LOCUS       NZ_JADMPO010000003     21595 bp    DNA     linear   CON 29-FEB-2024
DEFINITION  Mediterraneibacter gnavus strain D52t1_170925_A9
            NODE_3_length_248068_cov_26.9035, whole genome shotgun sequence.
ACCESSION   NZ_JADMPO010000003
VERSION     NZ_JADMPO010000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Mediterraneibacter gnavus
  ORGANISM  Mediterraneibacter gnavus
            Bacteria; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae;
            Mediterraneibacter.
REFERENCE   1  (bases 1 to 248068)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Quantification of discrete gut bacterial strains following fecal
            transplantation for recurrent Clostridioides difficile infection
            demonstrates long-term stable engraftment in non-relapsing
            recipients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 248068)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, 1470 Madison Ave, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 27.07x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/29/2024 03:23:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,493
            CDSs (total)                      :: 3,428
            Genes (coding)                    :: 3,362
            CDSs (with protein)               :: 3,362
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 6 (23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 15 of 66
            Pseudo Genes (incomplete)         :: 56 of 66
            Pseudo Genes (internal stop)      :: 5 of 66
            Pseudo Genes (multiple problems)  :: 9 of 66
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:46:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 226473
            Orig. end                         :: 248068
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADMPO010000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21595
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ranthipeptide"
                     /product="darobactin"
                     /region_number="1"
                     /rules="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085
                     or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /rules="((cds(PF04055 and (TIGR04085 or SPASM)) and
                     darobactin_precursor) or darobactin_rSAM)"
                     /tool="antismash"
     cand_cluster    1..21595
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /detection_rules="((cds(PF04055 and (TIGR04085 or SPASM))
                     and darobactin_precursor) or darobactin_rSAM)"
                     /kind="chemical_hybrid"
                     /product="ranthipeptide"
                     /product="darobactin"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..21595
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:11662](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11662)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
     protocluster    1..21422
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11422](-)"
                     /cutoff="5000"
                     /detection_rule="((cds(PF04055 and (TIGR04085 or SPASM))
                     and darobactin_precursor) or darobactin_rSAM)"
                     /neighbourhood="10000"
                     /product="darobactin"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(10001..11422)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="((cds(PF04055 and (TIGR04085 or SPASM))
                     and darobactin_precursor) or darobactin_rSAM)"
                     /neighbourhood="10000"
                     /product="darobactin"
                     /protocluster_number="2"
     misc_feature    complement(139..152)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.56"
                     /tool="antismash"
     gene            complement(177..1889)
                     /gene="recJ"
                     /locus_tag="I2I27_RS04050"
     CDS             complement(177..1889)
                     /GO_function="GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440042.1"
                     /locus_tag="I2I27_RS04050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="WP_004844384.1"
                     /transl_table=11
                     /translation="MEKWFVSMKKADFTQIAEKYHISPIIARLIRNRDILGDQNIDYYL
                     NGTIADLHDGMLMKNMDTAVEILEEKIREGEKIRVIGDYDIDGVNATYILKTGLESLGA
                     LVDTDIPDRMKDGYGLNQMLIDRALEDGVDTIITCDNGIAAASEIAYGKAQGMTIIVTD
                     HHEVPYLEAGGEKEYLIPDADAVVNPHLPGDPYPFKGLCGAAVVYKVVEALYNVMGQDA
                     DDVDFLMENVAIATVGDVMDLVDENRIFVKQGLEMLKRTQNEGLKALMECTQVPIERLS
                     AYHIGFVIGPCINASGRLDTAKRALELLEVKNRREAVMMAEDLKALNDSRKEMTEKGVE
                     QAVEQIETTDLKEDRVLVVYLQDCHESIAGIIAGRLKEKYYKPVFVLTQGEHGVKGSGR
                     SIESYHMFEEMCKCRALFTKFGGHKLAAGLSLEEENVERFRRTINELCELTSQDLMPKV
                     SIDMQLPLAYVTEELIEELELLEPFGKGNTKPLFAEKNLKVQNLRVIGKNQNVLKFQVL
                     DRNGRKMEAIYFGEVQECMEYLQGKEEVALTFYPSVNEYRGNKSIQITVVNYQ"
     misc_feature    1954..1968
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.5"
                     /tool="antismash"
     gene            complement(2172..7532)
                     /locus_tag="I2I27_RS04055"
     CDS             complement(2172..7532)
                     /GO_function="GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844386.1"
                     /locus_tag="I2I27_RS04055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha-L-fucosidase"
                     /protein_id="WP_242988825.1"
                     /transl_table=11
                     /translation="MKKKALYKKWMSMLLAGVVTTTGFMGGTVTLRAAETTNWVGDEGL
                     SGTADAPEPDKVVPDANQFRYQKEELAAFCHFGPNTFNEIEWGEHYGDKAPSEIFKLKK
                     DFDAETLVNTLKEAGFKKLIVTAKHHDGFCIWDSEHTEYDVKASGYQDAKGESDILAEI
                     SAACTEANMDMGLYLSPWDIHDDSYGYKDKDGKALVEEVTENGHKVNRPIDGHDWNWVY
                     ENDAEDYNKYYQSQLEEILSNPKYGNNGKFVEVWMDGAKGSGSGYQEYTFKEWFETIQK
                     YEGKGVDGRDADCMLFGANDYTTVRWIGNENGIAGKDTWSKSNVDTQKGTIDSNAQGGA
                     TIGFENGNKWTVPEADARITSGWFWGTQKNTPKTMEELSDMYFNSVGHNATLLLNVPPN
                     NQGTVDEAILKRVEEFGKNIKESFDENLAKAEGASVKVSSVRGNAQTYKPGNMIDENDD
                     TYWTTDDGTKSGEILIDLGKETKFDVVSVEEAIQNGQRINNYKIEYRNGDSGAWTLLEE
                     GKTIGAKRLCRTSETTARQIKITVGTTDGKVPMISEVGVYKTTEGMEKANPIPKGMEVI
                     DVTDTDLEDKKGFKFTGKWNPENQPQYINGTNTWANAGAELELKFHGTKAYLFGTLDPG
                     HGTVEITVDDGEKVTVDTKASKRAVGQRWFETPDLEDGDHTIKLKVTGKAVGIEAAAVI
                     NNGGKGMIELESDAYTMNEDETKSLKVKRVGGTKGEIRAKLQPNPGTAIQDDYDTTLIP
                     DIVLGENQTETTADVKTRRNTNKTGDQYFTAEITDVSEGTILGFNKKAKITIKDMESSE
                     GALAALVKECESYQKDWFTSGWDEFELALKQAKAVLEDKNATPEKRNEAEVVLKKAKEG
                     LVKREKYTAEDPFAFPWRKNSSATLEAEFAELHNTGENEKWPLKVSDADWASNKKFVNS
                     LENEDTITIPYHVEKPGIYHTVVTYRSGSETNRFSWSDDAGNIQTGSVSAGNKDSNVTK
                     TAEFDITVTKAGSGVLTLKGDAKAPQLDKFEITPKDVKLSEFTVNASIEGEGGTITPSG
                     ETKVTEGENVEFKITPDKTHKVADVLVNGESVGAVTSYTLKDVKENATVVAKFAPAAYT
                     EENRFNFPTEVNGTAITAEAEHFALKNVGTGEAWPLQVSAADWASNGYFVNAMNSGDQI
                     TLHYHAEKPGKYKATVQFRSGDTNNGLTWSEADNKIAAQTEVMKIGAGDQAKATHTQDI
                     EFVVNEAGDGTLVFTAPEKNAPQLDKFDITLVEEKAPVVVNKDALKAAIDAAKEALKEE
                     DKYTEESVKALKDVVAEAEKVMADEKATQEAVDAAVKEVADAVAGLAEKPAVPEADKTA
                     LKAVLADAAQKLAGADAYTEESVKALKDAVDLAQNVFDNSDASQTEVDAAVTAVRDAIE
                     KLQEKPPVQKEFTITAVAHGGGTIDPSGAVKVEEGKDQVFTIQPYEGFEVKEVFVNGES
                     VGAVTEYKFEAVRADASIEVFFAEKEAVQADKTKLNESIAAAEELLKHAEDYVPEDVQN
                     LMEVLDAAKAVAADPAATQETVDAAQNALDAAMNIAPIQKEFAIMAAAGEGGSITPCGT
                     VKVERGMSQTFVIQPEEGYVISDVLVNGQSVGAVAEYTFCDVNADANITALFQKAAVST
                     DKSQLTAVIKEAEERLEQADKYTEETVKALQKEVEAAQFILADKEASQADIDTAVQKVR
                     TAIDALEEKTQTEKPENPDNNKPDNNKPGNGNNGGQNNNGNSNQSTTQKPSTGTPNKAV
                     KTGDATPIAGAVSGLLAGGAALLAFLKRRK"
     gene            complement(7741..10014)
                     /gene="secD"
                     /locus_tag="I2I27_RS04060"
     CDS             complement(7741..10014)
                     /GO_component="GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA]"
                     /GO_function="GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA]"
                     /GO_process="GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /gene="secD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844387.1"
                     /locus_tag="I2I27_RS04060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="protein translocase subunit SecD"
                     /protein_id="WP_195659686.1"
                     /transl_table=11
                     /translation="MKKSKGIISLILTVVLIALLGFTTVVGFGKGQTGAAKNIKLGLDL
                     EGGVSITYQVKGDTPSKEDMSDTIYKLQKRVEQYSTEATVYQEGDDRISIEIPGVTDAN
                     EILSELGQPGSLYFIKEKDSDGNPNYGLDTSGHYVLAKSMDELKEEGSVVLTGTDIKSA
                     KSGSYQDSTTGANENVVQLSMTKEGTEKFAEATKAAKEAKETIAIYYDGELISVPSVNA
                     EITDGQAIIEGNMSYEEAEQLASTIRIGGLSVELEEIRSNVVGAQLGEEAISTSLMAGA
                     VGLAIVFVFMCVVYLLPGFASSLALVIYTGLILVLLNAFDITLTLPGIAGIILGIGMAV
                     DANVIIFARVKEELTAGKSVKSALNAGFHKAMSAILDGNITTLIAAAVLWLKGSGTVKG
                     FAQTLALGIVVSMFTALVITRMIVYAFYAVGIRNPKLYGRIKEERAPIDFLGKRKIFFA
                     VSVAVILIGFVFMGVNAGTGKGALNYSLEFKGGTSTNVTFDKAYTLKEIDETMIPDLEK
                     VTDDPNIQVQTVANSNQVIFKTQTLDLEKREAFAKYMADEFGVEEKDITTENISSTVSS
                     EMRVDAIIAVAIATVFMLLYIWLRFKDIRFATSAVVALIHDVLVVLAFYVIARISVGNT
                     FIACMLTIVGYSINATIVIFDRIREEMKTKKRGEELDVLVNRCITRTLTRSIYTSLTTF
                     VMVAVLFIIGVSSIKEFALPLMVGIICGAYSSVCITGALWYVMKTKIGKKAEPAKVGTS
                     KKKK"
     gene            complement(10001..11422)
                     /gene="scfB"
                     /locus_tag="I2I27_RS04065"
     CDS             complement(10001..11422)
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="scfB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: Ranthipeptide_rSAM_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     darobactin: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     darobactin: darobactin_rSAM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     darobactin: PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844388.1"
                     /locus_tag="I2I27_RS04065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="WP_022038249.1"
                     /sec_met_domain="Ranthipeptide_rSAM_RRE (E-value: 9.8e-28,
                     bitscore: 90.4, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="PTHR43273 (E-value: 4.2e-77, bitscore:
                     253.7, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 7.3e-16, bitscore: 52.8,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 2.8e-14, bitscore: 47.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 5.9e-19, bitscore:
                     62.4, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="darobactin_rSAM (E-value: 1.0, bitscore:
                     1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIHQYQNNGYHIVLDVNSGSVHVVDKIAYDVIGCLEAMNPAHTPE
                     TLKEEKTAEYLLEKLGDIYAEEDLRDLLEAVAELTAAGQLFTQDVYESYIGEVKERKTV
                     VKALCLHIAHDCNLACKYCFAEEGEYHGRRALMSLEVGKKALDFLVANSGKRRNLEVDF
                     FGGEPLMNWQVVKDLVAYGRELEKTNDKHFRFTLTTNGVLLNDEVQEFVNKEMDNVVLS
                     LDGRKEVNDRMRPFRNGKGSYDLIVPKFQKLAESRNQEKYYIRGTFTRENLDFSEDVLH
                     FADLGFEQISIEPVVGEDTDPYAIQKEDLPKIFAEYDKLAKIMVDREREGRGFTFFHFM
                     LDLEGGPCVAKRLSGCGSGTEYLAVTPWGDLYPCHQFVGQEEFLLGNVEEGITKPEIPE
                     EFRGCSVYSKDSCRNCFARFYCSGGCMANSYKFHHTINDTYEVSCEMERKRVECAIMIK
                     AALADQERMADHEEE"
     aSDomain        complement(11090..11422)
                     /aSDomain="Ranthipeptide_rSAM_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing rSAM protein in a
                     ranthipeptide/SCIFF cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_I2I27_RS04065_Ranthipeptide_rSAM_RRE.
                     1"
                     /evalue="1.00E-28"
                     /identifier="RREFam024.1"
                     /label="I2I27_RS04065"
                     /locus_tag="I2I27_RS04065"
                     /protein_end="111"
                     /protein_start="0"
                     /score="90.4"
                     /tool="antismash"
                     /translation="MIHQYQNNGYHIVLDVNSGSVHVVDKIAYDVIGCLEAMNPAHTPE
                     TLKEEKTAEYLLEKLGDIYAEEDLRDLLEAVAELTAAGQLFTQDVYESYIGEVKERKTV
                     VKALCLH"
     misc_feature    11458..11474
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 23.91"
                     /tool="antismash"
     gene            complement(11519..11662)
                     /gene="scfA"
                     /locus_tag="I2I27_RS04070"
     CDS             complement(11519..11662)
                     /codon_start=1
                     /gene="scfA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: TIGR03973"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: SCIFF"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605085.1"
                     /locus_tag="I2I27_RS04070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="WP_004844389.1"
                     /sec_met_domain="TIGR03973 (E-value: 4.8e-27, bitscore:
                     87.7, seeds: 29, tool: rule-based-clusters)"
                     /sec_met_domain="SCIFF (E-value: 4e-27, bitscore: 88.0,
                     seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKHIKTLNTQTLNHTVKKGGCGECQTSCQSACKTSCTVGNQVCEH
                     KK"
     gene            complement(11746..12291)
                     /gene="spoVT"
                     /locus_tag="I2I27_RS04075"
     CDS             complement(11746..12291)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="spoVT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844390.1"
                     /locus_tag="I2I27_RS04075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="stage V sporulation protein T"
                     /protein_id="WP_055168575.1"
                     /transl_table=11
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTEREGSVI
                     LRKYSPIGEIGAVAGLYAESLAHTMNCIVCLTDMDQIVAASGSGSKELLNRYISKELEY
                     AIQARKQIRTEKRKADYIPIFAGGEEYSQEIICPIISGGDVMGTVVFLHKEEHKKFSEV
                     EEKMAAVAAEFLGRQMEQ"
     gene            complement(12467..12853)
                     /locus_tag="I2I27_RS04080"
     CDS             complement(12467..12853)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612685.1"
                     /locus_tag="I2I27_RS04080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04086 family membrane protein"
                     /protein_id="WP_004844391.1"
                     /transl_table=11
                     /translation="MEKKANVENSMQKKGLWILKSLLCAYLVTGVLLLILTVLLYKCGL
                     EEKHVSAGILTVYILSTLAGGFVAGKMARVRKFVWGLGIGVLYFLLLVLISFGIYHSIQ
                     EPFPQLLIVCILCTGGGMIGGMIS"
     gene            complement(12935..13630)
                     /locus_tag="I2I27_RS04085"
     CDS             complement(12935..13630)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025502.4"
                     /locus_tag="I2I27_RS04085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4304 domain-containing protein"
                     /protein_id="WP_173892250.1"
                     /transl_table=11
                     /translation="MFYKETTGNSAFVVNLTGYNDRVEVVYGFASTAFTFVKGDEESLV
                     RWGIADEDINLRQKSSIFHHAEERDTGILVKEMYDRYKNLEKEALLRIVRIKRKKWIDK
                     IAEKLKPLGFKKKANTWKRVLEDGYYLMFHAQKSSFSDEYYFNVYIGKENTDFYGDCYY
                     NRIVTDCPLDWQTIADDEMIKFLENDIVSQLMHIIDTPLYELGKETMIGEHCECDRQQC
                     VACWIQKKS"
     misc_feature    complement(13749..13765)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 21.4"
                     /tool="antismash"
     gene            complement(13907..15538)
                     /locus_tag="I2I27_RS04090"
     CDS             complement(13907..15538)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244511.1"
                     /locus_tag="I2I27_RS04090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="WP_117636194.1"
                     /transl_table=11
                     /translation="MGITDILSLLGGLALFLYGMHMMSNGLEAAAGNRMKSILEKLTSN
                     RIKGVLVGAVITAVIQSSSATTVMLVGFVNSGLMTLSQAVWVIMGANIGTTITGQLIAL
                     DISAIAPIFAIGGVAAMMFIKNEKVHHISGIFSGLGILFMGMAMMGDAMVPLQDSQTFI
                     NFMTTVENPLVGILIGAIFTAIIQSSSASVGILQALAATGMVPLSSAVYILFGQNIGTC
                     ITAVLASIGMKVNAKRTTVIHLMFNIFGSILFTVICMTTPFVSWMEALTPGNPVAQIAN
                     VHTTFNIVTTLILLPFGNVMARIATQILPDSKKEDDGDYRLKYITRFESNYAIGSSAVA
                     LSQVRDEIERMRDMVSKNIAKAYDVLIQYNEKDMEKISERETYIDYLNREISEYIVSLI
                     SNEKSTEDSKIINGYYAVIGNLERIGDHAMNLAGYAKDLKEWNLSFSDVALEEIEEMKK
                     QCLTALDIVKNEEGSDMTQVLFEASAAEQKIDDLRDKYFKKQMQRMKKGKCKPQSGIIF
                     TEMLTDFERMGDHVKNIAQQYKQMGE"
     misc_feature    15687..15702
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.55"
                     /tool="antismash"
     misc_feature    complement(15687..15704)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.94"
                     /tool="antismash"
     misc_feature    15812..15826
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.05"
                     /tool="antismash"
     gene            15842..16288
                     /locus_tag="I2I27_RS04095"
     CDS             15842..16288
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844394.1"
                     /locus_tag="I2I27_RS04095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="WP_073963088.1"
                     /transl_table=11
                     /translation="MSKIDEVRSAMMAAMKAKDKERKDALSALLTALKNKAIDKRADLT
                     EEEETQVILKEIKQLKETIEMTPADRTDILAECNSRLAVLEEYAPKMMDEAEIKAVVSE
                     VLTSLGLDAPTAKEKGKIMKELMPKVKGKADGKLVSEVVASFLS"
     gene            16295..17449
                     /locus_tag="I2I27_RS04100"
     CDS             16295..17449
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_tran_28_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844395.1"
                     /locus_tag="I2I27_RS04100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase"
                     /protein_id="WP_004844395.1"
                     /sec_met_domain="Glyco_tran_28_C (E-value: 7.7e-08,
                     bitscore: 26.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVLILSCNTGEGHNYAGRALKECIESHHDTADMLDIMMLASPRV
                     SKLVGNSYVNIVRHAPRLFQCLYKLGGLVSSARHHSPVYYANALLAKKLTRYLDTHHYD
                     VIVTPHLFPAQTLTYIKKKNLLSQKVVAVETDYTCIPFWEETDCDYYIIPHYELIDEFT
                     AKGIPRERLKPYGIPVRPAFSDQSDRQKARVRCGIPTHAQVYLIMSGSMGFGKIQLFVA
                     ELLRTRKPGEYVVVICGNNRRLQKILLAEFGKQEGVQILGYTEKIADFMAAADVLFTKP
                     GGLTTTEAAVKGIPIVHTRPIPGCETKNLAFYTERGLSLTSQKLHGQILAGRKLMSNDE
                     LRHSMCTAQHTVIPPNAAEKTYQLLCQLSSAYAVSDDTAKKETL"
     gene            17446..18048
                     /locus_tag="I2I27_RS04105"
     CDS             17446..18048
                     /EC_number="2.3.1.275"
                     /GO_function="GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /GO_process="GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844396.1"
                     /locus_tag="I2I27_RS04105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="WP_004844396.1"
                     /transl_table=11
                     /translation="MNELFFILVGYLSGSILYAYLLPKYICHIDIMKDSDDHNPGTFNA
                     FALAGTQVGILVIALELLKGFLPIWLASHILDTRRWMFAFVLCAPVAGHAFPLFYPKRG
                     GKAIAVSFGVLLGLLPRYRPVLLLIFFYLLFSFLIVVKPHLYRSILTFTLFSLTGLFYF
                     HDAVISMGTFFISCVVIIRHLVHHQKEPFSIRFLQRS"
     gene            complement(18060..18611)
                     /locus_tag="I2I27_RS04110"
     CDS             complement(18060..18611)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844397.1"
                     /locus_tag="I2I27_RS04110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="folate family ECF transporter S component"
                     /protein_id="WP_004844397.1"
                     /transl_table=11
                     /translation="MKQLKQQFVDSWHELRKTKVMAVAAMLIAIGVILGFFSVQLTEFI
                     RIGFSGIPNELASMLFGPVVGGIMGGIGDILKFLIKPTGPYFFGYTLNAMLGPVIYGIF
                     FYHRPIQLGRVVAAKITVALLVNLLLGTWWLTILYGKGFLAILPARFIKQVVSVPIDSV
                     IFYVLAKTLERSKAISMIKK"
     regulatory      complement(18694..18792)
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            complement(18844..19623)
                     /locus_tag="I2I27_RS04115"
     CDS             complement(18844..19623)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844399.1"
                     /locus_tag="I2I27_RS04115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SseB family protein"
                     /protein_id="WP_118013404.1"
                     /transl_table=11
                     /translation="MKEENKNGQLPEKEQLLSSLRNAEEMYVFMSLCTKMPYVLCDEET
                     FDDEVLLYYTEEDAQREGKKLIEQRIPIQIAKIEKKQLLGFFSSLYPMGVNGLLINKNM
                     ESEARLQLGELVIRPNTGDLPDGKVWVENPQLHLTALYFMQEMRRQEKPELTEELKGLQ
                     EEILVNYGRGRFIVAVHKENGMPMLKQKNGDAYQPIFTDILEFRKFNKEDQFKTMAIEA
                     KNVPKMLVNEAKGVVINPYGVNLQIPIVRPEEPQEKK"
     gene            complement(19706..20536)
                     /locus_tag="I2I27_RS04120"
     CDS             complement(19706..20536)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844400.1"
                     /locus_tag="I2I27_RS04120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_009244512.1"
                     /transl_table=11
                     /translation="MNQERYIKTGEFAKLVGVTKHTLFYYDKIGLFSPEIKLENGYRFY
                     SFDQLDVFDVIQTLRELDVSLEEIKGYMNQRSPKRLLKLFRKEQSIIRKQMQQLKKMEE
                     WIVKKSEIIEKTMQTDTEAIRIEEEPDRYMIQSCATDTNERVWAEKIGELFEYCARNGI
                     KSAYSIGYRQNTKEIQSGIFDQYSVFYELLDEKPQKVNYSVRPAGMYLIAYHKGKWQTL
                     EDTYKKILEYGKENKIQLGAHCYEDILFDSLTMSEEEEYLTRIVFEIQSSKSGK"
     gene            complement(20715..21500)
                     /locus_tag="I2I27_RS04125"
     CDS             complement(20715..21500)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844401.1"
                     /locus_tag="I2I27_RS04125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF368 domain-containing protein"
                     /protein_id="WP_243271760.1"
                     /transl_table=11
                     /translation="MLIGLGAVLPGISGGVLCVIFGIYRPVMELLSNPRKYFFTHIRKL
                     LPVILGGAVGFLGVANILSFFLEKYPDPSVCLFVGLIAGMLPSLMREAGKEGRTNGSWI
                     SMVAAAVVIFAILTTLNLLSVSLVPNVGWYVFCGVCLALSVIVPGMSFSTLLMPLGLYT
                     PFVDGLGHLNLSIMIPSGIGAVVTVICFAKMIDALLEKFYSYVFHAIVGIVVAATVMII
                     PFGSFTVSLSAAIVNVICLGLGVLCSLLLNRIDKDDLLA"
ORIGIN
        1 tgcaatcaac gcttcatata actgctcagg gttttgataa tcttcaggtg ctttcacggc
       61 atggccatcc accatttcca gatttttatt cagaagttca tattccagcg tacctgccat
      121 atcaaatccc ccttttcttt ttatcgtaaa attccatatg gattagtata tcatcattat
      181 tgataattta ccactgtaat ctgtatgctt ttgtttcctc tgtattcatt gaccgacgga
      241 taaaatgtca gcgccacctc ttcttttccc tgcagatact ccatacactc ctgtacctct
      301 ccgaaataga tcgcctccat tttccgtcca tttcgatcca gaacctgaaa tttcaggaca
      361 ttctgattct tcccgatcac acggagattc tgtactttta agtttttttc cgcaaacaac
      421 ggctttgtat ttcctttccc gaaaggctcc agaagttcca gttcttcaat cagctcttct
      481 gttacatacg caagaggaag ctgcatgtca atggatacct tcggcatcag atcctgactg
      541 gtcagctcac agagttcatt gatcgttctg cggaaacgtt ccacattctc ctcttccaga
      601 gatagccctg ccgccagctt atggcctccg aattttgtga ataacgccct gcatttgcac
      661 atttcctcaa acatatggta gctttcaatg gaacgccccg accctttgac gccatgttct
      721 ccctgcgtca gaacaaacac cggcttataa tatttttctt tcagtcttcc ggcaatgatc
      781 cccgcaatac tctcatgaca gtcctgcaga tacacgacca gtactcgatc ctctttcaaa
      841 tccgttgtct cgatctgttc aactgcctgc tctacccctt tttctgtcat ttccttacgg
      901 ctgtcattca atgcttttaa atcttctgcc atcatgacag cttctcttct gttcttcacc
      961 tcaaggagct ccaatgcccg ctttgccgta tccagtcgcc cgctcgcatt gatacatggt
     1021 ccgatgacaa atccgatgtg ataggcagac aatctctcta tcggtacctg cgtgcattcc
     1081 atcaatgctt tcagaccttc attttgcgtg cgtttcagca tttccagccc ctgttttaca
     1141 aaaatccggt tctcatccac cagatccatc acatccccca cggtcgcaat cgccacattt
     1201 tccatcagaa aatctacatc atctgcatcc tgccccatga cattatataa cgcttccacc
     1261 actttataca caactgccgc accgcacaga cctttaaacg gataaggatc tcccggtaaa
     1321 tgcgggttga ccactgcatc tgcatcggga atcagatatt ctttctctcc tcctgcttct
     1381 agataaggta cttcatggtg atctgtcaca atgatcgtca tcccctgcgc tttcccgtaa
     1441 gcaatctctg aagcggctgc gatcccattg tcacaggtaa tgatcgtatc tacaccgtcc
     1501 tccaatgccc tgtcaatgag catctgattg aggccgtatc catccttcat ccgatccgga
     1561 atatccgtat ccaccaaagc tcccagactt tctaatcctg ttttcaaaat atacgtcgca
     1621 ttgactccgt cgatatcata atctccgatc acacggattt tttcgccttc ccggattttt
     1681 tcctccagaa tctccactgc cgtatccata tttttcatca gcatcccatc gtgcagatct
     1741 gcaatcgttc catttaaata atagtcaata ttctgatcgc ctaaaatatc cctgtttcgg
     1801 atcaggcgcg cgatgatcgg ggaaatatga tatttttccg caatctgcgt aaaatccgct
     1861 ttcttcatgg acacaaacca cttttccatg tcagttcctc ccaagtatct ttcttgtttt
     1921 tctagctgtt aatttttatt gtagcatact aaaaattttc tgtaaatctg tccagaactg
     1981 gagtgcgcaa gcacgacgat tgcgcatgga taggtgggga atggtgtgaa cggtaacaag
     2041 aactgttctg ccagcgaata ttttattcaa acatcttccg ccaaagccaa atttcccgta
     2101 ccataaaaca gtgccccatg cctttcggca cagagcactg ctcatgttta ggaagaattt
     2161 tttattttta tttatttgcg tcttttcagg aatgccagaa gtgctgctcc tcctgcaaga
     2221 agtccggata ctgctccggc aatcggagtg gcatctcctg tcttcactgc tttgtttgga
     2281 gtaccagttg acggtttctg tgttgtagac tgattgctgt ttccgttatt gttttgtccg
     2341 ccattgtttc catttcccgg tttgttgtta tccggtttat tattgtctgg attttctggt
     2401 ttttcagtct gcgttttttc ctccagtgca tcgatcgcag ttcttacttt ctgtactgct
     2461 gtatcaatgt ctgcctgaga tgcctctttg tctgcaagaa taaactgcgc cgcctccact
     2521 tctttctgaa gcgcttttac agtctcttct gtgtacttat ctgcctgctc cagacgttcc
     2581 tctgcttctt ttataacagc tgtaagctgt gatttatctg tagaaacagc tgctttctgg
     2641 aagagtgctg taatattggc atctgcattg acatcacaga atgtatactc tgccactgca
     2701 ccaacagact gtccatttac cagtacatca ctgatcacat atccttcttc cggctggatc
     2761 acaaatgtct ggctcatacc gcgttctact tttacagtac cgcatggagt gatgcttccg
     2821 ccctctcctg ctgctgccat aattgcaaac tctttctgga tcggtgcaat attcattgct
     2881 gcatccagtg cattctgtgc cgcatctact gtttcctgtg tcgctgccgg atctgctgct
     2941 accgctttcg ctgcatccag tacttccatc agattctgaa catcttccgg aacatagtcc
     3001 tctgcatgtt tcagaagttc ttctgctgct gcaatggact cgttgagttt tgtcttgtca
     3061 gcctgtactg cttctttttc tgcgaagaat acttcaatgc ttgcatctgc acgaactgcc
     3121 tcaaatttgt actctgtaac agctccaacg gattctccat ttacaaatac ttcttttact
     3181 tcaaatcctt catatggttg gattgtaaat acctgatctt ttccttcttc tactttcact
     3241 gcaccgcttg gatcaatggt tccaccgcca tgtgcaactg ctgtgatcgt aaattctttc
     3301 tgtaccggtg gtttttcctg caatttttca attgcatctc gtacagctgt cacagctgca
     3361 tcaacctctg tctggctcgc atccgaatta tcaaatacat tctgcgcaag atctactgcg
     3421 tctttcagcg cttttacaga ttcttctgtg taagcatctg ctcccgcaag tttctgagcc
     3481 gcatctgcaa gaactgcttt cagggctgtc ttatctgctt ctggtactgc cggtttttct
     3541 gccagacctg ctactgcatc cgctacctct tttactgctg catctaccgc ttcctgtgtt
     3601 gctttctcat ctgccattac tttttctgct tctgctacta catctttcag ggctttgaca
     3661 gactcttctg tatacttatc ctcttctttc agcgcttctt tggctgcatc gattgctgct
     3721 ttcaatgcat ctttgttcac tacaactggc gctttttctt ctaccagagt aatgtcaaat
     3781 ttatcaagct gtggcgcatt cttttccggt gctgtaaata ccagtgttcc atcacctgct
     3841 tcatttacta caaactcgat atcttgtgtg tgtgttgctt ttgcctgatc tccagctcct
     3901 attttcataa cttctgtctg agccgcaatt ttattatcag cctcagacca agtcaatcca
     3961 ttatttgtat caccgctacg gaactgtaca gttgctttat atttccccgg tttttctgca
     4021 tgatagtgta aagtaatctg atcaccacta ttcattgcat ttacaaagta tccattgctt
     4081 gcccaatcgg ctgcagacac ctgtaatggc catgcttcac ctgttcctac attctttaat
     4141 gcaaaatgct ctgcctctgc tgtgattgct gttccattta cttctgtagg gaaattgaaa
     4201 cggttttctt ctgtatatgc tgccggagca aatttagcaa caaccgttgc attttccttc
     4261 acatctttta atgtataaga tgtgacagct cctacagatt caccatttac cagaacatcc
     4321 gctaccttat gtgtcttgtc tggtgtgatc ttgaattcta cattttcacc ttctgttact
     4381 tttgtttctc cactcggtgt aattgtaccg ccttcccctt cgatggatgc attgactgta
     4441 aactcagaca atttcacatc ttttggtgta atttcaaact tatctaactg tggtgctttc
     4501 gcatcaccct tcaatgtcag cacaccgctt cctgctttag taaccgtaat gtcaaattct
     4561 gctgtctttg tcacattcga atctttgttt cctgctgata cagatccggt ctgaatattt
     4621 ccagcatcat cactccaaga gaatctattt gtctctgaac cgcttctata agttactact
     4681 gtatgataaa ttcccggttt ttctacatga taaggaattg taattgtatc ctcattttcc
     4741 aaagaattca caaatttttt attgcttgcc caatctgcat ccgatacttt taatggccat
     4801 ttttcattct cacccgtatt atgtaattct gcaaactctg cctctaatgt agcactggaa
     4861 ttctttctcc atgggaatgc aaacggatct tccgctgtgt atttttcacg ttttactaat
     4921 ccctcttttg cttttttcag aactacttct gcttcatttc tcttttctgg agttgcattt
     4981 ttatcttcta aaacagcctt tgcctgtttc agcgctaact caaattcatc ccatccagaa
     5041 gtaaaccagt ctttctgata agattcacat tcttttacaa gcgcggccaa tgcaccttca
     5101 ctgctttcca tatctttgat ggtgatttta gctttcttgt taaacccaag gatcgttcct
     5161 tcactaacat ctgtaatttc tgctgtgaaa tattgatccc cagttttatt tgtatttcgt
     5221 cttgttttaa catctgctgt tgtttctgtc tgattttctc ccaatacaat atccggaatt
     5281 aaggttgtat cgtaatcatc ctgaattgcc gtgcctggat tcggctgcaa ttttgctctt
     5341 atttctcctt tcgttccacc aacacgtttt acttttagag attttgtttc atcttcattc
     5401 atggtgtatg catctgattc taactcaatc atacctttcc caccattatt gataactgca
     5461 gcagcttcaa ttcctactgc ttttccagta accttcagtt taatcgtatg gtctccatct
     5521 tccagatccg gagtttcaaa ccaacgttgt cccactgcac gctttgatgc ttttgtatca
     5581 actgtaactt tttcaccatc atcaactgta atttctaccg ttccatgacc gggatctagt
     5641 gttccaaaaa gatatgcttt tgttccatgg aatttcagtt ctaattctgc ccctgcattt
     5701 gcccatgtgt tcgttccatt aatgtactgc ggttgatttt ccggattcca ttttcctgta
     5761 aatttaaatc cttttttatc ttctaaatct gtatctgtca catcaattac ttccataccc
     5821 tttggaatcg gattggcttt ttccatacct tctgtagttt tatacactcc aacttcacta
     5881 atcattggaa cctttccatc tgttgtccct actgtgattt taatctgacg cgctgttgtc
     5941 tcgctcgttc tacaaaggcg tttcgcacca atagtttttc cttcttccaa caaagtccat
     6001 gcaccactgt ctccattgcg atactctatc ttgtaattat taattctctg accgttctga
     6061 attgcctcct caactgatac aacatcaaac ttcgtttctt ttcctaagtc tattaaaatt
     6121 tcacctgact ttgttccatc atctgtcgtc caatatgtat cgtcattctc atcaatcata
     6181 ttgcctggtt tgtaggtttg agcattcccc ctgacagaag atacctttac agatgcccct
     6241 tctgcttttg ccaggttttc atcaaatgat tctttgatat tcttaccaaa ctcttctaca
     6301 cgtttcagaa tcgcttcatc tactgttccc tgattatttg gtggcacatt taacagtaat
     6361 gttgcattat gtccgacaga attgaaatac atatcgctca gttcttccat cgtttttgga
     6421 gtgtttttct gtgttcccca gaaccatcct gatgtgatac gagcatctgc ttccggaacg
     6481 gtccacttat ttccattctc aaacccaatt gtagcaccac cttgagcatt gctatctatt
     6541 gtaccctttt gtgtatccac attagatttt gaccaggtat cttttcctgc tataccattt
     6601 tcatttccaa tccagcgtac agttgtataa tcatttgctc caaacaacat acaatctgca
     6661 tcccgtccgt ctactccttt accttcgtat ttctgaattg tctcaaacca ctctttaaag
     6721 gtatattcct gatatccaga gccactgccc ttcgctccat ccatccatac ttctacaaat
     6781 tttccattat ttccatattt aggattactt aaaatttctt ctaactgtga ttgatagtat
     6841 ttattataat cttctgcatc attttcataa acccagttcc agtcatgtcc atcaattggt
     6901 ctattcacct tgtgaccatt ttctgttact tcttcaacca aagccttccc atctttatct
     6961 ttatatccat aactatcatc atgaatatcc catggtgaca aatacaagcc catatccata
     7021 ttagcttctg tacatgccgc agagatttct gctaatatat cagattcacc ctttgcatcc
     7081 tgatatccag atgcttttac atcatattct gtatgttcac tgtcccagat acagaatcca
     7141 tcatgatgtt ttgcagtcac aatcaacttt ttaaatccag cttctttcaa tgtgttgact
     7201 agggtttctg catcaaaatc ttttttcaat ttaaaaattt cacttggtgc tttatcccca
     7261 taatgttctc cccattcaat ctcattgaat gtattcggtc caaaatgaca gaatgctgca
     7321 agttcctctt tctgatacct aaattggttt gcatccggca ctaccttgtc cggttctgga
     7381 gcgtccgctg ttccgctcag tccttcatct ccaacccaat tcgttgtttc tgctgctctt
     7441 agtgttaccg ttccccccat gaacccggtc gttgtcacta ctcctgcaag cagcattgac
     7501 atccactttt tgtacaaagc ctttttcttc accttttttc tcctttctga cataccatat
     7561 ttatatatgg gattatactc cctcacttaa attttcatca attcattatt ttccaatttt
     7621 tggttaaaat tttccagttc tgggtaaatt tttaactatt ttcaccatga tgaacaaaag
     7681 aacccttgga ttatgtgtct gttttccaca taaaccaagg gttctctcac ttacttttcg
     7741 ttattttttc ttttttgatg ttccaacttt tgcaggttct gcttttttcc cgatcttcgt
     7801 cttcatcaca taccacagcg cacctgtaat acatacagaa gaatatgctc cacagataat
     7861 acctaccatc agagggagtg caaattcttt aatggagctt acacctataa taaacagcac
     7921 cgccaccata acaaatgttg tcagagatgt ataaatactt cttgtcagag ttcttgtaat
     7981 acatctgttt accaggacat ccaactcttc tccacgtttc tttgtcttca tctcctcgcg
     8041 gattcggtca aaaataacga ttgttgcatt gatagaatat ccgacaattg tcagcataca
     8101 tgcaataaat gtatttccta ctgaaattct tgcgatcaca tagaaagcaa gtacgaccag
     8161 tacatcatga atcagtgcaa ctactgcact tgtcgcaaac cggatatctt taaaccgcag
     8221 ccagatataa agcagcataa agactgtggc aatggcaacc gcaatgattg catccacacg
     8281 catctcggag cttacggtag aactgatgtt ttcagtcgta atatcttttt cttctactcc
     8341 gaattcatct gccatatatt tcgcaaatgc ttctcgtttt tccagatcca gcgtctgtgt
     8401 cttgaaaatg acctgattac tgtttgcaac ggtctgtacc tggatgtttg gatcatctgt
     8461 caccttctcc agatccggaa tcattgtttc atcaatctct ttcaacgtat atgctttgtc
     8521 aaatgttaca tttgtagatg taccaccctt aaattccaga ctgtaattga gggcgccttt
     8581 tcctgttcct gcattcactc ccataaacac aaatccaatc aggatcactg cgaccgatac
     8641 tgcaaagaaa atcttacgtt ttccaaggaa atcaatcggc gcacgctctt ctttaatccg
     8701 tccgtaaagc tttggattac gaattccaac tgcatagaac gcatacacga tcatccgggt
     8761 gatcacaagt gcggtaaaca tggataccac aataccaagt gccagtgtct gtgcgaatcc
     8821 tttgactgta ccgcttcctt tcagccagag cactgctgcc gcgatcagtg ttgtaatatt
     8881 tccatccaaa atcgcagaca ttgctttgtg gaatccagca tttaatgcag attttacaga
     8941 ttttccggct gtcagttcct cttttacacg cgcaaagata atgacattcg catcgaccgc
     9001 cataccgata ccaagaatga tacccgcgat acccggcagc gtcagtgtga tatcaaatgc
     9061 attgagcagc accaggatca gtccggtgta gattaccagt gcaagactgg atgcaaatcc
     9121 cggcagcaga tataccacgc acataaatac aaatacaatc gcaagcccga ccgcacctgc
     9181 catcagactg gtactgattg cttcttcacc aagctgtgct ccaactacat tagaacgaat
     9241 ctcttccagt tctacactca atccaccaat acggatcgtg gatgccagct gctcagcctc
     9301 ttcatatgac atatttcctt cgattatcgc ctgaccatct gtgatctctg cattcaccga
     9361 tggaacactg atcagttctc cgtcataata gatcgcaatc gtctcttttg cttcctttgc
     9421 cgctttcgtt gcctctgcaa acttttcagt accttccttt gtcatagaaa gctgaacaac
     9481 attttcattt gctcctgttg tggaatcctg ataagaaccg gactttgcac tcttgatatc
     9541 tgttccggtc aaaacgacag atccttcttc ctttaactca tccatacttt ttgcaagcac
     9601 atagtgtcct gaggtatcca gtccataatt cggatttccg tcactgtcct tctctttgat
     9661 aaaatacagg gatcccggct gaccaagctc gcttaaaatc tcgttggcat ctgtcacacc
     9721 tggaatctct atgctgatgc ggtcatctcc ctcctggtaa accgttgctt ctgtactgta
     9781 ctgttctaca cgcttctgaa gcttgtagat tgtatcactc atatcctcct tggaaggagt
     9841 atcccctttt acctgatagg taatactgac tccgccttcc aggtcaagtc caagcttgat
     9901 attctttgct gcccccgtct gtccttttcc aaatccgacc accgttgtaa atccaagaag
     9961 tgcaatcagc accacggtca gaatcagact gataatccct ttactcttct tcatgatctg
    10021 ccatcctctc ctgatcagcc agtgccgcct tgatcataat cgcacattcc acacgcttgc
    10081 gctccatctc acaacttacc tcgtatgtat cattgatcgt atgatggaat ttataagaat
    10141 tcgccataca tccgccgctg cagtaaaatc ttgcaaagca gtttctgcag ctgtctttgg
    10201 aatacacact gcatcctctg aattcttccg gaatctccgg ctttgtaatt ccttcctcta
    10261 catttcccag caggaattct tcctgaccaa cgaactgatg acacggatac agatctcccc
    10321 aaggcgtaac tgccagatat tctgttccgg aaccacatcc ggacagacgt tttgccacgc
    10381 aaggaccgcc ttctaaatcc agcataaagt ggaagaacgt aaaccctctg ccttcccgtt
    10441 ctctgtctac catgattttt gccagcttat catactctgc aaaaatcttc ggaagatctt
    10501 ctttttgaat cgcatacgga tctgtatctt ccccgactac aggctcaatt gaaatctgct
    10561 caaatcccag atctgcaaaa tgcaggacgt cctctgaaaa gtccagattt tctcttgtaa
    10621 atgttcctcg tatataatac ttttcctgat ttctgctctc tgccagtttt tggaatttcg
    10681 gaacgatcag atcataactt ccttttccat tcctaaacgg acgcattctg tcgttgactt
    10741 cttttcttcc atccagactg agcaccacat tgtccatctc tttattgaca aattcctgca
    10801 cttcatcatt gagcagaact ccatttgttg tcaatgtgaa acggaaatgc ttgtcgtttg
    10861 tcttctccag ctctcttcca taggcaacca gatctttcac tacctgccag ttcatcaacg
    10921 gctctccgcc aaagaaatca acctcgagat ttcttctctt tcctgaattt gccacaagga
    10981 aatccagcgc tttctttcca acttccaaag acatcagcgc acggcgtcca tgatactcac
    11041 cctcttccgc aaagcagtat ttgcaggcaa gattacaatc atgcgcaatg tgcaggcaaa
    11101 gtgccttaac aactgtcttt ctttctttta cttctccgat atagctctca taaacatcct
    11161 gtgtaaacaa ctgacctgcc gctgtcagct ctgccactgc ctccagaaga tctctcaaat
    11221 cttcttctgc ataaatatcc ccaagctttt caagcagata ttctgccgtc ttctcttctt
    11281 ttaacgtttc cggcgtatgg gcagggttca tggcctccag acagccgatc acgtcatacg
    11341 caatcttgtc cacgacatgc acagatccgc tgttcacatc caggacaata tgatatccat
    11401 tattctggta ctgatgaatc acaacagtta cccctctttc atttaaattt tatatttttg
    11461 cataacacac gtaaggcagc gaccacggcc gctgccctca tgtctgatat tcagtttctt
    11521 attttttgtg ttcacaaacc tgatttccta ctgtacagga tgtcttacat gctgactgac
    11581 aagatgtctg acattctccg catccacctt ttttcactgt atgattcaat gtctgagtat
    11641 ttaatgtttt gatatgtttc atttctattc ctcctaatgt gtatgcataa gcatctagtt
    11701 ttcttagaat tataacacat cctgatcact ttttaaagta tttttttact gttccatctg
    11761 tctgcccaga aattctgctg ccacagctgc cattttttct tccacttcac tgaatttttt
    11821 gtgttcttct ttgtgtaaaa acaccactgt tcccatcacg tccccgccac tgataatcgg
    11881 acaaataatt tcctgagaat attcctcacc cccggcgaat atcggaatat agtccgcttt
    11941 ccttttttct gtacggatct gctttcgagc ctgtattgca tactccagtt ctttactgat
    12001 ataacggttc agcagctcct tgctcccact tcccgatgct gctacaatct gatccatatc
    12061 agtaagacac acaatacagt tcatggtatg tgcaaggctt tctgcgtaca gtccggcaac
    12121 agcaccgatt tctccgatcg gagaatactt tcttaaaatc acactgcctt ctctctcggt
    12181 aaaaatttcc agtggatctc cttcccgaat acgaagtgtc cttcgaattt cttttggaat
    12241 caccaccctg ccaaggtcat caattcttcg tacaattcct gtcgctttca tcttatcttc
    12301 tccttatatc tggtttttat cgataacaat ctgctattag tatcgctcat tttttagaaa
    12361 ttatgagtta ttttaccagt tttataagaa ttcatgaggg atgtattttt tccgaaaaga
    12421 gtaactacaa gagattttct atcgttctct ctgtacgttt acttcctcac gaaatcatac
    12481 cgccgatcat tccacctcct gtgcaaagaa tacacacgat caaaagctga ggaaacggct
    12541 cctgaatgct gtggtaaata ccaaaggaaa tcaatacaag aagcagaaaa tacagcacac
    12601 cgattccaag cccccagaca aatttcctga cccttgccat tttccctgct acaaaaccac
    12661 ctgccagtgt agacaaaata tacacggtca aaatccctgc tgaaacatgt ttttcctcca
    12721 gtccacattt gtaaagcagt actgtcaaaa tcaaaaggag cacacctgtt acaaggtatg
    12781 cacacaacag ggattttaaa atccaaagtc cttttttctg cattgaattt tcaacatttg
    12841 ctttcttttc catatacgct ctcctcccgg ctcatttaca gctaatacac tgtatgagtc
    12901 cgaaaagcgt tttatgtcca tcgttccatt ggtatcagct ttttttctgt atccagcagg
    12961 caacacattg ttgtctgtca cactcacaat gttcccctat cattgtctct tttcccaact
    13021 catatagcgg tgtgtcaatg atatgcatca actgactaac aatatcattc tctaaaaatt
    13081 ttatcatttc atcatctgca atagtttgcc agtctaatgg acaatccgta actattctat
    13141 tataataaca gtcaccataa aaatctgtgt tctccttccc aatatacaca ttaaaataat
    13201 attcgtctga aaaagacgat ttttgtgcat gaaacatcaa atagtatcca tcttctaaga
    13261 ctcttttcca agtgttcgct ttcttcttaa atcccaacgg tttcaatttt tctgcaattt
    13321 tgtcaatcca cttttttcgc tttattctta caattctaag caatgcttct ttttccagat
    13381 ttttatacct gtcatacatc tccttcacaa gaatacccgt atccctttcc tccgcatgat
    13441 ggaaaatact acttttttgc cgtaaattaa tatcctcatc agcaattccc catcgaacaa
    13501 gagactcttc atctcccttt acgaaagtaa aagccgtaga agcgaatcca taaacaacct
    13561 ccactctgtc attgtatcct gtcaaattca caacaaatgc gctattccct gttgtttctt
    13621 tataaaacat ccagtgcaat gaccgctctt tttgacatat cggttcggaa aaaccattct
    13681 ggagaagaat ccctctgtat aattccattc tatttaccgt ttcttgattt attccatttt
    13741 gctctgtctg cattttttat aagcatccct tctcattaca attattacac attactatca
    13801 taccatttcc gcacctcttc cagaagatat ttaaaaagga aaacccctca tttacaatcc
    13861 ttttcccgct tgaaaataaa atttgtaaat gaagggtttt ctatttttat tcgcccatct
    13921 gcttatactg ctgtgcgata tttttcacat ggtctcccat tctttcaaaa tctgtcagca
    13981 tctctgtaaa aatgattccg ctctgaggtt tacattttcc ttttttcata cgctgcatct
    14041 gtttcttaaa gtacttatct ctcagatcat cgatcttctg ctctgccgca ctggcttcaa
    14101 aaagaacctg cgtcatatcg ctgccctctt catttttcac gatatcaagc gctgtcagac
    14161 actgtttttt catctcttcg atttcttcca atgctacatc agaaaatgac agattccact
    14221 ctttgagatc ttttgcatat cccgccagat tcatggcatg atccccgata cgctccaggt
    14281 tgccgatcac ggcataatat ccattgatga tcttggaatc ctctgtcgat ttctcattgg
    14341 agatcagaga tacgatatac tctgaaatct cacggtttaa ataatcgata taggtctctc
    14401 tttctgaaat cttctccata tctttttcat tatactgaat cagaacatca tatgcttttg
    14461 caatattctt cgataccata tcgcgcattc tctcgatttc atcccgaacc tgtgaaagtg
    14521 ctaccgcact ggagccgatc gcatagttgg attcaaaccg tgtgatgtac ttgagtctgt
    14581 aatctccatc gtcttctttc ttgctgtccg gcaggatctg ggttgcgatt cttgccatga
    14641 cattgccaaa tggaagcagg atcaatgttg ttacaatatt aaatgttgta tgcacattgg
    14701 caatctgtgc caccggattt ccaggtgtga gtgcttccat ccaggagaca aacggagttg
    14761 tcatacagat gaccgtaaac aggatcgatc cgaaaatatt gaacatcaga tggatcacgg
    14821 ttgtacgctt tgcattgact ttcattccaa tcgatgctaa aactgcagta atacaggtac
    14881 cgatgttctg tccaaacaaa atgtatactg cgctggagag cggcaccatt ccagttgccg
    14941 caagcgcctg caggattccg acagatgcag atgatgactg aatgatcgcc gtaaaaatcg
    15001 caccgatcag aatacctacc agcggattct caactgttgt cataaagttg ataaatgtct
    15061 gtgaatcctg aagcggaacc atggcatctc ccatcattgc cattcccata aataaaattc
    15121 caagtccgga aaaaatcccg ctgatatgat gtaccttttc atttttaatg aacatcatcg
    15181 cagctacacc gccgattgca aaaatcggag cgattgccga gatatccagt gcgatcaact
    15241 gacctgtgat cgttgtaccg atatttgcac ccatgattac ccagactgcc tgacttaatg
    15301 tcatcagacc ggaatttaca aatccaacca gcataactgt ggttgcggaa gaagactgaa
    15361 tcacggctgt gatcacagct ccaaccagaa ctcctttaat acggttggaa gtcagcttct
    15421 ccaaaatcga cttcattctg tttccggcag cagcttccag gccgttgctc atcatatgca
    15481 ttccgtacag aaacagtgca agtcccccca gcaaggaaag tatatctgtt atccccattg
    15541 tatctttctc cttttttctt ctctttttgt attttatttt ttcgctcgaa acgagactgc
    15601 cgcaaatggt atcacagcag tctcgttttc tggcaaattt tacaattttg attctagcac
    15661 accgcatttt tccaggtcaa cattctttta cactttttta acaagttctt agcataacct
    15721 taacataagc cttcacagtt cagtctttcc atcaagtatt gaaaagcctc cctgattctg
    15781 ttaaaatcaa agaagattcc gaaaacaacg gaattttaag aaattttaag gagtgaatac
    15841 gatgagtaag attgatgaag taagaagcgc catgatggct gctatgaaag caaaagataa
    15901 agaaagaaag gatgcgcttt ccgcactgct cactgcattg aagaacaagg cgatcgacaa
    15961 acgcgcagac ctgactgaag aagaggaaac acaggtaatc ttaaaagaga ttaaacagct
    16021 caaagaaacc attgagatga cacctgcaga ccgcacggat atcctcgcgg aatgcaacag
    16081 ccgtctcgca gtcctcgaag aatacgctcc aaagatgatg gatgaagcgg aaatcaaagc
    16141 agtggtttca gaagtactta cctctctcgg tctggatgcg ccgaccgcaa aagaaaaagg
    16201 caagatcatg aaagaactga tgccgaaagt caaaggaaaa gccgacggaa agttagtcag
    16261 tgaagtcgtt gcttccttcc tgtcctgatt cactatgaaa gttctgattt tatcctgcaa
    16321 taccggagaa ggacacaatt atgccggcag ggcactcaaa gagtgtatcg aatcgcatca
    16381 tgataccgca gatatgctgg atattatgat gcttgccagt cccagagtat caaaactggt
    16441 ggggaacagc tatgtcaata ttgtccggca tgctccccgc ctttttcagt gtttatacaa
    16501 actgggcgga cttgtcagtt ccgccagaca tcattctccg gtttattatg ccaatgctct
    16561 tctcgccaaa aagctaacgc gctatctgga tacacaccat tacgatgtaa tcgtaacgcc
    16621 ccatcttttt cccgcccaga cactgactta tataaagaaa aagaacctat tgtcccagaa
    16681 agtcgtggct gtagaaacag attatacctg tattccattt tgggaagaga cagactgcga
    16741 ttactacatc atccctcact atgaactgat cgacgaattt acagcaaaag gaattcccag
    16801 agaacgtctg aaaccatacg ggattccggt tcgtccggca ttttctgacc aatctgacag
    16861 acaaaaagcg cgcgtacgct gcgggattcc aacgcatgcc caggtttatc tgatcatgag
    16921 cggcagtatg ggatttggaa agattcagct ctttgttgcc gaactgctcc gtacccgaaa
    16981 gcccggagaa tatgttgttg taatctgcgg aaacaaccgt cgcctgcaaa aaatcctcct
    17041 tgcggaattt ggaaaacaag aaggtgttca gattctggga tacactgaaa aaattgcaga
    17101 ttttatggct gctgccgatg tgcttttcac caaacccggc ggtctgacta ccaccgaagc
    17161 agctgtcaaa gggattccga tcgtacacac acgcccaatt cccggctgtg agacaaaaaa
    17221 tctggctttt tatacagaac ggggattgtc cctgacttcc caaaaactgc acggacagat
    17281 tcttgccggc cgaaaactga tgtcaaacga tgaactgcgg cattccatgt gtacagcgca
    17341 gcacactgtc atcccaccaa atgctgctga gaagacttac cagcttctct gccagctttc
    17401 ttctgcgtat gccgtctcag atgatactgc aaaaaaggag actttatgaa tgagttattc
    17461 tttattttag tcggatatct ttcgggaagt attttatatg cctatctgct tcccaaatat
    17521 atttgccata tcgatatcat gaaagacagt gacgatcaca atcccggtac attcaatgct
    17581 tttgcactgg caggtacaca ggttggtatt cttgtgattg cattggagct tttgaaagga
    17641 tttcttccga tctggctggc ttcacatatt ctggatacaa gaagatggat gtttgcattt
    17701 gttctctgtg cacctgttgc agggcatgcg tttcccctgt tttatccgaa acgtggcgga
    17761 aaagccatcg cagtttcttt tggagtatta ttgggactgc tgccgcgata ccgcccggtg
    17821 ctgcttctca tcttctttta tctgttattt tcttttttga tcgttgtaaa gccgcatctt
    17881 tacagaagca ttcttacgtt tacactgttt tctctgaccg gattgtttta tttccatgat
    17941 gcggtgatca gcatgggcac atttttcatt tcctgcgtgg tcatcatccg acatctggta
    18001 catcatcaga aagagccttt cagcattcgc tttttacagc ggagctgaaa ggctctcttt
    18061 tattttttga tcatagaaat cgcttttgat cgttccagtg tttttgcaag cacataaaag
    18121 attacggaat caatcggaac ggaaactacc tgttttataa atctcgccgg aagaattgcc
    18181 agaaatcctt ttccatacag gatcgtcagc caccaggtac cgagcagaag atttacaagt
    18241 aaagcaaccg taattttggc tgccacgact cttcccaatt gaatcggacg atgatagaaa
    18301 aaaattccgt aaataacagg tcccagcatc gcattcagtg tatacccaaa gaaatacggt
    18361 cccgtcggct tgatcagaaa cttcaggata tccccgattc ctcccatgat cccgccaacc
    18421 accggaccga acagcatcga tgccagctca ttaggaatac cggaaaaacc aatccggata
    18481 aactccgtaa gctgaacaga gaaaaagcct aatatcacac cgatcgcgat caacatggca
    18541 gccactgcca taacttttgt tttcctcaac tcatgccagg agtctacaaa ctgctgtttt
    18601 aattgtttca taaaaaaata cctcctttgc agtaatcctc aagctactca cagaaggtat
    18661 ccttatatca aactggtata tctgtctatg tgcagcagcg ggatgcgaaa tatgtaccgc
    18721 aagcattcac ttttcgtcct gctggcaact ccccgtccaa ccggcactta acgcacatac
    18781 ttctactctg cttttgtttt catttttcgt ggtgattata gcacaatttt caaagacgga
    18841 caactatttt ttctcctgcg gctcttccgg tctcacgatc ggaatctgaa gattgactcc
    18901 gtacggattg atcacaaccc cttttgcctc gtttaccaac atcttcggaa cattttttgc
    18961 ttcaatagcc attgtcttaa actgatcctc tttattgaat tttcgaaact ccaggatatc
    19021 tgtgaaaatc ggctgatatg catctccgtt cttctgtttg agcatcggca ttccgttttc
    19081 tttgtgaacg gctacgataa atctgcctct tccatagttg accagtatct cttcctgcag
    19141 acctttcagc tcttctgtca gttccggctt ttcctgtctg cgcatctcct gcataaaata
    19201 cagagcggtc agatgaagct gcggattctc tacccatact tttccatccg gaaggtcccc
    19261 ggtatttggt cgtatcacca gttctcccaa ctgaagtctt gcttcacttt ccatattttt
    19321 attgatcaaa agcccgttga cgcccatcgg atacaggctt gagaaaaatc caagcagctg
    19381 tttcttttca atctttgcga tctggatcgg aatccgctgt tcaatcagct tctttccctc
    19441 tctctgtgca tcctcctctg tataatataa cagcacttca tcatcaaatg tctcttcatc
    19501 acacaggaca tacggcatct ttgtacacag agacataaag acatacatct cctctgcatt
    19561 tcgcagactg ctcaaaagct gctctttttc cggtaactgt ccgtttttat tttcctcttt
    19621 catgaaactg attcctttct tctgttgata ttccctatct tttaattata ccaaaatttt
    19681 actctgatac caacaagatt ctggattatt ttccactttt cgaactctgg atctcaaaga
    19741 caatccgtgt cagatactcc tcttcttcag acatggtgag gctgtcaaac agaatgtctt
    19801 catagcaatg tgcgcccaac tggattttat tctctttccc atattctaaa attttcttat
    19861 aggtatcctc cagcgtctgc cattttcctt tatgataggc gatcagatac attcccgcgg
    19921 gtctgacact gtagttgact ttttgcggtt tctcatccaa aagctcataa aaaacagaat
    19981 actgatcaaa aataccgctt tgaatctcct tcgtattttg tcggtatcca atggaatatg
    20041 cgcttttgat cccattcctc gcacaatact caaataattc tccgattttt tctgcccata
    20101 cccgttcatt tgtatccgtt gcacaggact ggatcatata tctgtccggt tcttcctcga
    20161 ttctgattgc ttccgtatct gtctgcattg tcttctcaat aatctcactc ttctttacaa
    20221 tccactcttc catctttttc agctgctgca tctgtttcct gattatgctc tgttcttttc
    20281 gaaacaactt cagcagtctc tttggacttc tctgattcat atatcctttg atctcctcta
    20341 aagacacatc cagttctctc aatgtctgga tcacatcgaa cacatccagc tgatcaaaag
    20401 aataaaagcg atagccattc tccagcttga tctccggaga aaacaatcca atcttatcgt
    20461 aatagaacag cgtatgcttt gtcactccaa ccagctttgc aaattctccg gttttaatat
    20521 atctttcctg attcattggt attcccctct tgactataga cttactctat agtttatgat
    20581 aacagggatt gtaagaaaaa acaactgaca ggagctttta ccatgacaaa ttgttatcac
    20641 tacctcaaat aaacatacga aaaataccgc cgcatctcct gcattttcag gttgatgtgg
    20701 cggtattttt tcttttacgc taaaagatca tctttgtcaa tccgattcaa taaaagtgaa
    20761 cacagaactc caagtcccag gcagatcaca tttacgattg ccgcacttaa agatactgta
    20821 aaacttccaa atggtatgat cataaccgtt gccgctacta caattccgac aatggcatgg
    20881 aacacatagg aataaaattt ttccagaaga gcatctatca tcttcgcgaa acatattacc
    20941 gtaacaactg caccgattcc acttgggatc ataatgctca gattcagatg tcccagcccg
    21001 tcaacaaacg gagtatacaa tcccagaggc atcagaaggg tagaaaaact cattcccgga
    21061 acaatcacgc tcaatgccag acacacgccg caaaaaacgt accatcctac attcggcaca
    21121 aggctgactg acagcaggtt caatgttgtc agtatggcaa agatcaccac tgctgctgcc
    21181 accatagaga tccatgatcc gttggtccgt ccttcttttc ccgcctcacg catcagagac
    21241 ggcagcatac ctgcgatcaa tccgacaaaa agacacacgg atggatccgg atacttttcc
    21301 aggaaaaacg acaatatatt ggcaactcca aggaaaccga ctgcgcctcc caaaataacc
    21361 ggaagtaatt tacggatatg ggtaaaaaag tactttcgcg gattggacaa taactccatt
    21421 acaggtctgt aaattccgaa aatgacgcac aggacaccac ctgaaattcc cggaaggaca
    21481 gctccaaggc ctatgagcat cccctgaagc attcgaacta gtataacgaa tattaaataa
    21541 ttgctatatt aaattgatac tgcttcctct tcactgattt catccatttt atact
//
