LOCUS       NZ_JADMPO010000043     11375 bp    DNA     linear   CON 29-FEB-2024
DEFINITION  Mediterraneibacter gnavus strain D52t1_170925_A9
            NODE_43_length_11375_cov_21.6898, whole genome shotgun sequence.
ACCESSION   NZ_JADMPO010000043
VERSION     NZ_JADMPO010000043.1
KEYWORDS    WGS; RefSeq.
SOURCE      Mediterraneibacter gnavus
  ORGANISM  Mediterraneibacter gnavus
            Bacteria; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae;
            Mediterraneibacter.
REFERENCE   1  (bases 1 to 11375)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Quantification of discrete gut bacterial strains following fecal
            transplantation for recurrent Clostridioides difficile infection
            demonstrates long-term stable engraftment in non-relapsing
            recipients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11375)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, 1470 Madison Ave, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 27.07x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/29/2024 03:23:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,428
            Genes (coding)                    :: 3,362
            CDSs (with protein)               :: 3,362
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 6 (23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 15 of 66
            Pseudo Genes (incomplete)         :: 56 of 66
            Pseudo Genes (internal stop)      :: 5 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:46:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 11375
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADMPO010000043.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11375
                     /collection_date="2017-09-25"
                     /country="USA"
                     /db_xref="taxon:33038"
                     /host="Homo sapiens"
                     /isolation_source="stool"
                     /mol_type="genomic DNA"
                     /organism="Mediterraneibacter gnavus"
                     /strain="D52t1_170925_A9"
                     /submitter_seqid="NODE_43_length_11375_cov_21.6898"
     region          1..11375
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..11375
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11375
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[72:2556](-)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(73..2556)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     gene            complement(73..2556)
                     /gene="lanM"
                     /locus_tag="I2I27_RS16800"
     CDS             complement(73..2556)
                     /GO_process="GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lanM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /locus_tag="I2I27_RS16800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="WP_009245993.1"
                     /sec_met_domain="DUF4135 (E-value: 3.3e-97, bitscore:
                     315.5, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 3.8e-19, bitscore:
                     58.1, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVKKMYYLSERIYETMNCEEKQSEARKEYWRNLLGETMFNQMYR
                     ENINFYGYVNGNEYAVRDNVKLKKRFMEFYHSMICLEPNLQIEKKLDKSIEFPRFYGPF
                     LQYGKELFVKKIGKNSDVISEHVYQSLQTYLANILQEVCLRTLIAEMHLYKQQKKLQGK
                     DEREKYEFFHSEIVGTSGFKEELFEKYPVLWRCIEEKIHQAVGYYVEIVENCVDNKIEI
                     QKKFCTGVSIKRITNIKSGLSDVHNQGKSVIVVTLDDSLDLLYKPRSMENELGFLNLLE
                     WISDKVGLDCYKYPILSYDNHSWCSIVKHDSCDTEEQVKRYYQRFGIQLVLVYCLGTRD
                     LHFENVIASGEYPVLVDLEALTYGKREHNTDGIKDAINQHLADSVLCSGLLPYAWQNLN
                     IDSSGISGKGGQKYGFKVPVIVNRRTANMRIEYRYPETKAVQNLVKVKEKDCNPIQHVQ
                     DLLDGFKKAYVKILENKEELLQRSLFLQNLNSRYVTMNTQQYSMLLSASYHPSVMRDGA
                     ERETLFYSLWKGRNETEQEVVDSEIQDLLNGNIPYFSCSVCGKYLIHDGKEISKEYFSK
                     TAWEVFVEKIEKMSVSDMNVQKEYIRMAIELFSGNRCNYENHVYSMDDKKWKERRNQLE
                     KVTIEQLESRILRHAIWNREKTQVNWLTTQLSDQNGANWRLLPMNHYLYSGLAGMLLLF
                     YELKTAKRPQATKVYDTLKNEMFTYTEKGIHSFKDLDSSKTGLYEGEGSIVYVYLCLYK
                     RSNEQIYLRYAEKHSEIVRKLLKQDQRYDLLSGNAGAAWSFLLLYGSTGNTRFLQTGEE
                     AIQILLKSAEVQEQV"
     misc_feature    complement(2638..2654)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.12"
                     /tool="antismash"
     gene            complement(2648..2800)
                     /locus_tag="I2I27_RS16805"
     CDS             complement(2648..2800)
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2I27_RS16805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_009245838.1"
                     /transl_table=11
                     /translation="MADSRVLEMYAGNLLEEMQEIEKMIPGDQENEIVSSHTVGCSAFL
                     TIYCC"
     CDS_motif       complement(2729..2800)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MADSRVLEMYAGNLLEEMQEIEKM"
                     /locus_tag="I2I27_RS16805_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(2648..2728)
                     /prepeptide="core"
                     /locus_tag="I2I27_RS16805_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-5.20"
                     /molecular_weight="2697.0"
                     /monoisotopic_mass="2695.2"
                     /core_sequence="IPGDQENEIVSSHTVGCSAFLTIYCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MADSRVLEMYAGNLLEEMQEIEKM"
                     /leader_location="[2728:2800](-)"
                     /alternative_weights="2715.0"
                     /alternative_weights="2733.0"
     gene            complement(2829..4151)
                     /locus_tag="I2I27_RS16810"
     CDS             complement(2829..4151)
                     /EC_number="2.7.13.3"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /locus_tag="I2I27_RS16810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_009245839.1"
                     /transl_table=11
                     /translation="MLQAFTGKVLLLFLSLWEVWMYYQFLYAVCIEKEQLLKWQRIMIQ
                     ISIWSTGVLMAINRSFLFFSNTMFLFTIILVVLIILLIERKDFILLVSITIVYFSSIAL
                     LDFFFAFFGMCMIEDMFAENIYFSGYQIEKIVIYFCSRSCVACILNGIKKYEVEKSIDR
                     FKYIFLMMGVFFSILVRGYQVIISDMAMGRRELQGIKSLVSMSVVIAVLIFLMILWVKN
                     RVLQEENNTLVMEEQLQHQKYCEMLDVMEHNRELIHDTKHHFLVVQEYLKNEEYENLQK
                     YVKQVSEEFQRTVPKVYTGIKILDFILEQKRVVAQKSGIRYEMDTMLLTGIPMTEQETC
                     ALFGNLLDNAIEACCLVETEEKWIKIQIKQINQLLSIELLNTFEIPCIRKQGVFETIKE
                     ERSAHGYGIKSMQRIVDKYQGLITYEEKEKIFTTKITFFNV"
     gene            complement(4152..4868)
                     /locus_tag="I2I27_RS16815"
     CDS             complement(4152..4868)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="I2I27_RS16815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_009245840.1"
                     /transl_table=11
                     /translation="MIRIGIVDDEEIMRLKIHQCIKEILKEQESVEIRSYDCAEDFLEE
                     VQKKTHFDILFSDIQMLEMNGLELGKRLQKDMPNLYIIYITAYMEYAVESYKISAYQYI
                     LKEDMEIRLPQITRKLLNQIQHEYAQFRVVGTNGIQKKIYFRDIIYVYKGKETKYVYYV
                     TTEGEYKERISLNQVWELLEGNGFLKIERAYLVNAKHILKLEGNKLFLSDQSQLSISRY
                     RLKEVKLDIAKYWGGR"
     gene            complement(4880..5425)
                     /locus_tag="I2I27_RS16820"
     CDS             complement(4880..5425)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2I27_RS16820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_009245841.1"
                     /transl_table=11
                     /translation="MLYNGVEVQTIKLVLSGTESTGISSKQVLLNCIVYLTEKYRESRE
                     FSYLEKAVWHMYAYLELGFSYADGEEIFQTVLDFLCLTREEVFPIQYFGKKVALNKSAI
                     RKILGKWNPKLHSMKVSEAVNDILGNIKNKNTGTYIYHSGKIISKSDNENLWEKTFKLY
                     IQEEAILYDVNKKEYYTF"
     misc_feature    5393..5408
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.96"
                     /tool="antismash"
     misc_feature    complement(5472..5488)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.59"
                     /tool="antismash"
     gene            5555..5878
                     /locus_tag="I2I27_RS16825"
     CDS             5555..5878
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /locus_tag="I2I27_RS16825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_009245842.1"
                     /transl_table=11
                     /translation="MQIGERIRCLRISKGLTQYDLAQAIHISSSFMNRIEKGSSTVSIE
                     YVCSIANALDVTPQDILCDIFVAPETASISEQIKLLSEKLPPDKQLLLLNTLEFIMIQS
                     NLL"
     gene            complement(6007..8274)
                     /locus_tag="I2I27_RS16830"
     CDS             complement(6007..8274)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843186.1"
                     /locus_tag="I2I27_RS16830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_009245843.1"
                     /transl_table=11
                     /translation="MVERRKLPWGLLLSLFVLLMVTAYYLSGLAKLEGVTLENWQNQFV
                     YILLHPLQNWWNDLTFTFLGIALIVWIGIVTYLMDYYRNRQMGVEYGSEQWADLKQIQR
                     NLMNKDQTKNTLLSKNVAVDNGKLSNMNLLILGGSGSYKSTSLVIPNILLASMTNVVLD
                     IKGELLRKTGNYLKEKHIAVKVLNLINPEESDRWNPFVYIRDEVGLVKLITNLQESVKP
                     PDAMKGEPFWDQAVSLYLMSLFSYEWLRQEREKKEHPEQYQAATLPRVLALANLEMQPG
                     SEENSTRLQEKMDDLARRYGPEYPPVRDYRKLKGNMEAQETVSCVILMVNAMLRLCETP
                     AIKRILEADDMEIRSLGTGAENIPGKKTALFLVMPDNDPSFNFLISMFYTTMFDVLIHT
                     ADHECGGALPIHVRLWADEFYAGPKPSKTESLMGTIRGRNMSIVPILQSIAQIKALFQQ
                     DKWEIFVENCAITIYMGSGPGAKSTHKYISELLGEMTIDVRNDGRTFGAHGNSNIQNQK
                     LGRSLMTPAAVKRMSRKHCLIFIEGQYPIFDEKAIPFQTPEWKQMEQLAGKTGYHHPVK
                     VIFDEEQRLYFTLESKKQIQFLNREERDAYQEMAKKEEGIYYREIDREAFLYLNFRKYP
                     KPMEQEIEAQFMQARKERAESVRQTVGAGQSQVPEDVTFFQDLKEKQRFSDVQQYDLSG
                     SILECMERYADQLSIEQKEMIVECLEKGLTEEQIKRLMFRPADEMRNYQRAYLLSNSIG
                     DL"
     gene            complement(8278..9576)
                     /locus_tag="I2I27_RS16835"
     CDS             complement(8278..9576)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843187.1"
                     /locus_tag="I2I27_RS16835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_009245844.1"
                     /transl_table=11
                     /translation="MENERGRQEVLMALQGVGRLGIMMDPVFKLTAAGTILLIQHFARM
                     YKEGLLNRREFQNFQEFAKLTEGNYQIINIPIGSWKELEKAGFVIQMEERGVRYVELPD
                     LNQADGLVQVAIYGEDKLKFQAWYDRFLMAAMKGGEHELRDLNNLTSGRTSIVSIPIEQ
                     NVDLLKDDFDVLQVNYSMLPDLNVGDGDIQVVVANADLAKVEHWYRMYQEQCLTDGKEI
                     PDFHTIDMGKYRETGAMDEESYVDTSSKEIQEAVSKYEGQEPGELEQKVLKQEQSIRSM
                     NNEQYQEFHNNPEFIEITINHETLVQESQLASSWDMERRGMFASRIPGTWGDGEQTLVL
                     PTEQVFRTDDGKTYIGFVERKEKQLILNADGSMVPSEKRSTGERLYAERYEPVSRKFGK
                     KESLQKNTAEKEMIQNVIKQAPSKVPTIPKKGI"
     gene            complement(9579..10118)
                     /locus_tag="I2I27_RS16840"
     CDS             complement(9579..10118)
                     /GO_process="GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015959.4"
                     /locus_tag="I2I27_RS16840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="septation protein SpoVG family protein"
                     /protein_id="WP_009245845.1"
                     /transl_table=11
                     /translation="MKIQVQLYLPDQNQGTVWGYGTITLDQLLTFQIRILTCEKGAGIK
                     EAFVSFPRRKQGERWEDLVIVEDSLRNQITEAVREAIQMEITKDLYLPKIEVLHLQVFP
                     KGKKNFLVGEATIRVLGVTVKGILLKQGKYGVFCHMPQYYSEKKGYQDVIYSPSKRLRD
                     AIFQTVLETYQERQKE"
     gene            complement(10123..10362)
                     /locus_tag="I2I27_RS16845"
     CDS             complement(10123..10362)
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2I27_RS16845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_009245846.1"
                     /transl_table=11
                     /translation="MDQFVKQVLEEEKFSQEQKSYLLDCLEQGEEMEEVLYLAKSCLSV
                     EQMERIKQLLSEHSQMFRCSWRKSWNKKKRDKEG"
     CDS_motif       complement(10249..10362)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MDQFVKQVLEEEKFSQEQKSYLLDCLEQGEEMEEVLYL"
                     /locus_tag="I2I27_RS16845_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(10123..10248)
                     /prepeptide="core"
                     /locus_tag="I2I27_RS16845_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-4.10"
                     /molecular_weight="4889.6"
                     /monoisotopic_mass="4886.5"
                     /core_sequence="AKSCLSVEQMERIKQLLSEHSQMFRCSWRKSWNKKKRDKEG"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MDQFVKQVLEEEKFSQEQKSYLLDCLEQGEEMEEVLYL"
                     /leader_location="[10248:10362](-)"
                     /alternative_weights="4907.7"
                     /alternative_weights="4925.7"
                     /alternative_weights="4943.7"
                     /alternative_weights="4961.7"
     CDS             complement(<10421..11373)
                     /codon_start=2
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /locus_tag="I2I27_RS16850"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="SrtB-anchored collagen-binding adhesin"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAGATYGVFSDKDCTKQLATLTTDENGNTDVAEVTAGTVYIKELS
                     APAGYKVDKTVYPLTIKAGETATLKVSDTPKVTDTLIELFKIDMETQKDNPQGNASLEG
                     AEFTWKYYAGFYNKDNLPAEATRTWVTKTIAETDSDGITHYITKLADAYKVSGDSFYMQ
                     DGKAVLPLGTLTVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSN
                     QFSVSDKVIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNE
                     VVKTIHADIEGVASTSADLLPYGKFRIVESEAPDGYL"
     gene            complement(<10421..>11375)
                     /locus_tag="I2I27_RS16850"
                     /pseudo=""
ORIGIN
        1 tttctcccct acccagttac atatatcagt tcattataaa aagcttagat ttttgtcttg
       61 acaactgaac cactatacct gttcttgcac ctctgctgat tttaataaga tttgaattgc
      121 ttcttctccg gtctgcaaaa acctcgtatt tcccgtactg ccataaagca ataaaaagct
      181 ccacgctgct cctgcatttc ctgataacag atcatatctc tgatcttgct tcagtaattt
      241 tctaacaatt tcggaatgtt tttcagcata tcgtagataa atctgttcat tgcttctttt
      301 atacaaacac aaatatacat aaacgatgga tccctctccc tcataaagtc ctgtcttact
      361 ggaatccaaa tctttgaagg aatgtatccc tttttcagta tatgtgaaca tttcattttt
      421 taatgtgtca tacactttcg ttgcctgtgg tctcttcgcc gtttttagct catagaataa
      481 aagtaacata cctgcaaggc cactatacaa ataatgattc attggcaaaa gtctccaatt
      541 cgcaccattt tgatccgata attgagtcgt taaccaattt acctgtgttt tctcgcgatt
      601 ccatattgca tggcgtaata tcctactttc taattgttct attgtaacct tctcaagttg
      661 atttcttctt tccttccact ttttatcatc catagaatag acatgatttt cataattaca
      721 tcggtttccc gagaaaagtt caattgccat tcgaatatat tctttttgta cgttcatatc
      781 tgaaacgctc attttttcga ttttctcgac aaacacttcc catgccgtct tcgaaaaata
      841 ctctttagaa atttcttttc catcatgtat caaatatttc ccacacactg aacaggaaaa
      901 ataaggaata tttccattta ataaatcttg aatctcgctg tccacaacct cctgttccgt
      961 ttcatttcgc cccttccata atgaatagaa taacgtttct ctttccgccc cgtctctcat
     1021 cacacttgga tgataagagg cagacagcag catactatat tgctgcgtat tcattgtcac
     1081 atatcgacta tttaagttct gcagaaaaag tgacctttgt aataattctt ccttattttc
     1141 caaaattttt acatacgctt ttttaaatcc atccaataga tcctgtacat gttgaatcgg
     1201 attacaatcc ttttctttta cttttactaa attttgaacc gccttcgtct caggatatcg
     1261 atattcgatt ctcatatttg ctgttcttct attaacgatt accggaactt taaagccata
     1321 tttctgccca ccttttcctg aaatcccact actatcgatg ttcaaatttt gccaggcata
     1381 aggaagaagc cccgaacata ataccgaatc tgccaaatgc tgattgatag catcttttat
     1441 tccatctgtg ttatgttccc tttttccata agtcagagct tccaaatcga ccaacaccgg
     1501 atattcccct gatgcaatca cattttcaaa atgcagatct cttgttccca aacaatatac
     1561 taaaacaagt tgtatcccga aacgctgata atatcttttt acttgttctt ccgtatcgca
     1621 gctatcgtgt ttgactatac tgcaccagct atgattatca taagatagga taggatattt
     1681 ataacaatct aatcccacct tatcagatat ccattctaac aaattcaaaa atcccagttc
     1741 attttccata cttcttggct tatataataa atccaaagaa tcatccagtg taaccactat
     1801 cacacttttc ccttgattat gcacatcaga caaccccgat tttatattcg taatcctttt
     1861 tatagaaact cctgtacaaa atttcttctg aatctctatc ttgttatcta cacaattttc
     1921 tactatttca acgtaatatc caacagcttg atggattttt tcttcgatac atctccataa
     1981 aactggatat ttttcaaaca attcttcttt gaaaccactg gttcctacta tctctgaatg
     2041 gaaaaactca tacttctctc tttcatcttt tccttggagt tttttctgct gtttatacag
     2101 atgcatttct gcaatcaatg ttctaaggca tacttcttgc aatatatttg ccaaatacgt
     2161 ctgcaagctt tgatatacgt gctcagaaat cacatcagaa ttttttccta tttttttaac
     2221 aaataactct tttccatatt gtaaaaaagg accataaaat cgtgggaact caatactttt
     2281 atctaatttc ttttctattt gtaagttcgg ttccaggcaa atcatgctat gataaaattc
     2341 cataaaacgt ttctttaatt tcacattatc tctaactgca tactcatttc cattcacata
     2401 tccataaaag ttgatgttct ctctatacat ctgattgaac attgtttctc ccaacagatt
     2461 tctccaatat tcctttcttg cctcagactg tttttcttca caattcattg tttcatatat
     2521 tcgctccgac aaataataca ttttttttac attcatgtct ctcttcctcc gccactctat
     2581 aaacgtacat ataaaatgag cagaatgctt tttcaccaca ctctgcccat aaaaaatttt
     2641 ttatatttta acaacaataa attgtcaaaa atgcactgca ccctacggtg tgtgaagata
     2701 ctatttcatt ctcttgatct cctggtatca ttttttcgat ttcctgcatt tcttccaata
     2761 aatttcccgc atacatttct aacactctgc tatctgccat tttccatttc tcctttgaac
     2821 ttcgatactc acacattaaa aaatgttatt ttagtagtaa atattttttc tttttcctca
     2881 tatgtaataa gcccctgata tttatctact attctctgca tactttttat cccatatcca
     2941 tgtgcgctcc tttcttcttt tatcgtttca aaaacacctt gttttcttat gcaaggtatt
     3001 tcaaacgtgt taagcaactc tattgaaagt aactgattaa tctgtttaat ctgaatctta
     3061 atccattttt cttctgtttc taccaagcaa catgcttcta ttgcattatc taataaatta
     3121 ccaaataggg cacacgtctc ctgctctgtc atcggaattc cagtcaataa catggtatcc
     3181 atttcatatc gaatacctga tttttgtgcc actactctct tttgttctaa gataaaatct
     3241 aagattttaa ttcctgtata aactttaggt actgttcttt ggaattcttc actcacctgc
     3301 tttacatact tctgaagatt ctcatattcc tcatttttaa gatattcctg aacaaccaga
     3361 aaatgatgtt ttgtatcatg gatcaattcc cgattgtgtt ccatcacatc tagcatttcg
     3421 cagtattttt gatgctgcaa ttgttcctcc ataacaagag tattgttttc ttcttgaaga
     3481 actctatttt tcacccacag gatcatcaaa aaaataagca cagcaattac cactgacata
     3541 gaaactaacg atttaattcc ttgcaattct cgtcttccca ttgccatatc tgaaataatc
     3601 acttgatagc ctctcaccaa aatagagaaa aataccccca tcattaagaa tatatattta
     3661 aatctgtcaa tacttttttc tacctcatat tttttaatac catttaaaat acaagcaaca
     3721 caacttctac tacaaaaata gattactatt ttctctatct gatatcctga aaaataaatg
     3781 ttctcagcaa acatatcttc tatcatacac atcccgaaga aagcaaagaa aaaatctagc
     3841 aatgcaatac ttgaaaaata aacaattgtt atacttacca gaagaataaa atcctttctt
     3901 tcaatgagca aaattatcaa tactactaaa atgatagtaa ataaaaacat cgtattagaa
     3961 aaaaataaga agcttcgatt gattgccatt aacacacctg tactccaaat actgatttgt
     4021 atcataattc gttgccattt taataattgc tccttttcga tacatacagc atataaaaat
     4081 tgatagtaca tccatacttc ccacaaggat aaaaacaata ataacacttt tcctgtaaat
     4141 gcttgtagca tctatctccc tccccaatat ttcgcaatat ctaatttcac ctcttttaac
     4201 cgatagcgac taatagaaag ttggctctgg tcacttaaga ataatttatt tccttccaat
     4261 ttcaaaatat gtttagcatt cactaggtac gcacgttcta tctttaagaa tccatttcct
     4321 tctaaaagtt cccaaacctg atttaaagaa atcctctcct tatattctcc ttctgttgtc
     4381 acataataca catattttgt ttctttccct ttatacacat atataatgtc tctaaaatat
     4441 attttctttt gtatcccatt tgttcctaca acacggaatt gtgcatactc atgttgtatt
     4501 tgatttaata atttacgtgt aatctgtggc aagcgtatct ccatatcctc ttttaatata
     4561 tattgatacg cactaatctt atagctttcc accgcatatt ccatatacgc agtaatatag
     4621 atgatataaa gattcggcat atctttttgt aatctttttc ccaactctaa tccattcatt
     4681 tccagcatct gaatatctga aaacagaata tcaaaatgtg tctttttctg gacctcctct
     4741 aaaaaatctt ccgcacaatc atagcttctg atttctactg actcctgctc ttttaaaatc
     4801 tcctttatac attgatgaat cttcaatctc atgatttctt catcatcgac aattcctatt
     4861 cttatcatgt ctattcccct caaaatgtgt agtattcttt cttattcacg tcataaagaa
     4921 ttgcttcttc ttgaatatat aatttaaatg ttttctccca taagttctca ttgtcacttt
     4981 tgctaatgat ttttccagaa tgatagatgt atgtccctgt attcttattt tttatattcc
     5041 ctaagatgtc attcactgcc tccgacactt tcatggaatg taactttgga ttccacttcc
     5101 ctaaaatctt ccgtattgcg cttttattta atgcaacttt ttttccaaaa tattgaatgg
     5161 gaaacacttc ttcacgggtt aaacataaaa aatcaagaac tgtttgaaaa atttcttctc
     5221 cgtctgcata ggaaaatccc aattccaaat atgcatacat atgccaaaca gctttttcta
     5281 aataagaaaa ctctcgactt tctctatatt tctccgttag ataaacaata caattcaata
     5341 gaacttgttt actagatatg cctgtactct ctgttcctga aagaaccaat ttaatggtct
     5401 gaacctcaac accgttatat aacattttac tacaacctcc tttatttcca tagtatctcg
     5461 atatttcgta tttgtaaata tattgcaaat acgtaaattc atttctgtca atactattgc
     5521 taaaatgaaa gaaggaagca aaggaggttt caacatgcaa attggtgaac gtattagatg
     5581 tttacgcatt tccaaaggat taacacagta tgatcttgcc caagctattc atatttcatc
     5641 tagttttatg aaccgaattg aaaaaggttc ctctaccgtt tcgattgaat atgtttgtag
     5701 tattgccaat gccttagatg taacacccca ggacattctt tgcgatattt tcgttgcccc
     5761 agaaacagct tctatttctg aacaaataaa gcttctttct gaaaaacttc cgcctgacaa
     5821 acagctactc ctattaaata ccttagaatt cataatgatt caatcgaatt tattataaca
     5881 atatcttttt attttttaag ctattgacct ttaatagtat atttttagac cttaaacagg
     5941 atattgtatt taatatttat attttctcta tggctattaa atacaatatt cctatttcgc
     6001 tttcaactac aaatccccta tgctattgct caacaaatac gccctctgat aatttctcat
     6061 ttcatccgct ggccggaaca tcaatctttt gatctgctct tctgtcaacc ccttttccag
     6121 acactcgaca atcatttctt tctgctctat actcagctga tccgcatatc gctccataca
     6181 ttccaaaatg ctgcctgaca gatcatattg ttgtacatct gaaaatcttt gcttttcctt
     6241 caaatcctga aagaatgtta catcttctgg aacctgtgat tgccccgctc ctactgtttg
     6301 tctgacggat tctgcccgct cttttcttgc ctgcataaac tgtgcttcaa tctcctgttc
     6361 cattggcttt ggatactttc ggaaattcaa atacaaaaag gcttcccgat caatctcccg
     6421 gtagtagatc ccctcttctt tttttgccat ctcctgatag gcatcccgtt cttcccgatt
     6481 caaaaactga atctgctttt tcgattccag ggtgaaatat aatcgctgtt cttcatcaaa
     6541 aattactttc actgggtgat gatatcccgt cttcccagct aattgttcca tttgcttcca
     6601 ctccggggtc tgaaatggga ttgccttttc atcgaaaatt ggatactgac cttcgatgaa
     6661 gattagacaa tgctttcgac tcattcgttt aaccgccgca ggagtcatca gacttcttcc
     6721 cagtttctgg ttttgaatat tggagtttcc gtgtgcccca aatgtccgcc catcattccg
     6781 gacatcaatg gtcatctccc ccaacagctc cgaaatatat ttatgggtac ttttagctcc
     6841 tggcccagat cccatataga tggtaatggc acagttctcc acaaagattt cccacttatc
     6901 ctgttgaaac aaggctttga tctgcgcaat ggattgcaaa atagggacaa tagacatatt
     6961 ccttccccgg atcgttccca tcaaagattc cgttttactt ggtttgggtc ctgcataaaa
     7021 ttcatccgcc cacagcctga catgaatggg caatgcccct ccacattcat ggtctgccgt
     7081 atggatcagc acatcaaaca tggtggtata aaacatggaa atcaaaaaat taaaagacgg
     7141 atcgttgtct ggcatgacca aaaacaacgc cgtctttttc cccggaatat tctctgcccc
     7201 agttcccaga cttcgaatct ccatatcatc ggcctctaaa attcttttga ttgcaggtgt
     7261 ttcacacagt cgcaacatag cgtttaccat taagattacg caactgactg tttcctgtgc
     7321 ttccatattt cccttgagtt tccggtagtc ccgtaccggt ggatattccg ggccatatct
     7381 tcttgcaaga tcgtccattt tttcctgtaa ccgggtactg ttttcttccg atcctggctg
     7441 catctctaaa tttgccaatg ctaaaacccg tggcaatgta gcggcctggt actgttccgg
     7501 atgctctttt ttctcccgct cttgccgcag ccactcatag gaaaataaag acatgagata
     7561 cagactgacg gcctgatccc aaaacggctc ccctttcatt gcatccggtg gctttacgga
     7621 ctcctgcagg ttagtaatca actttaccag tcccacttca tcccgaatat acacaaaagg
     7681 attccatcgg tcagactctt ccggattgat gaggtttagc acctttactg cgatatgctt
     7741 ctcttttaaa taatttcctg tttttcgcag caattccccc ttaatatcca acacaacatt
     7801 ggtcatagaa gctaacagga tattgggaat aactaatgat gtggatttat aacttcccga
     7861 ccctcccagg atcaaaagat tcatatttga cagttttcca ttatctacag ccacattttt
     7921 gcttaacagt gtattcttcg tttgatcttt attcattaaa tttcgctgaa tctgttttaa
     7981 atctgcccac tgctcggagc catactccac tcccatctgt cggttccggt aataatccat
     8041 cagataggta acaatcccaa tccacacaat cagcgcaatt ccaagaaacg taaatgtcag
     8101 atcattccac caattctgca atggatgcaa caagatgtag acaaactgat tctgccaatt
     8161 ttccaatgtt accccttcca gttttgccag accactcaga taatacgcag taaccatcag
     8221 caatacaaat aaactgagta aaagtcccca tggtaatttt cgtctctcta ccatatctta
     8281 gattcctttc tttggaatgg tcggaacctt ggatggagcc tgttttatta cattttgaat
     8341 catttctttt tctgcagtat ttttctgcag ggattctttc ttaccaaatt ttctggacac
     8401 aggttcatac cgttctgcat acaaccgttc tcctgtactt cttttttcag atggaaccat
     8461 acttccatct gcatttaaga ttagctgctt ctcttttcgt tccacaaatc cgatgtatgt
     8521 ttttccatca tctgttcgaa atacctgctc tgtaggtaac accaacgtct gctctccatc
     8581 tccccaggtt cctggaattc tgcttgcaaa cattccacgc ctttccatat cccagctact
     8641 tgccaattga gactcctgga ccagtgtctc atgattgatt gtgatttcaa taaactctgg
     8701 attattatga aactcctgat actgctcatt attcattgat ctgatcgatt gttcctgttt
     8761 caataccttt tgttctaatt ctcccggttc ttgcccttca tatttgctga cagcttcctg
     8821 tatttctttc gaagacgtat ccacatagga ttcttcatcc atagcacctg tttcccggta
     8881 ctttcccata tcaatagtgt gaaagtctgg aatttctttt ccatctgtca gacactgttc
     8941 ctgatacatt cgataccaat gctccacttt tgcaagatcc gcattggcca ccacaacctg
     9001 tatgtctcca tctcctacat ttaagtctgg cagcatagaa taatttacct gcagaacatc
     9061 aaaatcatcc tttagcaagt ctacattctg ctcaatcgga atactgacaa tactggttct
     9121 tccactggtc aggttgttca gatcccgtaa ctcatgctct ccccctttca tcgctgccat
     9181 taaaaaccgg tcataccagg cttgaaattt caacttatct tctccataaa ttgccacctg
     9241 tacaagtcca tctgcctgat tcaaatccgg cagttctaca taccgaaccc ctcgctcttc
     9301 catttggatg acaaatccgg ctttttctaa ctccttccaa cttcctatag gaatatttat
     9361 gatctggtaa tttccttcgg tcagcttggc aaattcctga aaattctgaa attcccttcg
     9421 gtttaatagt ccttctttat acattctcgc aaagtgctgg atcagcagga ttgttcctgc
     9481 cgctgttaat ttaaataccg gatccatcat aatccccagt ctgccaactc cctgcagtgc
     9541 catcaacact tcctgtcttc ctcgttcgtt ctccatatct actccttttg tcgttcctga
     9601 taagtttcca atacagtctg aaaaatcgca tcccgcagcc gtttggaagg actgtatatc
     9661 acatcttggt atcccttttt ctcactgtaa tattggggca tgtggcaaaa caccccatat
     9721 ttcccttgtt ttaataaaat ccctttcacc gtaactccaa ggacacggat cgttgcttct
     9781 cctacaagga agttcttctt tcctttcgga aatacctgca gatgcaagac ttcgattttg
     9841 ggaagataca aatcttttgt gatttccatc tggattgctt cccggactgc ctctgtgatc
     9901 tggtttcgta gtgaatcctc cacgattacc aagtcctccc atcgttctcc ctgctttctt
     9961 ctgggaaaac tgacaaacgc ctccttgatt cctgctcctt tttcgcatgt caaaatccgt
    10021 atctgaaatg tcagtagctg atccaaggta atggttccat acccccagac tgttccctga
    10081 ttctgatctg gaaggtagag ctgtacctgt atcttcatca cgctatccct ccttgtctct
    10141 tttcttttta ttccaagact ttctccaact gcaccggaac atttgtgaat gctcggaaag
    10201 cagctgcttg attcgctcca tttgttccac agaaagacag gattttgcca gatacaacac
    10261 ttcttccatt tcctcgcctt gttccagaca atccagtaaa taactctttt gctcctggct
    10321 aaacttctcc tcttctaaca cctgttttac aaattgatcc atttgtgcct gtttcctatg
    10381 tagctgtgtt ttccgccgtt ttttcttcca cagtcggttc caagtaaccg tctggagctt
    10441 cactctcaac gatacggaat tttccataag gtaaaaggtc agcagaagta gaagcgacac
    10501 cctcaatgtc tgcatggatt gtttttacca cttcattttt cttgtatagc ttgccctcaa
    10561 ccaataccgc attatcattt aaggaaatga tgtcaaaggc agtatctttc aaagtagcac
    10621 ttccttgtgg ctttgtatcg ccagtttcta aatctcgttt ctgaattttc acaccgccac
    10681 ggataacctt gtctgatacg gaaaactggt tacttccagt taatacggca aggtcgccgt
    10741 cctcggtaat ctgtgtaagg tataagcctt ttatctgttc ggacttatcg ccagcctgca
    10801 tatatgcacc gtctaacaag tagccgtttg gagctttcgt ttcctctacg gttagcgttc
    10861 caagtggaag aactgctttg ccgtcctgca tatagaagct gtcgccagat accttgtatg
    10921 cgtccgctaa ttttgtgatg tagtgggtta tcccgtcact gtcagtttca gcgattgtct
    10981 ttgtaaccca tgtacgagta gcttcggcag ggagattgtc tttattgtag aagccagcat
    11041 aatacttcca tgtaaactct gctccctcta aagaagcgtt cccctgcgga ttgtctttct
    11101 gcgtttccat atcaatcttg aaaagctcaa tcaaagtatc tgttactttc ggcgtatctg
    11161 atacttttaa ggttgctgtt tcgccagctt tgattgtcaa tggataaact gttttatcca
    11221 ctttatatcc tgctggtgcg gataattcct tgatatagac tgtgccagcc gttacctctg
    11281 caacgtctgt atttccgttt tcatcagtcg taagggtggc aagctgtttc gtgcagtcct
    11341 tatcagaaaa gacaccgtaa gttgcaccag cgaca
//
