LOCUS       NZ_JADNBM010000010     21041 bp    DNA     linear   CON 29-FEB-2024
DEFINITION  Sellimonas intestinalis strain 1001217B_150727_G3
            NODE_10_length_130588_cov_61.8764, whole genome shotgun sequence.
ACCESSION   NZ_JADNBM010000010
VERSION     NZ_JADNBM010000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Sellimonas intestinalis
  ORGANISM  Sellimonas intestinalis
            Bacteria; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae;
            Sellimonas.
REFERENCE   1  (bases 1 to 130588)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Quantification of discrete gut bacterial strains following fecal
            transplantation for recurrent Clostridioides difficile infection
            demonstrates long-term stable engraftment in non-relapsing
            recipients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 130588)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, 1470 Madison Ave, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60.75x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/29/2024 02:59:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,958
            CDSs (with protein)               :: 2,958
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 4 (16S)
            tRNAs                             :: 60
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 20 of 57
            Pseudo Genes (incomplete)         :: 33 of 57
            Pseudo Genes (internal stop)      :: 13 of 57
            Pseudo Genes (multiple problems)  :: 7 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:35:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4992
            Orig. end                         :: 26033
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADNBM010000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21041
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="nucleoside"
                     /region_number="1"
                     /rules="(LipM or LipV or LipU or TunD or pur6 or pur10 or
                     nikJ or nikO or truD)"
                     /tool="antismash"
     cand_cluster    1..21041
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(LipM or LipV or LipU or TunD or pur6 or
                     pur10 or nikJ or nikO or truD)"
                     /kind="single"
                     /product="nucleoside"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21041
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[10000:11041](+)"
                     /cutoff="20000"
                     /detection_rule="(LipM or LipV or LipU or TunD or pur6 or
                     pur10 or nikJ or nikO or truD)"
                     /neighbourhood="10000"
                     /product="nucleoside"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11041
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(LipM or LipV or LipU or TunD or pur6 or
                     pur10 or nikJ or nikO or truD)"
                     /neighbourhood="10000"
                     /product="nucleoside"
                     /protocluster_number="1"
     misc_feature    157..171
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.98"
                     /tool="antismash"
     gene            816..1568
                     /locus_tag="I2A62_RS08785"
     CDS             816..1568
                     /EC_number="2.1.1.223"
                     /GO_function="GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374154.1"
                     /locus_tag="I2A62_RS08785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="WP_053768720.1"
                     /sec_met_domain="PCMT (E-value: 9.7e-08, bitscore: 24.9,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCKLKDGERLDDLQTHGYEIIQHPGKFCFGMDAVLLASYAKVKRG
                     ERALDIGTGTGIIPILMTVKTDGEEFTGLEIQEESADMARRSVVHNHLEDKITIKTGDI
                     KEAADYFPAASFDVITTNPPYMIGQHGIQNVNSAKTIARHEVLCTLDDILRESARLLTF
                     HGRFYMVHRPFRLAEIFTKMTSYGIEPKRIRFVHPYVDKEPNMVLIEGSNKGRSRMQVD
                     PPLIVYEKDGSYTGELLTNYGMGRAGEK"
     gene            1579..2436
                     /gene="rsmI"
                     /locus_tag="I2A62_RS08790"
     CDS             1579..2436
                     /EC_number="2.1.1.198"
                     /GO_function="GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rsmI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TP_methylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245931.1"
                     /locus_tag="I2A62_RS08790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="16S rRNA (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="WP_053768721.1"
                     /sec_met_domain="TP_methylase (E-value: 2.2e-31, bitscore:
                     102.3, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGTLYLCATPIGNLEDMTFRVIRILKEVDLIAAEDTRNSIKLLN
                     HFEIRTPMTSYHEYNKIEKGRKLVEKLQEGKDVALITDAGTPGISDPGEELVRMCKEAG
                     IAVTSLPGACACITALTLSGMSTRRFAFEAFLPQDKKERRRILDEVCTETRTMIFYEAP
                     HRLVKTLRELENTLGSHRRITVCRELTKKYETVYSDSLGGAVRYYEEVPPKGECVLVLE
                     GKSQEEVEEEQKKGWMPLSVEEHLQIYLNQGMDKKEAMKRTAKDRGVSKRDIYQEMIGK
                     KGEI"
     gene            2568..3680
                     /gene="ugpC"
                     /locus_tag="I2A62_RS08795"
     CDS             2568..3680
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ugpC"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527933.1"
                     /locus_tag="I2A62_RS08795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="WP_024733965.1"
                     /transl_table=11
                     /translation="MASLSLKHINKVYPNGFEAVKDFNLEIADKEFIIFVGPSGCGKST
                     TLRMVAGLEEITSGELKIGDKVVNDVEPKDRDIAMVFQNYALYPHMTVYDNMAFGLKLR
                     KVPKPEIDKMVREAAKILDLEKLLDRKPKALSGGQRQRVAMGRAIVRNPKVFLMDEPLS
                     NLDAKLRGQMRIEISKLHQRLGTTIIYVTHDQTEAMTLGTRIVVMNAGVVQQVDTPRKL
                     YDEPCNLFVAGFIGSPQMNFLDAKCEVNGDKVLLNVGPAKIELPPAKAKKLIDGGYAGK
                     TVVLGIRPEDVHDEPMFIASSPNTVIEATIRVYEMLGAEVYLHFDYEGATMTARVDPRT
                     TARNGDTVKFAFDAEKIHVFDKETEMTITN"
     misc_feature    3796..3809
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.01"
                     /tool="antismash"
     misc_feature    complement(3988..4003)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.59"
                     /tool="antismash"
     gene            4031..6637
                     /locus_tag="I2A62_RS08800"
     CDS             4031..6637
                     /EC_number="7.2.2.10"
                     /GO_function="GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844948.1"
                     /locus_tag="I2A62_RS08800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="WP_048621198.1"
                     /transl_table=11
                     /translation="MKQYYQMGSDETRMQINGSLESLSSEQVREHQKKYGPNELQEEKK
                     KSIGMIFLEQYKDFLVIILIISAIISGFLGDAESAAVILIVITINAILGTVQTVKAEKS
                     LNSLKKLSGPEAKVERDGTVMQIPSREVTVGDIVILEAGDQVPADGRIIEQASLKVDES
                     ALTGESIPVEKSIDLIEGEVPLGDRVNMVFSGSFVTYGRGKFVVTGIGMETEVGKIASL
                     LKTTSEKRTPLQVNLDQFGQKLSILILILCAILMGINVWRGESFGDAFMFAVALAVAAI
                     PEALSSIVTIVLSFGTQKMAREHAIIRKLQAVEGLGSVSVICSDKTGTLTQNKMTVEDY
                     YIAGHRMSKEEVSTEQDEVRRLMLASILCNDAKNVNGQEIGDPTETALVTFAGNCGMDV
                     EEIRQKYGRISEVPFDSDRKLMSTVHETSEGVIMFVKGAVDVLSGRMCKVRRNGEEEPI
                     TDEDRKHIASVNQDFSRQGLRVLGFAYKRLETKTEIVEDDEDDLTFLGLISMMDPPREE
                     SKAAVAECIRAGIKPVMITGDHKVTAAAIAKRIGILKDESEACEGADIETMSDAELKDF
                     VEHISVYARVSPEHKIRIVKAWQEKGNIVSMTGDGVNDAPALKQADVGVAMGITGTEVA
                     KDAASMVLTDDNFATIVKAVENGRNVYRNIKSSIQFLLSGNFGAILAVLYASIAGLPVP
                     FAPVHLLFINLLTDSLPAIALGLEPHRKDVMDEKPRQKDESILTRDFLAKIVTEGLSIG
                     VMTMIAFMIGYTSEGALLGSTMAFGTLCTSRLFHGYNCKADRPVLFKKGFFNNIYLQGA
                     FLIGFVLITGVLTIPALHGIFSVTTLNITQLFTVYGLAILNIPVIQCLKAIRFKLR"
     gene            6650..7822
                     /locus_tag="I2A62_RS08805"
     CDS             6650..7822
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266696.1"
                     /locus_tag="I2A62_RS08805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AI-2E family transporter"
                     /protein_id="WP_024733966.1"
                     /transl_table=11
                     /translation="MELNNENMKKIRHLIVFTALIILCFWKYTIVFQAVKFVIGVVFPF
                     ILGGAIAFVFNVPMHFIENRLIQEKWKEKYKIVRKTARPVSLVLTIIFVIGIVGGVLFG
                     VLPQLTGTIAKLGYSIQTFIPKVQEWANDWFHNNKEVMAYVNQLEFNWDKVIEVAVDWV
                     SNGAGNIVESGVTAAVNIVSGFATFFIAFVFACYILLQKEKLGVQAKKVLFAFVQKGRA
                     EAAIEVCSLTYRTFSNFLTGQCMEAVILGTMFVIAMSIFKMPYAWLIGILIAFTALIPI
                     FGAFIGCAVGTFLIFMIHPIQALWFIVLFLVLQQIEGNLIYPHVVGNSVGLPSIWVLAA
                     VSIGGSLMGVVGMLIFIPIVSVLYALFREIVYLKLKKNNIKIKDILQSEK"
     gene            7875..8462
                     /locus_tag="I2A62_RS08810"
     CDS             7875..8462
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844795.1"
                     /locus_tag="I2A62_RS08810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleoside recognition domain-containing protein"
                     /protein_id="WP_117493729.1"
                     /transl_table=11
                     /translation="MLNSLWAGMILIGIIFAAFTGRIPEITDAALDSSKEAVTLCITMI
                     GVMAFWMGLMEIASKAGMIEKGAKMLKPFVRFLFPEVPEGHKAGEHITTNIIANFLGLG
                     WAATPAGLKAMEELGKLNHGSPVASNAMCNFLILNISSLQLIPVNVIAFRSQYGSVNPT
                     AIVGAGIVATAISTGTAIVFCKIMDRKKTRKS"
     gene            9243..9518
                     /locus_tag="I2A62_RS08815"
     CDS             9243..9518
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2A62_RS08815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_195243255.1"
                     /transl_table=11
                     /translation="MEIKMKKIVINVAKKSVGKSIPFSMFKVKKPDNFEEIIKRVEEER
                     IKQRVKKFFTKSKQFKNNIISFMKMLFWSIFLENILRHIYLVINKS"
     gene            9807..9998
                     /locus_tag="I2A62_RS08820"
     CDS             9807..9998
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2A62_RS08820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_117493731.1"
                     /transl_table=11
                     /translation="MEEAVLKIIRKYFSFSISVEYEEEWITANQIKEKLRKIKENTGNE
                     KPILKVEESTIKMKGDDK"
     gene            10001..11041
                     /gene="truD"
                     /locus_tag="I2A62_RS08825"
     CDS             10001..11041
                     /EC_number="5.4.99.27"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /GO_process="GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /gene="truD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     nucleoside: truD"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /locus_tag="I2A62_RS08825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="WP_117493732.1"
                     /sec_met_domain="truD (E-value: 2.1e-45, bitscore: 147.5,
                     seeds: 30, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRYKIKTNPEEFLVSEISNIQTTLSQDKYKIFLLEKKGYSTFDAI
                     ASLSSYLSIDQAEVGYAGLKDEDGITKQKISIPSNLINEKHVEAFNRKFIDRDKYMHIT
                     YIGFTRKKIAINNLLGNSFKIRIRNMEKREVDYLLKVRKINLNYVNYYGIQRFGLPDKE
                     KKTHLIGKFLLQGDYEEAFKLACTVEDNFDKSINSSYYFEKMDFRKRSFYYNAYSSYVF
                     NESLSNLVKEYSQNVLNEEENISFYFPINENGILNIWAHKKMLPIKRYYYDESNNLTEK
                     RSSRFCISNSVVKIIQVQEDEIYHKWSADIEFMLPSGNYATVAIMQLENIMSNLLEKSH
                     EKFIDF"
     gene            11019..11813
                     /locus_tag="I2A62_RS08830"
     CDS             11019..11813
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /locus_tag="I2A62_RS08830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAD-IIB family hydrolase"
                     /protein_id="WP_173022082.1"
                     /transl_table=11
                     /translation="MKNLLISDFDGTLYHNNINIYKENLLFIKRWASSPDNYFSIATGR
                     LAREIYNICTKENILYEFIVCNEGSSIIYNKFDKNDIFTFTASSCLQIYRSLIKLVESK
                     QLKYFLETDGEYGFIDCYNLDDKCRNIVREKKKTKYNYLTFKDKNSDIKTSIFILKILD
                     ISYVDLVKKILEKELGNICSISQAYTYMALIPKKASKVEGIAKALKDNKRKFDRILMVG
                     DSRNDIESLRKYMGFTIKGSEAEKILPELPSVQYLNEIENYL"
     gene            11847..12653
                     /locus_tag="I2A62_RS08835"
     CDS             11847..12653
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /locus_tag="I2A62_RS08835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC-2 family transporter protein"
                     /protein_id="WP_117493734.1"
                     /transl_table=11
                     /translation="MEKYLAFSKLTLSSKAIYRKAVLFDIFGIALSILLYFFLWSKVYA
                     GHDTIADFSYYEMVFYIIISQTLASQFYGSSGINNTLSEWIYEGNISIEILRPVSLIAN
                     LFARKLGDVKFFLMTKAGPIIIIVMFGFYGLRKVDMPGIINISFSVLCLVISLIIVFFF
                     ECMVGFISFFTMNSHGISIVKNALMLLMSGSAIPYFILGNRIGLWLNLLPFSGTISIPI
                     QILLGKYSVILSLRYICVQLLWSLVFGVITLLSYQKVIKRVVVQGG"
     gene            12657..13457
                     /locus_tag="I2A62_RS08840"
     CDS             12657..13457
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /locus_tag="I2A62_RS08840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC-2 family transporter protein"
                     /protein_id="WP_117493735.1"
                     /transl_table=11
                     /translation="MLKRKGFFYLSLGKAYIRIGFQILKQYPLDMILSSLSMLLRDSVN
                     FAAIITAISIMGDMGKWKVYEIVFLYSFSMMIEASSQIFFDAIWYLGSMIRKGKLDIIL
                     IRPAGSLFQFFGSKIQLQASLSFLVSFFVFVLSIAKMDYIITGKSIICFIFFLLFCTLL
                     NSSILGIFNCANFWLINAGEISSIVYKLRDFSKYPLFLFPVWVINIITYVIPFATTSYY
                     PTTFLLFGENNNIIGLIIGETIFFFSVFVLIWRRGIGTYSSTGT"
     gene            13495..14469
                     /locus_tag="I2A62_RS08845"
     CDS             13495..14469
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976331.1"
                     /locus_tag="I2A62_RS08845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_117493769.1"
                     /transl_table=11
                     /translation="MVEVKDLYKEYSIKKRGATHYFLKRSERTIIKAVDHVSFIVNQGE
                     AVGYVGPNGSGKSTTIKMLTGVLQPTSGEIRVNGIDPSKNRIENNRNIGVVFGNRSQLW
                     WDVPIIESFGFLQKLYQIPDSIYKNNLEYFVDKLELQNIINRPERQLSFGQRIRCNIVA
                     AFLHNPAVVYLDEPTIGLDLESKERMRNIINKINKERETTIFITSHDLLDIELLCNRLL
                     IIDNGKIITDTDFDNMLKIYNRKQIFKCRVIDKLIDIPMNLKKDIKVRRLEGNHLEVEF
                     DNQKIDIKELLEELSRYVSFSDVSIESMSIEDIVKKILIRKEQ"
     gene            14472..15728
                     /locus_tag="I2A62_RS08850"
     CDS             14472..15728
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /locus_tag="I2A62_RS08850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_117493736.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 9.7e-08,
                     bitscore: 25.0, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHFIVLMMSIFLLIGIIRGILRIKDTKTSLKNYTDKLKGNIVREF
                     SDIDIIIPVYNEQKIIKQSINNFLPLIEKGVRVTYVTTQKEESSPTTKELLRKYIAEYH
                     LVGKCMIVDYPSKKGVMSHQLNFAIKQIKENRIICIYNVDSRIDIRTIEYILENKDILS
                     EGVFQQYSFFSHKKENGVLINSAIAWQNRWTIGYEYPKALKEKNGKKWYNFNYVIGHGL
                     IFTRRMYDKFGGFSEEEINEDNVLGYKLHLKNIRITPVPIFENADFASSLGIYLKQQST
                     WFNGPAYAFKYAYKYYQNEKNDLGNITLVSISNFKNALNWALLPIGLDICLIYLVVNSQ
                     ILTFILFLLLVVIYATVLDFLSEKVLVSHGYLKKQNKASYFFDTCFLLGIHFVGPLITI
                     TKILFRKNSISNKYKTEKS"
     gene            15765..16757
                     /locus_tag="I2A62_RS08855"
     CDS             15765..16757
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /locus_tag="I2A62_RS08855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_117493737.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.4e-22, bitscore:
                     73.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNKKKSILFFNARRRVNMAKKMIEYLKNRYTEYQLISSDTDYLD
                     PISYVTDDFVVLPSIESQEFESVFLEVIKKKNIVGIIMWNDKDFAAIEKMRLKIEKLGC
                     KILMPKEKKYEICNNKMLTDKFLRTHALYTPQIYENPDKIKKYPVIIKPKNGAGCVDVY
                     KADNLEEAIVFYKKVPNPIIQEYIEGVHYTVDTYFDRCDGVLCSVPRKRIKVREAEVMI
                     AKIEMQEEVMNVAQQLTKFFSEEGPMNIQVILSVDDGKPYIIDIHCRIGGGSELSIEGG
                     VPLHEWIIDDIIGGEKRIQYIVNDGLIMTRYFDAFFINENSSIKNNIKR"
     gene            16770..17573
                     /locus_tag="I2A62_RS08860"
     CDS             16770..17573
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2A62_RS08860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_117493738.1"
                     /transl_table=11
                     /translation="MDKSKLILKVKSYLNNLPEEIFDKNYLTTASVFLSGSTGWGIKEG
                     FDEIADWDLHIILDNISYEHYKKKYGEHHVIDDQQHQPKVFAQIRSISWLDIRLQNIQN
                     ADCTYLWIYNNALLIQDNLHIEEKIDEWNSFFKNNIEEILKYHFVTFAVRRLDAVSAIK
                     RNTVIGTNISKIEMIKEALIIISLLHQSPYPYNKWMEKYVLQLGEDGEKCVELCKRCIF
                     EINFESNARVLKNFLVAEIKKQVGNLPWLDEWWKYNENPPVVYIR"
     gene            17586..18116
                     /locus_tag="I2A62_RS08865"
     CDS             17586..18116
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2A62_RS08865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_117493739.1"
                     /transl_table=11
                     /translation="MGYWASFIALDLEKIEANKIVREMKDKLKKYNNIISWHNPNYLHL
                     TLNYFDWLEETQIGIIEKLLTEVQNKIPDKILIKDEIICIGGNENKYVALEIDKKNLIP
                     LKKDMDTLLIKHRIDYKIQEFRPHISLGRIKKGSLIKLKNIEQAIGVKGISLYESKEYS
                     ISIMSRCKQYNKK"
     gene            18146..18883
                     /locus_tag="I2A62_RS08870"
     CDS             18146..18883
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /locus_tag="I2A62_RS08870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_117493740.1"
                     /transl_table=11
                     /translation="MGKIVVTTGGNGTLGKSVVKKLIKEGYFVISLVHTLSDEKKVNIL
                     EVETDLSDINAIQRSIERVKLIISKIEIESLSIIHMAGIYEKQKFPVKIGDMQKWNEIF
                     WVNTFSFYFIVSELVDLFKLIRRGNIIAVSSNLTKLVNNDTAAYIGAKAALEKITVQLA
                     YQLGEYNICCNIICPGFFFSAMSNNIPLKVLEEIKKNTPLNQVVTEETIATVILDILCS
                     KNLGMTGQIITVDGGNTIGFAKN"
     gene            18849..19823
                     /locus_tag="I2A62_RS08875"
     CDS             18849..19823
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SPASM"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /locus_tag="I2A62_RS08875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="WP_117493741.1"
                     /sec_met_domain="PF04055 (E-value: 2.7e-15, bitscore: 50.0,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 2.6e-12, bitscore: 40.0,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEGIQLGLQRIKFPKIIEFQTQTLCNGRCTICPYEQVKKIIPYFR
                     MSDEKVNLLIKELAEHKEDIERVVPYLNNEPFLDKRCISILRRLKKNHFIELSTNASLL
                     SKEVAETIVNEKLVDDFRISFFGGTKESYSRLMRGLQFEKVVENIQYFLAINRRNINTQ
                     ITMILLPWLDMKKSIEEVKNLFPNENIVTFGYLDRAGNNKIFRNNLCMYEKDKIKLCGC
                     NLERPFERMCIMSNGNVILCSQDWKNEIVQGNIFETSISEVWNSNESINIKKMVKGEVE
                     SSDNFLCKRCKLAKIKVEDRMVLNFAGDKYMDEWDRKRYEYKD"
ORIGIN
        1 ggtgattgtc gaaacggcgc gggggattga gtttggacat gtagtgatgg gtccgaaaga
       61 agtggaggaa gaccagatca cgcagccgtt aaagtcagtg atccgccttg cgaataacga
      121 ggacagaaag atcgaagaac gtaaccgtga aaaagagaaa gaagcgttcc aaatctgcct
      181 ggagaagatt cgcaaacata agctggagat gaaattgatt gatgcggaat atacatttga
      241 caataataaa gtactgtttt attttacggc agacggaaga attgactttc gggagcttgt
      301 aaaagatctg gcagcggtgt tcaggacaag gattgagctc aggcagatcg gagttcgtga
      361 tgaaacgaaa ctgcgggggg gcatcggtat ctgcggaagg gagctctgtt gccacaccta
      421 tctttcggag tttgcgccag tttccatcaa gatggcgaaa gagcagaatc tgtctctaaa
      481 tccgaccaag atttccggtg tgtgtggaag actgatgtgt tgtctgacaa atgagcagga
      541 gacatatgag aagcttaaca gcaggctgcc gtccacaggc gatacggtga caacaccaga
      601 aggactgcgg ggagaagtgc agagtttaag tgtattgagg cagctagtca aggtagttgt
      661 gacactggaa aacgacgaga aagagatacg cgagtataag gcttctgagc tgaaattcaa
      721 gtcacgcaga aagaaaaatg atatgcgcct ttccaaagag gaaatgaaag agcttgtagc
      781 acttgagaaa aatgaaggag cgtcgaaatt ggacaatgtg taagttaaaa gatggggagc
      841 gcctggatga tctgcagact catgggtatg agattattca gcatccggga aagttctgtt
      901 ttggaatgga tgctgtgctt ttagccagct acgcaaaggt caaaaggggt gagcgtgctc
      961 tggacatcgg caccgggaca gggatcattc ccattttgat gacagtcaag acggacggag
     1021 aggaatttac aggtttggaa atccaggagg aaagcgcgga tatggccaga agaagtgtcg
     1081 ttcacaacca tctggaagac aaaattacga taaagacagg tgatattaaa gaagcggcag
     1141 actactttcc ggctgcttct tttgacgtca tcacaacaaa tccaccctat atgatcgggc
     1201 agcatgggat tcagaatgtc aacagtgcca agacgatcgc cagacatgag gtactctgta
     1261 ctttggatga tattttgcga gagagcgcaa gacttctgac attccatggg agattctata
     1321 tggtacatag accgtttcgt ctggcggaga tttttacaaa gatgacaagt tatgggattg
     1381 agccgaagag aatccgcttt gtccatcctt atgtggataa ggagcccaat atggttctga
     1441 tcgaggggag taataagggc agatcacgga tgcaggtgga tcctccactg attgtctatg
     1501 aaaaggatgg aagttatacc ggggagcttc tcaccaacta tgggatggga agggcaggag
     1561 aaaagtaaag gaggaactat ggcaggaaca ttatatttgt gtgcgactcc gatagggaat
     1621 ctggaagata tgacgttccg tgtgatccgg atcttaaaag aagtggatct gattgcggcg
     1681 gaagatacga gaaacagtat taaattgttg aatcactttg agatccgtac acccatgaca
     1741 agttaccatg aatacaataa aattgaaaaa gggagaaaat tggtggaaaa attgcaggag
     1801 ggcaaggatg ttgcgctcat aacagatgcc ggcactccag ggatatcaga tccgggagaa
     1861 gagctggtac gcatgtgtaa ggaagcaggc atcgctgtca catcgcttcc gggggcgtgt
     1921 gcctgcatta cggcgctgac tttgtccgga atgtcgacca gacgatttgc ttttgaggca
     1981 tttttgccac aagataagaa ggaacgccgc cgtatactgg atgaggtgtg tacggagact
     2041 aggacaatga ttttctatga agcgccgcac agactggtaa agacattaag agaactggag
     2101 aacactcttg gcagccaccg gagaatcacg gtgtgccggg agcttacaaa gaagtacgag
     2161 actgtataca gcgatagtct tggaggagcc gtcaggtatt atgaggaagt gccgccaaaa
     2221 ggagaatgtg tactcgtcct ggagggaaag agtcaggagg aagtggagga ggaacaaaag
     2281 aaagggtgga tgccactttc tgtggaagag catctgcaga tttatttaaa ccagggaatg
     2341 gataaaaagg aggctatgaa acggaccgca aaagacagag gggtaagtaa gcgggatatc
     2401 taccaggaaa tgatcgggaa aaaaggtgaa atatgaccca aaagagttcg gcaccttgta
     2461 cagaatcccc aaaaatgttt gtgcaatcat gcactagcgt ttttgaccat ttttttttat
     2521 actaattaca tcgaataaaa acggttaaaa tttaggagga agcaccgatg gcaagtttat
     2581 cattgaaaca tattaataaa gtttatccaa acggtttcga ggctgttaaa gatttcaacc
     2641 ttgaaattgc tgataaagaa tttatcattt tcgtaggacc ttcaggatgc ggaaaatcta
     2701 caacccttcg tatggttgcc ggactggaag agatcacatc cggagaactg aaaattggcg
     2761 acaaagtagt aaacgatgtt gagccgaaag acagagatat tgcgatggta ttccagaact
     2821 acgctctgta tccacatatg acagtttatg acaacatggc attcggactg aaattaagaa
     2881 aagttccgaa accggagatt gacaagatgg ttcgtgaagc agcaaagatc cttgatcttg
     2941 agaaactgct tgaccgtaag ccgaaagctc tttctggtgg acagagacag cgtgttgcca
     3001 tgggacgtgc gatcgttcgt aatccgaagg tattccttat ggacgagcct ctttccaact
     3061 tggatgcgaa attaagagga cagatgcgta ttgagatctc caaattacat cagagacttg
     3121 gtacaacaat catctacgta acacatgacc agacagaggc tatgacactc ggtacaagaa
     3181 tcgtagttat gaatgctggt gttgttcagc aggtagatac tccaagaaaa ctgtacgatg
     3241 agccatgcaa cctgtttgtt gctggattca ttggatctcc tcagatgaac ttcctggatg
     3301 caaaatgtga agtaaatgga gacaaagttt tattaaatgt aggtccggct aagatcgaac
     3361 ttccgcctgc aaaagcaaag aagcttatcg atggtggata tgctggaaag acggttgtac
     3421 ttggtattcg tccggaagac gtgcacgatg agccaatgtt catcgcatct tctccgaaca
     3481 cagtgatcga agctacaatt cgtgtatatg aaatgcttgg tgcagaagtt tacttacact
     3541 ttgattacga aggagctact atgacagctc gtgttgatcc aagaacaaca gcaagaaacg
     3601 gagatacagt gaaattcgca tttgatgctg aaaagattca cgtatttgac aaagagacgg
     3661 agatgacaat cacaaactaa tctgttttaa acataggata gtagatagta tgatggctgc
     3721 cgcgtgggaa tatacggcag ccattttata ccaatgctac ttgtttatgg aaaatttaca
     3781 gagataaagg ttgacatttt aagaaaaaat cgttatatta atatctgata aaagggagta
     3841 actggcgctt atattaagcg ttacaggttc gtcagtacgg cagtgattct gcccggatcg
     3901 agtatgaaac ggtgagactt ttattgcatg agcgtggtgc agtaaaagtc tcttttttta
     3961 tactttactt ttctgtatga atcgcaaatg aaaaaatggt gaaccctata agaaagtgga
     4021 ggaggaaaaa atgaaacagt attatcagat gggatctgat gagaccagga tgcagattaa
     4081 cggctctttg gagtcgcttt ccagtgagca ggtgcgggaa catcagaaga agtacggacc
     4141 aaatgagctt caggaagaga agaagaaaag catcgggatg atttttctgg agcagtacaa
     4201 ggatttcctt gttatcattc tgattatctc ggcaatcatc tcaggttttc tcggggatgc
     4261 agaaagcgcg gcggtaatcc tgattgtcat cacaatcaat gcaattcttg gaacggtgca
     4321 gactgtgaag gcggaaaaat ctctgaacag tctaaagaag ctctccggac cagaggccaa
     4381 agtagagcgt gatggaactg tcatgcagat cccgtccagg gaagtaacag tcggtgatat
     4441 tgtcattctg gaagcaggag atcaggttcc ggcggatgga aggattatcg aacaggcaag
     4501 tctcaaagta gatgagagcg cactgacagg agaaagcatt ccggtagaga agagcataga
     4561 cttgattgag ggggaggtgc cgcttggcga ccgggtgaac atggtatttt ccggaagctt
     4621 cgtcacatac gggagaggaa aatttgttgt tactggtatt ggtatggaga cggaagttgg
     4681 aaagattgca tccctcttaa agacgacgtc tgagaagaga acaccgctcc aggtcaattt
     4741 ggatcagttt ggtcagaaac tttctatcct tattctgatt ctctgtgcaa tcctcatggg
     4801 aatcaacgtg tggagaggag aatccttcgg agatgcattt atgtttgcgg ttgcgcttgc
     4861 ggttgcggca attccggagg cattgagttc tattgtaaca atcgtacttt cttttggtac
     4921 gcagaagatg gcaagagaac atgcgatcat cagaaaactc caggctgtag aaggtctggg
     4981 cagtgtgtct gttatctgtt ctgataagac aggaacgctg acacagaata agatgacagt
     5041 ggaagattat tacattgcag ggcatagaat gtcaaaggag gaagtctcca cagagcagga
     5101 cgaggtcaga aggctgatgc ttgcaagtat tttatgcaac gatgcaaaga atgtaaacgg
     5161 gcaggagatt ggggatccga cagagacagc tctagtcacc tttgcaggaa actgtggaat
     5221 ggatgtggaa gagatccggc aaaaatatgg aagaatctcg gaagtgccat ttgacagtga
     5281 cagaaagctc atgtctaccg tacatgagac gagtgagggt gttatcatgt ttgtcaaagg
     5341 cgctgtggat gttctttctg gaagaatgtg caaagtacgc agaaatggtg aggaagagcc
     5401 gatcacagat gaggaccgga agcacattgc cagtgtcaat caggatttct ccagacaggg
     5461 acttagagtt ctcggatttg catataagag actggagaca aagacagaga ttgtagaaga
     5521 tgatgaagat gacctgacat tcctcggtct aatctctatg atggatccgc caagagaaga
     5581 gtccaaagct gcggtagcag aatgtatccg ggccggaatc aagccggtga tgattacagg
     5641 agaccacaag gttacggcag cggccattgc aaagaggatt ggaatcctta aagatgaatc
     5701 cgaggcgtgt gagggtgcgg atattgagac catgtctgac gctgaactga aagattttgt
     5761 agaacatatt tctgtgtatg cccgtgtttc accggagcat aagattcgaa tcgtaaaagc
     5821 atggcaggag aaaggcaata tcgtatctat gaccggggac ggcgtcaacg atgcaccggc
     5881 actgaagcag gcagatgtag gtgttgccat gggtattaca ggaaccgagg tagcaaagga
     5941 tgctgcatcc atggttctga cggatgataa ctttgccacg attgtcaagg cagtggagaa
     6001 cggaagaaat gtatatagaa atatcaagag ttcgattcag tttctgttat ccggaaactt
     6061 cggagcaatc cttgcggttc tgtacgcatc catcgctggt cttccggttc cgttcgctcc
     6121 ggttcacctg ttgtttatca atctgttgac agatagtctt ccggcgattg cccttggact
     6181 ggagccgcat agaaaagacg ttatggatga aaagccaaga cagaaagatg aatccattct
     6241 gaccagagat tttttggcta agattgtgac agaaggactt tctattggcg taatgactat
     6301 gattgctttt atgattgggt atacaagcga aggagcactg ctcggaagta cgatggcgtt
     6361 tggaacactc tgtacatcca gacttttcca tggatataac tgtaaggcag acaggccggt
     6421 gttgtttaag aaagggttct ttaataatat ttatctccag ggagctttcc tgatcgggtt
     6481 tgttctgatt acaggagtat tgacaattcc ggcattgcat ggcatattta gtgttacaac
     6541 tctcaatata acacagcttt tcacggttta tggacttgcg atattaaata ttccggtgat
     6601 tcagtgttta aaagcaatcc gttttaaact cagataaagg ggactttata tggaattaaa
     6661 caacgagaat atgaaaaaaa taaggcatct gatcgtattt actgcactga ttattttgtg
     6721 tttctggaaa tatacgattg tatttcaggc ggtcaaattt gtgatcgggg ttgtgtttcc
     6781 tttcattctc ggaggggcga ttgcgtttgt attcaatgtg ccgatgcatt tcatcgaaaa
     6841 caggctgatc caggaaaagt ggaaagaaaa atataaaata gtcaggaaaa cggcaagacc
     6901 ggtcagcctt gtccttacca ttatttttgt gatcggaatt gtgggaggcg tcctctttgg
     6961 tgttcttcca caattaacag gtacgattgc caaattagga tacagtattc aaacctttat
     7021 tcctaaagta caggagtggg caaacgactg gttccacaat aataaggaag tgatggctta
     7081 cgtcaatcaa ttagagttta attgggataa ggtcattgaa gttgcggtag actgggtgtc
     7141 caacggtgca ggaaatattg tcgagtccgg tgtgaccgcg gcggtaaata ttgtaagtgg
     7201 gtttgcgacg ttttttattg cttttgtatt tgcctgctat attctacttc agaaggagaa
     7261 acttggggtg caggcgaaaa aggttctgtt tgcttttgta cagaagggca gggcagaggc
     7321 ggctatcgag gtttgttctc tgacataccg gacattttcc aattttctga caggacaatg
     7381 tatggaggca gtaattcttg gaacaatgtt tgtaattgcc atgagtatat ttaagatgcc
     7441 gtatgcatgg ctgatcggaa ttctgattgc ctttactgct ctgatcccga tttttggggc
     7501 gtttattggg tgcgctgtcg gtacgtttct gatctttatg attcatccga ttcaggcgct
     7561 atggtttatc gtattgtttc tggtattgca gcagattgaa ggaaacctga tctatccgca
     7621 tgtggtaggc aattcggtgg gacttccttc tatttgggtg ctggccgcag tcagcattgg
     7681 cggcagtctg atgggagttg tcggaatgtt gattttcatt ccgatcgtat cggttttata
     7741 tgcattgttt cgggaaatcg tatatctgaa attaaagaaa aacaatataa aaatcaaaga
     7801 tattcttcaa agcgagaaat aatccgcggg attttcacat ataatgtgga aaacaaagtg
     7861 taggagtcga agcgatgttg aattctttat gggcggggat gattctgatt gggatcatct
     7921 tcgctgcatt tacgggcaga ataccggaga ttacggacgc tgccctggac tcatccaagg
     7981 aggcggtgac actttgcatt acgatgatcg gcgtcatggc attctggatg ggattgatgg
     8041 aaattgcgtc gaaggccggg atgattgaga aaggggcgaa gatgctcaaa ccatttgtga
     8101 gatttttgtt tccggaagtt ccggaagggc ataaagcggg cgaacatatt actacaaaca
     8161 ttatcgccaa cttcctcggc cttggatggg ccgcgacgcc cgcaggtctg aaagctatgg
     8221 aggagcttgg aaaactcaat catggaagcc ctgtggcgag taacgccatg tgcaactttc
     8281 tcattttaaa tatttcttcc ctccaattaa tcccggtgaa tgtcattgcc tttcggagcc
     8341 agtacggaag tgtaaatccg actgcgattg tgggagccgg aattgtagcg acagcaatca
     8401 gcaccggaac agcgattgta ttttgtaaaa ttatggatcg gaagaagacc cggaaaagtt
     8461 aaaacataga gaccattcct agcctttatc ttactctcct catttatttt tttataatat
     8521 gatataattc ttcaggaaag tatcagattc agagcgagaa aaagagcagt tctgaggaaa
     8581 gttgaactgt tctgaatgtg attctttcca attgtaccaa gccgccgcgc cagcggcttg
     8641 gtacaatata tgtatatgga gaaaatcgca ttgaaatcca atggaaaatt cgggattttt
     8701 gcaatggata gttcacatta cgttttagcc tattatttat agaaggtatt aaaaaatatg
     8761 aaaggattgg gatgtgtgta tatactgatt tagccgaaaa aggttaacta attattacga
     8821 acagagatgg cactaatatt ataaaggaag tcttttgcgt tacggaattt aaacattcaa
     8881 aaaaactttg aaaaaaattg ggaaaaccga aagatttttt gttttcgttc gtctatataa
     8941 tagagagata taaaagaaag aaacaggcaa aactagaaca ccatgggaga aaatagtgtg
     9001 aaagaaaata aattgagcca aaatcgtgga atagacacaa aaaacaggct cacatagtga
     9061 accaattaaa aggatattcc cacctgagta gagaggcgca cctgcacatc aataagcaaa
     9121 cgggtgggtg catctgatac tgatgccggg atgcctttat aatcaagtct attgtaacac
     9181 gaaaacttga aggtatgaaa cactttaatt actatttgtg ccgccaactg aaaggcggcg
     9241 gaatggagat taagatgaaa aaaatagtca ttaatgtggc gaagaagagt gtaggaaaga
     9301 gtattccgtt tagcatgttc aaagtcaaaa agcctgataa tttcgaagaa atcataaaga
     9361 gagtagaaga agaaagaatt aaacaacgtg ttaaaaaatt ttttacaaaa tcaaagcaat
     9421 ttaaaaataa tattataagt tttatgaaaa tgctattttg gtcgatattt ttggaaaata
     9481 tcttacgcca tatatactta gtaattaata aaagttgaaa agtcctttaa gtcaaaaaat
     9541 aaatcaaagg acttccatat gaaaatgtca tatattgaca aaactaaaaa aatagtattt
     9601 agtactcatt ctatgtgtat tgaattcaga atattaaggg aagaggttgt cttgcgtacg
     9661 atccagatta ggattttgtt ttatagtagt tttaccttta gtaatagatg gaattattat
     9721 gcttttcaaa agcataattg aatgtattag gagaggggca tattaactat acttgcaaca
     9781 ctaataccat tgcttaagcg aagaggttgg aagaagcggt tttgaagatc ataagaaaat
     9841 attttagttt tagtatttct gttgaatatg aagaggaatg gataacagca aatcaaataa
     9901 aggaaaaatt gcggaaaata aaagaaaata ctggaaacga aaaaccaatt ttaaaagtgg
     9961 aagaatctac tattaaaatg aaaggcgatg ataaataaaa atgagatata aaataaaaac
    10021 aaatccagag gaatttttgg tttctgaaat tagtaatatt cagactactt tatcgcagga
    10081 taaatataaa atttttttat tggaaaagaa agggtattca acatttgatg ccattgcttc
    10141 cttgtctagt tacttatcta tagatcaagc tgaggtgggg tatgcaggat taaaagatga
    10201 ggatgggata acgaagcaaa agattagtat tccttctaat ttaattaatg aaaaacatgt
    10261 cgaggctttt aataggaagt ttatagacag agataaatat atgcatataa catatatagg
    10321 ttttactagg aaaaagattg cgataaataa tcttttgggg aacagtttta aaattcgtat
    10381 aagaaatatg gagaagagag aagttgatta tcttttaaaa gtcagaaaaa ttaatttaaa
    10441 ctatgtcaac tattatggca tacaacgatt tggtctaccg gataaagaga aaaaaacaca
    10501 tttaataggg aaatttttat tacaaggaga ctatgaagaa gcatttaaat tggcatgtac
    10561 agttgaggat aattttgata aaagcataaa ttcgtcatat tattttgaaa aaatggattt
    10621 tcgaaaaaga agtttttatt acaatgcata tagttcctat gtttttaacg aatctctttc
    10681 aaatttagtg aaagagtata gccaaaatgt tcttaatgag gaagaaaata taagctttta
    10741 ctttcctata aatgagaatg gaatattaaa tatttgggca cataaaaaaa tgctacccat
    10801 aaaaaggtat tactatgatg aaagtaataa tttaactgag aaaaggtcta gcagattttg
    10861 tataagtaat tcggtcgtta aaataataca agttcaagaa gatgaaatat atcataagtg
    10921 gagtgcagat atcgaattca tgttgccatc gggaaattat gcaacagttg ctattatgca
    10981 actggaaaat attatgtcaa atcttttgga gaaaagtcat gaaaaattta ttgatttctg
    11041 attttgatgg aacattatat cataataata ttaatattta taaagaaaat ttattattta
    11101 taaaaaggtg ggcaagctct cctgataatt atttttcaat agcaacaggt agacttgcta
    11161 gagagatata taatatatgc actaaagaaa acattttata cgagtttatt gtatgtaacg
    11221 aagggagtag cattatatat aataaatttg ataaaaatga tatatttaca tttacagcaa
    11281 gttcatgttt gcaaatttat agatcattaa taaaacttgt tgaatcgaaa caactcaagt
    11341 actttttaga aacggatgga gagtatggat ttattgattg ttataattta gatgataaat
    11401 gtagaaatat tgttagagaa aagaaaaaaa caaaatataa ttatttaacc ttcaaagata
    11461 aaaattcaga tataaaaact agtattttta tattaaagat tttagatatt tcttatgttg
    11521 atttagtaaa aaaaatatta gagaaagagt tgggtaatat ttgtagtatt tcacaagctt
    11581 atacatatat ggcactgata cctaaaaaag cgagtaaagt agaggggatt gcaaaggcgt
    11641 taaaagataa taaaaggaaa tttgatagga tattaatggt tggagatagc cgaaatgata
    11701 ttgaaagttt aagaaagtat atggggttta caatcaaagg atctgaagct gaaaagattc
    11761 ttccagaatt accttcggtt caatacctaa atgagataga gaattatttg taataggtta
    11821 gaaaatcaaa gagggggaat aatataatgg aaaaatattt agccttttca aaacttacat
    11881 tatcatcgaa ggcaatttat agaaaagctg tgctttttga tattttcggc attgctttgt
    11941 caatattgct atattttttt ttgtggtcaa aagtttatgc tggacatgac acaatagcag
    12001 atttttcgta ttatgaaatg gttttttata ttataatttc gcaaacattg gcatcacagt
    12061 tttatggaag tagtggtatt aataacacct tatctgagtg gatatatgaa ggaaatatta
    12121 gtatagaaat tttaagacca gtttctttaa ttgctaattt gttcgctcgt aaactgggag
    12181 atgtgaaatt ttttctcatg actaaagcag gtccaattat aataatagtt atgtttggat
    12241 tttatggatt aagaaaagtg gacatgccag gtattataaa tatatcgttt tctgttttgt
    12301 gtttggtgat atctttaata atagtctttt tcttcgaatg tatggtaggt tttatttctt
    12361 tttttaccat gaatagtcat ggaataagta ttgtaaaaaa tgctttaatg ttactaatgt
    12421 ctggtagcgc aattccatat tttatccttg gaaatagaat tggactatgg ttaaacttat
    12481 tgccatttag tgggacaatt tctataccta tacaaatatt attagggaaa tacagcgtaa
    12541 tactctcttt aagatatatt tgtgtgcaac tactttggag tttggtgttt ggagttataa
    12601 cattattatc ttatcaaaaa gttatcaaaa gagtagttgt tcagggagga tgatttttgt
    12661 tgaaaagaaa aggctttttt tatttgagct tagggaaagc atatattcgt attggatttc
    12721 agattttgaa acagtatcca ttagatatga ttctgtcctc attatcgatg ctgcttaggg
    12781 actccgtcaa tttcgctgct attataacgg ctataagcat tatgggagac atggggaaat
    12841 ggaaggtgta cgaaattgta tttttatata gttttagtat gatgatcgaa gcatcgagtc
    12901 aaatcttttt tgatgccatc tggtatttgg ggtctatgat aagaaagggg aaattggata
    12961 ttattcttat tcgaccggca ggaagcttgt ttcagttttt tggaagcaaa atacaattgc
    13021 aggcaagttt aagcttttta gtgtcatttt ttgtatttgt acttagcatt gcaaaaatgg
    13081 attatataat taccggaaaa agtataattt gttttatatt tttcttgctt ttttgtactc
    13141 ttttaaattc ttctatttta ggaattttta actgtgcgaa tttttggctg ataaatgcag
    13201 gagaaatatc tagtatagtt tacaaactgc gagatttttc caaatatccg ttatttttat
    13261 tccctgtttg ggtaattaat ataataacct atgtgattcc gtttgcgacc acaagttatt
    13321 atcctacgac attcttgctt tttggagaaa acaataatat tatcggattg ataattggtg
    13381 aaacaatatt ttttttcagt gtttttgttc ttatttggag aagagggata ggaacctatt
    13441 caagtacagg tacttgaaat ggcaatgaat tttaataaaa aggggactca taacatggta
    13501 gaagtaaaag atttgtataa agaatatagt ataaaaaaaa gaggagcaac ccattatttt
    13561 ttgaaaaggt cagagagaac tataattaaa gcggttgacc atgtgagttt tattgtgaat
    13621 caaggtgaag ctgttggcta tgtcggtccg aatggaagtg ggaaatcgac tactataaaa
    13681 atgttaacag gtgtattgca accaacttct ggagaaatcc gagtaaacgg cattgatccc
    13741 agtaagaata gaatagaaaa caataggaat attggagtcg tgtttggaaa tcgttcgcag
    13801 ctatggtggg atgtaccaat tattgagtct tttggatttt tacaaaaact atatcaaatt
    13861 cctgattcga tttacaaaaa taatttggag tattttgttg ataaattaga attacaaaat
    13921 attattaatc gaccagaaag acagttatcg tttggacagc gtattagatg taatatagtg
    13981 gcagcttttt tgcataatcc ggcagttgtg tatttggatg aacctacgat tggattagat
    14041 cttgaatcaa aagagcggat gagaaacatt attaataaaa taaataaaga gagggaaaca
    14101 acgatattta ttacttcgca tgatttgttg gatatagaat tgctttgtaa tagattgtta
    14161 attattgata atggaaagat cattactgat actgatttcg ataatatgtt aaaaatttat
    14221 aataggaagc aaatttttaa atgtagagta attgataaat taatagatat cccgatgaat
    14281 ttgaaaaaag atatcaaagt aaggagatta gaggggaatc atctagaagt tgagtttgat
    14341 aatcaaaaaa tagatataaa agaattactt gaagaacttt caaggtatgt ttctttctcg
    14401 gatgtttcta tagaaagtat gtctatagaa gatattgtaa aaaaaatatt gataagaaag
    14461 gaacagtaga tatgcatttt atagtactaa tgatgtcaat ttttctatta attggaatca
    14521 taagaggcat attaagaata aaagatacga aaacgtcgct gaaaaattat actgataaac
    14581 taaaaggcaa tatcgtaaga gagttttctg atattgatat tattattcca gtttataacg
    14641 aacaaaaaat tattaaacag tcaataaaca attttttgcc attaatagaa aaaggtgtac
    14701 gtgttacata cgttacgact caaaaagaag agagttctcc tacgactaag gaactattga
    14761 gaaaatatat agcggaatat catctcgtgg ggaagtgcat gattgttgat tatcctagta
    14821 aaaaaggtgt gatgtcacac caattaaatt tcgcaataaa acaaataaaa gagaatagga
    14881 ttatttgtat atataatgtt gactcaagga ttgatataag aactattgag tacatattgg
    14941 aaaataagga tatattatct gaaggagtat ttcaacaata tagtttcttc tcacataaaa
    15001 aagaaaatgg agtattaata aattcagcaa ttgcatggca aaacagatgg acaatcggat
    15061 atgaatatcc aaaggcgttg aaggaaaaga atgggaaaaa atggtacaat tttaactatg
    15121 taattggtca tggcttaatt tttacaagaa gaatgtacga taaatttggt ggattttcag
    15181 aagaagaaat taatgaagat aatgtgctgg ggtataagtt acacttgaaa aatataagaa
    15241 tcaccccggt tcctatcttt gaaaatgctg attttgcttc gagtttggga atctatctaa
    15301 agcaacagtc aacatggttt aacgggccgg catatgcttt taaatacgcg tataaatatt
    15361 atcaaaatga aaaaaacgat ttaggcaata taacattagt gtcaatttcc aactttaaaa
    15421 acgctttaaa ttgggctctt ttacctattg gattggatat atgtttaata tatttagttg
    15481 ttaatagtca aatacttact tttatattat tcctattgct agttgtgata tatgctacag
    15541 tattggattt tcttagtgaa aaagttttgg tatcgcatgg atatctaaaa aagcaaaata
    15601 aagcttctta ctttttcgat acttgtttcc ttttagggat acattttgtt ggtcctttaa
    15661 ttacaattac aaaaatttta tttagaaaga attctatatc taacaagtat aaaacagaaa
    15721 aatcataagg actaaaaggt tttgaataaa ttaggagaga aaatatgaaa aataaaaaga
    15781 aaagcatttt attttttaat gcaagaagaa gagtaaatat ggcaaaaaaa atgattgagt
    15841 atttaaagaa tcggtatacg gaataccaat taattagttc agatacagat tatttggatc
    15901 ccattagcta tgtaacagat gactttgtag tattgccttc aatagaaagc caagaatttg
    15961 aaagtgtatt tttggaagtc ataaagaaaa aaaatatagt tggtataata atgtggaatg
    16021 ataaagactt cgcagctata gaaaaaatgc gtttgaaaat cgagaaattg ggatgtaaaa
    16081 ttcttatgcc taaagagaaa aaatacgaaa tatgcaataa caaaatgtta acagataagt
    16141 ttttacggac acatgctctt tacactccac aaatttatga gaatccagat aagattaaga
    16201 aatatccagt aattataaaa cctaagaatg gggctggatg tgtggatgtt tataaagcgg
    16261 ataatctaga agaagcaata gtcttttata aaaaagtgcc aaatcccatt attcaagagt
    16321 atattgaagg agttcattat acagtagaca cgtattttga tcggtgcgat ggtgtgttat
    16381 gtagtgtgcc aaggaaaaga ataaaagttc gtgaggcaga agtgatgatt gccaaaatag
    16441 aaatgcaaga agaggtaatg aatgtagcac aacaactaac gaaattcttt tcggaagaag
    16501 gcccaatgaa tatacaggta attttatctg ttgatgatgg aaaaccatat ataatagata
    16561 ttcattgtag aattggtgga ggaagcgaat tgtcaataga aggtggagtt ccattacatg
    16621 aatggataat tgatgatatt attggagggg aaaaaagaat acaatatatt gttaatgacg
    16681 gattgattat gacaagatac tttgatgcct tttttataaa tgaaaatagt agtataaaaa
    16741 ataatattaa aagataataa ggagtaagta tggataagag taaattgatt ttaaaagtta
    16801 aaagttattt aaataacttg ccagaggaaa tttttgataa gaattattta acaacagcat
    16861 ctgtgttttt gtcgggttca acaggttggg gaattaaaga aggatttgat gagatagcag
    16921 attgggattt acatattatt ttagacaata ttagttatga acattataag aaaaagtacg
    16981 gggagcatca tgttatagat gatcaacaac atcaaccaaa agtttttgct cagattagaa
    17041 gtataagctg gctcgatata agattacaaa atattcaaaa tgctgattgt acatacttat
    17101 ggatatataa taacgctttg ctaatacaag acaatttaca tattgaagag aaaattgacg
    17161 aatggaatag tttctttaaa aataatattg aagaaatttt aaaatatcat tttgttacat
    17221 ttgctgttag acgattagat gcggtatctg ctataaagag aaatactgtt attggaacta
    17281 atatttctaa aatagaaatg ataaaggaag cattgattat catatcttta cttcatcagt
    17341 ctccgtatcc atataataaa tggatggaaa agtatgtttt gcaattgggt gaagacggag
    17401 aaaagtgtgt ggagctatgt aagagatgta ttttcgaaat aaattttgaa agtaatgcac
    17461 gtgttctaaa aaattttctt gtggcagaaa taaaaaagca agttggcaat ctaccttggc
    17521 ttgatgaatg gtggaaatat aatgaaaacc caccagtggt gtatatcaga taataggagg
    17581 agatcatggg atattgggca tcttttattg ctttggattt agaaaaaata gaagccaata
    17641 aaatagtaag ggaaatgaag gataagttaa aaaaatataa taatattatt tcatggcata
    17701 atccaaatta tttgcattta acactaaatt attttgattg gcttgaggaa acacaaatag
    17761 gaataattga aaaactgttg acagaagtac aaaataaaat tccagataag attttaatta
    17821 aggatgagat tatttgtatt ggtggaaacg aaaataaata tgtagcttta gaaatagata
    17881 aaaaaaattt gattccgtta aaaaaagaca tggatacttt attgattaaa caccgaattg
    17941 attataaaat tcaggaattt aggccgcata tatcattggg aagaattaag aaaggaagtc
    18001 taattaagct aaaaaatata gaacaagcta taggggttaa aggaatttct ctatatgaaa
    18061 gtaaggaata tagtatttca attatgagta gatgtaagca gtataataaa aagtaaagta
    18121 atttaagaaa tgaaagatat atgttatggg aaaaattgtt gttacaacag gcggaaatgg
    18181 cactttgggt aaaagtgttg taaagaaatt aataaaagag gggtattttg ttatttcttt
    18241 agtgcataca cttagtgatg agaaaaaagt aaatatatta gaagtagaaa ctgatctttc
    18301 agatattaac gccatacaaa gaagtataga gagagtaaag ctaattatta gtaaaataga
    18361 aatagaaagt ttaagtatta tacatatggc tggtatttac gagaagcaaa agtttcctgt
    18421 aaagataggt gatatgcaaa aatggaatga aattttttgg gttaatacgt tttcttttta
    18481 tttcatagtt tctgaattgg ttgatttatt taaattaata agaagaggta atataattgc
    18541 agtatctagt aatctgacga aacttgttaa caatgatact gcagcatata taggggctaa
    18601 agcagcatta gaaaaaatta ctgttcaatt agcatatcaa ttgggagaat ataatatatg
    18661 ctgtaatatt atttgtccag gctttttctt cagtgctatg agtaataata ttccattaaa
    18721 ggttttagaa gaaattaaaa agaatacacc gctaaatcaa gtggtaacag aagaaacaat
    18781 tgctacagtc attttagata ttttatgcag taagaattta gggatgactg gacagatcat
    18841 tacggtggat ggagggaata caattgggtt tgcaaagaat taaatttcct aaaattatag
    18901 agtttcaaac tcagacatta tgtaacgggc gatgcacaat ttgtccttat gaacaggtaa
    18961 aaaaaataat cccatatttt agaatgtcag atgaaaaagt gaacttgtta ataaaagaat
    19021 tagcagaaca taaagaagat atagaaagag ttgtaccgta tctaaataac gaaccatttt
    19081 tagataaaag atgtatatct atattgcgaa gactaaagaa gaatcacttt atagagttat
    19141 caacaaatgc atcattatta agtaaagaag ttgcagaaac tattgtaaat gaaaagctag
    19201 ttgatgattt tagaattagt ttttttggag ggacgaaaga aagctattcg aggctaatgc
    19261 ggggattgca atttgaaaag gttgttgaga atatacagta ctttttagca attaatcgca
    19321 gaaatatcaa tacacagatt actatgatat tattgccttg gttagatatg aagaagagta
    19381 ttgaggaagt aaaaaattta tttccgaatg aaaatattgt tacctttggt tatcttgata
    19441 gggcggggaa taacaaaata ttcagaaata atttatgtat gtatgaaaaa gataagatta
    19501 aattgtgcgg gtgtaatctg gagcgtccat ttgaaagaat gtgtattatg tctaatggaa
    19561 atgtaatttt atgttctcag gactggaaaa atgaaatcgt tcaagggaat atattcgaaa
    19621 cttccatatc agaagtatgg aatagcaatg aatcaattaa tataaaaaaa atggtaaagg
    19681 gagaggttga aagctccgac aattttttgt gtaaacgttg taaattggca aaaataaaag
    19741 tagaagatag aatggttttg aattttgcgg gcgataaata tatggatgaa tgggatagaa
    19801 aaagatatga atacaaagat taatatttta atgcttacgc ctgattatac tcctgtatat
    19861 agtggggtag ggacacatgt tgtcaattta tgtaaaaatt tgtataaaag taaacaatgc
    19921 aatatttatg tagtagttat acgtatggct acaatagaag aagcaaaaat aaaaaatagt
    19981 acaaagagat taaaaaaaat agtaaaagaa ggaataacaa tatttgaagt ttttattggt
    20041 gattttacta aaagatatta taaacgtttt ggaaaacagg ctaatataag ggaattagac
    20101 tacattttgc cagacggaat gctactagaa gaaattgttg agtgtttaag aaaagaaaaa
    20161 ttacatatag atattataca taatcatagt tttcataatt cggtattttg caagctatta
    20221 gcacaatatc tagattgtcc tcttatattt acaatacata caaattataa taacaaaaag
    20281 catcaaaaat cattaaaaaa atatctatgg gaacacagta ttaaaagctc caattgctgt
    20341 attttgttga gtaatattat gaaagaagag attggaaata tattaggaga cataccatgt
    20401 gaagttattt ataattctat agatatagat gctcaaaaca gttatattga aggcgattat
    20461 gaaagaagaa acgatttatt atactgtgga cgattaagtc gagggaaaaa tgttctacaa
    20521 ttatttgaag ctataaaaga actaattact attgaagaat ttaaagatat aaaattgtat
    20581 gtggcagggg aaggtggaca gcgtggaaat ttagaaaagt atattaaaga aaatcaatta
    20641 tcaaaaaata ttattatgct aggaagaatt acacaaaaag aattaaatga gtactataga
    20701 caagtccgtc taacaattat tccgagtaaa agagaagttt ttaacacggt cgctttagag
    20761 gcaagtagta agggatgttc gcttctttta tctccgttag attcttttaa agaaatgttt
    20821 ggtgaaaata ggatcatttt ttttgataaa ttcaatgcac agagtattta cgaaaaaata
    20881 aaaaaatact attttgatat agaaacttta gactatttcg ccaagaaagc ttttcgacat
    20941 gtaaaaaaat gctatgcttg ggaaaagaat tgcatgaaaa ttatgagttt atatcaaagg
    21001 gtaattagtg aatatcaaag agaggttgat attgaatcaa g
//
