LOCUS       NZ_JADNBM010000025     13819 bp    DNA     linear   CON 29-FEB-2024
DEFINITION  Sellimonas intestinalis strain 1001217B_150727_G3
            NODE_25_length_27171_cov_62.2066, whole genome shotgun sequence.
ACCESSION   NZ_JADNBM010000025
VERSION     NZ_JADNBM010000025.1
KEYWORDS    WGS; RefSeq.
SOURCE      Sellimonas intestinalis
  ORGANISM  Sellimonas intestinalis
            Bacteria; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae;
            Sellimonas.
REFERENCE   1  (bases 1 to 27171)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Quantification of discrete gut bacterial strains following fecal
            transplantation for recurrent Clostridioides difficile infection
            demonstrates long-term stable engraftment in non-relapsing
            recipients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27171)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, 1470 Madison Ave, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60.75x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/29/2024 02:59:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 60
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 20 of 57
            Pseudo Genes (incomplete)         :: 33 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:35:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 13819
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADNBM010000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13819
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..13819
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13819
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[789:3819](+)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      790..3819
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     gene            184..558
                     /locus_tag="I2A62_RS14140"
     CDS             184..558
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /locus_tag="I2A62_RS14140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /protein_id="WP_195243690.1"
                     /transl_table=11
                     /translation="MENIRFQIEADVLQSLPFKETETSVLFGNLLDNAIEACKKMKTET
                     RWIRVEIRQRKGFLFFTIANSIGKRPQEKDGEFVSDKEDKKVHGYGLKSIRRIVKKYDG
                     SFQCDVVEEEVKISISFSNK"
     gene            580..729
                     /locus_tag="I2A62_RS14145"
     CDS             580..729
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2A62_RS14145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_156169244.1"
                     /transl_table=11
                     /translation="MKENKNLEFAGDLLVEMQEVQKINCSDNVVDRGRTYTLQCTAFLT
                     IFCC"
     CDS_motif       580..609
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MKENKNLEFA"
                     /locus_tag="I2A62_RS14145_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       610..729
                     /prepeptide="core"
                     /locus_tag="I2A62_RS14145_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-2.20"
                     /molecular_weight="4338.0"
                     /monoisotopic_mass="4335.1"
                     /core_sequence="GDLLVEMQEVQKINCSDNVVDRGRTYTLQCTAFLTIFCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKENKNLEFA"
                     /leader_location="[579:609](+)"
                     /alternative_weights="4356.0"
     gene            790..3819
                     /gene="lanM"
                     /locus_tag="I2A62_RS14150"
     CDS             790..3819
                     /GO_process="GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lanM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /locus_tag="I2A62_RS14150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="WP_195243691.1"
                     /sec_met_domain="DUF4135 (E-value: 4.7e-100, bitscore:
                     325.8, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 1.3e-50, bitscore:
                     162.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTYLSERIYQNIDSSKPENVERTQYWNHLLGETTVKELREHNR
                     AFVMYLQDAPFEEKQEVDRGFLHISFQEERGNTDSVRREIGEKKLFFAEFYKTFLKIGK
                     RELESRWSGLGEEVEEMLYEAFEDVLAIRLQNIAFRTLIAELHSYKQKGNLSGETSEQE
                     YESFCKICGSREFFGHISETYPVLIRCLKECTEKTICYYEQVMRWVIEDRESLSCLFGK
                     EKDLGSIVKVESGLSDSHHGGKEVLTIYFANGSQILLKPRSMENEQFYQKLLDWIGERT
                     GTDQYYYPILSGKDHSWCQIVEYKPCNSEAELHQYYQRLGEQLFLAYLLGTNDIHFENL
                     IACGAYPVLIDLEALVHNSRGRSEESIRDTVQSHLWESVLATGILPSYTWNNEGEGIDG
                     SAIHSKKGQKLPFKIPTVAKGETSEMYVTYREGHSSEGKNLAHAKEGMEDLVLYVNDIL
                     EGFSTAYQAVEKERTIFEKELRKGTEIQSRTVLMHTQRYTMLLNSSYHPALLMDGADRE
                     IFLSAIGQGRWEQDEEIVKEEIGSLLRGDIPYFSFAMGEKDLSADGRICVRNLYDKTAC
                     DRIVKRLQGLCGKDRKRQCDFIQTSVELIPENRSGFSNKIYPASKSPAKSQKDMIQENI
                     KELTERLCDYAIWNPRRTEVSWYALRFASSGNYAWEIEPMGVYFYDGWSGLLLLTTMIQ
                     ANQKLKRVEEIQTALKKQVFQYTDNGLVSLNHLQSQNTGAYTGEGSLIYAYLNLYRLTN
                     EKVYLQYAVRHAGIVSQILEEDSCSDLLGGKAGAAWVFLQLYQETQNQRYIQEAERAIC
                     LMLPQMIKTERGVGWNPESVDVPIGGMAHGNAGILMPVLVLWELTKKKEYGWLAEKIWE
                     YEESLYREETGNWMDIRDHSDEGEDTVAWCHGAAGILLSRLWCYEKVNDSKWKKRLKKD
                     IDRAYQKTSAFWKRDSWSLCHGNSGNLWILNLADRMLGKERKERGNYEKIRLLPQERMN
                     PGLMGGYGGVLLHLLVKLIR"
     gene            4001..4321
                     /locus_tag="I2A62_RS14155"
     CDS             4001..4321
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2A62_RS14155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_195243692.1"
                     /transl_table=11
                     /translation="MKKKFRKFFAVVAATVMVLAMGMVSVNASDVQPRGPLCPCGGTLL
                     PSKSYSTWVTYKSVKCVHGYAYGTDQHQKRTVTTTMKCNRCGSGYTTGTTTETRVQCTG
                     HN"
     gene            4512..5753
                     /locus_tag="I2A62_RS14160"
     CDS             4512..5753
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2A62_RS14160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_195243693.1"
                     /transl_table=11
                     /translation="MKNIARRTIALGCVGLLLAGMSGCDKTIEKKDKIVLRVITDPSIQ
                     KEVNSIADFMEKTEEIEVKVEVLPLQESGRDSEIQRLQTQIMAGKGADVYLLDTSPDAA
                     EMKTELFENPYQTMQSGALAPLNEYMKTDSYWNDTTYNKNILKAGQYEGKQYIIPITCY
                     YYVLSGPNNVEKITGDTLKEWLEQADKLDNSELKKAINESLYLTSGRWFQPAVDYEAQQ
                     VLFNKGKWKAFAESYLSHQQERMMDAVNDIENQYQIQPIRSFIGGKGISAKIIPDLEGR
                     RMASVMTYGAVGMSSDHKEKAYEFLMLFLNNKMQEERNENVEQVLNGSAGITVEKKKIS
                     NWLSDRSEETISAVQESFEELDGAYFITDAERTLISNIRETVEWGVEPTKGWEKFLSDL
                     ADKVWEQYEMQVKE"
     misc_feature    complement(5834..5847)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 20.74"
                     /tool="antismash"
     misc_feature    6050..6061
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.02"
                     /tool="antismash"
     gene            6100..7323
                     /locus_tag="I2A62_RS14165"
     CDS             6100..7323
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2A62_RS14165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_195243694.1"
                     /transl_table=11
                     /translation="MRKTVLTLAFFIILSLSLSGCDEKKEEKPVLTILTEREYEDQVKV
                     ARHGFNEKYDDVEVKIQTLSEEPETRETQIQKLRTQIVAGKGYDVYILNNLRYGSGEPL
                     DELPLFDNPYKTMQSGVFSPLNDNINQDSYWKGTSYFDPLLKAGQYEGKQYILPLSFES
                     ELFLSNTEGTGNLNGKTLLEWLEYADSSQDLDLRGTMAQQALHVKGWYQPAADYERKAI
                     LFDEKQWADFFVLMEQFCTEYFSESVDWYGEMTVKGITNEIKRMEIVPDLQGNRIMEVQ
                     AFGAVGMSSDTKQDAYRFLMLFLNRQTEGLEDGIGGRDNPADQEAIKWMLKRSGLSDET
                     IEGTMKILSQFNLAYFSTETESMLQTSVDQAEREKRWEVTKDWEDCKKTGAEIAEKGRR
                     NYETQVKE"
     gene            7818..9041
                     /locus_tag="I2A62_RS14170"
     CDS             7818..9041
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2A62_RS14170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_195243695.1"
                     /transl_table=11
                     /translation="MRNNKGIRVLVICVYLALVMTGCMGEKHTDKKATLTVLTEQAFES
                     TVQMRASAMEDENENLDIQVEVLPLDMTIRENEIQKLQIEIMAGKGPDLYVLTAPMEMS
                     TVEPDLFDNPYQIIQSGAFAPLDSYMDEDSYWKEGSYKKEFLEVGKHDGKQYLLPFTCS
                     YYVLEGIQDIDILEGKSLGEWLEYAKQSENLPLRQCILEGLSQFGGRWFQPAVDYETGQ
                     VRFDKEKWKQFGETYFVTLKKFREQEQYTEKPTYRISHMNEVDLSSEETHFQVMPDLDG
                     RKMAAVSAYGAVGMSSNKKEIGYDFLMGFLYNKTEDGKWTEEAKLQGIPVQENCLNFMR
                     FPDEQIEAVRKGFRELEGAYFITNVEKNMRDALVEENQVGLPEWDSKEFVNRIAEQAWT
                     GFEIQVKE"
     gene            9196..9618
                     /locus_tag="I2A62_RS14175"
     CDS             9196..9618
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2A62_RS14175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_195243696.1"
                     /transl_table=11
                     /translation="MEDKYKHLEMIQNIIQRMATNSFMLKGWAVTLIVAIFALADKDMN
                     QNYFGLTYIPAITFWFLDSYYLQLERKFKMLYDIVRQKAVVEFDLNIQDINYKKTKDKK
                     LRYLNCLFATCEWLFYMPLMIILTFIFQKSILSFLK"
     gene            9642..11924
                     /locus_tag="I2A62_RS14180"
     CDS             9642..11924
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2A62_RS14180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_195243697.1"
                     /transl_table=11
                     /translation="MKSTCYFSYCWDNSTEIMEYLKKEVEKKSGGKVQVIFDKKSFMCS
                     EDFIKREKEIISCDSVVVFFSPEYKKIIDKGDITRGVYREYQLIKSKLKKEEDSILEVL
                     IKGTLKTAVTEDFSSKIAVDLSKGNVIIKAKNGKKVVNTMYRTEMTNLVSTIISKTFSA
                     SRRKDYDFKSKEEMYESLFCDTKSNGKLPKECMFKTNAYTSIMSQRCTFIIGRKGSGKT
                     TFFEILEKYDEGEVKFADKYKILKPISADCIDIYQVYSVLEKYKDDRRLFSRSKMLQLF
                     WNVYIYLCGIYIVCLEEEECNINDDRQPIFHQVGNKLKRIFNINSFEEDNIQETIFTQC
                     VTFFEEFISKYIIDYATPEAFEASMVANFTVQNVFRKFLGKKLFSNFQLAIARCKKRIM
                     IALDGFDTLSEDFRIETNFLLQKENEKDRIEGEKRAEFERLFYRSMVRAVEIMKCSKDP
                     LVQDIHFCIVLPKDRLDQIETADRDISKVNFTDLSWDAIELLEVVVLRLEYIFSIPHRS
                     DMDLWERLSVIMKKHLPAIPEEVGIEINGAVINIPLFEYLLRVSFWRPRDILKYYALLY
                     NANQNMANPEANKIDMQTIKLIINNKADDIIKTEFLYEYDKVFLNISKVLLEFNGKNII
                     LTGKELFDVLSKVSFITTFAFDCEQILNKISLLYELGVLGLKIPAKLMRMRGIGSEWCF
                     SFNEGLKPLELLEYGFSPKNTEIKFVLNPIFSKKLALVFNTNEVVGVFGKEYLVDNHAR
                     KMAIKRF"
     gene            11965..12717
                     /locus_tag="I2A62_RS14185"
     CDS             11965..12717
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426639.1"
                     /locus_tag="I2A62_RS14185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_195243698.1"
                     /transl_table=11
                     /translation="MHVLEVKNLIRNYQKSAQKKSGEDIKVLKGLNFAVDEGEFVGIMG
                     KSGCGKTTLLKVLGMIDKQTDGEVFFMEQNTTDLYGDKLADIRRTQIGFIFQDFYLMDS
                     LTVEENIMLPMILGKENVEVMTKAAAKYAEQFEISHLLKKNPYELSGGEKQRVAICRAL
                     INDPDLILADEPTGNLDSKSGKIVINALNRINREFGKTIVMVTHDPQMASNCSRIILLK
                     DGVILDNLERGEDQEKFYQEIIGRMADL"
     misc_feature    12722..12740
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.96"
                     /tool="antismash"
     CDS             12785..12913
                     /gene="allorf_12785_12913"
                     /locus_tag="allorf_12785_12913"
                     /protein_id="allorf_12785_12913"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MGRIRDSYSYHNLAEFLTEFFLSPVSGASFFCYNNTGKGGKQ"
     CDS_motif       12785..12868
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MGRIRDSYSYHNLAEFLTEFFLSPVSGA"
                     /locus_tag="allorf_12785_12913_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       12869..12913
                     /prepeptide="core"
                     /locus_tag="allorf_12785_12913_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-4.00"
                     /molecular_weight="1514.7"
                     /monoisotopic_mass="1513.7"
                     /core_sequence="SFFCYNNTGKGGKQ"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MGRIRDSYSYHNLAEFLTEFFLSPVSGA"
                     /leader_location="[12784:12868](+)"
                     /alternative_weights="1532.7"
     misc_feature    complement(12926..12939)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: strong, score: 19.68"
                     /tool="antismash"
     misc_feature    13480..13492
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 20.88"
                     /tool="antismash"
ORIGIN
        1 ataatacttc ctcttttctc tgtaagatga ttatatctta ttagccactt ctgttacaac
       61 tttattatag cacttcacta tctggatcag ttactggaaa gctatgtaga agttcaggag
      121 gaaagatgga caagaatccg gaatctggat attctgttaa gtgaaaagaa gaaggaggct
      181 aatttggaga acatccggtt tcagattgaa gcggatgtcc tccaaagtct cccattcaag
      241 gagacagaaa cgagtgttct ctttggaaat cttctggaca atgcgataga agcatgtaaa
      301 aagatgaaga cagaaacaag atggatccgg gtggaaatca gacagaggaa aggatttctg
      361 ttttttacga ttgctaattc gatcgggaaa cgtccgcagg aaaaggatgg ggaatttgtc
      421 tctgataaag aagacaaaaa agttcatggt tatggattga aaagtatccg acgtattgtg
      481 aagaaatacg atggatcctt tcaatgtgat gtagtagaag aagaggtcaa gatctcgatt
      541 tctttttcca ataagtaaag agagaatagg agagaaccta tgaaagaaaa caaaaattta
      601 gaatttgcag gagatttatt ggttgagatg caggaagtgc agaaaataaa ctgttcagat
      661 aatgtagtag acagaggaag aacatataca cttcaatgta cagccttttt aacaatcttt
      721 tgctgctagt caaacaaaac aggatgttct ttggaacatc ctgtttttta aaaccaagga
      781 ggtgtaaaag tgtcggatac ctatttatca gaacgtattt atcaaaatat agattccagt
      841 aaaccagaaa atgtagaacg gacgcagtac tggaatcacc ttctgggaga gacgacggtg
      901 aaggaactcc gggaacataa tagggcgttt gtgatgtatc tacaggatgc tccattcgag
      961 gaaaaacagg aggtggacag aggattcctg cacatttcgt ttcaagaaga aagaggaaat
     1021 acggattccg ttcgcagaga gatcggggag aaaaagctgt tctttgcaga attttataaa
     1081 acttttttaa aaattggaaa acgtgagttg gaaagcagat ggagtgggct gggagaagaa
     1141 gtggaggaaa tgttgtatga agcatttgaa gatgtactgg cgattcggtt acagaacatt
     1201 gcttttcgca cactgattgc tgaattacat tcctacaaac agaaaggaaa cttgagcgga
     1261 gaaacttctg aacaggagta tgagagcttt tgcaagattt gtggaagccg tgagtttttc
     1321 ggccatattt cggagacgta tccggttctt atcagatgtc tgaaagaatg tacagaaaag
     1381 acgatctgtt attatgaaca ggtaatgcga tgggtcattg aagatcgaga gagtctgtcc
     1441 tgtttgtttg ggaaagagaa ggatttgggg agtattgtaa aggtagagtc cggcctgtcc
     1501 gattcccatc acggcgggaa agaggtatta actatctatt ttgcaaacgg aagtcagatc
     1561 ctgctcaaac cacgttctat ggaaaacgaa cagttttatc agaaattact ggattggatc
     1621 ggagagcgaa cagggacaga tcagtactat tatccgattt tgtccggcaa agatcatagt
     1681 tggtgccaga ttgtggaata caagccgtgt aattcggagg cagagttgca ccagtattat
     1741 caaagacttg gcgaacagct ttttctcgcc tatctcttgg gaacaaatga tattcatttt
     1801 gaaaatctga tcgcctgcgg ggcatatcca gttttgatcg atctggaagc cctggttcat
     1861 aattccagag ggcgaagtga ggagtctatt cgggatacag tacaatccca tctctgggag
     1921 tctgtattag ctaccggaat tctcccttcc tacacatgga ataatgaggg agaagggatt
     1981 gatggaagcg ctattcacag caaaaaaggg cagaagcttc cttttaaaat tccaaccgtc
     2041 gcaaaaggag agacctctga aatgtatgtc acttatcggg aaggccattc ctcagaagga
     2101 aaaaacctag cccatgcaaa agagggaatg gaagatctgg tgctgtatgt gaatgatatt
     2161 ttggagggat tttccacagc gtatcaagcc gtggaaaaag aacgaacgat ttttgaaaag
     2221 gagcttcgga aaggaacaga gattcaaagc cgtacagtgt tgatgcatac acagcggtat
     2281 acgatgctgc tgaacagttc ttaccacccg gcgcttttaa tggacggagc ggatcgtgag
     2341 attttcttgt ctgctattgg tcagggacga tgggagcagg acgaggaaat tgtaaaggaa
     2401 gaaatcggtt ctctgcttcg gggagatatc ccatactttt cctttgctat gggagagaag
     2461 gatttgtcgg cggatgggag aatttgcgtg cggaatctgt atgacaaaac tgcctgtgat
     2521 cggatcgtaa agcgattgca aggtttatgt ggcaaggatc gaaagaggca atgcgatttt
     2581 attcagacgt ctgtggaatt gattccggaa aacagaagtg gattttctaa taaaatttat
     2641 cccgcaagca agagtcctgc aaaaagtcaa aaagatatga tccaggaaaa tataaaagag
     2701 ctgactgaac gactttgtga ttatgcgata tggaatcccc ggagaacaga agtgagctgg
     2761 tatgcacttc ggtttgcaag ttccggaaat tatgcatggg aaatagaacc gatgggcgtc
     2821 tatttttacg atggatggag cggactgtta cttttgacga caatgatcca ggcaaatcaa
     2881 aaattaaaac gggttgaaga aatccagaca gcgttgaaga agcaggtctt tcaatacacg
     2941 gacaacgggc tggtatcatt gaatcattta cagtctcaaa ataccggagc atataccggg
     3001 gaaggatctt tgatttatgc ttatctgaat ttatatcgac tgacaaatga gaaagtatac
     3061 ctgcaatatg ccgttagaca cgctggaatt gtgtcgcaaa ttctggaaga agattcctgc
     3121 tcagatttat taggaggaaa agcgggagcg gcatgggtgt ttctgcaact ctatcaagag
     3181 acacaaaacc agaggtatat ccaggaagcg gagcgcgcta tctgtctgat gttgcctcaa
     3241 atgattaaga ctgagcgtgg agttggttgg aatccggaga gtgtcgatgt cccgatcgga
     3301 ggaatggcgc atggaaatgc gggaattctg atgccggtcc ttgttttatg ggaacttaca
     3361 aaaaagaaag agtacggatg gctggcagaa aagatctggg aatacgaaga gtcgttgtat
     3421 cgggaggaga ccggaaactg gatggatatc agggatcata gcgatgaggg ggaagatacc
     3481 gttgcatggt gccacggggc agcggggatt ttgttgtcaa gactctggtg ctacgaaaaa
     3541 gttaacgatt caaagtggaa aaagcgttta aaaaaagata tagacagagc gtatcagaaa
     3601 acgtccgcgt tctggaaacg ggacagttgg agtttgtgtc atgggaacag tggaaatcta
     3661 tggattctga acctggcaga tcggatgttg gggaaggaga gaaaagagag gggaaactat
     3721 gaaaaaatcc ggcttctccc acaggaaagg atgaatccag gactaatggg aggatatggg
     3781 ggagtattac tgcatttgtt agttaaatta ataagataaa atttaatgga ctgaaaaaat
     3841 agaatcatgg tcatgaaatt gacagataat aatgcggaat gtaaaattta actataatat
     3901 taagaacggg gaatgataga tgggaagagg aggagaagcc aatggttttg taaagaaagg
     3961 taggataatg ggaacgagat aaagaaagga gatacaattt atgaaaaaga agtttagaaa
     4021 attttttgct gtagtagcgg ctacagttat ggttttagca atgggaatgg tatcagtaaa
     4081 cgcatctgat gtacagccga gaggacctct ctgtccttgt ggaggaacat tactgccatc
     4141 gaagtcttat tctacatggg taacatataa gtcagtaaaa tgtgtgcatg gatacgcata
     4201 tggcacagat cagcatcaaa aaagaacagt tactactaca atgaagtgta atcgatgtgg
     4261 tagcgggtat acgacgggaa caactacaga aacacgtgta caatgtacag gacataatta
     4321 gtctttgaag ggaatgtact caaagtggaa ttattgggag acagagaata aaaacagaat
     4381 actgttgtta caaataacga atgagtatat ttttagaaga ttctaaacgt tttttattaa
     4441 gccatgtatt ttctttgaaa gcaggaatac atggcttaat taataaaagt aacaagaaga
     4501 ggtgaattat tatgaaaaat atagcgagaa gaactattgc tttaggatgt gtaggactac
     4561 ttttagcagg aatgagcgga tgtgataaga caatagagaa aaaagataaa attgtattaa
     4621 gagtaattac agatccatca attcagaaag aggtgaacag tatagcggat tttatggaga
     4681 aaacagaaga gatagaagta aaagtggagg tacttcctct tcaagagagt gggcgagatt
     4741 ctgagattca gaggcttcag acacaaatta tggcaggaaa aggggcagat gtttatttgc
     4801 tggatacgag tccagatgca gcagagatga aaacggaact atttgaaaat ccatatcaaa
     4861 cgatgcagag tggggcactg gcaccattga atgaatatat gaaaacggat tcctattgga
     4921 atgatacgac atataataag aatattttaa aagcagggca atatgaaggg aaacaatata
     4981 taattccgat tacttgttat tattatgtat tatcggggcc aaataatgtt gagaagataa
     5041 caggagatac tttaaaagaa tggctggaac aggcagataa actggataat tctgaattaa
     5101 aaaaggcaat taatgaaagc ctgtatttaa catcagggcg gtggtttcaa cctgcggtag
     5161 attatgaagc acagcaagtt ttatttaata aagggaaatg gaaggcattt gcggaatctt
     5221 acttatcgca tcagcaggaa agaatgatgg atgcagttaa tgacatagag aaccaatatc
     5281 aaatacagcc gatccgttca tttataggag gaaaaggaat ttctgccaag attattccgg
     5341 atttagaagg aagaagaatg gcatctgtta tgacatatgg ggccgttgga atgtccagtg
     5401 atcacaagga gaaagcatat gagtttttaa tgctgttttt aaataataag atgcaagaag
     5461 aaagaaacga aaatgtagaa caagtcctca acggaagtgc tggaatcaca gtagagaaaa
     5521 agaaaattag taattggttg tccgaccgct ctgaggaaac tatttcggca gtacaagaaa
     5581 gttttgaaga actggatgga gcatatttta taacggatgc cgaaaggaca ttaatatcga
     5641 atatccggga gacagtagaa tggggagtag aaccgacaaa gggatgggaa aagtttttgt
     5701 cggatttggc agataaagta tgggagcagt atgagatgca ggttaaggag tagtgatgag
     5761 gagatcgaac cgaattttat gttttgtagt atgtatactc tttaaatgaa aactaatgac
     5821 ctatcagtgt aaagaaagaa gcgtttcttg ttaaaagtta ggaaagatgc tattctatgg
     5881 atatcttgat cggcgttttt gaatctgttc cggatgaata tttaggagga gaaaaactgg
     5941 atctggttaa tctcttttta ttgtacgggg gttctcctcg atatgagcat cgagttttta
     6001 tattgtcgtt atcgggagac agtgatgcat cctgatatat gagagaggtg aaaaagatta
     6061 cggcttcttg ttgtgtttgg gaatactagg ggggatttta tgagaaaaac cgttcttacg
     6121 cttgcttttt ttattatatt atcattgagc ttatcgggat gtgatgaaaa gaaggaagaa
     6181 aagcctgttc tgacaatttt gacagaaagg gagtatgagg accaggtaaa ggtagcacgt
     6241 catggattta atgagaaata tgatgatgtg gaagtaaaaa ttcagacgct ctctgaggaa
     6301 cctgaaacga gagaaacaca gattcagaag ctaagaacac agattgtagc aggaaaaggg
     6361 tacgacgtat atattttaaa caatttacgc tatggatccg gagagccgtt ggatgaactg
     6421 cctttgtttg acaatcctta taaaacaatg caaagtggcg tcttttcgcc tttaaatgat
     6481 aatataaatc aggatagtta ctggaaagga acaagctact ttgatcctct tttaaaggct
     6541 gggcagtatg agggcaagca gtatattctt ccactttctt ttgagtcaga gctgtttcta
     6601 tcaaatacag aggggacagg aaatctgaat gggaaaacgc ttttagaatg gttggaatac
     6661 gccgacagct cgcaagatct ggatttacgg gggacgatgg cgcagcaagc tttacatgtg
     6721 aaggggtggt atcagccggc ggcagattat gagaggaaag ccatactttt tgacgaaaaa
     6781 cagtgggcgg atttttttgt tttaatggaa caattctgta cagagtattt ttcggaatcg
     6841 gtcgactggt atggtgaaat gacagtaaag ggtattacga atgaaatcaa gagaatggaa
     6901 attgtgccgg atttgcaagg gaaccggata atggaggttc aggccttcgg agccgttggc
     6961 atgtcaagtg acactaaaca ggacgcatac cggtttttga tgctgttttt gaaccgtcag
     7021 acagaaggat tggaagatgg aataggaggg cgtgataatc cggctgacca ggaggccata
     7081 aagtggatgc tgaaaagatc aggcttatct gatgagacga tagaagggac gatgaagata
     7141 ctgtcacaat tcaatctggc ctatttttcc acggaaacag aaagtatgct acagacttct
     7201 gtagatcaag cggagcggga gaaaagatgg gaggtcacca aagactggga agattgtaaa
     7261 aaaacaggtg cagaaattgc ggagaaagga cgcaggaact acgaaacaca ggtgaaagag
     7321 taaaagcagg ggaaaaacgg gtaagcgata tccataatcg tttaactgac agggacaaag
     7381 gaaatagaga tgattttgtt aatgaactca ccaaaatgta aacccccgga tggtagtccg
     7441 agggttttta tttcttcacg gtggaatcac gctgaggtta gtggtaattg ccgtcgctgt
     7501 ctaatcattt gatgataaaa atccaccgcc atcctttcca acagagggag ggttgggatc
     7561 ttattaagta ccccaataac tcaaaaaata atttgtatat aaatgatttt tattatgtag
     7621 atgtaaaatt taaatataga aaagatattg acatacataa atagttagtt aataatagta
     7681 taaatagaac aatataacac ttaattatgt agataaacaa gatttacctg aaaattatta
     7741 gagaattagg aattgatata tggaatataa gtggcagact ggcaccatat ataaaaatac
     7801 taaggaggag acggtaaatg aggaacaata agggaataag agtgttagta atttgtgtat
     7861 atttggcttt ggtaatgact ggatgcatgg gggagaagca cacggataaa aaggcaacat
     7921 tgaccgtact tacggaacag gcatttgaaa gtacagtaca gatgcgggcg agcgcaatgg
     7981 aggatgagaa tgaaaatctg gatattcagg ttgaggtatt gcctctggat atgacaatcc
     8041 gtgaaaatga aattcaaaaa ttgcaaattg aaattatggc tgggaaagga ccggatctct
     8101 atgtgctgac tgctcctatg gagatgtcaa cagtggaacc tgatttgttc gataatccgt
     8161 atcagattat acaaagtggt gcgtttgccc cgttggactc ctatatggat gaggattcct
     8221 attggaaaga gggaagttat aaaaaagaat tcttggaggt tgggaagcat gacggcaagc
     8281 aatatctgct tccctttacc tgctcctatt acgttctgga aggaatacag gatattgata
     8341 ttctagaggg gaaatcgctt ggagaatggc tggagtacgc aaaacaatct gaaaatctgc
     8401 cattgagaca gtgtatctta gagggactgt cacaatttgg aggacgctgg tttcagcctg
     8461 cagtggatta tgagacagga caggtacgat ttgacaagga aaaatggaaa caatttggtg
     8521 aaacatattt tgtgacactg aagaaattcc gggagcaaga acaatatacc gaaaagccga
     8581 cgtaccgaat cagccatatg aacgaagtgg atttgagcag cgaggaaacc cattttcaag
     8641 tgatgccgga tctggatggg cgaaaaatgg cggcggtgtc cgcttatggt gcggttggta
     8701 tgtcaagtaa taagaaagaa atcggctatg actttttgat gggattctta tataataaaa
     8761 cagaagatgg gaaatggaca gaagaggcaa aattacaggg aatcccagta caggagaact
     8821 gtcttaattt catgcggttt ccagatgaac agatagaggc tgtacggaaa ggatttcgtg
     8881 agctggaagg agcgtatttt attacgaatg tggaaaagaa catgcgggat gcgctggtag
     8941 aagaaaatca agtgggactg ccggaatggg attcaaagga atttgtcaat cgtattgctg
     9001 aacaggcatg gacgggcttt gaaatccaag tgaaggaata accgtatagg gtcaggagat
     9061 taaaaggttt taaacccctg atataaattt gtaaaaagtt atatatgtaa attgttgaat
     9121 aagtatatgt aaaatttctt gttataattt gtacacgaat atataatata tgtaaggaaa
     9181 atgggggatt tgtttttgga agataaatat aaacatttgg aaatgataca aaatattata
     9241 caaagaatgg ctactaattc ttttatgctt aaagggtggg cggttacgct tatagtagct
     9301 atttttgctc ttgcagacaa agatatgaat cagaattatt ttgggcttac atatattcca
     9361 gctataactt tttggttttt agattcctat tatttgcagt tagaaagaaa atttaaaatg
     9421 ctttacgata ttgttcgaca gaaggcagta gtggagtttg atttgaatat acaggatata
     9481 aattataaga agacaaaaga taaaaaatta aggtatttaa actgtttatt tgctacttgc
     9541 gagtggttat tttatatgcc gttaatgata atattgacat ttatatttca aaagtcgata
     9601 ttatcatttc tgaaatgata aatagcaggg aggatgaaat tgtgaaaagt acatgctatt
     9661 tttcgtactg ttgggataat tcaacagaaa taatggaata tctaaaaaaa gaagtagaaa
     9721 aaaagtctgg ggggaaagta caagttattt tcgataaaaa aagttttatg tgttctgaag
     9781 attttattaa gagagaaaaa gaaataattt catgtgattc tgtagttgtt ttcttttcac
     9841 cagagtataa gaaaataatt gataaagggg acataacaag aggggtttat cgggaatatc
     9901 aactgatcaa atctaagttg aaaaaagaag aagattcgat tttggaggtt ttgattaaag
     9961 gaactctaaa aacggcagta acagaagatt tcagtagcaa aatagcggta gatctttcta
    10021 aggggaatgt cattataaaa gcaaaaaacg ggaaaaaagt agttaatact atgtatagga
    10081 cagaaatgac taatttagta tcaactatta taagcaaaac attttcagca agtagaagaa
    10141 aagattatga ttttaaatca aaagaagaaa tgtatgaaag tttgttttgt gatacgaaat
    10201 ctaatggtaa attgccaaaa gaatgtatgt tcaaaacaaa tgcatataca agtataatgt
    10261 ctcagagatg tacatttatt atcgggagaa aaggaagtgg aaaaactact ttttttgaaa
    10321 ttcttgagaa gtatgatgaa ggagaagtta aattcgcaga taaatataaa atattaaagc
    10381 ctattagtgc ggattgtatt gatatatatc aagtgtattc agttttagaa aaatataaag
    10441 atgatagaag acttttttca agatctaaaa tgttgcagtt gttttggaat gtatacatct
    10501 atttgtgtgg tatatacata gtctgtctgg aagaagaaga gtgcaatatc aatgatgata
    10561 ggcagcctat ctttcatcaa gtgggaaata aactgaaaag gatttttaat attaatagtt
    10621 ttgaagaaga taatattcag gaaactatat ttacacaatg cgtgactttt tttgaagagt
    10681 tcattagtaa atatatcata gattatgcga caccagaggc atttgaggcc tctatggtag
    10741 ctaattttac tgttcaaaat gtatttagaa aatttttagg aaaaaaactg ttttcaaatt
    10801 ttcaattagc tatagcaaga tgcaaaaaaa ggattatgat tgctttagat ggatttgata
    10861 ctttaagtga ggattttcgt atagagacaa atttcttatt acaaaaagag aatgaaaaag
    10921 atagaataga aggtgaaaaa agggcggaat ttgaaagact attttataga agtatggtta
    10981 gagctgtaga aattatgaaa tgttcaaaag atccattagt acaagatata catttttgta
    11041 ttgttttacc taaagatagg ttggatcaaa ttgaaacggc agatcgggat atttctaaag
    11101 ttaattttac agatttatct tgggacgcaa ttgagttgct ggaggttgta gttctcagat
    11161 tagaatatat ttttagtatt ccccatagat cagatatgga cttatgggaa aggctttcag
    11221 ttataatgaa gaaacatcta ccggcgatac ctgaggaggt aggtatagag atcaatggag
    11281 cagtgataaa tattcccctg tttgaatatc tgttaagggt atcattttgg agaccaaggg
    11341 atattctgaa atattatgct ttattataca atgctaatca gaatatggct aatccagaag
    11401 cgaataagat tgatatgcaa acaatcaagc tgattataaa caataaggct gatgatatta
    11461 ttaaaacaga atttctatat gagtatgata aggtgttcct aaatattagt aaagttttac
    11521 ttgaatttaa tggaaaaaat attattttaa caggaaaaga gttatttgat gtgttgtcaa
    11581 aggtatcatt tattacgacg tttgcatttg actgtgaaca gatacttaac aagattagct
    11641 tattgtatga actaggggtg ttgggactga aaattcctgc aaaattgatg agaatgagag
    11701 gcataggaag tgaatggtgc ttttcgttta atgagggctt aaagcctttg gaattattag
    11761 aatatggatt ttcgccaaaa aatactgaga ttaaatttgt attaaaccct atttttagta
    11821 aaaaattggc attggtattt aatactaatg aggttgtagg cgtatttggc aaggaatatc
    11881 ttgtagataa tcatgcaagg aaaatggcaa ttaaaagatt ttagtaaatt gtcattggcg
    11941 tttatttagg aggaaaaaca gatcatgcat gtattagaag taaaaaattt aattcggaat
    12001 tatcagaaat cagcacagaa aaagagtggg gaggatatca aggttctgaa agggctgaat
    12061 tttgctgtag atgaaggaga atttgtcggc atcatgggga aatccggttg tgggaagacg
    12121 accctgctga aagttctggg tatgatcgac aagcaaacag acggagaggt tttctttatg
    12181 gaacagaata cgacagatct ttatggggat aagctggcgg atatccgaag gacgcagatc
    12241 ggattcattt ttcaggactt ctatctaatg gatagtctga cagtagaaga aaatatcatg
    12301 ctgccgatga tcctggggaa agagaacgtg gaagtgatga caaaggctgc cgcaaagtac
    12361 gcggagcagt ttgagatctc ccatctgctg aagaaaaatc catacgaact gtccggtggg
    12421 gagaagcagc gggtggccat ctgtcgtgcg ctgatcaatg atccagacct gatactggcg
    12481 gatgagccga ccggaaatct ggactctaaa tcagggaaaa tcgtaatcaa tgcgttgaac
    12541 cggatcaacc gggagtttgg caagaccatc gtcatggtaa cacatgatcc gcagatggca
    12601 agtaattgta gccggatcat cctcttaaag gacggagtga tcctggataa tttggagcgc
    12661 ggagaggatc aggaaaagtt ttatcaggaa atcatcggcc ggatggcgga tttataatat
    12721 cagaaattat acagtatacg acgggagatg gtgtacgtgc tgaacagcat ctcccgtcct
    12781 ttctgtgggg cggattagag attcgtactc atatcataat cttgctgaat tccttacaga
    12841 attctttttg tctcctgttt ccggtgcttc ttttttctgc tataataata ccggaaaagg
    12901 aggaaaacaa tgagttttgt acatttgcat gttcatacag aatatagttt gctggatggt
    12961 tccaataaga tcaaagagta cgtggcaagg gtcaaagagc ttggcatgga cagtgccgcc
    13021 attacagacc acggcgtcat gtacggagtg atcgattttt ataaagaagc aaaaaaggca
    13081 gggatcaatc cgatcctggg gtgcgaagtt tatgtggcgc cgggatcccg gtttgaccgg
    13141 gagaccggac acagtgaaga tcgttactat caccttgttt tgttggccga aaatgaagag
    13201 ggctaccaca atctgatgaa gattgtgtcc aggggattta ccgaggggta ctattataag
    13261 ccacgtgtgg acaaggagat cctcagacag tacagcggcg ggctgattgc gctgagtgcc
    13321 tgtcttgccg gggaagtaca gaagaatctg gtacggggac tctacgaaga ggcaaagcag
    13381 gcggcctacg aataccagga catttttggg aaagggaatt tcttccttga attgcaggat
    13441 cacggtataa gtgagcagca gacggcgaat caggggcttt tgcgtcttgc aaaggagacg
    13501 gggattgagc tggtggcgac caacgactgc cactatacgt atgcggagga tgaaaagccg
    13561 cacgatattc tgctctgcct tcagacaggg aagaagctct ccgatgagaa ccggatgcgt
    13621 tatgagggcg ggcagtacta tgtcaagagc gaagatgaga tgcgggggct gtttccgtat
    13681 gctttacagg cgcttgagaa tactcagaag attgctgatc gatgccatgt ggaaatcgag
    13741 ttcggtgtga caaagcttcc gagatacgat gtgccggacg gctatacatc ctgggaatat
    13801 ttgcagaagc tttgctttg
//
