LOCUS       NZ_JADNBL010000006     21083 bp    DNA     linear   CON 12-JAN-2024
DEFINITION  Bifidobacterium longum strain D59t2_181005_F6
            NODE_6_length_124380_cov_26.1225, whole genome shotgun sequence.
ACCESSION   NZ_JADNBL010000006
VERSION     NZ_JADNBL010000006.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bifidobacterium longum
  ORGANISM  Bifidobacterium longum
            Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
            Bifidobacteriaceae; Bifidobacterium.
REFERENCE   1  (bases 1 to 124380)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Quantification of discrete gut bacterial strains following fecal
            transplantation for recurrent Clostridioides difficile infection
            demonstrates long-term stable engraftment in non-relapsing
            recipients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 124380)
  AUTHORS   Aggarwala,V. and Faith,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2020) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, 1470 Madison Ave, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 29.17x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 03:10:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,967
            CDSs (total)                      :: 1,906
            Genes (coding)                    :: 1,845
            CDSs (with protein)               :: 1,845
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 19 of 61
            Pseudo Genes (incomplete)         :: 48 of 61
            Pseudo Genes (internal stop)      :: 3 of 61
            Pseudo Genes (multiple problems)  :: 9 of 61
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:10:38
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 61796
            Orig. end                         :: 82879
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JADNBL010000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21083
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="1"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..21083
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21083
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:11083](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11083)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    complement(325..340)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.61"
                     /tool="antismash"
     misc_feature    404..417
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 19.86"
                     /tool="antismash"
     misc_feature    complement(405..418)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 20.94"
                     /tool="antismash"
     misc_feature    complement(406..419)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.1"
                     /tool="antismash"
     misc_feature    454..471
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 21.41"
                     /tool="antismash"
     gene            501..3395
                     /gene="secA"
                     /locus_tag="I2A13_RS05425"
     CDS             501..3395
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006605 - protein targeting [Evidence IEA]"
                     /GO_process="GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /GO_process="GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /gene="secA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410781.1"
                     /locus_tag="I2A13_RS05425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="preprotein translocase subunit SecA"
                     /protein_id="WP_007055154.1"
                     /transl_table=11
                     /translation="MVDIIDKALRMGEGHQLKKLENVAKAVNALEDEISALSDEDLKAQ
                     TPKFKQEIENGKSLDEIMPEAFATVREVSKRTLGQRHFDVQLMGGAALHWGNIAEMKTG
                     EGKTLVATLPTYLNALEGKGVHVVTVNDYLASYQSELMGRIYRFLGMNVGCIITEQKPP
                     ERRKQYNADITYGTNNEFGFDYLRDNMAWEKADLVQRGHHYAIVDEVDSILIDEARTPL
                     IISGPAEGDVTRWYRQFAKLVLKLTRDEDYDVDEKKKVVGILDPGITKVEDFLGIDNLY
                     EPANTALIGYLNNAIKAKELFLRDKDYVVTQGEVLIVDEHTGRILPGRRYNEGLHQAIE
                     AKEGVEVKAENQTFATITLQNYFRMYDKLAGMTGTAETEAAEFMNTYKLGVLPIKTNKP
                     MIRKDQDDLIYRTKKEKLAAIVKDVAKRHAKGQPVLLGTASVESSEVVSTLLDVAKIPH
                     QVLNAKQHEKEAAVVAVAGRKGAVTVATNMAGRGTDIMLGGNVEFLADAKLKSEGYSPE
                     DTPEEYEKRWPGTLNEIKAQVKDEHEEVKELGGLYVLGTERHESRRIDNQLRGRSGRQG
                     DPGESRFYLSLEDDLMRLFNTQLVAQVMAKGMEEGQPIEAKSVTKGVRTAQKAVESRNY
                     EIRKNVLKYDDVMNKQRTVIYSERQAVLKGEDIHKDILRFISDTVESYIKGANKGSEKP
                     KDWDWEGLFKALNTVIPTKVDEDEVRKIVGGLKGAKAVEAVRDLIVEDARQQYGEMEET
                     IGETGLRDLERRVVLAVLDRKWREHLYEMDYLKDGIGLRGMGQRDPLVEYQREGYQMYN
                     SMIEAIKEETVQLLFHIDIKQVATTDEAVDEVEETAESADTIAVASGPDENGESVVEAA
                     EGEVEEEDEDTDAKQAIAESAAASGAGESTLPVAGPAPISHAEGKVPVSKRPKSEELKT
                     PWADGRTFPGTGKNAPCPCGSGRKYKMCHGQNEK"
     gene            complement(3706..4059)
                     /locus_tag="I2A13_RS05430"
     CDS             complement(3706..4059)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /locus_tag="I2A13_RS05430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_010080769.1"
                     /transl_table=11
                     /translation="MPSPFGGLTSIGVDETSYRKGHTCITVVVDHERHRAIWAHDGYGR
                     DVFGLFFQTLTPEQRASIRVVAGDGARWIDSCVHEWCPNAERAPDGFHIVSWTSGAPDN
                     PRKQQKPLFCAIP"
     gene            4194..4580
                     /locus_tag="I2A13_RS09835"
     CDS             4194..4580
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="I2A13_RS09835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_229033791.1"
                     /transl_table=11
                     /translation="MDRLPGSAHGTEATTTPHAAALVMPAAPPSASGASEHTRTWTHPD
                     LEHGFGTRRRIRDPRILCNHAFDVRQSGEWAFAQAFFVIHFFGRKTEYAGTPLTLTKPT
                     ARRRTDIHENSNSAIFCNMEDSGP"
     gene            4649..5695
                     /gene="trpD"
                     /locus_tag="I2A13_RS05435"
     CDS             4649..5695
                     /EC_number="2.4.2.18"
                     /GO_function="GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582794.1"
                     /locus_tag="I2A13_RS05435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="WP_195242520.1"
                     /transl_table=11
                     /translation="MAEITWKSILTKLVGGDHLTAEESEWFVDDLMQGNANPAAVGAAL
                     AMQQQLGLTADEVRGAAKAMVSHAVPLNVSGETTDIVGTGGDGFATVNLSTMGSVAAAA
                     AGVKIVKHGNRAASSKCGAADVLEALGLPLDLKPEAVGEVGDEVGITFAFARTFHPAMR
                     FVGPIRAALGIPCVFNILGPLTNPANLAHVAIGCANRKVSPIMAAVYASRGQSGLVYTS
                     HEGLDELAPTGPVSVWEIRDGKVTESEFDPTVDLGLTKITVEQLKGGVPDVNANAFKDF
                     LAGKDIPSRTTALLNAASAIVADGNLVGNGTLAERFAEAYKLAEETVDSGKAEALFDKW
                     IETAKSKA"
     gene            5741..5929
                     /locus_tag="I2A13_RS05440"
     CDS             5741..5929
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007054484.1"
                     /locus_tag="I2A13_RS05440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_131205554.1"
                     /transl_table=11
                     /translation="MATVMAAAALCVTAACGTSVGAADVPVVPAKGDVTIRLNWWGGDA
                     RIKAMIAELQESIDFNS"
     gene            complement(6083..6778)
                     /locus_tag="I2A13_RS05445"
     CDS             complement(6083..6778)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007055163.1"
                     /locus_tag="I2A13_RS05445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_187400774.1"
                     /transl_table=11
                     /translation="MTENQNSNADGERPTPTPGNGQDSGTSSKSPTPGAAGQATNGADT
                     PQSYDANDLDAAWAAFEAEHQADLHDVASSRQAKKFEKQAKKREKEALLSVDDLDIGSF
                     TDDARPSRGGKKGKQSGPRDFTGSSWLDTDNVMDAYDDGGFTPPNPDLGPVKASTVLLT
                     ALLIIGLVGEAVMIFIPGLNAIPLLGALLNILFGLGVILGLGGLVAKILNRKDRPGDQG
                     GYYDDGARV"
     gene            6839..7540
                     /locus_tag="I2A13_RS05450"
     CDS             6839..7540
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1141: acyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007054486.1"
                     /locus_tag="I2A13_RS05450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="WP_131238721.1"
                     /transl_table=11
                     /translation="MLYWFFVKGLGPIAHHRLSPAVSGLNNVPREGGAIIAANHLAVID
                     DALIPITCPRMVHFMGKAEYFEGKGLKGKFKKWWFTSVGVFPVDRSGGSKSLGALEHAR
                     EIIEDGHLFGIHIEGTRSPDGRLYKGHTGVARLALETGCPIVPTAIIGSDKLQPIGTSI
                     PKKGKTKVLYGKPIEVAKKPADEITHDDLRSLTDRVSAAIQKMSGQEFVNEYAQKVKAE
                     LKAQQAAEEAE"
     gene            complement(7584..9857)
                     /locus_tag="I2A13_RS05455"
     CDS             complement(7584..9857)
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410787.1"
                     /locus_tag="I2A13_RS05455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="protein kinase"
                     /protein_id="WP_195242521.1"
                     /sec_met_domain="Pkinase (E-value: 3.7e-53, bitscore:
                     173.2, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSENEPAQMIEGRYRIVRNIAEGGMATVYEAVDERLGRTVAIKVM
                     HTQLAKGPHREQFVERFRREANSAASIANPHIVQVYDTGEFNGLDFLVMEYVHGVNLRH
                     EMNAQGTFSVRETLRVVAETLDGLASAHRAGVVHRDIKPENILINDRGHVQITDFGLAK
                     AASQATLSSTGMLLGTAAYLAPEMIENNQATAQGDLYSVGIMAWEMLTGKVPFDSDNPV
                     TLVFKHVHEDVPSVATVCQGIDPSVAAFIAHLTARQVDARPTDGAAAAEELSQLAAKLP
                     LEAWQYRLHAEPIGGDHTDATAAALVGNIAEQAPLTGVPAGAATFEPSVPAFLVDDAAS
                     NTADTGGAADVNPPAPPVAPTTALDSSMLADASAPHKTQIMAQSGSETQVLPQAGDAFT
                     RALALSDEPDVASNGTGPKKQRSKKPLIIVLVIVLVLAAIGGTAGWWWFAGPGSYWSVP
                     KPDDVTCDANAGTECSLAGADWATYESTLKALGIPYKTHKEYNDDVAEGKIISSSVNKT
                     KAVVNSRISKRANQELTVVVSKGVRMATIPKDILDANSANGKDPLNALKRAGFDNVKHD
                     ESKDEYSMDTPQGVALTISPDPGTAAKHNDEVTVTLSKGPMPVTMPNIVGKTQDEMQAA
                     LGELKLTANVTEQYDDKVEAGQVISASQEAGAQLKWGDSVDVVISKGPEMATIPSGLVG
                     KQESAVTKTLEALGFEVKTDKVLGGLFGTVRTVKSGDTDLSNGGKIRLRDANGNPTVIT
                     LAIV"
     gene            complement(10001..11083)
                     /locus_tag="I2A13_RS05460"
     CDS             complement(10001..11083)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512156.1"
                     /locus_tag="I2A13_RS05460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_007058720.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 4.3e-48, bitscore:
                     156.5, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCTTANNREIIEPRIIQLVRELTAAPATDEVADALKPVMEQVVDQ
                     AASSSQGGKRLRALLALDAFDILAGDVTPDRRDAMIDLACAIEVFQTAALVHDDIIDES
                     DLRRGRPSAHRALEQAVHSGAIGRGLGLMLGDILATACIEITRRSASRLPNTDALNEAF
                     LTMQREVEIGQVLDLAVEMTPLSNPEALANASLNVFRWKTASYTTIAPLLLALLAAGES
                     PDQARHCALAVGRPLGLAFQLADDLLDVVGSSRNTGKPVGGDIREGKRTVLLADALSAA
                     DTADKADLIAIFEEDCRNDNQVARTIELFTSTGALDRSRERIAALWGESRKAIAGLELN
                     SEAQRRLTEACARFVPESLR"
     gene            11290..11952
                     /locus_tag="I2A13_RS05465"
     CDS             11290..11952
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007052659.1"
                     /locus_tag="I2A13_RS05465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4192 family protein"
                     /protein_id="WP_007052659.1"
                     /transl_table=11
                     /translation="MNNNTPHKPRKSRHAKRHAAEQTEPFGFDELETRSQEFRNDRRLR
                     GAQQANADWVAEPLDEWLSCLDAGNTDLGRETMAAFAVGMRETLSIRDALILSLVIDES
                     RCPKSWLMEFAARPHLARTRTRMGELLTCAFEDEGMMPDKERCHAGIGMLLDIAEASPV
                     PYCIQPLAVIAYTLWWLGDPRAMVFALRCLLLDEDCSLAAMIFSAADRGVAPAWCSG"
     gene            12155..13579
                     /locus_tag="I2A13_RS05470"
     CDS             12155..13579
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007055162.1"
                     /locus_tag="I2A13_RS05470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_007055162.1"
                     /transl_table=11
                     /translation="MATKETTATKQTAESSEETETKKKASSTTTARKTSAKKSKSAEPS
                     KKTTRKSAAKKTAKKAKDDELVKDEDTQVDDELDDDAEVNDDDLDVDDLDEDLDDVDED
                     DADDEDLDEDDADDDDVEDDEDEEEAKSKAPEQPKEKGAYVVSDTDDEEENIIPSGNPK
                     RRVIAAGATADPVKDYLKQIGRVSLLNAEQEVDLSERIEAGLYAQHLLDTESEGMEFKR
                     KRELKWAAADGKKAKDHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNLGLIRAVEKFD
                     WKKGFKFSTYATWWIRQAITRAMADQARTIRVPVHMVEVINKLSRVQRQMLQDLGREPT
                     PDELARELDMPVEKVQEVQKYGREPISLHTPLGEDGDSEFGDLIEDTDAIAPSDAVAFS
                     LLQEQFKQVLETLSPREAGVIKMRYGLEDGQPKTLDDIGRVYGVTRERIRQIESKTMSK
                     LRHPSRSQTLRDFLDQ"
     misc_feature    13613..13626
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.19"
                     /tool="antismash"
     gene            13637..15955
                     /locus_tag="I2A13_RS05475"
     CDS             13637..15955
                     /EC_number="5.6.2.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783099.1"
                     /locus_tag="I2A13_RS05475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="WP_013410790.1"
                     /transl_table=11
                     /translation="MPKEEYGAKDLAVLEGLDAVRKRPGMYIGTTDSQGLMHCLWEIID
                     NSVDEALAGFCNDIVVTLHTDGSVEVADNGRGIPVDKEPKTGLSGVEVVLTKLHAGAKF
                     GNSSYNAVGGLHGVGSSVVNALSSRLDVEVDRDGKTHHMTFHQGHPGVYTDADPANPSP
                     DSPFKRTRKNRPTELEIIGKVSPKTTGTRIRYWYDPEIFNKTAEFSYEQLIDRVRQTSF
                     LVPGLKITIIDENVPETAATDTVEATDDTTVEPAQDQAPALDVSSSDAELFDDSAESQS
                     DDAESPAEEDFSLTAGDQVVDGAFGEQPAHPRVVEFLHTGGVKDFVDFLSKGEPISDIW
                     RIQGEGTYKEETQAVGEGGELHAQEIERTCGVDIALRWVNGYDTTIMSFVNIVETPGGG
                     THVDGFMNAITKQIRKAVEDNARKLKVNMKDSAMKVERDDIQAGLVAVVTARVAEPQFQ
                     GQTKDVLGTAQVKPIVTRLTDKQFGEMITGSKRGYKEQSGRVLEKIVGEMHARIQSRKA
                     KEVTRRKNALESASMPAKLSDCQPGNDDVAELFIVEGDSALGTAKAARNAGFQALLPIR
                     GKILNVQKASITQMLSNKECAAIIQVVGAGSGQSFDIEQSRYHKIIMMTDADVDGAHIR
                     ILLLTLFYRYMRPLIEHGYVYAAVPPLHRIALTGSHKGEYIYTYSDDELAGKLADLDKK
                     RIGYNDDIQRYKGLGEMDADQLADTTMDPRTRMLRRIRMEDAAQASEIFSLLMGDDVPP
                     RKQFIVDNADDFDRSKIDT"
     gene            16315..17541
                     /locus_tag="I2A13_RS05480"
     CDS             16315..17541
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582790.1"
                     /locus_tag="I2A13_RS05480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_007056778.1"
                     /transl_table=11
                     /translation="MASLLLAVIYVAFISLGLPDSLLGSAWPTMSQDLNVPVAWAGGIS
                     AVISMFTIVSALLSDRMTLKFGAGKVTAVSVALTAAALAGFSVTSNYWVLLAIAIPYGL
                     GAGGVDAALNNYVAIHYESRHMSWLHCMWGVGASVGPYIMGYALSQGQGWPWGYRYIAI
                     LQVMLTVILVLSLPLWKKRGAIAVGESTDDTASSDGNAERFGTAEGVSVAERKPLGVAG
                     VLAIRGAKEILVMFFCYCAVESTAGLWASSYMVMHSGIDKITAASWASLFYVGITVGRA
                     LSGFLTMRFKDPVMIRLGQVLVFAGILTMFVPLPHHLGVVVGLVVIGFGCAPIYPCVIH
                     STPAYFGEDKSQAIVGVQMACAYVGSLLMPPLFGIIAQYATISLYPWYLLVLLVLMVAM
                     HERLRKLRG"
     gene            17585..18577
                     /locus_tag="I2A13_RS05485"
     CDS             17585..18577
                     /EC_number="2.7.1.15"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1276: PfkB domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003831735.1"
                     /locus_tag="I2A13_RS05485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ribokinase"
                     /protein_id="WP_032684915.1"
                     /transl_table=11
                     /translation="MVISEEEHEQMPEPVRERLEALKDAPGSVIVVGSMNADYTVTTKR
                     LPKPGETVNGGAMKVLPGGKGANQASAAARLGVNVQLLGAVGEDSNADFLLSKLDEAGV
                     DTADILHVEGPSGTTVITVSAEGENTIVYSPGSNAKASAGYVQSHRTTIAECAVLGLCL
                     ESPISTVIAAAQTAHDAGVTVLLNDSPFMDELPHELVEATDILLVNQHEVAQLLGLPDD
                     DVESLDWYEVAERFTDYGFDRAIVTLGASGSVVIENGRWHRVSAAQVNAVDTTGCGDSF
                     MGTVLAGLAAGHTLLQSAQIGSYVSAYAATKLGAQSAYGTAEEVIAYFS"
     misc_feature    complement(18867..18882)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.71"
                     /tool="antismash"
     misc_feature    complement(19505..19521)
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.52"
                     /tool="antismash"
ORIGIN
        1 gtcccggtgg atctgggtgt tatcgccccg gagcccgagc cggaggtgtc ggaggaagag
       61 ccgaacaaca gcccgcaggc tgcggtcgcc tccgatcttg gccctggcga atccgtggag
      121 gtccaggttg gggatacgcc gattgtcatc cgtcgcaagc tgcacatcgc cgagcctatg
      181 agcatcgacg aggcgctgta cgagatggaa ctgatcggac acgacttctt cctgttcgtg
      241 aacaaggaga ccggacgccc gtccgtcgtc taccaccgtc acggttggag ctacggcgtg
      301 ttcgaaatcg acactccgga gaatgtcaag aaagcctgaa ggtaaggcct gctgacacga
      361 tcagctgata ccgcatggac cgcttccaat atggaggcgg tccatttttt cgtaaaaagg
      421 tcgaaaaatt cgactgctga cggtgtgagg cgctatcctg atacgcggac accaccactt
      481 atcagtcaag ggagaaccca gtggtcgata ttattgacaa agccctgcga atgggtgaag
      541 gccaccagct caagaagctg gagaacgtgg ccaaggccgt gaacgctctt gaggacgaga
      601 tctccgccct ctctgacgag gatctgaagg cccaaacccc gaagttcaag caggaaatcg
      661 aaaacggcaa gagcctggac gagatcatgc ccgaggcgtt cgccaccgtg cgcgaggtct
      721 ccaagcgcac ccttggccag cgtcacttcg acgtgcagct catgggcggt gctgccctgc
      781 actggggcaa catcgccgaa atgaagaccg gtgaaggcaa gaccctggtc gccaccctgc
      841 ccacctatct gaacgctctc gaaggcaagg gcgtgcacgt ggtcaccgtc aacgactacc
      901 tcgccagcta ccagagcgag ctgatgggcc gtatctaccg tttcctcggc atgaacgtcg
      961 gctgcatcat caccgagcag aagccgccgg agcgccgcaa gcagtacaac gctgacatca
     1021 cctacggcac caacaacgag ttcggcttcg actacctgcg tgacaacatg gcatgggaga
     1081 aggccgacct cgtgcagcgc ggtcaccact acgccatcgt cgatgaggtc gattccatcc
     1141 tcatcgatga ggcccgtacc ccgcttatca tctccggccc ggccgaaggc gacgtgaccc
     1201 gctggtaccg tcagttcgcc aagctggtgc tgaagctcac ccgcgacgag gactacgacg
     1261 tcgacgagaa gaagaaggtc gtcggcatcc tggacccggg catcaccaag gtcgaggact
     1321 tcctcggcat cgacaacctg tatgagccgg ccaacaccgc cctgatcggc tacctcaaca
     1381 acgccatcaa ggccaaggaa ctgttcctgc gtgacaagga ctacgtcgtc acccagggcg
     1441 aggtgctcat cgtcgacgag cacaccggcc gtatcctgcc gggccgccgc tacaacgagg
     1501 gtctgcacca ggccatcgag gccaaggaag gcgtggaggt caaggccgag aaccagacct
     1561 tcgccaccat caccctgcag aactacttcc gtatgtacga caagctcgcg ggcatgaccg
     1621 gtacggccga aaccgaggcc gccgagttca tgaacaccta caagctgggc gtgctgccga
     1681 tcaagaccaa caagccgatg atccgcaagg accaggacga cctcatctac cgcaccaaga
     1741 aggagaagct ggccgccatc gtcaaggacg tggccaagcg tcacgccaag ggccagccgg
     1801 tgctgctcgg taccgcttcc gtggaaagct ccgaagtggt ctccaccctg ctcgacgtgg
     1861 ccaagatccc tcaccaggtc ctgaacgcca agcagcatga gaaggaagcc gccgtggtcg
     1921 ccgtcgccgg ccgcaaggga gccgtcaccg tggccaccaa catggccggc cgtggtaccg
     1981 acatcatgct cggcggtaac gtcgagttcc ttgccgacgc caagctgaag tccgagggct
     2041 actctccgga ggacaccccg gaagagtatg agaagcgttg gccgggcacc ctgaacgaga
     2101 tcaaggccca ggtcaaggac gagcatgagg aggtcaagga gctgggcggc ctgtacgtgc
     2161 tcggcaccga gcgccacgag tcccgtcgaa tcgacaacca gctgcgcggt cgttccggcc
     2221 gtcagggcga tccaggcgag tcccgcttct acctgtcgct ggaagacgat ctgatgcgtc
     2281 tgttcaacac ccagctggtg gcccaggtca tggccaaggg catggaggaa ggccagccga
     2341 tcgaggccaa gtccgtgacc aagggcgtgc gcaccgcaca gaaggccgtc gaatcccgca
     2401 actacgagat tcgtaagaac gtgctgaagt acgacgacgt gatgaacaag cagcgtaccg
     2461 tcatctactc cgagcgtcag gccgtgctca agggcgaaga catccacaag gacatcctgc
     2521 gtttcatttc cgacaccgtc gagtcctaca tcaagggtgc caacaaggga tccgagaaac
     2581 cgaaggactg ggattgggag ggcctgttca aggcgctcaa caccgtgatc cccaccaagg
     2641 tcgacgaaga cgaggtcagg aagatcgtcg gcggcctcaa gggtgcgaag gccgtcgagg
     2701 ccgtgcgcga cctgatcgtc gaggacgcca ggcagcagta cggcgaaatg gaggagacca
     2761 tcggcgagac cggcttgcgt gatctcgaac gccgcgtggt gctcgccgtg ctcgaccgca
     2821 agtggcgcga gcacctttac gagatggatt acctcaagga cggcatcggc ctgcgtggca
     2881 tgggccagcg cgacccgctg gtcgaatacc agcgcgaagg ctaccagatg tacaactcca
     2941 tgatcgaggc cattaaggag gagaccgtcc agctgctgtt ccacatcgac atcaagcagg
     3001 tcgccaccac cgacgaggcc gttgacgagg tcgaggagac cgccgaaagc gccgacacca
     3061 tcgccgtggc ctccggcccc gacgagaacg gcgaatccgt ggtcgaggcc gccgagggcg
     3121 aagtcgagga agaggacgag gatacggacg ccaagcaggc gatcgccgaa tccgcagccg
     3181 cctccggggc cggcgagtcc acgctgcctg ttgccggacc ggctccgatc agccacgccg
     3241 aaggcaaggt cccggtttcc aagcgtccga agagcgaaga gctcaagacg ccgtgggccg
     3301 acggccgcac cttccctggc actggcaaga acgccccgtg cccgtgcggt tccggccgca
     3361 agtacaagat gtgccacggc cagaacgaga agtgacgcat cgctagttag taactagctc
     3421 atggctcccc acacggggag ccattttcta tgcggttcat cagcatgtat tcgtgcggtg
     3481 ccgtacgcgc gggacggcac gcgattttcg cggattttga cgtcgtatcc cacactgttg
     3541 cgggatggca cgagtgtcaa tcgattgact agcgtggcgt cccgcagtac gaattacgga
     3601 atcgttgaaa ttccgccatt ctcggtgtta tggtgtcacg gtccgcaaag ggtgatgtgg
     3661 gacggcgcgg ctcaaacggc gaaaatgagc tgccgtcccg cagttttacg ggatagcaca
     3721 aaaaagaggc ttctgctgtt ttcgtgggtt gtcgggcgcg ccgctcgtcc aactgacgat
     3781 gtggaacccg tcgggcgcgc gttcggcgtt cgggcaccac tcatgcacgc atgagtcgat
     3841 ccaccgggcc ccgtccccgg cgacgaccct gatggaggcg cgctgttcgg gcgtcaacgt
     3901 ttggaagaac aggccgaaca cgtccctgcc gtacccgtcg tgcgcccata tcgcccgatg
     3961 ccgttcgtgg tcgacgacca cggtgatgca cgtgtggcct ttgcggtagc tggtctcgtc
     4021 cacgccgatc gacgtgaggc cgccgaacgg agacggcatc gacgctttca accgttcagc
     4081 gaccctatgg gcggcgtctt ccggcggcgc gccacgcgac gtgcggaaaa ccgcttacgg
     4141 tcttctggtt cgcgacgctc atcggccacg cgcgttccga ttcgaaatcg cgtgtggacc
     4201 ggctgcccgg ctcggcccac ggcacggaag cgaccacgac gccgcacgcc gccgccctgg
     4261 tcatgcccgc ggcgcctcct tccgcatcgg gagcatcgga gcatacccgc acgtggacgc
     4321 acccggatct cgagcacggg ttcggaacgc ggcggcgaat ccgtgatccg cgcatcctct
     4381 gcaaccatgc ctttgacgtt cggcaatccg gtgaatgggc ttttgcccag gcgttcttcg
     4441 tgattcattt ctttggtcgg aaaacagaat acgcgggaac gcccctcacg cttacgaaac
     4501 caacggctcg acgacgaacc gacatccacg aaaacagcaa tagcgccata ttttgcaata
     4561 tggaagattc agggccttga aatgacgaac cattacaatg agcggcgaac atacatcaca
     4621 cggcgtagga aacgccaagg agggcatcat ggccgagatc acatggaagt cgattctcac
     4681 caagctggtc gggggagacc accttactgc tgaggaatcg gagtggttcg tggacgacct
     4741 gatgcaaggt aacgccaatc cggccgccgt gggtgccgcg ctcgctatgc agcagcagct
     4801 gggactgacc gccgacgagg tgcgtggtgc cgccaaggcc atggtctccc acgccgtgcc
     4861 gctgaacgtt tccggtgaga ccaccgacat cgtgggcacc ggtggtgacg gcttcgccac
     4921 cgtgaacctg tccaccatgg gctccgtggc cgccgccgca gccggcgtga agatcgtcaa
     4981 gcacggcaac cgcgctgcat cttcgaagtg cggtgccgcc gatgtgctcg aagcccttgg
     5041 cctgccgctg gatctgaagc cggaagccgt cggcgaagtg ggcgacgagg tgggcatcac
     5101 cttcgcgttc gcccgcactt tccacccggc catgcgtttc gtggggccga ttcgcgccgc
     5161 gctgggcatc ccgtgcgtct tcaacatcct ggggccgctg accaaccccg ccaacctggc
     5221 acacgtggcc atcggctgcg ccaaccgcaa ggtgagcccg attatggcag ccgtgtacgc
     5281 cagccgtgga cagtctggct tggtctacac ctctcacgag ggcttggacg aacttgcccc
     5341 caccggcccg gtttccgtct gggagattcg cgacggcaag gttaccgagt ccgagttcga
     5401 tccgaccgtg gatctgggct tgaccaagat taccgtcgaa cagctcaagg gcggcgttcc
     5461 ggacgtcaac gccaacgcat tcaaggactt cctggctggc aaggacattc cgtcccgcac
     5521 caccgcactg ctcaacgccg cctccgccat cgtggccgac ggcaatctgg tcggcaacgg
     5581 cacactggcc gagcgtttcg ccgaagccta caagctggcc gaggaaaccg tcgattccgg
     5641 caaggccgaa gccctgttcg acaagtggat cgaaaccgcc aagtccaagg cgtgatacac
     5701 cgtactcagc cccctctgat gagggggctt atttgtctat atggcgactg ttatggcggc
     5761 cgcggccttg tgtgtgactg cggcgtgcgg cacttcggtt ggtgccgctg atgtgccggt
     5821 tgtgccggcc aaaggggacg tgacgatccg tctcaactgg tggggaggcg atgcccgcat
     5881 caaggccatg attgccgagt tgcaggagtc catcgacttc aattcgtgac ctatcgtggc
     5941 tcggccgcaa ttacggacga gctacgcccg caatacattg gttcgctatc tctcacacct
     6001 cgcctacaaa cagcccactg ggctgtttgc ttaacggctc agcctcagag aggggagcca
     6061 agaaccacac agcctacgcg agctacaccc tcgcgccgtc gtcgtaatag ccgccctgat
     6121 caccggggcg gtctttgcgg ttgaggattt tggccaccaa tccacccaag cccaaaatga
     6181 cgcccagccc gaagagaata ttgagcagcg cgcccaagag aggaatcgcg ttcaagccgg
     6241 ggatgaatat catcaccgct tcgcctacga gaccaatgat aagcaacgcc gtcagcagta
     6301 ccgtagacgc tttgaccgga ccaagatcag ggttcggggg agtgaagccg ccatcatcat
     6361 aggcgtccat cacattatcg gtatccagcc agctggagcc ggtgaaatca cggggacctg
     6421 actgcttgcc tttcttgccg ccacggctcg ggcgagcatc atcggtgaac gagccgatat
     6481 ccaagtcatc tacggaaagc agcgcctcct tctcgcgttt cttggcctgc ttctcgaact
     6541 ttttggcctg cctggaggaa gccacgtcat gcaagtcggc ctgatgctcg gcttcgaacg
     6601 cggcccacgc cgcatccaaa tcgttcgcgt catacgattg cggtgtatcg gcaccattcg
     6661 tggcttgtcc ggcggcaccg ggagttggcg acttggatga agtaccggaa tcctgtccgt
     6721 tgcccggcgt gggggttggc cgctctccgt cggcattgct attctggttc tcagtcataa
     6781 ctgtcactct aatcaggcga ccggccaatg gctagttcgt ctgcgggagg aatgccaagt
     6841 gctctactgg ttcttcgtca aaggtctcgg ccctattgct catcatcgtt tgagccctgc
     6901 tgtcagcgga ctgaataacg tgccgcgaga gggcggcgcg attatcgcag ccaaccatct
     6961 ggcggtgatc gatgatgctt tgattccgat tacctgcccg cgcatggtgc acttcatggg
     7021 caaagccgaa tacttcgagg gcaagggatt gaagggcaag ttcaagaagt ggtggttcac
     7081 gtccgtgggc gtgttcccgg ttgatcgttc tggtggcagc aagtcgcttg gcgcactcga
     7141 gcacgcacgc gagattatcg aggatgggca cctgttcggt atccacattg aaggcacgcg
     7201 cagcccggat ggtcgactgt acaagggaca caccggtgtc gcacgacttg cgctcgagac
     7261 cggttgcccg attgtgccga ccgccatcat cggctcggac aagctgcagc cgattggcac
     7321 gtcgattccc aagaagggca agaccaaggt tctgtatggc aagccgattg aggttgccaa
     7381 gaagccggcc gatgagatta cacatgatga tttgcgttcg ctgaccgatc gtgtttccgc
     7441 cgcaatccag aaaatgagcg gtcaggaatt cgtgaacgaa tacgcccaga aggtcaaggc
     7501 cgagctcaag gcccagcagg ccgccgaaga agccgaataa tatgtgttgg ctcccctcag
     7561 cgaggggagc cagttgatta ctctcacacg atggcgaggg tgatgacggt ggggttgccg
     7621 ttggcatcgc gcaggcgaat cttgccgccg ttggatagat ccgtatcccc gcttttcacg
     7681 gtacgtaccg tgccgaacag cccaccgagc accttgtcgg ttttcacctc gaaaccgaga
     7741 gcctcaagcg tcttggtcac ggcgctttcc tgtttgccga caaggccgga cggtatcgtc
     7801 gccatctccg gacccttgga aatcaccacg tccacgctat cgccccactt caactgggct
     7861 ccggcttcct gagacgcgga aatgacttgg ccggcctcga ccttgtcatc gtactgttcg
     7921 gtgacgttgg ccgtgagctt caactcgccg agcgcggctt gcatctcatc ctgggtctta
     7981 ccgacgatgt taggcatggt caccggcatc ggtcccttgc tcaaagtaac cgtcacctca
     8041 tcgttgtgct tggctgcagt accgggatca ggggagatcg tcaacgccac accctgcggc
     8101 gtgtccatcg aatactcgtc cttcgactcg tcgtgcttga cattatcgaa gccagccctc
     8161 ttgagcgcgt tcaacgggtc tttgccattg gccgaattcg cgtcgagaat atccttcggg
     8221 atggtggcca tccgcacgcc cttggacacc accaccgtaa gctcctgatt ggctcgcttg
     8281 cttatgcggc tgttcacaac ggccttggtc ttgttcacgg aagaagagat gatcttgcct
     8341 tccgccacgt cgtcgttgta ttccttgtgc gtcttgtagg ggatgccgag agccttcaac
     8401 gtgctttcat acgtggccca atccgcgccg gccagcgagc attcggtgcc tgcattcgca
     8461 tcgcaggtca cgtcatccgg cttgggcaca ctccaatagc tgcccggtcc ggcaaaccac
     8521 caccagccgg cggttccgcc gattgcagcc agtaccagca ctatcaccag tacgatgatg
     8581 agcggttttt tgctgcgctg cttctttggc ccggtgccat tggaagcgac gtccggctca
     8641 tcggaaaggg caagcgctcg ggtgaaggca tcgccggcct gaggcagcac ttgtgtctcg
     8701 ctgccggact gtgccatgat ctgcgtctta tggggagccg aagcatcggc taacatcgac
     8761 gaatcaagtg ccgtggtggg ggcgaccgga ggcgccgggg gattgacgtc tgcggcgcca
     8821 ccggtatcgg cggtattgct tgcggcatca tcgaccaaaa atgccggtac ggacggctcg
     8881 aacgttgctg caccggcagg tacgccggtc agcggggcct gctcggcgat attgcctacc
     8941 aatgctgcag cggtggcatc ggtatgatcg cctccgatgg gttcggcatg caaacggtat
     9001 tgccacgcct ccagcggcaa tttggcggcc aactggctca attcctcagc tgctgccgcg
     9061 ccatcggtgg ggcgggcatc cacctgacgc gcggtcagat gggcgatgaa cgccgccacg
     9121 gacggatcaa tgccctgaca gaccgtggcc acggacggca cgtcctcatg aacatgcttg
     9181 aaaacaagcg tcaccggatt atccgaatcg aacggcacct tgccggtcag catctcccat
     9241 gccatgatgc ccaccgaata caggtcgcct tgcgcggtgg cctgattgtt ctcgatcatt
     9301 tcgggagcca gataagcggc ggtgcccaat agcatgccgg tggagctcaa tgtggcctga
     9361 ctggcggcct tggccagacc gaaatcagtg atctgcacat ggccgcgatc attgatcaaa
     9421 atgttctcgg gcttgatgtc acggtggacc acgccggcgc gatgcgcgga agccagacca
     9481 tccagcgtct ccgcgaccac gcgcagcgtc tcacgtacgg agaatgtgcc ttgggcgttc
     9541 atctcatgcc gcagattgac gccatgcaca tattccatga ccagaaaatc aagtccattg
     9601 aactcgccgg tgtcatacac ctgaacgatg tgcgggttgg cgatggacgc ggccgaattg
     9661 gcctcgcggc ggaaccgttc gacgaactgc tcccgatgcg ggcctttggc gagttgtgtg
     9721 tgcatgacct taatcgccac ggtgcgcccc agacgctcat cgacggcctc atacacggtg
     9781 gccataccac cctcggcgat attgcgcacg atgcggtagc gtccctcaat catctgcgcg
     9841 ggctcgttct cactcatgtt gtatcgtacg gttcctttaa tcacgactaa acatgttcag
     9901 tgtacatggc cccggttacg cggccggagc atcagcgcat agttttcacg ggttttcacc
     9961 ggtgttcatg gtctcatggt ccggcggctc gcattgtgga ctatcgcaag gattcgggga
    10021 cgaatcgcgc acaagcctcg gtcagacggc gttgcgcttc ggaattcagc tcaagaccgg
    10081 caatcgcctt acgggactca ccccataggg cagcgatgcg ctcgcgggat cgatcaagag
    10141 caccggtcga cgtgaacagt tcaatggtgc gggccacctg gttgtcattg cgacaatctt
    10201 cttcgaagat ggcaatcaga tcggccttat ccgccgtatc tgccgcagac agagcatcag
    10261 ccaacagcac tgtgcgtttg ccttctcgga tgtctccgcc aactggtttt ccggtattgc
    10321 ggcttgatcc gacgacatcc aacaggtcat ccgccagttg gaacgccagt ccgagcggcc
    10381 ggccgacggc caacgcgcaa tgcctggcct gatccgggct ctctcctgcg gccagcagtg
    10441 caagcagcag tggagcgatg gtggtgtaac tggctgtctt ccagcggaat acgttcaatg
    10501 aggcgttcgc cagagcttcg ggattggaca gtggggtcat ctcgacggcc aagtccagta
    10561 cctggccgat ttccacctcg cgttgcatgg taagaaacgc ctcgttcagg gcatcggtgt
    10621 tgggaagacg gctggcggac cggcgtgtga tttcaatgca ggcggtggcc agaatgtcgc
    10681 cgagcatcag gcccaagcca cggcctatgg caccgctgtg tacggcctgt tccaaggcac
    10741 gatgtgcgga aggcctgccc cggcgcagat cggattcgtc gatgatgtcg tcatgcacca
    10801 gtgcggccgt ctgaaacact tcaatggcgc aggccaggtc gatcatggcg tcacgccgat
    10861 caggcgtcac gtcgccggca agaatatcga aggcgtccaa cgcgagcagc gcacgcagtc
    10921 gtttgcctcc ttggctggag gaggcggctt ggtccacaac ctgttccatc accggtttca
    10981 gggcgtcggc aacctcgtcg gtggcggggg cggcagtcag ttcccgcacc aactgaatga
    11041 tgcgtggctc gatgatttcg cggttgttag cagttgtgca catgcctaat aacttatcca
    11101 cagggtttga agaacacaag aacgttggga atcaagggtt ttgaagctag aaccacaatg
    11161 gtcgtttttt cctgcgtcat acggcgtttt cagccatagt gggcacacac cccctcggcg
    11221 catgagctat ccggctcggc gcgcaccaca acaccaatag attgcacacc attagtcaag
    11281 gaggacatga tgaacaacaa cacgccgcac aagccgcgta agagccgaca cgccaaacgg
    11341 cacgccgccg agcagacgga gccctttggc tttgacgagt tggagacgag atcacaggaa
    11401 tttcgcaatg atcgacgact tcgaggcgcg caacaggcta atgccgactg ggtggccgaa
    11461 ccattggacg aatggctgtc ttgtcttgat gccgggaaca ccgacctagg ccgagagacg
    11521 atggccgcgt ttgcggtggg catgcgtgag accttatcga ttcgtgatgc gctgattctc
    11581 tcgttggtta ttgatgaaag ccgttgcccc aagtcatggc ttatggaatt cgcggcgcga
    11641 ccgcatctgg cgcgtacgag aacacgaatg ggcgagctgc tgacttgcgc attcgaggat
    11701 gaaggcatga tgccggataa ggagcgttgc catgccggca tcggtatgtt gctcgatatc
    11761 gccgaagcct caccggtgcc gtactgcata cagccattgg ccgtaatcgc ctacacctta
    11821 tggtggctgg gcgatccgcg agcaatggtg ttcgccttgc gatgcctgct gctcgatgag
    11881 gattgttcgc tggcggcgat gatattctca gccgccgatc gaggcgtggc accggcatgg
    11941 tgctcgggct gagtgaacag aaacggcgga tttccgaggt tttacgcgcg atgcgcggaa
    12001 ggaaagcgtg tggtgatcgg caacacgtcg ggcaacgatg tgggaataat cgtccgtgga
    12061 gggtggttta ctcacatagt tgacgatttg cccttgccag aagtgggccc atgtggtata
    12121 tgcacttcac ggcgagagat caggaggagc agttttggcc acgaaggaaa cgacggcaac
    12181 caagcagaca gccgagtcca gcgaggagac cgagaccaag aagaaggcca gctctacgac
    12241 caccgcacgc aagacttccg ccaagaagtc caagtccgca gagccctcga aaaagaccac
    12301 gcgcaagtcc gctgcgaaga aaaccgcgaa gaaggccaag gacgatgagc tcgtcaaaga
    12361 cgaggacact caggtcgatg acgagcttga tgacgatgcc gaggtcaacg acgacgattt
    12421 ggacgttgac gacctcgacg aggatcttga cgacgttgac gaagacgatg ccgacgacga
    12481 agacctcgat gaggacgatg ccgacgacga tgatgtggaa gacgacgagg acgaggaaga
    12541 agccaagtcc aaggcacccg agcagcccaa ggaaaagggt gcctacgtgg tctccgacac
    12601 ggatgatgag gaagagaaca tcatcccctc gggcaatccg aagcgtcgtg tgattgcggc
    12661 cggcgcaacc gccgacccgg tcaaggacta cctgaagcag atcggccgtg tgagcctgct
    12721 gaatgccgag caggaagtcg acctgtccga gcgcatcgag gccggcctgt acgcccagca
    12781 cctgctcgac accgaaagcg aaggcatgga gttcaagcgc aagcgtgaac tcaagtgggc
    12841 cgccgccgac ggcaagaagg ctaaggacca tctgctggaa gccaacctgc gacttgtggt
    12901 ctcgctcgcc aagcgctaca cgggtcgcgg catgctcttc cttgatttga tccaggaagg
    12961 caaccttggt ctgattcgtg ccgtcgagaa gttcgactgg aagaagggct tcaagttctc
    13021 cacctacgcc acgtggtgga ttcgtcaggc catcacccgt gccatggccg atcaggcccg
    13081 caccattcgc gtgcctgtgc acatggtcga agtgattaac aagctgtccc gtgtacagcg
    13141 ccagatgctg caggatctgg ggcgtgaacc tacgccggac gagctggccc gcgagctgga
    13201 tatgccggtt gagaaggtgc aggaagtgca gaagtacggc cgtgagccga tttccctgca
    13261 caccccgctt ggcgaggatg gcgattccga gttcggtgac ctcattgagg ataccgacgc
    13321 catcgctccg tccgacgccg tggccttctc cctgttgcag gaacagttca agcaggtgct
    13381 ggaaaccctg tctccgcgtg aggccggcgt catcaagatg cgctatggcc tggaagacgg
    13441 ccagccgaag acgctggacg acatcggccg tgtgtatggc gtgacccgtg aacgcatccg
    13501 tcagatcgag tccaagacca tgtccaagct gcgccacccg tcccgttcgc agaccctgcg
    13561 cgacttcctg gatcaatgat catctggctc ccctctgtga ggggagccaa gcatttgaag
    13621 agaaaaatag gactgtatgc ccaaagaaga atacggcgcc aaggacctag ctgttcttga
    13681 gggcttggat gcggtacgca agcgtccggg catgtacatc ggcaccaccg actcgcaggg
    13741 tcttatgcac tgcctgtggg aaatcatcga caactccgtg gacgaggcct tggccggctt
    13801 ctgcaacgac attgtggtca ccctgcacac cgatggttcc gtggaagtcg cggacaatgg
    13861 ccgaggcatc ccggtggaca aggaaccgaa aaccggcctg tccggcgtgg aggtcgtgct
    13921 gaccaaactg catgccggtg ccaagttcgg caactcgtct tataacgccg tcggtggcct
    13981 gcacggcgtg ggctcctccg tggtcaatgc cctgagctcc cgactggacg tcgaagtgga
    14041 tcgtgacggc aagacgcacc acatgacctt ccaccaaggt caccctggcg tatacacgga
    14101 tgccgacccg gccaatccgt ctcccgattc cccgttcaag cgcactcgca agaaccgacc
    14161 caccgagctg gaaatcatcg gcaaggtatc tcccaaaacc actggtaccc gtattcgtta
    14221 ctggtatgat ccggagatct tcaacaaaac cgccgagttc tcatacgaac agctcatcga
    14281 ccgcgtgcgt cagaccagct tcctggtgcc cggcctcaaa atcaccatca tcgacgaaaa
    14341 cgtaccggaa acggcggcga ctgacaccgt cgaggccact gatgacacaa cagtcgaacc
    14401 tgcgcaagac caggcaccgg ccttggatgt ctcgtccagc gatgccgaac tcttcgacga
    14461 ctccgccgaa tcccagtccg acgatgccga gagcccggcc gaggaggact tctcgctgac
    14521 ggccggcgac caggtcgtcg atggcgcgtt cggcgaacag cccgcgcacc cgcgcgtcgt
    14581 ggaattcctg cacacaggcg gcgtcaagga cttcgtcgac ttcctgtcca aaggcgagcc
    14641 gatctccgac atctggcgca tacaaggcga gggcacctat aaggaggaga cccaggccgt
    14701 gggtgagggc ggcgaactgc acgcccagga aatcgaacgc acctgcggcg tggacatcgc
    14761 cctgcgatgg gtcaacggct atgacaccac aatcatgagc ttcgtgaaca tcgtggagac
    14821 gcccggcggc ggtactcacg tggacggttt catgaacgcc atcaccaagc agattcgcaa
    14881 ggcggtggaa gacaacgccc gcaagctcaa ggtgaacatg aaggactccg ccatgaaggt
    14941 ggagcgcgac gatatccaag ccggactcgt ggccgtggtc accgcacgcg tggccgaacc
    15001 tcagttccaa ggccagacca aggacgtgct tggcaccgcg caggtcaagc cgatcgtcac
    15061 ccggctcacc gacaagcaat tcggcgagat gatcaccggc tccaagcgcg ggtacaagga
    15121 acaatccggc cgcgtgctgg agaagatcgt cggcgaaatg cacgcacgca tccagtcgcg
    15181 caaggccaag gaggtcacgc gccgcaagaa cgcgctcgaa tccgcctcga tgccggccaa
    15241 actctccgac tgccagccgg gcaatgacga cgtggccgaa ctgttcatcg tcgagggtga
    15301 ttccgcactc ggtaccgcca aggccgcccg caacgccggt ttccaggcac tgctgccgat
    15361 tcgaggcaag attctgaacg tgcagaaggc gtcgatcacg cagatgctct ccaacaagga
    15421 gtgcgcggcc atcatccaag tggtgggtgc cggctccggc cagagcttcg acatcgaaca
    15481 gtcgcgctat cacaagatca tcatgatgac cgatgccgat gtggatggcg cgcacatccg
    15541 tatcctgctg ctgacgctgt tctaccggta catgcggccg cttatcgaac acggctacgt
    15601 gtatgccgcc gtgccgccgc tgcaccgcat cgcgttgaca ggctcgcaca agggcgaata
    15661 tatctacacg tattccgatg acgagctggc cggcaaactg gccgacctcg acaagaagcg
    15721 catcggatac aacgacgaca tccagcgata caagggtctg ggcgagatgg acgccgacca
    15781 gttggcggac accaccatgg acccgcgcac gcgtatgctg cgccgcatcc gcatggagga
    15841 cgccgcccag gcatccgaga tcttcagcct gctgatgggc gacgacgtgc cgccgcgcaa
    15901 gcagttcatc gtcgacaacg ccgacgattt cgaccgctcc aagatcgata cctgaccgtc
    15961 ctatctcaag ctccctctga tgagggggtg tcagcgaagc tgactggggg agaggcctcg
    16021 ggtggtagcg ggctactcgt atttctctcc cctcagtccg tctttggcgg acagctattt
    16081 cgtcagaggg tgctgcggta tatcatcagg atttcatcga gtgcaccgcc gccgattatc
    16141 tcaaggacaa ctacgacgtc atcggctttg acccgcgcgg cgtagccgat tccacgccgg
    16201 tgtactgcgg gcaagactcc aaactgtgat gtttgcgtgt tgctgaagcg ctattcgata
    16261 gactgatgac atcgttatca caaccggtgc cgtactgtga atgggagatg ggacatggcc
    16321 agtctgttgc ttgccgtcat ctatgtggcg tttatcagtc tgggattgcc ggattcgcta
    16381 ctcggatccg catggcccac catgagccag gatctgaacg tgccggtggc gtgggccggc
    16441 ggcatctccg cggtgatttc catgttcacg atcgtctcgg cgctgctttc ggaccgaatg
    16501 acattgaaat tcggggcagg caaggtcacc gccgtttcgg tggcgttgac tgcggcggcc
    16561 ttggccggtt tctccgttac ctcgaattac tgggtgctgt tggccatcgc cataccatac
    16621 ggccttggcg cgggcggagt ggatgcggcg ctcaataact acgtagccat tcactacgaa
    16681 agccggcata tgagctggct gcactgcatg tggggcgtcg gcgcatcggt cggcccctac
    16741 atcatgggct atgcgctctc tcaaggccaa ggttggccgt ggggctatcg gtacatcgcg
    16801 atcctgcagg tgatgctgac cgtcatcctg gtgctgagcc tgccgctgtg gaagaaacgt
    16861 ggcgcaattg cggtcggcga atcgaccgat gacaccgcat catctgacgg caatgccgag
    16921 agatttggca ctgctgaagg ggtgtctgtt gccgaacgca aaccgctcgg cgtggccggc
    16981 gtgctcgcca ttcgcggtgc caaggaaatc ctcgtgatgt tcttctgcta ctgcgccgtc
    17041 gaatctacgg cgggcctgtg ggcctccagc tatatggtga tgcacagcgg catcgacaag
    17101 atcaccgccg ccagctgggc cagcctattc tacgtaggca tcaccgtggg ccgtgcgctt
    17161 tccggcttcc tgaccatgcg attcaaggac ccggtcatga ttcgcctcgg ccaagtgctg
    17221 gtgttcgccg gcatcctcac catgttcgtg ccattgcctc accaccttgg cgtggtcgtt
    17281 gggctggtgg tcatcggctt cgggtgcgcg ccgatttatc cgtgcgtcat ccactcgacg
    17341 ccggcctact tcggcgaaga caaatcgcag gccatcgtcg gcgtgcagat ggcctgcgcc
    17401 tatgtgggct cactgctcat gccgccactg ttcggcatca tcgcgcaata tgcgacaatc
    17461 agtctctacc cgtggtattt gctggtgctc ttggtgctga tggtggccat gcacgagcgt
    17521 cttcgcaagt tgcgcggctg atcatatgcg catttgggcc cgacgcctac tatgcttgga
    17581 gcccatggtg ataagtgaag aagaacatga acagatgccc gagcccgtac gggaacgatt
    17641 ggaagcgctg aaggatgcgc cgggctcggt aatcgtggtc ggctcgatga acgccgatta
    17701 caccgtaacc actaaacgac tacccaagcc aggtgaaacc gtcaatggtg gtgccatgaa
    17761 agtgctgccg ggcggcaagg gcgcgaatca ggcgtctgcc gccgctcgtc tcggtgtcaa
    17821 tgtccagttg ctgggcgcgg tgggcgagga ctccaacgcg gacttcctat taagcaagct
    17881 ggatgaggcc ggcgtcgaca ccgccgatat cctgcatgtc gaaggcccca gcggcaccac
    17941 ggtaatcacc gtgagcgctg agggcgagaa caccatcgtc tattcgccgg gttccaatgc
    18001 caaggcaagt gccggctatg tgcaatcaca tcgcacgacc atcgccgaat gcgcggtatt
    18061 gggactgtgt ttggagagcc cgatctccac cgttatcgcc gccgcgcaga ccgcgcatga
    18121 cgctggcgtg accgtgctgc tcaacgactc gccatttatg gacgagctgc ctcacgaact
    18181 ggtggaagcc acggatattc tgctggtcaa ccagcatgaa gtggctcaac tgctgggcct
    18241 gccggatgat gacgtggaat cactcgactg gtatgaggtc gccgaacgat tcaccgatta
    18301 cggattcgac cgggcgatag tgacgcttgg tgcgtcggga tcggtggtga tcgagaacgg
    18361 acgttggcat cgcgtatccg ccgcgcaagt caatgcagtg gacaccaccg gttgcggcga
    18421 ctcgttcatg gggaccgtgc tggctggctt ggccgccggt catacgctgc tgcaatccgc
    18481 gcaaatcggc tcctacgtct ccgcctatgc ggccaccaag cttggtgccc aaagtgccta
    18541 cggtaccgcc gaagaagtca tcgcctactt cagctgacct ataatcgtac atatgtacga
    18601 aagcctgagc ctgttttccg aacccaccaa gatgtggttc gagcatgcct tcggtcagcc
    18661 gaccgaggca caggctcagg catggccggc aatccattcc ggtcgcaatg tcttggtcat
    18721 cgcccctacc ggttccggta aaacattggc cgcgtttctc tcagccatcg accgattgat
    18781 gaccgtgcca cgcacgcggc gtgccggtgt gcgtgtgctg tatatctcgc cgttaaaggc
    18841 gctcgcggcc gatgtggcca agaacctaga acagcctttg gagggcattg ctgcacaatg
    18901 cgaggcgcaa gggctgcccg tgccgaagat cgcggtcgcc actcgttcag gcgacaccac
    18961 cgcacaggag cggcggcgca tagcctccca tccaccggat atcctcgtta ccacgccaga
    19021 atcgttgtat ttgttgctca cttcgaaggc agggcggatt ctcggcactg tcgatacggt
    19081 gatcgtcgat gagattcatg cagtggcggg caccaagcgc ggcgcacatt tggcggtaag
    19141 tctggaacgg ctggagaacc ttgtcacaga atcgcgcaag cgagatgcaa tcgatgcaga
    19201 tgctgatgag ggcggtgatg ccgccgtcga tgctggccgc ggcgatcgtc atatgcagcg
    19261 catcggtcta tccgctacgg tgaatccgcc tgaaaaggcg gcgcgattcc tgggcggcgg
    19321 gcaaccggtt accatcgtga atcccggtgg gcggcctgca atggatttgc gcatgattga
    19381 gccgttggaa aacatgcgtg atttgcagtc cgtgaatgcc aagcagcgtg tcggtggcgt
    19441 ggatgctgaa cggtccgctc cgcatatcag tggtgtcacg ccggccatgc agcgacttgc
    19501 tgaacgcagg ggcattgtgc cggtggatga tagagatgtt tcttccactt ctggggacga
    19561 ttctgatgcg agtgatgcgt tcgattcctc cgcgttggtt ggagcggcgg gcgaccgtac
    19621 ctccggttcc atttggcccg tggtggaacg cagtattttg gatgagattc tcgcgcaccg
    19681 cactacattg gtgttcgtta attcgcgtgg tgtggctgag aaactcactg cgcgcttgaa
    19741 cgatctgtat gcgcagaccc ggcatggcac caatcccgac actgtgcggg atctgggctc
    19801 tccggaaggc cgcgaagggt tctccacgca ttacgatgct gtggtcggct ccaccaccat
    19861 gcttgtcggc tcacatgagg gcgatgatgt catcgccatg gctcatcatg ggtcggtttc
    19921 caaagaccgt cgcaaaatga ttgaggaacg gcttaaacgt ggtgaattgc gttgcgtggt
    19981 ggccacctcc agtttggagc tcggcattga tatgggctcg gtcgacttgg tgattcaagt
    20041 cgatacgccg ttgtcggtct cctccggatt gcagcgtgta ggccgtgccg atcatcaggt
    20101 cggtggcgtc tctcacgcat tgttctaccc gctcacccgt cagcagattg tcactggggc
    20161 cgcaagtctg gaagccatga ttgccggcga tatagaacca ttggccgtgc cgcgcaatcc
    20221 gctcgatatt cttgcccagc agaccgtggc cgctgccgcg atgcacgatt tgaacgctga
    20281 tgactggtat gccacagtgc gtcgtgctgc gccgtttgcc gaactgccac gtgacatgtt
    20341 cgatgcggta atcggcatga tgtccggcga atacaacagc gaggaattct cggccttccg
    20401 gccgcgactg gtatggaatc gggataatgg cctgatttcc gcccgtcccg gttcgcagaa
    20461 agtggccgtt acctctggcg gtacgattcc cgatcgaggg ctgtacaccg tggtcctgcc
    20521 tgaggcggac gccggcaaag gccagcgtcg cgtcggcgag ttggatgagg agatggtcta
    20581 cgaatcgcgc gtgggcgatg tcattacgct cggcacctcc acatggcaga ttcaggaaat
    20641 cacccgtgat cgtgtggtcg tgactccggc tccgggccgc accgcccgcc tgccgttctg
    20701 gcacggtgaa ggcgctggcc gtgactacgg tttcagccgt accatagccc gcttcacccg
    20761 agaaatcgcc gccggattag acgtgaagcg gactgagggg aggtctgccg cagaaggtcc
    20821 agctgtccca accttcatcc caacaatcct cacccgtctc caccacgatg gcctagatgc
    20881 caacgccatc accaacttgg cccgcctact ctccgagcaa caagccgcca ctggtgcagt
    20941 ccccagcgac caaaccctta tcgtggagcg cacacgcgat gaagacggcg gatggcgcat
    21001 cgtgctgtta agccccttcg gccgccgcgt tcacgaaccg tggtccatgg ccatcagccg
    21061 cagactgcgc caacgctacg gtt
//
