7021 ccgaatttat tctatgcagt tcgcattgac gggaagttta aagaagtaaa aacgcgcacg
     7081 gttgcaaagc aagaaaagcc ctatccttct atggtagatg cggctgctga tcagcctaca
     7141 tttacatctt cagatgtaaa tggaacacta gcaggttttt acacgccaga atattcttca
     7201 ggcatcggtg ttcccggcta ccacctgcac tttattgatg aatataaaaa agaaggggga
     7261 catgttttag atgtctccgt tcaggatgtt acaattcaaa tctgcaaaaa aacgaaatta
     7321 aatctcaatt tatcggaaac aagtgaattt ttaaatgcta acttagaaga ccatgatgta
     7381 gaagaagaaa ttgcgactgc tgaataaaca acgatacttt tactgataaa aaacgctcat
     7441 cgtcatgcat gacgatgagc gttttttatt taatcgagtt caggcttcat ttctagcaca
     7501 acacagaaac cttccgccta tacataaaaa gagcccgtgt cttttaaaac atagaaaaat
     7561 ggtatttcag cctcttttag acatctagcc attcgatcct taaagttatc taatttaatt
     7621 atattttttg cttcttctaa cgtaaaatat cccacatctt gcgtttcact ggagacagaa
     7681 atttccccgc taatcgcttt tccctttaat aaaataacag tagtagatcg tttaacatct
     7741 tgttctactc ctagaaagtt ggtcacttca ataacaattc cagattcttc tttaacttct
     7801 cgtatacccg catcttttat cgattcacct gcttcgacaa aaccaccggg aaattcccat
     7861 ccccgtctat ggttcttgac tagaagcacg tggtgatctt gattgattac cataactgct
     7921 acagctaatt tttgattcaa ctttttctca ctctcctttt agacctttaa gatattgttc
     7981 aagaaggtga agaaatcttt ctatctctct atatgtgata tcaccaatat ttgctattcg
     8041 aaacgtttct aatttatcaa ttttaccagg ataaatggtg aatccttgtt tataaaaaaa
     8101 atcatgcata atatcaaaat tatacttttt atgggaaggc tcgatgatag aggtaataat
     8161 tttagaatga tgtatttcag gaaccaaata ggttaaccca agccgagtaa ttccatccgt
     8221 taaagtagtc catgattttg agtagcgttc atatcgtttc tcaattcctt cccactgagt
     8281 ttctataatt gcctgcttta aagcgtacat agtctgaaca ggaggagtaa aacgcatttg
     8341 tttgttcatt tggaaatatc gatactgatc atataaatga agataaaggt tacgaggctt
     8401 tatttgctcc gttttttcta accgggcttt atttgctata acaaacgaca cgcctgccat
     8461 tccttgaagg ttcttatttg cacttgcggc cagatagctt atattcattt tttgcatgtc
     8521 aattggaaca gctgcatacg aactcattgc atccacaatc atttcgatat ttttcactgc
     8581 acaaaatttc ccaatttgtt caatatcatt aagaagccct gtggaagttt cacagtgaac
     8641 aagcgccaga taagaaacgt tcgttgaaat cttttttata aaaatttcta agtcgttcat
     8701 atcaatagcc tgctcggcgg agcttttata ttctaaataa ttcagtccat aaatttcagc
     8761 gattttacac attctttcac cgtatggacc attattgata ataacaaccg taccgttatc
     8821 aattactgaa cttaaaatcg attccactgc cgccgttcca gatcccccga ataataccgt
     8881 agtatacttc tctggattcc ccacaaattt tgttagttca gcagagataa attccatcgt
     8941 ctctccaaat tctttttctc tcgggcaaat gtcttccact atttgagcat atttgacggt
     9001 atctgttgta gttgccggcc cggggtttaa gagtatattt ctcttaactg tatgcgtaat
     9061 atcttctcga gctttaataa tcggataaac aatagtagtt gctcttgccc agtgatcttc
     9121 atcatccact tcacaccatg ccaaattatc cagtttatga acatacatat ttgctttttc
     9181 ggaaatttta acgaggccat attcataatc gatcgtttga ttaaactgaa acagcgtatt
     9241 tgcttcatca caaagtctct gaaatgtagc ataagagagt ttggttaaac caactagttc
     9301 tgcatagata ctattcaact cttctttttg ttttgacata ttcttaaggt tattattttc
     9361 atcagcttca atataaactt catcgccagc accgttcaac ttactcgcta gtatgacatc
     9421 tgatctcgaa tggtttacta atgtttccaa cgcattcttt tcataaatta catctgattc
     9481 caatagcaaa aaatcttctt taatataatt tttaaattga tatagcgtaa acagacttcc
     9541 tgttgtttcg tatctagaat tataaacaca ttttatttga ggatatctaa ccattagctt
     9601 ttcatattct tctttcatat atcctgttcc aataaaaatc gtcttgactc ctgcttctat
     9661 taacctcgaa atggaatgtt caataatcga tttttcatca atcatcaaaa aacctttagg
     9721 acggtttcca gctcttttac gaatcctgct ccccatccct gcagctagaa taacagctgt
     9781 tttaatcatc taaaaacacc tgcagtcttt ctctgacttc ataaggcttc atttcaggac
     9841 gccccaggtt ctttttagat cctttcgcta ttttaagata taaaaacgtt aattttttat
     9901 catttttcca ctgttttata tacgtctcta actcgtttaa gttatgaaca tgaatggaat
     9961 tttgataccc acaggaagaa gcaatatcta caaaattaat gttatgagag accgtatgtt
    10021 gtccccctgt agaatcatga gtttgattgt ctaaaagaat gtgtaataaa ttttccggac
    10081 aatagtaccc gttggttgct agatttccca tgcgcataag caaagctccg tctccatcaa
    10141 tcgtaatgat gttcttttca ttcctgctca aagctaagcc taaaccaatg gaactaacgc
    10201 accccataga tccaaccata taaaaattat ttcttgcgtc ttctatttcg taaagctctc
    10261 ttcctgtttt tcctgtagcg gccaattgaa tagtatcgtt ttcttttaat gaattaacta
    10321 cttgtaacgc ctcatacctt aaaggtaact cgtcggttgt tatcttttca cttttcattt
    10381 taggagtaac gttttttatt gatttttcta aaagcttaac tggctcaaac gttccttttt
    10441 ttacaacaaa gaagtagctt tcattattct caaccgcttg attagcccgc tgtaactgtt
    10501 ttttaacttc ggcaaaatca aaagataaat attcccattt tattttcatc gtatttagca
    10561 gggcaggagt aatttctccc attaattcat gctgcggttc atctggacat ccaggttctc
    10621 cgcgaaaact gacaaatcca aggacaggaa ttttaaacgg atgaataaga gaggttaaag
    10681 gggaaacagc atttgctaat ccggagttct gcattaaaac aaccgctttg ttgccagcaa
    10741 ttgatgcccc agcagcaatc gctaccgctt ctccttcgtt tgctgctgca acatattcac
    10801 attcgttgat agcgtagtta ataagatgtt taagatagga acaaggaacc ccgctataaa
    10861 attgaaaacc tagttttttt aattgagttc caaatacgga tgtactaatc atttttccca
    10921 ccatcttttt gaatctttgt atacgtttcg atatctatcc atgaccccac cgtatattta
    10981 acagcaaccg ggtgaattag attaatataa ttaaagaaat cagaaaacgt gagctgagca
    11041 aaatcttttc tttgtgccag ttcttccaaa gcttttttta caacctcagt gccttttccg
    11101 gaaactttcc aaagaccaat gaattcaccg tgaatgttgt tagagtttgg attgcacgac
    11161 atttgaatca agtctactgt tttggaatac agctgcttgg aatagggaag tttagacgtt
    11221 acataagatg tttgattccc ttttaactca taatcagctc ctgctacgat cgtgatatcg
    11281 ccactttcat gaattaactc ctcaaaaaca tgtgttttat atacgatgtt actatcgctg
    11341 attaaagtag ccgttcctag cttatctctt gcagcataga gatgatataa ttctccttta
    11401 ttaggaaatg ccttatcttc catagctttg ttggaatcta cttttatact atttgtcacg
    11461 ccgacttttt ttaactgatt tatctgctca gttattaaag cttcatttgc tgattctttt
    11521 accagaatca tagcgttcgt gctatgattg gcagagctta aataaagttt ctctgccata
    11581 tcgagctcct cagctttttg aagacggaaa acttcctcaa gcggagagac atttttttct
    11641 acgttaatca aacttttatc gtaataaatc tgagcggacg ttttttgcat agctgtaatg
    11701 gaagatctta agttatggtt ggcccaaatg ctcaagctaa ttcctaaatc actaaattta
    11761 tcagttggaa ctgaataata tttggttgga acaataataa ccgggagcct gtttccccac
    11821 tctttcataa aagcttcaat ctctgtcata tctgaccttt tactatgaat caaaacggcg
    11881 tcggttcccg cttgacgata agcttctgca cgttttaaag cttccttcag cccccaccct
    11941 gcaataaaag cttctacacg cgataccact acaaaatctt cgtcattttg gctatctttc
    12001 attgctttaa ttttgccgca aaattcgtta atatctgcaa gcggctgtgc ttcaccttta
    12061 ataaatgagt ttgtttttgg aaacaattta tcttcaatac atacgcctgg aatgtctcgc
    12121 tgctccaatt tcttaactaa tcttcgtgca ttattaaaat ttccatagcc tgtatcacca
    12181 tcaagaagaa ttggaacgga cgttgcgtca ctcataaact ctaagatgtc taatacctgt
    12241 gtccatgaag cctcgttatt atctcttaca cccagtgctg cagataatga caggccgctg
    12301 gcccatattc ctttgaaccc tgcttcttcc acaatctttg cagatagcgc actgtgagcc
    12361 tccataataa actctagctg tttactttct atcaactctc ttagctgttt agtcttcttc
    12421 atgtctatcc tccttttact cttcattgtc tggaataagt ttatttctta attctttaaa
    12481 agtaggtttt ttcataacgc gatgcgttac ttttaataaa attattgtat ttccatttaa
    12541 atatggagct agcacattgt atacttcatc accagtgtta caaaaataga ctttgttagg
    12601 atccattcca cattttaaag ctcctgtccc aatagctttc gcttcttctc ctactaccac
    12661 taaaatatca atctttgatt ccgctgcttt ttttcccata tcttcatatt gtttaattgc
    12721 ataagatcct tctcctagct gaggcatgta acctaataaa gcgatagact ttttctcttt
    12781 tgacatatca ccaagaactt taagagctgt agccatcgat aaaggtgaag agttccatgt
    12841 atcattaatt accgtacatt cattcattcc tcttttaaat tccaaatgtt cttctacttg
    12901 ttcaaataag ctaagtcttt gtgtggcaac ggaaatatca acgcccacat aaaatacagc
    12961 agcaatggca gccagtgcat tatacacatt atgttcacca tatccaggga taaaagcatc
    13021 gtattctttg ttttggtgct ttaaagtaaa agccattcct ccattagcat atttaatatc
    13081 ttttgcttga aattgggaat cctcaccttt acctatatat acaacatttt gaagatgact
    13141 aacatcaagc actttcttaa tattttggtc atctgcgttt aaaatcaaaa tgccgctatt
    13201 tggatctaag ccttctacaa tttcggcttt tgctttcata tattcttcag gtgtttcaca
    13261 atcagcgaga tggtgcacgc ctatatttaa tagaattcgt atttgcggct gaaagtagcg
    13321 acacgcaact tttaaatagc ccggataagc gaccggcatt tcaaaaacag ctacttctgt
    13381 cgtatcatca atacctgtta aataacgtag atttacagac atagaattca tactcatcca
    13441 cgtagctttc actttataat cttcaattaa tatttgccgg atcatctctt tcgtggtcgt
    13501 ttttccacaa gttcctgtaa caccaataac aggaagatca aaaagccctc ggtagtattc
    13561 gataaatttc caataagcct cttctaattg atctgtttta acaagaataa tatttccctc
    13621 taagttcgtg cacagctcag ggtgatctgt tataacgaca attgaatgat tttctttcca
    13681 atacttccct ctaatcggtt ctctatctaa atgaaaaatg agtgtatgat cctctatatc
    13741 ctttttagcg tagtttatag catgtttgat aacaggattt cctgatccat aacaaaggtc
    13801 tccccccgtt tgtttaagaa tttcatttaa tgtgaagtgc ttcatgttaa atcctccttt
    13861 tcttcttcgt aaccagccag tgaagacgca taattgagca gacgaattcc cgccaaagta
    13921 cgcagcttgt aggctctaat ttgtgaaaat agactatctg gttttgcatt tatttcaagt
    13981 aactttatgg aaccgtactg atctatacat aaatctaccg ccagctcccc aaaagggccc
    14041 actttctcct ctactgttgc aacaatattt ttagatattt tagctaattg gtttataatg
    14101 agccttctgt ctgtcggatc gggatataaa ctgtttaata ttttactgag gcgcatattc
    14161 gtttcagaat cttccgttag cacatcttca ccggtgagac gcgcaaccat acccgttcga
    14221 agccaaattt gcttttcatt tttttgcata taaacccgta tttctaaatt tttatcttta
    14281 tgctgttttc tgcgaattcc ttcttgaaca agataaggat tgtttttcat caaaggagag
    14341 agcaaagtaa ataattcttc catattactg aattgatgca gctctttttt attaagatca
    14401 aaccaaaaga tgcctgttgg tcggcgaacg atacgcatga ctcctttact taaggccccg
    14461 ccaagcggtt ttaaataaac ttctccgtag ctatcaagca tttctgcaat gtcagcagaa
    14521 ttttgtacga gacgtgactc tggaagaaat tcttcgtatt ctttcataag aagttcatat
    14581 gttgcccact tatctccggt aagctgaaac aaatttgaag aattaacaaa aggaatttta
    14641 taaatcgcct gaagttttgc ccttacttca tctattaaaa tattttttct accatcgatg
    14701 aaataacggt cataaataac agcaggaaaa ggaaaaagcc cttcaccgtt aatggttgtt
    14761 cctttaacca attttttctc ccaatcaata tcttcgggtt caaacacata tacaagcatt
    14821 cgcttttcag ttgctaagcg acatagatga tggaaatagg ttgtttgtct ttcaaaagga
    14881 cggttagcaa gaatgccaat gataggacct aaagttatga tgccttctga agctgatagc
    14941 ttggggcacc aatctttcgg gagagatagt ttatcacgga gagatttaga gagttgaacc
    15001 atatttgggt ggcgaaatga aaaaggataa gaatgaacag ttgcagttga tattgccttt
    15061 cctgcactaa taatcgtaga ctgcccctct ttgaggccgt attttaaacg gagctttttg
    15121 gggatttgga taatatgtcc gcggtcatag atttctttag aaatcttcac attccaattc
    15181 ctgcgaatat cacttgattc atcacaagga atttcttttt ctatcatttc aaatagaaaa
    15241 gatagttcat tttgataatg tgggtagctg gatgttttca taagattggc cttgatatct
    15301 ttccacattc gtttaagaaa ggttttattc ccttgatagt aggcacgctg cgtaagtgga
    15361 tgaaataaaa attcgctttt taattcatga tgatgggtag caactagctg agcaagagct
    15421 aaagtggaga gagtaattct agggtcgata atccaagagc ttaaagttaa atactcaaat
    15481 ccatacggtt tctcgcgata gcgtccaaga cctccatggt tcgttttccg tctgagttta
    15541 gcggtcttag attcttctat cagcgctact ggaatagcaa ggtagtggtc aagagctctt
    15601 aataaggata aagaaagggg aatgccaaaa tgaatatgtc ctccacattg ataccctgaa
    15661 aacggcatgc tgcctgcccg aaaatgaata ttttcatatg gtacctgtgc agatgctttt
    15721 tctattaatt tttgaatatt ctcaaatagt tctgttgatg aattcgcttt ttcaggccta
    15781 acttctacta atggatactc accgctatct tgttctattt gcctttcatc acagcctacc
    15841 gcaccttcaa gagaaaaaaa agtagaagct ggtagaagtt ccccgtcaca gcttaacata
    15901 aactcaacgt cagcacccat gctaaaagga acactaggct ttaatggatt ttctatccat
    15961 tcattttctg atgagttaat atctgtgacg atacactctc cattttctag tacccccatt
    16021 ttgacaaagc cgcttttagc tcccgttacg tagagcgctc gaatggctac gttcatcagc
    16081 atttctggta catccatgcc gtttatctgt tctaatttcc agccgaaggc ttgcgttcga
    16141 atgggtattt cattaaatac aacatgctca ctggtcacta cttgtactgt ttttacgctc
    16201 agtccttcta cttctagaca atattctttg tctattgcat atattttcgt aaaataatca
    16261 tatccttcat atattgcttg gtcctttgta tttacgataa caatatctct tccgctatgg
    16321 tgaccatact tcctccctat cactggatat gaacaggttt tggggtcaac taattcaaca
    16381 gacggtatat tattcagatt aagagtttgc aacgtcattt ctttatcaga taatatccct
    16441 aactcttctt tcattggatc cattttaaag cctcctaatt agaactttct atcgtatttt
    16501 gctactttaa tgccctataa caataatcag ctactcacat agtatgtaac tatacaaaat
    16561 actcctgtac atttatctat ttatttccac catctctaaa aactttttat gtatttactt
    16621 aacacgtgta ataattcacc gattttattt ttactgcatt tattatagaa aggatagatt
    16681 tcaaacgata taaaaggaga aatagtcaat ggcattaaaa ttcattcatg gaaactactt
    16741 aaaatataac aaaggaactg gttttataag ctgtcacgta gacgcacttc atgcagaacc
    16801 acccgcggct gatttattta cggataaaat cgtagagaat gtagtaaaga aaacaggatg
    16861 tgctggaatt attagtacgg tttcccgaac cgtagcagat ttaaatagaa atctaaataa
    16921 aacgaactat gaggggatac aagaatatag aagcacaatc caagaaatta tcaaatcttt
    16981 gaagataata gatagcaata atttccttct aacaccctat ttgcatttat ctttccatgg
    17041 aatgaaggac acacattacg ggccccatgc aattgaagta ggaaccttgt atggacaatc
    17101 ttgctcaaag gaagttaagg attggtttca acaagccctg agtaaaaaat ttgaaaattc
    17161 gtttccaaaa attcatgtaa tatttgataa taaatttatt ggaaacgaat caattgcttt
    17221 tcacagacaa ggtgacaata ggagctatac aggctacagc cataatttta atacgtttca
    17281 aattgaatta tcccgaactc tgcgcagcaa acatagatct gctataacaa atatattttc
    17341 cgagattatt catgactttc aacaaacgtt cgtcactact tagtttttag gaatcttttc
    17401 cgtctactat ttcttttata ta
//
